World journal of microbiology & biotechnology最新文献

{"title":"Investigation of γ-polyglutamic acid production via asynchronous saccharification and fermentation of raw corn starch.","authors":"Yutao Gou, Chenxin Niu, Fanglan Ge, Wei Li, Guiying Cheng, Shuai Jing, Hong Yang, Jiao Li, Yao Ren","doi":"10.1007/s11274-024-04141-5","DOIUrl":"https://doi.org/10.1007/s11274-024-04141-5","url":null,"abstract":"<p><p>Starch, a crucial raw material, has been extensively investigated for biotechnological applications. However, its application in γ-polyglutamic acid (γ-PGA) production remains unexplored. Based on γ-PGA output of Bacillus subtilis SCP010-1, a novel asynchronous saccharification and fermentation process for γ-PGA synthesis was implemented. The results revealed that a starch concentration of 20%, α-amylase dosage of 75 U/g, liquefaction temperature of 72℃, and γ-PGA yield of 36.31 g/L was achieved. At a glucoamylase dosage of 100 U/g, saccharification 38 h at 60℃, the yield of γ-PGA increased to 48.88 g/L. The contents of total sugar, glucose, maltose and oligosaccharide in saccharified liquid were determined. Through batch fermentation of saccharified liquid in fermentor, the γ-PGA output was elevated to 116.08 g/L. This study can offer a potential cost reduction of 40%, which can be a promising advancement in industrial γ-PGA production. Moreover, our approach can be applied in other starch-based fermentation industries.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocomposites: silver nanoparticles and bacteriocins obtained from lactic acid bacteria against multidrug-resistant Escherichia coli and Staphylococcus aureus.","authors":"Mayra Eleonora Beltrán Pineda, Daniela Fernanda Valencia Sánchez, Paula Andrea Caicedo Caycedo, José Castellanos -Rozo","doi":"10.1007/s11274-024-04151-3","DOIUrl":"https://doi.org/10.1007/s11274-024-04151-3","url":null,"abstract":"<p><p>Drug-resistant bacteria such as Escherichia coli and Staphylococcus aureus represent a global health problem that requires priority attention. Due to the current situation, there is an urgent need to develop new, more effective and safe antimicrobial agents. Biotechnological approaches can provide a possible alternative control through the production of new generation antimicrobial agents, such as silver nanoparticles (AgNPs) and bacteriocins. AgNPs stand out for their antimicrobial potential by employing several mechanisms of action that can act simultaneously on the target cell such as the production of reactive oxygen species and cell wall rupture. On the other hand, bacteriocins are natural peptides synthesized ribosomally that have antimicrobial activity and are produced, among others, by lactic acid bacteria (LAB), whose main mechanism of action is to produce pores at the level of the cell membrane of bacterial cells. However, these agents have disadvantages. Nanoparticles also have limitations such as the tendency to form aggregates, which decreases their antibacterial activity and possible cytotoxic effects, and bacteriocins have a narrow spectrum of action, require high doses to be effective, and can be degraded by proteases. Given these limitations, nanoconjugates of these two agents have been developed that can act synergistically in the control of pathogenic bacteria resistant to antibiotics. This review focuses on knowing relevant aspects of the antibiotic resistance of E. coli and S. aureus, the characteristics of these new generation antibacterial agents, and their effect alone or forming nanoconjugates that are more effective against the multiresistant mentioned bacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Employing Bacillus and Pseudomonas for phytonematode management in agricultural crops.","authors":"Rupali Gupta, Gautam Anand, Rakesh Pandey, Maya Bar, Dinesh Yadav","doi":"10.1007/s11274-024-04137-1","DOIUrl":"https://doi.org/10.1007/s11274-024-04137-1","url":null,"abstract":"<p><p>Phytonematodes are responsible for causing significant harm and reducing yields in various agricultural crops. To minimize losses caused by phytonematodes and meet the high demand for agricultural production, it is important