Wound Repair and Regeneration最新文献_第4页

Topical Tranexamic Acid, Adrenaline and Bupivacaine Solution for Pain Management and Healing in Split-Thickness Skin Graft Donor Sites: An Open-Label Interventional Study With Randomised Side Allocation. 局部氨甲环酸、肾上腺素和布比卡因溶液用于裂厚皮肤移植供体疼痛管理和愈合：一项随机侧分配的开放标签介入研究。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70060

Pattana Ongkasuwan

{"title":"Topical Tranexamic Acid, Adrenaline and Bupivacaine Solution for Pain Management and Healing in Split-Thickness Skin Graft Donor Sites: An Open-Label Interventional Study With Randomised Side Allocation.","authors":"Pattana Ongkasuwan","doi":"10.1111/wrr.70060","DOIUrl":"10.1111/wrr.70060","url":null,"abstract":"Split-thickness skin graft donor site wounds present significant challenges in pain management and healing optimization. This intra-individual comparative study evaluated the efficacy and safety of a novel topical solution containing tranexamic acid, adrenaline and bupivacaine versus standard paraffin-chlorhexidine dressings, with side allocation determined by computer randomisation after graft harvesting. Twelve patients received standardised solution application on one donor site and standard treatment on the contralateral site, with each side's dressing changes performed according to protocol. The treatment group demonstrated significantly lower mean pain scores across all time intervals (1.1 vs. 5.3 at 24 h, p < 0.001). Mean epithelialization rates at Days 10-14 were higher in the treatment group (97.1% vs. 94.8%, p < 0.05), with faster time to complete healing (median 12 vs. 16 days, p = 0.002). No significant hemodynamic changes occurred following solution application, with only one case of transient tachycardia reported. Vancouver Scar Scale scores at eight weeks showed a trend favouring the treatment (3.8 vs. 4.2, p = 0.15), although this difference was not statistically significant. No infections were observed in either group. These findings suggest that this novel topical solution with transparent film dressing effectively reduces pain and accelerates healing in donor site wounds without compromising safety, providing a promising new option for managing these challenging surgical wounds.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70060"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Negative Pressure Wound Therapy With Instillation and Dwell in Removing Nonviable Tissue, Promoting Granulation Tissue, and Reducing Surgical Debridements: A Systematic Literature Review. 负压伤口灌注和留置治疗在去除不存活组织、促进肉芽组织和减少手术清创方面的有效性：系统的文献综述。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70059

Julie Acosta, Lydia Galarza, Margaret Marsh, Ricardo R Martinez, Mark Eells, Ashley W Collinsworth

{"title":"Effectiveness of Negative Pressure Wound Therapy With Instillation and Dwell in Removing Nonviable Tissue, Promoting Granulation Tissue, and Reducing Surgical Debridements: A Systematic Literature Review.","authors":"Julie Acosta, Lydia Galarza, Margaret Marsh, Ricardo R Martinez, Mark Eells, Ashley W Collinsworth","doi":"10.1111/wrr.70059","DOIUrl":"10.1111/wrr.70059","url":null,"abstract":"Surgical debridement is a common treatment for complex wounds but can present risks for patients. Negative pressure wound therapy with instillation and dwell (NPWTi-d) using reticulated open cell foam dressings with 1 cm holes (ROCF-CC) provides hydromechanical wound cleaning and preparation and can be applied outside the operating room at the bedside. This systematic literature review examined the effectiveness of NPWTi-d with ROCF-CC in removing nonviable tissue and infectious material, promoting granulation tissue, and reducing surgical debridements. A systematic search was conducted utilising PubMed, Embase, and ClinicalTrials.gov to identify studies conducted from 1 January 2015-31 August 2022. Study outcomes related to nonviable tissue, granulation tissue, and debridement were summarised and analysed using descriptive statistics. Twenty-one studies including 178 patients who received NPWTi-d with ROCF-CC were included. Evidence of reduction in necrotic and infected tissue following treatment was observed in 97.9% of wounds across 17 studies. Formation of granulation tissue after NPWTi-d with ROCF-CC was reported in 99.2% of wounds across 14 studies. Over 63% of patients avoided surgical debridements in 8 studies, and a statistically significant decrease in surgical debridements was noted in 2 comparative studies. This systematic review provides real-world evidence demonstrating the effectiveness of NPWTi-d with ROCF-CC in the hydromechanical removal of infectious materials, non-viable tissue, and wound debris; reduction of surgical debridements; and promotion of granulation tissue. Thus, NPWTi-d with ROCF-CC may potentially reduce or eliminate the need for surgical debridement by removing non-viable tissue through hydromechanical action.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70059"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12207569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibroblast-Derived TGFβ1 Regulates Skin Repair and Fibrosis. 成纤维细胞来源的tgf - β1调节皮肤修复和纤维化。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70065

