World journal of microbiology & biotechnology最新文献_第2页

Alleviation of mycobacterial infection by impairing motility and biofilm formation via natural and synthetic molecules.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-28 DOI: 10.1007/s11274-025-04322-w

Abirami Karthikeyan, Nazia Tabassum, Geum-Jae Jeong, Aqib Javaid, Arun Kumar Mani, Tae-Hee Kim, Young-Mog Kim, Won-Kyo Jung, Fazlurrahman Khan

{"title":"Alleviation of mycobacterial infection by impairing motility and biofilm formation via natural and synthetic molecules.","authors":"Abirami Karthikeyan, Nazia Tabassum, Geum-Jae Jeong, Aqib Javaid, Arun Kumar Mani, Tae-Hee Kim, Young-Mog Kim, Won-Kyo Jung, Fazlurrahman Khan","doi":"10.1007/s11274-025-04322-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04322-w","url":null,"abstract":"Mycobacterium species show distinctive characteristics with significant medical implications. Mycobacteria, including Mycobacterium tuberculosis and non-tuberculous mycobacteria, can form biofilms that facilitate their survival in hostile environments and contribute to development of antibiotic resistance and responses by the host immune system. Mycobacterial biofilm development is a complex process involving multiple genetic determinants, notably mmpL genes, which regulate lipid transport and support cell wall integrity, and the groEL gene, which is essential for biofilm maturation. Sliding motility, a passive form of surface movement observed across various mycobacterial species, is closely associated with biofilm formation and colony morphology. The unique sliding motility and biofilm-forming capabilities of Mycobacterium spp. are pivotal for their pathogenicity and persistence in diverse environments. A comprehensive understanding of the regulatory mechanisms governing these processes is crucial for the development of novel therapeutic strategies against mycobacterial infections. This review provides a detailed examination of our current knowledge regarding mycobacterial biofilm formation and motility, with a focus on regulation of these processes, their impact on pathogenicity, and potential avenues for therapeutic intervention. To this end, the potential of natural and synthetic compounds, including nanomaterials, in combating mycobacterial biofilms and inhibiting sliding motility are discussed as well. These compounds offer new avenues for the treatment of drug-resistant mycobacterial infections.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"113"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotransformation of agar extraction waste into cultivation matrix using an adaptively evolved Paenibacillus mucilaginosus strain.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-28 DOI: 10.1007/s11274-025-04332-8

Hanting Zeng, Jie Wu, Chundong Yu

{"title":"Biotransformation of agar extraction waste into cultivation matrix using an adaptively evolved Paenibacillus mucilaginosus strain.","authors":"Hanting Zeng, Jie Wu, Chundong Yu","doi":"10.1007/s11274-025-04332-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04332-8","url":null,"abstract":"Industrial agar extraction waste (AEW), which consists of resistant K-rich perlite and agar-dominated seaweed residues, poses environmental challenges. In this study, we isolated K-solubilizing bacteria from an AEW storage yard and identified the adaptively evolved strain, ZK03-Aga1, with efficient K-solubilizing and agar-utilizing properties. Co-fermentation of ZK03-Aga1 with AEW significantly enhanced the production of oligosaccharides, K, and soluble solids. These products, combined with a commercial soilless matrix, form a composite matrix that has been validated for fertility through bok choy planting experiments. The results showed increased bok choy yield, energy, protein, trace element, and chlorophyll content. Bacterial community composition analysis indicated an increase in nitrogen-fixing and organic matter-degrading bacteria. This suggests that AEW nutrients, via ZK03-Aga1 fermentation, directly benefit crops, improving yield, quality, and microbial structure for sustainable fertility. This study presents an efficient method for reusing AEW and mitigating its environmental impacts.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"108"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of three Scenedesmaceae strains: morphology, molecular phylogeny, growth and carotenogenesis.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-28 DOI: 10.1007/s11274-025-04331-9

Irina N Chubchikova, Natalia V Dantsyuk, Irina V Drobetskaya, Olga N Kuleshova, Elina S Chelebieva

