World journal of microbiology & biotechnology最新文献_第2页

Fungal nano-factories: synthesis and characterization of silver nanoparticles from soil-borne fungus and their anti-microbial potential. 真菌纳米工厂：土壤真菌中银纳米颗粒的合成和表征及其抑菌潜力。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-26 DOI: 10.1007/s11274-025-04530-4

Insha Amin, Ruqeya Nazir, Mushtaq Ahmad Rather

引用次数: 0

Developing a highly efficient whole-cell biotransformation of 3,4-dihydroxyacetophenone into Apocynin by engineered Escherichia coli expressing caffeic acid O-methyltransferase. 利用表达咖啡酸o -甲基转移酶的工程大肠杆菌将3,4-二羟基苯乙酮高效全细胞转化为罗布麻素。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-14 DOI: 10.1007/s11274-025-04526-0

Wenyu Wang, Xiwei Yuan, Yalun Zhang, Yanqiu Tian, Dehong Xu

{"title":"Developing a highly efficient whole-cell biotransformation of 3,4-dihydroxyacetophenone into Apocynin by engineered Escherichia coli expressing caffeic acid O-methyltransferase.","authors":"Wenyu Wang, Xiwei Yuan, Yalun Zhang, Yanqiu Tian, Dehong Xu","doi":"10.1007/s11274-025-04526-0","DOIUrl":"10.1007/s11274-025-04526-0","url":null,"abstract":"Apocynin (Ap), a bioactive compound from the roots of Picrorhiza kurroa, faces challenges in production. This study developed a whole-cell biotransformation approach using engineered Escherichia coli expressing caffeic acid O-methyltransferase to convert 3,4-dihydroxyacetophenone into Ap. Caffeic acid O-methyltransferase from Medicago sativa (MsCOMT) showed the highest activity, yielding 90.75 mg·L⁻¹ and 97.04 mg·L⁻¹ Ap after 24 h and 48 h, while the mutant I319A enhanced titers to 198.32 mg·L⁻¹ and 228.37 mg·L⁻¹ by optimizing H269-D270 catalytic mechanisms. Besides, this study explored the impact of biotransformation conditions on the activity of MsCOMT and the yield of Ap. TB medium was found to be the most effective, with yields of 158.52 mg·L⁻¹ and 174.75 mg·L⁻¹ after 24 h and 48 h. The in vivo SAM regeneration system, less effective than in vitro SAM supplementation, still improved Ap yield when the genes mtn, luxS, and MsCOMT were arranged in a pseudo-operon configuration. Orthogonal experiments showed the importance order of transformation factors as: transformation temperature > induction temperature > substrate concentration > IPTG concentration, with optimal conditions being 35 °C transformation temperature, 15 °C induction temperature, 8 mmol·L⁻¹ substrate concentration, and 0.1 mmol·L⁻¹ IPTG concentration. Using mutant MsCOMTI319A under these optimal conditions, Ap yield increased steadily with transformation time, reaching a maximum of 544 mg·L⁻¹ after 72 h. This research successfully achieved the whole-cell biotransformation of 3,4-dihydroxyacetophenone into Ap for the first time, providing a foundation for further optimization of Ap biosynthesis.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"313"},"PeriodicalIF":4.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A critical review for removal of pre- and polyfluoroalkyl substances (PFAS) from landfill leachate: possible solutions with biological nitrogen removal and anammox systems. 从垃圾填埋场渗滤液中去除预氟烷基和多氟烷基物质（PFAS）：生物脱氮和厌氧氨氧化系统的可能解决方案。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-14 DOI: 10.1007/s11274-025-04529-x

