World journal of microbiology & biotechnology最新文献_第2页

Bacterial outer membrane vesicles: a novel target in mediating bacterial infection and host immune responses. 细菌外膜囊泡：介导细菌感染和宿主免疫反应的新靶点。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-08 DOI: 10.1007/s11274-025-04597-z

Beiliang Miao, Jiatong Han, Yuru Jiang, Li Yu, Yingjun Liu, Fangyuan Du, Xinyu Zhu, Xuezhong Gong, Zhengsheng Li, Zeliang Chen, Shiwei Liu

{"title":"Bacterial outer membrane vesicles: a novel target in mediating bacterial infection and host immune responses.","authors":"Beiliang Miao, Jiatong Han, Yuru Jiang, Li Yu, Yingjun Liu, Fangyuan Du, Xinyu Zhu, Xuezhong Gong, Zhengsheng Li, Zeliang Chen, Shiwei Liu","doi":"10.1007/s11274-025-04597-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04597-z","url":null,"abstract":"Outer membrane vesicles (OMVs), originating from the outermost membrane of cells, are the extracellular vesicles released by bacteria, containing bacterial outer membrane components such as phospholipids, lipopolysaccharides (LPS), outer membrane proteins, and bacteria-specific antigens. OMVs have progressively been acknowledged as a novel secretory system, playing a pivotal role in mediating bacterial infections and modulating host immune responses. They are anticipated to emerge as a promising therapeutic target for the intervention of bacterial infectious diseases in the future. Based on this, the present article provides a comprehensive review of the role of OMVs in bacterial infections and immune modulation. This article summarizes that OMVs can mediate bacterial infections by transporting virulence genes and antibiotic resistance genes, as well as by carrying pathogen-associated molecular patterns (PAMPs). Additionally, they modulate host immune functions through direct interactions with immune cells such as neutrophils, macrophages, and dendritic cells. Furthermore, OMVs can facilitate bacterial immune evasion by modulating biofilm formation, resisting host antimicrobial peptides, and inhibiting the functionality of the complement system. Given that OMVs are non-replicative and contain a wealth of bacterial antigens, coupled with their ability to effectively activate the immune system, they are regarded as highly promising vaccine candidates. This article concludes by summarizing the concepts and strategies for developing vaccines based on OMVs. It is anticipated that this article will provide a more comprehensive understanding of the role of OMVs in research related to bacterial infectious diseases.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"363"},"PeriodicalIF":4.2,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioplastic production in air-lift bioreactors using a spent cooking oil-based method for sustainable recovery. 气升式生物反应器中生物塑料的生产，采用废油为基础的可持续回收方法。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-08 DOI: 10.1007/s11274-025-04606-1

Ivana Antolin, Lara Sanchez Rizza, Julieta Agustina Crisci, Leonardo Curatti

{"title":"Bioplastic production in air-lift bioreactors using a spent cooking oil-based method for sustainable recovery.","authors":"Ivana Antolin, Lara Sanchez Rizza, Julieta Agustina Crisci, Leonardo Curatti","doi":"10.1007/s11274-025-04606-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04606-1","url":null,"abstract":"Global concern over plastic pollution and fossil resource depletion has driven the development of biodegradable alternatives like polyhydroxybutyrate (PHB). PHB, synthesized by microorganisms, faces commercialization challenges due to high production and separation costs. This study evaluated an alternative platform for sustainable PHB production using air-lift bioreactors (ALRs) of up to 15 L. We achieved a high concentration, enrichment and specific productivity of PHB of 4.4 g ± 0.1 g·L⁻¹, 87.9 ± 0.3% (w/w) and 0.43 ± 0.0 g·L⁻¹·h-1, in an Azotobacter vinelandii mutant strain deficient in alginate biosynthesis, in 28 h. Bacterial cells were cultured using air as the sole N source. PHB was recovered with 96% efficiency and 95% purity by osmotic shock using glycerol and soap produced from recycled spent cooking oil. This strategy not only replaces commonly used hazardous solvents for PHB extraction but also repurposes spent cooking oil, an otherwise difficult-to-manage waste, into a valuable resource for bioplastics production. Additionally, we demonstrate the feasibility of recycling glycerol recovered after PHB extraction, which partially substituted for sucrose as a carbon source in subsequent PHB production. The cells exhibited a preferential utilization of C sources in the following order: sucrose > analytical-grade glycerol > glycerol recovered from recycled spent cooking oil post-osmotic shock and PHB recovery > fresh glycerol recovered from recycled spent cooking oil. These findings provide practical opportunities for improving the environmental and economic sustainability of PHB production while aligning with circular economy objectives, supporting the gradual replacement of certain petrochemical plastics.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"364"},"PeriodicalIF":4.2,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing stability of enzymes for industrial applications: molecular insights and emerging approaches. 提高酶的稳定性用于工业应用：分子的见解和新兴的方法。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-08 DOI: 10.1007/s11274-025-04568-4

