World journal of microbiology & biotechnology最新文献

{"title":"Stress-tolerant Bacillus strains for enhancing tomato growth and biocontrol of Fusarium oxysporum under saline conditions: functional and genomic characterization.","authors":"María F Valencia-Marin, Salvador Chávez-Avila, Edgardo Sepúlveda, Carmen S Delgado-Ramírez, Jenny J Meza-Contreras, Ma Del Carmen Orozco-Mosqueda, Sergio De Los Santos-Villalobos, Olubukola Oluranti Babalola, Rufina Hernández-Martinez, Gustavo Santoyo","doi":"10.1007/s11274-025-04308-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04308-8","url":null,"abstract":"<p><p>Soil salinity is a major limiting factor for agricultural crops, which increases their susceptibility to pathogenic attacks. This is particularly relevant for tomato (Solanum lycopersicum), a salt-sensitive crop. Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici, is a significant threat to tomato production in both greenhouse and field environments. This study evaluated the salinity tolerance, biocontrol, and plant growth-promoting properties of Bacillus velezensis AF12 and Bacillus halotolerans AF23, isolated from soil affected by underground fires and selected for their resistance to saline conditions (up to 1000 mM NaCl). In vitro assays confirmed that both strains produced siderophores, indole-3-acetic acid (IAA), and proteases and exhibited phosphate solubilization under saline stress (100-200 mM NaCl). AF23 exhibited synergistic interactions with AF12, and inoculation with either strain individually or in combination significantly improved the growth of the Bonny Best tomato cultivar under 200 mM saline stress, leading to increased shoot and root weight, enhanced chlorophyll content, and higher total biomass. The biocontrol potential of AF12 and AF23 was evaluated in tomato plants infected with F. oxysporum. Both strains, individually or combined, increased shoot and root weight, chlorophyll content, and total biomass under non-saline conditions, promoting growth and reducing infection rates under saline stress (100 mM NaCl). Genomic analysis revealed that both strains harbored genes related to salt stress tolerance, biocontrol, and plant growth promotion. In conclusion, Bacillus strains AF23 and AF12 demonstrated strong potential as bioinoculants for enhancing tomato growth and providing protection against F. oxysporum in saline-affected soils.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"96"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic microorganisms as an alternative source of thrombolytic compounds: a systematic review.","authors":"Thalya Natasha da Silva Santos, Sara Cadete da Silva, Yanara Alessandra Santana Moura, Marllyn Marques da Silva, Ana Lúcia Figueiredo Porto, Raquel Pedrosa Bezerra","doi":"10.1007/s11274-025-04303-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04303-z","url":null,"abstract":"<p><p>Current conventional thrombolytic drugs have some limitations, including a short half-life, several adverse effects, low fibrin specificity, and high cost. Therefore, new thrombolytic sources have been widely investigated worldwide. In this sense, this work aims to evaluate the state of the art of the thrombolytic potential of different bioactive compounds produced from microalgae and cyanobacteria. Then, a systematic literature search was conducted using ScienceDirect, Medline (PubMed), Springer Link, Wiley Online Library, Scielo, MDPI, and BVS electronic databases to select original studies about thrombolytic agents obtained from microalgae and cyanobacteria. After the selection process, 20 studies met the inclusion criteria and were included in the final analysis. Most studies showed promising thrombolytic activity of polysaccharides or proteins produced by cyanobacteria and obtained through homogenization methods. Moreover, the majority of the studies used methods such as activated partial thromboplastin time, prothrombin time, thrombin time, or platelet aggregation tests as parameters to determine the thrombolytic activity. In conclusion, various bioactive compounds from microalgae and cyanobacteria showed high potential to act as alternative thrombolytic therapy, but some characteristics such as mechanism of action, cytotoxicity, immunogenicity and stability parameters need to be more exploited to make the application of these agents feasible in the future.