Wound Repair and Regeneration最新文献_第5页

Histone lactylation regulates autophagy of hyperplastic scar fibroblasts by inhibiting the transcriptional activity of phosphatase and tensin homologue. 组蛋白乳酰化通过抑制磷酸酶和天丝同源物的转录活性调节增生瘢痕成纤维细胞的自噬。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-09-01 Epub Date: 2024-05-19 DOI: 10.1111/wrr.13188

Xiaosong Liu, Biao Wang

{"title":"Histone lactylation regulates autophagy of hyperplastic scar fibroblasts by inhibiting the transcriptional activity of phosphatase and tensin homologue.","authors":"Xiaosong Liu, Biao Wang","doi":"10.1111/wrr.13188","DOIUrl":"10.1111/wrr.13188","url":null,"abstract":"Hyperplastic scar (HS) is an overreaction of tissue to skin injury caused by local fibroblast proliferation and excessive collagen production. Histone posttranslational modification patterns are important epigenetic processes that control various biological activities. This study was designed to investigate the effects of histone lactylation on HS and the underlying mechanism. Western blot was used to analyse the lactylation level in HS patients and fibroblasts (HSFs). In vitro experiments, western blot, cell counting kit-8, and immunofluorescence staining were performed to detect the collagen level, cell viability, and autophagy, respectively. The relationship between snai2 (SLUG) and phosphatase and tensin homologue (PTEN) was assessed by RNA immunoprecipitation and dual-luciferase reporter assays. The results showed that the histone lactylation level was upregulated in HS tissues and HSFs. HSFs showed increased collagen production and cell viability, and decreased autophagy. Silencing of lactate dehydrogenase A (LDHA) promoted the transcription of PTEN by inhibiting SLUG, thus promoting autophagy. Knockdown of LDHA inhibited collagen deposition and cell viability, and increased autophagy in HSFs, and the results were reversed after PTEN inhibition. In summary, histone lactylation inhibited the transcription activity of PTEN by promoting SLUG, thereby suppressing autophagy and promoting collagen deposition and cell viability of HSFs, which might provide effective therapeutic strategies in HS.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"725-734"},"PeriodicalIF":3.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin fibrosis is accompanied by increased expression of secreted frizzled-related protein-2. 皮肤纤维化伴随着分泌型皱纹相关蛋白-2 的表达增加。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-09-01 Epub Date: 2024-08-19 DOI: 10.1111/wrr.13211

David M Dolivo, Adrian E Rodrigues, Lauren S Sun, Thomas A Mustoe, Seok Jong Hong, Robert D Galiano

引用次数: 0

The effect of anatomic location on porcine models of burn injury and wound healing. 解剖位置对猪烧伤和伤口愈合模型的影响。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI: 10.1111/wrr.13190

Aiping Liu, J Z Alex Cheong, Sameeha Hassan, Matthew B Wielgat, Jennifer J Meudt, Elizabeth Catherine Townsend, Dhanansayan Shanmuganayagam, Lindsay R Kalan, Angela Gibson

{"title":"The effect of anatomic location on porcine models of burn injury and wound healing.","authors":"Aiping Liu, J Z Alex Cheong, Sameeha Hassan, Matthew B Wielgat, Jennifer J Meudt, Elizabeth Catherine Townsend, Dhanansayan Shanmuganayagam, Lindsay R Kalan, Angela Gibson","doi":"10.1111/wrr.13190","DOIUrl":"10.1111/wrr.13190","url":null,"abstract":"Porcine models are frequently used for burn healing studies; however, factors including anatomic location and lack of standardised wound methods can impact the interpretation of wound data. The objectives of this study are to examine the influence of anatomical locations on the uniformity of burn creation and healing in porcine burn models. To optimise burn parameters on dorsal and ventral surfaces, ex vivo and in situ euthanized animals were first used to examine the location-dependence of the burn depth and contact time relationship. The location-dependent healing in vivo was then examined using burn and excisional wounds at dorsal, ventral, caudal and cranial locations. Lactate dehydrogenase (LDH) and H&E were used to assess burn depth and wound re-epithelialization. We found that burn depth on the ventral skin was significantly deeper than that of the dorsal skin at identical thermal conditions. Compared with burns created ex vivo, burns created in situ immediately post-mortem were significantly deeper in the ventral location. In live animals, 2 out of 12 burn wounds were fully re-epithelialized after 14 days in contrast to complete re-epithelialization of all excisional wounds. Among the burn wounds, those at the cranial-dorsal site exhibited faster healing than at the caudal-dorsal site. This study showed that anatomical location is an important consideration for the consistency of burn depth creation and healing. These data support symmetric localization of treatment and control for comparative assessment of burn healing in porcine models to prevent misinterpretation of results and increase the translatability of findings to humans.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"675-685"},"PeriodicalIF":3.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141076947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of systematic reviews of clinical studies of platelet-rich plasma for venous ulcers. 富血小板血浆治疗静脉溃疡临床研究系统综述。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-09-01 Epub Date: 2024-06-24 DOI: 10.1111/wrr.13201

