World journal of microbiology & biotechnology最新文献

{"title":"Adaptive response of the holdase chaperone network of Acidithiobacillus ferrooxidans ATCC 23270 to stresses and energy sources.","authors":"Katherin Izquierdo-Fiallo, Claudia Muñoz-Villagrán, Christian Schimpf, Mick Parra Mardonez, David Rafaja, Michael Schlömann, Mario Tello, Omar Orellana, Gloria Levicán","doi":"10.1007/s11274-025-04325-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04325-7","url":null,"abstract":"<p><p>Acidithiobacillus ferrooxidans is a chemolithoautotrophic acidophilic bacterium belonging to microbial communities involved in sulfide ore bioleaching. This microorganism possesses redundancy of genes encoding ATP-independent chaperone holdases like Hsp20 (hps20.1, hsp20.2, and hsp20.3), Hsp31, Hsp33, RidA (ridA.1 and ridA.2), and Lon (lon.1, lon.2, and lon.3), and single copy genes encoding SlyD and CnoX. We evaluated the response of these holdases to short and long-term stresses induced by changes in temperature (30° to 37 °C), pH (1.6 to 1.2 or 2.0), and oxidative status (1 mM H₂O₂) as well as to different energy sources (iron, sulfur, pyrite, sphalerite or chalcopyrite). Cells adapted under thermal and oxidative stress conditions showed a generalized upregulation of holdase genes, while short-term stress led to more discrete increases in transcript levels, with only hsp20.2 and hsp31 showing higher mRNA levels. hsp31 was also upregulated under acidic stresses, sulfur and sulfides. hsp20 variants showed different mRNA levels under different conditions, and cnoX was induced under oxidative conditions. Cells cultured on chalcopyrite had similar responses to those grown with peroxide. With some exceptions, stresses led to significant increases in intracellular ROS content, and decreases in ATP. These results pave the way to understanding proteostasis systems in extreme acidophilic bacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"121"},"PeriodicalIF":4.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keratinous bioresources: their generation, microbial degradation, and value enhancement for biotechnological applications.","authors":"Vijan Lal Vikash, Numbi Ramudu Kamini, Ganesan Ponesakki, Suresh Kumar Anandasadagopan","doi":"10.1007/s11274-025-04336-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04336-4","url":null,"abstract":"<p><p>Keratin is an important bioresource primarily found in feathers, hair, wool, nails, claws, hooves, horns, and beaks. These crucial protein sources are utilized in many ways for diverse applications. The peptides of keratin develop hierarchical complexity, which leads to the formation of these recalcitrant biomasses. Therefore, microbial breakdown of keratin is a complex process and involves important proteolytic enzymes and inorganic factors. Disulfide bond reduction is the key step in keratin degradation that is mainly facilitated by disulfide-reducing agents or disulfide reductases. Notably, α- and β-keratinous substrates exhibit different structural features; as a result, their disintegration processes make a diversity among keratinous biomass. Various studies have suggested that pretreatment can improve degradation yield following microbial processes. Keratin hydrolysates have been investigated for various uses that contribute to mitigating the environmental impact of these solid wastes. Furthermore, keratin peptides possess bioactive properties, including antioxidant, cytoprotective, and anticancer effects, making them potential candidates for biomedical and nutritional sectors. Microbial keratinases are known for a wide range of substrate specificity that significantly contributes to areas like prion decontamination, carcass processing, antimicrobial functions, and skin exfoliation. This review aims to examine keratin bioresources, their structure, and microbial mechanisms for keratin degradation, along with current insights and future applications of keratin hydrolysates and keratinases.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"118"},"PeriodicalIF":4.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in vanillin synthesis: focusing on microbial synthesis pathways and prospects.","authors":"Yue Zhang, Haoni Luan, Wenhan Qiu, Xue Zhang, Huaishun Wang, Mengyu Liu, Peng Song","doi":"10.1007/s11274-025-04321-x","DOIUrl":"https://doi.org/10.1007/s11274-025-04321-x","url":null,"abstract":"<p><p>Vanillin, often referred to as the 'Queen of Spices', is extensively utilized in the food industry, pharmaceutical manufacturing, cosmetic formulations, agricultural crop protection, and tobacco processing owing to its distinctive flavor properties. In recent years, the biosynthesis of vanillin from suitable substrates through microbial transformation technology has emerged as a prominent research topic, driven by the increasing global demand for \"green and natural\" products and the growing awareness of environmental protection. In this review, we examine the three primary synthesis methods of vanillin: natural extraction, chemical synthesis, and biosynthesis, and