Wound Repair and Regeneration最新文献_第10页

Comparison of dermal and eschar fibroblasts in full skin equivalents. 真皮成纤维细胞与全皮肤等效成纤维细胞的比较。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.70001

Gizem Coşar Kutluoğlu, Marcel Vlig, Anouk Elgersma, Bouke K H L Boekema, Willeke F Daamen, Claudia Doberenz, Dominique Manikowski

{"title":"Comparison of dermal and eschar fibroblasts in full skin equivalents.","authors":"Gizem Coşar Kutluoğlu, Marcel Vlig, Anouk Elgersma, Bouke K H L Boekema, Willeke F Daamen, Claudia Doberenz, Dominique Manikowski","doi":"10.1111/wrr.70001","DOIUrl":"10.1111/wrr.70001","url":null,"abstract":"Full-thickness burn wounds pose significant problems, demanding specialised therapies to avoid complications and promote recovery. Eschar tissue, which forms in response to severe burns, contains viable fibroblasts, which migrate from the surrounding tissue in response to burn injury and exhibit a myofibroblast phenotype. The goal of this study was to characterise eschar-derived fibroblasts and examine their use for engineered in vitro full skin equivalents in comparison to normal dermal fibroblasts, which were harvested from non-injured skin. Microarray analysis indicated that eschar fibroblasts differ from dermal fibroblasts in various biological processes including inflammation, extracellular matrix formation, cell migration and differentiation. Skin equivalents with eschar fibroblasts showed similarities to those generated using normal dermal fibroblasts in terms of epidermis and dermis formation. However, in contrast to dermal fibroblast-based full skin equivalents, eschar fibroblast-based equivalents exhibited macroscopic contractile behaviour. In addition, eschar fibroblasts-based equivalents demonstrated higher alpha-smooth muscle actin expression on mRNA and protein levels. In conclusion, our findings suggest that eschar fibroblasts-based full skin equivalents hold promise as a platform to study burn wound environments as eschar fibroblasts are clinically more relevant fibroblasts and able to mimic certain aspects of the challenging wound environment in vitro.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e70001"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in bioactive wound dressings. 生物活性伤口敷料的最新进展。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 Epub Date: 2024-11-14 DOI: 10.1111/wrr.13233

Md Golam Nur, Mustafijur Rahman, Tanvir Mahady Dip, Md Hasibul Hossain, Nusrat Binta Hossain, Sara Baratchi, Rajiv Padhye, Shadi Houshyar

{"title":"Recent advances in bioactive wound dressings.","authors":"Md Golam Nur, Mustafijur Rahman, Tanvir Mahady Dip, Md Hasibul Hossain, Nusrat Binta Hossain, Sara Baratchi, Rajiv Padhye, Shadi Houshyar","doi":"10.1111/wrr.13233","DOIUrl":"10.1111/wrr.13233","url":null,"abstract":"Traditional wound dressings, despite their widespread use, face limitations, such as poor infection control and insufficient healing promotion. To address these challenges, bioactive materials have emerged as a promising solution in wound care. This comprehensive review explores the latest developments in wound healing technologies, starting with an overview of the importance of effective wound management, emphasising the need for advanced bioactive wound dressings. The review further explores various bioactive materials, defining their characteristics. It covers a wide range of natural and synthetic biopolymers used to develop bioactive wound dressings. Next, the paper discusses the incorporation of bioactive agents into wound dressings, including antimicrobial and anti-inflammatory agents, alongside regenerative components like growth factors, platelet-rich plasma, platelet-rich fibrin and stem cells. The review also covers fabrication techniques for bioactive wound dressings, highlighting techniques like electrospinning, which facilitated the production of nanofibre-based dressings with controlled porosity, the sol-gel method for developing bioactive glass-based dressings, and 3D bioprinting for customised, patient-specific dressings. The review concludes by addressing the challenges and future perspectives in bioactive wound dressing development. It includes regulatory considerations, clinical efficacy, patient care protocol integration and wound healing progress monitoring. Furthermore, the review considers emerging trends such as smart materials, sensors and personalised medicine approaches, offering insights into the future direction of bioactive wound dressing research.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"e13233"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142628871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patient and wound factors associated with WOUND-Q scales measuring health-related quality of life: An international cross-sectional study. 与测量健康相关生活质量的wound - q量表相关的患者和伤口因素：一项国际横断面研究

