World journal of microbiology & biotechnology最新文献

{"title":"Integration of top-down rhizospheric selection and bottom-up consortium engineering enhances mechanistic biodegradation of crude oil hydrocarbons and soil recovery.","authors":"Sandeep Das, Silpi Sorongpong, Rajnanda Thaosen, Piyush Pandey","doi":"10.1007/s11274-026-05351-9","DOIUrl":"https://doi.org/10.1007/s11274-026-05351-9","url":null,"abstract":"<p><p>Effective remediation of crude oil-contaminated soils enriched in recalcitrant polycyclic aromatic hydrocarbons (PAHs) requires strategies that couple microbial degradative capacity with ecological compatibility. This study evaluated an ex situ rhizoremediation system integrating Crotalaria pallida with a trait-selected hydrocarbon-degrading bacterial consortium and a biosurfactant amendment, using a combination of bottom-up functional selection and top-down rhizosphere filtering. After 90 days, consortium-amended treatments achieved up to 98.3% PAH and 94.7% total petroleum hydrocarbon (TPH) removal, accompanied by a significant decrease in PAH-associated ecological risk. GC-MS profiles showed a reduction of aliphatic and aromatic hydrocarbons and the presence of oxidized transformation products, suggesting transformation of the residual hydrocarbon fraction. Hydrocarbon attenuation was accompanied by increased soil enzymatic activities and treatment-associated restructuring of the rhizosphere bacterial community. Amplicon-based bacterial community profiling revealed shifts in key bacterial taxa, while PICRUSt analysis indicated treatment-associated differences in predicted functional pathways related to xenobiotic and hydrocarbon metabolism. Together, the chemical, biochemical, and microbial responses demonstrate that integrating C. pallida with a trait-selected bacterial consortium, supplemented with biosurfactants, can stimulate hydrocarbon attenuation while supporting the biological recovery of crude oil-contaminated soil. These findings provide an integrated rhizoremediation framework linking contaminant removal to microbial community restructuring, predicted functional potential, and a reduction in PAH-associated ecological risk.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 11","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culturomics and amplicon-based insights into the endophytic microbiome of Kalmegh (Andrographis paniculata (Burm.f.) Nees) and its implications in plant growth promotion.","authors":"Samadhan Yuvraj Bagul, Rohan Sarkar, Manish Kumar Suthar, Vinay Kumar, Shreedevasena S, Virendra Singh Rana, Debabrata Sircar, Nagaraja Reddy Rama Reddy","doi":"10.1007/s11274-026-05344-8","DOIUrl":"https://doi.org/10.1007/s11274-026-05344-8","url":null,"abstract":"<p><p>Leaf and stem endophytic microorganisms play an important role in plant physiology and secondary metabolite biosynthesis of plants, particularly in medicinal plants. Kalmegh (Andrographis paniculata (Burm.f.) Nees) is an important medicinal plant with liver-protective, anti-inflammatory, ovicidal, larvicidal, and anti-urolithatic properties. The medicinal activities are due to presence of andrographolides. This study investigated the culture-independent diversity of endophytic bacteria associated with Kalmegh at nursery, vegetative, and flowering stages using a metagenomic approach. The results revealed that Sphingomonas was the dominant bacterial genus during nursery and vegetative stages, whereas Halobacterium predominated during the flowering stage. Microbial abundance was highest in the nursery stage, followed by flowering and vegetative stages. Functional analysis showed that flowering stage endophytes were enriched with specialized carbon metabolism and aromatic compound degradation pathways, potentially influencing andrographolide biosynthesis. Chemical analysis supported these findings, showing the highest andrographolide content in leaves during flowering (3.1%). Gene expression analysis further demonstrated that increased andrographolide accumulation was associated with upregulation of methyl erythritol phosphate pathway (MEP) pathway and diterpene biosynthetic genes. Bacterial endophytes isolated from flowering-stage leaves exhibited plant growth-promotion, antifungal, and seed germination-enhancing activities. In vitro assays against Fusarium wilt and Rhizoctonia disease showed that Bacillus cereus strains KL11, KL12, and KL5 inhibited pathogen growth by 60-65%. Biopriming with strains KL3 and KL5 of Bacillus cereus improved seed germination and plant growth. Pot trials with Micrococcus luteus KES1 increased herbage yield, plant height, and andrographolide content by 43%. These findings highlight the potential of leaf and stem endophytes for sustainable improvement of Kalmegh productivity and andrographolide content in the plant.