elucidate the principles, developmental history, and current technological advancements of each approach. Building on this foundation, the review emphasizes recent advancements in vanillin biosynthesis, analyzes the specific applications, technological innovations, and potential advantages of microbial conversion strategies in vanillin production, and addresses the challenges of low conversion efficiency and weak strain tolerance in microbial synthesis. Furthermore, this review offers insights into the future development trends of vanillin biosynthesis. It proposes research recommendations for optimizing microbial strains, enhancing bioconversion efficiency, and achieving industrial-scale production, aiming to serve as a valuable reference for the continued advancement of vanillin biosynthesis technology.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"111"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the occurrence of Pseudomonas aeruginosa and comprehensive whole genome analysis of the bcsir_p4_s20 strain from municipal wastewater in Chattogram.","authors":"Md Nuruddin Mahmud, Momthahena Momoshed, Md Faisal Ahamed Talukder, Jannatul Ferdous, Farjana Akter Koly, Saiful Islam","doi":"10.1007/s11274-025-04328-4","DOIUrl":"https://doi.org/10.1007/s11274-025-04328-4","url":null,"abstract":"<p><p>Various studies reported the existence of multidrug-resistant (MDR) Pseudomonas aeruginosa in environmental samples, including hospital wastewater, municipal wastewater, and surface water. In this study, we investigated the impact of untreated municipal wastewater transmitting antibiotic-resistant P. aeruginosa strains in wastewater networks of Chattogram City, Bangladesh, through antibiotic susceptibility profiles and whole-genome sequencing (WGS) of the MDR P. aeruginosa bcsir_p4_s20. Forty-two P. aeruginosa isolates were identified from eight locations using polymerase chain reaction (PCR), targeting the oprI and oprL genes, and antibiotic susceptibility was determined against 11 antibiotics by the disc diffusion method. Resistant isolates were identified at all locations, with the highest resistance frequency displayed towards meropenem, cefepime, and colistin. The WGS of bcsir_p4_s20 was performed using the NextSeq 2000 platform. Several bioinformatics tools, like FastQC, Trimmomatic, SPAdes, and Prokka, were used for quality evaluation, low-quality read and adapter filtration, de novo assembly, and functional annotation. Comprehensive Antibiotic Resistance Database (CARD), AMRFinderPlus, and virulence factor database (VFDB) were employed to determine resistance genes and virulence factors. The strain belongs to the O7 serogroup and sequence type ST357. The analysis identified antibiotic resistance genes (blaPDC-11, sul1, and others) that cause resistance through efflux pump and inactivation mechanisms, and virulent genes responsible for adherence (flagella, type IV pili), enzyme (phospholipase C), iron uptake (pyoverdine), secretion system (exoT, exoU), and toxin (toxA) secretion. Therefore, municipal wastewater is a potential reservoir for MDR P. aeruginosa, and establishing wastewater treatment plants (WWTPs) at the primary source points before discharging it to the wastewater network is suggested to mitigate the risk of outbreaks.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"112"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized electroporation for efficient evaluation of genetic elements in Dichomitus squalens.","authors":"Jing Li, Jie Wu, Dongrui You, Yasuo Igarashi, Feng Luo, Peng Chang","doi":"10.1007/s11274-025-04320-y","DOIUrl":"https://doi.org/10.1007/s11274-025-04320-y","url":null,"abstract":"<p><p>Dichomitus squalens, a promising white-rot basidiomycete for industrial enzyme production, necessitates efficient genetic manipulation systems to fully leverage its biotechnological potential. Although established methods such as protoplast-mediated and Agrobacterium tumefaciens-mediated transformations are effective in D. squalens, they are complex and time-consuming. This study introduces the electroporation transformation system for D. squalens, which is simpler and timesaving. By optimizing electroporation parameters, we obtained 77 ± 11 transformants per μg of DNA. Furthermore, we validated the suitability of the Nourseothricin N-acetyl transferase gene as a selectable marker and the NanoLuciferase gene as a bioluminescent reporter in D. squalens using our refined electroporation protocol. This study expands the toolkit for genetic engineering in D. squalens, offering greater flexibility for future molecular investigations. The development of this electroporation system not only enhances the ease of genetic manipulation in D. squalens but also provides a foundation for further exploration of its enzymatic capabilities and potential applications in biotechnology. The streamlined protocol allows for more efficient and rapid genetic engineering, facilitating the study of gene function and the development of improved strains for industrial purposes.