World journal of microbiology & biotechnology最新文献

{"title":"Functional and genomic characterization of halotolerant Bacillus paramycoides MSR1 for MICCP-based durability enhancement of cementitious materials under saline conditions.","authors":"Shivangi Gautam, Bhavdeep Sharma, M Sudhakara Reddy","doi":"10.1007/s11274-026-05321-1","DOIUrl":"10.1007/s11274-026-05321-1","url":null,"abstract":"<p><p>Microbially induced calcium carbonate precipitation (MICCP) is a sustainable strategy for improving the mechanical strength and durability of cementitious materials through biologically mediated mineral precipitation. The performance of most ureolytic bacteria declines under saline conditions, limiting their use in marine environments. This study evaluates the genomic and functional adaptation of the halotolerant ureolytic bacterium Bacillus paramycoides MSR1, isolated from Sambhar Lake, Rajasthan, India, for MICCP-based applications in saline construction environments. B. paramycoides retained high urease (590 U/mL) and carbonic anhydrase (90 U/mL) activities and precipitated 180 mg/100 mL of calcium carbonate after 7 days at 3.5% NaCl concentration, equivalent to seawater salinity, while continuing to demonstrate effective biomineralization up to 5% NaCl concentration. Therefore, 3.5% NaCl was selected for mortar casting and curing to simulate marine conditions. Incorporation of B. paramycoides in mortar specimens cast and cured under 3.5% saline conditions (BS) increased 28-day compressive strength by 15.28% and reduced sorptivity from 0.0043 to 0.0007 cm s^- 1/2 compared to control mortar (W). FE-SEM/EDX analysis confirmed CaCO₃ deposition in mortar matrix of BS specimens. Whole-genome sequencing produced an assembly of 5,453,313 bp with N₅₀ of 129,914 bp, comprising 5,832 coding sequences. Genome annotation identified urease structural (ureA, ureB, ureC) and accessory (ureD, ureE, ureF, ureG) genes along with carbonic anhydrase genes and a broad range of salt adaptation genes involved in compatible solute biosynthesis and transport, ion homeostasis, mechanosensitive channels and stress response regulation, providing molecular insight into its biomineralization potential. The physiological, material and genomic analyses demonstrate that B. paramycoides can sustain biomineralization under saline stress, highlighting its potential for developing durable and self-healing cementitious materials for coastal and marine infrastructure.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface sampling and transport for an effective microbial forensics: recovery of bacterial nucleic acid and protein from adhesive tape.","authors":"Snehasri Motamarry, Pooja Yadav, Ravi Bhushan Kumar, Veer Vikram Prakash, Syed Imteyaz Alam","doi":"10.1007/s11274-026-05306-0","DOIUrl":"10.1007/s11274-026-05306-0","url":null,"abstract":"<p><p>Microbial forensic analysis for deliberate, accidental and natural release of pathogens entail serious ramifications on human health. Surface sampling and transport for downstream verification of biological agents is posed with challenges including presence of background microbial consortium, delay in transport from remote location, and vulnerability of biomolecules to compromised cold chain. Glass surface pre-exposed to field conditions was spiked with S. typhi and sampled with swab. Storage of swab in VTM, PBS, alkaline lysis buffer, and under desiccated condition at RT and 10 °C for a period of 3 days indicated significantly higher DNA recovery for swabs stored under desiccated conditions. Recovery of bacterial protein from three transport media indicated pronounced stability at both temperatures. Tape-lift was envisaged as the method of surface sampling for providing stability to analytes during transport. Post-spiking (S. typhi) incubation of adhesive tapes for 3 and 7 days at RT indicated DNA recovery on both the time points and extracted DNA was amenable to PCR amplification of target specific gene. Methodology was also optimized for recovery of bacterial protein from adhesive tapes using S. typhi and B. anthracis Sterne spores. Protein stability was observed for both the agents after 3 and 7 days of storage at RT. The proteins extracted from the spiked adhesive tapes could be successfully identified using high resolution mass spectrometry (HRMS) with significant proteome coverage (84% on 3 d and 60% on 7 d). Application of adhesive tapes for sampling smooth surfaces for extraction of bacterial nucleic acid and protein suggest stability of analytes even without cold chain.