Sebastian Willenborg, Katrin Schönborn, Mugdha Sawant, Anna Bornikoel, Takumi Yamane, Isabel Zeinert, Beate Eckes, Sabine A Eming, Thomas Krieg

{"title":"Fibroblast-Derived TGFβ1 Regulates Skin Repair and Fibrosis.","authors":"Sebastian Willenborg, Katrin Schönborn, Mugdha Sawant, Anna Bornikoel, Takumi Yamane, Isabel Zeinert, Beate Eckes, Sabine A Eming, Thomas Krieg","doi":"10.1111/wrr.70065","DOIUrl":"10.1111/wrr.70065","url":null,"abstract":"Activation of fibroblasts and formation of myofibroblasts are essential for granulation tissue formation following injury. In fibrotic reactions, excessive deposition of ECM by the activated fibroblasts determines scar formation and functional failure. Although these events critically depend on the activity of a plethora of growth factors and cytokines, TGFβ1 is a unique player controlling the immune response and proliferation of many cell types. Different cell types contribute to its release and activation, which is also regulated by the interaction with the ECM and by mechanical forces. The aim of this study was to elaborate whether fibroblast-derived TGFβ1 plays a critical role during these processes. The data demonstrate a dynamic expression of TGFβ1 during tissue repair. Cell-specific ablation of Tgfb1 in fibroblasts revealed that deletion of TGFβ1 attenuates bleomycin-induced skin fibrosis and perturbs maturation of granulation tissue in skin wounds. Absence of fibroblast-derived TGFβ1 induced vascular alterations (less vascular density and branching, haemorrhage) in early wound healing. This was associated with alterations in the formation of stable ECM structure. This can be explained by paracrine regulation of endothelial cells or pericytes by fibroblast-released TGFβ1 and by impaired expression of pro-angiogenic factors in TGFβ1-deficient fibroblasts. Our findings provide novel mechanistic insights into the central role of fibroblast-derived TGFβ1 for early stages of tissue repair and fibrosis in the skin.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70065"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shotgun Metagenomic Investigation of the Microbiome in Diabetic Foot Infections Compared to Healthy Skin. 与健康皮肤相比，糖尿病足部感染微生物组的散弹枪宏基因组研究