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 11","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed derived fungi modulate maize responses to Cd stress.","authors":"Muhammad Awais, Naila Shah, Mirza Abid Mehmood, Waseem Tariq, Zia Ur Rehman, Haiyan Li","doi":"10.1007/s11274-026-05345-7","DOIUrl":"https://doi.org/10.1007/s11274-026-05345-7","url":null,"abstract":"<p><p>Cadmium (Cd) contamination of agricultural soils can constrain plant growth and may pose food chain risks when Cd accumulates in edible plant tissues. Seed derived fungal isolates have been investigated as potential bioinoculants for alleviating heavy metal stress, but their isolate-specific effects across levels of Cd exposure remain insufficiently characterized. AK18 and SD64 originated from a Cd contaminated site, whereas DL57 originated from a non-contaminated site, and all three isolates were evaluated under 0, 15 and 30 mg Cd kg⁻¹. Plant growth, tissue Cd accumulation, rhizosphere Cd fractionation, chlorophyll, GSH and MDA were measured. Increasing Cd exposure was associated with reduced plant growth and chlorophyll and increased Cd accumulation and MDA. Under 30 mg Cd kg⁻¹, AK18 increased root length, shoot biomass and root biomass by 70.3%, 50.0% and 45.1%, respectively, while SD64 increased root length and shoot biomass by 37.4% and 20.3%, respectively (P < 0.05). AK18 reduced shoot Cd accumulation by 42.5%, whereas SD64 reduced root Cd concentration by 25.0%. Under Cd stress, AK18 and SD64 were associated with a shift in Cd partitioning from the exchangeable fraction (F1) toward more stable fractions (F3-F5), together with higher GSH and lower MDA levels; DL57 showed more variable responses across stress levels and measured traits. Spearman correlation and redundancy analyses identified associations among plant growth, exchangeable Cd, tissue Cd accumulation, antioxidant related responses and rhizosphere Cd partitioning. Overall, the isolates showed distinct, Cd concentration dependent effects. Because fungal identity and environmental origin were not independently replicated, the observed differences cannot be attributed specifically to environmental origin.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 11","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of heat and rhamnolipids against Listeria monocytogenes: Influence of water activity and pH.","authors":"Gustavo Vasconcelos Gomes, Marcia Nitschke","doi":"10.1007/s11274-026-05352-8","DOIUrl":"10.1007/s11274-026-05352-8","url":null,"abstract":"<p><p>Foodborne diseases remain a major public health concern, with Listeria monocytogenes being one of the most significant foodborne pathogens due to its high mortality rate and persistence in food-processing environments. This study evaluated the combined effects of heat, pH, and water activity on the antimicrobial activity of rhamnolipids (RL) against L. monocytogenes within the framework of hurdle technology. Treatments were performed at 62 °C for 10 min under different pH and water activity (a_w) conditions adjusted with NaCl or sucrose. Increasing NaCl concentrations reduced the efficacy of heat treatment alone; however, its combination with RL produced a synergistic effect, achieving reductions exceeding 5 log CFU/mL in low-a_w media containing NaCl. In sucrose-containing media, the combined treatment reduced viable populations by 4-5 log CFU/mL across all pH and a_w conditions evaluated. The hurdles also showed potential as a sanitizer formulation against L. monocytogenes attached to stainless steel surfaces, while scanning electron microscopy revealed extensive structural damage to treated cells. The combination of moderate heat and rhamnolipids exhibited synergistic antimicrobial activity under a range of pH and water activity conditions, representing a prospective strategy for controlling L. monocytogenes, particularly in low-water-activity foods with high salt or sugar contents.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 11","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization, characterization, and mechanistic evaluation of iturin and surfactin like, broad spectrum antimicrobial lipopeptides from Bacillus subtilis RKEA1 isolated from Acanthus ilicifolius.","authors":"Rasiya K T, Emmanuel Simon, Abdul Bari K K, Abhini K N, Denoj Sebastian","doi":"10.1007/s11274-026-05317-x","DOIUrl":"https://doi.org/10.1007/s11274-026-05317-x","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) continues to rise globally, largely due to limited discovery of novel antimicrobial agents capable of overcoming resistance mechanisms associated with conventional antibiotics. This challenge