World journal of microbiology & biotechnology最新文献_第2页

Uptake of lead, cadmium and copper by heavy metal-resistant Pseudomonas aeruginosa strain DR7 isolated from soil. 从土壤中分离出的抗重金属铜绿假单胞菌 DR7 菌株对铅、镉和铜的吸收。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-21 DOI: 10.1007/s11274-024-04194-6

Jianhui Zhang, Zainura Zainon Noor, Nurul Huda Baharuddin, Siti Aminah Setu, Mohd Amir Asyraf Mohd Hamzah, Zainul Akmar Zakaria

{"title":"Uptake of lead, cadmium and copper by heavy metal-resistant Pseudomonas aeruginosa strain DR7 isolated from soil.","authors":"Jianhui Zhang, Zainura Zainon Noor, Nurul Huda Baharuddin, Siti Aminah Setu, Mohd Amir Asyraf Mohd Hamzah, Zainul Akmar Zakaria","doi":"10.1007/s11274-024-04194-6","DOIUrl":"https://doi.org/10.1007/s11274-024-04194-6","url":null,"abstract":"This study highlights the biosorption capacity for Cd (II), Cu (II) and Pb (II) by a locally isolated Pseudomonas aeruginosa DR7. At initial concentrations of 150 mg L-1 and 240 min of contact time, P. aeruginosa DR7 showed a 62.56 mg/g removal capacity for Cd (II) at an optimum pH of 6.0, 72.49 mg/g for Cu (II) at an optimum pH of 6.0, and 94.2 mg/g for Pb (II) at an optimum pH of 7.0. The experimental data of Cd (II), Cu (II), and Pb (II) adsorbed by the pseudo-second-order kinetic model correlates well with P. aeruginosa DR7, with R2 all above 0.99, showing that the fitting effect was satisfactory. The isothermal adsorption processes of Cd (II) (0.980) and Cu (II) (0.986) were more consistent with the Freundlich model, whereas Pb (II) was more consistent with the Langmuir model (0.978). FTIR analysis suggested the involvement of hydroxyl, carbonyl, carboxyl, and amine groups present in the inner regions of P. aeruginosa cells during the biosorption process. SEM-EDS analysis revealed that after contact with metals, there were slight changes in the surface appearance of the cells, which confirmed the deposition of metals on the bacterial surface. There was also the possibility of the metals being translocated into the bacterial inner regions by the appearance of electron-dense particles, as observed using TEM. As a conclusion, the removal of metals from solutions using P. aeruginosa DR7 was a plausible alternative as a safe, cheap, and easily used biosorbent.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"387"},"PeriodicalIF":4.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competition-driven phenotypic plasticity in Iron acquisition and aromatic utilization confers a fitness advantage to Pseudomonas putida in an Iron-limited rhizospheric environment. 铁获取和芳香利用的竞争驱动表型可塑性使假单胞杆菌在铁有限的根瘤环境中具有适应优势。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-20 DOI: 10.1007/s11274-024-04192-8

Hiren Joshi, Atif Khan

{"title":"Competition-driven phenotypic plasticity in Iron acquisition and aromatic utilization confers a fitness advantage to Pseudomonas putida in an Iron-limited rhizospheric environment.","authors":"Hiren Joshi, Atif Khan","doi":"10.1007/s11274-024-04192-8","DOIUrl":"10.1007/s11274-024-04192-8","url":null,"abstract":"Iron scarcity poses a critical challenge for rhizospheric bacteria like Pseudomonas putida in the competitive rhizosphere. Despite its dependence on iron for essential functions such as root colonization, motility, and aromatic compound utilization, P. putida exhibits limited capability for heterologous siderophore utilization and primarily relies on the secretion of a single siderophore, pyoverdine. This study investigates the mechanisms by which P. putida acquires iron in an iron-limited, aromatic-rich, rhizosphere-like environment. Our findings demonstrate that P. putida exhibits significant phenotypic plasticity, dynamically modulating pyoverdine secretion in response to competitive pressures and substrate availability. This adaptive strategy optimizes energy expenditure and iron acquisition, providing a competitive advantage. Comparative gene expression analysis supports these observations, revealing the molecular underpinnings of this plasticity. Enhanced pyoverdine production driven by competition compensates for the bacterium's limited siderophore repertoire and facilitates rapid aromatic compound utilization, conferring a distinct fitness advantage in iron-deprived conditions. This study elucidates the complex interplay between competition, iron uptake, and aromatic compound utilization that underpins the rhizospheric success of P. putida.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"386"},"PeriodicalIF":4.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and purification of a novel calcium-independent thermostable, α-amylase produced by Bacillus licheniformis UDS-5. 地衣芽孢杆菌 UDS-5 产生的新型钙依赖性恒温 α 淀粉酶的优化和纯化。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-19 DOI: 10.1007/s11274-024-04188-4

