World journal of microbiology & biotechnology最新文献

{"title":"Modification and applications of glucose oxidase: optimization strategies and high-throughput screening technologies.","authors":"Zeyang Li, Yong Chen, Xihua Chen, Zhongjian Guo, Guoqiang Guan, Yong Feng, Huayou Chen","doi":"10.1007/s11274-025-04475-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04475-8","url":null,"abstract":"<p><p>Glucose oxidase (GOD), an oxidoreductase (EC 1.1.3.4), catalyzes the oxidation of β-D-glucose to gluconic acid using molecular oxygen as the electron acceptor, with concomitant generation of hydrogen peroxide. Owing to its versatile catalytic properties, GOD has garnered significant attention across diverse fields, including food and beverage manufacture, agriculture, biosensors and biotechnology. However, the inherent limitations of native enzymes, including susceptibility to inactivation under harsh conditions and insufficient catalytic efficiency, restrict their practical utility in advanced industry. This review systematically summarizes recent advances in molecular engineering strategies for GOD optimization, focusing on rational design and directed evolution approaches to improve its functional robustness and application adaptability in the bioeconomy. Furthermore, we highlight the prospective role of artificial intelligence (AI) and machine learning (ML) in addressing the classical activity-stability trade-off, enabling data-driven prediction of mutation hotspots and dynamic regulation of enzymatic properties. By integrating computational biology with experimental validation, this work proposes a theoretical framework and technical roadmap for developing \"tailored\" GOD variants that meet precise industrial requirements. The insights presented herein aim to bridge the gap between fundamental enzyme research and scalable biomanufacturing, fostering innovation in sustainable biotechnology.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"266"},"PeriodicalIF":4.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144620773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycelium-based products: multifunctional flexible mycomaterial, a textile alternative produced from fungi and lignocellulose waste.","authors":"Bruna Pertile, Roselei Claudete Fontana, Renan Isoton, Rosmary Nichele Brandalise, Marli Camassola","doi":"10.1007/s11274-025-04470-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04470-z","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"265"},"PeriodicalIF":4.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144620774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing microbial nanobiotics: Lactobacillus extracellular vesicles as next-generation therapeutics across physiological systems.","authors":"Ke Peng, Lina Liu, Shujun Gao","doi":"10.1007/s11274-025-04481-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04481-w","url":null,"abstract":"<p><p>Lactobacillus-derived extracellular vesicles (LEVs) are emerging as pivotal mediators of interkingdom communication, carrying strain-specific bioactive cargo that influences diverse physiological systems, including the gut, nervous, respiratory, reproductive, and integumentary systems. These nanoscale structures demonstrate significant therapeutic potential, modulating host immunity, enhancing barrier integrity, combating pathogens, and contributing to tissue repair across numerous health contexts. However, realizing their full clinical and biotechnological promise faces substantial hurdles. Key challenges include variability in isolation and purification methods, inconsistencies in LEV yield and composition due to cultivation conditions and inherent strain differences, and the observation of context-dependent or even paradoxical effects in certain diseases. Furthermore, gaps persist in understanding their precise biogenesis, cargo sorting mechanisms, and long-term biodistribution. Future progress necessitates interdisciplinary efforts to standardize production and characterization, resolve mechanistic ambiguities, and develop robust bioengineering strategies for targeted delivery and enhanced functionality. Addressing these obstacles is crucial to harness LEVs effectively as versatile tools in precision medicine, microbiome engineering, and sustainable applications in health and industry.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"261"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brucellosis in the omics era: integrative perspectives on Brucella genomic architecture, host-pathogen interactions, and disease dynamics.","authors":"Shashi Bhushan Kumar, Giridhar Goudar, Manne Munikumar, Shanvanth R Arnipalli, Puneeta Singh Yaduvanshi, Virendra V Panpatil","doi":"10.1007/s11274-025-04484-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04484-7","url":null,"abstract":"<p><p>Brucellosis is a widespread and persistent zoonotic disease caused by Gram-negative Brucella species, posing significant threats to human health, livestock, and economic stability. This review provides a comprehensive insight into current knowledge on Brucella pathogenicity, genomic structure and therapeutic challenges. We highlight the host-specific pathogenic mechanism of predominated species as B. melitensis, B. abortus, B. suis and B. canis focusing on their bipartite genome organization, virulence factors and strategies for