World journal of microbiology & biotechnology最新文献_第2页

Microbial diversity, metabolic properties, and biotechnological prospects of acid-sulfate soils. 酸性硫酸盐土壤微生物多样性、代谢特性及生物技术前景。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-08 DOI: 10.1007/s11274-025-04444-1

Mavi Johanna Blanco-Diaz, Valéria Maia Oliveira

{"title":"Microbial diversity, metabolic properties, and biotechnological prospects of acid-sulfate soils.","authors":"Mavi Johanna Blanco-Diaz, Valéria Maia Oliveira","doi":"10.1007/s11274-025-04444-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04444-1","url":null,"abstract":"Acid-sulphate soils (ASS) are worldwide naturally or anthropogenically formed soils that originate from the oxidation of pyrite in anoxic sulfide substrates. Classification of soils into ASS types is complex and usually depends on grain size, oxidation rates, pH values, acidification potential and typical microbial communities. Currently, there is not a consensual classification of ASS and definitions are not universally applied. The global use of ASS for crops and mining areas has become an environmental problem, raising concerns in the scientific community and prompting the study of microorganisms inhabiting this acidic environment and their potential for further use in bioremediation processes. Globally, ASS harbor a higher abundance of the bacterial phylum Pseudomonadota, especially members of the Beta and Gammaproteobacteria classes, followed by other less abundant phyla like Bacillota, Actinomycetota, Acidobacteriota, Bacteroidetes, Nitrospirota and Chloroflexota. Among the archaea reported in ASS are members of the genera Thermoproteus and Acidilobus and of the Thermoplasmata class. Bacteria and archaea found in the different types of ASS exhibit diverse metabolisms, including dissimilatory processes like iron and/or inorganic sulfur oxidation, iron or sulfate reduction, aluminum and iron reduction and methanogenesis, assimilatory processes like autotrophy, diazotrophy, and other metabolic properties such as phosphate solubilization and acidogenesis. This diversity offers a wide range of biotechnological applications, including bioremediation of crop areas and natural ecosystems affected by acidity and a vast field for basic and applied scientific research.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"256"},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monochromatic LeafAdaptNet (MLAN): an adaptive approach to spinach leaf disease detection using monochromatic imaging. 单色叶适应网络（MLAN）：一种利用单色成像进行菠菜叶病检测的自适应方法。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-08 DOI: 10.1007/s11274-025-04442-3

Meganathan Elumalai, Terrance Frederick Fernandez, R Kaviarasan, S Kannadhasan

{"title":"Monochromatic LeafAdaptNet (MLAN): an adaptive approach to spinach leaf disease detection using monochromatic imaging.","authors":"Meganathan Elumalai, Terrance Frederick Fernandez, R Kaviarasan, S Kannadhasan","doi":"10.1007/s11274-025-04442-3","DOIUrl":"https://doi.org/10.1007/s11274-025-04442-3","url":null,"abstract":"A country's economic growth heavily relies on agricultural productivity, specifically nutrition derived from vegetables and leafy greens. Spinach, abundant in iron, vitamins, and other essential nutrients, plays a vital role in maintaining the health of human tissues, cartilage, and hair. However, extreme summer heat and plant diseases can significantly reduce spinach yields, making it less nutritious and harder to obtain. Implementing improved detection and classification of bacterial and fungal diseases affecting spinach leaves is crucial for minimizing pesticide use and enhancing agricultural output. A cutting-edge approach was introduced for identifying diseases in spinach leaves through deep learning object detection. To tackle these issues, the DenseNet-121-DO model served as the basis for developing the Custom Monochromatic LeafAdaptNet (MLAN). Spinach leaves were classified as Half-Spinach, Curry Leaves, Drumstick Leaves, and Lettuce, with the aid of Google-Colaboratory. This model displayed impressive results, achieving an accuracy of 99.10% and a mean Average Precision (mAP) of 98.16%. Such outcomes promote higher agricultural productivity and reduced pesticide costs by showcasing the system's effectiveness in accurately identifying and classifying spinach leaf diseases.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"255"},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic changes in Phycomyces blakesleeanus mycelia during selenite reduction and cellular localization of synthesized SeNPs. 黑藻菌菌丝在亚硒酸盐还原和合成SeNPs的细胞定位过程中的代谢变化。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-08 DOI: 10.1007/s11274-025-04416-5

