World journal of microbiology & biotechnology最新文献

Research progress on the function and regulatory pathways of amino acid permeases in fungi. 真菌中氨基酸渗透酶的功能和调控途径的研究进展。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-25 DOI: 10.1007/s11274-024-04199-1

Yuzhen Yang, Yanqiu Li, Jing Zhu

{"title":"Research progress on the function and regulatory pathways of amino acid permeases in fungi.","authors":"Yuzhen Yang, Yanqiu Li, Jing Zhu","doi":"10.1007/s11274-024-04199-1","DOIUrl":"https://doi.org/10.1007/s11274-024-04199-1","url":null,"abstract":"Nitrogen sources are pivotal for the formation of fungal mycelia and the biosynthesis of metabolites, playing a crucial role in the growth and development of fungi. Amino acids are integral to protein construction, constitute an essential nitrogen source for fungi. Fungi actively uptake amino acids from their surroundings, a process that necessitates the involvement of amino acid permeases (AAPs) located on the plasma membrane. By sensing the intracellular demand for amino acids and their extracellular availability, fungi activate or suppress relevant pathways to precisely regulate the genes encoding these transporters. This review aims to illustrate the function of fungal AAPs on uptake of amino acids and the effect of AAPs on fungal growth, development and virulence. Additionally, the complex mechanisms to regulate expression of aaps are elucidated in mainly Saccharomyces cerevisiae, including the Ssy1-Ptr3-Ssy5 (SPS) pathway, the Nitrogen Catabolite Repression (NCR) pathway, and the General Amino Acid Control (GAAC) pathway. However, the physiological roles of AAPs and their regulatory mechanisms in other species, particularly pathogenic fungi, merit further exploration. Gaining insights into these aspects could reveal how AAPs facilitate fungal adaptation and survival under diverse stress conditions, shedding light on their potential impact on fungal biology and pathogenicity.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"392"},"PeriodicalIF":4.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm and Extracellular Polymeric Substance (EPS) synergy: Revealing Staphylococcus's role in nitrate bioremediation. 生物膜与胞外聚合物质（EPS）的协同作用：揭示葡萄球菌在硝酸盐生物修复中的作用。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-25 DOI: 10.1007/s11274-024-04197-3

Riasa Zaffar, Ruqeya Nazir, Javaid Hameed, Mushtaq Ahmad Rather

{"title":"Biofilm and Extracellular Polymeric Substance (EPS) synergy: Revealing Staphylococcus's role in nitrate bioremediation.","authors":"Riasa Zaffar, Ruqeya Nazir, Javaid Hameed, Mushtaq Ahmad Rather","doi":"10.1007/s11274-024-04197-3","DOIUrl":"https://doi.org/10.1007/s11274-024-04197-3","url":null,"abstract":"Staphylococcus species, traditionally associated with pathogenicity, are gaining attention for their role in environmental bioremediation, particularly nitrate reduction, which is crucial for mitigating eutrophication. In this study, denitrifying, biofilm-forming Staphylococcus strains were isolated from Dal Lake, India. Biofilm formation was quantified using a microtiter plate assay, and extracellular polymeric substances (EPS) were measured by dry weight. Statistical analysis revealed a strong positive correlation between EPS production and nitrate removal efficiency (r = 0.96, p < 0.001), with EPS accounting for 92% of the variance in nitrate reduction (R2 = 0.92). Among the isolates, Staphylococcus epidermidis exhibited the highest nitrate reduction at 87% (SD = 2.3%), followed by S. succinus at 83% (SD = 2.1%), S. equorum at 77% (SD = 2.5%), and Staphylococcus sp. at 70% (SD = 2.8%). The consistency of these findings was confirmed by boxplot analysis, and the regression model's robustness was validated by residual plots showing minimal systematic error. This research work provides the first evidence of the nitrate-reducing capabilities of these Staphylococcus species, underscoring their potential in sustainable bioremediation strategies for aquatic environments. The significant correlation between EPS production and nitrate reduction highlights the critical role of biofilms in enhancing microbial remediation processes. The study not only advances the understanding of Staphylococcus in non-pathogenic roles but also suggests that these strains could be pivotal in bioremediation technologies, potentially influencing future environmental management practices.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"391"},"PeriodicalIF":4.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal efficacy and biofumigation potential of hydrophobic deep eutectic solvents: Postharvest treatment against Monilinia fructicola and Botrytis Cinerea. 疏水性深共晶溶剂的抗真菌功效和生物熏蒸潜力：果实莫尼林菌和灰葡萄孢菌的采后处理。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-25 DOI: 10.1007/s11274-024-04201-w