Hassan Ramadan, Gamal Kamel Hassan, Zhaoji Zhang

{"title":"A critical review for removal of pre- and polyfluoroalkyl substances (PFAS) from landfill leachate: possible solutions with biological nitrogen removal and anammox systems.","authors":"Hassan Ramadan, Gamal Kamel Hassan, Zhaoji Zhang","doi":"10.1007/s11274-025-04529-x","DOIUrl":"10.1007/s11274-025-04529-x","url":null,"abstract":"Landfill leachate contains high concentrations of ammonia, organic nitrogen, and per- and polyfluoroalkyl substances (PFAS), which seriously threaten the surrounding ecosystem and human health. The potential degradation of PFAS within conventional nitrification-denitrification, heterotrophic nitrification-aerobic denitrification, and Anammox-mediated systems will be summarized with focusing on the responses of various microorganisms to PFAS stress in the current review. A thorough analysis of landfill leachate composition is essential for selecting the appropriate treatment systems and anticipating the hurdles associated with their implementation. This review will show the key reasons that can impede the effectiveness of anammox-mediated systems, such as leachate composition, ambient temperature, and operating conditions, and recommend control strategies to address these challenges. The knowledge gaps in current research, prospects, and the challenges that need to be addressed to achieve a sustainable and robust treatment approach for the simultaneous removal of nitrogen and PFAS from leachate will be highlighted. This review emphasizes the current biological systems for nitrogen removal from landfill leachate, with a specific focus on the anaerobic ammonium oxidation (Anammox) process and its innovative combinations as promising solutions for achieving highly efficient nitrogen removal performance, PFAS removal, reducing greenhouse gas (GHG) emissions, and minimizing energy consumption.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"311"},"PeriodicalIF":4.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable antifungal potential of ZnO and MoS₂ nanoparticles against Fusarium oxysporum and Fusarium graminearum. 氧化锌和二硫化钼纳米颗粒对尖孢镰刀菌和谷物镰刀菌的持续抗真菌潜力。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-14 DOI: 10.1007/s11274-025-04531-3

Marzieh Alikarami, Hossein Saremi, Mostafa Darvishnia

{"title":"Sustainable antifungal potential of ZnO and MoS2 nanoparticles against Fusarium oxysporum and Fusarium graminearum.","authors":"Marzieh Alikarami, Hossein Saremi, Mostafa Darvishnia","doi":"10.1007/s11274-025-04531-3","DOIUrl":"10.1007/s11274-025-04531-3","url":null,"abstract":"This study explores the antifungal potential of zinc oxide (ZnO) and molybdenum disulfide (MoS₂) nanoparticles (NPs) against Fusarium oxysporum and Fusarium graminearum, two major fungal pathogens threatening wheat production and grass pastures. Three sizes of ZnO NPs (30 nm, 200 nm, and 20 μm) and MoS₂ NPs (90 nm) were synthesized and characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, and Brunauer-Emmett-Teller (BET) analyses. Antifungal assays revealed that smaller ZnO NPs (30 nm) exhibited superior antifungal activity due to their high surface-to-volume ratio, achieving up to 79% inhibition of F. oxysporum, while MoS₂ NPs effectively inhibited F. graminearum growth by inducing oxidative stress and cellular damage, with a maximum inhibition rate of 83% (p < 0.05). The combination of ZnO and MoS₂ NPs demonstrated synergistic antifungal effects, as confirmed by light microscopy, which showed that ZnO NPs disrupted fungal cell wall integrity while MoS₂ NPs triggered oxidative stress and intracellular vacuolization. Greenhouse trials further validated the effectiveness of MoS₂ NPs in reducing Fusarium head blight (FHB) severity in wheat, underscoring their potential for sustainable wheat protection, with disease severity reduced by up to 35.8%. These findings highlight ZnO and MoS₂ NPs as promising eco-friendly alternatives to conventional fungicides, though further research is needed to optimize field applications, assess environmental impact, and integrate these NPs into comprehensive plant disease management strategies.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"312"},"PeriodicalIF":4.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic insight into drought-induced changes in the Egyptian wheat rhizosphere microbiome. 干旱诱导埃及小麦根际微生物组变化的宏基因组学研究。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-12 DOI: 10.1007/s11274-025-04518-0

Haytham M Abd El-Halim, Mohamed El-Hadidi, Nourhan Fouad, Ranin R Hamed, Islam A Megid, Manar H Taha, Khaled H Radwan