to develop effective strategies with minimal environmental impact to manage this biotic stress. Due to the adverse environmental effects associated with synthetic pesticides, it is imperative to use beneficial bacteria, such as Bacillus and Pseudomonas spp., for biocontrol purposes to control phytonematode infestation in agricultural settings. This approach has gained considerable attraction, as there is a promising market for eco-friendly biopesticides based on bacteria that can effectively manage phytonematodes. Furthermore, biocontrol strains of Bacillus and Pseudomonas have the potential to enhance crop productivity by producing various substances that promote plant growth and development. This review aims to explore the role of Bacillus and Pseudomonas spp. in phytonematode management, elucidate different mechanisms by which these bacteria suppress nematode populations, and discuss the future prospects of utilizing these bacteria in agriculture.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genus-targeted markers for the taxonomic identification and monitoring of coagulase-positive and coagulase-negative Staphylococcus species.","authors":"S Jiménez-Velásquez, M E Pacheco-Montealegre, L Torres-Higuera, L Uribe-Gutiérrez, D Burbano-David, L L Dávila-Mora, C Renjifo-Ibáñez, A Caro-Quintero","doi":"10.1007/s11274-024-04121-9","DOIUrl":"10.1007/s11274-024-04121-9","url":null,"abstract":"<p><p>The Staphylococcus genus comprises multiple pathogenic and opportunistic species that represent a risk to public health. Epidemiological studies require accurate taxonomic classification of isolates with enough resolution to distinguish clonal complexes. Unfortunately, 16 S rRNA molecular analysis and phenotypic characterization cannot distinguish all species and do not offer enough resolution to assess intraspecific diversity. Other approaches, such as Multilocus Sequence Tagging, provide higher resolution; however, they have been developed for Staphylococcus aureus and a few other species. Here, we developed a set of genus-targeted primers using five orthologous genes (pta, tuf, tpi, groEs, and sarA) to identify all Staphylococcus species within the genus. The primers were initially evaluated using 20 strains from the Collection of Microorganisms of Interest in Animal Health from AGROSAVIA (CMISA), and their amplified sequences were compared to a set of 33 Staphylococcus species. This allowed the taxonomic identification of the strains even on close species and the establishment of intraspecies diversity. To enhance the scope and cost-effectiveness of the proposed strategy, we customized the primer sets for an Illumina paired-end amplicon protocol, enabling gene multiplexing. We assessed five genes across 177 strains, generating 880 paired-end libraries from the CMISA. This approach significantly reduced sequencing costs, as all libraries can be efficiently sequenced in a single MiSeq run at a fraction (one-fourth or less) of the cost associated with Sanger sequencing. In summary, this method can be used for precise identification and diversity analysis of Staphylococcus species, offering an advancement over traditional techniques in both resolution and cost-effectiveness.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremediation petroleum wastewater and oil-polluted soils by the non-toxigenic indigenous fungi.","authors":"Fuad Ameen, Mohammad J Alsarraf, Steven L Stephenson","doi":"10.1007/s11274-024-04146-0","DOIUrl":"https://doi.org/10.1007/s11274-024-04146-0","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Soil and wastewater samples contaminated by petroleum-related industries were collected from various locations in Saudi Arabia, a country known for its vast oil reserves. The samples were analyzed for their physicochemical properties, including the presence of metals, petroleum hydrocarbons, and aromatic compounds. A total of 264 fungal isolates were analyzed and categorized into eight groups of Aspergillus (194 isolates) and four groups of Penicillium (70 isolates). The potential of these fungal groups to grow in oil or its derivatives was investigated. Two isolates, Aspergillus