World journal of microbiology & biotechnology最新文献

{"title":"Deciphering the endomicrobiome of Podophyllum hexandrum to reveal the endophytic bacterial-association of in-planta podophyllotoxin biosynthesis.","authors":"Manju Kumari, Anish Tamang, Mohit Swarnkar, Pawan Kumar, Dinesh Kumar, Ashish Rambau Warghat, Vipin Hallan, Shiv Shanker Pandey","doi":"10.1007/s11274-024-04245-y","DOIUrl":"10.1007/s11274-024-04245-y","url":null,"abstract":"<p><p>Understanding the change in plant-associated microbial diversity and secondary metabolite biosynthesis in medicinal plants due to their cultivation in non-natural habitat (NNH) is important to maintain their therapeutic importance. Here, the bacterial endomicrobiome of Podophyllum hexandrum plants of natural habitat (NH; Kardang and Triloknath locations) and NNH (Palampur location) was identified and its association with the biosynthesis of podophyllotoxin (PTOX) was revealed. Rhizomes (source of PTOX) of plants of NH had highest endophytic bacterial diversity compared to NNH-plants. Presence of plant-location and tissue-specific distinct and common taxa were also identified. Acinetobacter, Ralstonia and Pseudomonas were identified as core taxa, present in plants of both NH and NNH. Predictive functional analysis of endophytic communities revealed abundant presence of genes encoding initial enzymes of PTOX biosynthesis and plant growth promotion in the rhizomes and roots of Kardang locations. Higher accumulations of secondary metabolites such as PTOX (2.78 and 2.11 folds in Kardang and Triloknath rhizomes, respectively; 1.48 and 1.71 fold in Kardang and Triloknath roots, respectively), Picropodophyllotoxin (3.08 fold in Kardang rhizomes), Quercetin (1.65 fold in Kardang and 1.32 fold in Triloknath rhizomes; 3.07-fold in Kardang and 1.60 fold in Triloknath roots) and Kaempferol (1.66 and 1.24-fold in Kardang and Triloknath rhizomes, respectively; 2.91 and 1.94-fold in Kardang and Triloknath roots, respectively) were also found in NH compared to NNH. This study provides novel insight into the change in the endomicrobiome of NH and NNH-plants and their correlation to secondary metabolites biosynthesis, and that must be considered for cultivation practices.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"38"},"PeriodicalIF":4.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the antibacterial efficacy of bee venom against multidrug-resistant pathogenic bacteria: Escherichia coli, Salmonella typhimurium, and Enterococcus faecalis.","authors":"Emad H El-Bilawy, Islam Mamdouh, Said Behiry, Islam I Teiba","doi":"10.1007/s11274-024-04248-9","DOIUrl":"10.1007/s11274-024-04248-9","url":null,"abstract":"<p><p>Bee venom (BV) represents a promising natural alternative to conventional antibiotics, particularly significant given its broad-spectrum antimicrobial activity and potential to address the growing challenge of antimicrobial resistance. The prevalence of antimicrobial-resistant microorganisms (AMR) is a global burden that affects human health and the economies of different countries. As a result, several scientific communities around the world are searching for safe alternatives to antibiotics. In this context, the present study represents a comprehensive investigation to evaluate the antibacterial effect of bee venom (BV) against Escherichia coli ATCC8739, Salmonella typhimurium ATCC14028, and Enterococcus faecalis ATCC25923. One mg of BV was extracted using 1 mL of DMSO to obtain a 1000 µg/mL solution. The chemical profile of the BV extract was determined using GC-MS, which revealed the presence of bioactive molecules with antimicrobial properties, such as astaxanthin, hycanthone, and fucoxanthin. The BV extract was tested against bacterial strains using different concentrations to obtain the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The results obtained revealed a high antibacterial activity of BV against the three strains with the highest MIC/MBC values of 12.5/25 µgml^- 1 against S. typhimurium. The antibacterial activity of the BV extract was compared to five conventional antibiotics using the disc diffusion method. The results showed a high antibacterial activity of the BV extract compared to different antibiotics with the largest inhibition zone obtained against E. faecalis at a value of 15 ± 0.22 mm compared to 9 ± 0.13 for azithromycin. The mode of action of BV, examined using scanning electron microscopy, proved a high effect of BV on the permeability of the bacterial plasma membrane. This study demonstrates bee venom's promising potential as a natural and eco-friendly antimicrobial agent, with activity against multiple bacterial strains, suggesting it may serve as an alternative to conventional antibiotics. The findings highlight the potential applications of BV in medical, agricultural, and veterinary fields, offering a sustainable solution to combat antimicrobial resistance. However, further studies are needed to fully assess its broad-spectrum antibacterial potential.Clinical trial number Not applicable.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"40"},"PeriodicalIF":4.