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"107"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sludge amended soil induced multidrug and heavy metal resistance in endophytic Exiguobacterium sp. E21L: genomics evidences.","authors":"Mrinmoy Patra, Anand Kumar Pandey, Suresh Kumar Dubey","doi":"10.1007/s11274-025-04323-9","DOIUrl":"https://doi.org/10.1007/s11274-025-04323-9","url":null,"abstract":"<p><p>The emergence of multidrug-resistant bacteria in agro-environments poses serious risks to public health and ecological balance. In this study, Exiguobacterium sp. E21L, an endophytic strain, was isolated from carrot leaves cultivated in soil amended with sewage treatment plant-derived sludge. The strain exhibited resistance to clinically relevant antibiotics, including beta-lactams, fluoroquinolones, aminoglycosides, and macrolides, with a high Multi-Antibiotic Resistance Index of 0.88. Whole-genome sequencing revealed a genome of 3.06 Mb, encoding 3894 protein-coding genes, including antimicrobial resistance genes (ARGs) such as blaNDM, ermF, tetW, and sul1, along with heavy metal resistance genes (HMRGs) like czcD, copB, and nikA. Genomic islands carrying ARGs and stress-related genes suggested potential horizontal gene transfer. The strain demonstrated robust biofilm formation, high cell hydrophobicity (> 80%), and significant auto-aggregation (90% at 48 h), correlating with genes associated with motility, quorum sensing, and stress adaptation. Notably, phenotypic assays confirmed survival under simulated gastrointestinal conditions, emphasizing its resilience in host-associated environments. Comparative genomics positioned Exiguobacterium sp. E21L near Exiguobacterium chiriqhucha RW-2, with a core genome of 2716 conserved genes. Functional annotations revealed genes involved in xenobiotic degradation, multidrug efflux pumps, and ABC-type transporters, indicating versatile resistance mechanisms and metabolic capabilities. The presence of ARGs, HMRGs, and MGEs (mobile genetic elements) highlights the potential role of Exiguobacterium sp. E21L as a reservoir for resistance determinants in agricultural ecosystems. These findings emphasized the need for stringent regulations on sludge-based fertilizers and advanced sludge treatment strategies to mitigate AMR risks in agro-environments.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"114"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the biological production of sugar alcohols from biomass-derived xylose.","authors":"Yue Tang, Xin Ju, Xiaobao Chen, Liangzhi Li","doi":"10.1007/s11274-025-04316-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04316-8","url":null,"abstract":"<p><p>Sugar alcohols are a common class of low-calorie sweeteners. The advancement of technologies utilizing renewable resources has heightened interest in synthesizing sugar alcohols from biomass-derived xylose for cost down of process and sustainability. This review focuses on the potential of biomass-derived xylose and its effective conversion into sugar alcohols, underscoring the significance of this process in sustainable industrial applications. The two main approaches for producing sugar alcohols which include enzyme catalysis and microbial fermentation are thoroughly discussed. The microbial fermentation pathway relies on genetically engineered strains, which are modified to efficiently convert xylose into target sugar alcohols. Enzyme catalysis, on the other hand, directly converts xylose to sugar alcohols through specific reactions. In addition, strategies to improve product selectivity and reduce by-products are discussed in the paper, which are crucial for improving the economic viability and environmental sustainability of sugar alcohol production. Overall, utilizing xylose from biomass to produce sugar alcohols manifests environmental and economic benefits, indicating its substantial potential in the shift towards a low-carbon economy. Future studies may further explore cutting edge technologies to maximize the utilization of biomass-derived xylose and the sustainable production of sugar alcohols.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"110"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of mycobacterial infection by impairing motility and biofilm formation via natural and synthetic molecules.","authors":"Abirami Karthikeyan, Nazia Tabassum, Geum-Jae Jeong, Aqib Javaid, Arun Kumar Mani, Tae-Hee Kim, Young-Mog Kim, Won-Kyo Jung, Fazlurrahman Khan","doi":"10.1007/s11274-025-04322-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04322-w","url":null,"abstract":"<p><p>Mycobacterium species show distinctive characteristics with significant medical implications. Mycobacteria, including Mycobacterium tuberculosis and non-tuberculous mycobacteria, can form biofilms that facilitate their survival in hostile environments and contribute to development of antibiotic resistance and responses by the host immune system. Mycobacterial biofilm development is a complex process involving multiple genetic determinants, notably mmpL genes, which