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of antibiotic resistance and virulence profiles of exoU and exoS Pseudomonas aeruginosa isolates from urinary tract infections and microbial keratitis in Bangladesh and India.","authors":"Tanzina Akter, Shiful Islam, Abrar Maswood Haider, Rifaquat Ahmed, Kaniz Fatema, Md Mozammel Hoque, Fiona Stapleton, Mark Willcox","doi":"10.1007/s11274-026-05320-2","DOIUrl":"10.1007/s11274-026-05320-2","url":null,"abstract":"<p><p>Pseudomonas aeruginosa is an important opportunistic pathogen in urinary tract infections (UTIs) and microbial keratitis (MK), but differences in resistance and virulence between exoU and exoS P. aeruginosa isolates across regions and infection sites remain poorly understood. This study compared antibiotic resistance and virulence profiles of exoU and exoS P. aeruginosa isolates from Bangladeshi and Indian UTIs, and from Indian UTIs and MK collections. A total of 23 Bangladeshi UTI isolates were analyzed by broth microdilution for susceptibility to ciprofloxacin, levofloxacin, gentamicin, and tobramycin, and by whole-genome sequencing (WGS) to identify resistance-associated mutations, acquired resistance genes, and virulence factors. These WGS data were compared with 15 publicly available Indian UTI genomes, while the Indian UTI genomes were compared with 19 publicly available Indian MK genomes. In the Bangladeshi UTI cohort, exoU isolates showed greater resistance to fluoroquinolones and aminoglycosides than exoS isolates. Comparative analysis of Bangladeshi versus Indian UTI isolates and Indian UTI versus MK isolates showed that exoU isolates carried a burden of resistance mutations, including enrichment of GyrA Thr83Ile, ParE Asp533Glu, MexR Val126Glu, MexB Ser1041Glu, and MexC Ala262Glu. Aminoglycoside-associated substitutions FusA2 Gln161Leu and ParR Leu153Arg and Ser170Asn were more common in exoU UTI isolates. Acquired resistance genes showed aac(6')-II and aac(3)-Id were linked to Bangladeshi UTI, rmtF associated with Indian UTI, and crpP confined to MK exoU isolates. Virulence profiling showed exoU enrichment in pldA, pvdY, and csrA, whereas exoS was associated with pilC, pilV, pilW, pilY2, and pilA. Collectively, these results indicate that exoU and exoS isolates differ in both their resistance and virulence profiles, while also showing evidence of geographic and infection-site-specific adaptation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocess development for enhanced bioethanol production from Saccharum spontaneum biomass using synergistic ionic liquid-metal salt pretreatment and enzymatic catalysis.","authors":"Surbhi Vaid, Surbhi Sharma, Bisma Habib, Arpana Thakur, Muskaan Chib, Priya Singh, Nisha Kapoor, Ritu Mahajan, Sumit G Gandhi, Bijender Kumar Bajaj","doi":"10.1007/s11274-026-05303-3","DOIUrl":"10.1007/s11274-026-05303-3","url":null,"abstract":"<p><p>Lignocellulosic biomass (LB) is a renewable and abundant feedstock with great potential for biofuel production. The current study is the first demonstration of a synergistic pretreatment strategy that integrates ionic liquid (IL) 1-ethyl-3-methylimidazolium bromide ([Emim][Br]) and metal salt FeSO₄ for next generation bioconversion of kans grass biomass (KGB; Saccharum spontaneum) into bioethanol through a one-pot consolidated bioprocess (OPCB). An indigenously developed IL-stable and efficient cellulase-xylanase enzyme preparation from Bacillus subtilis G₂ was employed for saccharification of the pretreated biomass. Combined [Emim][Br] + FeSO₄ pretreatment coupled with enzymatic hydrolysis yielded a significantly higher sugar yield (113.71 mg/g KGB) than the individual pretreatments with FeSO₄ (48.40 mg/g KGB) or [Emim][Br] (50.74 mg/g KGB), thus, demonstrating a clear synergistic enhancement in biomass deconstruction and saccharification efficiency. Optimization of pretreatment and saccharification parameters enhanced the reducing sugar yield considerably (2.42-fold) i.e. 274.77 mg vs. 113.74 mg/g biomass in control. Subsequent fermentation of KGB sugars generated 0.09 g/g biomass of ethanol, with a fermentation efficiency of 66.75%, and a bioconversion efficiency of 32.54%. Detailed characterization of the pretreated biomass using techniques/methods such as, XRD (X-ray Diffraction), FT-IR (Fourier Transform Infrared Spectroscopy), NMR (Nuclear Magnetic Resonance Spectroscopy), SEM (Scanning Electron Microscopy), BET (Brunauer-Emmett-Teller), water retention capacity, and cellulase adsorption isotherm reveals the effectiveness of the combined pretreatment strategy. The current findings highlight the significance of [Emim][Br] and FeSO₄ as an effective and synergistic pretreatment system for improving the bioconversion of KGB into bioethanol.