underscores the need to explore alternative bioactive compounds. In the present study, an endophytic Bacillus subtilis strain, RKEA1, isolated from Acanthus ilicifolius collected from an area near the Kadalundi wetland mangrove forest (Kerala, India), was evaluated for its antimicrobial potential. The culture supernatant had broad-spectrum antimicrobial activity against Gram-positive bacteria such as S. aureus, S. pyogenes, C. violatium and L. monocytogenes, and Gram-negative bacteria including K. pneumoniae, E. coli, P. aeruginosa, Shigella spp., Proteus mirabilis, S. enterica and Y. enterocolitica, and also the fungi C. albicans and C. neoformans. Bioactive lipopeptides were extracted and subjected to comprehensive characterization, including determination of molecular weight, Rf value, and mass profiling. Production parameters were optimized, and physicochemical stability of the lipopeptides were examined under varying environmental conditions. Response surface methodology (RSM) based medium optimization enhanced lipopeptide production by 1.8-fold. The minimum inhibitory concentrations (MICs) of the lipopeptides were 31 µg/mL against the tested Gram-positive bacteria and 62.5 µg/mL against Gram-negative bacteria. The minimum bactericidal concentration (MBC) against Gram-positive bacteria was 62.5 µg/mL. Furthermore, mechanistic investigations provided insight into the mode of action, revealing membrane disruptive activity. Importantly, this work offers a detailed characterization of antimicrobial lipopeptides from the endophytic Bacillus subtilis RKEA1 isolated from Acanthus ilicifolius by optimizing lipopeptide production, physicochemical stability studies, antimicrobial activity and membrane-disruptive activity. Overall, the findings highlight Bacillus subtilis RKEA1 as a promising source of antimicrobial lipopeptides with potential applications in combating resistant bacterial infections and contributing to the development of novel therapeutic agents.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 11","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of antagonistic rice phyllosphere bacteria against Pyricularia oryzae and GC-MS profiling of bioactive metabolites.","authors":"Gaby Maulida Nurdin, Baharuddin Patandjengi, Tutik Kuswinanti","doi":"10.1007/s11274-026-05343-9","DOIUrl":"https://doi.org/10.1007/s11274-026-05343-9","url":null,"abstract":"<p><p>Rice phyllosphere bacteria have potential as biocontrol candidates against foliar pathogens, but their antagonistic potential against Pyricularia oryzae remains poorly characterized. This study aimed to identify and evaluate rice phyllosphere bacteria exhibiting antagonistic activity against P. oryzae, followed by ultrastructural observation and metabolite profiling of the selected isolate using scanning electron microscopy (SEM) and gas chromatography-mass spectrometry (GC-MS). A total of 75 bacterial isolates were obtained from the phyllosphere of healthy rice plants. Of these, 52 isolates were classified as non-pathogenic and non-hemolytic, with 12 isolates exhibiting antagonistic activity in the dual culture assay. Among them, OPW17, OPW19, and SR10 showed the highest radial growth inhibition, reaching 57.2%, 51.1%, and 50.0%, respectively, on day 9, and were selected for further characterization. Volatile-mediated inhibition assays also showed significant reductions in fungal colony growth. Molecular identification based on 16 S rRNA analysis identified OPW17 as Paenibacillus polymyxa, OPW19 as Bacillus cereus, and SR10 as Serratia marcescens. SEM observations revealed deformation and damage to fungal hyphae of P. oryzae after dual culture with bacterial antagonists. GC-MS profiling of OPW17 metabolite extract tentatively identified several compounds, including n-hexadecanoic acid and cis-13-octadecenoic acid. These findings support the potential of the selected rice phyllosphere bacterial isolate as biocontrol candidates against P. oryzae. Further studies are needed to characterize the volatile fraction, validate the activities of detected metabolites, and evaluate the selected isolates under greenhouse and field conditions.