Tianbo Shi, Shouci Hu, Jing Hui, Yue Ji, Yalan Zhang

引用次数: 0

Self-improving dystrophic epidermolysis bullosa with a novel heterozygous missense variant in the COL7A1 gene in a Taiwanese family. 台湾一个家族中COL7A1基因的新型杂合子错义变异导致的自发性萎缩性表皮松解症。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-07-01 Epub Date: 2024-02-28 DOI: 10.1111/wrr.13159

Yi-Chia Tsai, Wei-Ting Tu, Chun-Lin Su, Yu-Wen Cheng, Pei-Ling Chi, Chao-Kai Hsu, Yang-Yi Chen

引用次数: 0

MRSA infection, re-infection and clinical outcomes in diabetic foot infections. 糖尿病足感染中的 MRSA 感染、再感染和临床结果。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-07-01 Epub Date: 2024-02-28 DOI: 10.1111/wrr.13164

Mehmet A Suludere, Orhan K Öz, Lee C Rogers, Dane K Wukich, Matthew Malone, Lawrence A Lavery

{"title":"MRSA infection, re-infection and clinical outcomes in diabetic foot infections.","authors":"Mehmet A Suludere, Orhan K Öz, Lee C Rogers, Dane K Wukich, Matthew Malone, Lawrence A Lavery","doi":"10.1111/wrr.13164","DOIUrl":"10.1111/wrr.13164","url":null,"abstract":"The aim was to investigate methicillin-resistant Staphylococcus aureus (MRSA) incidence, conversion and outcomes in diabetic foot infections (DFIs). This is a pooled patient-level analysis of combined data sets from two randomised clinical trials including 219 patients admitted to the hospital with moderate or severe DFIs. Intraoperative bone and tissue cultures identified bacterial pathogens. We identified pathogens at index infections and subsequent re-infections. We identified MRSA conversion (MSSA to MRSA) in re-infections. MRSA incidence in index infections was 10.5%, with no difference between soft tissue infections (STIs) and osteomyelitis (OM). MRSA conversion occurred in 7.7% of the re-infections in patients who initially had MSSA in their cultures. Patients with re-infection were 2.2 times more likely to have MRSA compared to the first infection (10.5% vs. 25.8%, relative risk [RR] = 2.2, p = 0.001). Patients with MRSA had longer antibiotic treatment during the 1-year follow-up, compared to other pathogens (other 49.8 ± 34.7 days, MRSA 65.3 ± 41.5 days, p = 0.04). Furthermore, there were no differences in healing, time to heal, length of stay, re-infection, amputation, re-ulceration, re-admission, surgery after discharge and amputation after discharge compared to other pathogens. The incidence of MRSA at the index was 10.5% with no difference in STI and OM. MRSA incidence was 25.8% in re-infections. The RR of having MRSA was 2.2 times higher in re-infections. Patients with MRSA used more antibiotics during the 1-year follow-up. Furthermore, there were no differences in clinical outcomes compared to other bacterial pathogens.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"377-383"},"PeriodicalIF":3.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139991359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The infected diabetic foot: Analysis of diabetic and non-diabetic foot infections. 糖尿病足感染：糖尿病足和非糖尿病足感染分析。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-07-01 Epub Date: 2024-03-01 DOI: 10.1111/wrr.13162