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.13245

Nina Vestergaard Simonsen, Sören Möller, Charlene Rae, Anne F Klassen, Lotte Poulsen, Andrea Louise Pusic, Jens Ahm Sørensen

{"title":"Patient and wound factors associated with WOUND-Q scales measuring health-related quality of life: An international cross-sectional study.","authors":"Nina Vestergaard Simonsen, Sören Möller, Charlene Rae, Anne F Klassen, Lotte Poulsen, Andrea Louise Pusic, Jens Ahm Sørensen","doi":"10.1111/wrr.13245","DOIUrl":"10.1111/wrr.13245","url":null,"abstract":"The WOUND-Q is a patient-reported outcome measure for individuals with any type of chronic wound. This study aimed to identify patient and wound factors associated with the four WOUND-Q health-related quality of life (HRQL) scales: Life impact, Psychological, Sleep, and Social. Adults with a chronic wound were recruited internationally through clinical settings between August 2018 and May 2020, and through an online platform (i.e. Prolific) in September 2022. Multivariable linear regression analyses were conducted to identify factors significantly associated with the WOUND-Q scales. The assessments obtained were 1273, 1275, 706, and 1256 for the Life Impact, Psychological, Sleep, and Social scales, respectively. The mean age of participants was 55 (SD = 18) years; most (66%) had a single wound, and most (56%) wounds had lasted more than 6 months. The most common causes were trauma, surgery, and diabetic foot ulcer. Wound characteristics associated with worse scores on at least one of the scales were drainage, vacuum treatment, aetiologies (i.e. diabetic foot ulcer, trauma, other, multiple), duration (i.e. 10-11 months), having four or more wounds, smell, and sleep interference, while wound location different from the face or neck was associated with better scores (p < 0.05). Patient factors associated with worse scores included having diabetes or a comorbidity, whereas increasing age or male gender were associated with better scores (p < 0.05). Sleep disturbances had the largest negative influence on HRQL scores. This study identified factors affecting HRQL in individuals with chronic wounds. Understanding these associations can inform better management and treatment strategies to improve HRQL for these patients.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e13245"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11704996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-antibiotic approaches to mitigating wound infections: Potential for SSRIs and adrenergic antagonists as emerging therapeutics. 减轻伤口感染的非抗生素方法：SSRIs和肾上腺素能拮抗剂作为新兴治疗方法的潜力。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.13240

Mirabel E Dafinone, Rawlings E Lyle, Conan Lee, Alisha Mehta, Sara E Dahle, R Rivkah Isseroff

{"title":"Non-antibiotic approaches to mitigating wound infections: Potential for SSRIs and adrenergic antagonists as emerging therapeutics.","authors":"Mirabel E Dafinone, Rawlings E Lyle, Conan Lee, Alisha Mehta, Sara E Dahle, R Rivkah Isseroff","doi":"10.1111/wrr.13240","DOIUrl":"10.1111/wrr.13240","url":null,"abstract":"Bacterial biofilms represent a formidable challenge in the treatment of chronic wounds, largely because of their resistance to conventional antibiotics. The emergence of multidrug-resistant (MDR) bacterial strains exacerbates this issue, necessitating a shift towards exploring alternative therapeutic approaches. In response to this urgent need, there has been a surge in research efforts aimed at identifying effective non-antibiotic treatments. Recently noted among the non-antibiotic options are selective serotonin reuptake inhibitors (SSRIs) and beta-adrenergic (β-AR) antagonists. Both have demonstrated antimicrobial activities and wound-healing properties, which makes them particularly promising potential therapeutics for chronic wounds. This review seeks to comprehensively evaluate the landscape of non-antibiotic strategies for managing wound infections. By analysing the latest research findings and clinical developments, it aims to shed light on emerging therapeutic alternatives. Additionally, the review delves into the potential of repurposing systemic therapeutics for topical application, offering insights into the feasibility and challenges associated with current approaches. We also address the necessity of translating promising preclinical results into tangible clinical benefits.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e13240"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A randomised, double-blind, placebo-controlled study to determine the analgesic efficacy, safety and tolerability of VPX638 administered topically to painful wounds. 一项随机、双盲、安慰剂对照研究，旨在确定VPX638局部用于疼痛伤口的镇痛疗效、安全性和耐受性。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.70008