intracellular survival and immune evasion. Diagnostic approaches including serological assays, such as SAT, ELISA, and molecular techniques like PCR molecular techniques are critically evaluated, alongside current antibiotic regimens and their limitations due to resistance and relapse. We further discuss promising developments in vaccine research-such as live attenuated, subunit, and DNA-based platforms-as well as adjunctive therapies involving nanotechnology, herbal compounds, and novel antibiotics. Emphasis is placed on the integration of genomic insights with therapeutic innovation. We conclude by identifying key research priorities, including the need for improved diagnostics, resistance profiling, and next-generation vaccines. This review underscores the importance of a multidisciplinary approach to mitigate the global burden of brucellosis and advance translational research in prevention and treatment.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"264"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosorption and bioremediation of heavy metal ions from wastewater using algae: A comprehensive review.","authors":"Khushbu S Parmar, Kalpesh M Patel","doi":"10.1007/s11274-025-04424-5","DOIUrl":"https://doi.org/10.1007/s11274-025-04424-5","url":null,"abstract":"<p><p>Algal-based bioremediation has emerged as a promising, environmentally sustainable, and economically feasible method for the removal of heavy metal ions from contaminated wastewater. This review offers a comprehensive examination of the potential of both microalgae and macroalgae in biosorption and bioremediation processes. It investigates the physicochemical mechanisms involved, including surface adsorption, ion exchange, complexation, bioaccumulation, bioprecipitation, and enzymatic reduction. The efficiency of metal removal is influenced by various factors such as algal species, metal type, pH, and temperature. Case studies underscore the successful application of algae in removing metals such as lead, cadmium, arsenic, and chromium. The advantages of using algae include high metal-binding capacity, rapid growth rates, biomass regeneration potential, and minimal sludge generation. However, challenges persist in biomass harvesting, reusability, and scaling up for real-world applications. Future advancements may focus on the genetic engineering of algal strains and integration with other treatment technologies to enhance performance and feasibility in industrial settings.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"262"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can probiotics be protective and growth-promoting to coffee plants?","authors":"Guilherme Peixoto de Freitas, Flávia Rodrigues Alves Patricio, Rafaela Carvalho Vargas, Wagner Bettiol","doi":"10.1007/s11274-025-04465-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04465-w","url":null,"abstract":"<p><p>Probiotics show well-documented benefits to animal and human health and are safe for organisms and the environment. This study explored the potential of five commercial probiotics - Colostrum^® BS Liquid, Colostrum^® BS Powder, Colostrum^® BIO 21 Liquid, and Colostrum^® BIO 21 MIX Powder (widely used in poultry production), and Enterogermina^® (used for humans) in managing coffee leaf rust (CLR), brown eye spot (BES), bacterial leaf blight (BHB) and dieback, as well as their effect on coffee seedlings growth. In vitro experiments revealed that only Colostrum^® BIO 21 Liquid completely inhibited urediniospores germination of Hemileia vastatrix, CLR causal agent, while the other probiotics showed partial inhibition. Colostrum^® BS Powder, partially inhibited Cercospora coffeicola (BES causal agent) and Boeremia coffea (dieback causal agent) mycelial growth. Colostrum^® BS Powder and Colostrum^® BIO 21 MIX Powder, containing Bacillus sp. and lactic acid bacteria, significantly reduced the severity of CLR, dieback and showed some effectiveness against BHB (caused by Pseudomonas syringae pv. garcae). None of the tested probiotics reduced BES severity. Beyond disease control, Colostrum^® BS Powder and Colostrum^® BIO 21 MIX Powder consistently enhanced coffee seedling growth parameters, including shoot and root biomass, root volume, and leaf characteristics, in both cell trays and rhizotrons. Conversely, liquid formulations and Enterogermina^® generally did not show significant benefits for disease control or plant growth promotion. These findings suggest that Colostrum^® BS Powder and Colostrum^® BIO 21 MIX Powder, that are safe for the environment and have their mass production well developed, could offer a promising, eco-friendly approach to enhancing plant health and coffee seedling growth. After further studies in field conditions they can become important tools to improve sustainable coffee production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"260"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the lysine deacetylases gene and its dynamic expression profile during adversity stress and infestation in Arthrinium phaeospermum.","authors":"Sijia Liu, Ziqi Ye, Jia Song, Yutong Liu, Shujiang Li","doi":"10.1007/s11274-025-04479-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04479-4","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"263"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microcella aerolata GA224 exhibits