World journal of microbiology & biotechnology最新文献

{"title":"Deciphering the potential antagonistic activity of Bacillus velezensis GNB3-7 against Astragalus mongholicus root rot.","authors":"Ruhan Xia, Pengxiang Han, Qiangbo Zhao, Wenzhi Yao, JingJing Jiang, Miaoyin Dong, Mengfei Li","doi":"10.1007/s11274-026-05290-5","DOIUrl":"10.1007/s11274-026-05290-5","url":null,"abstract":"<p><p>Astragalus mongholicus is one of the most commonly traditional Chinese herbal medicines in China and other Asian countries. However, the root rot disease caused by Fusarium oxysporum can severely reduce root yield and quality, resulting in significant large economic losses. Although biological control has attracted widespread attention due to its safety, environmental friendliness and sustainability, the biocontrol efficiency and potential mechanism of Bacillus velezensis against root rot of A. mongholicus have been rarely investigated. In the present study, a rhizosphere bacterium GNB3-7 was isolated from the rhizosphere soil of wild A. mongholicus and possessed the strong broad-spectrum antagonistic activity on multiple pathogenic fungi, which was identified as B. velezensis on the basis of whole-genome sequence analyses. Further genomic analyses revealed that the B. velezensis GNB3-7 has 12 biosynthetic gene clusters encoding antifungal secondary metabolites (e.g., surfactin, bacillibactin, bacilysin). In addition, a total of 36 volatile organic compounds (VOCs) were detected by GC-MS, among which benzothiazole, disulfide-dimethyl and 2-nonanone showed the strongest antifungal activities, with an inhibition rate of 99.41%, 96.87% and 92.69%, respectively. In pot experiments, the treatment rate and prevention efficiency of B. velezensis GNB3-7 against A. mongholicus root rot achieved 44.44% and 72.22%, respectively. Notably, the genes involved in plant induced systemic resistance (ISR, e.g., AmNPR1, AmCHI2 and AmCOI1) were significantly up-regulated in treatment and prevention plants, indicating the activation of ISR in A. mongholicus. These results indicated that B. velezensis GNB3-7 could efficiently control root rot by synergistic action involving the production of metabolites and VOCs, activation of plant ISR, which deepens our understanding on the biocontrol mechanism of B. velezensis against root rot in medicinal plant A. mongholicus.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic and phenotypic profiling of commercial probiotic Enterococcus faecium strains: insights into safety and functional attributes.","authors":"Yongqi Gan, Manman Lu, Lanyan Fan, Jun Nong, Pingping Shangguan, Bin Zhu, Peng Xie, Zan Zhang","doi":"10.1007/s11274-026-05284-3","DOIUrl":"10.1007/s11274-026-05284-3","url":null,"abstract":"<p><p>Enterococcus faecium is widely used in probiotics but can also act as an opportunistic pathogen, necessitating strain-specific safety and efficacy assessments. While previous studies have characterized individual E. faecium probiotic strains, few have provided an integrated genomic and phenotypic comparison of multiple commercial strains from the Asian market. This study conducted a comparative genomic and phenotypic analysis of five E. faecium strains isolated from commercial probiotic products alongside five reference strains. Whole-genome sequencing revealed that the probiotic-derived strains, which were identified as Enterococcus lactis, clustered into two distinct phylogenetic clades (Clade I: ST-812; Clade II: ST-76), corroborated by Average Nucleotide Identity and Multilocus Sequence Typing analyses. These clades exhibited coherent functional genomic profiles, with Clade I enriched in genes for carbohydrate metabolism and dietary fiber degradation, while Clade II showed a different emphasis, including carotenoid biosynthesis. Crucially, all probiotic strains lacked high-risk antibiotic resistance genes (vanA) and major virulence determinants (esp, hyl, gelE) found in the clinical control strain ATCC 51559, and were phenotypically susceptible to the tested β-lactams (penicillin, ampicillin), vancomycin, and tetracycline, and lacked acquired resistance genes for WHO-defined critically important antibiotics. In vitro probiotic property assays further showed significant inter-strain