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"95"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a Na⁺-stimulated acidic hyaluronate lyase from Microbulbifer sp. ALW1 and the antioxidant activity of its hydrolysates.","authors":"Qianli Huang, Chunhua Zhu, Tao Hong, Hebin Li, Lijun Li, Mingjing Zheng, Zhipeng Li, Zedong Jiang, Hui Ni, Yanbing Zhu","doi":"10.1007/s11274-025-04315-9","DOIUrl":"https://doi.org/10.1007/s11274-025-04315-9","url":null,"abstract":"<p><p>Hyaluronic acid (HA) is a natural polymer that can be degraded by hyaluronate lyase into oligomers with diverse biological activities. In this study, a novel hyaluronate lyase (named HCLase6) of polysaccharide lyase family 6 from Microbulbifer sp. ALW1 was cloned and characterized. Optimal temperature and pH for HCLase6 was determined to be 40 ℃ and 5.0, respectively. It displayed good stability at temperature up to 45 ℃ and in the pH range of 4.0-9.0. In addition, HCLase6 demonstrated good tolerance to detergents of Tween 20, Tween 80 and SDS, and was halophilic and halotolerant to Na⁺. Molecular dynamics simulations indicated that the presence of Na⁺ increased the flexibility of the loop region adjacent to the active pocket of HCLase6, altered the surface hydrophobicity and electrostatic potential, and strengthened the motion correlation between amino acid residues. Notably, the enzymatic products of HA oligosaccharides (O-HA) produced by HCLase6 showed significantly enhanced free radical scavenging activities and iron reducing power. They also exhibited the antioxidant activity in human keratinocytes cells after exposure to PM SRM 1648a. This study provides the knowledge of the enzymatic properties of HCLase6 and a reference for its industrial application.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"94"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient stress triggers sugar-mediated carotenoid production in algal-bacterial interactions.","authors":"Abdalah Makaranga, Pannaga Pavan Jutur","doi":"10.1007/s11274-025-04310-0","DOIUrl":"https://doi.org/10.1007/s11274-025-04310-0","url":null,"abstract":"<p><p>This study examined the impact of co-culturing Chlorella saccharophila (UTEX247) with Exiguobacterium sp. strain AMK1 on carotenoid production under nitrate-depleted conditions and 3% CO₂ supplementation. The co-culture significantly enhanced the productivity of lutein (238.31 µg.L⁻¹d⁻¹), zeaxanthin (220.72 µg.L⁻¹d⁻¹), violaxanthin (185.42 µg.L⁻¹d⁻¹), and antheraxanthin (84.07 µg.L⁻¹d⁻¹). Compared to nitrate-repleted mono-cultures, these carotenoids increased by 3.54-fold, 4.81-fold, 12.28-fold, and 9.34-fold, respectively. The violaxanthin cycle, activated by CO₂ supplementation, resulted in higher zeaxanthin production, verified through HPLC analysis. Metabolic profiling highlighted a notable rise in sucrose, an algal-specific metabolite, in the co-culture, reflecting enhanced carbon metabolism and carotenoid synthesis. Conversely, trehalose levels were significantly higher in the bacterial mono-culture (297.77 µg.mL⁻¹) than in the co-culture (88.84 µg.mL⁻¹), showing a 1.68-fold reduction as confirmed by GC-MS/MS. This suggests trehalose as a stress marker, with its reduction indicating mutualistic interactions between algal and bacterial. Overall, the co-culture strategy emerges as a promising approach to activate unexpressed pathways, generate novel metabolites, and enhance yields of valuable carotenoids like lutein and zeaxanthin. This aligns with the principles of a circular bioeconomy, leveraging bacterial biofertilizers, valorizing CO₂, and minimizing chemical dependency, thus offering potential for biorefinery applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"93"},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in Escherichia coli secretion systems for enhanced recombinant protein production.","authors":"Sudarsana Reddy Lokireddy, Sridhar Rao Kunchala, Ramakrishna Vadde","doi":"10.1007/s11274-025-04302-0","DOIUrl":"https://doi.org/10.1007/s11274-025-04302-0","url":null,"abstract":"<p><p>Escherichia coli is inarguably one of the most studied microorganisms across the spectrum of microbiology. It is very widely used in recombinant protein production owing to its rapid growth, ease of genetic manipulation, and relatively high protein yields. Despite all of its advantages, its inability to efficiently secrete proteins naturally remains a drawback leading to protein aggregation as inclusion bodies in the cytoplasm and consequent low overall protein yield. Therefore, many approaches to mitigate this weakness and enhance extracellular secretion to increase protein yield have been devised. This review explores the natural and engineered secretion systems in E. coli, highlighting their potential for enhanced protein secretion for non-glycosylated proteins. Natural one-step (e.g., Type I and III Secretion Systems) and two-step systems (e.g., Sec and Tat pathways) are detailed alongside recent advancements in genetic engineering, mutagenesis, and synthetic biology approaches aimed at improving protein yield, folding, and secretion efficiency. Emerging technologies, such as the ESETEC^® and BacSec^® platforms, promise scalable and cost-effective solutions for higher protein production. Challenges, including limited cellular capabilities and protein aggregation, are addressed through innovative strategies like cell wall modification, co-expression of chaperones, and medium optimization. This review emphasizes E. coli's adaptability to industrial