Ivanka Rodić, Milan V Žižić, Jovana Lukičić, Marina Stanić, Alessandra Gianoncelli, Valentina Bonanni, Joanna Zakrzewska, Miroslav Ž Živić, Tijana Cvetić Antić

{"title":"Metabolic changes in Phycomyces blakesleeanus mycelia during selenite reduction and cellular localization of synthesized SeNPs.","authors":"Ivanka Rodić, Milan V Žižić, Jovana Lukičić, Marina Stanić, Alessandra Gianoncelli, Valentina Bonanni, Joanna Zakrzewska, Miroslav Ž Živić, Tijana Cvetić Antić","doi":"10.1007/s11274-025-04416-5","DOIUrl":"https://doi.org/10.1007/s11274-025-04416-5","url":null,"abstract":"This study considers the capacity of fungus Phycomyces blakesleeanus for removal of toxic selenite from the environment and metabolic response of the mycelium during Se transformation. The X-ray Fluorescence Microscopy (XFM) suggests two pathways of selenite transformation: extensive internalisation and intracellular transformation leading to volatilization, and synthesis of SeNPs at the cell surface, with the contribution of each pathway depending on selenite concentration and treatment duration. Glutathione plays an important role in the reduction process, as the glutathione pool is alternately removed from and restored to redox balance during Se treatment. Enzymes facilitate the maintenance of the cellular redox balance, which is reflected in an increase in the specific activities of glutathione reductase, glutathione peroxidase, glutathione S-transferase and catalase at different time points during the 48-h exposure of mycelia to 100 µM selenite. During the transition from the exponential to the stationary growth phase, a metabolic shift was documented, which can be seen in the change of the total glutathione content and glutathione redox status. This points out that the developmental stage of the mycelia plays an important role in the capacity for selenite reduction and mycelia survival in a selenium-enriched medium. This work is a step towards the use of selenite-contaminated media for Se extraction and re-utilisation and suggests that Phycomyces blakesleeanus might be a suitable organism for the effective re-utilisation of Se.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"254"},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the impact of Curvularia pathogens on medicinal and aromatic plants: insights into history, pathogenicity, and host-pathogen interactions. 探索曲曲霉病原菌对药用和芳香植物的影响：对历史，致病性和宿主-病原体相互作用的见解。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-07 DOI: 10.1007/s11274-025-04421-8

Atul Kumar Srivastava, Mehran Khan, Xiangyang Li, Pooja Misra, Ashish, Akhil Kumar, Entaj Tarafder, Xin Xie, Raghvendra Pratap Singh

{"title":"Exploring the impact of Curvularia pathogens on medicinal and aromatic plants: insights into history, pathogenicity, and host-pathogen interactions.","authors":"Atul Kumar Srivastava, Mehran Khan, Xiangyang Li, Pooja Misra, Ashish, Akhil Kumar, Entaj Tarafder, Xin Xie, Raghvendra Pratap Singh","doi":"10.1007/s11274-025-04421-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04421-8","url":null,"abstract":"The genus Curvularia comprises globally distributed phytopathogenic fungi that predominantly infect grasses, including various Medicinal and Aromatic Plants (MAPs). Despite its ecological and agricultural significance, studies on the morphology, phenotypic diversity, and infection mechanisms of Curvularia in MAPs remains scarce. Taxonomic understanding of this genus has also evolved over time, with repeated revisions and reclassifications. This review presents a chronicle key discovery, taxonomic updates, and systematic advancements in Curvularia research. Known to severely impact the Poaceae family, staple crops, and MAPs (Rauvolfia, Ocimum spp., Aloe vera, and Curcuma spp.) Curvularia employ diverse genes to disrupt host metabolic pathways, inducing physiological dysfunctions disease symptoms. Here, we consolidate global reports on Curvularia-associated diseases in MAPs, analysing host-pathogen interactions across geographical regions. Furthermore, we explore the disease cycle, biochemical infection strategies, and molecular pathogenesis to elucidate fungal virulence. A dedicated focus is placed on Curvularia-derived secondary metabolites and toxins, highlighting their role in host manipulation and pathogenicity. By integrating these insights, this review aims to address critical knowledge gaps and direct future research on Curvularia-MAPs interactions.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"252"},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification. 用重组纤维生物水解酶（GH7）强化拉森氏菌分泌组，实现高效生物质糖化。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-07 DOI: 10.1007/s11274-025-04473-w

