World journal of microbiology & biotechnology最新文献

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The potential of Paraburkholderia species to enhance crop growth.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-02-05 DOI: 10.1007/s11274-025-04256-3
Fernando Uriel Rojas-Rojas, Ingrid Melissa Gómez-Vázquez, Paulina Estrada-de Los Santos, Harumi Shimada-Beltrán, Julio C Vega-Arreguín
{"title":"The potential of Paraburkholderia species to enhance crop growth.","authors":"Fernando Uriel Rojas-Rojas, Ingrid Melissa Gómez-Vázquez, Paulina Estrada-de Los Santos, Harumi Shimada-Beltrán, Julio C Vega-Arreguín","doi":"10.1007/s11274-025-04256-3","DOIUrl":"10.1007/s11274-025-04256-3","url":null,"abstract":"<p><p>Agrochemicals are the primary alternative for maintaining the high yields necessary to produce sufficient plant-based foods to supply the world population. In recent decades, one of the most extensively explored alternatives to replace agrochemicals and reduce their environmental impact has been the use of microorganism-based products to boost crop yields with less environmental impact. This review focuses on the results of studies that have demonstrated the potential of the genus Paraburkholderia to increase crop yields and be utilized in biofertilizers and biocontrol products. A literature search was performed electronically considering articles and books published until August 19, 2024. We identified 24 species of Paraburkholderia with the ability to improve crop yields after their inoculation by different methods on seeds, seedlings, plantlets, adult crops, or fruits. The effects of these bacteria have been tested under laboratory, greenhouse, or field conditions. These Paraburkholderia species mediate their positive impact on crop growth by direct and indirect plant growth-promoting mechanisms, which include improving nutrient uptake, stimulating growth by phytohormone production, regulation and stimulation of metabolic pathways, induction of abiotic stress tolerance, and disease control by direct pathogen inhibition or induction of systemic resistance in plants. The literature reviewed here supports the use of Paraburkholderia in bio-inputs under the actual panorama of climate change and the necessity to increase sustainable agriculture worldwide.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"62"},"PeriodicalIF":4.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic insights into habitat adaptation of Lactobacillus species.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-02-04 DOI: 10.1007/s11274-025-04275-0
Alejandra Mejía-Caballero, Rafael López-Sánchez, Blanca Ramos-Cerrillo, Alejandro Garciarrubio, Lorenzo Segovia
{"title":"Genomic insights into habitat adaptation of Lactobacillus species.","authors":"Alejandra Mejía-Caballero, Rafael López-Sánchez, Blanca Ramos-Cerrillo, Alejandro Garciarrubio, Lorenzo Segovia","doi":"10.1007/s11274-025-04275-0","DOIUrl":"10.1007/s11274-025-04275-0","url":null,"abstract":"<p><p>Lactobacillus is one of the most important genera within the lactic acid bacteria group, due to its importance in the food industry and the health field. This diversity can be explained either by their radiation in different environments or by the domestication process in artificial habitats, such as fermented foods. In this study, we performed a comparative genomic analysis of 1020 Lactobacillus genomes, categorizing them into five broad habitats: insects, vertebrates (including humans and animals), vegetables, free-living environments, and dairy products. Utilizing phylogenetic relationships, genomic distances, and gene presence/absence profiles, we identified distinct clustering patterns associated with specific environmental adaptations. Notably, species within the Lactobacillus delbrueckii clade exhibited GC content variations fivefold greater than those observed in other bacterial genera, indicating significant genomic divergence. Insect-associated species showed a strong correlation between genes for carbohydrate utilization and those for amino acid biosynthesis across all habitats. However, individual gene analyses revealed no consistent correlation between habitat adaptation and phylogenetic proximity, suggesting that Lactobacillus employs strain-specific adaptive mechanisms rather than universal genetic markers. Notably, around 50% of the genes associated with specific habitats are hypothetical. Our findings highlight the genomic complexity of Lactobacillus, driven by diverse adaptive strategies, and underscore the need for more comprehensive sampling to fully elucidate the evolutionary dynamics within this important genus.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"61"},"PeriodicalIF":4.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11790720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biotechnological uses of purified and characterized alkaline cellulase from extremophilic Bacillus pumilus VLC7 from Lake Van.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-02-04 DOI: 10.1007/s11274-025-04271-4