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plastic-algae interactions in freshwater and marine systems: mechanisms, evidence and prospects for bioremediation.","authors":"Sagaya John Paul Joseph, Sathish Kumar Boopathi, Nagaraj Subramani","doi":"10.1007/s11274-026-05322-0","DOIUrl":"10.1007/s11274-026-05322-0","url":null,"abstract":"<p><p>Plastic pollution is among this century's defining environmental problems, and aquatic systems carry a disproportionate share. As polymers fragment into microplastics and nanoplastics they disperse through rivers, lakes and oceans, harming organisms and entering food webs. Algae have attracted growing attention as a biological complement to physical and chemical clean-up. This review synthesises peer-reviewed studies published between 2015 and 2026 on interactions between plastics and freshwater and marine algae. We examine eukaryotic microalgae from freshwater (Chlorella, Scenedesmus, Coccomyxa, Uronema) and marine systems (Navicula, Phaeodactylum, Skeletonema, Dunaliella); cyanobacteria (Phormidium, Oscillatoria, Anabaena, Nostoc, Arthrospira), treated separately as prokaryotes; and the macroalga Sargassum. Reported outcomes range widely from a few percent of low-density polyethylene surface-altered by cyanobacteria, to roughly a quarter of mixed-polymer film attributed to Coccomyxa, and up to ~ 27% of high-density polyethylene by a freshwater epiplastic consortium, along with many studies that document surface colonisation, hetero-aggregation and physiological stress rather than complete breakdown. We explain the mechanisms involved: extracellular enzymes proposed to cleave polymer chains, the extracellular polymeric substances that anchor cells to plastic, reactive-oxygen-driven oxidation and the staged formation of the plastisphere. The supporting evidence is graded from gene prediction to demonstration by a purified enzyme. Most outcomes prove to be physical capture or surface modification, and no study reviewed demonstrates mineralisation. We assess engineered diatoms and green algae expressing bacterial PET hydrolases and identify what field application would require: synthetic consortia, omics-based enzyme mining, photobioreactor integration, machine-learning-guided enzyme design, and standardised protocols focused on mineralisation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From selenite to selenium nanoparticle: comparative biosynthetic mechanisms across Bacillus and a roadmap for Bacillus licheniformis.","authors":"Maryam Anayat, Shiza Nawaz, Zhengrong Tao, Nida Nawaz, Shuang Liu, Fenghuan Wang, Xian Yin, Yadong Wang","doi":"10.1007/s11274-026-05332-y","DOIUrl":"https://doi.org/10.1007/s11274-026-05332-y","url":null,"abstract":"<p><p>Selenium nanoparticles (SeNPs) are increasingly studied as biologically active selenium forms with potential applications in biotechnology, medicine, agriculture, and environmental remediation. Microbial synthesis offers an alternative approach for producing SeNPs under biological conditions. Molecular mechanisms governing selenium transformation, nanoparticle assembly, and extracellular stabilisation remain incompletely understood, particularly in Bacillus licheniformis, an industrially relevant selenium-reducing bacterium. This review examines the mechanisms proposed for bacterial SeNP formation across Bacillus species, with emphasis on B. licheniformis and other microorganisms with better characterised selenium transformation. The review discusses major steps associated with SeNP formation, including selenium oxyanion uptake, intracellular reduction, redox regulation, nucleation, extracellular transport, and biomolecular stabilisation. Comparative analysis highlights recurring features, such as thiol-dependent reduction systems, oxidative stress responses, protein-associated nucleation and extracellular polymeric substances, while also showing that mechanisms identified in one bacterial species cannot be directly transferred to another. Where evidence is available, experimentally validated mechanisms are discussed separately from proposed mechanisms inferred from related organisms. SeNP formation is not considered here as a well established biosynthetic programme. In most systems, the available data are equally consistent with detoxification and general thiol redox chemistry. It also remains uncertain how far cells exert active control over particle formation. Although B. licheniformis has been shown to produce SeNPs, the molecular