Wound Repair and Regeneration最新文献

{"title":"Re-Epithelialisation in a Yorkshire Pig Full-Thickness Excisional Wound Model Is Associated With Keratinocyte Activation, Oxidative Stress, and Biomacromolecule Oxidation.","authors":"Dylan Tinney, John T Walker, Emily Truscott, Douglas W Hamilton","doi":"10.1111/wrr.70082","DOIUrl":"https://doi.org/10.1111/wrr.70082","url":null,"abstract":"<p><p>Pig skin represents the best analogue for human skin both anatomically and physiologically, with this model used extensively for pre-clinical testing of therapeutics and biomaterials. However, the molecular processes underlying re-epithelialisation in pigs are still not well described compared to murine models. Our objective was to characterise the re-epithelialisation process in porcine full-thickness excisional wounds in Yorkshire pigs. Immunohistochemistry markers for keratinocyte differentiation, activation and oxidative stress were used at 7 days and 28 days post-wounding, and in healthy control skin to characterise protein expression. We show at day 7, re-epithelialisation is associated with reduced cytokeratin 10, E-cadherin and filaggrin and an increase in cytokeratin 14, cytokeratin 16 and cytokeratin 17. At day 28, cytokeratin 16 remained expressed, but cytokeratin 14 only associated with basal keratinocytes and cytokeratin 10 with suprabasal keratinocyte layers. At day 7, both phospho-nuclear factor kappa B and the antioxidant transcription factor nuclear factor-erythroid 2-related factor 2 show nuclear translocation at the wound edge, which is attenuated by day 28. Concomitant with these observations, we show that re-epithelialisation is associated with guanosine oxidation, protein nitration, and lipid peroxidation at both day 7 and 28. Our observations confirm the baseline expression profile of keratinocytes during normal healing of full-thickness excisional wounds in Yorkshire pigs. Characterisation of similar markers in human healing will improve our understanding of the validity of the Yorkshire pig model for use in the testing of therapeutics for impaired skin healing in humans.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70082"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traumatic Brain Injury Induces Early Barrier Protective Responses in Incisional Skin Wounds Accelerating Cutaneous Wound Healing.","authors":"Mahyar Aghapour, Florian Olde Heuvel, Albrecht Fröhlich, Adelheid Heinzl, Pallab Maity, Karmveer Singh, Yongfang Wang, Jinnan Cheng, Francesco Roselli, Karin Scharffetter-Kochanek","doi":"10.1111/wrr.70079","DOIUrl":"https://doi.org/10.1111/wrr.70079","url":null,"abstract":"<p><p>Though Traumatic Brain Injury (TBI) and skin trauma often occur together, it is unresolved whether TBI changes the healing of skin wounds. We here explored whether TBI impacts the sequence of events during skin wound healing. Incisional skin wounds from mice subjected to TBI were assessed employing unbiased transcriptome analysis and immunostaining. Transcriptome analysis at day 1 after combined trauma detects a significant enrichment of genes involved in macrophage and T cell recruitment and activation in contrast to skin wounds without TBI. At day 7 after combined trauma, genes in pathways of re-epithelialisation including cornification and keratinisation and of anti-inflammatory responses were highly enriched. These findings were confirmed by immunostaining with increased re-epithelialisation and cornification and an increased number of macrophages and T cells resolving inflammation. Moreover, the number of dermal myofibroblasts is highly increased in skin wounds after combined trauma. Collectively, TBI induces a robust defence response characterised by early onset of enhanced immunity, faster epidermal barrier formation, and myofibroblast-driven acceleration of wound closure, which may together help counteract systemic infection.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70079"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12395893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re: Early Patient-Reported Outcomes as Predictors of Long-Term Scar Satisfaction: An Exploratory Cohort Study.","authors":"Schawanya K Rattanapitoon, Patpicha Arunsan, Chutharat Thanchonnang, Nathkapach K Rattanapitoon","doi":"10.1111/wrr.70101","DOIUrl":"https://doi.org/10.1111/wrr.70101","url":null,"abstract":"","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70101"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commentary on \"Patient Race and Ethnicity Do Not Predict Ulceration Among Ambulatory Patients With Venous Insufficiency\".","authors":"Mostafa Javanian, Amirhossein Zohrehvand, Mohammad Barary, Farhad Bagherian, Soheil Ebrahimpour","doi":"10.1111/wrr.70086","DOIUrl":"https://doi.org/10.1111/wrr.70086","url":null,"abstract":"","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70086"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Wound to Hair Follicle: Unravelling the Mechanisms and Molecular Networks in Wound-Induced Hair Follicle Neogenesis.","authors":"Mingwei Shang, Ziyuan Tang, Weining Huang, Zhongrong Liu","doi":"10.1111/wrr.70093","DOIUrl":"https://doi.org/10.1111/wrr.70093","url":null,"abstract":"<p><p>Wound-induced hair follicle neogenesis (WIHN), a regenerative process observed in select mammals, involves the formation of new hair follicles following skin injury. Although exhibiting similarities to embryonic hair follicle development, WIHN also presents notable distinctions. Despite considerable efforts to elucidate its underlying mechanisms, several aspects of WIHN remain unclear. A comprehensive understanding of the factors and signalling pathways governing this process holds promise for developing novel therapeutic interventions targeting hairless scar formation in humans.