Acta Biomaterialia最新文献

{"title":"Optical Coherence Elastography Measures Mechanical Tension in the Lens and Capsule","authors":"Xu Feng ,&nbsp;Guo-Yang Li ,&nbsp;Yuxuan Jiang ,&nbsp;Owen Shortt-Nguyen ,&nbsp;Seok-Hyun Yun","doi":"10.1016/j.actbio.2025.05.009","DOIUrl":"10.1016/j.actbio.2025.05.009","url":null,"abstract":"<div><div>Lens tension is essential for accommodative vision but remains difficult to measure with precision. Here, we present an optical coherence elastography (OCE) technique that quantifies both tension and elastic modulus in the lens capsule and underlying tissue. This method derives mechanical parameters from surface wave dispersion across a critical frequency range of 1-30 kHz. Using isolated lenses from six-month-old pigs, we measured intrinsic anterior capsular tensions of 0-20 kPa and posterior capsular tensions of 40-50 kPa, induced by intra-lenticular pressure at the cortical surface. The mean shear moduli of anterior and posterior capsules were 630 kPa and 400 kPa, respectively, nearly 100-fold greater than that of the cortical tissues, where tensions were below 1 kPa. Biaxial zonular stretching (∼4% strain) increased anterior capsular tension by 67 kPa, with a low uncertainty of only 2 kPa. This optical method holds significant promise for diagnosing and managing accommodative dysfunctions through lens mechanics assessment in clinical settings.</div></div><div><h3>Statement of Significance</h3><div>Optical coherence elastography (OCE) is a rapidly advancing imaging modality, but its applications have been limited to stiffness measurements. This work represents a significant innovation by extending OCE capabilities to include force and stress quantification, broadening its potential applications in biomedical and clinical contexts. The ability to measure <em>in situ</em> capsular tension in the eye lens is a major breakthrough, as capsular tension is essential for transferring zonular fiber forces to the lens tissue during accommodation—a process critical for vision. This study provides quantitative insights into the mechanical mechanisms of accommodation and holds strong promise as a clinical tool for assessing lens tissue mechanics, addressing a capability gap in current clinical practice.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"199 ","pages":"Pages 252-261"},"PeriodicalIF":9.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil-like cell membrane-coated molybdenum-based nanoclusters for reduced oxidative stress and enhanced neurological recovery after intracerebral hemorrhage","authors":"Canxin Xu ,&nbsp;Yikui Liu ,&nbsp;Yuanbo Pan ,&nbsp;Hongchi Zhang ,&nbsp;Yuhao Sun ,&nbsp;Juan Li ,&nbsp;Aiguo Wu ,&nbsp;Liuguan Bian","doi":"10.1016/j.actbio.2025.04.035","DOIUrl":"10.1016/j.actbio.2025.04.035","url":null,"abstract":"<div><div>Excessive reactive oxygen species (ROS) are detrimental to the brain that can result in neurological impairment and inhibiting neurological functionals recovery after intracerebral hemorrhage (ICH). However, there is still a lack of effective treatment for ICH, either with medicine or neurosurgery. Nanozymes with excellent superoxide dismutase and catalase properties can scavenge ROS and may provide therapeutic opportunities for ICH patients. However, the ability of nanozymes to non-invasively target cerebral hemorrhage lesions and further antioxidation effect are still unknown. Herein, neutrophile membrane-disguised molybdenum-based polyoxometalate nanozymes (POM@Mem) were developed to alleviate oxidative stress after ICH. Coating with neutrophil membrane allowed POM to target the hemorrhage sites and further inhibit ROS generation. POM@Mem can improve neuroinflammatory microenvironment and promote behavioral improvement of ICH mouse. Combining neutrophile membrane and nanozymes for targeting brain hemorrhage sites provides an effective strategy for the treatment of ICH.</div></div><div><h3>Statement of significance</h3><div>Excessive reactive oxygen species (ROS) are detrimental to the brain and can lead to neurological impairment, hindering the recovery of neurological functions after intracerebral hemorrhage (ICH). Despite this, effective treatments for ICH, whether pharmaceuticals or neurosurgery, remain scarce. In this study, we developed neutrophil membrane-disguised molybdenum-based polyoxometalate nanozymes (POM@Mem) as a novel approach to alleviate oxidative stress following ICH. The neutrophil membrane coating enabled the POM nanozymes to specifically target hemorrhagic sites, thereby inhibiting ROS production. Additionally, POM@Mem improved the neuroinflammatory microenvironment and facilitated behavioral recovery in ICH mice. The combination of neutrophil membranes and nanozymes for targeted delivery to brain hemorrhage sites offers a promising strategy for the treatment of ICH.