World journal of microbiology & biotechnology最新文献

The use of isomerases and epimerases for the production of the functional sugars mannose, allulose and tagatose from Fructose. 利用异构酶和表聚合酶从果糖生产功能糖甘露糖、异戊糖和塔格糖。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-09 DOI: 10.1007/s11274-025-04344-4

Junya Yang, Yingying Zhu, Xu Wei, Dawei Ni, Wenli Zhang, Wanmeng Mu

{"title":"The use of isomerases and epimerases for the production of the functional sugars mannose, allulose and tagatose from Fructose.","authors":"Junya Yang, Yingying Zhu, Xu Wei, Dawei Ni, Wenli Zhang, Wanmeng Mu","doi":"10.1007/s11274-025-04344-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04344-4","url":null,"abstract":"Fructose, a common monosaccharide in nature extensively utilized in the food industry, poses a risk of elevated chronic disease incidence with excessive consumption. The global movement for a healthier living has sparked a quest for sugar reduction in foodstuff. The growing concern regarding the adverse impact of excessive sugar consumption on public health has led to significant interest in investigating healthier sugar alternatives. Research efforts have refocused on converting fructose into high-value, reduced-calorie functional sugars. Fructose can undergo direct conversion into three such sugars-mannose, allulose, and tagatose-via a streamlined bioconversion process. Allulose and tagatose, epimers of fructose, are derivable directly from fructose through C-3 and C-4 epimerization processes, whereas mannose, the aldose isomer of fructose, can be synthesized via isomerization pathways. This article aims to present recent advancements in the physiological functions, production methods, and applications of functional sugars derived from fructose. Particularly, it focuses on the bioproduction of mannose, allulose, and tagatose from fructose, encompassing discussions on the recent progress in the related isomerases and epimerases, such as mannose isomerase/lyxose isomerase, ketose 3-epimerase, and tagatose 4-epimerase. This review will provide a fresh perspective on the high-value biological utilization of fructose resources.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"129"},"PeriodicalIF":4.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal dynamics and enzyme profiles of diverse endophytic fungi in Sterculia urens Roxb.: insights into host-associated trends. Sterculia urens Roxb.中多种内生真菌的季节动态和酶特征：洞察寄主相关趋势。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-09 DOI: 10.1007/s11274-025-04339-1

Garima Yadav, Mukesh Meena

{"title":"Seasonal dynamics and enzyme profiles of diverse endophytic fungi in Sterculia urens Roxb.: insights into host-associated trends.","authors":"Garima Yadav, Mukesh Meena","doi":"10.1007/s11274-025-04339-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04339-1","url":null,"abstract":"Sterculia urens Roxb., well known for its medicinal uses, remains largely unexplored in terms of its fungal endophytic communities. This study is the first comprehensive assessment of fungal endophyte diversity in S. urens. Sampling was conducted across different plant parts, seasons, and study sites. Molecular identification of fungal isolates was carried out using ITS sequencing. Additionally, colonization frequency and endophytic fungal diversity were analyzed. These isolates were evaluated for extracellular enzyme. A total of 31 different endophytic fungal species, representing 16 genera, were identified based on > 97% ITS sequence similarity. Colonization frequency was significantly influenced by season (P ≤ 0.0001), tissue type (P ≤ 0.0001), and site (P ≤ 0.0001). Diversity indices revealed a significant difference in relation to season and tissue type, but not with respect to location. Furthermore, this study reports, for the first time globally, the identification of Chaetomium meridiolense and Crinipellis wandoensis as endophytic fungi. Extracellular enzyme analysis revealed enzymatic activity in 29 morphotypes. The diverse enzymatic profiles of these fungal endophytes highlight their potential for various biotechnological applications. In addition, molecular and genomic investigations will provide a deeper understanding of the functional roles and symbiotic mechanisms of these fungal endophytes. Potential applications of this research include enhancing plant growth and stress tolerance, developing sustainable biofertilizers and biocontrol agents, promoting eco-friendly bioremediation strategies for dye-contaminated environments, and discovering novel enzymes suitable for industrial biotechnological processes.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"128"},"PeriodicalIF":4.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kraft lignin decomposition by lignin-derived aromatic compound degrader Rhodococcus sp. DK17.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-07 DOI: 10.1007/s11274-025-04350-6