{"title":"Study of three Scenedesmaceae strains: morphology, molecular phylogeny, growth and carotenogenesis.","authors":"Irina N Chubchikova, Natalia V Dantsyuk, Irina V Drobetskaya, Olga N Kuleshova, Elina S Chelebieva","doi":"10.1007/s11274-025-04331-9","DOIUrl":"https://doi.org/10.1007/s11274-025-04331-9","url":null,"abstract":"We studied the morphology, molecular phylogeny, growth, and carotenogenesis of three Scenedesmaceae strains (IBSS-12, IBSS-109 and IBSS-112), grown in two-stage batch culture for 18 days. During the \"green\" stage, cells grew in favorable conditions, whereas during the \"red\" stage they were exposed to abiotic stresses. Morphological and molecular analyses showed that strains IBSS-12 and IBSS-109 belong to the genus Coelastrella, and strain IBSS-112 was identified as Desmodesmus. At the \"green\" stage, the maximum cell number was recorded in Desmodesmus (IBSS-112) on day 7, and was twice as high compared to that of Coelastrella strains. All strains showed an almost twofold increase in cell volume and significant dry biomass accumulation (1.5-1.8 g L- 1). An increase in the carotenoid to chlorophyll ratio (by 1.5-2 times) on day 9, was a signal for switching to the \"red\" stage. Stress conditions caused massive cell death in Desmodesmus IBSS-112, while Coelastrella strains showed a threefold increase in cell number to the end of the experiment. The dry weight increased by 4 and 2.5 times in IBSS-12 and IBSS-109, respectively. The dry biomass productivity for the entire experimental period was 0.32 and 0.25 g L- 1 day- 1 in IBSS-12 and IBSS-109, respectively. Pigment analysis revealed typical patterns in green carotenogenic microalgae, with both Coelastrella strains acquiring a bright orange colour and increasing their carotenoid content. IBSS-12 had twice the amount of carotenoids as IBSS-109 did. Carotenoid profiles of Coelastrella strains included valuable ketocarotenoids with a predominance of astaxanthin, canthaxanthin and adonixanthin (19-26, 14-15 and 12-14% in total carotenoids, respectively). The obtained morpho-physiological and biochemical characteristics of the studied strains can serve as additional taxonomic criteria.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"116"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The capacity of the green microalga Chlorella vulgaris in overcoming the detrimental effects of cephalexin contamination.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-28 DOI: 10.1007/s11274-025-04329-3

Veghar Amirian, Morteza Kosari-Nasab, Ali Movafeghi

{"title":"The capacity of the green microalga Chlorella vulgaris in overcoming the detrimental effects of cephalexin contamination.","authors":"Veghar Amirian, Morteza Kosari-Nasab, Ali Movafeghi","doi":"10.1007/s11274-025-04329-3","DOIUrl":"https://doi.org/10.1007/s11274-025-04329-3","url":null,"abstract":"Antibiotics have the potential to affect the health of humans and other living organisms even at slight concentrations. Therefore, there has been a growing global awareness of the environmental impacts associated with antibiotics as emerging pollutants. Cephalexin (CPX) is classified as a first-generation cephalosporin and exhibits a significant efficacy in combating bacterial infections. The current work was conducted to examine the capability of the microalga Chlorella vulgaris to mitigate CPX contamination in aquatic environments. The results indicated that the growth of microalgae diminished in a dose-dependent manner after a 6-day exposure to concentrations of 200-800 mg L- 1 CPX. The analysis conducted through scanning electron microscopy revealed alterations in cell morphology, specifically shrinkage and wrinkling, following the application of CPX. These effects became more significant as the concentration of CPX increased. The results from flow cytometry revealed a notable decrease in cell viability for all concentrations of CPX used, with the highest concentration yielding a viability rate of less than 30%. In addition, CPX caused a decrease in levels of photosynthetic pigments and non-enzymatic antioxidants, including phenols and flavonoids. However, the activity levels of the main antioxidant enzymes considerably increased, achieving their peak at 800 mg L⁻¹. Moreover, the algal cells demonstrated the capability to decrease the concentration of CPX present in the contaminated media, with the most effective reduction observed at 400 mg L- 1. The data obtained confirmed the significant toxicity of CPX on Chlorella vulgaris, while also emphasizing the ability of microalgal cells to withstand antibiotic contamination.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"109"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial synthesis of enantiopure (S)-2-methylbutanoic acid via L-isoleucine catabolism in Bacillus spizizenii.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-28 DOI: 10.1007/s11274-025-04324-8