Teodora N Kukrić, Renata M Iličić, Tatjana M Jurić, Denis B Uka, Ferenc F Bagi, Simonida S Đurić, Boris M Popović

{"title":"Antifungal efficacy and biofumigation potential of hydrophobic deep eutectic solvents: Postharvest treatment against Monilinia fructicola and Botrytis Cinerea.","authors":"Teodora N Kukrić, Renata M Iličić, Tatjana M Jurić, Denis B Uka, Ferenc F Bagi, Simonida S Đurić, Boris M Popović","doi":"10.1007/s11274-024-04201-w","DOIUrl":"https://doi.org/10.1007/s11274-024-04201-w","url":null,"abstract":"Hydrophobic Deep Eutectic Solvents (HDES), as a subclass of Natural Deep Eutectic Solvents (NADES), present a green-chemistry alternative to toxic chemicals. As HDES are based on terpenoids, these solvents could potentially be effective antifungal agents against phytopathogens Monilinia fructicola and Botrytis cinerea that frequently cause diseases in sweet cherry fruit. To contribute to the disease prevention and management goals, as a part of this study, 30 different HDES were tested in the vapor phase, at identical concentrations of 25%, 50%, and 100%. In vitro experiments were conducted on Potato Dextrose Agar medium (PDA), while in planta experiments were carried out in hermetically sealed containers with inoculated sweet cherry fruits. All tested HDES demonstrated efficacy in suppressing the growth of M. fructicola colonies (66 - 100%) and B. cinerea colonies (37 - 100%). According to the Area Under the Disease Progress Curve (AUDPC), all HDES exhibited high efficacy in preventing disease occurrence in cherry fruits by the tested phytopathogens. This research provides the first insights into the antifungal potential of HDES in the vapor phase, with promising applications as biofumigants that minimize harmful impacts on the food - human - environment complex.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"393"},"PeriodicalIF":4.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of gamma irradiation/salmide®, a sodium chlorite-based oxy-halogen, on microbiological control and the shelf life of chicken breasts. 伽马辐照/Salmide®（一种基于亚氯酸钠的氧卤素）对鸡胸肉微生物控制和货架期的协同效应。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-22 DOI: 10.1007/s11274-024-04183-9

Reham M M Abdelkader, Assem Abolmaaty, Dina H Amin

{"title":"Synergistic effects of gamma irradiation/salmide®, a sodium chlorite-based oxy-halogen, on microbiological control and the shelf life of chicken breasts.","authors":"Reham M M Abdelkader, Assem Abolmaaty, Dina H Amin","doi":"10.1007/s11274-024-04183-9","DOIUrl":"10.1007/s11274-024-04183-9","url":null,"abstract":"A novel portable chamber was developed to extend the shelf life of chicken breasts through a synergistic treatment of gamma irradiation and Salmide®, a sodium chlorite-based oxy-halogen. This combination successfully enhanced the shelf life by utilizing a low dosage of gamma irradiation alongside low concentrations of Salmide (200 ppm sodium chlorite). Fresh chicken breast samples were treated with gamma irradiation, then packed in ice containing Salmide within the portable chamber, and subsequently stored for 20 days in a refrigerator at 4 °C ± 1. The study investigated aerobic bacterial counts, sensory analysis, and Thiobarbituric acid (TBA) levels. Results showed that Salmide alone significantly reduced microbial counts and extended shelf life by 8 days. Gamma irradiation at 1 kGy, either alone or combined with Salmide, caused a sequential reduction in total aerobic bacterial counts by 2,3 logarithmic cycles, respectively, extending the storage period to 12 days. Furthermore, a 16 day shelf life extension was achieved with gamma irradiation at 3 kGy, either alone or in combination with Salmide, resulting in a reduction of total aerobic bacteria by 5 logarithmic cycles. This study is the first to employ Salmide in conjunction with gamma irradiation as an innovative technology in a portable chamber to enhance the safety and shelf life of chicken breasts during storage in the designed portable chamber.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"390"},"PeriodicalIF":4.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide. 挖掘坏死葡萄球菌 H16 的潜力，将其作为利用二氧化碳生产生物产品的平台。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-22 DOI: 10.1007/s11274-024-04200-x