Sadikhusain Suthar, Disha Joshi, Harsh Patel, Darshan Patel, Bhavtosh A Kikani

{"title":"Optimization and purification of a novel calcium-independent thermostable, α-amylase produced by Bacillus licheniformis UDS-5.","authors":"Sadikhusain Suthar, Disha Joshi, Harsh Patel, Darshan Patel, Bhavtosh A Kikani","doi":"10.1007/s11274-024-04188-4","DOIUrl":"10.1007/s11274-024-04188-4","url":null,"abstract":"Microbial amylases should essentially remain active at higher temperatures, and in the alkaline pH and a range of surfactants to be suitable as detergent additives. In the present study, a thermophilic amylase producing bacterium, Bacillus licheniformis UDS-5 was isolated from Unai hot water spring in Gujarat, India. It was identified as a potent amylase producer during starch plate-based screening process. Therefore, the physicochemical parameters influencing amylase production were optimized using Plackett-Burman design and Central Composite Design. The amylase was purified through ammonium sulfate precipitation, size exclusion and ion exchange chromatography, achieving the purification fold and yield to be 9.2 and 40.6%, respectively. The enzyme displayed robust stability and activity across a wide range of temperatures and pHs, with an increased half-life and reduced deactivation rate constant. The amylase exhibited optimal catalysis at 70 °C and pH 8. The kinetic studies revealed Km and Vmax values of 0.58 mg/mL and 2528 μmol/mL/min, respectively. Besides, the purified amylase displayed stability in the presence of various metal ions, surfactants, and chelators suggesting its potential for industrial applications, particularly in the detergent industry. Moreover, detergent application studies demonstrated its efficacy in enhancing washing performance. A comparative profile on washing efficiency of the studied amylase and the commercial amylase with various detergents pointed towards its possible future use as a detergent additive.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"385"},"PeriodicalIF":4.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galactose-1-phosphate uridylyltransferase GalT promotes biofilm formation and enhances UV-B resistance of Bacillus thuringiensis. 半乳糖-1-磷酸尿苷酰转移酶 GalT 可促进苏云金芽孢杆菌生物膜的形成并增强其抗紫外线-B 的能力。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-18 DOI: 10.1007/s11274-024-04195-5

Aisha Lawan Idris, Xiao Fan, Wen Li, Hankun Pei, Musa Hassan Muhammad, Xiong Guan, Tianpei Huang

