World journal of microbiology & biotechnology最新文献

{"title":"Exploring the structural landscape of universal stress proteins in Archaea.","authors":"L Matarredona, M J García-Bonete, B Zafrilla, J Esclapez","doi":"10.1007/s11274-025-04439-y","DOIUrl":"10.1007/s11274-025-04439-y","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"196"},"PeriodicalIF":4.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laccase-catalyzed conversion of residual agricultural biomass to lignin-derived aromatic compounds.","authors":"Laura E Navas, Ornella M Ontañon, Juliana Topalian, Lucy L Coria-Oriundo, Sonia Wirth, Leonardo D Gomez, Lindsay D Eltis, Eleonora Campos","doi":"10.1007/s11274-025-04440-5","DOIUrl":"10.1007/s11274-025-04440-5","url":null,"abstract":"<p><p>Lignin, one of the three major components of lignocellulosic biomass, is a promising feedstock for value-added bioproducts. However, its utilization is limited by its intrinsic heterogeneity and recalcitrance. Laccases are oxidoreductases that can act on a wide range of phenolic and aromatic compounds. SilA is a small robust thermostable laccase from the actinobacterium Streptomyces ipomoea that is active across a broad range of reaction conditions. In this study, we demonstrated that SilA transforms the lignin of sugar cane straw residue that has been pretreated by steam explosion (SCR_SE), into lignin-derived aromatic compounds (LDACs), specifically 4-hydroxybenzoate, vanillate, syringate, p-coumarate and ferulate, which are in great demand across diverse industries, including chemical, pharmaceutical, food, health, and cosmetic. Additionally, incubation of SCR_SE with SilA also produced acid-precipitable polymeric lignin (APPL), as an indirect indication of delignification. Finally, when the major fraction of SCR_SE polysaccharides was removed by the commercial enzymatic cocktail Cellic^® CTec2, there was a significant increase in the release of LDACs, particularly p-coumarate. This research demonstrates a novel biocatalytic approach to transform lignin from the main residual solid waste stream of lignocellulosic biomass after the hydrolysis of most of the structural polysaccharides, particularly in the context of sugar cane biorefineries, contributing to the sustainable and efficient use of agricultural biomass waste.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"197"},"PeriodicalIF":4.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome wide analysis of Priestia aryabhattai_OP, an endobacterium, modulating growth, development and biochemical compositions of sporophores in edible oyster mushroom Pleurotus ostreatus (MTCC 1802).","authors":"Chandana Paul, Tina Roy, Madhurima Roy, Athira C Rajeev, Archana Pan, Madhumita Maitra, Nirmalendu Das","doi":"10.1007/s11274-025-04438-z","DOIUrl":"10.1007/s11274-025-04438-z","url":null,"abstract":"<p><p>The increasing global interest in the consuming and producing of edible oyster mushrooms (Pleurotus spp.) is driven by their well-documented nutritional and health benefits. The metagenomic analysis of fruiting body revealed a distinct microbial composition in P. ostreatus, predominantly comprising Pseudomonodota (~ 82%) and Bacillota (~ 10%). An endobacterium Priestia aryabhattai_OP, associated with internal tissue of P. ostreatus (MTCC 1802), was isolated and characterized through biochemical and microscopic analyses as well as 16 S rRNA and whole genome sequencing. Co-cultivation of P. ostreatus with this bacterium significantly enhanced the in vitro production of laccase, a key growth-promoting enzyme. Additionally, the endobacterium improved the biological efficiency (BE) of the mushroom, enriched its nutraceutical profile, and facilitated the biosynthesis of beneficial compounds, including IAA, siderophores, and antimicrobials like lassopeptides, phosphonates, non-ribosomal iron-binding siderophores (NI- siderophore), carotenoids, paeninodins, synechobactins, and surfactins. The present findings offer novel insights into microbe-microbe interactions and their pivotal roles in fungal biology, with significant implications for sustainable mushroom production as well as nutrient enrichment and biotechnological advancements.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"194"},"PeriodicalIF":4.