preventive potential against Streptococcus pneumoniae infection via the gut-lung axis.","authors":"Ningqianzi Tang, Yimin Pan, Zicheng Jin, Guoxia Zhang","doi":"10.1007/s11274-025-04478-5","DOIUrl":"https://doi.org/10.1007/s11274-025-04478-5","url":null,"abstract":"<p><p>Antibiotics and vaccines have long been major key interventions against Streptococcus pneumoniae (Spn) infection. However, alternative therapies are urgently needed with the original therapies becoming suboptimal efficacy. A beneficial bacterium, Microcella aerolata strain GA224, with protective potential against Spn infection was isolated in previous study. Here, this protective effect was investigated at the bacterial, cellular and animal levels, exploring the mechanisms from the perspective of the gut-lung axis. Cellular and animal models of Spn infection were established and GA224 was administered for prevention or treatment. Spn adherence, inflammatory gene expression, histopathological features, gut microbial profiles and fecal metabolomic signatures were examined. In vitro, GA224 inhibited Spn growth with a bacteriostatic diameter of 16 mm and reduced adherence by 83.4% (P < 0.01) in prevention groups, while suppressing inflammatory gene expression (IL-1β, IL-6, TNF-α) by 40-50% (P < 0.001) in Spn-infected cells. In Spn-infected rats, GA224 intranasal administration improved body weight gain by 8.2% (vs. Tre group + 3.7%, P < 0.05) and reduced lung injury score by 25% (P < 0.01). In addition, GA224 administration alleviated dysbiosis of the gut microbiota, including declined abundance of Ruminiclostridium and Roseburia, increased abundance of Ruminococcus and restricted out-migration of infectious bacteria. In terms of the fecal metabolism, differential metabolites and disordered metabolic pathways were altered by GA224, including glycine, serine and threonine metabolism and pentose and glucuronate interconversion. Intestinal bacteria showed multiple correlations with fecal metabolites with strongest correlation founding between Ruminococcus and oxidized phospholipids. Notably, Anaerotaenia (r = -0.67 with LysoPC), Ruminiclostridium (positively correlated with lung coefficient, r = 0.62) and Ruminococcus (strongest correlation with oxidized phospholipids, r = 0.8) demonstrated microbiota-metabolite interactions potentially mediating gut-lung axis regulation.Finally, we demonstrate that M. aerolata GA224 provided a protective potential against Spn infection. In addition to the inhibition in adherence and inflammation, remodeling gut microbiota and improving metabolism via the gut-lung axis may be the critical avenue to this protective effect.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"259"},"PeriodicalIF":4.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Shiga toxin-producing Escherichia Coli O157 in Moroccan food and antimicrobial resistance profiles of the strains isolated.","authors":"Bouchra Ouarroud, Mohammed El Maadoudi, Lamyaa El Mamoun, Ayoub Kounnoun, Amina Barakat","doi":"10.1007/s11274-025-04477-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04477-6","url":null,"abstract":"<p><p>Foods contaminated with Shiga toxin-producing Escherichia coli (STEC) are implicated in serious outbreaks. The present study investigated the prevalence of stx1, stx2, eae, and rfbEO157 genes by real-time PCR in enrichment broths of ground beef, artisanal dairy products, lettuce, spinach, turkey, chicken and bivalve mollusks sold in northern Morocco, the isolation of STEC O157 from positive enrichment broths and determination of antimicrobial resistance profiles of the isolates. The screening results revealed that amongst 620 samples analyzed, 82 enrichment broths (13.23%) were stx + (stx1 or/and stx2) eae- rfbEO157-, 77 out of 620 enrichment broths (12.42%) were stx + eae + rfbEO157-, and 73 out of 620 enrichment broths (11.77%) were stx + eae + rfbEO157 + . The food matrix most contaminated was ground beef 33/140 (23.57%), followed by dairy 27/140 (19.29%), poultry 8/140 (5.71%), leafy vegetables 4/100 (4.00%) and then bivalve mollusks 1/100 (1.00%). 14 STEC O157 isolates were isolated from 4 positive samples (4/73; 5.5%) (multiple isolates were recovered from each positive sample). Antibiotic susceptibility testing showed that the STEC O157 isolates were resistant to at least 4 of the 20 antimicrobials tested. All isolates were resistant to amoxicillin/clavulanic acid, 11/14 (78.6%) were resistant to streptomycin, 10/14 (71.4%) were resistant to tobramycin, 9/14 (64.3%) were resistant to ertapenem and piperacillin, 8/14 (57.1%) were resistant to cefotaxime and 7/14 (50.0%) were resistant to ampicillin and ceftazidime. All isolates were sensitive to amikacin, ceftazidime/avibactam, gentamicin, imipenem, nitrofurantoin and trimethoprim/sulfamethoxazole. Results indicate food producers and sellers may need further training in good hygiene rules, highlighting the importance of implementing strict quality control to ensure food safety.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"258"},"PeriodicalIF":4.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of aflatoxin in dried figs.","authors":"Senem Öztürk Köse, Hacı Halil Biyik","doi":"10.1007/s11274-025-04466-9","DOIUrl":"10.1007/s11274-025-04466-9","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"257"},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}