Yuheng Wang, Lin Cui, Lijuan Ding, Xiaoyun Su, Huiying Luo, Huoqing Huang, Yuan Wang, Bin Yao, Jie Zhang, Xiaolu Wang

{"title":"Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide.","authors":"Yuheng Wang, Lin Cui, Lijuan Ding, Xiaoyun Su, Huiying Luo, Huoqing Huang, Yuan Wang, Bin Yao, Jie Zhang, Xiaolu Wang","doi":"10.1007/s11274-024-04200-x","DOIUrl":"10.1007/s11274-024-04200-x","url":null,"abstract":"The rapid global increase in fossil fuel and energy consumption has resulted in the accumulation of greenhouse gases, especially carbon dioxide (CO2), thus contributing to climate change. Therefore, transforming CO2 into valuable products could yield beneficial outcomes. In this review, the capabilities of Cupriavidus necator H16, a light-independent chemoautotrophic bacterium, as a host platform for the transformation of CO2 into diverse products are explored. We begin by examining the progress in synthetic biology toolkits, gas fermentation technologies, and engineering approaches, considering the chemoautotrophic metabolic traits of C. necator to enhance the capacity of the strain for CO2 fixation. Additionally, recent research focused on the metabolic engineering of C. necator H16 for the conversion of CO2 into biodegradable plastics, biofuels, bioactive compounds, and single-cell proteins was reviewed. Finally, we address the limitations affecting the advancement and utilization of C. necator H16 strain, such as inefficiencies and the range of product types, and offer several recommendations for enhancement. This review acts as a resource for the development of C. necator H16 cell factories and the industrial manufacture of products derived from CO2.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"389"},"PeriodicalIF":4.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total carbohydrate consumption through co-fermentation of agro-industrial waste: use of wild-type bacterial isolates specialized in the conversion of C-5 sugars to high levels of lactic acid with concomitant metabolization of toxic compounds. 通过共同发酵农用工业废物消耗碳水化合物总量：使用专门将 C-5 糖转化为高浓度乳酸并同时代谢有毒化合物的野生型细菌分离物。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-21 DOI: 10.1007/s11274-024-04202-9

Sheila Montipó, Elisa Bellan Menegussi, Marli Camassola, Ola Wallberg, Mats Galbe

{"title":"Total carbohydrate consumption through co-fermentation of agro-industrial waste: use of wild-type bacterial isolates specialized in the conversion of C-5 sugars to high levels of lactic acid with concomitant metabolization of toxic compounds.","authors":"Sheila Montipó, Elisa Bellan Menegussi, Marli Camassola, Ola Wallberg, Mats Galbe","doi":"10.1007/s11274-024-04202-9","DOIUrl":"https://doi.org/10.1007/s11274-024-04202-9","url":null,"abstract":"Value-added bioproducts are linked to the expansion of lignocellulosic biorefineries based on agro-industrial waste and local economic growth. Thus, the aim of this study was to pretreat rice hull (RH), a highly recalcitrant biomass, with saturated steam and convert it to lactic acid (LA). Strategically, the individual fractions and the blend of detoxified liquor and water-insoluble solids were used as substrate in the simultaneous saccharification and co-fermentation (SSCF) by wild-type bacteria. The microbial consortium between Pediococcus acidilactici and Acetobacter cerevisiae enabled the metabolization of all the xylose contained in the liquor, as well as the consumption of all minor sugars when using the blend. Assays resulted in the production of 106.2 g L- 1 of LA. Furthermore, A. cerevisiae promoted complete degradation of 5-HMF/furfural in a short period of time. This study demonstrates the benefits provided by processes integration (SSCF/blend) employing high solids load (22% w/v), representing an innovative and economically interesting approach.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"388"},"PeriodicalIF":4.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uptake of lead, cadmium and copper by heavy metal-resistant Pseudomonas aeruginosa strain DR7 isolated from soil. 从土壤中分离出的抗重金属铜绿假单胞菌 DR7 菌株对铅、镉和铜的吸收。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-21 DOI: 10.1007/s11274-024-04194-6

Jianhui Zhang, Zainura Zainon Noor, Nurul Huda Baharuddin, Siti Aminah Setu, Mohd Amir Asyraf Mohd Hamzah, Zainul Akmar Zakaria