{"title":"Metagenomic insight into drought-induced changes in the Egyptian wheat rhizosphere microbiome.","authors":"Haytham M Abd El-Halim, Mohamed El-Hadidi, Nourhan Fouad, Ranin R Hamed, Islam A Megid, Manar H Taha, Khaled H Radwan","doi":"10.1007/s11274-025-04518-0","DOIUrl":"10.1007/s11274-025-04518-0","url":null,"abstract":"Wheat is one of the most important cereal crops and an important source of food for billions of people worldwide. However, drought stress can pose a real threat to its productivity and lead to significant yield losses, especially in Egypt. The rhizospheric microbiome of wheat can play an important role in drought stress and help wheat to respond to this abiotic stress. Understanding this microbiome is therefore also important to improve drought stress resilience and productivity. In this study, a metagenomic analysis was performed to investigate how the composition and diversity of microbial communities associated with the wheat rhizosphere change under drought. Taxonomic and phylogenetic analyses revealed a shift in microbial abundance, with Actinobacteria, Bacteroidetes, Proteobacteria and Verrucomicrobia being the four most abundant phyla of the ethnic microbiota. Remarkably, other classes, including Alphaproteobacteria and Cytophagia, were significantly enriched under drought, which could be a promising enhancement of plant stress altruism. Differential abundance analysis showed that the control samples had higher abundance of microbial taxa such as OD1, WS2, Chlorobi, ABY1 and SHA-109 compared to the drought-treated genotypes. Functional prediction analysis using PICRUSt showed that an uncharacterized ATP-binding protein within the AAA + superfamily is overrepresented under drought conditions. This suggests that these genes may play a role in stress adaptation, possibly via energy-dependent regulation of cellular processes involved in plant survival. Our results expand our understanding of the complexity of responses of the wheat rhizosphere microbiome to drought and have practical implications for the development of microbial target combinations to improve wheat tolerance and productivity in the context of climate change challenges.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"310"},"PeriodicalIF":4.2,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a β-N-acetylhexosaminidase CgNagase20 from Chaetomium globosum with antifungal activity against Fusarium sporotrichioides. 球毛菌β- n -乙酰己糖苷酶CgNagase20抗孢子毛枯菌活性的研究。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-11 DOI: 10.1007/s11274-025-04524-2

Cheng Jiang, Luli Li, Jialu Li

{"title":"Characterization of a β-N-acetylhexosaminidase CgNagase20 from Chaetomium globosum with antifungal activity against Fusarium sporotrichioides.","authors":"Cheng Jiang, Luli Li, Jialu Li","doi":"10.1007/s11274-025-04524-2","DOIUrl":"https://doi.org/10.1007/s11274-025-04524-2","url":null,"abstract":"Chitinolytic enzymes are capable of hydrolyzing the cell walls of pathogenic fungi, disrupting the exoskeletons of insect pests, and producing N-acetyl-β-D-glucosamine oligomers or monomers. In this study, a new β-N-acetylhexosaminidase gene encoding an enzyme from glycoside hydrolase family 20 (GH 20) was identified from the biocontrol fungus Chaetomium globosum W7. The corresponding protein was designated CgNagase20 and was heterologously expressed in Escherichia coli. The molecular mass of the protein was approximately 66.7 kDa, and this enzyme exhibited maximal activity at 55 °C and pH 4.0. Heavy metal ions, particularly Fe3+, Ag+, and Hg2+, were found to strongly inhibit CgNagase20 activity. CgNagase20 exhibits typical substrate specificity of β-N-acetylhexosaminidase (EC 3.2.1.52), acting on N-acetylglucosides and N-acetylgalactosides, with monosaccharides as the sole end products. The Vmax and Km values for CgNagase20 when acting on 4-nitrophenyl N-acetyl-β-D-glucosaminide (pNP-βGlcNAc) were calculated to be 118.85 µmol/min/mg protein and 1.59 mM, respectively. Additionally, CgNagase20 demonstrated antifungal activity, significantly inhibiting spore germination and hyphal growth of Fusarium sporotrichioides, the causative agent of potato dry rot. In conclusion, CgNagase20 represents the first β-N-acetylhexosaminidase identified and characterized from C. globosum, with potential applications in both the industrial and agricultural sectors.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"308"},"PeriodicalIF":4.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple detection and differentiation of Aeromonas caviae infection and contamination with specific monoclonal antibodies by dot blotting. 斑点印迹法检测和鉴别鼠腔气单胞菌感染和污染的特异性单克隆抗体。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-11 DOI: 10.1007/s11274-025-04522-4

Penpan Payattikul, Tiwa Rotchanapreeda, Pradit Wangman, Visut Rawiwet, Siwaporn Longyant