tubingensis FA-KSU5 and A. niger FU-KSU69, were utilized in two remediation experiments-one targeting wastewater and the other focusing on polluted soil. The FA-KSU5 strain demonstrated complete removal of Fe&lt;sup&gt;3+&lt;/sup&gt;, As&lt;sup&gt;3+&lt;/sup&gt;, Cr&lt;sup&gt;6+&lt;/sup&gt;, Zn&lt;sup&gt;2+&lt;/sup&gt;, Mn&lt;sup&gt;2+&lt;/sup&gt;, Cu&lt;sup&gt;2+&lt;/sup&gt; and Cd&lt;sup&gt;2+&lt;/sup&gt;, with bioremediation efficiency for petroleum hydrocarbons in the wastewater from these sites ranging between 90.80 and 98.58%. Additionally, the FU-KSU69 strain achieved up to 100% reduction of Co&lt;sup&gt;2+&lt;/sup&gt;, Ba&lt;sup&gt;2+&lt;/sup&gt;, B&lt;sup&gt;3+&lt;/sup&gt;, V&lt;sup&gt;+&lt;/sup&gt;, Ni&lt;sup&gt;2+&lt;/sup&gt;, Pb&lt;sup&gt;2+&lt;/sup&gt; and Hg&lt;sup&gt;2+&lt;/sup&gt;, with removal efficiency ranging from 93.17 to 96.02% for aromatic hydrocarbons after 180 min of wastewater treatment. After 21 days of soil incubation with Aspergillus tubingensis FA-KSU5, there was a 93.15% to 98.48% reduction in total petroleum hydrocarbons (TPHs) and an 88.11% to 97.31% decrease in polycyclic aromatic hydrocarbons (PAHs). This strain exhibited the highest removal rates for Cd&lt;sup&gt;2+&lt;/sup&gt; and As&lt;sup&gt;3+&lt;/sup&gt; followed by Fe&lt;sup&gt;3+&lt;/sup&gt;, Zn&lt;sup&gt;2+&lt;/sup&gt;, Cr&lt;sup&gt;6+&lt;/sup&gt;, Se&lt;sup&gt;4+&lt;/sup&gt; and Cu&lt;sup&gt;2+&lt;/sup&gt;. Aspergillus niger FU-KSU69 achieved a 90.37% to 94.90% reduction in TPHs and a 95.13% to 98.15% decrease in PAHs, with significant removal of Ni&lt;sup&gt;2+&lt;/sup&gt;, Pb&lt;sup&gt;2+&lt;/sup&gt; and Hg&lt;sup&gt;2+&lt;/sup&gt;, followed by Co&lt;sup&gt;2+&lt;/sup&gt;, V&lt;sup&gt;+&lt;/sup&gt;, Ba&lt;sup&gt;2+&lt;/sup&gt; and B&lt;sup&gt;3+&lt;/sup&gt;. The enzymatic activity in the treated soils increased by 1.54- to 3.57-fold compared to the polluted soil. Although the mixture of wastewater and polluted soil exhibited high cytotoxicity against normal human cell lines, following mycoremediation, all treated soils and effluents with the dead fungal biomass showed no toxicity against normal human cell lines at concentrations up to 500 µL/mL, with IC&lt;sub&gt;50&lt;/sub&gt; values ≥ 1000 µL/mL. SEM and IR analysis revealed morphological and biochemical alterations in the biomass of A. tubingensis FA-KSU5 and A. niger FA-KSU69 when exposed to petroleum effluents. This study successfully introduces non-toxigenic and environmentally friendly fungal strains play a crucial role in the bioremediation of contaminated environments. Both strains serve as low-cost and effective adsorbents for bio-remediating petroleum wastewater and oil-contaminated soil. Heavy metals and hydrocarbons, the primary pollutants, were e","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of oleaginous microbes for lipid accumulation and renewable energy generation.","authors":"Yulu Yang, Mohammed Jalalah, Saeed A Alsareii, Farid A Harraz, Nandini Thakur, Yuanzhang Zheng, Adel I Alalawy, Mostafa Koutb, El-Sayed Salama","doi":"10.1007/s11274-024-04145-1","DOIUrl":"https://doi.org/10.1007/s11274-024-04145-1","url":null,"abstract":"<p><p>Biocomponents (such as lipids) accumulate in oleaginous microorganisms and could be used for renewable energy production. Oleaginous microbes are characterized by their ability to accumulate high levels of lipids, which can be converted into biodiesel. The oleaginous microbes (including microalgae, bacteria, yeast, and fungi) can utilize diverse substrates. Thus, in this study, commercially viable oleaginous microorganisms are comparatively summarized for their growth conditions, substrate utilization, and applications in biotechnological processes. Lipid content is species-dependent, as are culture conditions (such as temperature, pH, nutrients, and culture time) and substrates. Lipid production can be increased by selecting suitable microorganisms and substrates, optimizing environmental conditions, and using genetic engineering techniques. In addition, the emphasis on downstream processes (including harvesting, cell disruption, lipid extraction, and transesterification) highlights their critical role in enhancing cost-effectiveness. Oleaginous microorganisms are potential candidates for lipid biosynthesis and could play a key role in meeting the energy needs of the world in the future.