World journal of microbiology & biotechnology最新文献

{"title":"Simultaneous removal of heavy metals and inorganic nitrogen by using the biofilm of Marichromatium gracile YL28.","authors":"Liang Cui, Shicheng Chen, Xiaxing Cao, Xiaobo Zhang, Xiaoping Huang, Tomoyuki Shibata, Yi Yang, Luyao Shi, Chungui Zhao, Shasha Wang, Suping Yang","doi":"10.1007/s11274-024-04193-7","DOIUrl":"https://doi.org/10.1007/s11274-024-04193-7","url":null,"abstract":"<p><p>Heavy metal and nitrogen contaminations are serious concerns in aquatic environments. Marichromatium gracile YL28, a marine purple sulfur bacterium, has shown great potential as a bioremediation agent for removing inorganic nitrogen from marine water. This study further investigated its ability to simultaneously absorb heavy metals, including Pb(II), Cu(II), Cd(II) and Cr(VI), and remove inorganic nitrogen. The contributions of photopigment and extracellular polymeric substances (EPS) in the YL28 biofilm to heavy metal adsorption and tolerance were also evaluated. The YL28 biofilm demonstrated higher adsorption efficiencies for heavy metal ions than planktonic cells. A high level of EPS was detected in the biofilm. The effects of four heavy metal on the inhibition of photopigment synthesis showed that high concentrations of Cu(II) greatly inhibited the production of BChl a and Car. The adsorption efficiencies of Pb(II), Cu(II), Cd(II), and Cr(VI) in the YL28 biofilm reactor reached 86.59%, 72.94%, 80.06%, and 95.95%, respectively. Elevated concentrations of heavy metal ions only marginally impeded ammonia nitrogen removal; they impacted neither nitrite and nitrate removals nor hindered the simultaneous elimination of three inorganic nitrogen compounds. Coupled with their ability to remove inorganic nitrogen, the high adsorption capacity and tolerance of YL28 biofilms toward heavy metal suggest a promising solution for mitigating metal pollutants.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"14"},"PeriodicalIF":4.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic interaction of antibiotic resistance between plant microbiome and organic fertilizers: sources, dissemination, and health risks.","authors":"Habib Ullah, Sedky H A Hassan, Qi Yang, El-Sayed Salama, Pu Liu, Xiangkai Li","doi":"10.1007/s11274-024-04214-5","DOIUrl":"10.1007/s11274-024-04214-5","url":null,"abstract":"<p><p>Antibiotic resistance is a global health problem driven by the irrational use of antibiotics in different areas (such as agriculture, animal farming, and human healthcare). Sub-lethal concentrations of antibiotic residues impose selective pressure on environmental, plant-associated, and human microbiome leading to the emergence of antibiotic-resistant bacteria (ARB). This review summarizes all sources of antibiotic resistance in agricultural soils (including manure, sewage sludge, wastewater, hospitals/pharmaceutical industry, and bioinoculants). The factors (such as the physicochemical properties of soil, root exudates, concentration of antibiotic exposure, and heavy metals) that facilitate the transmission of resistance in plant microbiomes are discussed. Potential solutions for effective measures and control of antibiotic resistance in the environment are also hypothesized. Manure exhibits the highest antibiotics load, followed by hospital and municipal WW. Chlortetracycline, tetracycline, and sulfadiazine have the highest concentrations in the manure. Antibiotic resistance from organic fertilizers is transmitted to the plant microbiome via horizontal gene transfer (HGT). Plant microbiomes serve as transmission routes of ARB and ARGS to humans. The ingestion of ARB leads to human health risks (such as ineffectiveness of medication, increased morbidity, and mortality).