Lawrence A Lavery, Mehmet A Suludere, Easton Ryan, Peter A Crisologo, Arthur Tarricone, Matthew Malone, Orhan K Oz

{"title":"The infected diabetic foot: Analysis of diabetic and non-diabetic foot infections.","authors":"Lawrence A Lavery, Mehmet A Suludere, Easton Ryan, Peter A Crisologo, Arthur Tarricone, Matthew Malone, Orhan K Oz","doi":"10.1111/wrr.13162","DOIUrl":"10.1111/wrr.13162","url":null,"abstract":"The aim of this study was to compare outcomes of moderate and severe foot infections in people with and without diabetes mellitus (DM). We retrospectively evaluated 382 patients (77% with DM and 23% non-DM). We collected demographic data, co-morbidities and one-year outcomes including healing, surgical interventions, number of surgeries, length of stay, re-infection and re-hospitalisation. DM patients required more surgeries (2.3 ± 2.2 vs. 1.7 ± 1.3, p = 0.01), but did not have a longer hospital length of stay during the index hospitalisation (DM 10.9 days ±9.2 vs. non-DM = 8.8 days ±5.8, p = 0.43). After the index hospitalisation, DM patients had increased rates of re-hospitalisation for any reason (63.3% vs. 35.2%, CI 1.9-5.2, OR 3.2, p < 0.01), re-infection at the index wound infection site (48% vs. 30.7%, CI 1.3-3.5, OR 2.1, p < 0.01), re-hospitalisation for a foot pathology (47.3% vs. 29.5%, CI 1.3-3.6, OR 2.1, p < 0.01), and longer times to ulcer healing (151.8 days ±108.8 vs. 108.8 ± 90.6 days, p = 0.04). Patients with DM admitted to hospital with foot infections have worse clinical outcomes during the index hospitalisation and are more likely to have re-infection and re-admission to hospital in the next year.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"360-365"},"PeriodicalIF":3.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139997570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of VEGF stimulation in diabetic foot ulcers: A systematic review. 刺激血管内皮生长因子对糖尿病足溃疡的影响：系统综述。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-07-01 Epub Date: 2024-03-17 DOI: 10.1111/wrr.13171

Frederik S Bang, Veronica Leeberg, Ming Ding, Chris H Dreyer

{"title":"The effect of VEGF stimulation in diabetic foot ulcers: A systematic review.","authors":"Frederik S Bang, Veronica Leeberg, Ming Ding, Chris H Dreyer","doi":"10.1111/wrr.13171","DOIUrl":"10.1111/wrr.13171","url":null,"abstract":"Diabetic foot ulcers are a common and severe complication of diabetes mellitus, and a risk factor for amputation. Because of the vessel insufficiency in diabetic foot ulcers (DFU), vascular endothelial growth factor (VEGF) that simulates angiogenesis is of interest to promote wound healing. This systematic review evaluates the last 16 years of in-vivo studies with VEGF stimulation as a treatment for DFU, developed based on the last published systematic article. A total of 961 articles were identified through databases in two phases. 947 articles were excluded by exclusion criteria, and four articles met our inclusion criteria and were included. The effects of VEGF on wound healing were analysed in all four studies. In three studies, the VEGF-treated wounds showed statistically faster healing than those not treated with VEGF. In one study, the VEGF-treated wounds revealed a positive trend toward faster healing. Furthermore, all four studies were in favor of using VEGF, but concluded that further research is needed. These studies showed a positive trend towards faster healing and was safe when using VEGF topically on humans. Furthermore, viral particles of VEGF seem to have a systematic effect when a dose exceeding 5.0 × 109 vp pr wound. Future research in using VEGF on DFU should focus on VEGF's relevant dosage, release rate, and specific mechanism. This review inspires further research, and a consistent study design is prerequisite such that results are more homogenic and comparable. Much effort is needed to translate the results into our clinical practice.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"384-392"},"PeriodicalIF":3.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outcomes of open reduction and internal fixation of calcaneus fractures: A database study comparing patients with and without diabetes. 方骨骨折切开复位内固定术的疗效：对比糖尿病患者和非糖尿病患者的数据库研究。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-07-01 Epub Date: 2024-03-22 DOI: 10.1111/wrr.13169