Mohd Faheem Khan

{"title":"Enhancing stability of enzymes for industrial applications: molecular insights and emerging approaches.","authors":"Mohd Faheem Khan","doi":"10.1007/s11274-025-04568-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04568-4","url":null,"abstract":"The growing demand for robust biocatalysts in industrial bioprocesses has intensified the pursuit of enzymes capable of functioning under extreme physicochemical conditions. This work critically examines the molecular determinants of enzyme stability, including thermostability, pH tolerance, halotolerance, resistance to solvents and oxidative stress, mechanical resilience to shear and pressure, and storage stability. These traits are essential for sustained catalytic performance in sectors such as bioenergy, pharmaceuticals, food, textiles, and environmental remediation. Recent advances in structure-guided engineering, molecular dynamics, and mutational profiling have enabled rational strategies to enhance enzyme resilience. By adopting a multi-parametric lens, this study bridges specific molecular adaptations with industrial challenges, offering a systems-level framework often lacking in single-condition analyses. It also explores emerging frontiers, including AI-assisted enzyme design, metagenomic discovery from extremophiles, and synthetic reconstruction of adaptive pathways, paving the way for next-generation biocatalysts optimised for scalability, performance, and sustainability. The novelty of this work lies in its integrative approach combining molecular insight, environmental origin, and computational tools to guide the development of industrially robust enzymes.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"362"},"PeriodicalIF":4.2,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel degenerate PCR primers targeting the β-galactosidase gene for broad-spectrum identification of lactose-degrading bacteria. 针对β-半乳糖苷酶基因的新型退化PCR引物用于乳糖降解菌的广谱鉴定。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-04 DOI: 10.1007/s11274-025-04601-6

Canting Shen, Qinglong Shu

{"title":"Novel degenerate PCR primers targeting the β-galactosidase gene for broad-spectrum identification of lactose-degrading bacteria.","authors":"Canting Shen, Qinglong Shu","doi":"10.1007/s11274-025-04601-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04601-6","url":null,"abstract":"β-galactosidase (EC 3.2.1.23), a pivotal enzyme in lactose degradation, is widely distributed across numerous bacterial species. This study aimed to develop degenerate primers with high specificity and universality, using the β-galactosidase gene as a molecular marker to identify diverse lactose-degrading bacteria in environmental samples. High-quality β-galactosidase gene sequences belonging to glycoside hydrolase family 2 were retrieved from the NCBI and CAZY databases for sequence alignment and phylogenetic analysis. Through extensive screening and optimization of full-length sequences, a pair of primers was successfully designed for detecting environmental β-galactosidase genes. The newly developed primers were validated using four types of DNA samples, revealing the diversity of lactose-degrading bacterial communities: Human feces (2 phyla, 5 genera), Rat feces (3 phyla, 3 genera), Mouse feces (2 phyla, 6 genera), and soil (6 phyla, 13 genera). By optimizing sequences of representative strains based on the full-length target gene, this study successfully developed novel degenerate primers, providing a reference strategy for addressing the challenge of primer design for highly variable gene families. Additionally, a new molecular probe targeting the β-galactosidase gene was developed to identify diverse lactose-degrading bacteria in environmental samples. To the best of our knowledge, this is the first report of universal primers targeting the β-galactosidase gene for detecting lactose-degrading microbial communities in environmental samples.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"359"},"PeriodicalIF":4.2,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron, manganese, and copper complexes protect saccharomyces cerevisiae from methyl methanesulfonate-induced genotoxicity and oxidative stress. 铁、锰和铜配合物保护酿酒酵母免受甲基磺酸诱导的遗传毒性和氧化应激。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-04 DOI: 10.1007/s11274-025-04595-1