Dockyu Kim, Mincheol Kim, Han-Woo Kim, Eungbin Kim, Hyoungseok Lee

{"title":"Kraft lignin decomposition by lignin-derived aromatic compound degrader Rhodococcus sp. DK17.","authors":"Dockyu Kim, Mincheol Kim, Han-Woo Kim, Eungbin Kim, Hyoungseok Lee","doi":"10.1007/s11274-025-04350-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04350-6","url":null,"abstract":"Rhodococcus sp. DK17 has been previously isolated from oil-contaminated soil and studied for its ability to degrade various monocyclic alkylbenzenes. This study investigated the decomposition of kraft lignin (genes, enzymes, and metabolic pathways) by DK17, using whole-genome sequencing data, as a potential biocatalyst for biotechnological lignin valorization. DK17 used kraft lignin and its main degradative metabolites, such as vanillin and vanillic acid, as growth substrates. High-performance liquid chromatography revealed that DK17 converted dehydrodivanillin (a representative lignin model compound). Quantitative polymerase chain reaction of mRNAs from DK17 cells induced in the presence of lignin showed that the putative genes coding for two copies of dye-decolorizing peroxidases (dypB1 and dypB2) were upregulated 1.6- and 2.4-fold after 5 and 24 h of induction, respectively, compared with glucose-induced cells. Vanillic acid induced dypB1 and dypB2 at lower levels than lignin by 1.4- and 1.6-fold after 5 and 24 h of induction, respectively. Computational homology analysis using the DypB1 and DypB2 protein sequences also predicted their initial roles in lignin decomposition. The duplicated dyp genes are believed to allow DK17 to achieve prolonged and continuous initial lignin decomposition, cleaving C-C and C-O-C linkages in the main lignin structure, the arylglycerol-β-aryl ether. Based on the above data, DK17 appears to initiate oxidative lignin decomposition using DyPs, producing smaller metabolites, such as vanillin and vanillic acid, which could be accumulated as value-added bioproducts (in metabolically engineered mutant strains) or further degraded for cell growth (in wild-type strains) via an ortho-ring cleavage pathway.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"127"},"PeriodicalIF":4.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Native Rhizobium biofertilization enhances yield and quality in Solanum lycopersicum under field conditions. 在田间条件下，本地根瘤菌生物肥料提高了茄果类作物的产量和质量。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-07 DOI: 10.1007/s11274-025-04349-z

Adriana Gen-Jiménez, José David Flores-Félix, Clara Ivette Rincón-Molina, Luis Alberto Manzano-Gómez, Juan José Villalobos-Maldonado, Nancy Ruiz-Lau, Rocio Roca-Couso, Víctor Manuel Ruíz-Valdiviezo, Reiner Rincón-Rosales

{"title":"Native Rhizobium biofertilization enhances yield and quality in Solanum lycopersicum under field conditions.","authors":"Adriana Gen-Jiménez, José David Flores-Félix, Clara Ivette Rincón-Molina, Luis Alberto Manzano-Gómez, Juan José Villalobos-Maldonado, Nancy Ruiz-Lau, Rocio Roca-Couso, Víctor Manuel Ruíz-Valdiviezo, Reiner Rincón-Rosales","doi":"10.1007/s11274-025-04349-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04349-z","url":null,"abstract":"In response to growing concerns about the environmental and economic impacts of chemical fertilizers, this study explores the potential of biofertilization using native Rhizobium strains to enhance the growth, yield, and quality of Solanum lycopersicum (tomato) under field conditions. The experiment assessed the effects of Rhizobium biofertilization on plant performance and soil microbial communities by applying R. calliandrae, R. jaguaris, R. mayense, and a bacterial consortium, in comparison to conventional chemical fertilization. Key parameters such as plant height, fruit yield, macronutrient and micronutrient content, and fruit quality (lycopene and β-carotene levels) were measured. Results showed that R. calliandrae and R. jaguaris significantly enhanced fruit yield, nitrogen, potassium, manganese, and boron levels, while also improving fruit quality compared to the control. The impact of strain inoculation on the structure of the microbial community was also examined. Metataxonomic analysis of rhizospheric soils revealed no significant changes in microbial diversity, indicating that biofertilization with Rhizobium strains promotes plant growth without disrupting the composition of the soil microbiome. These findings suggest that Rhizobium biofertilization is a viable and sustainable alternative to chemical fertilizers, providing benefits to both crop productivity and soil health while minimizing the environmental footprint associated with conventional agricultural practices. The study underscores the importance of carefully selecting bacterial species with complementary functions to maximize the effectiveness of biofertilization strategies.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"126"},"PeriodicalIF":4.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PGPB-driven bioenrichment and metabolic modulation of Salicornia europaea under marine Aquaponic conditions.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-07 DOI: 10.1007/s11274-025-04335-5

