World journal of microbiology & biotechnology最新文献

Metabolic engineering of Bacillus licheniformis DW2 for ectoine production.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-02 DOI: 10.1007/s11274-024-04238-x

Xujie Li, Aoying Dong, Junru Yang, Jiang Zhu, Yangyang Zhan, Xin Ma, Dongbo Cai, Shouwen Chen

{"title":"Metabolic engineering of Bacillus licheniformis DW2 for ectoine production.","authors":"Xujie Li, Aoying Dong, Junru Yang, Jiang Zhu, Yangyang Zhan, Xin Ma, Dongbo Cai, Shouwen Chen","doi":"10.1007/s11274-024-04238-x","DOIUrl":"https://doi.org/10.1007/s11274-024-04238-x","url":null,"abstract":"Ectoine is a high-value protective agent with extensive applications in the fields of fine chemicals and biopharmaceuticals, and it is naturally synthesized by Halomonas in extreme environment, however, the current production level cannot meet the growing market demand. In this study, we aimed to develop an efficient and environmentally friendly ectoine production process using Bacillus licheniformis as the host organism. Through introducing ectoine synthetase gene cluster ectABC from Halomonas elongate, as well as optimizing ectABCHs expression by promoter and 5'-UTR optimization, ectoine titer was increased to 0.55 g/L. Furthermore, subsequent introduction of exogenous phosphoenolpyruvate carboxylase PPCEC and down-regulated expression of phosphoenolpyruvate carboxykinase PCK optimized the carbon flux through C4 anaplerotic pathway, and further benefited ectoine synthesis. Furthermore, the carbon flux towards aspartic acid accumulation was increased through optimization of glyoxylate and TCA cycles, accompanied with introducing lysCT311ICg and asdCg, and blocking by-products pathways, ectoine titer produced by B. licheniformis ECT12 was 2.00 g/L. Moreover, NADPH supply was enhanced by overexpression of exogenous NADH kinase Pos5Sc, and ectoine transportation was improved by introducing compatible solute transporter ProP from Escherichia coli, and the resulting B. licheniformis ECT14 was able to produce 2.60 g/L ectoine. Last but not the least, the ectoine yield of 3.29 g/L was attained in a 5-L fermenter. Taken together, this study not only established B. licheniformis as a framework for sustainable production of ectoine, but also paved the way for achieving the industrial production of ectoine and aspartic acid derivatives in the future.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"23"},"PeriodicalIF":4.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the impact of arsenic on symbiotic and free-living PGPB: plant growth promoting traits, bacterial compatibility and adhesion on soybean seed.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-30 DOI: 10.1007/s11274-024-04233-2

Romina Del Pilar Pramparo, Mariana Elisa Vezza, Ana Laura Wevar Oller, Melina Andrea Talano, Elizabeth Agostini

{"title":"Assessing the impact of arsenic on symbiotic and free-living PGPB: plant growth promoting traits, bacterial compatibility and adhesion on soybean seed.","authors":"Romina Del Pilar Pramparo, Mariana Elisa Vezza, Ana Laura Wevar Oller, Melina Andrea Talano, Elizabeth Agostini","doi":"10.1007/s11274-024-04233-2","DOIUrl":"https://doi.org/10.1007/s11274-024-04233-2","url":null,"abstract":"Arsenic (As) contamination in agricultural groundwater and soil is a significant economic and health problem worldwide. It inhibits soybean (Glycine max (L.) Merr.) nodulation and biological nitrogen fixation in symbiosis with Bradyrhizobium japonicum E109 (E109), a commonly used rhizobial strain for commercial biofertiliser formulation in Argentina. In the context of sustainable and climate-smart agriculture promoted by FAO, co-inoculating legumes with As-tolerant plant growth-promoting bacteria (PGPB) is suggested as a superior alternative to single inoculation. This study aimed to evaluate the impact of As on plant growth-promoting (PGP) traits -siderophore and indole acetic acid production, phosphate solubilisation, diazotrophic activity and hydrolytic enzymes activity- in E109 and three other PGPB strains: Pseudomonas sp. AW4 (AW4), Bacillus pumilus SF5 (SF5) and Bacillus toyonensis SFC 500-1E (Bt). In addition, bacterial compatibility and adhesion on soybean seed were evaluated. Arsenic significantly reduced PGP traits of E109 even at low concentrations, AW4's traits remained unchanged, while those of SF5 and Bt traits were affected (positively or negatively) only at the highest concentrations tested (500 µM arsenate, 250 µM arsenite). All PGPB strains were compatible with E109 under both control and As-stress conditions. Soybean seed adhesion was reduced for E109, only under As stress. Findings suggest that the effect of As on PGP traits is highly strain-dependent and influenced by As concentration and speciation. AW4, SF5, and Bt strains show promise for co-inoculation with E109 in soybean cultivation.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"20"},"PeriodicalIF":4.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological advances in plant growth-promoting rhizobacteria for sustainable agriculture.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-30 DOI: 10.1007/s11274-024-04231-4