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70074

Nerdjes Ferhaoui, Mohammed Sebaihia, Tsuyoshi Sekizuka, Makoto Kuroda

{"title":"Shotgun Metagenomic Investigation of the Microbiome in Diabetic Foot Infections Compared to Healthy Skin.","authors":"Nerdjes Ferhaoui, Mohammed Sebaihia, Tsuyoshi Sekizuka, Makoto Kuroda","doi":"10.1111/wrr.70074","DOIUrl":"10.1111/wrr.70074","url":null,"abstract":"Diabetic foot infection (DFI) is a major complication of diabetes, causing significant morbidity and mortality. Host factors and microorganisms in DFI can disrupt healing processes, leading to chronic, non-healing wounds. The aim of this study was to characterise the microbiome of DFIs and contralateral healthy foot skin (CHFS). Thirty-two diabetic patients were enrolled in this study. Samples were obtained from DFIs and CHFS from the same patient. The microbiome was profiled using metagenomic shotgun sequencing. All the samples were polymicrobial, with a predominance of the obligate anaerobes belonging to Bacteroidetes in PEDIS 4. While PEDIS 3 and 2 were dominated by Proteobacteria. CHFS showed similar bacterial composition across all grades of severity, and the most abundant genera detected were Corynebacterium, Staphylococcus, Pseudomonas, and Cutibacterium. The CHFS was more diverse than DFIs in PEDIS 3 and 4. However, DFIs and CHFS in PEDIS 2 present similar diversity. In addition, DFIs of this grade exhibited a high proportion of Corynebacterium as well as CHFS. PCoA analysis demonstrated that the community structure of DFIs was different from that of CHFS, with Prevotella, Bacteroides, and Porphyromonas the main contributors to the clustering. Neighbour-Net analyses revealed that DFIs exhibited lower diversity compared to CHFS and harboured a more homogeneous dominant bacterial community. Our study revealed a high abundance of obligate anaerobes, including Bacteroides, Prevotella, Morganella, and Porphyromonas, in more severe infections; along with a decrease in microbial diversity. Additionally, there was a decrease in the abundance of key bacteria from the normal skin microbiota.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70074"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patient Race and Ethnicity Do Not Predict Ulceration Among Ambulatory Patients With Venous Insufficiency. 患者种族和民族不能预测静脉功能不全的门诊患者溃疡的发生。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70057

Andrew Nicholas, Fernando J Salinas, Tammy Gonzalez, Katherine Baquerizo Nole, Robert S Kirsner, Alan B Fleischer

{"title":"Patient Race and Ethnicity Do Not Predict Ulceration Among Ambulatory Patients With Venous Insufficiency.","authors":"Andrew Nicholas, Fernando J Salinas, Tammy Gonzalez, Katherine Baquerizo Nole, Robert S Kirsner, Alan B Fleischer","doi":"10.1111/wrr.70057","DOIUrl":"10.1111/wrr.70057","url":null,"abstract":"Venous ulcers are among the most common chronic wounds, considerably impacting quality of life and causing substantial economic burden. This study aimed to determine if race and ethnicity are predictors for ulceration among ambulatory patients with venous insufficiency. Physician-reported data were extracted from the National Ambulatory Medical Care Survey (NAMCS) collected between 2014 and 2019. An estimated 42.7 (95% Confidence Interval (CI) 39.9-45.5) million outpatient visits with a diagnosis of venous insufficiency, unspecified chronic wound, or varicose veins were included in the analysis. Patient race and ethnicity were not associated with differences in the likelihood of ulceration. However, venous ulceration was associated with the male sex (Adjusted Odds Ratio (aOR) 2.5; 95% CI 1.2-5.2, p = 0.02) and was more likely among visits with surgical specialties (aOR 5.2; 95% CI 2.1-13.4, p = 0.0005). While prior studies report greater chronic wound treatment rates among non-White racial minority patients, these findings do not demonstrate differences in ambulatory care for venous ulceration within nationally representative data.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70057"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12223914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re: Antimicrobial Potential of Maggot Excretions/Secretions From Blowflies (Diptera: Calliphoridae). 回复：蝇蛆排泄物/分泌物的抗菌潜力（双翅目：蝇科）。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70083

Nathkapach Kaewpitoon Rattanapitoon, Nav La, Schawanya Kaewpitoon Rattanapitoon

引用次数: 0

The Role of Glutamine and Arginine in Wound Healing of Pressure Ulcers: A Systematic Review. 谷氨酰胺和精氨酸在压疮创面愈合中的作用：系统综述。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70077

Tim Torsy, Inge Tency, Dimitri Beeckman, Kirsi Isoherranen, Mary Litchford, Flore De Vylder