Larissa M M Mattos, Davi A Andrade, Alan N S Alves, Victória T Ferreira, Simone S C Oliveira, Bruna B Segat, André L S Santos, Adolfo Horn, Christiane Fernandes, Marcos D Pereira

{"title":"Iron, manganese, and copper complexes protect saccharomyces cerevisiae from methyl methanesulfonate-induced genotoxicity and oxidative stress.","authors":"Larissa M M Mattos, Davi A Andrade, Alan N S Alves, Victória T Ferreira, Simone S C Oliveira, Bruna B Segat, André L S Santos, Adolfo Horn, Christiane Fernandes, Marcos D Pereira","doi":"10.1007/s11274-025-04595-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04595-1","url":null,"abstract":"Methyl methanesulfonate (MMS) is a well-known classical alkylating agent that induces DNA damage, mutagenesis, and cell dysfunction. Alternatives to mitigate the damage caused by MMS are essential for understanding DNA repair mechanisms and developing approaches to reduce mutagenic and cytotoxic effects of alkylating agents. This study explores the potential of synthetic antioxidants containing iron (1), manganese (2), or copper (3) to protect Saccharomyces cerevisiae from MMS-induced damage. The wild type strain, BY4741, and its isogenic DNA-repair mutants rad9Δ and rad54Δ strains were used to investigate whether these complexes effectively mitigate yeast susceptibility, mitochondrial dysfunction, intracellular oxidation, and mutagenesis caused by MMS. The results demonstrate that all complexes significantly enhanced the survival rates of all fungal strains, indicating their protective role against MMS-induced DNA damage. Additionally, MMS exposure increased mitochondrial dysfunction, intracellular oxidation, and canavanine-based mutagenesis, which was subsequently reduced by the treatment with the complexes, indicating their ability to mitigate oxidative stress and genotoxicity caused by MMS. Among the tested compounds, complex 3 conferred the greatest protective effect against MMS-induced cellular damage, followed by complexes 2 and 1, establishing a consistent order of efficacy: 3 > 2 > 1. These findings demonstrate that the coordination compounds employed in this study effectively protected S. cerevisiae against MMS-induced toxicity, highlighting their potential role in enhancing cellular defense against genotoxic agents.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"358"},"PeriodicalIF":4.2,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and Actinobacteria-based biological suppression of leaf necrosis in tea (Camellia sinensis) caused by Lasiodiplodia theobromae in India. 放线菌对茶树叶片坏死的检测及生物抑制作用。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-03 DOI: 10.1007/s11274-025-04577-3