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"199 ","pages":"Pages 337-345"},"PeriodicalIF":9.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetothermal and ultrasound-activated nanoplatform for the inhalable therapy of bacterial lung infections","authors":"Shuai Zhang ,&nbsp;Yundi Wu ,&nbsp;Chaoyi Lyu ,&nbsp;Huanran Qu ,&nbsp;Xilong Wu","doi":"10.1016/j.actbio.2025.04.041","DOIUrl":"10.1016/j.actbio.2025.04.041","url":null,"abstract":"<div><div>Antibiotic resistance in <em>Klebsiella pneumoniae</em> infections presents significant challenges in treating lung inflammation. To overcome tissue penetration barriers and modulate inflammatory responses, innovative therapeutic approaches are essential. This study introduces an inhalable nanoplatform, Fe_xS_y:Gd@PVP (FGP), based on polyvinylpyrrolidone-modified gadolinium-doped nonstoichiometric iron sulfide nanostructures. The platform integrates synergistic magnetic-ultrasound activation with magnetothermal therapy (mMHT), sonodynamic therapy (SDT), and gas therapy (GT) for targeted bacterial lung infection treatment. Gadolinium incorporation enhances the magnetothermal activation, improving magnetothermal conversion efficiency and sonodynamic performance by increasing magnetic anisotropy, narrowing the semiconductor bandgap, and enriching sulfur vacancies. Delivered <em>via</em> nebulized inhalation, FGP reaches infected lung tissues noninvasively. Exposure to alternating magnetic fields (AMF) and ultrasound (US) generates localized heat and reactive oxygen species (ROS), effectively eliminating bacteria. Simultaneously, AMF and US trigger hydrogen sulfide (H₂S) release in the acidic microenvironment, reducing inflammation by inhibiting inflammatory factors such as TNF-α and IL-1β through suppression of STAT3 and ERK phosphorylation. This magnetic-ultrasound co-activated inhalable nanoplatform offers a powerful multimodal therapeutic strategy for overcoming clinical challenges associated with bacterial lung infections.</div></div><div><h3>Statement of significance</h3><div>This study introduces an inhalable nanoplatform that effectively treats multidrug-resistant <em>Klebsiella pneumoniae</em> lung infections. By integrating magnetothermal, sonodynamic, and gas therapies, this system eradicates bacteria and reduces inflammation. It uses gadolinium-doped iron sulfide nanostructures to enhance heat, reactive oxygen species, and hydrogen sulfide production, targeting deep lung infections precisely. Unlike traditional antibiotics, this noninvasive approach has minimal side effects and addresses both bacterial clearance and inflammation. This innovative strategy offers a promising solution for antibiotic-resistant infections and could revolutionize respiratory disease management.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"199 ","pages":"Pages 428-442"},"PeriodicalIF":9.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondria-targeted manganese-based mesoporous silica nanoplatforms trigger cGAS-STING activation and sensitize anti PD-L1 therapy in triple-negative breast cancer","authors":"Nan Zhong ,&nbsp;Ziyue Zu ,&nbsp;Yishi Lu ,&nbsp;Xuan Sha ,&nbsp;Yang Li ,&nbsp;Yang Liu ,&nbsp;Shangyu Lu ,&nbsp;Xi Luo ,&nbsp;Yan Zhou ,&nbsp;Jun Tao ,&nbsp;Feiyun Wu ,&nbsp;Zhaogang Teng ,&nbsp;Yuxia Tang ,&nbsp;Shouju Wang","doi":"10.1016/j.actbio.2025.04.040","DOIUrl":"10.1016/j.actbio.2025.04.040","url":null,"abstract":"<div><div>Activation of the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway could effectively initiate antitumor immunity in triple-negative breast cancer. However, current nuclear DNA-mediated activation of STING pathway remains constrained by the tight protection of nuclear membrane and histones, highlighting the need for new strategies to enhance its efficacy. Mitochondrial DNA (mtDNA), in contrast, is more vulnerable to damage. Herein, our nanoplatforms exploited the high glutathione (GSH) environment