Leandris Argentel-Martínez, Ofelda Peñuelas-Rubio, Angélica Herrera-Sepúlveda, Jorge González-Aguilera, Surya Sudheer, Linu M Salim, Sunaina Lal, Chittethu Kunjan Pradeep, Aurelio Ortiz, Estibaliz Sansinenea, Sandamali Harshani Kumari Hathurusinghe, Jae-Ho Shin, Olubukola Oluranti Babalola, Ugur Azizoglu

{"title":"Biotechnological advances in plant growth-promoting rhizobacteria for sustainable agriculture.","authors":"Leandris Argentel-Martínez, Ofelda Peñuelas-Rubio, Angélica Herrera-Sepúlveda, Jorge González-Aguilera, Surya Sudheer, Linu M Salim, Sunaina Lal, Chittethu Kunjan Pradeep, Aurelio Ortiz, Estibaliz Sansinenea, Sandamali Harshani Kumari Hathurusinghe, Jae-Ho Shin, Olubukola Oluranti Babalola, Ugur Azizoglu","doi":"10.1007/s11274-024-04231-4","DOIUrl":"https://doi.org/10.1007/s11274-024-04231-4","url":null,"abstract":"The rhizosphere, the soil zone surrounding plant roots, serves as a reservoir for numerous beneficial microorganisms that enhance plant productivity and crop yield, with substantial potential for application as biofertilizers. These microbes play critical roles in ecological processes such as nutrient recycling, organic matter decomposition, and mineralization. Plant growth-promoting rhizobacteria (PGPR) represent a promising tool for sustainable agriculture, enabling green management of crop health and growth, being eco-friendly alternatives to replace chemical fertilizers and pesticides. In this sense, biotechnological advancements respecting genomics and gene editing have been crucial to develop microbiome engineering which is pivotal in developing microbial consortia to improve crop production. Genome mining, which involves comprehensive analysis of the entire genome sequence data of PGPR, is crucial for identifying genes encoding valuable bacterial enzymes and metabolites. The CRISPR-Cas system, a cutting-edge genome-editing technology, has shown significant promise in beneficial microbial species. Advances in genetic engineering, particularly CRISPR-Cas, have markedly enhanced grain output, plant biomass, resistance to pests, and the sensory and nutritional quality of crops. There has been a great advance about the use of PGPR in important crops; however, there is a need to go further studying synthetic microbial communities, microbiome engineering, and gene editing approaches in field trials. This review focuses on future research directions involving several factors and topics around the use of PGPR putting special emphasis on biotechnological advances.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"21"},"PeriodicalIF":4.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The conserved protein DopA is required for growth, drug tolerance and virulence in Aspergillus fumigatus.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-30 DOI: 10.1007/s11274-024-04234-1

Qian Wei, Cong He, Xinyuan Peng, Bingyi An, Min Peng, Xiaoyu Wang, Chen Zhang, Ling Lu, Hong Sang, Qingtao Kong

