Wound Repair and Regeneration最新文献_第4页

Effects of a bioengineered allogeneic cellular construct on burn-related macrophage phenotype. 生物工程异体细胞构建物对烧伤相关巨噬细胞表型的影响

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-10-03 DOI: 10.1111/wrr.13227

Beatriz Hernaez-Estrada, Lindsay A Steele, Kara L Spiller

{"title":"Effects of a bioengineered allogeneic cellular construct on burn-related macrophage phenotype.","authors":"Beatriz Hernaez-Estrada, Lindsay A Steele, Kara L Spiller","doi":"10.1111/wrr.13227","DOIUrl":"10.1111/wrr.13227","url":null,"abstract":"Bioengineered allogeneic cellularised constructs (BACC) exert pro-healing effects in burn wounds and skew macrophage phenotype towards a predominately reparative phenotype. However, whether BACC can modulate the phenotype of dysregulated macrophages, like those present in burn wounds, is not known. To better understand the macrophage modulatory characteristics of the BACC, primary human macrophages were polarised to the M2b phenotype, an immunosuppressive phenotype relevant to burn wounds, by simultaneously exposing macrophages to polystyrene plate-coated immunoglobulin G and the endotoxin lipopolysaccharide (LPS). The resulting macrophage phenotype upregulated both inflammatory and reparative genes, and increased secretion of the M2b marker CCL1 compared to five different in vitro macrophage phenotypes. M2b macrophages were cultured with the BACC in the presence or absence of LPS to mimic infection, which is a common occurrence in burn wounds. The BACC caused up-regulation of reparative gene sets and down-regulation of pro-inflammatory gene sets, even when LPS was present in the cell culture media. Co-cultures were maintained for 1, 3, or 5 days in the presence of LPS, and by day 1 both non-activated macrophages and M2b macrophages exhibited signs of endotoxin tolerance, as demonstrated by a reduced secretion of tumour necrosis factor α (TNFα) in response to fresh LPS stimulus. The BACC was not able to prevent endotoxin tolerance, but reparative genes were upregulated in macrophages chronically exposed to LPS. These results suggest that the BACC can promote a reparative phenotype in dysregulated macrophages relevant to the pathophysiology of burns.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"992-1007"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-drug resistant Staphylococcus epidermidis from chronic wounds impair healing in human wound model. 来自慢性伤口的耐多药表皮葡萄球菌会损害人体伤口模型的愈合。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-10-22 DOI: 10.1111/wrr.13231

Miroslav Dinić, Rebecca Verpile, Jamie L Burgess, Jingjing Ming, Jelena Marjanovic, Carmen Nicole Beliz, Lisa Plano, Suzanne Hower, Seth R Thaller, Santanu Banerjee, Hadar Lev-Tov, Marjana Tomic-Canic, Irena Pastar

{"title":"Multi-drug resistant Staphylococcus epidermidis from chronic wounds impair healing in human wound model.","authors":"Miroslav Dinić, Rebecca Verpile, Jamie L Burgess, Jingjing Ming, Jelena Marjanovic, Carmen Nicole Beliz, Lisa Plano, Suzanne Hower, Seth R Thaller, Santanu Banerjee, Hadar Lev-Tov, Marjana Tomic-Canic, Irena Pastar","doi":"10.1111/wrr.13231","DOIUrl":"10.1111/wrr.13231","url":null,"abstract":"Venous leg ulcers (VLUs) represent one of the most prevalent types of chronic wounds characterised by perturbed microbiome and biofilm-forming bacteria. As one of the most abundant skin-commensal, Staphylococcus epidermidis is known as beneficial for the host, however, some strains can form biofilms and hinder wound healing. In this study, S. epidermidis distribution in VLUs and associated resistome were analysed in ulcer tissue from patients. Virulence of S. epidermidis isolates from VLUs were evaluated by whole genome sequencing, antimicrobial susceptibility testing, in vitro biofilm and binding assays, and assessment of biofilm-forming capability and pro-inflammatory potential using human ex vivo wound model. We demonstrated that S. epidermidis isolates from VLUs inhibit re-epithelialization through biofilm-dependent induction of IL-1β, IL-8, and IL-6 which was in accordance with impaired healing outcomes observed in patients. High extracellular matrix binding ability of VLU isolates was associated with antimicrobial resistance and expression levels of the embp and sdrG, responsible for bacterial binding to fibrinogen and fibrin, respectively. Finally, we showed that S. epidermidis from VLUs demonstrate pathogenic features with ability to impair healing which underscores the emergence of treatment-resistant virulent lineages in patients with chronic ulcers.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"799-810"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal microbiome changes in axolotl limb regeneration: Stage-specific restructuring of bacterial and fungal communities with a Flavobacterium bloom during blastema proliferation. 腋龙肢体再生过程中微生物群的时间变化：在胚泡增殖过程中，细菌和真菌群落与黄杆菌大量繁殖的特定阶段重组。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-08-06 DOI: 10.1111/wrr.13207

