World journal of microbiology & biotechnology最新文献_第8页

Advancing wound healing: controlled release of tannic acid via epitope imprinted antimicrobial spongy cover material.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-04 DOI: 10.1007/s11274-025-04266-1

Büşra Tuna, Pırıl Arısoy, Hatice İmge Oktay Başeğmez, Gözde Baydemir Peşint

{"title":"Advancing wound healing: controlled release of tannic acid via epitope imprinted antimicrobial spongy cover material.","authors":"Büşra Tuna, Pırıl Arısoy, Hatice İmge Oktay Başeğmez, Gözde Baydemir Peşint","doi":"10.1007/s11274-025-04266-1","DOIUrl":"10.1007/s11274-025-04266-1","url":null,"abstract":"The increasing resistance of microorganisms to conventional antibiotics calls for alternative antimicrobial strategies. This study introduces a novel approach to acute wound healing by incorporating epitope-imprinted spongy cover materials with antimicrobial properties, using Tannic acid (TA) as the active agent within biocompatible cryogels imprinted with gallic acid. The spongy materials were synthesized and characterized through Fourier Transform Infrared Spectroscopy (FTIR), swelling tests, and Scanning Electron Microscopy (SEM) to assess their structural and physicochemical properties. The antimicrobial efficacy of the cryogels, loaded with 1.5, 3, 5 mg/mL of TA concentrations, was tested against Staphylococcus aureus and Escherichia coli, common pathogens in wound infections. The highest inhibition zone was determined to be 15 mm for S. aureus and 12 mm for E. coli. Maximum TA adsorption was 210.27 mg/g for eMIP and 24.74 mg/g for NIP. Cumulative release studies revealed the highest release rate occurred within the first 2 h. TA release kinetics indicated a non-Fickian diffusion mechanism. Additionally, the biocompatibility and potential cytotoxicity of the spongy materials, including TA-loaded variants, were assessed using the MTT assay on cultured cells. The results confirmed that the spongy materials are non-toxic and do not inhibit cell proliferation, supporting their suitability for acute wound healing. This study demonstrates that TA-loaded epitope-imprinted Poly(2-hydroxyethyl methacrylate) (pHEMA)-based spongy materials possess antimicrobial properties, making them potential candidates for wound and burn dressing applications.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"59"},"PeriodicalIF":4.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11790693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient secretory expression, purification, and characterization of lipase in Pseudomonas aeruginosa M18, with multifunctional applications in diagnostics.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-31 DOI: 10.1007/s11274-025-04279-w

Yunbo Liu, Pan Zhu, Lingyuan Kong, Jing Wang, Chengdong Ji, Yuanli Li, Lichun Dong, Weijing Yi

{"title":"Efficient secretory expression, purification, and characterization of lipase in Pseudomonas aeruginosa M18, with multifunctional applications in diagnostics.","authors":"Yunbo Liu, Pan Zhu, Lingyuan Kong, Jing Wang, Chengdong Ji, Yuanli Li, Lichun Dong, Weijing Yi","doi":"10.1007/s11274-025-04279-w","DOIUrl":"10.1007/s11274-025-04279-w","url":null,"abstract":"Lipase (EC 3.1.1.3) is a crucial hydrolase with broad industrial and clinical applications. In this study, the lipA and lipH genes from Pseudomonas aeruginosa were cloned into the pBBR1MCS-2 vector and expressed under the regulation of the highly efficient BSFP_0720 promoter from Burkholderia stabilis FERMP-21014. This system allowed for efficient secretory expression in Pseudomonas aeruginosa without requiring an inducer. The recombinant lipase exhibited both lipase and cholesteryl esterase activities, making it suitable for triglyceride and cholesterol assay kits. Additionally, gene editing was used to knock out the endogenous cholesterol oxidase gene in Pseudomonas aeruginosa, eliminating cross-interference in different assay kits. High-density fermentation using glucose as the carbon source resulted in lipase activity reaching 68 kU/L and cholesteryl esterase activity reaching 214 kU/L after 30 h of fermentation, representing a 356-fold increase compared to natural production. By combining ammonium sulfate precipitation, hydrophobic interaction chromatography, and anion exchange chromatography, a purity of 94.32% was achieved (as determined by CE-SDS). Accelerated stability tests showed that the lyophilized lipase retained over 96% residual activity after storage at 37 °C for 21 days and at 45 °C for 7 days, suggesting its suitability for long-term storage. The enzymatic properties of the lipase demonstrated resistance to common chemicals, high activity in buffers with pH values between 7 and 9, and short-term tolerance to high temperatures (60 °C). These characteristics make the lipase highly adaptable for use in complex clinical samples and various industrial applications. The successful high-efficiency expression and multifunctional utility of this lipase highlight its significant commercial potential in diagnostics and other fields.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"57"},"PeriodicalIF":4.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical optimisation and analysis of biomass and exopolysaccharide production by Lacticaseibacillus rhamnosus LRH30.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-31 DOI: 10.1007/s11274-025-04273-2