Yashika Raheja, Varinder Singh, Vivek Kumar Gaur, Gaurav Sharma, Adrian Tsang, Bhupinder Singh Chadha

{"title":"Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification.","authors":"Yashika Raheja, Varinder Singh, Vivek Kumar Gaur, Gaurav Sharma, Adrian Tsang, Bhupinder Singh Chadha","doi":"10.1007/s11274-025-04473-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04473-w","url":null,"abstract":"GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 could fill this gap and how it stacks up against the industrial workhorse strain Trichoderma reesei Cel7A, we codon optimized the R. emersonii gene (Rem_GH7CBHI), expressed it in Pichia pastoris and purified the recombinant enzyme for structural and functional analysis. The 57 kDa protein retains the canonical GH7 βsandwich tunnel, but an AlaforTyr substitution leaves the channel more open than that of T. reesei, potentially easing substrate entry. Consistent with this architecture, Rem_GH7CBHI binds cellotriose tightly and exhibits a low Kₘ of 0.25 mM. Biochemical characterization revealed the optimal activity at pH 5.0, 60 °C and retaining about 60% activity after 1 h at 70 °C. Adding Rem_GH7CBHI together with an endogenous AA9 LPMO to the native R. emersonii secretome (M36) boosted saccharification of steamacidpretreated rice straw to levels comparable with commercial Cellic CTec3 at the same total protein loading. These results position Rem_GH7CBHI as a thermostable, highaffinity alternative to T. reesei Cel7A and a costeffective addition to tailored enzyme cocktails for highsolids lignocellulose biorefineries.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"253"},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of microbial community structures in rhizosphere soils of different plants in riverine wetland. 河流湿地不同植物根际土壤微生物群落结构比较

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-05 DOI: 10.1007/s11274-025-04423-6

Jiaying Zhao, Biao Liu, Yiran Hou, Liujie Zheng, Xixi Kong, Changrui Zhou, Junfeng Wu, Xiang Guo, Yimei Ying

{"title":"Comparison of microbial community structures in rhizosphere soils of different plants in riverine wetland.","authors":"Jiaying Zhao, Biao Liu, Yiran Hou, Liujie Zheng, Xixi Kong, Changrui Zhou, Junfeng Wu, Xiang Guo, Yimei Ying","doi":"10.1007/s11274-025-04423-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04423-6","url":null,"abstract":"Bacterial communities play an important role in nutrient cycling in riverine wetland ecosystems, and the study of rhizosphere microbial community composition of different plants is of great scientific significance in revealing the relationship between microorganisms and plant interactions in riverine wetlands. In this study, samples were collected from four plant congeries dominated by Artemisia argyi (AH), Acorus calamus (CP), Miscanthus sacchariflorus (DC), and Paspalum distichum (QB), respectively. High-throughput sequencing technology was employed to investigate the rhizosphere microbial community structures associated with these distinct plant species. The results showed that soil water content could effectively regulate the functional diversity of soil microbial communities. Different plant congeries types significantly affected soil bacterial community diversity, and at the phylum level, both Proteobacteria and Acidobacteria were absolutely dominant groups, followed by Bacteroidetes. At the genus level, Flavobacterium, Sphingomonas, Gp6 and Gp4 were the dominant bacteria in the rhizosphere soil of plants along the river. Soil pH, water content, NO3-N, NO2-N and TOC were the main factors affecting the composition of microbial communities. This study broadens our understanding of the composition of rhizosphere microbial communities in the river buffer zone, and provide data support for river ecological environment protection.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"250"},"PeriodicalIF":4.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic analysis of Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 responding to hyper- and hyposalinity stress. 南极海冰酵母黏胶红酵母AN5对高、低盐度胁迫响应的蛋白质组学分析。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-05 DOI: 10.1007/s11274-025-04472-x