Hanne Altın, Büşra Delice, Berna Yıldırım, Turan Demircan, Süleyman Yıldırım

{"title":"Temporal microbiome changes in axolotl limb regeneration: Stage-specific restructuring of bacterial and fungal communities with a Flavobacterium bloom during blastema proliferation.","authors":"Hanne Altın, Büşra Delice, Berna Yıldırım, Turan Demircan, Süleyman Yıldırım","doi":"10.1111/wrr.13207","DOIUrl":"10.1111/wrr.13207","url":null,"abstract":"The intricate relationship between regeneration and microbiota has recently gained attention, spanning diverse model organisms. Axolotl (Ambystoma mexicanum) is a critically endangered salamander species and a model organism for regenerative and developmental biology. Despite its significance, a noticeable gap exists in understanding the interplay between axolotl regeneration and its microbiome. Here, we analyse in depth bacterial 16S rRNA amplicon dataset that we reported before as data resource and profile fungal community by sequencing ITS amplicons at the critical stages of limb regeneration (0-1-4-7-30-60 days post amputation, 'dpa'). Results reveal a decline in richness and evenness in the course of limb regeneration, with bacterial community richness recovering beyond 30 dpa unlike fungi community. Beta diversity analysis reveals precise restructuring of the bacterial community along the three phases of limb regeneration, contrasting with less congruent changes in the fungal community. Temporal dynamics of the bacterial community highlight prevalent anaerobic bacteria in initiation phase and Flavobacterium bloom in the early phase correlating with limb blastema proliferation. Predicted functional analysis mirrors these shifts, emphasising a transition from amino acid metabolism to lipid metabolism control. Fungal communities shift from Blastomycota to Ascomycota dominance in the late regeneration stage. Our findings provide ecologically relevant insights into stage specific role of microbiome contributions to axolotl limb regeneration.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"826-839"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital ulcers associated with scleroderma: A major unmet medical need. 硬皮病相关的数字溃疡：尚未满足的重大医疗需求。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-09-26 DOI: 10.1111/wrr.13224

Angha Naik, Richard J Stratton, Andrew Leask

引用次数: 0

Vitabiotic: An alternative approach to diabetic foot. Vitabiotic：治疗糖尿病足的另一种方法。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-09-25 DOI: 10.1111/wrr.13222

Hüseyin A Erdem, Nazlıhan Yalçın, Arda Kaya, Meltem Taşbakan

{"title":"Vitabiotic: An alternative approach to diabetic foot.","authors":"Hüseyin A Erdem, Nazlıhan Yalçın, Arda Kaya, Meltem Taşbakan","doi":"10.1111/wrr.13222","DOIUrl":"10.1111/wrr.13222","url":null,"abstract":"Diabetic foot ulcers and infections are complications that can result in significant morbidity such as the need for amputations, especially in uncontrolled diabetes, thereby profoundly impacting quality of life. While traditional treatments like wound care and antibiotics are effective, there is growing interest in the role of micronutrients (such as vitamins C, D, E, zinc and magnesium) in improving outcomes. This study aims to evaluate how these micronutrients affect diabetic foot infections and the need for amputation, offering insights to enhance overall prognosis. Patients who were hospitalised with a diagnosis of diabetic foot infection in the Infectious Diseases Department of Ege University Faculty of Medicine Hospital between 1 April 2022, and 31 January 2023 were included in the study. The patients' socio-demographic information, characteristics of diabetic wounds, operation history, as well as their levels of micronutrients recorded on the case report form. A total of 202 patients were included in the study. The most common micronutrient deficiencies were vitamin D (69%), vitamin C (64%) and zinc (49%). The amputation rates were significantly higher in patients with deficiencies vitamin C, vitamin A and vitamin D (p < 0.005). Our research revealed a significant prevalence of vitamin deficiencies among the participants, and we observed a noteworthy correlation between amputation rates and these deficiencies. Although these findings show promise, it is essential to emphasise that micronutrient supplements should not replace traditional treatments but should rather be considered as a warning sign for preventing complications, particularly amputation or extremity loss.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"890-894"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing mechanical and enzymatic isolation procedures to isolate adipose-derived stromal vascular fraction: A systematic review. 比较机械和酶分离脂肪基质血管部分的方法：系统综述。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-10-24 DOI: 10.1111/wrr.13228