variability in gastric acid tolerance, bile salt resistance, and bile salt hydrolase (BSH) activity, underscoring the strain-specific nature of functional performance. Our integrated analysis indicates a strong link between genomic identity, functional potential, and safety profile in commercial E. faecium probiotics. This study provides a detailed genomic framework for strain-level safety assessment and supports the informed selection of E. faecium strains for probiotic applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity of Burkholderia sensu lato metabolites against coffee phytopathogens: evidence of chemical diversity.","authors":"Irma Indira Jiménez-Gómez, Vianey Marín-Cevada, Luis Ernesto Fuentes-Ramírez, Gloria Carrión, Ricardo Enrique Buendia-Corona, Esmeralda García-Díaz","doi":"10.1007/s11274-026-05282-5","DOIUrl":"10.1007/s11274-026-05282-5","url":null,"abstract":"<p><p>The environmental and health concerns associated with the widespread use of synthetic pesticides have intensified the search for sustainable alternatives to control phytopathogenic fungi. In this context, members of the Burkholderia sensu lato group have been recognized for their ability to produce diverse bioactive metabolites with antifungal properties. In this study, the antifungal activity of 36 rhizosphere-derived strains was evaluated against Hemileia vastatrix, Fusarium solani, Fusarium oxysporum, and Alternaria longissima. Approximately 80% of the strains inhibited the uredospore germination of H. vastatrix, while 37%, 50%, and 45% reduced radial growth of F. solani, F. oxysporum, and A. longissima, respectively. Selected strains (GB99, D335, and D416) produced heat-stable metabolites that retained activity across a wide pH range and showed partial resistance to hydrolytic enzymes. Metabolomic profiling by high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS), performed on metabolites produced by strain GB99, revealed multiple putative antifungal compounds, including burkholdine, occidiofungin and lagriamide related metabolites. Molecular docking, used as an exploratory approach, suggested potential interactions with multiple fungal protein targets. Overall, these findings highlight the chemical diversity and antifungal potential of Burkholderia sensu lato metabolites as promising candidates for sustainable biocontrol strategies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioconversion of agricultural waste to ε-poly-L-lysine by an engineered Streptomyces albulus strain.","authors":"Rui Huang, Ziling Yuan, Yujie Liu, Liwen Zhang, Guanhua Li, Ruihua Liu, Chao Yang","doi":"10.1007/s11274-026-05283-4","DOIUrl":"10.1007/s11274-026-05283-4","url":null,"abstract":"<p><p>The vast majority of agricultural wastes are burned or buried in soil currently, causing environmental pollution and resource waste worldwide. To achieve the eco-friendly bioconversion of agricultural waste, this study utilized an engineered Streptomyces albulus for solid-state fermentation (SSF) to convert rapeseed cake into ε-poly-L-lysine (ε-PL), the L-lysine homopolymer widely applied as a natural and safe food preservative. Firstly, based on genome analysis and the property characterization of agricultural waste, the bioconversion potential of S. albulus toward lignocellulosic waste was revealed, followed by preliminary experimental validation. Secondly, the high-titer engineered strain was obtained by overexpressing key genes eno1, eno2, pepc, ask and pls of ε-PL biosynthesis pathway, thereby promoting the bioconversion of rapeseed cake. Among the mutants, S. albulus PLS got the highest ε-PL titer, which was 9.3-fold that of the control strain in SSF. And the ε-PL titer was further increased to 1.6 times the original via optimizing SSF process. Finally, we characterized the growth and development status and the lignocellulose biodegradation of S. albulus PLS during the bioconversion process. Overall, this study identified a strategy for the valorization of agricultural waste, and the engineered strain offers a promising SSF microbial chassis for ε-PL production from lignocellulosic waste.