mechanisms underlying its selenium uptake, SeNP nucleation, export, and surface functionalisation are not fully understood. Recent progress has enabled an experimental approach to this question: a respiratory selenate reductase operon has been characterised in the genus (in B. selenatarsenatis (selenate reduced), not yet in B. licheniformis). Closed genome sequences are now available for a SeNP-producing B. licheniformis strain and for the sister taxon B. paralicheniformis, and marker-free knockout, CRISPR interference and transposon mutagenesis are established in B. licheniformis. Further targeted multi-omics and loss-of-function research will be required to elucidate the role of these candidate pathways in the formation of SeNP in B. licheniformis and their potential for use in strain improvement.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149172069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and vertical dynamics of microbial communities during open-pit coal mine topsoil stockpiling in a black soil (mollisol) region of Northeast China.","authors":"Zhe Li, Xiujie Wang, Jiawei Sun, Zhenqi Hu","doi":"10.1007/s11274-026-05328-8","DOIUrl":"https://doi.org/10.1007/s11274-026-05328-8","url":null,"abstract":"<p><p>Topsoil stockpiling is a critical yet often overlooked stage in mine reclamation. This study investigated the effects of stockpiling duration (0-3 years) and depth (0-6 m) on soil properties and microbial communities in a black soil (Mollisol) stockpile from a coal mine in Northeast China. Results showed that compared to the undisturbed farmland soil, stockpiled soils exhibited a significantly higher pH but lower soil organic carbon (SOC), total nitrogen (TN), available phosphorus (AP), and enzyme activities (invertase, urease, acid phosphatase). Vertically, SOC and TN were better preserved whereas AP and enzyme activities were significantly lower in deeper layers. High-throughput sequencing and phospholipid fatty acid analysis revealed differential responses of bacteria and fungi. Bacterial community composition transitioned from a prevalence of copiotrophic (e.g., Pseudomonadota) toward a dominance of oligotrophic taxa (e.g., Acidobacteriota) with increasing time and depth, while fungal communities showed sharper decline in biomass and greater sensitivity in composition, marked by stronger vertical differentiation, reduced network complexity, and increased competitive interactions. Functional predictions indicated suppressed bacterial metabolic potential and a shift toward saprotrophic dominance in fungi. Partial least squares path modeling revealed that stockpiling-induced pH increases and nutrient losses was closely associated with microbial shifts, with bacterial community acting as a key biological mediator directly linked to the decline in soil enzymatic function. Collectively, these findings suggest that topsoil stockpiling can lead to a dynamic process of progressive soil impoverishment, mediated by distinct microbial responses. This study provides a scientific basis for optimizing stockpiling management-such as controlling pile height and storage duration-and highlights the potential for developing microbially informed strategies to preserve soil quality and reclamation potential in black soil regions.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indole decreases the virulence of enterotoxigenic Escherichia coli.","authors":"Sofia Razzaq Meo, Christophe Noppe, Patrick De Clercq, Tom Van de Wiele, Tom Defoirdt","doi":"10.1007/s11274-026-05323-z","DOIUrl":"10.1007/s11274-026-05323-z","url":null,"abstract":"<p><p>Indole signaling is being explored as one of the promising targets for anti-virulence therapy against antibiotic-resistant pathogens. Indole has been reported to influence key virulence-related phenotypes, particularly motility and biofilm formation, in various bacterial pathogens. This study aimed to investigate the impact of indole on enterotoxigenic Escherichia coli (ETEC). The results revealed that indole decreased both swimming motility and biofilm formation in ETEC across concentrations ranging from 0 to 1000 µM, with a maximum reduction of 75% in swimming motility and 47% in biofilm formation at the highest concentration. RT-qPCR analysis revealed that motility-related genes (i.e., flagellin fliC and flagellar transcription activator flhC) were downregulated by 5-fold and 2.5-fold, respectively. Additionally, the stress response sigma factor gene rpoS and the heat-stable enterotoxin gene estP were downregulated by 2-fold and 1.7-fold, respectively. Competitive ELISA showed that indole decreased secreted heat-stable enterotoxin levels by more than 10-fold, which is consistent with the decreased estP mRNA levels. Finally, indole decreased the virulence of ETEC towards the invertebrate infection model Galleria mellonella, resulting in a 1.8-fold increased survival after 5 days. These findings indicate that indole signaling controls ETEC virulence, making it an interesting target for anti-virulence therapy.