{"title":"The conserved protein DopA is required for growth, drug tolerance and virulence in Aspergillus fumigatus.","authors":"Qian Wei, Cong He, Xinyuan Peng, Bingyi An, Min Peng, Xiaoyu Wang, Chen Zhang, Ling Lu, Hong Sang, Qingtao Kong","doi":"10.1007/s11274-024-04234-1","DOIUrl":"https://doi.org/10.1007/s11274-024-04234-1","url":null,"abstract":"The majority of Aspergillus fumigatus reproduction occurs asexually, with large numbers of conidiophores producing small hydrophobic conidia dispersed aerially. When healthy hosts inhale conidia, the mucosal cilia and phagocytosis by the innate immune system can remove them. However, in immunocompromised hosts, the conidia are not removed, which allows them to germinate, forming mycelium that invades host tissues and causes disease. Previously we isolated a white A. fumigatus A1j strain incapable of producing conidia and screened several genes (including dopA) with significant expression differences and mutant loci in A1j. DopA homologous proteins in other species have been partially studied and are known to participate in various membrane transport-related cellular functions. Defects in these proteins in Saccharomyces cerevisiae, Caenorhabditis elegans, and Aspergillus nidulans result in defective cell morphology and abnormal growth. In this study, we observed reduced conidia production and abnormal development of spore-producing structures in the A. fumigatus dopA null strain, compared to parental strain, and demonstrated that dopA also modulates stress response and virulence of A. fumigatus.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"19"},"PeriodicalIF":4.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multilocus sequencing analysis of the rhizobial symbionts isolated from Acacia salicina (Lindl.) grown in different regions in Tunisia reveals putative novel Bradyrhizobium species.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-30 DOI: 10.1007/s11274-024-04236-z

Mokhtar Rejili, Besma Bouznif, Mohamed Ali Benabderrahim, Mohamed Mars

{"title":"Multilocus sequencing analysis of the rhizobial symbionts isolated from Acacia salicina (Lindl.) grown in different regions in Tunisia reveals putative novel Bradyrhizobium species.","authors":"Mokhtar Rejili, Besma Bouznif, Mohamed Ali Benabderrahim, Mohamed Mars","doi":"10.1007/s11274-024-04236-z","DOIUrl":"https://doi.org/10.1007/s11274-024-04236-z","url":null,"abstract":"In this study, we investigated various chromosomal and symbiotic markers in 40 bacterial strains that nodulate an invasive alien plant, Acacia salicina Lindl. in Tunisia. Our findings showed that the native rhizobia associated to A. salicina are grouped into eight distinct RAPD electrophoretic types (RETs) (genotypes). Sequence analyses of rrs gene and three housekeeping genes (recA, rpoB and glnII) assigned sixteen isolates to three putative new lineages within the genus Bradyrhizobium. Seven strains clustered with B. rifense CTAW71T with a 91% bootstrap support, five strains grouped with B. niftali CNPSo3448T with a very low bootstrap support (60%), and four strains formed a group phylogenetically related with B. shewense ERR11T and B. centrosematis A9T. Based on nodC phylogeny and cross inoculation tests, the 16 strains are clustered within symbiovar retamae (six strains) and cyanophyllae (ten strains). Moreover, we showed by the first time in this work that the type strains B. diversitatis CNPSo4019T and B. xenonodulans 14ABT, which nodulated soybean and A. dealbata respectively, belong to the symbiovar cyanophyllae according to the results of the nodC gene analysis.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"22"},"PeriodicalIF":4.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trans-2-hexenal reduces postharvest mango stem-end rot by oxidative damage to Neofusicoccum parvum cell membranes.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-26 DOI: 10.1007/s11274-024-04235-0

Xiaoli Tan, Xiaobing Jiang, Xiumei Chen, Okwong Oketch Reymick, Chen Zhu, Nengguo Tao

{"title":"Trans-2-hexenal reduces postharvest mango stem-end rot by oxidative damage to Neofusicoccum parvum cell membranes.","authors":"Xiaoli Tan, Xiaobing Jiang, Xiumei Chen, Okwong Oketch Reymick, Chen Zhu, Nengguo Tao","doi":"10.1007/s11274-024-04235-0","DOIUrl":"https://doi.org/10.1007/s11274-024-04235-0","url":null,"abstract":"Neofusicoccum parvum is one of the most hazardous pathogens causing mango fruit decay. The present study utilized trans-2-hexenal (TH), a typical antifungal component of plant essential oils (EOs), to control N. parvum both in vivo and in vitro, and attempted to explore the mechanisms involved. The findings showed that at concentrations greater than 0.4 µL/mL, TH exhibited exceptional antifungal activity against N. parvum in vitro. TH application led to the disruption of the structural integrity of both cell walls and cell membranes, with a particular impact on the latter, as evidenced by the dramatically increased propidium iodide level, as well as reduced total lipids and ergosterol content. Further DCFH-DA staining experiments showed that TH induced mycelial reactive oxygen species (ROS) accumulation, which exacerbated cell membrane lipid peroxidation. For easier application of TH, we fabricated aerogel-loaded TH (ALTH) materials, which demonstrated excellent antifungal activity in vitro. Infestation studies on fruits demonstrated that ALTH mitigated mango stem-end rot in a dose-dependent fashion, with a concentration of 40 µL/L showing efficacy comparable to the conventional fungicide prochloraz, while maintaining fruit quality. In light of these results, TH works by inducing ROS buildup and oxidative damage to the cell membrane of N. parvum, and is a particularly promising preservative for preventing postharvest infections in mangoes.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"17"},"PeriodicalIF":4.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of bacterial quorum sensing in plant growth promotion.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-26 DOI: 10.1007/s11274-024-04232-3