Maria J Ferreira, Erika Garcia-Cardesín, I Natalia Sierra-Garcia, Diana C G A Pinto, Javier Cremades, Helena Silva, Ângela Cunha

{"title":"PGPB-driven bioenrichment and metabolic modulation of Salicornia europaea under marine Aquaponic conditions.","authors":"Maria J Ferreira, Erika Garcia-Cardesín, I Natalia Sierra-Garcia, Diana C G A Pinto, Javier Cremades, Helena Silva, Ângela Cunha","doi":"10.1007/s11274-025-04335-5","DOIUrl":"10.1007/s11274-025-04335-5","url":null,"abstract":"This study analyzed the secondary metabolite profile of Salicornia europaea inoculated with Brevibacterium casei EB3 and Pseudomonas oryzihabitans RL18 in aquaponic systems, exploring the metabolic mechanisms responsible for the observed shifts. Experiments were conducted in both microcosm and pilot-scale aquaponic setups to evaluate how these metabolic shifts vary across different system scales and their potential contributions to the observed increased accumulation of bioactive compounds with antioxidant and antimicrobial properties, including some phenolic acids, such as caffeic acid (154-fold), flavonoids (2.85-fold), and some unsaturated fatty acids, such as oct-3-enoic acid (32-fold). Metabolic profiling revealed shifts in pathways associated with plant growth and stress resilience, such as amino acid and phenolic biosynthesis. Additionally, differences in metabolic responses observed between microcosm and pilot-scale systems underscored the importance of understanding scaling effects. These findings highlight the potential for optimizing aquaponic systems by leveraging microbial-plant interactions to enhance ecological and economic outcomes. This approach offers valuable applications in nutrient recycling, phytopharmaceutical development, and the advancement of saline agriculture within integrated aquaculture frameworks.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"124"},"PeriodicalIF":4.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfate-reducing bacteria in removal of pollutants: a promising candidate for bioremediation.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-07 DOI: 10.1007/s11274-025-04345-3

Panchami Sreeja Shaji, Salom Gnana Thanga Vincent, Karthikeyan Subburamu

{"title":"Sulfate-reducing bacteria in removal of pollutants: a promising candidate for bioremediation.","authors":"Panchami Sreeja Shaji, Salom Gnana Thanga Vincent, Karthikeyan Subburamu","doi":"10.1007/s11274-025-04345-3","DOIUrl":"https://doi.org/10.1007/s11274-025-04345-3","url":null,"abstract":"Industrial processes contribute significantly to environmental pollution, particularly by releasing sulfate-rich wastewater containing toxic metals and organic pollutants. Sulfate-reducing bacteria (SRB), being anaerobic microorganisms, are capable of reducing sulfate to sulfide, which precipitates harmful heavy metals and facilitates bioremediation. This review explores the potential of SRB in industrial wastewater treatment, focusing on their roles in the bioremediation of sulfates, heavy metals, and persistent organic pollutants (POPs). Laboratory-scale experiments demonstrated that SRB effectively reduces sulfate concentrations and removes heavy metals such as zinc, cadmium, and chromium through sulfidogenesis. The treatment process shows promise as an eco-friendly alternative to conventional chemical methods. However, challenges related to hydrogen sulfide emissions and process scalability persist. Future research focuses on enhancing SRB activity through optimized bioreactor designs while effectively controlling H2S release. This review emphasizes SRB as a promising candidate for industrial applications in wastewater treatment and environmental management.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"125"},"PeriodicalIF":4.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a nitrifying bacterial community for a low temperature recirculating aquaculture system.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-03 DOI: 10.1007/s11274-025-04341-7

Jiro Arima, Takumi Matsumoto, Haruki Nagamura, Rikuo Tsukamoto, Hirokazu Haga, Katsuhiko Shimizu