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reuterin alleviates Enterohemorrhagic Escherichia coli O157:H7-induced infectious diarrhea in mice through modulation of the gut microbiota.","authors":"Yuhao Wang, Dongming Zhou, Qiong Chen, Changjun Wang, Wenhao Li, Xuewei Sun, Chengliang Tang, Zhiqiang Jiang, Xuefei Zhang, Wenjing Wang, Zhan Yang, Qian Cui","doi":"10.1007/s11274-026-05281-6","DOIUrl":"10.1007/s11274-026-05281-6","url":null,"abstract":"<p><p>Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) is a globally significant foodborne pathogen. Infection disrupts the gut microbiota and exacerbates host immune dysfunction. Reuterin, a broad-spectrum antimicrobial metabolite produced by Limosilactobacillus reuteri during glycerol fermentation, exhibits potent antibacterial activity in vitro; however, its in vivo preventive efficacy and underlying mechanisms remain incompletely understood. This study aimed to comprehensively evaluate the protective effects of reuterin prophylaxis and to investigate its potential mechanisms in mitigating EHEC O157:H7 infection through gut microbiota modulation. In a murine infection model, prophylactic administration of reuterin (60 mM) conferred pronounced protection, as evidenced by alleviation of colonic pathology, oxidative stress, and systemic inflammation, together with restoration of intestinal barrier integrity. 16S rRNA sequencing and fecal microbiota transplantation (FMT) demonstrated that reuterin alleviated EHEC O157:H7 infection through gut microbiota remodeling, which was associated with elevated abundances of Ligilactobacillus and Alloprevotella and increased short-chain fatty acids (SCFAs) production. Exogenous SCFAs supplementation was associated with attenuated colonic damage and inflammation, consistent with a potential contribution of these metabolites to the observed protection. Furthermore, our data are compatible with a model wherein SCFAs-mediated activation of the intestinal receptor GPR43 may suppress NF-κB signaling. Collectively, this study provides a foundation for the further development of reuterin as a prophylactic agent against EHEC O157:H7 infection.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and metabolomic insights into carotenoid biosynthesis in a novel deep-sea bacterium, Paracoccus niotae sp. nov., isolated from an Arabian Sea seamount.","authors":"Abirami Alagarsamy, Pankaj Verma, Harini Deivendran, Thenaruvi Sundaramurthy, Gopal Dharani, Balaji Ramakrishnan","doi":"10.1007/s11274-026-05277-2","DOIUrl":"10.1007/s11274-026-05277-2","url":null,"abstract":"<p><p>Natural microbial carotenoids are increasingly recognized as alternatives to synthetic pigments because of their broad applications in the food, pharmaceutical, nutraceutical, cosmetic, feed, and aquaculture industries. In this study, a bright orange-pigmented deep-sea bacterium, strain NIOT.M2S5SZ12ᵀ, isolated from Arabian Sea seamount sediment, was comprehensively characterized to evaluate its carotenoid biosynthetic potential and taxonomic status. Phylogenetic analysis placed the strain within the genus Paracoccus, while whole-genome sequencing generated a 3.6 Mb draft genome with a GC content of 68.50 mol%. Genome-based taxonomic analyses yielded maximum digital DNA-DNA hybridization and average nucleotide identity values of 30.9% and 86.39%, respectively, confirming that the isolate represents a novel species within the genus. Comparative pan-genome analysis revealed an open pan-genome comprising 19,613 gene clusters, including 1,288 strain-specific genes. Phenotypic, physiological, and chemotaxonomic analyses further distinguished the isolate from closely related Paracoccus type strains. Genome mining using antiSMASH and KEGG pathway reconstruction identified key carotenoid biosynthesis genes (idi, crtE, crtB, crtI, crtY, crtZ, and crtW). The strain produced 67.93 ± 3.76 mg g⁻¹ dry biomass of crude pigment, and UHPLC-APCI-Orbitrap MS/MS confirmed the production of astaxanthin, zeaxanthin, canthaxanthin, retinal, and several apo-carotenoid derivatives. Based on comprehensive phenotypic, chemotaxonomic, phylogenetic, genomic, and metabolomic evidence, strain Z12 is proposed as Paracoccus niotae sp. nov. NIOT.M2S5SZ12ᵀ under the SeqCode framework. These findings establish Paracoccus niotae as a promising marine microbial resource for sustainable carotenoid production and biotechnology.