Shashanka Sonowal, Pranami Bharadwaj, Debajit Thakur

{"title":"Detection and Actinobacteria-based biological suppression of leaf necrosis in tea (Camellia sinensis) caused by Lasiodiplodia theobromae in India.","authors":"Shashanka Sonowal, Pranami Bharadwaj, Debajit Thakur","doi":"10.1007/s11274-025-04577-3","DOIUrl":"https://doi.org/10.1007/s11274-025-04577-3","url":null,"abstract":"Fungal diseases are a common threat to the cultivation of tea [Camellia sinensis (L.)] in India, with Lasiodiplodia theobromae found recently to be a major cause of leaf necrosis. The objective of this work was to isolate and identify fungal pathogens from diseased tea plants and to assess the biocontrol effectiveness of Streptomyces sp. strains OR02 and PTS59 against L. theobromae strain KF16. Four fungal isolates were isolated, among which KF16 was identified as L. theobromae. Antagonistic analysis found that OR02 showed the maximum inhibition zone (24 ± 0.8 mm) and mycelial suppression (91.11%) against KF16, outperforming commercial fungicides, Mancozeb and Hexaconazole. In vitro and In vivo studies revealed that OR02 and PTS59 substantially decreased the formation of necrotic lesions and enhanced plant growth without causing any harmful effects. Improved height, leaf count, and branches were observed in OR02 treated plants, suggesting that it serves as both a biocontrol and growth-promoting agent. The study also revealed that KF16 could systematically infect newly growing leaves, emphasizing its virulence and the pressing need for sustainable control measures. These results highlight Streptomyces sp. OR02 potential as an eco-friendly substitute to chemical fungicides in the treatment of tea diseases, particularly against L. theobromae, and encourages its further development into a bioformulation for field use.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"355"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential characterization of medium-temperature Daqu from different geographical regions: bacterial profile, color, taste, and flavor. 不同地域中温大曲的细菌特征、颜色、味道和风味的差异。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-03 DOI: 10.1007/s11274-025-04560-y

Yurong Wang, Yan Lei, Jianhui Zhang, Qiangchuan Hou, Zhuang Guo

{"title":"Differential characterization of medium-temperature Daqu from different geographical regions: bacterial profile, color, taste, and flavor.","authors":"Yurong Wang, Yan Lei, Jianhui Zhang, Qiangchuan Hou, Zhuang Guo","doi":"10.1007/s11274-025-04560-y","DOIUrl":"https://doi.org/10.1007/s11274-025-04560-y","url":null,"abstract":"In this study, the sensory qualities of MTD from different geographical regions were comprehensively analyzed using electronic sensing technology. Moreover, the culturable Bacillus species, LAB, and other bacterial communities in these MTD samples were identified using culture methods and single-molecule real-time sequencing. The most abundant culturable species of Bacillus and LAB in MTD were Bacillus licheniformis and Pediococcus pentosaceus, respectively. The bacterial diversity was higher in MTD from the Nanyang region than in that from the Luzhou region, and its sensory quality were superior. Nevertheless, Nanyang MTD showed poorer microecological stability. Functional characterization revealed that the bacterial communities present in MTD were actively enriched in processes related to ribosome structure and biogenesis, as well as cell wall/membrane/envelope biogenesis. Notably, Thermoactinomyces daqus and Leuconostoc citreum emerged as the bacterial species with the highest relative abundance in MTD from the Luzhou and Nanyang regions, respectively, and were identified as biomarkers for the MTD from these two regions. Leuconostoc citreum, Leuconostoc mesenteroides, Pediococcus pentosaceus, Thermoactinomyces daqus, and Weissella paramesenteroides was significantly correlated with the synthesis of aromatic compounds (P < 0.05). These findings expand our knowledge of the microbiological composition and quality of MTD from various regions, offering a valuable reference for product optimization.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"354"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for enhancing the adaptive response to oxidative stress in microbial cell factories. 提高微生物细胞工厂对氧化应激适应性反应的策略。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-03 DOI: 10.1007/s11274-025-04544-y