Helena Mylise Copeland, Susan Maye, George MacLeod, Dermot Brabazon, Christine Loscher, Brian Freeland

{"title":"Statistical optimisation and analysis of biomass and exopolysaccharide production by Lacticaseibacillus rhamnosus LRH30.","authors":"Helena Mylise Copeland, Susan Maye, George MacLeod, Dermot Brabazon, Christine Loscher, Brian Freeland","doi":"10.1007/s11274-025-04273-2","DOIUrl":"10.1007/s11274-025-04273-2","url":null,"abstract":"Exopolysaccharides (EPS) produced by lactic acid bacteria with immunomodulatory potential are promising natural food additives. This study employs small-scale, 250 mL bioreactors combined with a central composite design to optimise two important bioprocess parameters, namely temperature and airflow, to achieve high yields of biomass and EPS from Lacticaseibacillus rhamnosus LRH30 (L. rhamnosus LRH30). A quadratic model was determined to be the best fit for the production of both products. The optimum critical process parameters for maximised biomass were identified to be 37.01 °C with an airflow of 0.12 vvm, while optimum criteria was 20.1 °C with an airflow of 0.18 vvm for maximum EPS production. Under these optimized conditions, small-scale batch experiments yielded a biomass concentration of 10.1 g/L and an EPS yield of 520.2 mg/L. In comparison, scale-up experiments in 2L reactors resulted in a biomass concentration of 8.54 g/L (a reduction of 18%) and an EPS yield of 654.6 mg/L (an increase of 26%). The produced EPS was purified and characterised through Fourier transform infrared spectroscopy and showed characteristic peaks associated with polysaccharides. The immunomodulatory potential of the L. rhamnosus LRH30 cells and EPS was evaluated through cytokine and chemokine secretion in a J774A.1 murine macrophage, resulting in a predominantly anti-inflammatory effect of L. rhamnosus LRH30 and EPS.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"58"},"PeriodicalIF":4.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of glycosylation in bacterial resistance to carbapenems.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-30 DOI: 10.1007/s11274-025-04272-3