</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"4"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the antidiabetic activity of potential probiotic bacteria isolated from traditional fermented beverage.","authors":"Bishwapriya Chutia, Partha P Dutta, Lunasmrita Saikia, Purvita Chowdhury, Munmi Borah, Dharmeswar Barhoi, Rupesh Kumar, Siddhartha N Borah, Debajit Borah, Ajay Kumar Manhar, Manabendra Mandal, Bhaskarjyoti Gogoi","doi":"10.1007/s11274-024-04226-1","DOIUrl":"https://doi.org/10.1007/s11274-024-04226-1","url":null,"abstract":"<p><p>Type 2 Diabetes continues to be one of the major public health issues worldwide without any sustainable cure. The modulation of gut microbiota is believed to be caused by probiotic bacteria and several probiotic strains have previously shown antidiabetic activity. The present study aims to isolate potential probiotic bacteria from traditionally used fermented rice beer of Assam, India and to investigate its anti-hyperglycemic effect. Of the 20 isolated bacterial isolates, 5 isolates showed potential probiotic activities, of which, 2 isolates viz. Bacillus sp. FRB_A(A) and Acetobacter sp. FRB_B(S) showed good in vitro anti-oxidant and anti α-glucosidase activities. Based on the in vitro results, isolate Bacillus sp. FRB_A(A) was further used to evaluate the antidiabetic activity in streptozotocin induced diabetic rat model. After 21 days, the blood glucose level in diabetic rats with probiotic administration significantly lowered from 458.00 ± 46.62 mg/dl to 108.20 ± 6.76 mg/dl (p < 0.001), whereas, in diabetic rats without probiotic remained high (576.20 ± 29.48 mg/dl). On analyzing the endogenous antioxidant profile in various tissues of the experimental rats, reduced lipid peroxidation, glutathione level and superoxide dismutase and glutathione peroxidase activity were observed in probiotic administered rats in comparison to the streptozotocin treated diabetic controls. In conclusion, the bacteria Bacillus sp. FRB_A(A) isolated from fermented rice beer possesses probiotic attributes and exhibits significant anti-hyperglycemic activities.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"10"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and biochemical determinants in potentially toxic metals resistance and plant growth promotion in Rhizobium sp LBMP-C04.","authors":"Daiane Silva Bonaldi, Michelli Inácio Gonçalves Funnicelli, Camila Cesário Fernandes, Henrique Fontellas Laurito, Daniel Guariz Pinheiro, Lucia Maria Carareto Alves","doi":"10.1007/s11274-024-04219-0","DOIUrl":"https://doi.org/10.1007/s11274-024-04219-0","url":null,"abstract":"<p><p>The association of bacteria resistant to potentially toxic metals (PTMs) with plants to remove, transfer, or stabilize these elements from the soil is an appropriate tool for phytoremediation processes in metal-contaminated environments. The objective of this study was to evaluate the potential of Rhizobium sp. LBMP-C04 for phytoremediation processes and plant growth promotion in metal-contaminated soils. Functional annotation allowed us to predict a variety of genes related to PTMs resistance and plant growth promotion in the bacterial genome. Resistance genes are mainly associated with DNA repair, and the import or export of metals in bacterial cells to maintain cell homeostasis. Genes that promote plant growth are related to mechanisms of osmotic stress tolerance, phosphate solubilization, nitrogen metabolism, biological nitrogen fixation, biofilm formation, heat shock responses, indole-3-acetic acid (IAA) biosynthesis, tryptophan, and organic acids metabolism. Biochemical tests indicated that Rhizobium sp. LBMP-C04 can solubilize calcium phosphate and produce siderophores and IAA in vitro in the presence of the PTMs Cd²⁺,Cu²⁺,Cr³⁺,Cr⁶⁺, Zn²⁺, and Ni²⁺. Results indicate the possibility of using Rhizobium sp. LBMP-C04 as a potentially efficient bacterium in phytoremediation processesin environments contaminated by PTMs and simultaneously promote plant growth.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"7"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae bioprospecting for the food industry: insights into the autotrophic biomass production and macromolecular accumulation of four microalgal species.","authors":"Georgia Papapanagiotou, Christina Samara, Paraskevi Psachoulia, Christos Chatzidoukas","doi":"10.1007/s11274-024-04229-y","DOIUrl":"https://doi.org/10.1007/s11274-024-04229-y","url":null,"abstract":"<p><p>In this study, four microalgal strains were evaluated for their biomass production capacity and macromolecule biosynthesis. These include three strains from the phylum Chlorophyta: Monoraphidium sp. LB2PC 0120, Stichococcus sp. LB2PC 0117, and Tetraselmis sp. LB2PC 0320, and one strain