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA interference: a promising biotechnological approach to combat plant pathogens, mechanism and future prospects.","authors":"Amjad Ali, Muhammad Shahbaz, Fatih Ölmez, Noor Fatima, Ummad Ud Din Umar, Md Arshad Ali, Muhammad Akram, Jaya Seelan Sathiya Seelan, Faheem Shehzad Baloch","doi":"10.1007/s11274-024-04143-3","DOIUrl":"https://doi.org/10.1007/s11274-024-04143-3","url":null,"abstract":"<p><p>Plant pathogens and other biological pests represent significant obstacles to crop Protection worldwide. Even though there are many effective conventional methods for controlling plant diseases, new methods that are also effective, environmentally safe, and cost-effective are required. While plant breeding has traditionally been used to manipulate the plant genome to develop resistant cultivars for controlling plant diseases, the emergence of genetic engineering has introduced a completely new approach to render plants resistant to bacteria, nematodes, fungi, and viruses. The RNA interference (RNAi) approach has recently emerged as a potentially useful tool for mitigating the inherent risks associated with the development of conventional transgenics. These risks include the use of specific transgenes, gene control sequences, or marker genes. Utilizing RNAi to silence certain genes is a promising solution to this dilemma as disease-resistant transgenic plants can be generated within a legislative structure. Recent investigations have shown that using target double stranded RNAs via an effective vector system can produce significant silencing effects. Both dsRNA-containing crop sprays and transgenic plants carrying RNAi vectors have proven effective in controlling plant diseases that threaten commercially significant crop species. This article discusses the methods and applications of the most recent RNAi technology for reducing plant diseases to ensure sustainable agricultural yields.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing variability among culturable phylloplane basidiomycetous yeasts from Italian agroecosystems.","authors":"Matteo Ferluga, Michele Avesani, Marilinda Lorenzini, Giacomo Zapparoli","doi":"10.1007/s11274-024-04147-z","DOIUrl":"10.1007/s11274-024-04147-z","url":null,"abstract":"<p><p>This study analysed basidiomycetous yeasts isolated from the phylloplane of crops and spontaneous plants in Italian agroecosystems. A total of 25 species belonging to 17 genera were recognized by analysing 83 isolates from vineyards and orchards, that are not treated with synthetic fungicides, and adjacent natural areas. Rhodotorula graminis and Filobasidium magnum were the most frequent species but 13 others were represented by a single isolate (e.g., Buckleyzyma salicina, Pseudozyma prolifica, and Moniliella megachiliensis). Preliminary analysis of (GTG)₅-PCR fingerprinting revealed high genetic intraspecific heterogeneity. All isolates were characterized by their production of extracellular hydrolytic enzymes and their sensitivity to six commercial fungicides used in Italy. The isolates displayed great variability in these phenotypic traits, which play an important role in the survival of yeast populations in agroecosystems. Most of them exhibited lipolytic, proteolytic, β-glucosidase and pectinolytic activities, but only three (F. magnum, Kwoniella mangroviensis and Ps. prolifica) also had cellulolytic and amylolytic activity. Most isolates were sensitive to four fungicides, and one R. graminis isolate was resistant to all six. This heterogeneity was not related to the geographical origin of the isolates. The lack of selective factors (i.e. pesticide treatments) in the sampling fields and the presence of adjacent natural areas may have favored the maintenance of an elevated level of strain diversity. This study provides new information on phylloplane basidiomycetous yeasts in agroecosystems and opens the way to further investigations into the impact of agricultural practices on the microbial diversity of these natural habitats.