World journal of microbiology & biotechnology最新文献

A psychrotolerant extracellular phosphatase from Krossfjorden sediment bacterium Bacillus cereus KR_O9: purification and functional characterization.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-14 DOI: 10.1007/s11274-025-04252-7

E S Lisintta, Annie George, Sona Gafoor, S Vishnupriya, Jesmi Yousuf, Bhavya Kachiprath, A K Valsa, Ally C Antony, P A Aneesa, A A Mohamed Hatha

{"title":"A psychrotolerant extracellular phosphatase from Krossfjorden sediment bacterium Bacillus cereus KR_O9: purification and functional characterization.","authors":"E S Lisintta, Annie George, Sona Gafoor, S Vishnupriya, Jesmi Yousuf, Bhavya Kachiprath, A K Valsa, Ally C Antony, P A Aneesa, A A Mohamed Hatha","doi":"10.1007/s11274-025-04252-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04252-7","url":null,"abstract":"This study reports the identification of a phosphatase-producing psychrotolerant bacterium, Bacillus cereus KR_O9, isolated from Arctic Krossfjorden sediments. The extracellular KR_O9 phosphatase was purified to homogeneity through acetone precipitation and ion exchange chromatography, achieving a specific activity of 12.51 U mg⁻1 min⁻1, a 17.3-fold purification, and a 64.94% yield. The molecular mass of the enzyme was estimated as 37 kDa by SDS-PAGE. Km, and Vmax for KR_O9 phosphatase towards substrate p-NPP were determined as 0.033 M L-1, 1.67 mg-1 min-1. The enzyme exhibited optimal activity at 40 °C and pH 6.0, with stability up to ≤ 40 °C and at pH levels ≤ 7.0, highlighting its thermolability and sensitivity to alkaline pH. At 10 mM level, Cu2+, Hg2+, Co2+, Pb2+, urea, EDTA, and GSH significantly stimulated the enzyme, and Ca2+, Zn2+, Mn2+, SDS, and CTAB were, strong inhibitors. The kinetic efficiency, resilience to low to neutral pH, and tolerance to various metal ions, including heavy metals, underscore the potential of B. cereus KR_O9 phosphatase for bioremediation and biofertilizer applications, paving the way for industrial exploration.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"72"},"PeriodicalIF":4.0,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene knockout studies of Dps protein reveals a novel role for DNA-binding protein in maintaining outer membrane permeability.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-13 DOI: 10.1007/s11274-025-04269-y

Indu Pant, Akhilesh A Potnis, Ravindranath Shashidhar

{"title":"Gene knockout studies of Dps protein reveals a novel role for DNA-binding protein in maintaining outer membrane permeability.","authors":"Indu Pant, Akhilesh A Potnis, Ravindranath Shashidhar","doi":"10.1007/s11274-025-04269-y","DOIUrl":"10.1007/s11274-025-04269-y","url":null,"abstract":"DNA-binding proteins like Dps are crucial for bacterial stress physiology. This study investigated the unexpected role of Dps protein in maintaining outer membrane integrity of Salmonella Typhimurium. We observed that a Δdps mutant displayed increased sensitivity to glycopeptide antibiotics (vancomycin, nisin), which are ineffective against Gram-negative bacteria due to their thick outer membrane (OM). Furthermore, the Δdps mutant exhibited susceptibility to membrane-disrupting agents like detergents (deoxycholate, SDS) and phages. The perforation was observed in OM after the treatment of vancomycin using atomic force microscopy. Notably, this sensitivity was rescued by supplementing the media with calcium and magnesium cations. These findings suggest a novel function for Dps in maintaining outer membrane permeability. We propose two potential mechanisms: 1) Dps might directly localize to the outer membrane 2) Dps might regulate genes responsible for lipopolysaccharide synthesis or outer membrane proteins, key components of outer membrane. This study highlights a previously unknown role for Dps beyond DNA binding and warrants further investigation into the precise mechanism by which it influences outer membrane integrity in Salmonella.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"70"},"PeriodicalIF":4.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenosiderophores: bridging the gap in microbial iron acquisition strategies.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-13 DOI: 10.1007/s11274-025-04287-w

Ravinsh Kumar, Ashutosh Singh, Amrita Srivastava

{"title":"Xenosiderophores: bridging the gap in microbial iron acquisition strategies.","authors":"Ravinsh Kumar, Ashutosh Singh, Amrita Srivastava","doi":"10.1007/s11274-025-04287-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04287-w","url":null,"abstract":"Microorganisms acquire iron from surrounding environment through specific iron chelators known as siderophores that can be of self-origin or synthesized by neighboring microbes. The latter are termed as xenosiderophores. The acquired iron supports their growth, survival, and pathogenesis. Various microorganisms possess the ability to utilize xenosiderophores, a mechanism popularly termed as 'siderophore piracy' besides synthesizing their own siderophores. This adaptability allows microorganisms to conserve energy by reducing the load of siderogenesis. Owing to the presence of xenosiderophore transport machinery, these microbial systems can be used for targeting antibiotics-siderophore conjugates to control pathogenesis and combat antimicrobial resistance. This review outlines the significance of xenosiderophore utilization for growth, stress management and virulence. Siderogenesis and the molecular mechanism of its uptake by related organisms have been discussed vividly. It focuses on potential applications like disease diagnostics, drug delivery, and combating antibiotic resistance. In brief, this review highlights the importance of xenosiderophores projecting them beyond their role as mere iron chelators.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"69"},"PeriodicalIF":4.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular structural arrangement in quorum sensing and bacterial metabolic production.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-13 DOI: 10.1007/s11274-025-04280-3