Jing Dang, Xinlu Yu, Mengyuan Zhang, Liuyi Dang, Zheng Li, Jian Shu

{"title":"Role of glycosylation in bacterial resistance to carbapenems.","authors":"Jing Dang, Xinlu Yu, Mengyuan Zhang, Liuyi Dang, Zheng Li, Jian Shu","doi":"10.1007/s11274-025-04272-3","DOIUrl":"10.1007/s11274-025-04272-3","url":null,"abstract":"Carbapenems are a class of β-lactam antibacterial drugs with a broad antibacterial spectrum and strong activity, commonly used to treat serious bacterial infections. However, improper or excessive use of carbapenems can lead to increased bacterial resistance, which is a significant concern as they are often used as last resort for treating multidrug-resistant (MDR) gram-negative bacteria. Confronted with this challenge, it is crucial to comprehensively understand the mechanism of carbapenem resistance to develop effective therapeutic strategies and innovative drugs. In recent years, emerging research on the glycosylation of bacterial proteins has highlighted the crucial role of glycans in various bacterial processes, including carbapenem resistance. Given the limited understanding of bacterial glycosylation, its role in in carbapenem resistance may be more pivotal than currently acknowledged. In this review, we summarize the direct and multifunctional role of glycosylation in bacterial resistance as well as the classical and recently reported mechanisms of bacterial carbapenem resistance, focusing on illuminating the potential role of glycosylation in carbapenem resistance. We also discuss the potential of leveraging this knowledge to develop more effective strategies for combating clinically resistant bacteria.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"55"},"PeriodicalIF":4.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and functional characterization of sesquiterpene synthase genes from Inonotus obliquus using a Saccharomyces cerevisiae expression system.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-30 DOI: 10.1007/s11274-025-04274-1

Qiao Liu, Junzhi Peng, Ziling Tao, Jiluan Zhang, Weifan Wu, Zhiwu Tan, Tao Zhou, Xiaoying Cao, Jihong Jiang

{"title":"Cloning and functional characterization of sesquiterpene synthase genes from Inonotus obliquus using a Saccharomyces cerevisiae expression system.","authors":"Qiao Liu, Junzhi Peng, Ziling Tao, Jiluan Zhang, Weifan Wu, Zhiwu Tan, Tao Zhou, Xiaoying Cao, Jihong Jiang","doi":"10.1007/s11274-025-04274-1","DOIUrl":"10.1007/s11274-025-04274-1","url":null,"abstract":"Inonotus obliquus (Chaga mushroom) is a large medicinal and edible fungus that contains a wealth of bioactive terpenoids. However, the detection of certain low-abundance sesquiterpenoids remains a challenge due to limitations in extraction and analytical techniques. Furthermore, the synthase genes responsible for the biosynthesis of the identified terpenoids have not yet been clearly elucidated. To address this, our study combined transcriptome mining with yeast heterologous expression to investigate the synthase genes involved in sesquiterpenoid production in I. obliquus. We successfully identified eight sesquiterpene synthase genes and one farnesyltransferase. Among these, only cis-β-farnesene, synthesized by IoTPS2, had been previously detected before in the sclerotium of I. obliquus, while the other nine sesquiterpenoids-including neoisolongifolene-8-ol, β-longipinene, vetiselinenol, isolongifolene, 7,8-dehydro-8a-hydroxy-, 4a,8b,10b,11a-tetramethylbicyclo[6.3.0]undec-1-en-5-one, 6,11-oxido-acor-4-ene, β-maaliene, neointermedeol, and longifolenaldehyde-were discovered for the first time. This research provides a critical scientific foundation for expanding the known repertoire of sesquiterpenoids and their corresponding synthase genes in I. obliquus.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"56"},"PeriodicalIF":4.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current insights into molecular mechanisms of environmental stress tolerance in Cyanobacteria.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-29 DOI: 10.1007/s11274-025-04260-7

Preeti Rai, Ruchi Pathania, Namrata Bhagat, Riya Bongirwar, Pratyoosh Shukla, Shireesh Srivastava