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic and enzymatic characterization of linoleic acid biotransformation by Lactiplantibacillus plantarum NGML2 to conjugated linoleic acid and different metabolites.","authors":"Jasra Naseeb, Munazza Kanwal, Sam Aldalali, Abid Sarwar, Syed Babar Jamal, Zhennai Yang, Tariq Aziz, Maha Alharbi, Ashwag Shami, Fahad Al-Asmari, Hanan Abdulrahman Sagini, Fakhria A Al-Joufi","doi":"10.1007/s11274-025-04420-9","DOIUrl":"10.1007/s11274-025-04420-9","url":null,"abstract":"<p><p>Biotransformation is a biological process in which microorganisms or enzymes convert a substrate into a different chemical compound, often resulting in bioactive metabolites with therapeutic or industrial applications. Linoleic acid (LA) is a polyunsaturated fatty acid that has gained attention for its potential health benefits, including its role in modulating inflammation and oxidative stress. The ability of microorganisms to biotransform LA into bioactive metabolites, such as conjugated linoleic acid (CLA) isomers, provides an opportunity for the development of functional foods and nutraceuticals. This study aimed to explore the metabolic capacity of Lactiplantibacillus plantarum NGML2 to biotransform LA into bioactive compounds, assess the effect of varying LA concentrations on metabolite production, and investigate the molecular mechanisms underlying the process using in-silico tools. In vitro experiments were conducted to determine the production of conjugated LA and LA analogues by L. plantarum NGML2 at different concentrations of LA (2%, 3%, 4%, 6%, and 10%). A total of 19 metabolites were identified, including two conjugated LA isomers-10E,12Z-octadecadienoic acid and (9E,11E)-octadecadienoic acid-produced in concentrations up to 504.31 mg/L and 228.88 mg/L, respectively. Fifteen LA analogues, along with phenolic and alcoholic metabolites, were also synthesized, with the highest concentrations observed at moderate LA levels (4%). In-silico enzyme analysis identified key enzymes involved in LA biotransformation, including epoxide hydrolase and 6-phosphogluconolactonase, with molecular docking revealing strong binding affinities (- 8.9 kJ/mol) for LA derivatives. Molecular dynamics simulations further corroborated these findings, demonstrating stable interactions between LA and these enzymes. This study underscores the potential of L. plantarum NGML2 in the biotransformation of LA into valuable metabolites. The results lay the groundwork for developing microbial strains for industrial-scale production of bioactive lipids. Future research should focus on optimizing biotransformation pathways and expanding the range of bioactive compounds produced for use in functional food and nutraceutical industries.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"193"},"PeriodicalIF":4.0,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of brucellosis by a two-signal vertical flow immunoassay based on sea urchin-like gold nanoparticles.","authors":"Chao Tong, Tongtong Zhang, Zhenghong Xu, Min Zhang, Zhihua Xu, Yu Ma, Zhihan Niu, Feng Shi","doi":"10.1007/s11274-025-04409-4","DOIUrl":"10.1007/s11274-025-04409-4","url":null,"abstract":"<p><p>Brucellosis, a chronic zoonotic disease caused by Brucella, poses a significant threat to global public health and safety. While vertical flow immunoassay (VFI) is an effective tool for detecting brucellosis, its low sensitivity and poor quantitative ability significantly limit its application in brucellosis detection. Therefore, in this study, a dual-mode signal output technique was designed for rapid detection of brucellosis using the photothermal properties of sea urchin-like gold particles (ULGNs). ULGNs were prepared using the hydroquinone reduction method, and their localized surface plasmon resonance (LSPR) peaks could be easily tuned to the near-infrared (NIR) range by controlling the surface spikes. Subsequently, the ULGNs were detected using a Staphylococcus aureus protein A(SPA) probe coupled to the ULGNs. The colorimetric signal, observed by the naked eye, could show the results within 10 min, and its minimum detection limit was 2 IU mL^- 1. In addition, the ULGNs, irradiated at 808 nm, increased the temperature rapidly. Detecting the temperature difference at the T-points, with the minimum detection limit of 0.8 IU mL^- 1, which was 2.5-fold amplified compared with the colorimetric signal, enabled the rapid output of the photothermal signals. In addition, based on the temperature change of the T-point, it is possible to preliminarily quantify the antibody level in the patient's body, providing a rapid preventive effect. Therefore, ULGNs-VFI effectively improved the detection sensitivity and quantitative accuracy. The method has no cross-reactivity with human S. aureus, sheep enterococcus faecalis, human mycobacterium tuberculosis-positive serum samples, as well as rabbit E. coli, and has exceptional specificity and stability. Therefore, ULGNs-VFI has the potential for practical application in brucellosis POCT detection.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"192"},"PeriodicalIF":4.