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metabolomic analysis of Haematococcus pluvialis during hyperaccumulation of astaxanthin under the high salinity and nitrogen deficiency conditions.","authors":"Yong Dou, Liuyang Cheng, Yiwen Wang, Guihai Yu, Wenli Zhou","doi":"10.1007/s11274-025-04254-5","DOIUrl":"10.1007/s11274-025-04254-5","url":null,"abstract":"<p><p>Revealing the differences of metabolite profiles of H. pluvialis during hyperaccumulation of astaxanthin under the high salinity and nitrogen deficiency conditions was the key issues of the present study. To investigate the optimum NaCl and NaNO₃ concentration and the corresponding metabolic characteristic related to the astaxanthin accumulation in H. pluvialis, a batch culture experiment was conducted. The results indicated that 7.5 g·L^- 1 and 0 g·L^- 1 (nitrogen deficiency) were the optimum NaCl and NaNO₃ levels for the astaxanthin accumulation respectively, under which the highest astaxanthin contents reached up to 7.51mg·L^- 1 and 5.60mg·L^- 1. A total of 132 metabolites were identified using LC-MS/MS technique, among which 30 differential metabolites with statistical significance were highlighted. Subsequently, 18 and 10 differential metabolic pathways in the high salinity (HS) and nitrogen-deficient (ND) treatments were extracted and annotated respectively. The values of Fv/Fm, Yield and NPQ were all at the highest level in the ND group during the experiment. The levels of the metabolites in the ND group were almost lower than those both in the control (CK) and HS group, while which in the HS group were substantially at the higher or close levels compared to the CK group. Finally, 7 metabolic markers related to the astaxanthin accumulation were highlighted in the HS and ND group respectively. L-Proline, L-Aspartate, Uridine 5'-monophosphate (UMP), Succinate, L-2-Hydroxygluterate, L-Valine and Inosine 5'-monophosphate (IMP) were identified as the metabolic markers in the HS group, whose fold change were 0.85, 4.14, 0.31, 0.66, 3.10, 1.32 and 0.30. Otherwise, the metabolic markers were Glyceric acid, Thymine, sn-Glycerol 3-phosphate, Glycine, Allantoic acid, L-Valine and IMP in the ND group, with the fold change 0.23, 2.11, 0.38, 0.41, 0.50 and 2.96 respectively. The results provided the comparative metabolomic view of astaxanthin accumulation in H. pluvialis under the different cultivation conditions, moreover showed a novel insights into the astaxanthin production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"37"},"PeriodicalIF":4.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The hidden language of plant-beneficial microbes: chemo-signaling dynamics in plant microenvironments.","authors":"Nikita Bisht, Tanya Singh, Mohd Mogees Ansari, Puneet Singh Chauhan","doi":"10.1007/s11274-025-04253-6","DOIUrl":"10.1007/s11274-025-04253-6","url":null,"abstract":"<p><p>Plants and microorganisms coexist within complex ecosystems, significantly influencing agricultural productivity. Depending on the interaction between the plant and microbes, this interaction can either help or harm plant health. Microbes interact with plants by secreting proteins that influence plant cells, producing bioactive compounds like antibiotics or toxins, and releasing molecules such as N-acyl homoserine lactones to coordinate their behaviour. They also produce phytohormones which help regulate growth and stress responses in plants. Plants also interact with the associated microorganisms by exuding substances such as carbon and nitrogen sources, quorum-sensing molecules, peptide signals, secondary metabolites such as flavonoids and strigolactones. A successful exchange of chemical signals is essential for maintaining these associations, with significant implications for plant growth and development. This review explores the intricate array of signaling molecules and complex mechanisms governing plant-microbe interactions, elucidating the pivotal role of chemo-communication pathways. By examining these molecular dialogues, the review aims to deepen our understanding of chemo-signaling molecules, paving the way for future applications of these networks in promoting agricultural sustainability.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"35"},"PeriodicalIF":4.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic biology meets Aspergillus: engineering strategies for next-generation organic acid production.","authors":"Yang Wu, Qian-Hui Xu, Zi-Lei Chen, Lin-Hui Yang, Dong-Sheng Guo","doi":"10.1007/s11274-024-04246-x","DOIUrl":"10.1007/s11274-024-04246-x","url":null,"abstract":"<p><p>Organic acids constitute a vital category of chemical raw materials. They have extensive applications in industries such as polymers, food, and pharmaceuticals. Currently, industrial production predominantly relies on microbial fermentation. Aspergillus, due to its unique metabolic capabilities, has become an important microbial resource for organic acid production. In recent years, there has been a growing emphasis on genetic engineering of Aspergillus to increase