{"title":"Simple detection and differentiation of Aeromonas caviae infection and contamination with specific monoclonal antibodies by dot blotting.","authors":"Penpan Payattikul, Tiwa Rotchanapreeda, Pradit Wangman, Visut Rawiwet, Siwaporn Longyant","doi":"10.1007/s11274-025-04522-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04522-4","url":null,"abstract":"Aeromonas spp. are well-known pathogenic bacteria that cause severe infectious diseases in humans and animals through ingestion or contact with contaminated food and water; thus, detecting contamination in suspected samples is essential. This study developed a new detection tool by creating three strong monoclonal antibody (mAb) clones-AC-19, AC-4, and AC-16-by injecting mice with Aeromonas caviae antigens. The dot blot test using antigens from different gram-negative bacteria showed that AC-19 could detect all 88 samples of five Aeromonas species (A. caviae, Aeromonas hydrophila, Aeromonas sobria, Aeromonas veronii, and Aeromonas jandaei), but it also reacted with Plesiomonas shigelloides. Importantly, AC-4 and AC-16 each demonstrated 100% specificity to A. caviae and exhibited 100% sensitivity (31/31 isolates) and 64.52% sensitivity (20/31 isolates), respectively. These results suggest that AC-4 is the most promising mAb for A. caviae detection. Testing the limit of detection by dot blotting against A. caviae antigen exhibited the following. AC-19 at 3 × 107 CFU/ml, AC-4 at 1 × 107 CFU/ml, and AC-16 at 5 × 106 CFU/ml. Furthermore, their application was demonstrated by spiking tests for A. caviae contamination in contact lens samples, which proved their advantages in practical utilization. Thus, with high sensitivity and specificity, these mAbs constitute convenient immunological tools that could be used for simple, rapid, and simultaneous direct detection and differentiation of A. caviae and Aeromonas spp. in complex subjects, such as food and clinical samples, as well as infected animals, without the need for sophisticated bacterial separation, isolation, and biochemical characterization procedures.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"309"},"PeriodicalIF":4.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping the pangenome of sulfate reducing bacteria: core genes, plasticity, and novel functions in Desulfovibrio spp. 硫酸盐还原菌泛基因组图谱：核心基因、可塑性和新功能。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-09 DOI: 10.1007/s11274-025-04519-z

Shailabh Rauniyar, Dipayan Samanta, Payal Thakur, Priya Saxena, Ram N Singh, Adelme Bazin, Alain Bomgni, Ernest Fotseu, Gnimpieba Z Etienne, Venkata Gadhamshetty, Brent M Peyton, Matthew Fields, Mahadevan Subramaniam, Rajesh K Sani

{"title":"Mapping the pangenome of sulfate reducing bacteria: core genes, plasticity, and novel functions in Desulfovibrio spp.","authors":"Shailabh Rauniyar, Dipayan Samanta, Payal Thakur, Priya Saxena, Ram N Singh, Adelme Bazin, Alain Bomgni, Ernest Fotseu, Gnimpieba Z Etienne, Venkata Gadhamshetty, Brent M Peyton, Matthew Fields, Mahadevan Subramaniam, Rajesh K Sani","doi":"10.1007/s11274-025-04519-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04519-z","url":null,"abstract":"The pangenome of sulfate reducing bacteria represents a genetic reservoir that deciphers the intricate interplay of conserved and variable elements driving their ecological dominance, evolutionary adaptability, and industrial relevance. This study introduces the most comprehensive pangenome analysis of the genus Desulfovibrio till date, incorporating 63 complete and high-quality genomes using the Partitioned Pangenome Graph of Linked Neighbors (PPanGGOLiN) pipeline. The structure and dynamics of core gene families were investigated through gene ontology, KEGG pathway mapping, and gene network analyses, shedding light on the functional organization of the Desulfovibrio genomes. The analysis categorized 799, 4053, and 43,581 gene families into persistent, shell, and cloud groups, respectively. A core set of 326 gene families, conserved across Desulfovibrio genomes, highlights their essential role in community functionality. Genome plasticity analysis identified 4,576 regions of genome plasticity, with 1,322 hotspots enriched in horizontally acquired genes (89% in the cloud partition). Key gene families in these regions included glpE, fdhD, petC, and cooF, linked to sulfur metabolism. Out of 29 hypothetical genes, one was linked to actin nucleation, another contained a TRASH domain, while the other regulates filopodium assembly. Other predicted functions included lnrL, folE, RNA binding, and pyrG/pyrH involvement in CTP biosynthesis. Additionally, genomic islands revealed evolutionary events, such as cheY acquisition in Oleidesulfovibrio alaskensis G20. This study provides a genus-wide view of Desulfovibrio, emphasizing genome plasticity, hypothetical gene functions, and adaptation mechanisms.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"305"},"PeriodicalIF":4.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and distribution of arbuscular mycorrhizal fungi in phosphorus-deficient acidic soils of Northeast India: implications for sustainable agriculture. 印度东北部缺磷酸性土壤丛枝菌根真菌的多样性和分布：对可持续农业的影响。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-09 DOI: 10.1007/s11274-025-04516-2

Priya Murugesan, Pushpendra Sharma, Subrata Nath Bhowmik, Samik Chowdhury, Rajeev Kaushik