characteristic of tumors to release abundant Mn^b+, which induced mitochondrial dysfunction and the release of endogenous mtDNA. The released mtDNA, in conjunction with Mn^b+ itself functioning as a strong cGAS agonist, effectively activated cGAS-STING pathway. Consequently, the cGAS-STING-dependent secretion of type I interferon successively enhanced the maturation of dendritic cells and cross-priming of CD8⁺ T cells. In a poorly immunogenic 4T1 tumor model, TPP-MMONs efficiently primed systemic antitumor immunity and significantly enhanced the therapeutic efficacy of αPD-L1 therapy, suppressing tumor growth in both localized and metastatic tumor models. These findings provided an innovative and straightforward strategy to enhance TNBC immunogenicity by targeting mitochondrial damage to induce mtDNA-mediated cGAS-STING activation, thereby sensitizing tumors to immune checkpoint inhibitor therapy.</div></div><div><h3>Statement of significance</h3><div>The cGAS-STING pathway is a promising target for overcoming immunoresistance in TNBC. However, current nuclear DNA-based activation strategies are limited by the tight protection of nuclear membrane and histones. Herein, we reported novel manganese-rich, mitochondria-targeting nanoplatforms (TPP-MMONs), which can release abundant Mn²⁺ and significantly induce mitochondrial dysfunction, leading to the release of mtDNA. As a result, the nanoplatforms can effectively stimulate the cGAS-STING pathway, thereby enhancing immune responses and improving the therapeutic efficacy of αPD-L1 therapy, offering new insights into TNBC treatments.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"199 ","pages":"Pages 374-386"},"PeriodicalIF":9.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catechol crosslinked bioprosthetic valves derived from caffeic acid and dopamine-conjugated porcine pericardia exhibit enhanced antithrombotic, immunomodulatory and anticalcification performance","authors":"Lepeng Chen ,&nbsp;Bangquan Wei ,&nbsp;Xueyu Huang ,&nbsp;Li Yang ,&nbsp;Rifang Luo ,&nbsp;Cheng Zheng ,&nbsp;Yunbing Wang","doi":"10.1016/j.actbio.2025.04.054","DOIUrl":"10.1016/j.actbio.2025.04.054","url":null,"abstract":"<div><div>The global aging population has led to an increasing prevalence of valvular heart disease (VHD), and the clinical application of bioprosthetic heart valves (BHVs) are growing with the advancement of transcatheter heart valve replacement surgery. However, BHVs, as xenogeneic pericardial tissue crosslinked with glutaraldehyde, have been affected by suboptimal cytocompatibility, thrombosis, immune response, and calcification, leading to premature degeneration and failure. Herein, a catechol-crosslinking strategy for BHVs was developed by conjugating porcine pericardia (PP) with catechols and subsequently coupling the grafted catechols to achieve the crosslinking and stabilization of BHVs. Caffeic acid and dopamine were exploited to conjugate the bioactive catechols on PP through amide condensation, and the catechols were further coupled under oxidation to impart the PP with enhanced stability and cytocompatibility as well as comparable mechanical properties to those of glutaraldehyde crosslinked PP (GLUT-PP). With the enrichment of catechols, the crosslinked PP not only demonstrated improved hydrophilicity to resist the blood components adhesion and thrombosis, but also enhanced the performance of endothelialization and antioxidation. Furthermore, the introduced catechols exhibits favorable anti-inflammatory properties, which significantly ameliorated the foreign body response and regulated the local immune responses of crosslinked PP. In conclusion, the catechol crosslinked PP is expected to be explored as a potential substitute for GLUT-PP to extend the lifespan of BHVs.</div></div><div><h3>Statement of significance</h3><div>Bioprosthetic heart valves (BHVs) are mainly prepared from glutaraldehyde crosslinked porcine or bovine pericardia (GLUT-PP). Currently, BHVs are affected by cytotoxicity, thrombosis, calcification, and immunoinflammatory responses, which would accelerate degeneration and failure of BHVs. In this study, we developed a catechol crosslinking strategy for BHVs and engineered caffeic acid and dopamine-conjugated porcine pericardia (PP). In summary, catechol crosslinked porcine pericardia demonstrated enhanced collagen stability, antithrombosis, endothelialization, anticalcification and immunomodulation which reduced the risk of structural degeneration, suggesting that the catechol crosslinked porcine pericardia could serve as a potential alternative to GLUT-PP.