Jing-Yi Zhao, Fan Gao, Mengru Wu, Yang Li, Yong Chen, Zijun Xiao

{"title":"Microbial synthesis of enantiopure (S)-2-methylbutanoic acid via L-isoleucine catabolism in Bacillus spizizenii.","authors":"Jing-Yi Zhao, Fan Gao, Mengru Wu, Yang Li, Yong Chen, Zijun Xiao","doi":"10.1007/s11274-025-04324-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04324-8","url":null,"abstract":"Enantiopure (S)-2-methylbutanoic acid [(S)-2-MBA] is a high-value chiral compound with applications in fragrances, pharmaceuticals, and agrochemicals. However, conventional chemical synthesis lacks stereoselectivity, while existing biosynthetic methods suffer from low yield and purity. Here, we report a novel microbial process using Bacillus spizizenii ATCC 6633 for efficient (S)-2-MBA production via L-isoleucine catabolism. Through targeted screening of rhizospheric soil isolates and Bacillaceae strains, ATCC 6633 demonstrated superior performance, producing 3.67 g/L (S)-2-MBA with 99.32% enantiomeric excess (ee) under optimized conditions (45 °C, 8% inoculation, 5 g/L glucose, and 8 g/L L-isoleucine). A 58.92% conversion efficiency was achieved, and a simplified purification process recovered 63.90% product with 97.32% purity. Mechanistic studies suggested glucose depletion triggered (S)-2-MBA accumulation, aligning with starvation-induced secondary metabolism. This cost-effective, eco-friendly approach eliminates racemic separation steps and harsh reagents, positioning ATCC 6633 as a promising biocatalyst for sustainable (S)-2-MBA production.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"117"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of a previous infection with Taenia crassiceps cysticerci on the susceptibility to Leishmania (L.) major or L. (V.) braziliensis.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-28 DOI: 10.1007/s11274-025-04327-5

André Murilo de Souza Marques, Santiago Aguiar Espellet Soares, José Rodrigues do Carmo-Neto, Clayson Moura Gomes, Marina Clare Vinaud, Grazzielle Guimarães de Matos, Milton Adriano Pelli de Oliveira

{"title":"Impact of a previous infection with Taenia crassiceps cysticerci on the susceptibility to Leishmania (L.) major or L. (V.) braziliensis.","authors":"André Murilo de Souza Marques, Santiago Aguiar Espellet Soares, José Rodrigues do Carmo-Neto, Clayson Moura Gomes, Marina Clare Vinaud, Grazzielle Guimarães de Matos, Milton Adriano Pelli de Oliveira","doi":"10.1007/s11274-025-04327-5","DOIUrl":"https://doi.org/10.1007/s11274-025-04327-5","url":null,"abstract":"The development of leishmaniasis depends on the ability of Leishmania to invade and survive within macrophages. Macrophages can either promote parasite elimination or support its survival, depending on whether they are classically (M1) or alternatively (M2) activated. Mice chronically infected with Taenia crassiceps cysticerci (TC) develop a predominantly Th2 immune response, which leads to an increased number of M2 macrophages. In this study, we assessed the susceptibility of BALB/c mice previously infected with TC to Leishmania (V.) braziliensis or Leishmania (L.) major. Mice were first inoculated intraperitoneally with TC and after eight weeks infected either L. (V.) braziliensis or L. (L.) major in the paw. We evaluated footpad swelling and parasite load in different organs. We also assessed parasite load in vitro at 3 h, 3, 6, and 9 days; nitric oxide (NO) production, and arginase activity. Macrophages obtained from TC-infected mice (TcMΦ) were more susceptible to L. (V.) braziliensis infection, maintaining a stable parasite load without significant proliferation, while the parasite was killed in thioglycolate-elicited macrophages (TgMΦ). In contrast, L. (L.) major proliferated intensely in TcMΦ, leading to a higher parasite load compared to TgMΦ. In vivo, infection with L. (V.) braziliensis in TC-coinfected mice did not alter the parasite load compared to the group without cysticerci. However, mice infected with L. (L.) major exhibited greater swelling and higher parasite burdens. These findings suggest that infection with TC modulates the immune response of mice but is unable to render resistant mice susceptible to L. (V.) braziliensis.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"115"},"PeriodicalIF":4.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tropical lakes as a novel source of oleaginous yeasts with lipid profiles for biodiesel, oleochemical, and nutraceutical applications.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-13 DOI: 10.1007/s11274-025-04309-7

Mauricio Ramirez-Castrillon, Tatiana Andrea Benavides-León, Lizeth Vanessa Arcos-Velasco, Kriss Dayana Pantoja-Pulido, Lizbeth Lorena Lopez-Parra, Ana Cristina Bolaños-Rojas, Esteban Osorio-Cadavid