from the phylum Haptophyta: Isochrysis sp. LB2PC 0220. The experiments were conducted under typical laboratory-scale setups. Additionally, phylogenetic analysis based on the 18-28 S rRNA internal transcribed spacer (ITS) was performed to validate the taxonomic identity of the strains. Each strain was exposed to four different cultivation conditions based on two levels of illumination intensity [25-(LI) and 50-(HI) µmol m^- 2 s^- 1] and nitrogen loading [100-(LΝ) and 300-(HΝ) mg NaNO₃ L^- 1] in a full factorial design. All the microalgae achieved maximum biomass production under HI-HN conditions, which amounted to 1495, 919, 844, and 708 mg/L for Monoraphidium sp. LB2PC 0120, Stichococcus sp. LB2PC 0117, Tetraselmis sp. LB2PC 0320 and Isochrysis sp. LB2PC 0220, respectively, after 16 days of cultivation. Among them, Stichococcus sp. LB2PC 0117 had the highest protein content (49.9% wt.) under LI-HN conditions and Monoraphidium sp. LB2PC 0120 had the highest lipid content (44.3% wt.) under HI-LN conditions. Both Monoraphidium sp. LB2PC 0120 and Tetraselmis sp. LB2PC 0320 accumulated the highest carbohydrate content (~ 37% wt.) under LI-LN and HI-LN conditions, respectively. Based on biomass and macromolecule production, Monoraphidium sp. LB2PC 0120 was identified as the most promising candidate for upscaling studies, expecting its highly manipulatable compositional profile to support multiple applications in the food industry, rendering this microalga a valuable resource.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"12"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thriving in salty environments: Aspergillus niger's halotolerance and BTEX biodegradation potential.","authors":"Kelly Rodrigues, Ricardo Salgado, Dina Galhanas, Victoria Maura S Bermudez, Glória Maria Marinho Silva, Ana Maria Álvares Tavares da Mata, Luciana Pereira","doi":"10.1007/s11274-024-04220-7","DOIUrl":"https://doi.org/10.1007/s11274-024-04220-7","url":null,"abstract":"<p><p>Benzene, toluene, ethylbenzene, and xylene (BTEX) can be found in marine and estuarine waters due to accidental spills of oil and derivatives, as well as in production water and effluents discharged from petrochemical plants. Addressing the bioremediation of these compounds in saline environments and effluents with elevated salinity levels is imperative. In this study, the halotolerance of Aspergillus niger was assessed by subjecting it to a stepwise increase in salinity, achieved through progressive addition of NaCl from 2 to 30‰ (v/v). The fungal strain exhibited optimal growth support up to a salinity concentration of 25‰, accompanied by a biomass production rate of (0.93 ± 0.11) g.d^-1. The adapted biomass was employed in batch reactors to evaluate the biodegradation of BTEX (1,500 mg.L^-1). In the absence of sucrose, the reactors inoculated with fungi demonstrated almost complete BTEX removal within 7 days, with rates ranked as follows: benzene (1.12 d^-1) > toluene (0.78 d^-1) > ethylbenzene (0.65 d^-1) > xylene (0.63 d^-1). Enhanced BTEX removal rates were obtained in the presence of sucrose, notably with 2 g.L^-1: benzene (3.63 d^-1) > toluene (2.10 d^-1) > ethylbenzene (1.56 d^-1) > xylene (1.50 d^-1). Notably, benzene was found to be the sole compound adsorbed onto the fungal mycelium (1.50 ± 0.19) to (13.35 ± 4.72) mg.g^-1 of biomass.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"6"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent findings on metabolomics and the microbiome of oral bacteria involved in dental caries and periodontal disease.","authors":"Sachio Tsuchida, Hiroshi Umemura, Kazuhide Iizuka, Haruka Yamamoto, Isamu Shimazaki, Elisa Shikata, Tomohiro Nakayama","doi":"10.1007/s11274-024-04224-3","DOIUrl":"10.1007/s11274-024-04224-3","url":null,"abstract":"<p><p>Periodontal disease is characterized by bacterial toxins within the oral biofilm surrounding the teeth, leading to gingivitis and the gradual dissolution of the alveolar bone, which supports the teeth. Notably, symptoms in the early stages of the disease are often absent. Similarly, dental caries occurs when oral bacteria metabolize dietary sugars, producing acids that dissolve tooth enamel and dentin. These bacteria are commonly present in the oral cavity of most