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70093"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Patient-Reported Outcomes as Predictors of Long-Term Scar Satisfaction: An Exploratory Cohort Study.","authors":"Antoinette Nguyen, Rena Li, Robert Galiano","doi":"10.1111/wrr.70094","DOIUrl":"10.1111/wrr.70094","url":null,"abstract":"<p><p>Early prediction of long-term scar outcomes is essential for guiding clinical decision-making and improving patient satisfaction. This study investigates whether 3-month patient-reported outcomes (PROs) using SCAR-Q domains-psychosocial, appearance and quality of life (QoL)-predict 12-month outcomes, and how these relate to objective scar measures. A prospective cohort of 20 female patients undergoing various surgical procedures completed SCAR-Q and Patient and Observer Scar Assessment Scale (POSAS) evaluations at 3 and 12 months postoperatively. Correlation and linear regression analyses assessed associations and predictive validity between early and late scar outcomes. SCAR-Q QoL scores demonstrated strong predictive validity (R² = 0.49, p < 0.001; ρ = 0.70, p < 0.001), whereas psychosocial and appearance domains showed weak, nonsignificant associations (R² = 0.12 and 0.10, respectively; p > 0.1). Objective scar characteristics-particularly width and height-were significantly correlated with poorer 12-month appearance and psychosocial scores (e.g., ρ = -0.743 for height vs. appearance, p < 0.001; ρ = -0.605 for width vs. psychosocial, p = 0.0047). In point-biserial correlations, wider and taller scars at 3 months were more likely to be rated as 'bad' at 12 months (r ≥ |0.53|, p ≤ 0.016). POSAS and overall opinion scores also significantly improved over time (p < 0.05), but some patients reported increased appearance-related distress despite objective improvements. In conclusion, early QoL assessments reliably predict long-term outcomes, while appearance and psychosocial perceptions may shift over time. These findings support routine use of PROs in early postoperative care to inform personalised interventions and optimise scar management.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70094"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish Acellular Dermal Matrix Promotes Repair of Full-Thickness Skin Defects in Mice and Bama Pigs.","authors":"Zi-Yi Wang, Zi-Hao Lin, Ruo-Tao Liu, Zhe Liu, Hao Peng, Zhi-Chao Hu, Wei-Qing Fu, Li-Ming Jin, Chang-Qing Zhang, Qian Tang, Zhen-Zhong Zhu, Xiao-Juan Wei","doi":"10.1111/wrr.70091","DOIUrl":"10.1111/wrr.70091","url":null,"abstract":"<p><p>This study aimed to develop an acellular dermal matrix derived from tilapia skin and evaluate its potential as a bioscaffold for skin wound repair. Structural and compositional changes before and after decellularisation were assessed through histological staining, electron microscopy and immunological analysis. The matrix exhibited low immunogenicity, preserved extracellular matrix architecture and retained key bioactive components. In vitro, the matrix significantly promoted cell proliferation, migration, and tube formation in human umbilical vein endothelial cells and human foreskin fibroblasts. In vivo, full-thickness skin defect models in Balb/c mice and Bama pigs demonstrated that the tilapia-derived matrix not only accelerated wound closure but also improved the quality of tissue regeneration by enhancing collagen deposition and vascularisation. Compared to the commercial porcine-derived matrix, the fish-derived scaffold exhibited superior regenerative outcomes. Notably, transcriptomic profiling of wound tissue revealed that the matrix modulated a range of biological pathways, including immune regulation, extracellular matrix remodelling and angiogenesis, indicating a multifaceted interaction between the biomaterial and host tissue. These findings underscore the excellent biocompatibility and therapeutic efficacy of the tilapia-derived matrix, supporting its potential as a safe, economical and sustainable bioscaffold for clinical skin repair. The inclusion of a large animal model provides critical translational relevance due to the anatomical and physiological similarity between porcine and human skin, while transcriptomic analysis offers valuable mechanistic insights into matrix-tissue interactions.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70091"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"H₂O₂-Producing Electrochemical Bandages Are Active Using Off-the-Shelf Hydrogels.","authors":"Eda Dagsuyu, Paige Kies, Robin Patel, Haluk Beyenal","doi":"10.1111/wrr.70092","DOIUrl":"10.1111/wrr.70092","url":null,"abstract":"<p><p>An electrochemical bandage (e-bandage) that generates hydrogen peroxide (H₂O₂) through a combination of working, counter and reference electrodes used with an electrolyte-providing hydrogel is being developed for wound infection management. e-Bandage biocidal activity was previously demonstrated using Xanthan gum hydrogel. For clinical use, clinically used hydrogels would be ideal, but their use with the described e-bandage has not been shown. The goal of this work was to evaluate the biocidal activity of off-the-shelf, clinically used hydrogels when used with a 1.77 cm² H₂O₂-producing e-bandage. e-Bandage electrochemical parameters and activity against methicillin-resistant Staphylococcus aureus IDRL-6169 and Acinetobacter baumannii ATCC-17978 biofilms were assessed with six off-the-shelf hydrogels. Variations in hydrogel composition affected electrochemical parameters, which was associated with differences in biocidal activity. Results of this study inform the selection of off-the-shelf hydrogels for use with H₂O₂-producing e-bandages.