{"title":"Galactose-1-phosphate uridylyltransferase GalT promotes biofilm formation and enhances UV-B resistance of Bacillus thuringiensis.","authors":"Aisha Lawan Idris, Xiao Fan, Wen Li, Hankun Pei, Musa Hassan Muhammad, Xiong Guan, Tianpei Huang","doi":"10.1007/s11274-024-04195-5","DOIUrl":"https://doi.org/10.1007/s11274-024-04195-5","url":null,"abstract":"Ultraviolet radiation (UV) is a major abiotic stress resulting in relative short duration of Bacillus thuringiensis (Bt) biopesticides in the field, which is expected to be solved by formation of Bt biofilm with higher UV resistance. Therefore, one of the important prerequisite works is to clarify the functions of biofilm-associated genes on biofilm formation and UV resistance of Bt. In this study, comparative genomics and bioinformatic analysis indicated that BTXL6_19475 gene involved in biofilm formation of Bt XL6 was likely to encode a galactose-1-phosphate uridylyltransferase (GalT, E.C. 2.7.7.12). Heterologous expression of the BTXL6_19475 gene in Escherichia coli and detection of its GalT enzyme activity in vitro proved that the gene did encode GalT. Comparing the wild type Bt strain XL6 with galT gene knockout mutant Bt XL6ΔgalT and its complementary strain Bt XL6ΔgalT::19,475, GalT promoted the biofilm formation and enhanced the UV-B resistance of Bt XL6 likely by increasing its D-ribose production and reducing its alanine aryldamidase activity. GalT did not affect the growth and the cell motility of Bt XL6. A regulation map had been proposed to elucidate how GalT promoted biofilm formation and enhanced UV-B resistance of Bt XL6 by the cross-talk between Leloir pathway, Embden-Meyerhof glycolysis pathway and pentose phosphate pathway. Our finding provides a theoretical basis for the efficient use of biofilm genes to improve the UV resistance of Bt biofilms and thus extend field duration of Bt formulations based on biofilm engineering.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"383"},"PeriodicalIF":4.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic and functional profiling of microbial community in municipal solid waste dumpsite. 城市固体废弃物倾倒场微生物群落的分类和功能分析。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-18 DOI: 10.1007/s11274-024-04189-3

Zuriash Mamo, Sewunet Abera, Mesfin Tafesse

{"title":"Taxonomic and functional profiling of microbial community in municipal solid waste dumpsite.","authors":"Zuriash Mamo, Sewunet Abera, Mesfin Tafesse","doi":"10.1007/s11274-024-04189-3","DOIUrl":"https://doi.org/10.1007/s11274-024-04189-3","url":null,"abstract":"Understanding the microbial ecology of landfills is crucial for improving waste management strategies and utilizing the potential of these microbial communities for biotechnological applications. This study aimed to conduct a comprehensive taxonomic and functional profiling of the microbial community present in the Addis Ababa municipal solid waste dumpsite using a shotgun metagenomics sequencing approach. The taxonomic analysis of the sample revealed the significant presence of bacteria, with the Actinomycetota (56%), Pseudomonadota (23%), Bacillota (3%), and Chloroflexota (3%) phyla being particularly abundant. The most abundant KEGG categories were carbohydrates metabolism, membrane transport, signal transduction, and amino acid metabolism. The biodegradation and metabolism of xenobiotics, as well as terpenoids and polyketides, were also prevalent. Moreover, the Comprehensive Antibiotic Resistance Database (CARD) identified 52 antibiotic resistance gene (ARG) subtypes belonging to 14 different drug classes, with the highest abundances observed for glycopeptide, phosphonic acid, and multidrug resistance genes. Actinomycetota was the dominant phylum harboring ARGs, followed by Pseudomonadota and Chloroflexota. This study offers valuable insights into the taxonomic and functional diversity of the microbial community in the Addis Ababa municipal solid waste dumpsite. It sheds light on the widespread presence of metabolically versatile microbes, antibiotic resistance genes, mobile genetic elements, and pathogenic bacteria. This understanding can contribute to the creation of efficient waste management strategies and the investigation of possible biotechnological uses for these microbial communities.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"384"},"PeriodicalIF":4.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Identification and biochemical characterization of a novel halolysin from Halorubellus sp. PRR65 with a relatively high temperature activity. 更正：具有相对较高温度活性的 Halorubellus sp.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-14 DOI: 10.1007/s11274-024-04196-4

Yuling Hao, Yu Jin, Aodi Zhang, Xinran Jiang, Ming Gong, Cunlong Lu, Ruru Pan, Shaoxing Chen

引用次数: 0

Cyanobacteria's power trio: auxin, siderophores, and nitrogen fixation to foster thriving agriculture. 蓝藻的动力三重奏：辅助素、苷元和固氮，促进农业蓬勃发展。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-13 DOI: 10.1007/s11274-024-04191-9