Aparna Anil Singh, Anil Kumar Singh

{"title":"Role of bacterial quorum sensing in plant growth promotion.","authors":"Aparna Anil Singh, Anil Kumar Singh","doi":"10.1007/s11274-024-04232-3","DOIUrl":"https://doi.org/10.1007/s11274-024-04232-3","url":null,"abstract":"Quorum sensing (QS) also known as bacterial cell-cell communication or bacterial crosstalk is a phenomenon regulating various bacterial traits that can affect plant growth and defence. Similarities in the structure of root exudates and bacterial signalling molecules have tremendous implications governing the plant heath. The rhizosphere ecosystem being an excellent example of plant-microbe and microbe-microbe interactions harbours a variety of microorganisms exhibiting quorum sensing. Phytochemicals present in plant root exudates and QS signal molecules as well as volatile organic compounds (VOCs) produced by microorganisms work in coordination to establish intra- and inter-species communications. Interestingly, a number of plant growth promoting rhziobacterial (PGPR) activities like effective/enhanced root colonization, nutrient uptake, nodulation, nitrogen fixation, production of plant hormones, antimicrobial compounds and induction of plant defences can be attributed directly or indirectly to their quorum sensing and quenching abilities. Although not completely understood, root development, stress tolerance and defence against phytopathogens are some of the implications of such abilities which might prove beneficial for sustainable agriculture. Deciphering the mechanism of these interactions would be instrumental in improving crop health. Plant beneficial microorganisms employing QS and QS inhibition (QSI) strategies have been discussed in this review.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"18"},"PeriodicalIF":4.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, expression, and characterization of a heparinase III coupled with heparinase I for enzymatic depolymerization of heparin.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-23 DOI: 10.1007/s11274-024-04225-2

Yang-Nan Li, Chen-Yuan Zhu, Chen-Lu Xu, Shen Yu, Tong Huan, Ye-Wang Zhang

{"title":"Cloning, expression, and characterization of a heparinase III coupled with heparinase I for enzymatic depolymerization of heparin.","authors":"Yang-Nan Li, Chen-Yuan Zhu, Chen-Lu Xu, Shen Yu, Tong Huan, Ye-Wang Zhang","doi":"10.1007/s11274-024-04225-2","DOIUrl":"https://doi.org/10.1007/s11274-024-04225-2","url":null,"abstract":"A heparinase III (NsHep-III) from Niabella sp. was identified, cloned, and expressed as soluble form in E. coli BL21 (DE3). With heparin as substrate, the maximum activity of NsHep-III of 90.0 U·mg- 1 was achieved at pH 7.1 Tris-HCl (containing 15 mM Mg2+) and 30oC. The half-life of NsHep-III was determined to be 5 h at 30oC. The interactions between NsHep-III and substrates were studied by molecular docking. The combination of NsHep-III and a heparinase I from Bacteroides eggthii (BeHep-I) was employed to cleave heparin. Analysis of the enzymatic products of NsHep-III by SAX-HPLC showed seven different modified disaccharides, indicating that NsHep-III has a wide range of substrate specificity. The results of GPC analysis demonstrated that the average molecular weight of the product of heparin cleavage by the combination of NsHep-III/BeHep-I was reduced to 3969 Da, which accounted for 90% of all the components, and complied with the requirements of the European Pharmacopoeia. NsHep-III has notable activity and efficiency in cleaving heparin, which is potentially useful for the industrial production of low molecular weight heparin.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"15"},"PeriodicalIF":4.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agro-industrial wastes and their application perspectives in metal decontamination using biocomposites and bacterial biomass: a review.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-23 DOI: 10.1007/s11274-024-04227-0

Jonathan Parades-Aguilar, Sarai Agustin-Salazar, Pierfrancesco Cerruti, Veronica Ambrogi, Kadiya Calderon, Nohemi Gamez-Meza, Luis Angel Medina-Juarez