Jonathan Golledge, Sergio Parra, Pat M Aldons, Nicoletta Frescos, Rebecca K Iseli, Toni M Pardey, Casper F Pretorius, Omar R Shum, Paul A Yates, Cécile B Bascoul, Dannette K Doolittle, Ajay A Rege, Vaidehi J Thanawala, Heather Giles, Michael C Woodward

{"title":"A randomised, double-blind, placebo-controlled study to determine the analgesic efficacy, safety and tolerability of VPX638 administered topically to painful wounds.","authors":"Jonathan Golledge, Sergio Parra, Pat M Aldons, Nicoletta Frescos, Rebecca K Iseli, Toni M Pardey, Casper F Pretorius, Omar R Shum, Paul A Yates, Cécile B Bascoul, Dannette K Doolittle, Ajay A Rege, Vaidehi J Thanawala, Heather Giles, Michael C Woodward","doi":"10.1111/wrr.70008","DOIUrl":"10.1111/wrr.70008","url":null,"abstract":"New analgesics are needed for painful wounds. Multiple reports suggest that topical sevoflurane may have analgesic effects. This placebo-controlled randomised trial evaluated the analgesic efficacy and safety of VPX638 (topical sevoflurane). Seventy-eight participants with painful wounds, were enrolled at eight Australian centres and randomly allocated to receive 2 × 5 mL of VPX638 (N = 39) or placebo (N = 40) during one wound dressing change. Numerical pain rating scores and use of opioids were recorded for 24 h. The primary endpoint was pain during wound cleaning, secondary endpoints evaluated pain for 24 h after drug application and opioids use. There was no significant difference in mean pain scores during wound cleaning between VPX638 and placebo (0.854; p = 0.23). The mean differences in summed pain intensity difference from baseline suggested VPX638 provided greater analgesia compared to placebo over 8 h (p < 0.02), 12 h (p < 0.01) and 24 h (p < 0.05) and significantly longer duration of analgesia, 24.3 h for VPX638 versus 7.1 h for placebo (p < 0.01). In the 24 h after drug administration, participants receiving VPX638 had a 50% decrease in opioid use over 24 h compared with placebo. VPX638 appeared safe and well-tolerated. In conclusion, this small placebo-controlled randomised trial suggested that VPX638 provides analgesia and is opioid-sparing for up to 24 h after wound cleaning. It supports the need for further evaluation of the benefit of VPX638 as a topical analgesic for painful wounds.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e70008"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective analysis of thermographic imaging in early detection of pressure injuries. 热成像在压力性损伤早期检测中的回顾性分析。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.70003

Olivia M Burke, Robert S Kirsner, Scott A Elman

{"title":"Retrospective analysis of thermographic imaging in early detection of pressure injuries.","authors":"Olivia M Burke, Robert S Kirsner, Scott A Elman","doi":"10.1111/wrr.70003","DOIUrl":"10.1111/wrr.70003","url":null,"abstract":"Pressure injuries in critically ill patients present a significant healthcare burden. Traditional methods, such as the Braden score, assess the risk of developing pressure injuries by evaluating factors like sensory perception, moisture and mobility. In contrast, thermographic imaging, which measures variations in skin temperature, offers a promising tool for not only assessing risk but also enabling earlier identification of pressure injuries. This study assessed thermographic imaging's ability to detect existing and evolving pressure injuries in surgical intensive care unit (SICU) patients and compared its accuracy with the Braden score. Among 465 patients, 76 underwent thermographic evaluations of the sacrum and/or heel. Of 25 patients with pressure injuries at admission, 23 had abnormal thermographic scores. Fifteen patient developed pressure injuries during SICU admission. Logistic regression showed that abnormal thermographic scores significantly increased the likelihood of detecting both existing and new injuries, while the Braden score was not a significant predictor. Thermographic imaging appears to be a superior predictor of pressure injuries, offering earlier detection and potentially improving patient outcomes while reducing healthcare costs.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e70003"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteria in hypertrophic scars promote scar formation through HSBP1-mediated autophagy. 增生性疤痕中的细菌通过hsbp1介导的自噬促进疤痕形成。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.13253