Adriana Sturion Lorenzi, Mathias Ahii Chia

{"title":"Cyanobacteria's power trio: auxin, siderophores, and nitrogen fixation to foster thriving agriculture.","authors":"Adriana Sturion Lorenzi, Mathias Ahii Chia","doi":"10.1007/s11274-024-04191-9","DOIUrl":"10.1007/s11274-024-04191-9","url":null,"abstract":"Cyanobacteria, often overlooked in traditional agriculture, are gaining recognition for their roles in enhancing plant growth and soil health through diverse mechanisms. This review examines their multifaceted contributions to agricultural systems, highlighting their proficiency in auxin production, which promotes plant growth and development. Additionally, we examined cyanobacteria's ability to produce siderophores that enhance iron absorption and address micronutrient deficiencies, as well as their capacity for nitrogen fixation, which converts atmospheric nitrogen into a form that plants can utilize, all with the goal of reducing reliance on synthetic fertilizers. A meta-analysis of existing studies indicates significant positive effects of cyanobacteria on crop yield, although variability exists. While some research shows considerable yield increases, other studies report non-significant changes, suggesting benefits may depend on specific conditions and crop types. The overall random-effects model estimate indicates a significant aggregate effect, with a few exceptions, emphasizing the need for further research to optimize the use of cyanobacteria as biofertilizers. Although cyanobacteria-based products are limited in comparison to seaweed-derived alternatives, for instance, ongoing challenges include regulatory issues and production costs. Integrating cultivation with wastewater treatment could enhance competitiveness and viability in the agricultural market.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"381"},"PeriodicalIF":4.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presence of lactic acid bacteria in hydrogen production by dark fermentation: competition or synergy. 暗发酵制氢过程中乳酸菌的存在：竞争还是协同。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-13 DOI: 10.1007/s11274-024-04167-9

Cindy Correa-Villa, Edilson Moreno-Cárdenas, Johannes de Bruijn

{"title":"Presence of lactic acid bacteria in hydrogen production by dark fermentation: competition or synergy.","authors":"Cindy Correa-Villa, Edilson Moreno-Cárdenas, Johannes de Bruijn","doi":"10.1007/s11274-024-04167-9","DOIUrl":"https://doi.org/10.1007/s11274-024-04167-9","url":null,"abstract":"Dark fermentation in mixed cultures has been extensively studied due to its great potential for sustainable hydrogen production from organic wastes. However, microbial composition, substrate competition, and inhibition by fermentation products can affect hydrogen yield and production rates. Lactic acid bacteria have been identified as the key organisms in this process. On one hand, lactic acid bacteria can efficiently compete for carbohydrate rich substrates, producing lactic acid and secreting bacteriocins that inhibit the growth of hydrogen-producing bacteria, thereby decreasing hydrogen production. On the other hand, due to their metabolic capacity and synergistic interactions with certain hydrogen-producing bacteria, they contribute positively in several ways, for example by providing lactic acid as a substrate for hydrogen generation. Analyzing different perspectives about the role of lactic acid bacteria in hydrogen production by dark fermentation, a literature review was done on this topic. This review article shows a comprehensive view to understand better the role of these bacteria and their influence on the process efficiency, either as competitors or as contributors to hydrogen production by dark fermentation.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"380"},"PeriodicalIF":4.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of an aminotransferase TlBCAT from Trichoderma longibrachiatum UN32 involved in dendrobine-type total alkaloids biosynthesis. 参与石斛碱类总生物碱生物合成的长叶毛霉 UN32 的氨基转移酶 TlBCAT 的特征。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-09 DOI: 10.1007/s11274-024-04187-5