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant growth-promoting Burkholderia Sp. ZF6 enhances Brassica juncea survival in Cd/Zn contaminated soils via heavy metal tolerance genes.","authors":"Soo Yeon Lee, Kyung-Suk Cho","doi":"10.1007/s11274-025-04429-0","DOIUrl":"10.1007/s11274-025-04429-0","url":null,"abstract":"<p><p>In this study, we focused on Burkholderia sp. ZF6, a promising plant growth-promoting bacterium (PGPB) known for its robust heavy metal tolerance, enduring 5 mM cadmium (Cd) and 50 mM zinc (Zn), and carrying the tolerance genes cadA and zntA. ZF6 showed outstanding plant growth-promoting (PGP) abilities, including nitrogen fixation (OD₆₃₀ = 0.331 ± 0.012), over 50% DPPH radical scavenging activity, high levels of indole-3-acetic acid (IAA) production, phosphatase activity, and siderophore production. For the experimental trials, ZF6 was used to inoculate Indian mustard (Brassica juncea) seeds in multi-metal-contaminated soil (smelter soil), resulting in a 1.4-fold increase in seed germination. Subsequent pot experiments with soil co-contaminated with 100 mg Cd·kg^- 1 and 2,000 mg Zn·kg^- 1 demonstrated a 1.5- and 1.6-fold increase in shoot and root lengths, respectively, a 2.1-fold boost in vigor index, and a 1.1- to 1.2-fold rise in plant biomass. Over the experiment period, the relative abundance of cadA and zntA genes increased by 1.6- and 18.4-fold, respectively. These findings demonstrate the potential of Burkholderia sp. ZF6 as a biofertilizer that enhances heavy metal tolerance and promotes Indian mustard growth in highly contaminated soil while improving overall soil PGP activity.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"191"},"PeriodicalIF":4.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable exploitation of high-protein feather waste for green production of cold-adapted and detergent-stable keratinase by Penicillium oxalicum AUMC 15084.","authors":"Ayat M A Abdel-Latif, Nageh F Abo-Dahab, Ahmed M Moharram, Abdallah M A Hassane, Osama A M Al-Bedak","doi":"10.1007/s11274-025-04417-4","DOIUrl":"10.1007/s11274-025-04417-4","url":null,"abstract":"<p><p>Limited research has investigated the ability of psychrophilic and psychro-tolerant microorganisms to produce cold-active keratinases, despite their potential as an efficient alternative for substrate conversion at reduced energy expenditure. A screening of 32 Penicillium and Talaromyces isolates for keratinolytic activity at temperatures of 5, 10, and 15ºC identified a promising P. oxalicum strain as the most potent at 10ºC, yielding 242.39 U/mL. Following six days of incubation at pH 8.0 and 15 °C with 0.2% yeast extract as the nitrogen source, the P. oxalicum strain exhibited keratinase activity of 359.42 U/mL. The keratinase underwent purification with a 4.13-fold increase, utilizing an MP 800 anion exchanger and Sephacryl S 200 , resulting in a specific activity of 684.46 U/mg and a yield of 5.34%. The SDS-PAGE analysis identified a keratinase with a molecular weight of 37.51 kD, exhibiting peak activity at pH 9.0 and 20ºC, with a specific activity of 721.8 U/mg. Mg²⁺, Zn²⁺, and Mn²⁺ enhanced keratinase activity by 156.0%, 140.60%, and 156.0%, respectively. The keratinase activity was significantly enhanced (p < 0.05) by the addition of 5 mM SDS (139.15%), 5 and 10% mercaptoethanol (1125.70 and 1327.0%, respectively), and 5 and 10% DMSO (128.30 and 227.40%, respectively). The dehairing potential of P. oxalicum AUMC 15084, utilizing crude keratinase on goat skin, demonstrated complete dehairing after 20 h at 20ºC with the crude preparation. This study provides a promising Penicillium oxalicum strain that could be used for production of cold-active keratinase. The effectiveness of the produced keratinase in the dehairing process was demonstrated as an environmentally friendly alternative to the traditional chemical procedure.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"190"},"PeriodicalIF":4.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cardamom-Fusarium pathosystem: current knowledge and future directions.","authors":"Sandra Tomichen, Shweta Panchal","doi":"10.1007/s11274-025-04426-3","DOIUrl":"10.1007/s11274-025-04426-3","url":null,"abstract":"<p><p>Small cardamom (Elettaria cardamomum), known as the \"Queen of Spices,\" is severely affected by rot diseases caused by Fusarium oxysporum Schlecht, leading to significant crop losses globally. This review provides an overview about the plant, its pathogen F. oxysporum and the biocontrol agents used. The pathogenesis of the fungus, the host defence mechanisms and the mechanisms used by biocontrol agents like Bacillus subtilis, Pseudomonas fluorescens, Trichoderma viride, Trichoderma harzianum, and Arbuscular Mycorrhizal Fungi (AMF) are discussed. F. oxysporum is a globally recognized pathogen responsible for wilting diseases in a wide range of economically important plants, including tomato, watermelon, asparagus, potato, banana, cucumber, pepper, chickpea, cotton, and more. Additionally, it is known to