World journal of microbiology & biotechnology最新文献_第10页

Quorum quenching of phenolic compounds from Leptolyngbya spp. MACC 32 via downregulation of the periplasmic receptor LuxP in Vibrio harveyi: Hinting a putative mechanism. 哈威弧菌MACC 32中酚类化合物的群体猝灭：通过下调质周受体LuxP提示一个假定的机制。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-27 DOI: 10.1007/s11274-025-04378-8

Madiyan Saranya, Byeol Ryu, Jose Seena, Bhaskaran Silpa, Ameer Ahna, C Benjamin Naman, Evgenia Glukhov, T P Sajeevan, I S Bright Singh, William H Gerwick, Joseph Valsamma

{"title":"Quorum quenching of phenolic compounds from Leptolyngbya spp. MACC 32 via downregulation of the periplasmic receptor LuxP in Vibrio harveyi: Hinting a putative mechanism.","authors":"Madiyan Saranya, Byeol Ryu, Jose Seena, Bhaskaran Silpa, Ameer Ahna, C Benjamin Naman, Evgenia Glukhov, T P Sajeevan, I S Bright Singh, William H Gerwick, Joseph Valsamma","doi":"10.1007/s11274-025-04378-8","DOIUrl":"10.1007/s11274-025-04378-8","url":null,"abstract":"Vibrio harveyi causes vibriosis, leading to high mortality and economic loss in global aquaculture. Quorum sensing (QS) driven biofilm formation makes them more resistant to various control measures. This study examined QS inhibition (QSI) of V. harveyi LB3 using marine cyanobacterial natural products. Qualitative and quantitative (88%) assays confirmed the quorum quenching (QQ) efficiency of the intracellular component of Leptolyngbya spp. MACC 32. The active fraction (E3) of the extract showed less than 50% toxicity to PmLyo-Sf9 cells and brine shrimp at 1 mg mL-1. At MIC (0.06 mg mL-1), E3 reduced biofilm biomass by 40-45% and metabolic activity by 10-20% without affecting bacterial growth. V. harveyi LB3 treated with E3 showed less gelatinase (64.75%), hemolytic activity (17.55%), motility (8-16%) and reduced EPS formation (45.5%), indicating its anti-virulence properties. HPLC, 1H NMR, and GC-MS analyses confirmed that E3 contains 4-hydroxybenzoic acid (4-HBA) and 4-hydroxybenzaldehyde (4-HBAL). Synthetic controls validated the QQ of 4-HBA and 4-HBAL. Molecular docking studies hinted at the putative interaction of 4-HBA and 4-HBAL with QS proteins (LuxR, LuxP, LuxM, LuxN, LuxQ, LuxU, LuxO and LuxS) of V. harveyi. Gene expression studies showed strong LuxR, LuxP, LuxQ, and ToxR downregulation (61-97%), differential upregulation of LuxO, LuxU, aphA, qrr1-qrr5 (9-18%), and LuxS, LuxM, LuxN, Fur (2-4%). Although not confirmed with mutant strains, this study provides strong evidence that phenolic compounds from Leptolyngbya spp. MACC 32 inhibited V. harveyi LB3 QS by blocking AI-2 binding to LuxP, supporting their role in shrimp health management through the control of vibriosis.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"233"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in microbial production and consumption of nitrous oxide in agricultural soils. 农业土壤中氧化亚氮微生物生产和消耗的最新进展。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-27 DOI: 10.1007/s11274-025-04464-x