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the novel role of several Flavobacteriaceae isolates: Cultivable bacteria associated with Porolithon onkodes induce Pocillopora damicornis larval settlement and metamorphosis.","authors":"Zhiliang Xiao, Lijuan Long, Fangfang Yang","doi":"10.1007/s11274-026-05327-9","DOIUrl":"10.1007/s11274-026-05327-9","url":null,"abstract":"<p><p>Crustose coralline algae (CCA) are pivotal for coral larval settlement and metamorphosis, yet the functional roles of their associated bacteria in this critical process remain underexplored. Here, we systematically isolated 554 cultivable bacteria from the dominant CCA species Porolithon onkodes and assessed their ability to induce larval settlement and metamorphosis of Pocillopora damicornis. The isolates were dominated by Pseudomonadota (50.72%) and Bacteroidota (32.85%), with 57.40% exhibiting < 98.60% 16S rRNA gene similarity to their closest type-strain matches, suggesting that Por. onkodes may harbor substantial numbers of potentially undescribed or poorly characterized bacteria. Notably, 33 isolates significantly induced larval settlement and metamorphosis, with rates exceeding 40.00% after 96 h, mainly including 22 Flavobacteriaceae and 9 Bacillaceae isolates, substantially expanding the known taxonomic range of bacteria involved in this process. Further investigation on Flavobacteriaceae isolate B1405 (a highly active inducer) revealed that its inductive activity was tightly dependent on growth phase and biofilm age: logarithmic- or stationary-phase cells induced > 38.00% settlement, while 3-day-old biofilms significantly enhanced the settlement rate to > 60.00%. These findings fill critical gaps in CCA-bacteria-coral interactions and highlight specific cultivable isolates within Flavobacteriaceae and Bacillaceae as potential microbial candidates for further investigation in coral settlement and restoration applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149148631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic-inorganic composite inoculant promotes carbon stabilization in acidic lateritic red soil by redirecting microbial catabolism toward anabolism.","authors":"Junyu Xiang, Qian Zhang, Zicong Xiong, Zhongning Liu, Juanjuan Yin, Congyao Shi, Binhong Qin, Weiwei Yang, Chaolan Zhang","doi":"10.1007/s11274-026-05318-w","DOIUrl":"10.1007/s11274-026-05318-w","url":null,"abstract":"<p><p>Acidification, nutrient deficiency, and poor carbon pool stability are key bottlenecks restricting the sustainable development of agriculture in lateritic red soils. In this study, we isolated strains GC05 (Paracoccus communis) and GC07 (Advenella kashmirensis). A composite microbial consortium was constructed and then combined with a biochar-montmorillonite-chicken manure carrier to produce an organic-inorganic composite microbial inoculant. After applying the inoculant to acidic lateritic red soil, we investigated its mechanisms in simultaneously ameliorating acidic lateritic red soil and driving soil organic carbon stabilization. After a 30-day incubation, the inoculant ameliorated the soil environment and enhanced both nutrient availability and carbon stability. Soil pH increased from 4.86 to 5.76, the ratio of recalcitrant carbon to total organic carbon increased from 0.72 to 0.86. The inoculant promoted the enrichment of carbon assimilation-dominant functional microbial taxa. Microbial carbon use efficiency increased from 0.02 to 0.41, and the metabolic focus shifted from enzymatic depolymerization and catabolism to biomass synthesis and anabolism. Structural equation modeling further suggested that microbial biomass exerted a dominant direct effect on the stable soil carbon pool, driving the conversion of labile organic carbon into recalcitrant organic carbon via biomass synthesis and microbial necromass accumulation, which constituted an early-stage microbial carbon pump. This study provides a theoretical foundation and practical strategy for the development of bioaugmented technologies with potential for long‑term carbon sequestration in acidic lateritic red soils.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}