{"title":"Uptake of lead, cadmium and copper by heavy metal-resistant Pseudomonas aeruginosa strain DR7 isolated from soil.","authors":"Jianhui Zhang, Zainura Zainon Noor, Nurul Huda Baharuddin, Siti Aminah Setu, Mohd Amir Asyraf Mohd Hamzah, Zainul Akmar Zakaria","doi":"10.1007/s11274-024-04194-6","DOIUrl":"https://doi.org/10.1007/s11274-024-04194-6","url":null,"abstract":"This study highlights the biosorption capacity for Cd (II), Cu (II) and Pb (II) by a locally isolated Pseudomonas aeruginosa DR7. At initial concentrations of 150 mg L-1 and 240 min of contact time, P. aeruginosa DR7 showed a 62.56 mg/g removal capacity for Cd (II) at an optimum pH of 6.0, 72.49 mg/g for Cu (II) at an optimum pH of 6.0, and 94.2 mg/g for Pb (II) at an optimum pH of 7.0. The experimental data of Cd (II), Cu (II), and Pb (II) adsorbed by the pseudo-second-order kinetic model correlates well with P. aeruginosa DR7, with R2 all above 0.99, showing that the fitting effect was satisfactory. The isothermal adsorption processes of Cd (II) (0.980) and Cu (II) (0.986) were more consistent with the Freundlich model, whereas Pb (II) was more consistent with the Langmuir model (0.978). FTIR analysis suggested the involvement of hydroxyl, carbonyl, carboxyl, and amine groups present in the inner regions of P. aeruginosa cells during the biosorption process. SEM-EDS analysis revealed that after contact with metals, there were slight changes in the surface appearance of the cells, which confirmed the deposition of metals on the bacterial surface. There was also the possibility of the metals being translocated into the bacterial inner regions by the appearance of electron-dense particles, as observed using TEM. As a conclusion, the removal of metals from solutions using P. aeruginosa DR7 was a plausible alternative as a safe, cheap, and easily used biosorbent.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"387"},"PeriodicalIF":4.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competition-driven phenotypic plasticity in Iron acquisition and aromatic utilization confers a fitness advantage to Pseudomonas putida in an Iron-limited rhizospheric environment. 铁获取和芳香利用的竞争驱动表型可塑性使假单胞杆菌在铁有限的根瘤环境中具有适应优势。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-20 DOI: 10.1007/s11274-024-04192-8

Hiren Joshi, Atif Khan

{"title":"Competition-driven phenotypic plasticity in Iron acquisition and aromatic utilization confers a fitness advantage to Pseudomonas putida in an Iron-limited rhizospheric environment.","authors":"Hiren Joshi, Atif Khan","doi":"10.1007/s11274-024-04192-8","DOIUrl":"10.1007/s11274-024-04192-8","url":null,"abstract":"Iron scarcity poses a critical challenge for rhizospheric bacteria like Pseudomonas putida in the competitive rhizosphere. Despite its dependence on iron for essential functions such as root colonization, motility, and aromatic compound utilization, P. putida exhibits limited capability for heterologous siderophore utilization and primarily relies on the secretion of a single siderophore, pyoverdine. This study investigates the mechanisms by which P. putida acquires iron in an iron-limited, aromatic-rich, rhizosphere-like environment. Our findings demonstrate that P. putida exhibits significant phenotypic plasticity, dynamically modulating pyoverdine secretion in response to competitive pressures and substrate availability. This adaptive strategy optimizes energy expenditure and iron acquisition, providing a competitive advantage. Comparative gene expression analysis supports these observations, revealing the molecular underpinnings of this plasticity. Enhanced pyoverdine production driven by competition compensates for the bacterium's limited siderophore repertoire and facilitates rapid aromatic compound utilization, conferring a distinct fitness advantage in iron-deprived conditions. This study elucidates the complex interplay between competition, iron uptake, and aromatic compound utilization that underpins the rhizospheric success of P. putida.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"386"},"PeriodicalIF":4.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and purification of a novel calcium-independent thermostable, α-amylase produced by Bacillus licheniformis UDS-5. 地衣芽孢杆菌 UDS-5 产生的新型钙依赖性恒温 α 淀粉酶的优化和纯化。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-19 DOI: 10.1007/s11274-024-04188-4