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Straw return is associated with improved morel cultivation performance and with changes in soil quality and microbial communities.","authors":"Xia Kang, Hao Tan, Jing Li, Kexin Meng, Yangxin Li, Xiang Wu","doi":"10.1007/s11274-026-05337-7","DOIUrl":"https://doi.org/10.1007/s11274-026-05337-7","url":null,"abstract":"<p><p>Returning crop straw to soil is a cornerstone practice for sustaining soil quality in grain cropping systems, yet its application to edible fungal cultivation has received comparatively little attention. Here we report a field study of rice straw incorporation in morel (Morchella sextelata) production, conducted across two cultivation sites, Xindu and Santai, in Sichuan, China. Five treatments differing in straw physical form were compared. The results showed that morel performance differed with straw physical form rather than with the presence of straw alone. Fruiting bodies formed only at the carbon-rich, nearly neutral Xindu site, where straw applied as 3-5 cm segments (T1) was associated with the largest increases in fresh yield (+ 72.2%) and in the proportion of commercial-grade fruiting bodies (+ 46.7%), together with a 23.3% higher soil quality index relative to the unamended control. At the alkaline, carbon-poor Santai site, mycelial colonization was extensive under T1, with M. sextelata reaching 29.3% of fungal reads, yet no ascocarps developed. Measured contrasts included lower soil organic carbon, higher pH and fewer days with surface temperature below 5 °C at Santai than at Xindu. T1 also showed the lowest microbial nutrient-limitation intensity at both sites. Morel yield correlated positively with urease activity, a proxy for microbial N-acquisition investment, and negatively with soil organic carbon and the soil carbon-to-nitrogen ratio (all P < 0.05). Mantel tests further revealed that, at Xindu site, NH₄⁺-N and NO₃^--N were significantly associated with the composition of the rare bacterial sub-community, and that urease activity was the most consistent variable showing significant (P < 0.05) associations with bacterial and fungal community composition. A partial least squares path model was consistent with an indirect association between straw return and yield, coinciding with a lower relative abundance of taxa that were negatively associated with yield (Ceratorhiza sp. and Planctomycetota) and with more favorable soil pH and Morchella colonization. These findings suggest that straw return in the 3-5 cm form may represent a low-cost strategy to improve morel cultivation on soils with near-neutral pH and adequate baseline organic carbon, and that crop residue management could act as a useful soil microbiome management strategy in edible fungal production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of macronutrients and monsoon on the AM fungal community structure within lateral root systems of Hevea brasiliensis.","authors":"Anchu Vinod, Minimol Ravindran, Pheba Joseph, Alex Raju, A R Aswathy, Namitha John, Rekha Karumamkandathil, Thomas Kadampanattu Uthup","doi":"10.1007/s11274-026-05342-w","DOIUrl":"https://doi.org/10.1007/s11274-026-05342-w","url":null,"abstract":"<p><p>Arbuscular mycorrhizal fungi (AMF) are important root symbionts that enhance nutrient acquisition, water uptake, stress tolerance, and soil ecosystem functioning in terrestrial plants. In perennial plantation systems like Hevea brasiliensis, understanding the dynamics of root-associated AMF communities under varying seasonal and nutrient conditions is essential for developing sustainable nutrient management strategies, particularly in emerging rubber growing regions. In the present study, we investigated the, composition, diversity, and seasonal dynamics of AMF communities directly associated with the roots of 12 year old H. brasiliensis plants maintained under long term nutrient withdrawal treatments. AMF specific barcoding regions were selectively amplified and sequenced from root samples collected during pre- and post-monsoon seasons. Species level analysis revealed seasonal differences in AMF community structure, with post-monsoon samples having comparatively higher abundance and species richness. Pre-monsoon communities were largely dominated by members of the Glomeraceae, whereas post-monsoon samples showed greater phylogenetic diversity, including representatives of Diversisporaceae, Acaulosporaceae, and Entrophosporaceae. Nutrient omission treatments showed variation in root AMF community composition with respect to the availability of phosphorus, nitrogen, potassium, calcium, and magnesium. The treatment specific occurrence of Acaulospora scrobiculata, Entrophospora infrequens, Acaulospora fragilissima, and Dominikia bernensis identified them as potential candidates for further ecological investigation under nutrient deficient conditions, whereas the consistent occurrence of Glomus, Rhizophagus intraradices, Dominikia disticha, and Dominikia gansuensis across multiple treatments suggests that they may constitute a core component of the symbiotic fungal community within rubber roots. Overall, the findings suggest that seasonal changes and nutrient stoichiometry may be associated with variation in root-associated AMF community structure in Hevea. The study also identified candidate AMF species for further investigation toward sustainable rubber cultivation under changing tropical conditions.