Guangfeng Kan, Yan Zhang, Hong Zhang, Kai Yu, Yanxiao Jiang, Jie Jiang, Lina Qiao, Cuijuan Shi

{"title":"Proteomic analysis of Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 responding to hyper- and hyposalinity stress.","authors":"Guangfeng Kan, Yan Zhang, Hong Zhang, Kai Yu, Yanxiao Jiang, Jie Jiang, Lina Qiao, Cuijuan Shi","doi":"10.1007/s11274-025-04472-x","DOIUrl":"https://doi.org/10.1007/s11274-025-04472-x","url":null,"abstract":"Antarctic sea-ice microorganism lives in polar sea-ice channels, in which the salinity can be three times that of seawater in winter and close to freshwater in summer. Here, we investigated the survival and adaptive strategies of Antarctic yeast Rhodotorula mucilaginosa AN5 treated individually with high (150‰) and low (0‰) salinity to simulate the natural environment. The results showed that salinity changes did not significantly affect the growth of yeast AN5. Scanning electron microscope (SEM) observation revealed that the cell size increased under both hyper- and hyposalinity compared to control, and more extracellular substances were observed, especially under hypersalinity stress. Biochemical factors determination indicated that after high salinity exposure, the increased antioxidant systems eliminated the induced ROS, while under low salinity stress, ROS and the antioxidant system were decreased. The proteomic analysis based on iTRAQ and PRM technology revealed that 86 proteins were up-regulated and 388 were down-regulated. Under high salinity stress, the most predominant enrichment pathway was nucleocytoplasmic transport. In addition, high salinity exposure inhibited fatty acid metabolism, the one-carbon pool by folate, and ubiquitin-mediated proteolysis. Under low salinity stress, the glyoxylate cycle pathway was induced, and aldolase was down-regulated, indicating that the energy demand in yeast survival was supplied by the catabolism of lipids. Meanwhile, low salinity also limited the synthesis of RNA, DNA, and protein by inhibiting the ribosome biogenesis and pyrimidine metabolism pathways. Generally, these results provide comprehensive insights into the molecular mechanisms underlying the adaptation of Antarctic sea-ice yeast R. mucilaginosa to high and low salt stress.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"249"},"PeriodicalIF":4.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Pseudodesulfovibrio sp., a sulfate-reducing bacterium for the sustainable synthesis of cerium sulfide. 利用硫酸盐还原菌假脱硫弧菌可持续合成硫化铈。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-05 DOI: 10.1007/s11274-025-04462-z

Sonal Shete, Neelam Kapse, Prashant K Dhakephalkar

{"title":"Harnessing Pseudodesulfovibrio sp., a sulfate-reducing bacterium for the sustainable synthesis of cerium sulfide.","authors":"Sonal Shete, Neelam Kapse, Prashant K Dhakephalkar","doi":"10.1007/s11274-025-04462-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04462-z","url":null,"abstract":"The present study investigates a novel sulfate-reducing bacterium, Pseudodesulfovibrio sp. MCM B-508, isolated from produced water, for its ability to produce cerium sulfide, an industrially valuable rare earth colorant. The study demonstrates a microbial route for pigment synthesis by cloning and expressing dissimilatory sulfite reductase (dsrAB) genes from this bacterium in Escherichia coli. The enzyme dissimilatory sulfite reductase shared a maximum of 94.09% homology with published dissimilatory sulfite reductase sequences from the GenBank database, highlighting its novelty. Using molecular engineering techniques, including chaperone co-expression with the pGro7 vector, the sulfate conversion efficiency was significantly enhanced to 71.23%, overcoming limitations in sulfate-reducing bacterial processes. The research successfully converted cerium sulfate to the desired gamma-form cerium sulfide (Ce2S3) through a recombinant E. coli strain, achieving a more efficient and eco-friendly production method compared to conventional chemical synthesis approaches. X-ray diffraction analysis confirmed the formation of the targeted pigment, with major peaks at 25.3 and 32.8° 2θ, demonstrating the potential for an industrially viable, sustainable pigment production process.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"251"},"PeriodicalIF":4.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput screening identifies novel chemical scaffolds targeting Leishmania donovani parasites. 高通量筛选鉴定针对多诺瓦利什曼原虫寄生虫的新型化学支架。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-04 DOI: 10.1007/s11274-025-04441-4