Aysun Ayse Yilmaz, Sumeyra Gurkok
{"title":"Biotechnological uses of purified and characterized alkaline cellulase from extremophilic Bacillus pumilus VLC7 from Lake Van.","authors":"Aysun Ayse Yilmaz, Sumeyra Gurkok","doi":"10.1007/s11274-025-04271-4","DOIUrl":"10.1007/s11274-025-04271-4","url":null,"abstract":"<p><p>The present study focuses on the isolation and characterization of a bacterium from Lake Van adapted to an alkaline pH environment with significant cellulase production potential. The identified isolate, Bacillus pumilus VLC7 (GenBank Acc No: OR415888.1), exhibited the highest cellulase activity among other alkaliphilic isolates. The purification employing protein precipitation by ammonium sulfate, ultrafiltration, and ion exchange chromatography resulted in 20-fold purification with a specific activity of 16 U/mg protein. Cellulase had a molecular weight of 76 kDa, 3.13 mM K<sub>m</sub> and 0.160 U/mg V<sub>max</sub> values. The enzyme displayed maximum activity at pH 9.0 and 40 °C and retained at least 80% of its activity at temperatures of 25-60 °C for 90 min and pH 4-12 for one hour. Stability tests also revealed the enzyme's resilience to various reagents, metal ions, organic solvents, and detergents. Furthermore, the biotechnological applications of cellulase were explored, demonstrating its effectiveness in fabric biopolishing (removing pilling), as well as in the removal of fabric dyes. The research findings underscore the potential of B. pumilus VLC7 as a valuable source for eco-friendly industrial biotechnology applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"60"},"PeriodicalIF":4.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11790762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advancing wound healing: controlled release of tannic acid via epitope imprinted antimicrobial spongy cover material.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-02-04 DOI: 10.1007/s11274-025-04266-1
Büşra Tuna, Pırıl Arısoy, Hatice İmge Oktay Başeğmez, Gözde Baydemir Peşint
{"title":"Advancing wound healing: controlled release of tannic acid via epitope imprinted antimicrobial spongy cover material.","authors":"Büşra Tuna, Pırıl Arısoy, Hatice İmge Oktay Başeğmez, Gözde Baydemir Peşint","doi":"10.1007/s11274-025-04266-1","DOIUrl":"10.1007/s11274-025-04266-1","url":null,"abstract":"<p><p>The increasing resistance of microorganisms to conventional antibiotics calls for alternative antimicrobial strategies. This study introduces a novel approach to acute wound healing by incorporating epitope-imprinted spongy cover materials with antimicrobial properties, using Tannic acid (TA) as the active agent within biocompatible cryogels imprinted with gallic acid. The spongy materials were synthesized and characterized through Fourier Transform Infrared Spectroscopy (FTIR), swelling tests, and Scanning Electron Microscopy (SEM) to assess their structural and physicochemical properties. The antimicrobial efficacy of the cryogels, loaded with 1.5, 3, 5 mg/mL of TA concentrations, was tested against Staphylococcus aureus and Escherichia coli, common pathogens in wound infections. The highest inhibition zone was determined to be 15 mm for S. aureus and 12 mm for E. coli. Maximum TA adsorption was 210.27 mg/g for eMIP and 24.74 mg/g for NIP. Cumulative release studies revealed the highest release rate occurred within the first 2 h. TA release kinetics indicated a non-Fickian diffusion mechanism. Additionally, the biocompatibility and potential cytotoxicity of the spongy materials, including TA-loaded variants, were assessed using the MTT assay on cultured cells. The results confirmed that the spongy materials are non-toxic and do not inhibit cell proliferation, supporting their suitability for acute wound healing. This study demonstrates that TA-loaded epitope-imprinted Poly(2-hydroxyethyl methacrylate) (pHEMA)-based spongy materials possess antimicrobial properties, making them potential candidates for wound and burn dressing applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"59"},"PeriodicalIF":4.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11790693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficient secretory expression, purification, and characterization of lipase in Pseudomonas aeruginosa M18, with multifunctional applications in diagnostics.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-01-31 DOI: 10.1007/s11274-025-04279-w