its yield of organic acids. This review provides a comprehensive overview of the current advancement and future directions in the application of genetic engineering techniques to enhance organic production in Aspergillus, specifically highlighting achievement in reconstructing metabolic pathways for desired products, eliminating by-products, modifying regulatory pathways, and engineering mycelial morphology. Furthermore, this review also focuses on the strategies and genetic tools applied in Aspergillus, with particular emphasis on the potential applications and challenges of CRISPR-based biosensors in organic acid fermentation. By providing insights into these developments, we aim to offer theoretical guidance and innovative approaches for enhancing the efficiency of Aspergillus strains in industrial organic acid production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"36"},"PeriodicalIF":4.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into Thermomonas hydrothermalis: potential applications in industrial biotechnology.","authors":"Songül Yaşar Yıldız","doi":"10.1007/s11274-024-04240-3","DOIUrl":"10.1007/s11274-024-04240-3","url":null,"abstract":"<p><p>Thermomonas hydrothermalis, a thermophilic bacterium isolated from hot springs, exhibits unique genomic features that underpin its adaptability to extreme environments and its potential in industrial biotechnology. In this study, we present a comparative genomic analysis of two strains, DSM 14834 and HOT.CON.106, revealing distinct metabolic pathways and stress response mechanisms. The genome annotation highlighted strain-specific variations, such as enhanced motility and chemotaxis capabilities in HOT.CON.106 and a stronger genomic stability emphasis in DSM 14834. Comparative analysis with other Thermomonas species demonstrated that T. hydrothermalis possesses a unique genomic architecture, including genes for thermostable enzymes (e.g., amylases and pullulanases) and secondary metabolite biosynthesis. These enzymes and metabolites have significant industrial potential in high-temperature processes such as bioenergy production, bioplastics synthesis, and bioremediation. The findings underscore the relative differentiation between the strains and their broader implications for sustainable biotechnology, offering a basis for further exploration of thermophilic microorganisms in industrial applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"30"},"PeriodicalIF":4.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demonstration of adapted packed-bed bioreactor for accurate and rapid estimation of biochemical oxygen demand: insights into the influence of microbial community structure and functions.","authors":"Hoang-Duy P Nguyen, Bao-Ngoc T Le, Hong-Nhung Nguyen, Thuy-Van T Nguyen, Thanh-Linh H Duong, Tien-Cuong Hoang, Nguyen Phuc Thanh Duy, Minh V Nguyen, Linh N Duong, Loan Q Le, Thuy-Phuong T Pham","doi":"10.1007/s11274-025-04249-2","DOIUrl":"10.1007/s11274-025-04249-2","url":null,"abstract":"<p><p>This study demonstrated a novel approach to accurately estimate 5-day biochemical oxygen demand (BOD₅) in textile wastewater using a microbial consortium from food processing wastewater fixed on coconut fibers. Although glucose-glutamic acid (GGA) has been widely known as the most preferred substrates for microbial respiration, its calibration surprisingly resulted in an overestimation of BOD₅ in textile wastewater due to its lower utilization rate compared to that of textile wastewater. After being adapted with a new nutrient environment composed of GGA and textile wastewater, the adapted packed-bed bioreactors (PBBRs) was capable of accurate estimation of BOD₅ in textile wastewater using GGA standard solution. Metagenomic analysis revealed the dominance of the genera Enterobacter, Acinetobacter, Chryseobacterium, and Comamonas in the adapted microbial community, which are recognized for their significant potential in azo dye degradation. The imputed metagenome showed an enhanced showed an enhanced abundance of \"Amino Acid Degradation\" and \"Carbohydrate Degradation\" functions, confirming the improved ability of adapted community to utilization of GGA in the standard solution. These findings suggest that adaptation of exogenous microbial consortium to a nutrient environment composed of GGA and target wastewater may shift the community to that dominated by strains having both utilization ability of GGA and target compounds which, in turn, enhance the accuracy of the adapted PBBRs for estimation of BOD₅ in target wastewater.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"31"},"PeriodicalIF":4.