Mustafa Uguten, Nanouk van der Sluis, Linda Vriend, J H Coert, Martin C Harmsen, Berend van der Lei, Joris A van Dongen

{"title":"Comparing mechanical and enzymatic isolation procedures to isolate adipose-derived stromal vascular fraction: A systematic review.","authors":"Mustafa Uguten, Nanouk van der Sluis, Linda Vriend, J H Coert, Martin C Harmsen, Berend van der Lei, Joris A van Dongen","doi":"10.1111/wrr.13228","DOIUrl":"10.1111/wrr.13228","url":null,"abstract":"The stromal vascular fraction of adipose tissue has gained popularity as regenerative therapy for tissue repair. Both enzymatic and mechanical intraoperative SVF isolation procedures exist. To date, the quest for the preferred isolation procedure persists, due to the absence of standardised yield measurements and a defined clinical threshold. This systematic review is an update of the systematic review published in 2018, where guidelines were proposed to improve and standardise SVF isolation procedures. An elaborate data search in MEDLINE (PubMed), EMBASE (Ovid) and the Cochrane Central Register of Controlled Trials was conducted from September 2016 to date. A total of 26 full-text articles met inclusion criteria, evaluating 33 isolation procedures (11 enzymatic and 22 mechanical). In general, enzymatic and mechanical SVF isolation procedures yield comparable outcomes concerning cell yield (2.3-18.0 × 105 resp. 0.03-26.7 × 105 cells/ml), and cell viability (70%-99% resp. 46%-97.5%), while mechanical procedures are less time consuming (8-20 min vs. 50-210 min) and cost-efficient. However, as most studies used poorly validated outcome measures on SVF characterisation, it still remains unclear which intraoperative SVF isolation method is preferred. Future studies are recommended to implement standardised guidelines to standardise methods and improve comparability between studies.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"1008-1021"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between venous leg ulcers and knee osteoarthritis: A Mendelian randomization study. 腿部静脉溃疡与膝关节骨性关节炎之间的关系：孟德尔随机研究

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-10-23 DOI: 10.1111/wrr.13229

Deyi Pan, Runqiao Yang, Yalan Zhang, Yuemei Chen, Yuhui Wang, Shixiong Xu

{"title":"Association between venous leg ulcers and knee osteoarthritis: A Mendelian randomization study.","authors":"Deyi Pan, Runqiao Yang, Yalan Zhang, Yuemei Chen, Yuhui Wang, Shixiong Xu","doi":"10.1111/wrr.13229","DOIUrl":"10.1111/wrr.13229","url":null,"abstract":"To gain a clearer understanding of the relationship between venous leg ulcers (VLUs) and knee osteoarthritis (KOA), we performed a two-sample, bidirectional Mendelian randomization (MR) analysis in this study. The present MR was carried out using summary data from publicly available genome-wide association studies. After filtering single-nucleotide polymorphism (SNP), we applied a variety of MR methods including inverse variance weighted (IVW), MR egger, weighted mode, and weighted median. IVW analysis revealed that the genetic association between VLUs and KOA was not significant (β = -0.017; SE 0.039; p = 0.658). In agreement with the IVW analysis, the findings of the weighted median estimator (β = -0.017; SE 0.052, p = 0.751), MR egger (β = 0.057; SE 0.084; p = 0.513), and weighted mode (β = 0.060; SE 0.078; p = 0.456) indicated the absence of a significant genetic association between VLUs and KOA. Furthermore, reverse causality analysis suggested a lack of genetic relationship between KOA and VLUs. In conclusion, the present MR study does not suggest a causal relationship or reverse causal relationship between VLUs and KOA.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"895-903"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstracts Oral Presentations ETRS-WHS-SKINTERM Congress 2024 in Amersfoort. 2024 年阿默斯福特 ETRS-WHS-SKINTERM 大会口头报告摘要。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 DOI: 10.1111/wrr.13225

引用次数: 0

Outcomes of dermal substitutes in burns and burn scar reconstruction: A systematic review and meta-analysis. 皮肤替代物在烧伤和烧伤疤痕重建中的疗效：系统回顾和荟萃分析。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-10-22 DOI: 10.1111/wrr.13226