Ruonan Xiong, Nan Gao, Weiqiang Huang, Xiaoyue Zhang, Weishou Shen

{"title":"Recent progress in microbial production and consumption of nitrous oxide in agricultural soils.","authors":"Ruonan Xiong, Nan Gao, Weiqiang Huang, Xiaoyue Zhang, Weishou Shen","doi":"10.1007/s11274-025-04464-x","DOIUrl":"10.1007/s11274-025-04464-x","url":null,"abstract":"Agricultural soil is a significant source of nitrous oxide (N2O), a long-lived greenhouse gas. Several microbial processes in the nitrogen cycle generate N2O but the only known sink for N2O in the biosphere is the reduction of N2O to N2 catalyzed by N2O reductase (NosZ). In this review, we summarized the latest knowledge on (i) key microbial pathways regulating N2O production and consumption processes in agricultural soils, including nitrification and denitrification and (ii) emerging strategies for microbial-mediated mitigation of N2O emissions from agricultural soils, including the use of nitrification and denitrification inhibitors, and the direct use of microorganisms to enhance NosZ activity. We focused on the screening and application strategies for microorganisms that can mitigate N2O emissions. We summarized two ecological mechanisms of microbial inoculation mitigation of N2O emissions from agricultural soils. One mechanism involved employing N2O-reducing microorganisms containing nosZ gene to directly mitigate N2O emissions from agricultural soils. The other mechanism utilized plant growth-promoting rhizobacteria to alter the community composition, abundance and activity of the N2O-producing or -reducing microorganisms and indirectly mitigate N2O emissions from agricultural soils. Additionally, we discussed the potential challenges affecting microbial inoculation technology, and explored its application prospects for reducing N2O emissions from agricultural soils. By providing a comprehensive overview of these topics, we aimed to effectively design and apply microbial-mediated mitigation technologies to better manage and mitigate N2O emissions from agricultural soils, ultimately contributing to global climate change mitigation efforts.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"235"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized solid lipid nanoparticles for co-delivery of gentamicin and doxycycline: a novel approach to combat intracellular brucella abortus infections. 优化固体脂质纳米颗粒共同递送庆大霉素和强力霉素：一种新的方法来对抗细胞内流产布鲁氏菌感染。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-27 DOI: 10.1007/s11274-025-04427-2

Bandar Almutairy, Sitah Alharthi, Hasan Ebrahimi Shahmabadi, Seyed Ebrahim Alavi

{"title":"Optimized solid lipid nanoparticles for co-delivery of gentamicin and doxycycline: a novel approach to combat intracellular brucella abortus infections.","authors":"Bandar Almutairy, Sitah Alharthi, Hasan Ebrahimi Shahmabadi, Seyed Ebrahim Alavi","doi":"10.1007/s11274-025-04427-2","DOIUrl":"10.1007/s11274-025-04427-2","url":null,"abstract":"This study evaluates the potential of gentamicin (GEN) and doxycycline (DOX) co-loaded solid lipid nanoparticles (SLNs) for treating Brucella abortus infections. Optimized SLN formulations demonstrated mean particle sizes of 211.2 ± 10.8 nm, a polydispersity index of 0.42 ± 0.019, encapsulation efficiency of 95.3 ± 1.2%, and loading efficiency of 14.7 ± 0.1%. Drug release profiles revealed a biphasic release, achieving 91.1% and 73% cumulative release for GEN and DOX, respectively, over 72 h. In vitro studies on J774.A1 macrophages indicated significantly enhanced antibacterial activity and reduced cytotoxicity for SLN-GEN/DOX, achieving a minimum inhibitory concentration of 1.25 µg/mL and reducing intracellular bacterial load to 3.7 ± 0.02 Log10 colony forming unit (CFU). In vivo, SLN-GEN/DOX-treated mice exhibited superior bacterial clearance, with spleen bacterial counts of 1.4 ± 0.03 Log10 CFU compared to 2.4 ± 0.06 Log10 CFU for the free drug combination. Additionally, reduced systemic toxicity was observed, with ALT and AST levels of 42.3 ± 1.2 U/mL and 64.1 ± 1.6 U/mL, respectively. These findings suggest that SLN-GEN/DOX offers a promising therapeutic approach for brucellosis by improving drug stability, bioavailability, and efficacy while minimizing systemic toxicity. Further studies should focus on clinical applications and mechanistic insights into SLN-mediated drug delivery.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"232"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multifaceted approach of Rhizobium sp. PS1 for biodegradation of azo dye blue GSL and plant growth promotion by alleviating dye induced stress. 根瘤菌PS1生物降解偶氮染料蓝GSL及缓解染料诱导胁迫促进植物生长的多方位研究