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar-immobilized Burkholderia pyrrocinia P10 alleviates cadmium stress in pepper throughout the growth cycle: physiological responses and transcriptional regulatory features.","authors":"Tingting Yang, Yudie Zhao, Lingxiang Zeng, Lizhen Han","doi":"10.1007/s11274-026-05341-x","DOIUrl":"https://doi.org/10.1007/s11274-026-05341-x","url":null,"abstract":"<p><p>Cadmium (Cd) is a primary contaminant deteriorating agricultural soil quality in China. Pepper (Capsicum annuum L.) is a vegetable crop capable of accumulating high levels of Cd. This study was conducted to investigate the effects of a biochar-based microbial agent (Burkholderia pyrrocinia P10) on pepper growth and development under Cd stress conditions. The results showed that P10 had strong Cd resistance-related and plant growth-promoting properties. Morever, it effectively colonized the porous biochar structure. Under Cd stress (10-70 mg kg⁻¹), the biochar-immoblized P10 (PC) treatment consistently enhanced pepper growth and Cd tolerance throughout the growth period, as reflected by increased plant biomass, decreased malondialdehyde content. PC also significantly increased the number of fruits per plant and improved fruit nutritional quality, while markedly reducing soil available Cd and the acuumulation of Cd plants and fruits. These beneficial effects were superior to those of bacterial inoculation or biochar application alone. According to root transcriptomic profiling at the full-fruiting stage, compared with the effects of 50 mg kg^- 1 Cd²⁺ stress conditions, the PC treatment upregulated the expression of genes related to cell wall and cell membrane synthesis (e.g., CYP98A3, ALDH2C4, PER4, and FAD2); phytohormone biosynthesis and signaling (e.g., GH3.1, CisZOGT, amiE, GA3ox, KS, and KO); base excision repair, alleviation of spliceosome dysfunction, and protein homeostasis (e.g., NTH, AlkA, prp16, and Ubx); and glutathione and ascorbate metabolism (e.g., APX1, GME, and multiple GST genes). These findings elucidate the molecular basis of the superior efficacy of biochar-immobilized bacterial agent in enhancing Cd tolerance and reducing Cd accumulation in pepper.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in microbial succinic acid production: integrating genome engineering and fermentation strategies.","authors":"Chutchawan Phosriran, Sirima Suvarnakuta Jantama, Kuan-Chen Cheng, Kaemwich Jantama","doi":"10.1007/s11274-026-05339-5","DOIUrl":"10.1007/s11274-026-05339-5","url":null,"abstract":"<p><p>Succinic acid is a four-carbon dicarboxylic acid and important platform chemical with diverse applications in food and feed, pharmaceutical, textile, and bio-based polymer industries. Growing demand for sustainable and economically viable production routes has stimulated increasing interest in microbial routes for succinic acid biosynthesis as an alternative to conventional petrochemical processes. This mini-review summarizes recent advances in microbial succinic acid production, with particular emphasis on strain development, metabolic engineering, and fermentation-process optimization. Significant progress has been achieved through engineering of both native producers, Actinobacillus succinogenes, Mannheimia succiniciproducens, and Corynebacterium glutamicum and non-native production hosts, such as Escherichia coli, Klebsiella oxytoca, and several yeast species, leading to marked improvements in succinate concentration, yield, and productivity. Emerging approaches, including adaptive-laboratory evolution, systems-level omics, and CRISPR-Cas9, have enabled more precise control of carbon flux toward succinic acid biosynthesis while improving cellular tolerance to low pH and elevated succinic acid concentrations. In parallel, advances in fermentation and bioprocess strategies, particularly low-pH fermentation and enhanced CO₂ fixation, together with the valorization of renewable feedstocks such as lignocellulosic hydrolysates, offer promising opportunities to improve process feasibility and sustainability. However, their economic competitiveness remains strongly influenced by feedstock pretreatment, hydrolysis, detoxification, fermentation performance, and downstream processing requirements. Finally, current challenges and future perspectives for industrial-scale succinic acid production are discussed, highlighting the importance of integrating strain engineering with innovative bioprocess design to develop robust, economically competitive, and environmentally sustainable production platforms.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}