applications, and the promising future of recombinant protein technologies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"90"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the microbial community dynamics and metabolic pathways of primary organic acids in Sichuan Baoning vinegar through metagenomics.","authors":"Aiping Liu, Jie Wu, Jianlong Li, Qin Li, Ning Zhao, Kaidi Hu, Shuliang Liu, Giuseppe Blaiotta, Jiang Zhou","doi":"10.1007/s11274-025-04306-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04306-w","url":null,"abstract":"<p><p>Sichuan Baoning vinegar, a renowned traditional vinegar in China, exhibits a higher lactic acid content compared to acetic acid. The microbiota plays a crucial role in shaping the unique flavor of vinegar, but the species-level succession of key microorganisms and metabolic pathways of major organic acids in this vinegar are still unclear. This study utilized metagenomic sequencing to elucidate microbial succession during fermentation and the functional roles of the microbial community, as well as explore the metabolic network of lactic acid and acetic acid. Our findings revealed that bacteria dominated the fermentation process, with Acetilactobacillus jinshanensis, Lactobacillus amylovorus, and Limosilactobacillus sp. emerging as the top three species. Notably, Acetilactobacillus jinshanensis, Limosilactobacillus sp., Lactobacillus amylovorus, and Limosilactobacillus pontis were key players in lactic acid production, while acetic acid synthesis might be primarily driven by Lactobacillus amylovorus, Limosilactobacillus sp., Lactobacillus acetotolerans, and Acetobacter pasteurianus. This study enhances our understanding of the key microorganisms and organic acids metabolism in vinegar, shedding light on the fermentation mechanism of cereal vinegar.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"91"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of pre-treatments for cost effective production of bioethanol from floral wastes.","authors":"Shalini Rachel, M Shailaja Raj, Maria Shajan","doi":"10.1007/s11274-025-04305-x","DOIUrl":"https://doi.org/10.1007/s11274-025-04305-x","url":null,"abstract":"<p><p>The present study has focused on validating pre-treatment methods for cost effective production of bioethanol from discarded and otherwise waste flowers which are renewable, abundantly available and eco-friendly. Floral waste was collected from various dumpsites and banquet halls and subjected to physical, chemical and biological pre-treatments. Biological pre-treatment by enzymatic hydrolysis using crude cellulase enzyme (5%) yielded 39.4 ± 0.03 g/L of alcohol which is 24.20 and 31.60% more than the alcohol obtained by physical pre-treatment (thermal hydrolysis) and chemical pre-treatment (1% KOH), therefore simultaneous saccharification and fermentation was optimised. A maximum of 396 ± 6.48 g/L bioethanol was obtained after 96 h of fermentation with the isolated yeast, Pichia kudriavzevii CY 902 at pH 5.5 and 37 °C. The minimum ethanol selling price (MESP) of bioethanol produced in our study was enumerated to be 30.43 Rs/L which is 68.31% lesser than the market price of ethanol in India today, making our methodology for production of bioethanol from mixed floral wastes very competitive and cost effective to the existing methodologies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"92"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic review and meta-analysis of the potential effects of endophytic bacteria Klebsiella on plant growth promotion and biocontrol of pathogens.","authors":"Odyone Nascimento da Silva Bandeira, Renato da Silva Bandeira, Cláudia Regina Batista de Souza","doi":"10.1007/s11274-025-04300-2","DOIUrl":"10.1007/s11274-025-04300-2","url":null,"abstract":"<p><p>Klebsiella is a bacterial genus widely recognized in the medical field but with underexplored potential in agriculture. This study employed a systematic review and meta-analysis to investigate scientific articles on plant growth promotion effects associated with endophytic bacteria Klebsiella species. A total of 39 relevant studies, published between 2012 and 2024, were identified based on strict inclusion and exclusion criteria. The analysis revealed that Klebsiella sp., K. pneumoniae, and K. variicola are cosmopolitan species that have functional versatility in phytohormone production, nutrient solubilization, and pathogen control in agricultural systems in both tropical and temperate zones. The data showed a significant correlation between the use of Klebsiella sp. and plant growth, highlighting the positive impact of these species in controlling aggressive pathogens. These findings underscore the potential of Klebsiella as a biotechnological tool for sustainable agricultural practices, enhancing plant growth and reducing the reliance on chemical inputs. The study further emphasizes the need for future research to deepen genomic characterization and expand the agricultural applications of these bacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"89"},"PeriodicalIF":4.