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04443-2

Mohini Pimpalse, Naga Rathna Supriya G

{"title":"A multifaceted approach of Rhizobium sp. PS1 for biodegradation of azo dye blue GSL and plant growth promotion by alleviating dye induced stress.","authors":"Mohini Pimpalse, Naga Rathna Supriya G","doi":"10.1007/s11274-025-04443-2","DOIUrl":"10.1007/s11274-025-04443-2","url":null,"abstract":"Current research has focused on bacteria which have bioremediation ability of textile dye along with plant growth promoting activities. Based on decolorization potential done by plate assay and broth assay, one isolate has the highest decolorization efficiency (91.56%) against Blue GSL azo dye (100 ppm) and identified as Rhizobium sp. PS1 (OP303241). Based on UV-Vis spectral analysis, bacterial treated dye showed a gradual decrease peak compared to untreated. The efficacy of biodegradation was confirmed by analytical methods such as TLC, FTIR, and HPLC, which confirmed the reduction of the azo bond. Rhizobium sp. PS1 exhibited multiple promote plant growth traits such as the nitrogen-fixing ability, solubilize phosphate, produce IAA, siderophores and gibberellin. The phytotoxicity and microtoxicity of the dye degraded products showed reduced toxicity compared to the control dye. Rhizobium sp. PS1 revealed the advantageous effects of on Vigna radiata L. (GM-6) with increased germination rates, physical parameters, and chlorophyll content under dye stress. Analysis of the antioxidant enzyme demonstrated the ability of the strain to mitigate the dye-induced oxidative stress, while the significant reduction of dye concentration in the soil highlights the remediation effectiveness. Overall, this study illustrates the dual capability of Rhizobium sp. PS1 for azo dye degradation and plant growth promotion, offering an eco-friendly approach to improve productivity in dye-contaminated land.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"210"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of phthalates DIBP, DMP, and DEP by Paenarthrobacter sp. strain PH1 - analysis of degradation, pathway, and its bioremediation potentiality in soil microcosm. Paenarthrobacter sp.菌株PH1对邻苯二甲酸酯DIBP、DMP和DEP的生物降解——土壤微观环境降解、途径及其生物修复潜力分析

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04459-8

Moumita Mondal, Sujan Gayen, Subhankar Chatterjee

{"title":"Biodegradation of phthalates DIBP, DMP, and DEP by Paenarthrobacter sp. strain PH1 - analysis of degradation, pathway, and its bioremediation potentiality in soil microcosm.","authors":"Moumita Mondal, Sujan Gayen, Subhankar Chatterjee","doi":"10.1007/s11274-025-04459-8","DOIUrl":"10.1007/s11274-025-04459-8","url":null,"abstract":"Phthalic acid esters (PAEs), a class of anthropogenic hazardous chemicals, have become one of the lead toxic chemicals on the environmental contamination list. Their weak chemical bonding makes them easily assimilated into the environment, leading to serious environmental concerns. This study assessed the degradation of three low-molecular-weight phthalates, di-isobutyl phthalate (DIBP), diethyl phthalate (DEP), and dimethyl phthalate (DMP), in batch culture and artificial soil microcosms by a gram-positive strain Paenarthrobacter sp. PH1, isolated from a municipal waste dumping site in Chennai, Tamil Nadu, India. An intracellular esterase enzyme activity was found to be involved in phthalate degradation. Quantitative biodegradation analysis, conducted using high-performance liquid chromatography, depicted that the strain PH1 could degrade 1 g/L of DIBP, DEP, and DMP within 24 h, 48 h, and 60 h, respectively, in mineral media under batch shake culture conditions (28 °C, pH 7.0, rpm 180). When a mixture of three phthalates was used as a carbon source, all PAEs were completely degraded within 72 h. Besides that, artificial soil microcosm experiments exhibited that the strain PH1 could significantly degrade 63.38% and 66.89% of DIBP, 65.79% and 69.7% of DEP, and 64.91% and 69.07% of DMP within 12 days in sterile soil and unsterile soil, respectively. This is the first report on mixed phthalates degradation by any single strain in the soil microcosm. The findings highlight the potentiality of the strain PH1 towards the bioremediation of phthalate-contaminated soil, especially under mixed phthalate conditions, making it a potential candidate for multi-phthalate degradation in the soil environment.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"229"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-cultivation strategies for natural product discovery from actinomycetes: unlocking silent secondary metabolism with mycolic acid-containing bacteria. 从放线菌中发现天然产物的共同培养策略：解锁与含霉菌酸细菌的沉默次级代谢。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04406-7