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"199 ","pages":"Pages 90-107"},"PeriodicalIF":9.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144037173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A smart capsule with a bacteria- and pH-triggered enteric polymer coating for targeted colonic microbiome sampling","authors":"Devendra Sarnaik ,&nbsp;Akshay Krishnakumar ,&nbsp;Sina Nejati ,&nbsp;Caitlyn R. Sullivan ,&nbsp;Tzu-Wen L. Cross ,&nbsp;Wayne W. Campbell ,&nbsp;Jay S. Johnson ,&nbsp;Rahim Rahimi","doi":"10.1016/j.actbio.2025.04.025","DOIUrl":"10.1016/j.actbio.2025.04.025","url":null,"abstract":"&lt;div&gt;&lt;div&gt;The gut microbiome is recognized as a critical factor in advancing precision nutrition and medicine for health and in developing dietary recommendations and targeted therapies for gastrointestinal (GI) health and diseases. However, conventional sampling methods, such as fecal analysis and colonoscopy, often fail to capture microbial information from specific regions of the GI tract or require invasive procedures, thereby limiting accuracy and clinical utility. As a non-invasive alternative, passive sampling capsules have been developed for site-specific microbiome analysis by employing pH-sensitive enteric coatings that delay sampling until the capsule reaches the targeted intestinal region. Although this approach has been successful in the small intestine, colonic sampling remains challenging due to the high interpersonal variability in intestinal pH, which makes it difficult to rely solely on a pH-triggering mechanism. To overcome this challenge, a dual bacterially and pH triggered polymeric enteric coating was created by blending lactulose and N,N-dimethylaminoethyl methacrylate, enabling complete dissolution within the colonic region. Through systematic characterization of multiple polymer blend compositions using Fourier Transform Infrared Spectroscopy, Thermogravimetric Analysis, and Differential Scanning Calorimetry, an optimized design was identified that provides both suitable physical integrity and rapid (∼2 h) degradation in the presence of colonic bacteria, across a pH range of 5 to 8. The optimized blend was subsequently applied as a double-layer enteric coating on a sampling capsule, enabling the dissolution of the outer layer in the small intestine and complete dissolution of the inner layer in the colon. &lt;em&gt;In-vitro&lt;/em&gt; and &lt;em&gt;in-vivo&lt;/em&gt; pig model studies were conducted to validate the capsule's sampling performance and to ensure the preservation of the microbial environment. Furthermore, 16S rRNA sequencing revealed a taxonomic similarity between samples collected by the capsule and the colonic microbiome (residing between the ileum and fecal matter). Overall, this technology provides an effective approach to targeted microbial sampling and may pave the way for more comprehensive colonic microbiome analyses and improved diagnostic capabilities for GI diseases.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Statement of significance&lt;/h3&gt;&lt;div&gt;Precise monitoring of the gut microbiome is vital for understanding health and disease, yet current sampling techniques often lack precision or require invasive procedures. Our work introduces a novel, non-invasive capsule that targets the colon using a dual-trigger polymer system activated by both pH and colonic bacteria. This design enables localized sampling of gut microbiota, overcoming the limitations of fecal analysis, endoscopy, and earlier pH-triggered capsule designs. By capturing microbial communities directly from the colon, our technology provides deeper insights into colonic health and condit","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"199 ","pages":"Pages 412-427"},"PeriodicalIF":9.