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative-genomics-guided development of an isothermal assay for rapid detection of Fusarium oxysporum f. sp. phaseoli.","authors":"Kun Qian, Yuxin Zhang, Yang Chen, Simeng Chang, Guohua Chen, Jianlong Zhao, Zhenchuan Mao, Jian Ling, Yanlin Li","doi":"10.1007/s11274-026-05288-z","DOIUrl":"10.1007/s11274-026-05288-z","url":null,"abstract":"<p><p>Accurate identification of closely related lineages within the Fusarium oxysporum species complex remains challenging because commonly used DNA barcode regions often provide insufficient resolution at the forma specialis level. In this study, comparative genomics approach was combined with enzyme-mediated duplex exponential amplification (EmDEA) to develop a rapid molecular assay for F. oxysporum f. sp. phaseoli (FOP). A FOP strain LM-2 was characterized by morphological, molecular, and pathogenicity analyses. A chromosome-level genome assembly of the strain comprising 14 pseudochromosomes was generated using short-read, long-read, and Hi-C sequencing. Comparative analysis with seven other F. oxysporum genomes identified 1.004 Mb of candidate diagnostic regions. A 9.82-kb region on chromosome 11 was selected, and a 1,032-bp fragment designated TC4-2 was experimentally validated and used for primer and probe design. The resulting one-tube EmDEA assay was completed at 42 °C within 30 min. Positive amplification was obtained from three tested FOP isolates, whereas no effective amplification was observed in 12 tested non-target fungi. The conservative analytical detection limit was 40 pg µL⁻¹ purified genomic DNA. The assay also detected FOP DNA in purified DNA extracted from diseased cowpea stems and field soil. These results demonstrate a comparative-genomics-guided workflow for developing rapid closed-tube assays for closely related fungal pathogens.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus-derived selenium nanoparticles modulate chromium(VI) toxicity through regulation of metal uptake and oxidative stress in chickpea (Cicer arietinum L.).","authors":"Soumita Roy, Abir Das, Nilakshi Chakraborty, Arup Kumar Sarkar, Ribhu Maity, Tithi Maity, Bidhan Chandra Samanta, Gourav Mondal, Pradip Bhattacharyya, Anway Ganguly, Sudipta Kumar Sil, Satish V Patil, Vishnu D Rajput, Malay Kumar Adak","doi":"10.1007/s11274-026-05271-8","DOIUrl":"10.1007/s11274-026-05271-8","url":null,"abstract":"<p><p>This study explores the bioinorganic potential of selenium nanoparticles (SeNPs) biosynthesized by Bacillus sp. (AKS_bp1, PQ824612) in mitigating hexavalent chromium [Cr(VI)] toxicity in chickpea (Cicer arietinum L.). SeNPs were produced via microbial reduction of sodium selenite and characterized by nanoscale size (~ 100-150 nm), negative surface charge (- 20 mV), and biomolecular capping. Under controlled hydroponic Cr(VI) exposure (0-100 ppm), SeNP treatment significantly reduced Cr accumulation in plant tissues, with decreases of 24% in roots and 45% in shoots, indicating restricted translocation and improved metal homeostasis. This suggests a Se-mediated modulation of Cr mobility within the plant system. At the physiological and biochemical levels, SeNPs alleviated Cr-induced oxidative stress, as evidenced by reductions in superoxide (65%) and hydrogen peroxide (52%) levels, along with enhanced antioxidant defense and photosystem II efficiency (a 21% increase in photochemical performance). Improved chlorophyll fluorescence and stomatal regulation further indicated restoration of cellular functionality under metal stress. Importantly, SeNPs exhibited intrinsic redox activity with catecholase-like catalytic behavior (k_cat = 3.11 × 10⁶ s^- 1), suggesting a potential role in electron-transfer processes relevant to oxidative stress modulation. Overall, bacteriogenic SeNPs mitigate Cr(VI) toxicity through a combination of reduced metal uptake, altered intracellular metal dynamics, and attenuation of reactive oxygen species. These findings highlight the bioinorganic functionality of SeNPs as redox-active nanomaterials influencing metal-plant interactions and oxidative stress pathways.