Victor U Chigozie, Morteza Saki, Charles O Esimone

{"title":"Molecular structural arrangement in quorum sensing and bacterial metabolic production.","authors":"Victor U Chigozie, Morteza Saki, Charles O Esimone","doi":"10.1007/s11274-025-04280-3","DOIUrl":"https://doi.org/10.1007/s11274-025-04280-3","url":null,"abstract":"Quorum sensing (QS) regulates bacterial behaviors such as biofilm formation, virulence, and metabolite production through signaling molecules like acyl-homoserine lactones (AHLs), peptides, and AI-2. These signals are pivotal in bacterial communication, influencing pathogenicity and industrial applications. This review explores the molecular architecture of QS signals and their role in metabolite production, emphasizing structural modifications that disrupt bacterial communication to control virulence and enhance industrial processes. Key findings highlight the development of synthetic QS analogs, engineered inhibitors, and microbial consortia as innovative tools in biotechnology and medicine. The review underscores the potential of molecular engineering in managing microbial behaviors and optimizing applications like biofuel production, bioplastics, and anti-virulence therapies. Additionally, cross-species signaling mechanisms, particularly involving AI-2, reveal new opportunities for regulating interspecies cooperation and competition. This synthesis aims to bridge molecular insights with practical applications, showcasing how QS-based technologies can drive advancements in microbial biotechnology and therapeutic strategies.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"71"},"PeriodicalIF":4.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillomics as a new notion in lactic acid bacteria research through omics integration.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-11 DOI: 10.1007/s11274-025-04285-y

Özge Kahraman Ilıkkan

{"title":"Lactobacillomics as a new notion in lactic acid bacteria research through omics integration.","authors":"Özge Kahraman Ilıkkan","doi":"10.1007/s11274-025-04285-y","DOIUrl":"10.1007/s11274-025-04285-y","url":null,"abstract":"Omics technologies are a set of disciplines that analyze large-scale molecular data to understand biological systems in a holistic way. These technologies aim to reveal the structure, functions and interactions of organisms by studying processes at many levels of biomolecules, from the genome to metabolism. Lactobacillomics is introduced as an interdisciplinary field that integrates multiple \"omics\" technologies-including genomics, transcriptomics, proteomics, metabolomics, and metagenomics- to provide a comprehensive insight into \"lactic acid bacteria\" species. Lactobacillomics aims to elucidate the genetic, metabolic, and functional characteristics of lactic acid bacteria (LAB) species, providing insights into the mechanisms underlying their probiotic effects and contributions to the host microbiome. By analyzing genomes and metabolic pathways, researchers can identify specific genes responsible for health-promoting functions and desirable fermentation characteristics, which can guide the development of targeted probiotic strains with optimized health benefits. The integration of these omics data allows facilitating the discovery of biomarkers for health and disease states, the development of new probiotics tailored to specific populations or health conditions, and the optimization of fermentation processes to enhance the safety, flavor, and nutritional profile of fermented foods. A comprehensive review and bibliometric analysis were conducted to provide an overview of this promising field between 2005 and 2025 by examining Web of Science Core Collection data. Research results reveal trending topics, future perspectives, and key areas of growth within lactic acid bacteria (LAB) studies, particularly as they intersect with omics technologies.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"68"},"PeriodicalIF":4.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for cost-optimized biocement production: a comprehensive review.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-08 DOI: 10.1007/s11274-025-04281-2

Zhen Yan, Kazunori Nakashima, Chikara Takano, Satoru Kawasaki

引用次数: 0

Microbial production systems and optimization strategies of antimicrobial peptides: a review.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-08 DOI: 10.1007/s11274-025-04278-x