Xu Qian, Tuifan Yu, Yiwen Cao, Yuanyuan Dong, Surendra Sarsaiya, Jishuang Chen

{"title":"Characterization of an aminotransferase TlBCAT from Trichoderma longibrachiatum UN32 involved in dendrobine-type total alkaloids biosynthesis.","authors":"Xu Qian, Tuifan Yu, Yiwen Cao, Yuanyuan Dong, Surendra Sarsaiya, Jishuang Chen","doi":"10.1007/s11274-024-04187-5","DOIUrl":"https://doi.org/10.1007/s11274-024-04187-5","url":null,"abstract":"Trichoderma longibrachiatum UN32 is an industrially important fungus capable of producing Dendrobine-Type Total alkaloids (DTTAs). Several reports have pointed out that branched-chain amino acid aminotransferases (BCATs) participate in backbone modification or promote the production of secondary metabolites. We previously proposed that cobalt chloride increased DTTAs production in T. longibrachiatum UN32, which was associated with enhanced expression of the gene BCAT, TlBCAT (Genbank accession No. PP465542). Following cloning and characterization, the cDNA of TlBCAT was found to consists of 1191 bp, encoding a protein of 397 amino acid residues. The molecular mass of TlBCAT was about 42 kDa through SDS-PAGE analysis. The predicted pI value was 5.54. Recombinant TlBCAT can catalyze L-leu with a catalytic efficiency of 15.91 mM- 1S- 1. In the binding pocket, residues interacting with PLP, including Tyr68, Arg93, Tyr136, and Lys194, are highly conserved. TlBCAT exhibits a broad spectrum of substrate specificity, typical for catalyzing the transamination reaction of various branched-chain and hydrophobic amino acids, with α-ketoglutarate as the amino acceptor. Exogenous branched-chain amino acids (BCAAs) positively affect Trichoderma growth and DTTAs production. These findings offer insights into the physiological significance of BCAAs and present a novel approach for enhancing DTTAs production in Trichoderma mycelium cultures.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"379"},"PeriodicalIF":4.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction, characterization and antioxidative potentials of UV-screening compound, mycosporine-like amino acids from epilithic cyanobacterium Lyngbya sp. HKAR - 15. 从附生蓝藻 Lyngbya sp. HKAR - 15 中提取紫外线屏蔽化合物、类霉菌素氨基酸及其特征和抗氧化潜力。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-11-06 DOI: 10.1007/s11274-024-04184-8

Abha Pandey, Nasreen Amin, Vinod K Kannaujiya, Rajeshwar P Sinha

{"title":"Extraction, characterization and antioxidative potentials of UV-screening compound, mycosporine-like amino acids from epilithic cyanobacterium Lyngbya sp. HKAR - 15.","authors":"Abha Pandey, Nasreen Amin, Vinod K Kannaujiya, Rajeshwar P Sinha","doi":"10.1007/s11274-024-04184-8","DOIUrl":"https://doi.org/10.1007/s11274-024-04184-8","url":null,"abstract":"Mycosporine-like amino acids (MAAs) are a unique class of UV-screening bioactive molecules with potent antioxidants and photoprotective properties, synthesized by various species of cyanobacteria in different habitats. The cyanobacterial biofilms play a crucial driver in the development of ecological communities. The current study examined the existence of the photoprotective MAAs in a novel epilithic cyanobacterium Lyngbya sp. strain HKAR-15 isolated from cyanobacterial biofilms on the rock surface. The isolated MAAs were identified, purified and characterized using UV-Vis spectroscopy, HPLC (High-Performance Liquid Chromatography), ESI-MS (Electrospray Ionization-Mass Spectrometry), FTIR (Fourier Transform Infrared Spectroscopy) and NMR (Nuclear Magnetic Resonance). The compounds were recognized as palythine (retention time (RT): 2.7 min; UV λmax: 320 nm; m/z: 245.02) and porphyra-334 (RT: 3.6 min; UV λmax: 334 nm; m/z: 347.1). FTIR spectroscopy analyses also revealed the presence of functional groups of both compounds. NMR spectroscopy analyses confirmed the presence of both palythine and porphyra-334. The UV-induced production of both MAAs was visualized under ultraviolet radiation (UVR) in contrast to the photosynthetically active radiation (PAR). The MAAs (palythine and porphyra-334) had a significant dose-dependent free radical scavenging capacity. The findings show that MAAs perform a dynamic role in the survival and photoprotection of cyanobacteria in hostile environments under high solar UV irradiances. These photoprotective compounds may have various biotechnological applications as well as role in the development of natural sunscreens.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"378"},"PeriodicalIF":4.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0