{"title":"The Role of Glutamine and Arginine in Wound Healing of Pressure Ulcers: A Systematic Review.","authors":"Tim Torsy, Inge Tency, Dimitri Beeckman, Kirsi Isoherranen, Mary Litchford, Flore De Vylder","doi":"10.1111/wrr.70077","DOIUrl":"10.1111/wrr.70077","url":null,"abstract":"Pressure ulcers pose a significant health challenge, requiring effective management strategies. Nutrition, particularly arginine and glutamine, supports collagen synthesis and tissue repair. This review evaluates the role of enteral glutamine and arginine supplementation on wound healing outcomes, addressing gaps in previous research. A PRISMA-guided systematic search of five databases identified studies published between 2004 and 2024 on adults with pressure ulcers receiving these supplements. Outcomes assessed included healing time, wound size reduction, local infection, recurrence, and pain. A narrative synthesis was performed due to heterogeneity, with bias assessed via Cochrane RoB2 and JBI checklists. Fifteen studies involving 1085 participants were included. Findings indicated a trend toward improved healing with arginine or combined arginine/glutamine supplements, with relative wound size reductions of 18.6% to 98.2% over 2 to 20 weeks. However, inconsistencies were noted, with seven studies showing non-significant or unreported differences in wound size, and six studies with similar issues for healing time. Glutamine was examined only in combination with arginine, limiting insights into its isolated effects. None of the studies reported on recurrence or pain outcomes. While arginine shows potential for enhancing healing, evidence remains inconclusive. Future research should emphasise follow-up until complete wound closure and explore the independent effects of glutamine on wound healing outcomes.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70077"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wound Bed Temperature has Potential to Indicate Infection Status: A Cross-Sectional Study. 伤口床温度有可能指示感染状态：一项横断面研究。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70072

Adam R Collins, Gerard M O'Connor, Darragh A Ryan, Molly Parmeter, Sean Dinneen, Georgina Gethin

{"title":"Wound Bed Temperature has Potential to Indicate Infection Status: A Cross-Sectional Study.","authors":"Adam R Collins, Gerard M O'Connor, Darragh A Ryan, Molly Parmeter, Sean Dinneen, Georgina Gethin","doi":"10.1111/wrr.70072","DOIUrl":"10.1111/wrr.70072","url":null,"abstract":"This study investigates the potential of wound bed temperature, measured using an IR camera, to aid in the clinical assessment of chronic wounds. The study captured thermal images from 267 patients with chronic wounds (diabetic foot ulcers, pressure ulcers, venous leg ulcers and arterial ulcers) with corresponding photographic images and clinical data. Temperature measurements were extracted from thermal images, focusing on both the centre of the wound and the surrounding periwound skin. Statistical analyses were employed to evaluate the relationship between wound temperature distribution and clinical diagnosis. The results showed a strong correlation between wound centre temperature and the average temperature across the entire wound (R2 = 0.977). This indicates that a single-point measurement is representative of the entire wound, simplifying wound temperature assessment. A fair correlation was found between the temperature difference between the wound and periwound and the clinician's assessment of infection status (Pearson coefficient = 0.32). The study concludes that thermal imaging holds promise as a supplementary tool for clinicians in assessing chronic wound status, especially in cases where infection is unclear. It is a low-cost, non-contact, and easy-to-use technique.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70072"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12315627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Inflammatory Response in Surgical Wound Drainage Fluid in Scoliosis Surgery: A Study of Neuromuscular vs. Idiopathic Patients. 脊柱侧凸手术中伤口引流液炎症反应的比较分析：神经肌肉与特发性患者的研究。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70076

Meredith J Crane, Robin L McKinney, Alexander R D Jordon, Craig P Eberson, Amanda M Jamieson