{"title":"Diversity and distribution of arbuscular mycorrhizal fungi in phosphorus-deficient acidic soils of Northeast India: implications for sustainable agriculture.","authors":"Priya Murugesan, Pushpendra Sharma, Subrata Nath Bhowmik, Samik Chowdhury, Rajeev Kaushik","doi":"10.1007/s11274-025-04516-2","DOIUrl":"https://doi.org/10.1007/s11274-025-04516-2","url":null,"abstract":"Soil acidity significantly impacts plant growth and yield, affecting approximately 90% of India's land, with 54% of acidic soils concentrated in the North-Eastern Region of India. Aluminium (Al) toxicity and phosphorus (P) deficiency coexist under acidic conditions, limiting agricultural productivity. Arbuscular Mycorrhizal Fungi (AMF) form symbiotic associations with plants, enhancing phosphorus uptake and mitigating Al toxicity. This study explores total and root-colonizing AMF diversity in acidic soils from undisturbed and cultivated sites in Mizoram (upland) and Tripura (lowland) using next-generation sequencing of amplicons from nested PCR with AMF-specific primers. Distinct variations in AMF communities were observed between undisturbed and cultivated sites in both regions. We identified 26 amplicon sequence variants (ASVs) in soil and six in roots, with Glomeraceae dominating. Cultivated sites in Mizoram showed the highest alpha diversity (Shannon: 2.17, Simpson: 0.84), with unique ASVs (e.g., Funneliformis, Diversispsora). Non-metric multidimensional scaling (NMDS) using Bray-Curtis dissimilarity showed greater AMF community similarity between undisturbed sites in Mizoram and Tripura. Soil parameters such as pH, EC, Al, and Cu significantly influenced AMF community composition. These findings provide crucial ecological insights into AMF communities in acidic soils, guiding the future development of targeted, AMF-based biofertilizers for sustainable crop production.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"303"},"PeriodicalIF":4.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Screen using Escherichia coli Keio knockout mutant line reveals genes related to the antimicrobial properties of trans-Cinnamic Acid. 利用大肠杆菌Keio敲除突变株进行全基因组筛选，揭示了反式肉桂酸抗菌特性相关基因。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-09 DOI: 10.1007/s11274-025-04506-4

Merve Sezer Kürkçü, Kadriye Aslıhan Onat Taşdelen, Hatice Öztürkel Kabakaş, Esra Dibek, Anara Babayeva, Emine Sonay Elgin, Bekir Çöl

{"title":"Genome-Wide Screen using Escherichia coli Keio knockout mutant line reveals genes related to the antimicrobial properties of trans-Cinnamic Acid.","authors":"Merve Sezer Kürkçü, Kadriye Aslıhan Onat Taşdelen, Hatice Öztürkel Kabakaş, Esra Dibek, Anara Babayeva, Emine Sonay Elgin, Bekir Çöl","doi":"10.1007/s11274-025-04506-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04506-4","url":null,"abstract":"Trans-cinnamic acid (tCA), a naturally occurring phenolic compound with antimicrobial activity, has poorly understood molecular mechanisms governing bacterial sensitivity and resistance. This study employed a genome-wide screen of Escherichia coli K-12 Keio single-gene knockout library (3,985 mutants) to identify genes modulating tCA response. Mutants were screened on LB medium supplemented with tCA (0-1.5 mg/mL). Phenotypic analysis identified 78 sensitive and 52 resistant mutants, validated through visual and quantitative assessments. Sequential spot assays under tCA stress confirmed classifications: sensitive mutants (e.g., ∆aaeX, ∆aaeA, ∆seqA, ∆vacJ, ∆dksA) displayed growth inhibition, while resistant mutants (e.g., ∆yhfK, ∆hofQ, ∆ybaT, ∆groL) exhibited enhanced growth. Mutants were categorized into High, Moderate, or Low Sensitivity/Resistance groups using integrated data from SGA Tools, genome-wide screening (GWS), and spot testing, yielding 18 High Sensitive (HS), 43 Moderate Sensitive (MS), 17 Low Sensitive (LS), 20 High Resistant (HR), 23 Moderate Resistant (MR), and 9 Low Resistant (LR) strains. Complementation of sensitive mutants (e.g., ∆dksA, ∆seqA, ∆aaeA, ∆vacJ) with wild-type alleles via plasmids restored growth and elevated minimum inhibitory concentrations (MICs), directly linking these genes to tCA sensitivity. Functional evaluation (EcoCyc, DAVID, STRING) revealed HS gene hits were associated with transcriptional regulation, metabolic activity, protein folding, DNA repair, transport, and membrane stability. Resistant gene hits were linked to stress response and detoxification pathways. This systems-level analysis elucidates the genetic basis of E. coli's response to tCA, identifying targets for antimicrobial strategies leveraging tCA or its derivatives.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"307"},"PeriodicalIF":4.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0