{"title":"Tropical lakes as a novel source of oleaginous yeasts with lipid profiles for biodiesel, oleochemical, and nutraceutical applications.","authors":"Mauricio Ramirez-Castrillon, Tatiana Andrea Benavides-León, Lizeth Vanessa Arcos-Velasco, Kriss Dayana Pantoja-Pulido, Lizbeth Lorena Lopez-Parra, Ana Cristina Bolaños-Rojas, Esteban Osorio-Cadavid","doi":"10.1007/s11274-025-04309-7","DOIUrl":"10.1007/s11274-025-04309-7","url":null,"abstract":"Oleaginous yeasts have emerged as promising microbial cell factories for lipid production, offering sustainable alternatives to traditional sources of biodiesel and nutraceuticals. In this study, the lipid accumulation potential of yeast strains isolated from two freshwater aquatic ecosystems in Cali, Colombia, was evaluated to identify novel candidates for biotechnological applications. A total of 56 strains were tested for their oleaginous nature using a gravimetric lipid assay with glucose as a carbon source. Of the assessed strains, 46.15% exceeded 20% lipid yields relative to the dry biomass. Seven strains were selected using glycerol as a carbon source, but only five yeasts were further characterized for their lipid profiles. Molecular identification revealed diverse species, including Aureobasidium sp., Papiliotrema rajashtanensis, Rhodotorula spp., and Clavispora lusitaniae. The selected strains demonstrated unique lipid profiles, with high proportions of monounsaturated and polyunsaturated fatty acids, such as oleic acid (C18:1) and linoleic acid (C18:2). In particular, Aureobasidium sp. accumulated uncommon fatty acids such as petroselinic acid under conditions induced by glycerol. This fatty acid, which has a double bond in position 6,7 and a melting point of 33 °C, highlights its potential as an alternative to margarine production, as well as a precursor to sophorolipids, estolide esters, soaps, and plastics. Rhodotorula sp. exhibited very long-chain fatty acids such as docosadienoic and docosatrienoic acids in its lipid profile. These findings underscore the biotechnological value of yeasts from lentic aquatic systems as sustainable lipid producers, paving the way for innovations in biofuels, nutraceuticals, and oleochemicals.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"105"},"PeriodicalIF":4.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin D3 potentiates antimicrobial and antibiofilm activities of streptomycin and thymoquinone against Pseudomonas aeruginosa.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-13 DOI: 10.1007/s11274-025-04304-y

Priyam Biswas, Soham Bose, Sudipta Chakraborty

{"title":"Vitamin D3 potentiates antimicrobial and antibiofilm activities of streptomycin and thymoquinone against Pseudomonas aeruginosa.","authors":"Priyam Biswas, Soham Bose, Sudipta Chakraborty","doi":"10.1007/s11274-025-04304-y","DOIUrl":"10.1007/s11274-025-04304-y","url":null,"abstract":"Biofilm formed by Pseudomonas aeruginosa is a three dimensional microbial matrix that confers multidrug resistance properties along with the proficiency to evade the host immune system. The present study aims to determine the combinatorial effects of vitamin D3 (cholecalciferol) with two already reported antibiofilm agents: streptomycin and thymoquinone separately against P. aeruginosa biofilms. The minimum inhibitory concentration of streptomycin, thymoquinone and D3 was found to be 20, 10 and 100 μg/mL respectively. The inhibition of biofilm formation and pre-formed biofilm disintegration properties of streptomycin and thymoquinone alone or in combination with D3 at their sub-MIC concentration was determined by crystal violet staining and confocal laser scanning microscopy. A significant inhibition of metabolic activities like oxygen consumption rate and reduction in quorum sensing related cellular activities like swarming motilities, pyocyanin production and extracellular protease secretion by P. aeruginosa were also observed as a result of this combinatorial effect. Both of these combinatorial applications were found to accumulate ROS in bacterial cells, which has been proved to be one of the main causes of their antibiofilm activity. Effect of these two drug combinations on bacterial lettuce leaf infection was also evaluated. Molecular docking analysis indicated that thymoquinone combined D3 can interact more efficiently with the quorum sensing proteins LasI and LasR. The host cell cytotoxicity of these two combinations was found to be negligible on the murine macrophage cell line. These findings suggest that D3 potentiates the antimicrobial and antibiofilm efficacy of both streptomycin and thymoquinone against P. aeruginosa. Although both combinations have shown significant antibiofilm and antimicrobial potential, combinatorial performances of D3 combined thymoquinone were found to be more promising.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"104"},"PeriodicalIF":4.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Causal associations between 26 musculoskeletal disorders and gut microbiota: a Mendelian randomization analysis with Bayesian validation.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-13 DOI: 10.1007/s11274-025-04318-6