Bo Yuan, Jiarong Yu, Jiaoyun Dong, Zhigang Mao, Xiqiao Wang

{"title":"Bacteria in hypertrophic scars promote scar formation through HSBP1-mediated autophagy.","authors":"Bo Yuan, Jiarong Yu, Jiaoyun Dong, Zhigang Mao, Xiqiao Wang","doi":"10.1111/wrr.13253","DOIUrl":"10.1111/wrr.13253","url":null,"abstract":"Bacterial colonisation in hypertrophic scars (HSs) has been reported, yet the precise mechanism of their contribution to scar formation remains elusive. To address this, we examined HS and normal skin (NS) tissues through Gram staining and immunofluorescence. We co-cultured fibroblasts with heat-inactivated Staphylococcus aureus (S. aureus) and evaluated their levels of apoptosis and proliferation by flow cytometry and Cell Counting Kit-8 assay, respectively. Additionally, we performed proteomic analysis and western blotting to identify upregulated proteins. To assess autophagy levels, we examined light chain 3 (LC3) expression through western blotting and immunofluorescence, and transmission electron microscopy (TEM) was performed to detect autophagy-associated vesicles. Our results demonstrated a notable increase in bacterial load, primarily S. aureus, in HS tissues. Furthermore, S. aureus promoted fibroblast proliferation and enhanced the expression of profibrotic markers such as transforming growth factor β1 (TGF-β1), vascular endothelial growth factor (VEGF), collagen I, collagen III and α smooth muscle actin (α-SMA). Proteomic analysis highlighted heat shock factor-binding protein 1 (HSBP1) as a key upregulated protein mediating the profibrotic effects induced by S. aureus. Knockdown of HSBP1 reversed these effects. Intriguingly, HSBP1 also upregulated LC3 and Beclin-1 expression and increased the number of autophagosomes in fibroblasts. Finally, when fibroblasts stimulated by S. aureus were treated with HSBP1 siRNA, autophagy levels decreased significantly. Collectively, our findings suggest that S. aureus, via HSBP1, stimulates fibroblast proliferation and promotes their transition into myofibroblasts, triggering autophagy and fibrosis. These results underscore the potential of HSBP1 as a therapeutic target for the management of HSs.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e13253"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Wound Reporting in Animal and Human Preclinical Studies (WRAHPS) Guidelines. 动物和人类临床前研究伤口报告指南。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.13232

Nkemcho Ojeh, Nicole M Vecin, Irena Pastar, Susan W Volk, Traci Wilgus, Sarah Griffiths, Allison N Ramey-Ward, Vickie R Driver, Luisa A DiPietro, Lisa J Gould, Marjana Tomic-Canic

{"title":"The Wound Reporting in Animal and Human Preclinical Studies (WRAHPS) Guidelines.","authors":"Nkemcho Ojeh, Nicole M Vecin, Irena Pastar, Susan W Volk, Traci Wilgus, Sarah Griffiths, Allison N Ramey-Ward, Vickie R Driver, Luisa A DiPietro, Lisa J Gould, Marjana Tomic-Canic","doi":"10.1111/wrr.13232","DOIUrl":"10.1111/wrr.13232","url":null,"abstract":"Preclinical studies for wound healing disorders are an essential step in translating discoveries into therapies. Also, they are an integral component of initial safety screening and gaining mechanistic insights using an in vivo approach. Given the complexity of the wound healing process, existing guidelines for animal testing do not capture key information due to the inevitable variability in experimental design. Variations in study interpretation are increased by complexities associated with wound aetiology, wounding procedure, multiple treatment conditions, wound assessment, and analysis, as well as lack of acknowledgement of limitation of the model used. Yet, no standards exist to guide reporting crucial experimental information required to interpret results in translational studies of wound healing. Consistency in reporting allows transparency, comparative, and meta-analysis studies and avoids repetition and redundancy. Therefore, there is a critical and unmet need to standardise reporting for preclinical wound studies. To aid in reporting experimental conditions, The Wound Reporting in Animal and Human Preclinical Studies (WRAHPS) Guidelines have now been created by the authors working with the Wound Care Collaborative Community (WCCC) GAPS group to provide a checklist and reporting template for the most frequently used preclinical models in support of development for human clinical trials for wound healing disorders. It is anticipated that the WRAHPS Guidelines will standardise comprehensive methods for reporting in scientific manuscripts and the wound healing field overall. This article is not intended to address regulatory requirements but is intended to provide general guidelines on important scientific considerations for such studies.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e13232"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11621255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of incorporated additives in multifunctional dressings for chronic wound healing: An updated comprehensive review. 慢性伤口愈合的多功能敷料中添加添加剂的协同作用：一项最新的综合综述。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.13238