Shumpei Asamizu

{"title":"Co-cultivation strategies for natural product discovery from actinomycetes: unlocking silent secondary metabolism with mycolic acid-containing bacteria.","authors":"Shumpei Asamizu","doi":"10.1007/s11274-025-04406-7","DOIUrl":"10.1007/s11274-025-04406-7","url":null,"abstract":"Bacteria form consortia as integral components of diverse ecosystems, where they interact with various organisms. Within these communities, bacterial-bacterial communication plays a pivotal role by driving numerous specific interactions. A key aspect of this chemical communication is the production of secondary metabolites. Recent research demonstrates that interspecies interactions between microorganisms can serve as physiological triggers, activating silent biosynthetic gene clusters and leading to the synthesis of novel secondary metabolites by the interacting species. This review focuses on mixed cultivation strategies involving actinobacteria, with an emphasis on utilizing mycolic acid-containing bacteria such as Tsukamurella pulmonis as inducer organisms. It comprehensively examines recent advances striving to understand these bacterial interactions, specifically involving the ability of actinomycetes to recognize and respond to mycolic acid-containing bacteria to activate secondary metabolism. Furthermore, the genetic basis of secondary metabolism activation was explored and newly discovered secondary metabolites induced by actinobacteria-mycolic acid-containing bacteria co-culture were highlighted. Finally, the integration of combined-culture strategies with genetic engineering methods and the ecological relevance of actinobacteria-mycolic acid-containing bacteria interactions were discussed. These bacterial interactions provide an excellent model system for understanding the molecular mechanisms regulating secondary metabolism and could open new avenues for drug discovery.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"217"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of microbial community to different depths in volcanic soils of Nvshan Volcano. 女山火山土壤微生物群落对不同深度的响应

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04456-x

Jin Chen, Haining Wang, Ye Cui, Yiming Dai, Yueming Bao, Rui Qin, Qixiu Cheng, Xiaoyu Li

引用次数: 0

Production, downstream, stability, bioactivities, and applications studies of violacein and related compounds. 紫罗兰素及其相关化合物的生产、下游、稳定性、生物活性及应用研究。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04434-3

Simone Aparecida de Lima Scaramussa, Caroline Kie Ishimoto, Juliano Lemos Bicas

引用次数: 0

Comparative microbiomes reveal microbial signatures in coffee fermentation and flavor development across distinct Brazilian biomes. 比较微生物组揭示了咖啡发酵和风味发展中不同巴西生物群系的微生物特征。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04376-w

Natan Wiele, Gilberto Vinícius de Melo Pereira, Alexander da Silva Vale, Vitória de Mello Sampaio, Ana Isabel Ribeiro-Barros, Agnes de Freitas Diniz de Souza, Diogo Vinícius Nogueira Dos Santos, Aristóteles Góes-Neto, Carlos Ricardo Soccol

引用次数: 0

Control of diarrhea and intestinal damage caused by enteropathogenic Escherichia coli using infant formula fermented with Lacticaseibacillus paracasei BMK2005: in vitro and in vivo evidences. 用副干酪乳杆菌BMK2005发酵婴儿配方奶粉控制肠致病性大肠杆菌引起的腹泻和肠道损伤：体外和体内证据

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04295-w

Kamel Bendjeddou, Amel Ait Meddour, Ahmed Adjebli, Roza Ourtirane, Djamila Sadoun, Djamel Drider

{"title":"Control of diarrhea and intestinal damage caused by enteropathogenic Escherichia coli using infant formula fermented with Lacticaseibacillus paracasei BMK2005: in vitro and in vivo evidences.","authors":"Kamel Bendjeddou, Amel Ait Meddour, Ahmed Adjebli, Roza Ourtirane, Djamila Sadoun, Djamel Drider","doi":"10.1007/s11274-025-04295-w","DOIUrl":"10.1007/s11274-025-04295-w","url":null,"abstract":"Lacticaseibacillus paracasei BMK2005 (L. paracasei BMK2005) was isolated from the feces of a 1-year-old healthy Algerian infant. This strain, identified by 16S rDNA sequencing and API system, has demonstrated a remarkable antibacterial activity against enteropathogenic Escherichia coli in vitro and in vivo. In addition, this strain was shown to survive in a variety of environmental conditions that mimic the gastrointestinal tract conditions such as different pH values, bile salts and digestive enzymes. Moreover, L. paracasei BMK2005 was γ-hemolytic and sensitive to various antibiotics, supporting a safe characteristic for its application. Next, the anti-EPEC activity was then evaluated in an infant formula composed of 50% whey and 50% skim milk. A significant reduction in EPEC counts was observed after 24 h of incubation. These inhibitory properties were confirmed in an animal model using ten holoxenic rabbits made diarrheic by EPEC ingestion. L. paracasei BMK2005 was able to reduce the number of EPEC in the feces of diarrheic animals treated with the milk formula fermented by this strain. To support this study, histopathologic sections of the intestines of treated and untreated rabbits revealed clear injury in the case of animals infected with EPEC, an effect characterized by damaged villosities, while normal villosities were observed in samples from control or treated animals, delineating the protective effect of L. paracasei BMK2005.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"209"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0