Mengxue Lou, Shuaiqi Ji, Rina Wu, Yi Zhu, Junrui Wu, Jiachao Zhang

{"title":"Microbial production systems and optimization strategies of antimicrobial peptides: a review.","authors":"Mengxue Lou, Shuaiqi Ji, Rina Wu, Yi Zhu, Junrui Wu, Jiachao Zhang","doi":"10.1007/s11274-025-04278-x","DOIUrl":"https://doi.org/10.1007/s11274-025-04278-x","url":null,"abstract":"Antibiotic resistance has become a public safety issue of the twenty-first century, posing a growing threat and drawing increased attention. Compared to traditional antibiotics, antimicrobial peptides (AMPs), as naturally produced small peptides, can target multiple pathways within pathogens and render them less prone to developing resistance. This makes them promising alternatives to antibiotics. However, traditional chemical synthesis methods face challenges, such as high costs, low yields, and poor stability, limiting the large-scale industrial production of AMPs. Despite extensive research to improve AMP production efficiency, issues such as low yields and complex extraction processes continue to pose significant barriers to commercial application. Therefore, there is an urgent need for new biosynthesis strategies and optimization methods to enhance AMP production efficiency and quality. This review summarizes the sources, classification, mechanisms of action and recent advances in the microbial synthesis of AMPs. It also explores innovative production methods, including recombinant microbial expression systems, fusion tags, codon optimization, tandem multimer expression, and hybrid peptide expression. Furthermore, we review the applications of gene editing technologies and artificial intelligence in AMP production, providing new perspectives and strategies for efficient, large-scale AMP production.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"66"},"PeriodicalIF":4.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of aromatic amino acids and their derivatives by Escherichia coli and Corynebacterium glutamicum.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-07 DOI: 10.1007/s11274-025-04264-3

Takashi Hirasawa, Yasuharu Satoh, Daisuke Koma

引用次数: 0

Association between intraspecific variability and penicillin production in industrial strain, Penicillium chrysogenum revealed by RAPD and SRAP markers.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-06 DOI: 10.1007/s11274-025-04277-y

Yu Jong Ri, Chang Ho Ri, Un Hyang Ho, Sam Rang Song, Il Sim Pak, Tae Ryong Ri, Yong Jo Kim, Jun Song Ri

{"title":"Association between intraspecific variability and penicillin production in industrial strain, Penicillium chrysogenum revealed by RAPD and SRAP markers.","authors":"Yu Jong Ri, Chang Ho Ri, Un Hyang Ho, Sam Rang Song, Il Sim Pak, Tae Ryong Ri, Yong Jo Kim, Jun Song Ri","doi":"10.1007/s11274-025-04277-y","DOIUrl":"https://doi.org/10.1007/s11274-025-04277-y","url":null,"abstract":"Penicillin represents antibiotic discovered for the first time that prevents bacterial infections. Production of penicillin using penicillin-producing fungi (Penicillium chrysogenum) mainly depends on activity of industrial strain, optimization of culture condition and purification efficiency. To contribute to management of penicillin-producing strains, we conducted phylogenetic study on 27 P. chrysogenum variants originated from industrial strain and selected on media supplemented with penicillin or phenylacetic acid (PAA) using 4 Random Amplified Polymorphic DNA (RAPD) primers and 2 Sequence-Related Amplified Polymorphic (SRAP) primer pairs. The UPGMA dendrogram distinguished 27 variants into 2 clusters at the genetic distance of 0.3, consistent with classification by penicillin titers of variants and supported by principal component analysis (PCA) and STRUCTURE analysis. These results suggest effectiveness of RAPD and SRAP markers in management of P. chrysogenum variants exhibiting different penicillin titers and may contribute to increase in penicillin production by enabling inoculation of confirmed industrial strain exhibiting high penicillin productivity at the beginning of culture.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"64"},"PeriodicalIF":4.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovations and challenges in collagen and gelatin production through precision fermentation.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-02-06 DOI: 10.1007/s11274-025-04276-z

Sofia de Moura Campos, Gabriela Dos Santos Costa, Susan Grace Karp, Vanete Thomaz-Soccol, Carlos Ricardo Soccol

{"title":"Innovations and challenges in collagen and gelatin production through precision fermentation.","authors":"Sofia de Moura Campos, Gabriela Dos Santos Costa, Susan Grace Karp, Vanete Thomaz-Soccol, Carlos Ricardo Soccol","doi":"10.1007/s11274-025-04276-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04276-z","url":null,"abstract":"Collagen and gelatin are essential biomaterials widely used in industries such as food, cosmetics, healthcare, and pharmaceuticals. Traditionally derived from animal tissues, these proteins are facing growing demand for more sustainable and ethical production methods. Precision fermentation (PF) offers a promising alternative by using genetically engineered microorganisms to produce recombinant collagen and gelatin. This technology not only reduces environmental impact but also ensures consistent quality and higher yields. In this review, we provide a comprehensive overview of collagen and gelatin production through PF destined for the food sector, exploring key advances in recombinant technologies, synthetic biology, and bioprocess optimization. Challenges such as scaling production, cost-efficiency, and market integration are addressed, alongside emerging solutions for enhancing industrial competitiveness. We also highlight leading companies leveraging PF to drive innovation in the food industry. As PF continues to evolve, future developments are expected to improve efficiency, reduce costs, and expand the applications of recombinant collagen and gelatin, particularly in the food and supplement sectors.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"63"},"PeriodicalIF":4.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0