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolution in Organ Preservation: Technological Exploration","authors":"Xin-Yuan Kang ,&nbsp;Jia-Yi Cheng ,&nbsp;Wan-Yi Ge,&nbsp;Yi-Ming Tong,&nbsp;Da-Chuan Yin","doi":"10.1016/j.actbio.2025.05.008","DOIUrl":"10.1016/j.actbio.2025.05.008","url":null,"abstract":"<div><div>Organ preservation plays a critical role in addressing transplantation challenges, including donor shortages and ischemia-reperfusion injury (IRI). Continuous advancements in preservation technologies are essential to meet the increasing demand for transplantable organs. This review provides a comprehensive analysis of organ preservation techniques, spanning from hypothermic storage to advanced methods such as supercooling, vitrification, and partial freezing. Historical milestones, including the development of the EuroCollins, University of Wisconsin (UW), ET-Kyoto, and Celsior solutions, are discussed alongside innovations in machine perfusion and cryopreservation technologies. Particular emphasis is placed on the underlying mechanisms of these techniques, such as metabolic rate suppression, prevention of ice crystal formation, and the application of cryoprotectants, all aimed at extending preservation duration and improving organ quality. Emerging trends, such as the integration of nanotechnology and artificial organ cultivation, are highlighted as promising directions to enhance preservation efficiency. By exploring current advancements and future trends, this review underscores the importance of technological innovation in addressing the global organ shortage crisis and improving transplantation outcomes.</div></div><div><h3>Statement of Significance</h3><div>This review offers a comprehensive analysis of the advancements in organ preservation technologies, a critical area in addressing the global organ shortage crisis. By detailing the evolution from early perfusion techniques to cutting-edge innovations like supercooling, vitrification, and nanotechnology, the work underscores the importance of extending organ viability and improving transplant outcomes. Importantly, it bridges historical milestones with emerging trends, showcasing how integration of novel materials and methodologies can revolutionize organ transplantation. This work not only enriches the scientific understanding of organ preservation but also opens pathways for interdisciplinary innovations, setting the stage for the development of sustainable and efficient organ banks. By aligning technological advancements with clinical challenges, it provides actionable insights that could reshape transplantation medicine.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"199 ","pages":"Pages 50-73"},"PeriodicalIF":9.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging the microstructure of the arterial wall – ex vivo to in vivo potential","authors":"B. Tornifoglio ,&nbsp;C. Hughes ,&nbsp;F. Digeronimo ,&nbsp;Y. Guendouz ,&nbsp;R.D. Johnston ,&nbsp;C. Lally","doi":"10.1016/j.actbio.2025.05.022","DOIUrl":"10.1016/j.actbio.2025.05.022","url":null,"abstract":"<div><div>Microstructural imaging enables researchers to visualise changes in the arterial wall, allowing for (i) a deeper understanding of the role of specific components in arterial mechanics, (ii) the observation of cellular responses, (iii) insights into pathological alterations in tissue microstructure, and/or (iv) advancements in tissue engineering aimed at replicating healthy native tissue. In this prospective review, we present various imaging modalities spanning from ex vivo to <em>in vivo</em> applications within arterial tissue. The pros, cons, and sensitivities of these modalities are highlighted. By consolidating the latest advancements in microstructural imaging of arterial tissue, the authors aim for this paper to serve as a guide for researchers designing experiments at various stages. Furthermore, the integration of non-invasive, non-destructive imaging techniques into studies provides an additional layer of microstructural information, enhancing scientific findings, improving our understanding of disease, and potentially enabling earlier or more effective diagnostic capabilities.</div></div><div><h3>Statement of significance</h3><div>Imaging the specific microstructural components of the arterial wall provides critical insights into vascular biology, mechanics, and pathology. It enables the visualisation of key structural components and their roles in arterial function, supports the analysis of cell-matrix interactions, and reveals microarchitectural changes associated with disease progression. This level of specificity also informs the design of biomimetic materials and scaffolds in tissue engineering, facilitating the replication of native arterial properties.