Yunhao Wang, Yingze Sun, Hongxing Liao

{"title":"Causal associations between 26 musculoskeletal disorders and gut microbiota: a Mendelian randomization analysis with Bayesian validation.","authors":"Yunhao Wang, Yingze Sun, Hongxing Liao","doi":"10.1007/s11274-025-04318-6","DOIUrl":"10.1007/s11274-025-04318-6","url":null,"abstract":"Emerging evidence suggests that gut microbiota imbalances may influence the onset of musculoskeletal disorders (MSDs), yet conclusive evidence establishing causation remains limited. This study investigates the causal relationship between gut microbiota and a range of MSDs, aiming to identify potential therapeutic targets. Using data on 211 gut microbiome taxa from a genome-wide association study (GWAS) and summary statistics for 26 MSDs from the Finnish Biobank, we employed Mendelian randomization (MR) with inverse-variance weighting (IVW) as the primary analytical approach, complemented by Bayesian model validation to ensure robust results. Our MR analyses revealed significant causal associations between gut microbiota and nine MSDs within four categories, including osteoporosis (IVW-Beta = 0.011, P = 0.025), rheumatoid arthritis (IVW-Beta = - 0.016, P < 0.001), rotator cuff syndrome (IVW-Beta = - 0.007, P = 0.022), and calcific tendonitis of the shoulder (IVW-Beta = - 0.021, P = 0.034). Bayesian validation underscored the plausibility of these relationships, supporting the potential causal role of gut microbiota in the development of these disorders. Our findings present a library of causal associations that underscore the gut microbiome's role in MSD pathogenesis, providing genetic evidence that highlights specific gut microbiota taxa as prospective therapeutic targets. This research offers novel insights into the pathogenic mechanisms underlying MSDs and points toward new directions for future investigation into microbiome-based therapies.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"106"},"PeriodicalIF":4.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic potential and metabolic modulation of Bacillus velezensis VTRNT 01 in response to bacterial elicitors.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-03-12 DOI: 10.1007/s11274-025-04311-z

Thanh-Dung Nguyen, Huu-Nghia Duong, Thi-Pha Nguyen, Phu-Tho Nguyen, Huu-Hiep Nguyen, Thi-Tho Nguyen, Ha-Giang Pham, Dieu-Hien Truong, Huu-Thanh Nguyen

{"title":"Antibiotic potential and metabolic modulation of Bacillus velezensis VTRNT 01 in response to bacterial elicitors.","authors":"Thanh-Dung Nguyen, Huu-Nghia Duong, Thi-Pha Nguyen, Phu-Tho Nguyen, Huu-Hiep Nguyen, Thi-Tho Nguyen, Ha-Giang Pham, Dieu-Hien Truong, Huu-Thanh Nguyen","doi":"10.1007/s11274-025-04311-z","DOIUrl":"10.1007/s11274-025-04311-z","url":null,"abstract":"Bacterial elicitors are recognized for their ecological role in stimulating plant defenses and enhancing the production of beneficial metabolites. This study explores the antibiotic potential of endophytic Bacillus velezensis VTRNT 01, isolated from Adenosma bracteosum Bonati, under co-cultivation with bacterial elicitors (Staphylococcus aureus, Escherichia coli, and Aeromonas hydrophila). By leveraging these interactions, we aim to unlock the full potential of endophytic bacteria for sustainable applications in agriculture and pharmaceuticals. Using gas chromatography-mass spectrometry (GC-MS) analysis, we identified a total of 42 distinct chemical compounds produced under these conditions. Notably, 15 of these compounds were exclusively induced by the elicitor treatment, suggesting a strong interactive effect between Bacillus velezensis and the elicitors. Among the identified compounds, several have well-documented antimicrobial properties, including benzaldehyde, benzeneacetic acid, and tetradecanoic acid, which were shown to exhibit significant antibacterial activity against common pathogens. These findings demonstrate the potential of bio-elicitor strategies to enhance the biosynthesis of antimicrobial compounds, paving the way for innovative solutions in crop protection and the development of new therapeutic agents.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"102"},"PeriodicalIF":4.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0