{"title":"Agro-industrial wastes and their application perspectives in metal decontamination using biocomposites and bacterial biomass: a review.","authors":"Jonathan Parades-Aguilar, Sarai Agustin-Salazar, Pierfrancesco Cerruti, Veronica Ambrogi, Kadiya Calderon, Nohemi Gamez-Meza, Luis Angel Medina-Juarez","doi":"10.1007/s11274-024-04227-0","DOIUrl":"10.1007/s11274-024-04227-0","url":null,"abstract":"Contamination of water bodies is a significant global issue that results from the deliberate release of pollutants into the environment, especially from mining and metal processing industries. The main pollutants generated by these industries are metallic wastes, particularly metals, which can cause adverse effects on the environment and human health. Therefore, it is crucial to develop effective and sustainable approaches to prevent their discharge into the environment. Biofiltration is a technique used to remediate contaminated fluids using biological processes. Microorganisms and agro-industrial wastes have been used successfully as biosorbents. Hence, this review emphasizes the innovative use of agro-industrial waste reinforced with microbial biomass as bioadsorbents, highlighting their dual capacity for metal removal through various bioremediation mechanisms. The mechanisms at play in these biocomposite materials, which offer enhanced sustainability, are also analyzed. This study contributes to the advancement of knowledge by suggesting new strategies for integrating reinforced materials in biosorption processes, thus providing a novel perspective on the potential of lignocellulosic-based systems to improve decontamination efforts. On the other hand, it shows some studies where the optimization and scaling-up of biosorption processes are reported. Additionally, the implementation of multisystem approaches, leveraging multiple bioremediation techniques simultaneously, can further enhance the efficiency and sustainability of metal removal in contaminated environments.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"16"},"PeriodicalIF":4.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation with probiotics co-cultivation improves the reproductive performance in a sow-piglet model by mother-infant microbiota transmission and placental mTOR signaling. 通过母婴微生物群传播和胎盘 mTOR 信号转导，补充益生菌联合培养可提高母猪-仔猪模型的繁殖性能。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-20 DOI: 10.1007/s11274-024-04222-5

Suchen Li, Tingting Lu, Zhixin Lin, Yuanyuan Zhang, Xinchen Zhou, Meng Li, Hui Miao, Zhiren Yang, Xinyan Han

{"title":"Supplementation with probiotics co-cultivation improves the reproductive performance in a sow-piglet model by mother-infant microbiota transmission and placental mTOR signaling.","authors":"Suchen Li, Tingting Lu, Zhixin Lin, Yuanyuan Zhang, Xinchen Zhou, Meng Li, Hui Miao, Zhiren Yang, Xinyan Han","doi":"10.1007/s11274-024-04222-5","DOIUrl":"https://doi.org/10.1007/s11274-024-04222-5","url":null,"abstract":"Maternal nutritional supplementation has a profound effect on the growth and development of offspring. FAM® is produced by co-cultivation of Lactobacillus acidophilus and Bacillus subtilis and has been demonstrated to potentially alleviate diarrhea, improve growth performance and the intestinal barrier integrity of weaned piglets. This study aimed to explore how maternal FAM improves the reproductive performance through mother-infant microbiota, colostrum and placenta. A total of 40 pregnant sows (Landrace × Large White) on d 85 of gestation with a similar parity were randomly divided into two groups (n = 20): the control group (Con, basal diet) and the FAM group (FAM, basal diet supplemented with 0.2% FAM). The experimental period was from d 85 of gestation to d 21 of lactation. The results revealed that maternal supplementation with FAM significantly decreased the number of weak-born litters and the incidence of diarrhea, as well as increasing birth weight and average weaning weight, accompanied by increased levels of colostrum nutrient composition and immunoglobulins. In addition, FAM modulated the structure of mother-infant microbiota and promoted the vertical transmission of beneficial bacteria, such as Verrucomicrobiota and Akkermansia. Furthermore, FAM contributed to improving the expression of GLU and AA transporters in the placenta, and increasing the activity of the mTOR signaling pathway. Collectively, maternal supplementation with FAM during late pregnancy and lactation could improve reproductive performance through the transmission of beneficial mother-infant microbiota and placental mTOR signaling pathway and promote fetal development.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"13"},"PeriodicalIF":4.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0