</div><div>By synthesising recent developments in microstructural imaging techniques, this paper serves as a reference for investigators designing experiments across a range of vascular research applications. Moreover, the incorporation of non-invasive, non-destructive imaging methods offers a means to acquire detailed microstructural data without compromising tissue integrity. This enhances the interpretability and translational potential of findings, deepens our understanding of vascular disease mechanisms, and may ultimately contribute to the development of earlier and more precise diagnostic approaches.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"199 ","pages":"Pages 18-34"},"PeriodicalIF":9.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macromolecular crowding-based biofabrication utilizing unmodified extracellular matrix bioinks","authors":"Seyma Nayir Jordan ,&nbsp;Xianmu Li ,&nbsp;Alejandro Rossello-Martinez ,&nbsp;Zixie Liang ,&nbsp;Xiangyu Gong ,&nbsp;Hugh Xiao ,&nbsp;Michael Mak","doi":"10.1016/j.actbio.2025.02.052","DOIUrl":"10.1016/j.actbio.2025.02.052","url":null,"abstract":"<div><div>The extracellular matrix (ECM) is the body's natural cell-scaffolding material, and its structure and content are often imitated for applications in tissue engineering and regenerative medicine to promote biocompatibility. One approach toward biomimicking natural ECMs is to utilize decellularized extracellular matrices (dECMs), which involve removing cellular components from native tissues to preserve natural components. Solubilizing dECMs to produce bioinks therefore holds high potential for 3D biofabrication and bioprinting of bioactive scaffolds and tissues. However, solubilized ECMs have low printability owing to their slow gelation times, which necessitates additional artificial modifications (e.g. crosslinking) to facilitate biofabrication applications. In this study, we demonstrate a method utilizing macromolecular crowding (MMC) to confer printability, via rapid gelation, to solubilized unmodified dECMs from a variety of tissue types - heart, muscle, liver, small intestine, and large intestine. We show cell spreading and contractility in cell-laden dECM gels fabricated through MMC, highlighting biocompatibility with our method. Finally, we demonstrate successful extrusion bioprinting of complex 3D structures using unmodified dECM solutions as bioinks, revealing the potential of our MMC-based fabrication method for layer-by-layer building of user-designed bioinks made from wide-ranging fully physiological tissues.</div></div><div><h3>Statement of significance</h3><div>Decellularized extracellular matrix (dECM) bioinks are among the most promising materials for simulating native organ-specific extracellular matrices. However, standard methods for gelling solubilized dECMs are slow and result in poor mechanical and structural characteristics, reducing printability. dECM solutions are typically supplemented with additional crosslinkers for the formation of robust hydrogels. The crosslinkers may be toxic to cells, and they often need UV light for activation. Here, we present a method that allows wide-ranging dECMs to be easily patternable and 3D printable in their unmodified forms. We demonstrate cell spreading and contractility in cell-laden unmodified dECM gels created demonstrating cell viability and bioactivity. We also demonstrated successful extrusion bioprinting of complex 3D structures utilizing low concentration unmodified dECM bioinks and normal healthy lung fibroblasts.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 37-48"},"PeriodicalIF":9.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Effect of cell imprinting on viability and drug susceptibility of breast cancer cells to doxorubicin” [Acta Biomaterialia, 2020, 113, 119-129]","authors":"Fatemeh Shahriyari ,&nbsp;Mohsen Janmaleki ,&nbsp;Shahriar Sharifi ,&nbsp;Milad Eyvazi Hesar ,&nbsp;Sasha Hoshian ,&nbsp;Reza Taghiabadi ,&nbsp;Ahmad Razaghian ,&nbsp;Majid Ghadiri ,&nbsp;Afshin Peirovi ,&nbsp;Morteza Mahmoudi ,&nbsp;Amir Sanati Nezhad ,&nbsp;Ali Khademhosseini","doi":"10.1016/j.actbio.2025.04.007","DOIUrl":"10.1016/j.actbio.2025.04.007","url":null,"abstract":"","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 571-572"},"PeriodicalIF":9.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}