{"title":"Comparative Analysis of Inflammatory Response in Surgical Wound Drainage Fluid in Scoliosis Surgery: A Study of Neuromuscular vs. Idiopathic Patients.","authors":"Meredith J Crane, Robin L McKinney, Alexander R D Jordon, Craig P Eberson, Amanda M Jamieson","doi":"10.1111/wrr.70076","DOIUrl":"10.1111/wrr.70076","url":null,"abstract":"This study examines immune and inflammatory responses in draining wound fluid over the course of the early stages of wound healing in patients recovering from spinal fusion surgery. The inflammatory phase of wound healing is essential for setting the stage for successful tissue repair and preventing chronic or poorly healing wounds. Scoliosis can be idiopathic or occur secondary to neuromuscular disorders, which are known to be associated with poor wound healing outcomes. We hypothesised that neuromuscular scoliosis patients would exhibit differences in inflammatory wound healing markers compared to idiopathic scoliosis patients. Comparison of the cellular and cytokine contents of draining wound fluid revealed that several inflammatory cytokines were elevated in the neuromuscular scoliosis patient group compared to idiopathic, whereas the leukocyte contents were the same between groups. This study shows that draining wound fluid is a good source of cellular and soluble biomarkers for acute wound healing and can be used to determine changes in individuals at risk for wound healing complications.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70076"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Oxygen Manipulation on Myofibroblast Phenotypic Transformation in Patients With Radiation-Induced Fibrosis. 氧处理对放射性纤维化患者肌成纤维细胞表型转化的影响。

IF 3.4 3区医学

Wound Repair and Regeneration Pub Date : 2025-07-01 DOI: 10.1111/wrr.70075

Eric V Mastrolonardo, Sarah Sussman, Bo Yun, Victor Jegede, Dev R Amin, Joel Rosenbloom, Andrew P South, Voichita Bar-Ad, Peter J Wermuth, Adam J Luginbuhl

{"title":"Effects of Oxygen Manipulation on Myofibroblast Phenotypic Transformation in Patients With Radiation-Induced Fibrosis.","authors":"Eric V Mastrolonardo, Sarah Sussman, Bo Yun, Victor Jegede, Dev R Amin, Joel Rosenbloom, Andrew P South, Voichita Bar-Ad, Peter J Wermuth, Adam J Luginbuhl","doi":"10.1111/wrr.70075","DOIUrl":"10.1111/wrr.70075","url":null,"abstract":"We tested if hyperoxic conditions can reduce the proportion of active myofibroblasts, which are assumed to be a major driver of head and neck radiation-induced fibrosis, as measured by expression levels of pro-fibrotic genes. Radiated, non-cancerous soft tissue from the head and neck and skin/soft tissue from non-radiated flap donor site were collected from each patient. Myofibroblast density was quantified using immunofluorescence staining with α-SMA and DAPI and visualisation under confocal microscopy and compared between baseline non-radiated and radiated tissue from the same patient. From each tissue specimen, fibroblast cell lines were cultured and exposed to either normoxic, hypoxic, or hyperoxic conditions for 10 days. Total RNA was extracted and reverse-transcribed, and gene expression levels were quantified using RT-PCR. Relative gene expression levels of pro-fibrotic genes COL1A1, COL3A1, FN-EDA, α-SMA, HIF-1α, VEGFα, and VEGFR were compared between normoxic, hypoxic, and hyperoxic treatment groups. Three patients with six total tissue samples were acquired. Radiated tissue contained a higher density of myofibroblasts (calculated as cells/mm2) and demonstrated higher expression of pro-fibrotic genes than non-radiated donor site tissue. Hyperoxia decreases expression levels of pro-fibrotic genes in radiated and non-radiated tissue, while hypoxia increases pro-fibrotic gene expression levels in radiated and non-radiated tissue. Study findings indicate that hypoxia is a driver of myofibroblast activation and that subjects with radiation-induced fibrosis of the head and neck have increased expression of myofibroblastic phenotype. Hyperoxygenation can reduce the proportion of active myofibroblasts, revealing a potential therapeutic method to halt chronic fibrotic pathways.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70075"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12358765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0