{"title":"Development of a nitrifying bacterial community for a low temperature recirculating aquaculture system.","authors":"Jiro Arima, Takumi Matsumoto, Haruki Nagamura, Rikuo Tsukamoto, Hirokazu Haga, Katsuhiko Shimizu","doi":"10.1007/s11274-025-04341-7","DOIUrl":"10.1007/s11274-025-04341-7","url":null,"abstract":"Two separate ammonia- and nitrite-oxidizing bacterial communities were developed to operate in a low temperature closed recirculating aquaculture system. These communities were cultivated via batch culture using an inorganic nutrient medium containing ammonia or nitrite. Subsequently, a unique closed recirculating culture system was developed, and enrichment culture was performed in an inorganic nutrient medium containing 1 mM ammonia. Through this approach, a bacterial community was developed that can efficiently nitrify 1 mM ammonia within 1 day at 15 °C. Amplicon sequencing revealed Nitrosomonadaceae and Nitrospirales, were the key groups responsible for ammonia and nitrite oxidation. The bacterial community was introduced into microbial tanks for the rearing of Oryzias latipes var. himedaka and Lefua echigonia (Hotokedojo) at 15 °C, where regular measurements confirmed the effective removal of ammonia and nitrite. However, nitrate accumulation occurred, which was mitigated by the introduction of Epipremnum aureum (Pothos) into the tank. This system provides a sustainable solution for the closed recirculating aquaculture of cold-water fish species.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"123"},"PeriodicalIF":4.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metataxonomic identification of microorganisms and sensory attributes of Coffea canephora under conventional processing and Self-Induced Anaerobiosis Fermentation.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-02 DOI: 10.1007/s11274-025-04340-8

Patrícia Campos Bernardes, Denes Kaic Alves do Rosário, Pedro Henrique Alves Martins, Rosane Freitas Schwan

{"title":"Metataxonomic identification of microorganisms and sensory attributes of Coffea canephora under conventional processing and Self-Induced Anaerobiosis Fermentation.","authors":"Patrícia Campos Bernardes, Denes Kaic Alves do Rosário, Pedro Henrique Alves Martins, Rosane Freitas Schwan","doi":"10.1007/s11274-025-04340-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04340-8","url":null,"abstract":"This study evaluates for the first time the modifications in the microbial communities and sensory attributes caused by Self-Induced Anaerobiosis Fermentation (SIAF) compared to the Conventional processing of Coffea canephora var. Conilon. Microorganisms were identified through high-throughput sequencing of the 16S rRNA V3/V4 region for bacteria and the ITS region for fungi. Sensory attributes of roasted coffee were evaluated by Q-Graders. The relationship between microbial population, processing methods, and sensory attributes was investigated using principal component analysis. Before fermentation, 74 bacterial and 21 fungal species were identified in the natural coffee, whereas 44 bacterial and 15 fungal species were found in the pulped coffee. Torulaspora, Wickerhamomyces, and Meyerozyma exhibited more ITS region sequences, while Acetobacter, Enterobacter, and Lysinibacillus were predominant in the 16S region. In the natural coffee, Wickerhamomyces showed the highest relative abundance (45%) at 0 h. After 72 h, Meyerozyma (45%) and Torulaspora (75%) prevailed in Conventional processing and SIAF, respectively. In the pulped coffee, Torulaspora was the most abundant in the SIAF method, before (92%) and after (81%) fermentation, while Wickerhamomyces (39%) dominated after 72 h in the Conventional method. Enterobacteriaceae levels decreased, while Lactobacillaceae levels increased in SIAF natural coffee during the fermentation process. SIAF favored the presence of yeast and LAB while inhibiting mycotoxigenic fungi and Enterobacteriaceae. Torulaspora, Lactiplantibacillus, and Lactococcus showed the highest Pearson correlation coefficient with flavor (0.92), aftertaste (0.99), and bitterness/sweetness (0.89), respectively. Changes in coffee microbiota during SIAF improved sensory attributes, resulting in better-quality beverages.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"122"},"PeriodicalIF":4.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome analysis and biocontrol potential of endophytic Bacillus cereus EMS1 against Fusarium wilt in banana.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-01 DOI: 10.1007/s11274-025-04326-6

Shilpi Singh, Piyush Kant Rai, Azmat Ali Khan, Sabiha Fatima, Kamlesh Choure, Jeong Chan Joo, Ashutosh Pandey