{"title":"Current insights into molecular mechanisms of environmental stress tolerance in Cyanobacteria.","authors":"Preeti Rai, Ruchi Pathania, Namrata Bhagat, Riya Bongirwar, Pratyoosh Shukla, Shireesh Srivastava","doi":"10.1007/s11274-025-04260-7","DOIUrl":"10.1007/s11274-025-04260-7","url":null,"abstract":"The photoautotrophic nature of cyanobacteria, coupled with their fast growth and relative ease of genetic manipulation, makes these microorganisms very promising factories for the sustainable production of bio-products from atmospheric carbon dioxide. However, both in nature and in cultivation, cyanobacteria go through different abiotic stresses such as high light (HL) stress, heavy metal stress, nutrient limitation, heat stress, salt stress, oxidative stress, and alcohol stress. In recent years, significant improvement has been made in identifying the stress-responsive genes and the linked pathways in cyanobacteria and developing genome editing tools for their manipulation. Metabolic pathways play an important role in stress tolerance; their modification is also a very promising approach to adapting to stress conditions. Several synthetic as well as systems biology approaches have been developed to identify and manipulate genes regulating cellular responses under different stresses. In this review, we summarize the impact of different stresses on metabolic processes, the small RNAs, genes and heat shock proteins (HSPs) involved, changes in the metabolome and their adaptive mechanisms. The developing knowledge of the adaptive behaviour of cyanobacteria may also be utilised to develop better stress-responsive strains for various applications.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"53"},"PeriodicalIF":4.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and structure determination of a new antibacterial lanthipeptide derived from the marine derived bacterium Lysinibacillus sp.CTST325.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-29 DOI: 10.1007/s11274-024-04212-7

Chanaphat Thetsana, Ryota Moriuchi, Shinya Kodani

引用次数: 0

The legacy of endophytes for the formation of bioactive agents, pigments, biofertilizers, nanoparticles and bioremediation of environment.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-28 DOI: 10.1007/s11274-025-04265-2

Anjali Panwar, Suvendu Manna, Gayatri Sahini, Vivek Kaushik, Manoj Kumar, Muthusamy Govarthanan

{"title":"The legacy of endophytes for the formation of bioactive agents, pigments, biofertilizers, nanoparticles and bioremediation of environment.","authors":"Anjali Panwar, Suvendu Manna, Gayatri Sahini, Vivek Kaushik, Manoj Kumar, Muthusamy Govarthanan","doi":"10.1007/s11274-025-04265-2","DOIUrl":"10.1007/s11274-025-04265-2","url":null,"abstract":"Endophytes have significant prospects for applications beyond their existing utilization in agriculture and the natural sciences. They form an endosymbiotic relationship with plants by colonizing the root tissues without detrimental effects. These endophytes comprise several microorganisms, including bacteria and fungi. They act as repositories of compounds of medicinal importance. They are considered sources of pigments besides synthetic dyes and assist with soil fertility and plant growth as bio-fertilizers. They also have immense potential for advanced technology using endophyte-synthesized nanoparticles. In assisting bioremediation, they facilitate detoxification of pollutants in all spheres of the environment. Studies on the potential of endophytic microbes in drug discovery and biotic stress management are underway. In this review, published databases on endophytes and their diverse roles and applications in various fields, such as bio-fertilizers and nanoparticles, as well as bioremediation, are critically discussed while exploring unanswered questions. In addition, future perspectives on endosymbiotic microorganisms and their prospective use in plants, environmental management, and medicine are discussed in this review.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"52"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal lignocellulolytic enzymes: an in silico and full factorial design approach.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-27 DOI: 10.1007/s11274-024-04241-2

Esteffany de Souza Candeo, Fabiano Bisinella Scheufele, Aline de Cassia Campos Pena, Gabriel Dequigiovanni, Giani Andrea Linde, Gerardo Mata, Nelson Barros Colauto, Patricia Dayane Carvalho Schaker