Ziyun Zou, Mei-Li Sun, Kaifeng Wang, Xiao-Jun Ji

{"title":"Strategies for enhancing the adaptive response to oxidative stress in microbial cell factories.","authors":"Ziyun Zou, Mei-Li Sun, Kaifeng Wang, Xiao-Jun Ji","doi":"10.1007/s11274-025-04544-y","DOIUrl":"https://doi.org/10.1007/s11274-025-04544-y","url":null,"abstract":"Reactive oxygen species (ROS), inevitable byproducts of aerobic respiration, exhibit both signaling and cytotoxic effects depending on concentration within cells. At controlled levels, ROS serve as crucial signaling molecules mediating intracellular signal transduction pathways. However, excessive accumulation triggers oxidative stress, leading to oxidative damage that disrupts protein structure and function, ultimately inducing cell death. Enhancing cellular adaptive responses to oxidative stress and mitigating intracellular ROS accumulation are therefore critical strategies for maintaining cellular homeostasis. This approach significantly improves product yield and underpins its widespread application across diverse sectors, including functional foods, pharmaceuticals, and cosmeceuticals. This review first delineates the origins and multifaceted roles of ROS. Subsequently, it details strategies employed to bolster cellular oxidative stress resilience, focusing on process optimization and metabolic engineering approaches. Finally, synthesizing current research advances, existing challenges, and emerging trends, the review outlines future research directions aimed at enhancing the oxidative stress tolerance of microbial cell factories.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"356"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Klebsiella aerogenes PgaB orthologue can efficiently hydrolyze Staphylococcus aureus biofilms. 产气克雷伯菌PgaB同源物能高效水解金黄色葡萄球菌生物膜。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-03 DOI: 10.1007/s11274-025-04550-0

Jéssica Pinheiro Silva, Andrei Nicoli Gebieluca Dabul, Vera Lúcia Mores Rall, Caroline Rosa Silva, Luís Antônio Esmerino, Marcos Pileggi, Felipe Francisco Tuon, Mario de Oliveira Neto, Darlan Nakayama, Alejandra Estela Miranda, Pedro Ricardo Vieira Hamann, Igor Polikarpov

{"title":"Klebsiella aerogenes PgaB orthologue can efficiently hydrolyze Staphylococcus aureus biofilms.","authors":"Jéssica Pinheiro Silva, Andrei Nicoli Gebieluca Dabul, Vera Lúcia Mores Rall, Caroline Rosa Silva, Luís Antônio Esmerino, Marcos Pileggi, Felipe Francisco Tuon, Mario de Oliveira Neto, Darlan Nakayama, Alejandra Estela Miranda, Pedro Ricardo Vieira Hamann, Igor Polikarpov","doi":"10.1007/s11274-025-04550-0","DOIUrl":"https://doi.org/10.1007/s11274-025-04550-0","url":null,"abstract":"Staphylococcus aureus, a gram-positive bacterium, is the prevalent cause of numerous infections. Its ability to form biofilms significantly enhances its pathogenicity, resulting in increased antibiotic resistance and evasion of the host immune response. Poly-β-(1,6)-N-acetylglucosamine (PNAG) plays a crucial role in the formation and maintenance of S. aureus biofilms. In this study, we heterologously expressed KaPgaB from Klebsiella aerogenes and evaluated its efficacy in both degrading and inhibiting S. aureus biofilm formation. Additionally, we investigated the combined effects of KaPgaB with DNase I and papain. Our results demonstrated that KaPgaB alone removed up to 81% of biofilm biomass within 4 h when used at a concentration of 0.5 mg/mL. Moreover, when the enzyme was applied sequentially with DNase I, approximately 97% of adhered biofilms were removed. We also observed significant inhibition of biofilm formation across S. aureus strains. The findings presented in this study might be useful for the development of enzymatic tools capable of degrading S. aureus PNAG-based biofilms.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"353"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Biochemical insight into gut microbial imbalance in Covid-19 and post vaccination heart attacks. 更正：对Covid-19和疫苗接种后心脏病发作的肠道微生物失衡的生化见解。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-10-03 DOI: 10.1007/s11274-025-04566-6

Mohamed Gamil Mehanna, Turky Omar Asar, Habib Changal Mudasir, Sherif Edris, Hadiah Bassam Al Mahdi, Ahmed F Bakry, Mustafa Zeyadi, Mirza Rafi Baig, Salma Naqvi, Fahad A Al-Abbasi, Omar A Al Bar, Vikas Kumar, Firoz Anwar

引用次数: 0