Anna S van den Bosch, Robin A F Verwilligen, Anouk Pijpe, Eelke Bosma, Cees H van der Vlies, Ymke Lucas, George L Burchell, Paul P M van Zuijlen, Esther Middelkoop

{"title":"Outcomes of dermal substitutes in burns and burn scar reconstruction: A systematic review and meta-analysis.","authors":"Anna S van den Bosch, Robin A F Verwilligen, Anouk Pijpe, Eelke Bosma, Cees H van der Vlies, Ymke Lucas, George L Burchell, Paul P M van Zuijlen, Esther Middelkoop","doi":"10.1111/wrr.13226","DOIUrl":"10.1111/wrr.13226","url":null,"abstract":"Dermal substitutes have been introduced in burn care to improve wound healing outcomes; however, their use remains limited in standard treatments. This systematic review and meta-analysis aimed to evaluate the outcomes of dermal substitutes in patients with burns and patients requiring burn scar reconstruction and subsequently contribute to optimising the integration of dermal substitutes into clinical practice and reducing the knowledge gap. A comprehensive search across various databases included human studies from peer-reviewed journals on dermal substitutes for deep dermal and full-thickness burns, and scar reconstruction across all ages. Data from comparative trials were extracted, focusing on patient and wound characteristics, treatment specifics, and outcomes related to wound healing and scar quality. Meta-analysis was performed on trials reporting similar post-burn measures, with statistical heterogeneity assessed. Outcomes were presented using mean differences or odds ratios with 95% confidence intervals. A total of 31 comparative trials were included. The overall quality of the studies was considered moderate. The meta-analysis indicated delayed re-epithelialization 4-7 days after treatment with a collagen-elastin matrix compared to split-thickness skin graft in acute burns (-7.30%, p = 0.02). Significant improvement in subjective scar quality was observed with acellular dermal matrix compared to split-thickness skin graft in acute burn wounds 6 months post-operative (-1.95, p <0.01). While acknowledging the initially delayed wound healing, incorporating dermal substitutes into the surgical treatment of burn patients holds promise for enhancing scar quality. However, future research must prioritise outcome measure uniformity, address variations in dermal substitute application, and standardise indications for consistent and effective practices.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"960-978"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenol hydroxytyrosol present olive oil improves skin wound healing of diabetic mice. 橄榄油中的多酚羟基酪醇能改善糖尿病小鼠皮肤伤口的愈合。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-11-01 Epub Date: 2024-09-03 DOI: 10.1111/wrr.13217

Matheus Silva Duarte, Victor Müller Fuhro, Jeane de Souza Nogueira, Bruna Romana-Souza

{"title":"Polyphenol hydroxytyrosol present olive oil improves skin wound healing of diabetic mice.","authors":"Matheus Silva Duarte, Victor Müller Fuhro, Jeane de Souza Nogueira, Bruna Romana-Souza","doi":"10.1111/wrr.13217","DOIUrl":"10.1111/wrr.13217","url":null,"abstract":"The imbalance in oxidant production and chronic inflammation are the main mechanisms that lead to the detrimental effects of diabetes on skin wound healing. Thus, administration of antioxidants could improve diabetic wound healing. This study aimed to understand the effects of extra virgin olive oil (EVOO) or hydroxytyrosol (HT) in skin wound healing under diabetic conditions. Skin wounds in streptozotocin-induced diabetic mice were topically treated with HT. Some diabetic animals were fed with a diet rich in EVOO. Wounds were harvested 7 days later. In in vitro assays, fibroblasts and macrophages were treated with high levels of glucose and HT. The EVOO or HT promoted wound closure and collagen deposition in diabetic mouse wounds. The EVOO or HT reduced the number of infiltrated neutrophils, tumour necrosis factor-α, lipid peroxidation, and nuclear factor erythroid 2-related factor 2 in diabetic mouse wounds. The EVOO or HT also increased the number of macrophages with anti-inflammatory phenotype and interleukin-10 in diabetic mouse wounds. In the in vitro assays, HT promoted the fibroblast migration, collagen gel contraction, and switched macrophages to an anti-inflammatory phenotype under high glucose conditions. In conclusion, the diet supplementation with EVOO or topical application of HT promotes skin wound healing under diabetic conditions and can be a possible therapeutic tool for the treatment of those lesions.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"904-915"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0