regulate lipid transport and support cell wall integrity, and the groEL gene, which is essential for biofilm maturation. Sliding motility, a passive form of surface movement observed across various mycobacterial species, is closely associated with biofilm formation and colony morphology. The unique sliding motility and biofilm-forming capabilities of Mycobacterium spp. are pivotal for their pathogenicity and persistence in diverse environments. A comprehensive understanding of the regulatory mechanisms governing these processes is crucial for the development of novel therapeutic strategies against mycobacterial infections. This review provides a detailed examination of our current knowledge regarding mycobacterial biofilm formation and motility, with a focus on regulation of these processes, their impact on pathogenicity, and potential avenues for therapeutic intervention. To this end, the potential of natural and synthetic compounds, including nanomaterials, in combating mycobacterial biofilms and inhibiting sliding motility are discussed as well. These compounds offer new avenues for the treatment of drug-resistant mycobacterial infections.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"113"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotransformation of agar extraction waste into cultivation matrix using an adaptively evolved Paenibacillus mucilaginosus strain.","authors":"Hanting Zeng, Jie Wu, Chundong Yu","doi":"10.1007/s11274-025-04332-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04332-8","url":null,"abstract":"<p><p>Industrial agar extraction waste (AEW), which consists of resistant K-rich perlite and agar-dominated seaweed residues, poses environmental challenges. In this study, we isolated K-solubilizing bacteria from an AEW storage yard and identified the adaptively evolved strain, ZK03-Aga1, with efficient K-solubilizing and agar-utilizing properties. Co-fermentation of ZK03-Aga1 with AEW significantly enhanced the production of oligosaccharides, K, and soluble solids. These products, combined with a commercial soilless matrix, form a composite matrix that has been validated for fertility through bok choy planting experiments. The results showed increased bok choy yield, energy, protein, trace element, and chlorophyll content. Bacterial community composition analysis indicated an increase in nitrogen-fixing and organic matter-degrading bacteria. This suggests that AEW nutrients, via ZK03-Aga1 fermentation, directly benefit crops, improving yield, quality, and microbial structure for sustainable fertility. This study presents an efficient method for reusing AEW and mitigating its environmental impacts.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"108"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of three Scenedesmaceae strains: morphology, molecular phylogeny, growth and carotenogenesis.","authors":"Irina N Chubchikova, Natalia V Dantsyuk, Irina V Drobetskaya, Olga N Kuleshova, Elina S Chelebieva","doi":"10.1007/s11274-025-04331-9","DOIUrl":"https://doi.org/10.1007/s11274-025-04331-9","url":null,"abstract":"<p><p>We studied the morphology, molecular phylogeny, growth, and carotenogenesis of three Scenedesmaceae strains (IBSS-12, IBSS-109 and IBSS-112), grown in two-stage batch culture for 18 days. During the \"green\" stage, cells grew in favorable conditions, whereas during the \"red\" stage they were exposed to abiotic stresses. Morphological and molecular analyses showed that strains IBSS-12 and IBSS-109 belong to the genus Coelastrella, and strain IBSS-112 was identified as Desmodesmus. At the \"green\" stage, the maximum cell number was recorded in Desmodesmus (IBSS-112) on day 7, and was twice as high compared to that of Coelastrella strains. All strains showed an almost twofold increase in cell volume and significant dry biomass accumulation (1.5-1.8 g L^- 1). An increase in the carotenoid to chlorophyll ratio (by 1.5-2 times) on day 9, was a signal for switching to the \"red\" stage. Stress conditions caused massive cell death in Desmodesmus IBSS-112, while Coelastrella strains showed a threefold increase in cell number to the end of the experiment. The dry weight increased by 4 and 2.5 times in IBSS-12 and IBSS-109, respectively. The dry biomass productivity for the entire experimental period was 0.32 and 0.25 g L^- 1 day^- 1 in IBSS-12 and IBSS-109, respectively. Pigment analysis revealed typical patterns in green carotenogenic microalgae, with both Coelastrella strains acquiring a bright orange colour and increasing their carotenoid content. IBSS-12 had twice the amount of carotenoids as IBSS-109 did. Carotenoid profiles of Coelastrella strains included valuable ketocarotenoids with a predominance of astaxanthin, canthaxanthin and adonixanthin (19-26, 14-15 and 12-14% in total carotenoids, respectively). The obtained morpho-physiological and biochemical characteristics of the studied strains can serve as additional taxonomic criteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"116"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}