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70092"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12438947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired Provisional Matrix Stimulates Regenerative Healing of Diabetic Wounds.","authors":"Walker D Short, Phillip A Kogan, Nava P Rijal, Aditya Kaul, Benjamin W Padon, Carlos Zgheib, Hongkwan Cho, Bradley A Herbig, Bradley A King, Timothy M Crombleholme, Swathi Balaji, Daria A Narmoneva","doi":"10.1111/wrr.70088","DOIUrl":"10.1111/wrr.70088","url":null,"abstract":"<p><p>This study tested the hypothesis that diabetic wound treatment with biomimetic pro-angiogenic, proteolytically and mechanically stable RADA16-II peptide nanofibers promotes regenerative wound healing via attenuation of inflammation and stimulation of neovascularization. Two full-thickness excisional dorsal skin wounds were created on 8-10 week old female db/db mice and treated with nanofiber hydrogel or saline (control). Animals were euthanized on days 7, 14, 28, and 56 and their wounds were analysed for morphology, vascularization, strength, and inflammation. We observed that in situ treatment of db/db mouse wounds with nanofiber hydrogel resulted in regenerative healing, indicated by the increased presence of elastin fibrils, restored biomechanical properties, and reestablishment of a mature epidermis complete with basal, suprabasal, and stratified layers compared to saline-treated wounds. Additionally, wounds treated with nanofiber hydrogel exhibited enhanced neovascularization, increased expression of anti-inflammatory cytokine interleukin-10, reduced expression of inflammation markers and transforming growth factor-β1 and -β2, as well as decreased myofibroblast counts. Overall, this novel drug-free approach enables accelerated diabetic wound healing by shifting inflammatory and pro-fibrotic cytokine balance towards factors associated with neovascularization-driven regenerative healing in the wound microenvironment. Our results demonstrate that in situ manipulation of the wound microenvironment using bio-mimetic peptide NF matrix may be a promising strategy for faster and more durable wound closure to improve healing of chronic wounds.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70088"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Wound Care With 3-D Imaging: Clinical Applications, Performance and Future Directions.","authors":"Adriano Antonio Mehl, Nicole Abdullah, Paula Karina Hembecker, Mauren Abreu de Souza","doi":"10.1111/wrr.70089","DOIUrl":"https://doi.org/10.1111/wrr.70089","url":null,"abstract":"<p><p>Chronic wounds pose a growing global health challenge. Accurate assessment is essential for monitoring healing, yet traditional two-dimensional methods lack volumetric analysis. Emerging three-dimensional imaging technologies offer enhanced precision, but their clinical validation and prognostic utility remain unclear. This scoping review aimed to map and synthesise the available evidence on the clinical application and predictive potential of three-dimensional wound assessment technologies in adults with chronic wounds. A systematic search was performed across five electronic databases-MEDLINE via PubMed, EMBASE, Web of Science, CINAHL, and IEEE Xplore-to identify peer-reviewed studies published between 2014 and 2024. Eligible studies included original research evaluating the clinical application, reliability, accuracy, or predictive performance of three-dimensional wound assessment technologies. Two independent reviewers performed title/abstract screening, full-text eligibility assessment, and data extraction. Data were synthesised narratively, with emphasis on methodological quality, clinical applicability, and standardisation practices across technologies. Eleven studies were included, encompassing 734 wounds and 307 patients. Technologies evaluated included optical coherence tomography, structured-light scanners, stereophotogrammetry, and smartphone-based structure-from-motion systems. Most platforms demonstrated high reproducibility for surface area measurement (ICC > 0.95) and moderate accuracy for depth and volume. Methodological heterogeneity and limited standardisation of validation protocols hindered cross-platform comparability. No study integrated volumetric metrics into predictive healing models. Three-dimensional wound assessment technologies show strong potential to enhance wound measurement precision, documentation, and non-invasive monitoring. Robust validation frameworks and integration of volumetric metrics into predictive models are needed to realise their clinical value in precision wound care.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 5","pages":"e70089"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}