Alireza Sadeghi-Avalshahr, Simin Nazarnezhad, Halimeh Hassanzadeh, Mahboubeh Kazemi Noughabi, Negar Namaei-Ghasemnia, Mehdi Jalali

{"title":"Synergistic effects of incorporated additives in multifunctional dressings for chronic wound healing: An updated comprehensive review.","authors":"Alireza Sadeghi-Avalshahr, Simin Nazarnezhad, Halimeh Hassanzadeh, Mahboubeh Kazemi Noughabi, Negar Namaei-Ghasemnia, Mehdi Jalali","doi":"10.1111/wrr.13238","DOIUrl":"10.1111/wrr.13238","url":null,"abstract":"Detailed reviewing of the complicated process of wound healing reveals that it resembles an orchestrated symphony via a precise and calculated collaboration of relevant cells at the wound site. The domino-like function of various cytokines, chemokines, growth factors and small biological molecules such as antibacterial peptides all come together to successfully execute the wound healing process. Therefore, it appears that the use of a wound dressing containing only a single additive with specific properties and capabilities may not be particularly effective in treating the complex conditions that are usual in the environment of chronic wounds. The use of multifunctional dressings incorporating various additives has shown promising results in enhancing wound healing processes. This comprehensive review article explores the synergistic effects of integrated additives in such dressings, aiming to provide an updated understanding of their combined therapeutic potential. By analysing recent advancements and research findings, this review sheds light on the intricate interactions between different additives, their mechanisms of action and their cumulative impact on wound healing outcomes. Moreover, the review discusses the importance of utilising combined therapies in wound care and highlights the potential future directions and implications for research and clinical practice in the field of wound healing management.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e13238"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of TRIM72-mediated endoplasmic reticulum stress facilitates FOXM1 promotion of diabetic ulcer healing. 抑制trim72介导的内质网应激促进FOXM1促进糖尿病溃疡愈合。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2025-01-01 DOI: 10.1111/wrr.13247

Lingling Peng, Yaning Tian, Xiangkai Wu, Fengqi Liu, Mingzhu Zhou, Zixi Wu, Yumin Xia, Xiaoming Liu, Chuantao Cheng

{"title":"Suppression of TRIM72-mediated endoplasmic reticulum stress facilitates FOXM1 promotion of diabetic ulcer healing.","authors":"Lingling Peng, Yaning Tian, Xiangkai Wu, Fengqi Liu, Mingzhu Zhou, Zixi Wu, Yumin Xia, Xiaoming Liu, Chuantao Cheng","doi":"10.1111/wrr.13247","DOIUrl":"10.1111/wrr.13247","url":null,"abstract":"Foot ulcers are amongst the most prevalent complications of diabetes, known for their delayed healing process. Recent research indicates that the transcription factor forkhead box M1 (FOXM1) plays a role in promoting diabetic ulcer repair. However, the precise mechanisms underlying FOXM1 functions in this context remain unclear. This study aimed to clarify the role of tripartite motif-containing protein 72 (TRIM72)-mediated endoplasmic reticulum stress in FOXM1 promotive effects. Immunohistochemistry revealed that FOXM1 expression was significantly reduced in the lesion tissues of diabetic foot ulcer patients. In vitro experiments revealed a decrease in FOXM1 expression in cultured dermal fibroblasts under high glucose conditions. Activating FOXM1 with a plasmid accelerated the proliferation, migration, and differentiation of dermal fibroblasts and mitigated endoplasmic reticulum stress under high glucose conditions. Additionally, ChIP and luciferase reporter gene assays confirmed that FOXM1 suppressed TRIM72 expression transcriptionally by binding to its promoter. Furthermore, high glucose induced ubiquitination of adenosine 5'-monophosphate-activated protein kinase alpha (AMPKα), whilst inactivation of AMPKα signalling reversed the aforementioned effects of FOXM1 on cells. Finally, the FOXM1-overexpressing plasmid was transfected in vivo, which promoted wound healing in a murine diabetic ulcer model. In conclusion, FOXM1 reduces endoplasmic reticulum stress by inhibiting TRIM72-mediated AMPKα ubiquitination, thereby accelerating the healing of diabetic ulcers.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 1","pages":"e13247"},"PeriodicalIF":3.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0