{"title":"Fungal lignocellulolytic enzymes: an in silico and full factorial design approach.","authors":"Esteffany de Souza Candeo, Fabiano Bisinella Scheufele, Aline de Cassia Campos Pena, Gabriel Dequigiovanni, Giani Andrea Linde, Gerardo Mata, Nelson Barros Colauto, Patricia Dayane Carvalho Schaker","doi":"10.1007/s11274-024-04241-2","DOIUrl":"10.1007/s11274-024-04241-2","url":null,"abstract":"Efficient degradation of lignocellulosic biomass is key for the production of value-added products, contributing to sustainable and renewable solutions. This study employs a two-step approach to evaluate lignocellulolytic enzymes of Ceratocystis paradoxa, Colletotrichum falcatum, and Sporisorium scitamineum. First, an in silico genomic analysis was conducted to predict the potential enzyme groups produced by these fungi. Second, a 2³ full factorial design of solid-state cultivation was employed to investigate the cultivation conditions that optimize enzyme activity. In silico analysis of phytopathogen genomes identified proteins with the potential for biomass degradation. Cellulase and phenoloxidase activities were assessed in culture medium and solid-state cultivation. A 2³ full factorial design was employed for solid-state cultivation to evaluate the cellulose, endoglucanase, and laccase activities. In silico analysis shows that C. falcatum has the most diverse enzyme set for lignocellulosic biomass degradation. In vitro assays corroborate this, demonstrating that C. falcatum produces the highest enzyme quantities, except for cellulase, where C. paradoxa outperforms it. Both C. paradoxa and C. falcatum exhibit cellulase and phenoloxidase activities, but only C. falcatum shows laccase activity. Most favorable enzyme production in solid-state cultivation occurred with 85-95 g 100 g- 1 bagasse moisture and 5 g 100 g- 1 yeast extract, with four-day cultivation period needed for cellulase and endoglucanase in C. paradoxa and 12 days for endoglucanase and laccase in C. falcatum. The in silico and in vitro assays demonstrated that C. falcatum can produce a diverse enzyme set, including laccase, cellulase, and endoglucanase, making it a promising candidate for enzymatic industrial applications.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"50"},"PeriodicalIF":4.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fitness and adaptive evolution of a Rhodococcus sp. harboring dioxin-catabolic plasmids.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-27 DOI: 10.1007/s11274-025-04270-5

Xu Wang, Changai Fu, Meng Chen, Yanan Wu, Yu Chen, Yan Chen, Li Li

{"title":"Fitness and adaptive evolution of a Rhodococcus sp. harboring dioxin-catabolic plasmids.","authors":"Xu Wang, Changai Fu, Meng Chen, Yanan Wu, Yu Chen, Yan Chen, Li Li","doi":"10.1007/s11274-025-04270-5","DOIUrl":"10.1007/s11274-025-04270-5","url":null,"abstract":"Catabolic plasmids are critical factors in the degradation of recalcitrant xenobiotics, such as dioxins. Understanding the persistence and evolution of native catabolic plasmids is pivotal for controlling their function in microbial remediation. Here, we track the fitness and evolution of Rhodococcus sp. strain p52 harboring dioxin-catabolic plasmids under nonselective conditions without contaminant. Growth curve analysis and competition experiments demonstrated that pDF01 imposed fitness costs, whereas pDF02 conferred fitness benefits. During stability tests, pDF01 tended to be lost from the population, while pDF02 maintained at least one copy in the cell until proliferation of the 400th generation. Genome-wide gene expression profiling combined with codon usage bias analysis revealed that the high expression of pDF01 genes involved in dibenzofuran catabolism and regulation caused metabolic burdens. In contrast, potential cooperation between the pDF02-encoded short-chain dehydrogenase/reductase family oxidoreductase and the redox cofactor mycofactocin, which synthetic genes are located on the chromosome, may explain the benefit of pDF02. The fitness cost imposed by pDF01 was alleviated during adaptive evolution and was associated with the transcriptional downregulation of the dibenzofuran degradation genes on pDF01, and the global regulation of genome-wide gene expression involving basic metabolism, transport, and signal transduction. This study broadens our understandings on the persistence and evolution of dioxin-catabolic mega-plasmids, thus paving the way for the bioremediation of recalcitrant xenobiotic pollution in the environment.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"51"},"PeriodicalIF":4.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0