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting disease resistance in Solanum melongena L. (eggplant) against Alternaria solani: the synergistic effect of biocontrol Acinetobacter sp. and indole-3-acetic acid (IAA).","authors":"Mohammad Danish, Mohammad Shahid, Zaryab Shafi, Mohammad Tarique Zeyad, Mohammad Abul Farah, Khalid Mashay Al-Anazi, Lukman Ahamad","doi":"10.1007/s11274-025-04282-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04282-1","url":null,"abstract":"<p><p>Alternaria solani causes early blight disease in eggplants, threatening production and leading to significant economic losses. Fungicides are used to control fungal diseases, but their overuse raises resistance concerns. Finding novel, eco-friendly biocontrol agents is therefore a solution for the future. The coordination between antagonistic bacterial agents and plant growth hormones in defense responses against fungal pathogens are crucial. This study assessed biocontrol potential of Acinetobacter sp. SCR-11 (Accession no. OR751536.1) and indole-3-acetic acid (IAA; 100 µM), singly and in combination, against A. solani in eggplants. Strain SCR-11 produced hydrogen cyanide (HCN; 5.7 µg mL⁻¹), siderophore i.e. salicylic acid (14.7 µg mL⁻¹), 2,3-dihydroxybenzoic acid (23.1 µg mL⁻¹) and various extracellular lytic enzymes. Strain SCR-11 exhibited antagonistic activity by strongly inhibiting (82%) A. solani. Acinetobacter sp. inoculation and IAA treatment enhanced growth, biomass, and leaf pigments of A. solani-diseased eggplants, with effectiveness in order: SCR-11 + IAA > SCR-11 > IAA >. The combined treatments (SCR-11 + IAA) most effectively increased total soluble protein (62.5%), carbohydrate (60%), total soluble sugar (81%), and phenol (74%) in A. solani-infected eggplant. Biocontrol agent and IAA application significantly (p ≤ 0.05) reduced proline and malondialdehyde (MDA) levels, alleviating oxidative stress in A. solani-diseased eggplant. The SCR-11 + IAA treatment significantly reduced the percent disease index (71%) and increased protection (69%) in diseased eggplant. The Acinetobacter sp. and IAA coordination enhanced disease resistance in A. solani-infected eggplants by boosting defense enzyme activities (SOD, POD, PAL, and β-1, 3 glucanase), significantly protecting plants from pathogen attack. At harvest, soil populations of A. solani decreased, while SCR-11 populations increased significantly. Acinetobacter sp. and IAA work synergistically through pathogen suppression, plant growth promotion, and induction of plant defense responses. Thus, applying antagonistic PGPR strain with exogenous IAA enhances eggplant resistance to A. solani, providing an environmentally friendly agricultural solution.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"85"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable microbial solutions for managing fungal threats in wheat: progress and future directions.","authors":"Prem Lal Kashyap, Sudheer Kumar, Annie Khanna, Poonam Jasrotia, Gyanendra Singh","doi":"10.1007/s11274-025-04286-x","DOIUrl":"https://doi.org/10.1007/s11274-025-04286-x","url":null,"abstract":"<p><p>Biotrophic and necrotrophic fungi are responsible for causing a range of diseases in wheat, resulting in significant economic losses and a decline in quality. Effective management of these diseases generally involves a combination of resistance breeding, chemical treatments, and cultural practices. However, traditional breeding methods have made limited progress due to the slow pace of genetic improvements, the complexity of the wheat genome, and the quantitative nature of disease resistance traits, along with the constantly evolving virulence of pathogens. This situation has prompted research into more effective and eco-friendly alternatives, such as biological control. Recent studies have concentrated on using antagonistic microbes to decrease the reliance on chemical pesticides while enhancing wheat health and productivity. A comprehensive overview of current knowledge on wheat disease outbreaks is being developed, with a focus on advancements in biological control strategies. The review will first discuss the key fungal pathogens and their associated diseases, followed by a summary of biological control methods, particularly emphasizing potential microbial antagonists. Additionally, it will explore strategies to improve the efficacy of biocontrol agents, which are crucial for a holistic and sustainable approach to wheat disease management. Ultimately, the article will highlight the role of biological control in promoting more sustainable agricultural practices, particularly concerning wheat diseases, in alignment with the UN sustainable development goals.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"79"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}