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Granaticin biosynthesis and engineering: mechanistic understanding, production bottlenecks, and computational opportunities.","authors":"Chang Chen, Bingyu Zhao, Qiaolei Zhu, Xiaodi Wang, Nairu Ji, Yunping Zhu","doi":"10.1007/s11274-026-05269-2","DOIUrl":"10.1007/s11274-026-05269-2","url":null,"abstract":"<p><p>Granaticin, as a member of the benzoisochromanequinone (BIQ) family, exhibits a range of biological activities, including antibacterial, antitumor, and antibiofilm effects. Notably, this compound displays a bright blue color under alkaline conditions, highlighting its potential application as a functional pigment.Although its biosynthetic gene cluster has been identified and partially characterized, efficient production of granaticin remains significantly constrained by an incomplete understanding of pathway regulation, enzymatic specificity, and metabolic flux distribution. Despite these advances, strategies for improving granaticin yield still rely largely on empirical approaches and lack predictive capability within complex biosynthetic systems. With the advancement of computational approaches-such as protein structure prediction, metabolic flux analysis, and data-driven optimization of fermentation processes-granaticin research is shifting from empirical optimization toward system-level and predictive engineering.This review systematically summarizes recent progress in granaticin research, with particular emphasis on biosynthetic mechanisms, pathway regulation, metabolic engineering strategies, tailoring enzyme functions, heterologous expression systems, and emerging computational approaches. In addition, the limitations of current engineering strategies and the major challenges associated with achieving scalable and controllable production are critically discussed.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated genomic and experimental assessment of the biosafety of Paraburkholderia sacchari as a promising microbial chassis.","authors":"Matheus Marques Torres, João Henrique Diniz Gervasio, Eric André Velasco Yépez, Amanda Silva Santos, Gabriele Muniz Costa, Maria Luisa Zardo, Juliana Cardinali-Rezende, Welington Luiz de Araújo, José Gregório Cabrera Gomez, Luiziana Ferreira da Silva","doi":"10.1007/s11274-026-05227-y","DOIUrl":"10.1007/s11274-026-05227-y","url":null,"abstract":"<p><p>Paraburkholderia sacchari is an environmental member of the Burkholderiaceae with recognized biotechnological potential, yet its genomic diversity, biosafety profile, and evolutionary relationship to pathogenic Burkholderia species remain poorly understood. Here, we present a comprehensive genomic and phenotypic characterization of P. sacchari strain LMG 19,450^T and compare it with three additional P. sacchari genomes and phylogenetically related Burkholderia species. A high-quality genome assembly confirmed its placement within the Paraburkholderia clade and species-level relatedness among P. sacchari strains despite detectable intraspecies genomic variability. Pangenome analysis revealed a large, conserved core genome and an extensive accessory gene repertoire, consistent with adaptation to plant-associated soil environments. Comparative analysis of virulence-associated genes showed a marked contrast between P. sacchari and pathogenic Burkholderia, with the former lacking most canonical pathogenicity determinants. Gene tree-species tree reconciliation further indicated a constrained horizontal gene transfer profile for virulence-associated gene families, while genes involved in transcriptional regulation displayed higher transfer propensity. Antimicrobial resistance profiling revealed a limited resistome dominated by efflux-associated homologs, consistent with broad phenotypic susceptibility. Notably, strain LMG 19,450^T exhibited antibiotic susceptibility patterns comparable to those of the established microbial chassis Pseudomonas putida KT2440. Virulence assessment using the Galleria mellonella model demonstrated markedly attenuated pathogenicity relative to Burkholderia cepacia. Together, these findings support P. sacchari as an environmentally adapted bacterium with a favorable biosafety profile and highlight the value of integrating genomic and experimental approaches to evaluate emerging microbial chassis.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}