Yunbo Liu, Pan Zhu, Lingyuan Kong, Jing Wang, Chengdong Ji, Yuanli Li, Lichun Dong, Weijing Yi
{"title":"Efficient secretory expression, purification, and characterization of lipase in Pseudomonas aeruginosa M18, with multifunctional applications in diagnostics.","authors":"Yunbo Liu, Pan Zhu, Lingyuan Kong, Jing Wang, Chengdong Ji, Yuanli Li, Lichun Dong, Weijing Yi","doi":"10.1007/s11274-025-04279-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04279-w","url":null,"abstract":"<p><p>Lipase (EC 3.1.1.3) is a crucial hydrolase with broad industrial and clinical applications. In this study, the lipA and lipH genes from Pseudomonas aeruginosa were cloned into the pBBR1MCS-2 vector and expressed under the regulation of the highly efficient BSFP_0720 promoter from Burkholderia stabilis FERMP-21014. This system allowed for efficient secretory expression in Pseudomonas aeruginosa without requiring an inducer. The recombinant lipase exhibited both lipase and cholesteryl esterase activities, making it suitable for triglyceride and cholesterol assay kits. Additionally, gene editing was used to knock out the endogenous cholesterol oxidase gene in Pseudomonas aeruginosa, eliminating cross-interference in different assay kits. High-density fermentation using glucose as the carbon source resulted in lipase activity reaching 68 kU/L and cholesteryl esterase activity reaching 214 kU/L after 30 h of fermentation, representing a 356-fold increase compared to natural production. By combining ammonium sulfate precipitation, hydrophobic interaction chromatography, and anion exchange chromatography, a purity of 94.32% was achieved (as determined by CE-SDS). Accelerated stability tests showed that the lyophilized lipase retained over 96% residual activity after storage at 37 °C for 21 days and at 45 °C for 7 days, suggesting its suitability for long-term storage. The enzymatic properties of the lipase demonstrated resistance to common chemicals, high activity in buffers with pH values between 7 and 9, and short-term tolerance to high temperatures (60 °C). These characteristics make the lipase highly adaptable for use in complex clinical samples and various industrial applications. The successful high-efficiency expression and multifunctional utility of this lipase highlight its significant commercial potential in diagnostics and other fields.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"57"},"PeriodicalIF":4.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Statistical optimisation and analysis of biomass and exopolysaccharide production by Lacticaseibacillus rhamnosus LRH30.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-01-31 DOI: 10.1007/s11274-025-04273-2
Helena Mylise Copeland, Susan Maye, George MacLeod, Dermot Brabazon, Christine Loscher, Brian Freeland
{"title":"Statistical optimisation and analysis of biomass and exopolysaccharide production by Lacticaseibacillus rhamnosus LRH30.","authors":"Helena Mylise Copeland, Susan Maye, George MacLeod, Dermot Brabazon, Christine Loscher, Brian Freeland","doi":"10.1007/s11274-025-04273-2","DOIUrl":"10.1007/s11274-025-04273-2","url":null,"abstract":"<p><p>Exopolysaccharides (EPS) produced by lactic acid bacteria with immunomodulatory potential are promising natural food additives. This study employs small-scale, 250 mL bioreactors combined with a central composite design to optimise two important bioprocess parameters, namely temperature and airflow, to achieve high yields of biomass and EPS from Lacticaseibacillus rhamnosus LRH30 (L. rhamnosus LRH30). A quadratic model was determined to be the best fit for the production of both products. The optimum critical process parameters for maximised biomass were identified to be 37.01 °C with an airflow of 0.12 vvm, while optimum criteria was 20.1 °C with an airflow of 0.18 vvm for maximum EPS production. Under these optimized conditions, small-scale batch experiments yielded a biomass concentration of 10.1 g/L and an EPS yield of 520.2 mg/L. In comparison, scale-up experiments in 2L reactors resulted in a biomass concentration of 8.54 g/L (a reduction of 18%) and an EPS yield of 654.6 mg/L (an increase of 26%). The produced EPS was purified and characterised through Fourier transform infrared spectroscopy and showed characteristic peaks associated with polysaccharides. The immunomodulatory potential of the L. rhamnosus LRH30 cells and EPS was evaluated through cytokine and chemokine secretion in a J774A.1 murine macrophage, resulting in a predominantly anti-inflammatory effect of L. rhamnosus LRH30 and EPS.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"58"},"PeriodicalIF":4.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of glycosylation in bacterial resistance to carbapenems.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-01-30 DOI: 10.1007/s11274-025-04272-3
Jing Dang, Xinlu Yu, Mengyuan Zhang, Liuyi Dang, Zheng Li, Jian Shu
{"title":"Role of glycosylation in bacterial resistance to carbapenems.","authors":"Jing Dang, Xinlu Yu, Mengyuan Zhang, Liuyi Dang, Zheng Li, Jian Shu","doi":"10.1007/s11274-025-04272-3","DOIUrl":"https://doi.org/10.1007/s11274-025-04272-3","url":null,"abstract":"<p><p>Carbapenems are a class of β-lactam antibacterial drugs with a broad antibacterial spectrum and strong activity, commonly used to treat serious bacterial infections. However, improper or excessive use of carbapenems can lead to increased bacterial resistance, which is a significant concern as they are often used as last resort for treating multidrug-resistant (MDR) gram-negative bacteria. Confronted with this challenge, it is crucial to comprehensively understand the mechanism of carbapenem resistance to develop effective therapeutic strategies and innovative drugs. In recent years, emerging research on the glycosylation of bacterial proteins has highlighted the crucial role of glycans in various bacterial processes, including carbapenem resistance. Given the limited understanding of bacterial glycosylation, its role in in carbapenem resistance may be more pivotal than currently acknowledged. In this review, we summarize the direct and multifunctional role of glycosylation in bacterial resistance as well as the classical and recently reported mechanisms of bacterial carbapenem resistance, focusing on illuminating the potential role of glycosylation in carbapenem resistance. We also discuss the potential of leveraging this knowledge to develop more effective strategies for combating clinically resistant bacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"55"},"PeriodicalIF":4.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning and functional characterization of sesquiterpene synthase genes from Inonotus obliquus using a Saccharomyces cerevisiae expression system.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-01-30 DOI: 10.1007/s11274-025-04274-1
Qiao Liu, Junzhi Peng, Ziling Tao, Jiluan Zhang, Weifan Wu, Zhiwu Tan, Tao Zhou, Xiaoying Cao, Jihong Jiang
{"title":"Cloning and functional characterization of sesquiterpene synthase genes from Inonotus obliquus using a Saccharomyces cerevisiae expression system.","authors":"Qiao Liu, Junzhi Peng, Ziling Tao, Jiluan Zhang, Weifan Wu, Zhiwu Tan, Tao Zhou, Xiaoying Cao, Jihong Jiang","doi":"10.1007/s11274-025-04274-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04274-1","url":null,"abstract":"<p><p>Inonotus obliquus (Chaga mushroom) is a large medicinal and edible fungus that contains a wealth of bioactive terpenoids. However, the detection of certain low-abundance sesquiterpenoids remains a challenge due to limitations in extraction and analytical techniques. Furthermore, the synthase genes responsible for the biosynthesis of the identified terpenoids have not yet been clearly elucidated. To address this, our study combined transcriptome mining with yeast heterologous expression to investigate the synthase genes involved in sesquiterpenoid production in I. obliquus. We successfully identified eight sesquiterpene synthase genes and one farnesyltransferase. Among these, only cis-β-farnesene, synthesized by IoTPS2, had been previously detected before in the sclerotium of I. obliquus, while the other nine sesquiterpenoids-including neoisolongifolene-8-ol, β-longipinene, vetiselinenol, isolongifolene, 7,8-dehydro-8a-hydroxy-, 4a,8b,10b,11a-tetramethylbicyclo[6.3.0]undec-1-en-5-one, 6,11-oxido-acor-4-ene, β-maaliene, neointermedeol, and longifolenaldehyde-were discovered for the first time. This research provides a critical scientific foundation for expanding the known repertoire of sesquiterpenoids and their corresponding synthase genes in I. obliquus.