individuals. Metabolomics, a relatively recent addition to the \"omics\" research landscape, involves the comprehensive analysis of metabolites in vivo to elucidate pathological mechanisms and accelerate drug discovery. Meanwhile, the term \"microbiome\" refers to the collection of microorganisms within a specific environmental niche or their collective genomes. The human microbiome plays a critical role in health and disease, influencing a wide array of physiological and pathological processes. Recent advances in microbiome research have identified numerous bacteria implicated in dental caries and periodontal disease. Additionally, studies have uncovered various pathogenic factors associated with these microorganisms. This review focuses on recent findings in metabolomics and the microbiome, specifically targeting oral bacteria linked to dental caries and periodontal disease. We acknowledge the limitation of relying exclusively on the MEDLINE database via PubMed, while excluding other sources such as gray literature, conference proceedings, and clinical practice guidelines.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"11"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying multidrug-resistant organisms in diabetic foot ulcers: a study of risk factors and antimicrobial resistance genes.","authors":"Mohd Saleem, Soha Abdallah Moursi, Tahani Nasser Almofeed Altamimi, Alharbi Mohammed Salem, Alwaleed Mohammad Alaskar, Sahar Adly Hassan Hammam, Ehab Rakha, Syed Muhammad Ozair Ilyas, Hamoud Abdulmohsin Al-Malaq, Metab Nasser Alshammari, Azharuddin Sajid Syed Khaja","doi":"10.1007/s11274-024-04209-2","DOIUrl":"https://doi.org/10.1007/s11274-024-04209-2","url":null,"abstract":"<p><p>This study aims to evaluate the antibiotic susceptibility profiles of bacterial isolates from DFU patients, identify the prevalence of MDROs, and identify specific risk factors contributing to these infections to inform effective antibiotic treatment strategies. This prospective cohort study included 187 DFU patients from March 2023 to February 2024 at King Khalid Hospital, Saudi Arabia. The exclusion criteria were nondiabetic ulcers, specific infections, tumours, or recent antibiotic use. Data on demographics, diabetes duration, DFU characteristics, and antibiotic history were collected. Ethical approval and informed consent were obtained. During the 1-year study, 187 DFU patients were included, with 72.7% males and 27.3% females. The mean age of the study participants was 54.9 ± 11.8 years. The average duration of diabetes was 6.3 ± 3.7 years, and the mean HbA1c was 8.0 ± 1.0%. Over half (54.5%) of the patients had Wagner ulcer grade III, and neuropathy (67%) and retinopathy (73%) were the most common complications. Polymicrobial infections were identified in 54% of the cases, with 61.5% of the isolates producing biofilms. The key risk factors for MDR infections included amputation (OR: 5.92), polymicrobial infections (OR: 7.49), biofilm production (OR: 5.00), recent antibiotic use (OR: 3.97), and an ulcer duration > 30 days (OR: 2.23). Sex, age, and weight were not significantly associated with MDR infections. Among the 27 MRSA isolates, 81.5% carried the mecA gene, and 98.8% of the ESBL-producing organisms harboured at least one ESBL gene, with blaCTX-M being the most common (27.2%). Carbapenem resistance was confirmed in 25.6% of the isolates, with blaNDM being the predominant carbapenemase gene (69.3%). Coresistance with ESBL genes was detected in 66.7% of the blaNDM-producing isolates. This study demonstrates a high prevalence of MDROs in DFUs, primarily driven by biofilm-producing polymicrobial infections and resistance genes like blaNDM and blaCTX-M. The findings accentuate the clinical importance of integrating molecular diagnostics for early detection of resistance determinants, enabling precise, targeted therapy. Tailored antibiotic stewardship and enhanced infection control measures are critical to optimizing treatment outcomes, reducing complications, and mitigating the burden of chronic DFUs in healthcare settings.