{"title":"Whole genome analysis and biocontrol potential of endophytic Bacillus cereus EMS1 against Fusarium wilt in banana.","authors":"Shilpi Singh, Piyush Kant Rai, Azmat Ali Khan, Sabiha Fatima, Kamlesh Choure, Jeong Chan Joo, Ashutosh Pandey","doi":"10.1007/s11274-025-04326-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04326-6","url":null,"abstract":"Endophytic bacteria are essential for promoting plant growth and increasing plant resilience to various environmental stresses. Although it is well-documented that several endophytic Bacillus species exhibit plant growth-promoting properties, this is the first report on the genome study of Bacillus cereus EMS1, isolated from Musa acuminata G9 in India. This study analyzed the genomics, plant growth traits, and fusarium wilt mitigation potential of Bacillus cereus EMS1. This analysis identified specific genomic features, including potential mechanisms contributing to plant growth promotion, which were also submitted to NCBI (Bioproject ID: PRJNA784269). The in vivo study showed that EMS1 mitigated the impact of Fusarium oxysporum f. sp. cubense on banana plants. Although it did not affect the number of leaves, other parameters influenced by pathogen infection and EMS1 treatment showed notable differences, including fresh weight (Fusarium oxysporum only: 15 g; EMS1 + Fusarium oxysporum: 21 g), dry weight (Fusarium oxysporum only: 1 g; EMS1 + Fusarium oxysporum: 4.7 g), and root length (Fusarium oxysporum only: 6.5 cm; EMS1 + Fusarium oxysporum: 9 cm). Additionally, genomic analysis revealed that the EMS1 genome contains distinctive genes linked to plant growth and antimicrobial activity. Overall, the findings highlight the potential of endophytic Bacillus cereus EMS1 in promoting plant growth and enhancing banana plant resistance against Fusarium oxysporum.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"119"},"PeriodicalIF":4.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of AbrB from plate to bioreactor: implications of induced expression on physiological and metabolic responses in Bacillus thuringiensis.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-04-01 DOI: 10.1007/s11274-025-04334-6

Shirlley Elizabeth Martínez Tolibia, Adrián Díaz Pacheco, Miguel Ángel Villalobos López, Rita Karen Pacheco Cabañas, Rosina Cabrera, Jorge Rocha, Víctor Eric López Y López

{"title":"The role of AbrB from plate to bioreactor: implications of induced expression on physiological and metabolic responses in Bacillus thuringiensis.","authors":"Shirlley Elizabeth Martínez Tolibia, Adrián Díaz Pacheco, Miguel Ángel Villalobos López, Rita Karen Pacheco Cabañas, Rosina Cabrera, Jorge Rocha, Víctor Eric López Y López","doi":"10.1007/s11274-025-04334-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04334-6","url":null,"abstract":"Transition state regulators from Bacillus can control diverse physiological responses such as growth, metabolism, motility, virulence, and sporulation. The AbrB protein is a transcriptional regulator involved in multiple functions during exponential phase and intricated regulatory pathways that control adaptive states differentially. Despite its importance, the AbrB role has not been well characterized during the growth cycle, and its implication in metabolic functions remains elusive, especially in the Bacillus cereus group. In this work, we characterized the role of AbrB on phenotypes such as spreading motility, growth profiles, sporulation, and on activity of core metabolic pathways of Bacillus thuringiensis. For this, a strain with inducible abrB expression was generated in the wild type Bt HD73 background. In vitro evaluations of phenotypic traits demonstrated differences in sporulation and motility, where induction of abrB presumably affected these functions under nutrient-limited media. In addition, AbrB induction during bioreactor fermentations led to higher biomass production and changes dissolved oxygen (DO) profile, which was also accompanied with a delay in sporulation. Based on these results, metabolic pathways such as glycolysis and the Krebs cycle were explored to address the effect of AbrB overproduction on transcription of genes coding for pyruvate dehydrogenase (pdHA), lactate dehydrogenase (ldH), citrate synthase (citZ) and aconitase (citB). Our findings suggest variations in the carbon-flux in the central carbon metabolism due to abrB overexpression. This work contributes to the elucidation of AbrB involvement in regulatory networks of B. thuringiensis, to develop engineering-based strategies to use these bacteria in other biotechnological applications besides as biological control agent.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"120"},"PeriodicalIF":4.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0