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"56"},"PeriodicalIF":4.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Current insights into molecular mechanisms of environmental stress tolerance in Cyanobacteria.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-01-29 DOI: 10.1007/s11274-025-04260-7
Preeti Rai, Ruchi Pathania, Namrata Bhagat, Riya Bongirwar, Pratyoosh Shukla, Shireesh Srivastava
{"title":"Current insights into molecular mechanisms of environmental stress tolerance in Cyanobacteria.","authors":"Preeti Rai, Ruchi Pathania, Namrata Bhagat, Riya Bongirwar, Pratyoosh Shukla, Shireesh Srivastava","doi":"10.1007/s11274-025-04260-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04260-7","url":null,"abstract":"<p><p>The photoautotrophic nature of cyanobacteria, coupled with their fast growth and relative ease of genetic manipulation, makes these microorganisms very promising factories for the sustainable production of bio-products from atmospheric carbon dioxide. However, both in nature and in cultivation, cyanobacteria go through different abiotic stresses such as high light (HL) stress, heavy metal stress, nutrient limitation, heat stress, salt stress, oxidative stress, and alcohol stress. In recent years, significant improvement has been made in identifying the stress-responsive genes and the linked pathways in cyanobacteria and developing genome editing tools for their manipulation. Metabolic pathways play an important role in stress tolerance; their modification is also a very promising approach to adapting to stress conditions. Several synthetic as well as systems biology approaches have been developed to identify and manipulate genes regulating cellular responses under different stresses. In this review, we summarize the impact of different stresses on metabolic processes, the small RNAs, genes and heat shock proteins (HSPs) involved, changes in the metabolome and their adaptive mechanisms. The developing knowledge of the adaptive behaviour of cyanobacteria may also be utilised to develop better stress-responsive strains for various applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"53"},"PeriodicalIF":4.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and structure determination of a new antibacterial lanthipeptide derived from the marine derived bacterium Lysinibacillus sp.CTST325.
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-01-29 DOI: 10.1007/s11274-024-04212-7
Chanaphat Thetsana, Ryota Moriuchi, Shinya Kodani
{"title":"Isolation and structure determination of a new antibacterial lanthipeptide derived from the marine derived bacterium Lysinibacillus sp.CTST325.","authors":"Chanaphat Thetsana, Ryota Moriuchi, Shinya Kodani","doi":"10.1007/s11274-024-04212-7","DOIUrl":"https://doi.org/10.1007/s11274-024-04212-7","url":null,"abstract":"<p><p>Marine resources are attractive for screening new useful bacteria. From a marine sediment sample, we performed isolation and screening of bacterial strains in search of new bioactive compounds. HPLC and ESI-MS analysis indicated that the new bacterium, Lysinibacillus sp. CTST325 (NBRC 116944), produced a new peptidic compound, lysinibacin. Genome sequence analysis of Lysinibacillus sp. CTST325 indicated the presence of several biosynthetic gene clusters for secondary metabolites, including lanthipeptides. The structure determination of lysinibacin was performed using CID-MS and NMR spectral data. As a result, lysinibacin was identified as a new class III lanthipeptide, containing N-dimethylated Tyr at the N-terminus and the unusual amino acid labionin at the C-terminus. The biosynthetic gene cluster of lysinibacin was identified from the genome data of the strain CTST325, based on the structure of lysinibacin. Lysinibacin showed antibacterial activity against Gram-positive bacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"54"},"PeriodicalIF":4.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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