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11446951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and biochemical characterization of a novel halolysin from Halorubellus sp. PRR65 with a relatively high temperature activity.","authors":"Yuling Hao, Yu Jin, Aodi Zhang, Xinran Jiang, Ming Gong, Cunlong Lu, Ruru Pan, Shaoxing Chen","doi":"10.1007/s11274-024-04149-x","DOIUrl":"https://doi.org/10.1007/s11274-024-04149-x","url":null,"abstract":"<p><p>Extracellular proteases from haloarchaea, also referred to as halolysins, are in increasing demand and are studied for their various applications in condiments and leather industries. In this study, an extracellular protease encoding gene from the haloarchaeon Halorubellus sp. PRR65, hly65, was cloned and heterologously expressed in E. coli. The novel halolysin Hly65 from the genus Halorubellus was characterized by complete inhibition of phenylmethanesulfonyl fluoride (PMSF) on its enzyme activity. Experimental determination revealed a triad catalytic active center consisting of Asp¹⁵⁴-His¹⁹³-Ser³⁴⁸. Deletion of the C-terminal extension (CTE) resulted in loss of enzyme activity, while dithiothreitol (DTT) did not inhibit the enzyme activity, suggesting that Hly65 may function as a monomer. The K_m, V_max and K_cat for the Hly65 were determined to be 2.91 mM, 1230.47 U·mg^-1 and 1538.09 S^-1, respectively, under 60 °C, pH 8.0 and 4.0 M NaCl using azocasecin as a substrate. Furthermore, a three-dimensional structure prediction based on functional domains was obtained in this study which will facilitate modification and reorganization of halolysins to generate mutants with new physiological activities.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and quantification of peptaibol produced by novel Trichoderma spp: Harnessing their potential to mitigate moisture stress through enhanced biochemical and physiological responses in black pepper (Piper nigrum L.).","authors":"Vijayasanthi Kodakkal Valiyambath, Titty Anna Thomas, Priya George, Leela Neettiyath Kalathil, Anees Kaprakkaden, Krishnamurthy Kuntagodu Subraya, Dinesh Raghavan, Praveena Ravindran","doi":"10.1007/s11274-024-04131-7","DOIUrl":"https://doi.org/10.1007/s11274-024-04131-7","url":null,"abstract":"<p><p>Trichoderma spp. is primarily applied to manage biotic stresses in plants. Still, they also can mitigate abiotic stresses by the stimulation of antioxidative protective mechanisms and enhanced synthesis of secondary metabolites. The study optimized the conditions to enhance peptaibol production by novel Trichoderma spp, characterized and quantified peptaibol- alamethicin using HPLC and LC MS-MS. The present study investigated these isolates efficacy in enhancing growth and the associated physio-biochemical changes in black pepper plants under moisture stress. Under in vitro conditions, out of 51 isolates studied, six isolates viz., T. asperellum (IISR NAIMCC 0049), T. erinaceum (IISR APT1), T. harzianum (IISR APT2), T. harzianum (IISR KL3), T. lixii (IISR KA15) and T. asperellum (IISR TN3) showed tolerance to low moisture levels (5, 10 and 20%) and higher temperatures (35 and 40 °C). In vivo evaluation on black pepper plants maintained under four different moisture levels (Field capacity [FC]; 75%, 50%, and 25%) showed that the plants inoculated with Trichoderma accumulated greater quantities of secondary metabolites viz., proline, phenols, MDA and soluble proteins at low moisture levels (50% and 25% FC). In the present study, plants inoculated with T. asperellum and T. harzianum showed significantly increased growth compared to uninoculated plants. The shortlisted Trichoderma isolates exhibited differences in peptaibol production and indicated that the peptide might be the key factor for their efficiency as biocontrol agents. The present study also demonstrated that Trichoderma isolates T. harzianum and T. asperellum (IISR APT2 & NAIMCC 0049) enhanced the drought-tolerant capabilities of black pepper by improving plant growth and secondary metabolite production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}