Matthew J Johnson, Benjamin M Conover, Robert G Frykberg, Katherine M Raspovic, Lawrence A Lavery, Dane K Wukich

{"title":"Outcomes of open reduction and internal fixation of calcaneus fractures: A database study comparing patients with and without diabetes.","authors":"Matthew J Johnson, Benjamin M Conover, Robert G Frykberg, Katherine M Raspovic, Lawrence A Lavery, Dane K Wukich","doi":"10.1111/wrr.13169","DOIUrl":"10.1111/wrr.13169","url":null,"abstract":"Treatment of calcaneal fractures in patients with diabetes mellitus (DM) is challenging. The purpose of this study was to compare post-operative outcomes after open reduction and internal fixation (ORIF) for calcaneus fracture in patients with complicated DM, uncomplicated DM, and patients without DM. A commercially available de-identified database was queried for all calcaneus fracture diagnoses undergoing ORIF from 2010 to 2021. The patients were separated into three groups for analysis: patients without DM (10,951, 82.6%), uncomplicated DM (1,500, 11.3%) and complicated DM (802, 6.1%). At 1 year, post-operative adverse events were assessed among the three groups. The odds of adverse event(s) for each group were compared between the three groups with and without characteristic matching. In the unmatched cohorts, patients with complicated DM, when compared with patients without DM and patients with uncomplicated DM, had significantly higher rates of all adverse events with exception of DVT. Rates of CNA were significantly higher in patients with complicated DM compared with no DM (OR 107.7 (CI 24.83-467.6) p < 0.0001) and uncomplicated DM (OR 44.26 (CI 3.86-507.93) p = 0.0002). After matching, non-union, AKI, sepsis, surgical site infection, and wound disruption were higher in patients with complicated DM compared with patients without DM. There were no significant differences in the three groups with regard to reoperation, DVT, MI, pneumonia, or below the knee amputation. Patients with DM who underwent ORIF for calcaneus fracture experienced higher rates of post-operative adverse events compared with those patients without DM.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"437-444"},"PeriodicalIF":3.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140185725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image-based non-invasive assessment of suction blister wounds for clinical safety and efficacy. 基于图像的无创吸疱伤口临床安全性和有效性评估。

IF 3.8 3区医学

Wound Repair and Regeneration Pub Date : 2024-07-01 Epub Date: 2024-03-21 DOI: 10.1111/wrr.13172

Karl Wallblom, Sigrid Lundgren, Karim Saleh, Artur Schmidtchen, Manoj Puthia

{"title":"Image-based non-invasive assessment of suction blister wounds for clinical safety and efficacy.","authors":"Karl Wallblom, Sigrid Lundgren, Karim Saleh, Artur Schmidtchen, Manoj Puthia","doi":"10.1111/wrr.13172","DOIUrl":"10.1111/wrr.13172","url":null,"abstract":"Recognising the need for objective imaging-based technologies to assess wound healing in clinical studies, the suction blister wound model offers an easily accessible wound model that creates reproducible epidermal wounds that heal without scarring. This study provides a comprehensive methodology for implementing and evaluating photography-based imaging techniques utilising the suction blister wound model. Our method encompasses a protocol for capturing consistent, high-quality photographs and procedures for quantifying these images via a visual wound healing score and a computer-assisted colour analysis of wound exudation and wound redness. We employed this methodology on 16 suction blister wounds used as controls in a clinical phase-1 trial. Our method enabled us to discern and quantify subtle differences between individual wounds concerning healing progress, erythema and wound exudation. The wound healing score exhibited a high inter-rater agreement. There was a robust correlation between the spectrophotometer-measured erythema index and photography-based wound redness, as well as between dressing protein content and photography-based dressing yellowness. In conclusion, this study equips researchers conducting clinical wound studies with reproducible methods that may support future wound research and aid in the development of new treatments.","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":" ","pages":"343-359"},"PeriodicalIF":3.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140176742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0