cause rot disease in cardamom. This review also recognizes the lacunae in plant pathology research in this pathosystem and highlights future research approaches that are needed to improve our understanding of cardamom-Fusarium biology and to find effective biocontrols. At this point, it is imperative to support studies that examine the physiological and genetic responses of the organisms involved as well as extensive omic research to create a model of the interactions between cardamom, Fusarium, and the biocontrol agent. Development of specific techniques for microscopic visualization of the interactions and in vitro growth of cardamom seedlings will accelerate this research. These strategies hold potential for mitigating the devastating impact of F. oxysporum on cardamom cultivation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"189"},"PeriodicalIF":4.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration and heterologous expression of laccase genes and pesticide degradation ability of laccases from Cerrena unicolor GC.u01.","authors":"Jie Chu, Xiaoxiao Zhang, Ruihong Sun, Yuanqiang Lv, Zhuran Hu, Wenjuan Zhang, Xiaoran Shen, Yanhua Huang","doi":"10.1007/s11274-025-04435-2","DOIUrl":"10.1007/s11274-025-04435-2","url":null,"abstract":"<p><p>Laccases are valuable industrial enzymes with applications across various fields. While heterologous expression in Pichia pastoris is a common strategy, current approaches face limitations in yield, stability, and catalytic efficiency against recalcitrant agrochemicals. In this study, we sequenced and annotated the first high-quality genome of Cerrena unicolor strain GC.u01 (30.95 Mb, 8,089 genes), revealing a unique laccase gene family comprising nine members. Structural analysis revealed novel catalytic motifs in Lac2, which was successfully expressed in P. pastoris GS115 through codon optimization, yielding a novel recombinant enzyme (70 kDa) with exceptional pH stability (retaining > 80% activity at pH 3.0-8.0 for 24 h) and thermotolerance (> 60% activity at 40 °C), surpassing most reported fungal laccases. Notably, Lac2 demonstrated unprecedented degradation efficiency for azoxystrobin (96.2) and phoxim (30.7%)-the first report of a Cerrena unicolor laccase degrading these pesticides-achieving significantly higher rates than previously described laccases under similar conditions. This study integrates genome mining, enzyme engineering, and functional validation to establish a new paradigm for developing robust biocatalysts against recalcitrant agrochemicals. These unique characteristics of Lac2 suggest the potential of this enzyme in biotechnological and industrial applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"188"},"PeriodicalIF":4.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity analysis of municipal solid waste landfills soils of Delhi (NCR) and plastic dump sites of Uttar Pradesh region of India and their function prediction for plastic degrading enzymes.","authors":"Viral Kumar Tiwari, Akanksha Vishwakarma, Digvijay Verma, Monica Sharma","doi":"10.1007/s11274-025-04404-9","DOIUrl":"10.1007/s11274-025-04404-9","url":null,"abstract":"<p><p>Municipal solid waste landfills are rich in plastic waste, fostering a unique microbial ecosystem distinct from natural habitats. This study aimed to investigate the microbial diversity at four landfill and plastic dump sites using a 16S rDNA metataxanomics approach, and to predict their potential for plastic degradation. Environmental DNA was isolated and analyzed through Illumina MiSeq sequencing. The results revealed a dominant presence of Pseudomonadota (47%) across all sites, with key genera including Streptomyces, Galbibacter, Alcanivorax, and OM190. The most abundant species were unclassified Chloroflexi bacterium, Galbibacter marinus, Gracilimonas amylolytica, and Teredinibacter sp. Alpha diversity analysis showed the highest species richness in plastic dump site in Utrathia (Lucknow), followed by Ghazipur landfill Site (Delhi), with low evenness across the bacterial communities. Beta diversity analysis, using Bray-Curtis and PCA, indicated distinct microbial profiles for each site. The PICRUSt analysis identified 402 genes related to nine enzyme categories involved in plastic degradation. Pearson correlation network analysis of top 1% genera highlighted positive associations between genera like Galbibacter, Alcanivorax, Thioalkalimicrobium, Idiomarina and Pseudomonas with peroxygenase enzyme. This study underscores the microbial diversity and functional potential of landfill microbes in plastic degradation, contributing to our understanding of the plastisphere microbiome in landfill environments.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"187"},"PeriodicalIF":4.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}