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the antivirulence potential of phenolic compounds to inhibit quorum sensing in Pseudomonas aeruginosa.","authors":"Emília Maria França Lima, Felipe Alves de Almeida, Uelinton Manoel Pinto","doi":"10.1007/s11274-025-04255-4","DOIUrl":"10.1007/s11274-025-04255-4","url":null,"abstract":"<p><p>Bacteria coordinate gene expression in a cell density-dependent manner in a communication process called quorum sensing (QS). The expression of virulence factors, biofilm formation and enzyme production are QS-regulated phenotypes that can interfere in human health. Due to this importance, there is great interest in inhibiting QS, comprising an anti-virulence strategy. This work aimed to evaluate the effect of selected phenolic compounds on the inhibition of QS-regulated phenotypes in Pseudomonas aeruginosa PAO1, using concentrations that do not interfere in bacterial growth. This is one of the main premises for studying the effect of compounds on QS. Firstly, an in-silico study with the LasR and RhlR proteins of P. aeruginosa by molecular docking of 82 phenolic compounds was performed. Then, a screening with 13 selected phenolic compounds was performed, using biosensor strains P. aeruginosa lasB-gfp and P. aeruginosa rhlA-gfp, which emit fluorescence when the QS system is activated. From this assay, eight compounds were selected and evaluated for inhibition of pyocyanin, rhamnolipids, proteases, elastase, and motility. The compounds variably inhibited the evaluated virulence factors. The greatest inhibitions were observed for swarming motility, achieving inhibition rates of up to 50% for baicalein (500 µM) and curcumin (50 µM). Notably, curcumin showed satisfactory inhibition for all phenotypes even at lower concentrations (12.5 to 50 µM) compared to the other compounds (125 to 500 µM). Four compounds - rosmarinic acid, baicalein, curcumin, and resveratrol - were finally tested against biofilm formation observed by optical microscopy. This study demonstrated that phenolic compounds exhibit strong in silico binding to P. aeruginosa LasR and RhlR proteins and variably inhibit QS-regulated phenotypes in vitro. Although no biofilm inhibition was observed, future studies combining compounds and exploring molecular mechanisms are recommended. These findings highlight the biotechnological potential of phenolic compounds for future applications in the food, clinical, and pharmaceutical fields.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"32"},"PeriodicalIF":4.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cu-tolerant Klebsiella variicola SRB-4 increased the nanoparticle (NP) stress resilience in garden peas (Pisum sativum L.) raised in soil polluted with varying doses of copper oxide (CuO)-NP.","authors":"Mohammad Danish, Mohammad Shahid, Zaryab Shafi, Mohammad Abul Farah, Khalid Mashay Al-Anazi","doi":"10.1007/s11274-024-04239-w","DOIUrl":"10.1007/s11274-024-04239-w","url":null,"abstract":"<p><p>Utilizing metal/nanoparticle (NP)- tolerant plant growth-promoting rhizobacteria (PGPR) is a sustainable and eco-friendly approach for remediation of NP-induced phytotoxicity. Here, Pisum sativum (L.) plants co-cultivated with different CuO-NP concentrations exhibited reduced growth, leaf pigments, yield attributes, and increased oxidative stress levels. Cu-tolerant (800 µM) Klebsiella variicola strain SRB-4 (Accession no. OR715781.1) recovered from metal-contaminated soils produced various PGP traits, including IAA, EPS, siderophore, HCN, ammonia, and solubilized insoluble P. The PGP substances were marginally increased with increasing CuO-NP concentrations. When applied, Cu-tolerant SRB-4 strain increased root length (18%), root biomass (15.3%), total chlorophyll (29%), carotenoids (30%), root N (21%), root P (23%), total soluble protein (20%) nodule number (32%), nodule biomass (39%) and leghaemoglobin content (18%) in 50 µM CuO-NP-exposed peas. Furthermore, proline, malondialdehyde (MDA), superoxide radical, hydrogen peroxide (H₂O₂) content, and membrane injury in K. variicola-inoculated and 50 µM CuO-NP-treated plants were maximally and significantly (p ≤ 0.05) reduced by 70.6, 26.8, 60.8, and 71.6%, respectively, over uninoculated but treated with similar NP doses. Moreover, K. variicola inoculation caused a significant (p ≤ 0.05) decline in Cu uptake in roots (71%), shoots (65.5%), and grains (76.4%) of peas grown in soil contaminated with 50 µM CuO-NP. The multivariate i.e. heat map and pearson correlation analyses between the NP-treated and PGPR inoculated parameters revealed a significant and strong positive corelation. The NP-tolerant indigenous beneficial K. variicola could be applied as an alternative to enhance the production of P. sativum cultivated in nano-polluted soil systems. Additionally, more investigation is required to ascertain the seed/soil inoculation effect of K. variicola SRB-4 on soil biological activities and different crops under various experimental setups.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"34"},"PeriodicalIF":4.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Modulation of S. aureus and P. aeruginosa biofilm: an in vitro study with new coumarin derivatives.","authors":"Tapas Das, Manash C Das, Antu Das, Sukhen Bhowmik, Padmani Sandhu, Yusuf Akhter, Surajit Bhattacharjee, Utpal Ch De","doi":"10.1007/s11274-024-04221-6","DOIUrl":"10.1007/s11274-024-04221-6","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"33"},"PeriodicalIF":4.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}