Sadikhusain Suthar, Disha Joshi, Harsh Patel, Darshan Patel, Bhavtosh A Kikani

{"title":"Optimization and purification of a novel calcium-independent thermostable, α-amylase produced by Bacillus licheniformis UDS-5.","authors":"Sadikhusain Suthar, Disha Joshi, Harsh Patel, Darshan Patel, Bhavtosh A Kikani","doi":"10.1007/s11274-024-04188-4","DOIUrl":"10.1007/s11274-024-04188-4","url":null,"abstract":"Microbial amylases should essentially remain active at higher temperatures, and in the alkaline pH and a range of surfactants to be suitable as detergent additives. In the present study, a thermophilic amylase producing bacterium, Bacillus licheniformis UDS-5 was isolated from Unai hot water spring in Gujarat, India. It was identified as a potent amylase producer during starch plate-based screening process. Therefore, the physicochemical parameters influencing amylase production were optimized using Plackett-Burman design and Central Composite Design. The amylase was purified through ammonium sulfate precipitation, size exclusion and ion exchange chromatography, achieving the purification fold and yield to be 9.2 and 40.6%, respectively. The enzyme displayed robust stability and activity across a wide range of temperatures and pHs, with an increased half-life and reduced deactivation rate constant. The amylase exhibited optimal catalysis at 70 °C and pH 8. The kinetic studies revealed Km and Vmax values of 0.58 mg/mL and 2528 μmol/mL/min, respectively. Besides, the purified amylase displayed stability in the presence of various metal ions, surfactants, and chelators suggesting its potential for industrial applications, particularly in the detergent industry. Moreover, detergent application studies demonstrated its efficacy in enhancing washing performance. A comparative profile on washing efficiency of the studied amylase and the commercial amylase with various detergents pointed towards its possible future use as a detergent additive.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"385"},"PeriodicalIF":4.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galactose-1-phosphate uridylyltransferase GalT promotes biofilm formation and enhances UV-B resistance of Bacillus thuringiensis. 半乳糖-1-磷酸尿苷酰转移酶 GalT 可促进苏云金芽孢杆菌生物膜的形成并增强其抗紫外线-B 的能力。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-18 DOI: 10.1007/s11274-024-04195-5

Aisha Lawan Idris, Xiao Fan, Wen Li, Hankun Pei, Musa Hassan Muhammad, Xiong Guan, Tianpei Huang

{"title":"Galactose-1-phosphate uridylyltransferase GalT promotes biofilm formation and enhances UV-B resistance of Bacillus thuringiensis.","authors":"Aisha Lawan Idris, Xiao Fan, Wen Li, Hankun Pei, Musa Hassan Muhammad, Xiong Guan, Tianpei Huang","doi":"10.1007/s11274-024-04195-5","DOIUrl":"https://doi.org/10.1007/s11274-024-04195-5","url":null,"abstract":"Ultraviolet radiation (UV) is a major abiotic stress resulting in relative short duration of Bacillus thuringiensis (Bt) biopesticides in the field, which is expected to be solved by formation of Bt biofilm with higher UV resistance. Therefore, one of the important prerequisite works is to clarify the functions of biofilm-associated genes on biofilm formation and UV resistance of Bt. In this study, comparative genomics and bioinformatic analysis indicated that BTXL6_19475 gene involved in biofilm formation of Bt XL6 was likely to encode a galactose-1-phosphate uridylyltransferase (GalT, E.C. 2.7.7.12). Heterologous expression of the BTXL6_19475 gene in Escherichia coli and detection of its GalT enzyme activity in vitro proved that the gene did encode GalT. Comparing the wild type Bt strain XL6 with galT gene knockout mutant Bt XL6ΔgalT and its complementary strain Bt XL6ΔgalT::19,475, GalT promoted the biofilm formation and enhanced the UV-B resistance of Bt XL6 likely by increasing its D-ribose production and reducing its alanine aryldamidase activity. GalT did not affect the growth and the cell motility of Bt XL6. A regulation map had been proposed to elucidate how GalT promoted biofilm formation and enhanced UV-B resistance of Bt XL6 by the cross-talk between Leloir pathway, Embden-Meyerhof glycolysis pathway and pentose phosphate pathway. Our finding provides a theoretical basis for the efficient use of biofilm genes to improve the UV resistance of Bt biofilms and thus extend field duration of Bt formulations based on biofilm engineering.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"383"},"PeriodicalIF":4.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0