Diksha Kumari, Vishwani Jamwal, Tashi Palmo, Anuj Kumar, Kuljit Singh

{"title":"High-throughput screening identifies novel chemical scaffolds targeting Leishmania donovani parasites.","authors":"Diksha Kumari, Vishwani Jamwal, Tashi Palmo, Anuj Kumar, Kuljit Singh","doi":"10.1007/s11274-025-04441-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04441-4","url":null,"abstract":"High-throughput screening (HTS) is one of the critical strategies that can accelerate the process of drug discovery by exploring small drug-like molecule libraries. HTS noticeably contributes to the identification of potential hits using various automation tools and biological assays. Leishmaniasis, a neglected tropical disease, is one of the most deadly protozoan parasitic infections causing significant global burden in certain poverty-stricken countries. Given the paucity of effective chemotherapeutic agents available for the treatment of this life-threatening infection, the discovery of novel leishmanicidal scaffolds through innovative strategies is pivotal to combating this parasitic disease. In the present study, we have employed the HTS strategy to scrutinize 10,000 drug-like molecules from the in-house ChemDiv library to identify potent hits against Leishmania donovani. Noticeably, 99 molecules showed > 80% inhibitory effect against the L. donovani parasites at 50 µM concentration, and among them, four molecules displayed a 50% inhibitory concentration (IC50) value < 10 µM along with a favorable selectivity index (> 10). Scanning electron microscopy analysis suggests that these potent hits cause considerable morphological alterations. The predicted ADME or pharmacokinetics study of these potent hit molecules indicates that all the hits have considerable drug-likeness properties and showed a low risk of adverse effects, along with a predicted high level of oral bioavailability. Thus, these promising hits identified by the HTS strategy can serve as an initial basis for consequent medicinal chemistry endeavors aimed at unraveling a new series of anti-leishmanial agents.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"247"},"PeriodicalIF":4.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of D-threitol from biomass-derived xylose by engineering Trichosporonoides oedocephalis. 生物质木糖合成d -苏糖醇的工程研究。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-04 DOI: 10.1007/s11274-025-04425-4

Jingyi Xu, Yue Tang, Yuqi Peng, Zhi Chen, Xin Ju, Liangzhi Li

{"title":"Biosynthesis of D-threitol from biomass-derived xylose by engineering Trichosporonoides oedocephalis.","authors":"Jingyi Xu, Yue Tang, Yuqi Peng, Zhi Chen, Xin Ju, Liangzhi Li","doi":"10.1007/s11274-025-04425-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04425-4","url":null,"abstract":"Xylose is the major pentose and the second most abundant sugar in the lignocellulosic feedstock. Metabolic engineering approaches have been employed to enhance the utilization rate of xylose in yeast. We previously modified Trichosporonoides oedocephalis ATCC 16958 and produced sugar alcohols, including xylitol and erythritol. D-threitol is a rare sugar that cannot be obtained directly from xylose conversion through fermentation. In this study, T. oedocephalis gained xylose assimilation activity by introducing ribulose-5-phosphate isomerase B from Ochrobactrum sp. and xylitol dehydrogenase from the yeast Scheffersomyces stipitis CBS 6054 for D-threitol production. Optimization of the composition of the medium ultimately led to an increase in the yield of D-threitol to 5.18 g/L. In addition, this study also analyzed the kinetics of the fermentation process, including cell growth, D-threitol biosynthesis and xylose consumption. Cell growth conformed to the logistics curve, and D-threitol synthesis and substrate consumption conformed to the Luedeking-Piret curve showing a growth-partially correlated type. This study demonstrated the potential of engineered yeast to synthesize high value-added products from biomass-derived xylose.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"248"},"PeriodicalIF":4.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0