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"3"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New complete genome insights into Enterobacter roggenkampii FACU2: a potential player in cadmium bio-removal.","authors":"Asmaa A Halema, Mostafa A Abdel-Maksoud, Mohamed Y Ali, Abdul Malik, Bushra Hafeez Kiani, Ahmed R Henawy, Nagwa I Elarabi, Abdelhadi A Abdelhadi, Heba A R Abdelhaleem","doi":"10.1007/s11274-024-04138-0","DOIUrl":"https://doi.org/10.1007/s11274-024-04138-0","url":null,"abstract":"<p><p>Industrial workplaces, particularly those involved in ore processing or smelting, pose a high risk of exposure to cadmium, a highly toxic metal. This study isolated and identified eight cadmium-resistant strains from industrial wastewater for their ability to resist cadmium. Enterobacter roggenkampii FACU2 demonstrated exceptional cadmium removal capabilities during our analysis, successfully eliminating 62% of the cadmium. Additionally, transmission electron microscopy (TEM) was utilized to examine the morphological change between the most and least efficient strains that responded to cadmium stress at the cellular level. Compared to the control bacteria, the treated bacteria exhibited notably higher levels of cadmium adsorption and accumulation within their cells. A complete genome analysis revealed that E. roggenkampii FACU2 has one chromosome and one plasmid with a size of 4,856,454 bp and 80,926 bp, respectively, in addition to harboring numerous heavy metal-resistant genes related to cadmium and other heavy metals. Moreover, the gene expression of four cadmium-resistant genes (czcA, cadA, czcC and czcD) showed that the high cadmium concentration led to a significant increase in czcA and cadA mRNA levels, thus indicating the activation of cadmium-resistant genes in the E. roggenkampii FACU2 compared to Enterobacter sp. strain FACU. Due to its ability to remove cadmium and other heavy metals, this strain holds promise as a source of genes for biological treatment methods. This application could contribute to environmental purification, ultimately benefiting human health.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"2"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatile organic compounds from Stenotrophomonas geniculata J-0 as potential biofumigants manage bulb rot caused by Fusarium oxysporum in postharvest Lanzhou lily.","authors":"Lijun Ling, Rui Yue, Yuanyuan Wang, Lijun Feng, Ling Yang, Yao Li, Rongxiu Mo, Wenyue Zhang, Fanjin Kong, Yijuan Jiang, Yongpeng Zhou","doi":"10.1007/s11274-024-04228-z","DOIUrl":"https://doi.org/10.1007/s11274-024-04228-z","url":null,"abstract":"<p><p>The Lanzhou lily bulbs are often vulnerable to postharvest infections by pathogenic fungi, leading to lily bulb rot. This study investigated the ability of volatile organic compounds (VOCs) produced by Stenotrophomonas geniculata J-0 to control the highly pathogenic fungus Fusarium oxysporum BH-7 in postharvest Lanzhou lily bulbs. VOCs of S. geniculata J-0 showed inhibitory effect on the mycelial growth of F. oxysporum BH-7, with a maximum inhibition of 100%. Scanning electron microscope (SEM) observed that VOCs caused a shift in mycelial morphology from elongated and uniform tubular to collapsed and wrinkled. Moreover, VOCs of J-0 significantly reduced pathogenic fungal spore germination and sporulation. Through headspace gas chromatography-ion mobility spectrometry analysis, J-0 emitted 15 volatile compounds. The fumigation test of BH-7 with single pure synthetic compounds showed that 1-penten-3-one had excellent antifungal activity, with an inhibition rate of 100% at 4 μL/L. Additionally, our results revealed 1-penten-3-one destroyed the integrity and increased the permeability of BH-7 mycelial cell membranes, leading to leakage of intracellular electrolytes and substances, a reduction in extracellular pH, a blockage of ergosterol synthesis and an elevation in malondialdehyde content. In vivo experiments, fumigation of 1-penten-3-one at an exceptionally low concentration (4 μL/L) for a very short period of time (0.5 h) was effective in delaying the onset and prevalence of postharvest diseases. Hence, this study provides novel antifungal agents to control disease in postharvest Lanzhou lily and enhances our understanding of the biocontrol potential of volatiles from S. geniculata.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"9"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}