World journal of microbiology & biotechnology最新文献

{"title":"Mycoremediation of heavy metals by Curvularia lunata from Buckingham Canal, Neelankarai, Chennai.","authors":"S Sugitha, P Vishnu Priya, Tadela Kavya Kanishka, A Duraimurugan, M Suganthi, K Ashok Kumar, M Jayanthi, R Durgadevi, C Ramprasath, G Abirami","doi":"10.1007/s11274-024-04218-1","DOIUrl":"https://doi.org/10.1007/s11274-024-04218-1","url":null,"abstract":"<p><p>The spread and mobilization of toxic heavy metals in the environment have increased to a harmful level in recent years as a result of the fast industrialization occurring all over the world to meet the demands of a rising population. This research aims to analyze and evaluate the mycoremediation abilities of fungal strains that exhibit tolerance to heavy metals, gathered from water samples at Buckingham Canal, Neelankarai, Chennai. Water samples were examined for heavy metal analysis, and the highest toxic heavy metals, Zn, Pb, Mn, Cu, and Cr, were recorded. Three fungal strains were isolated and named EBPL1000, EBPL1001, and EBPL1002 were selected by primary screening (100 ppm) for further studies. Out of three fungal isolates, EBPL1000 grew in all five heavy metal concentrations and showed 2100 ppm as the highest Maximum Tolerance Concentration toward Lead, 2000 ppm tolerance in Zinc and Manganese, 1700 ppm in Chromium, and 1500 ppm in copper, respectively. The fungal isolate EBPL1000 was identified as Curvularia lunata with 100% percentage identity and query coverage. The Biosorption result reveals that lead is the highest biosorbed heavy metal with 79.99% at 100 ppm concentration while copper is the lowest biosorbed with 24.11% heavy metal at 500 ppm concentration. The uptake of Manganese by Curvularia lunata biomass was the highest (5.64 mg/g) of all heavy metal's uptake at 100 ppm concentration. The lowest uptake of heavy metals was copper (0.43 mg/g) at 500 ppm concentration, and the growth profile study under heavy metals stress conditions shows the order of Pb > Mn > Zn > Cr > Cu at 60 h of time intervals at 100 ppm concentration. In addition to the research, FTIR analysis and Molecular Docking studies provide credence to the idea that Curvularia lunata has high biosorption potential and uptake or removal of toxic heavy metals at low cost and in an eco-friendly way from the contaminated environment.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"1"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights into salt tolerance in Dunaliella tertiolecta involving two betaine aldehyde dehydrogenases.","authors":"Qian-Xi Zheng, Jia-Yuan Luo, Qian-Hui Wang, Hao-Hong Chen, Jian-Guo Jiang","doi":"10.1007/s11274-024-04217-2","DOIUrl":"https://doi.org/10.1007/s11274-024-04217-2","url":null,"abstract":"<p><p>Understanding salt tolerance mechanisms is crucial for addressing the global challenge of soil salinization and advancing sustainable agricultural practices. Dunaliella tertiolecta, thriving in up to 4.5 M NaCl, is a model for studying salt tolerance mechanisms. Two betaine aldehyde dehydrogenase (BADH) genes were identified in D. tertiolecta, namely DtBADH1 and DtBADH2. The phylogenetic analysis revealed that DtBADH1 had similarity to Pseudomonas aeruginosa BADH, while DtBADH2 has high homology to aldehyde dehydrogenase from Chlorella sorokiniana. The 3D models of DtBADH1 and DtBADH2 docking with betaine aldehyde were performed to further validate their binding site, interactions binding the protein and its substrate as well as the conserved amino acids responsible for enzyme activity. We also conducted RNA interference of DtBADH1 and DtBADH2 in D. tertiolecta. Compared to the wild type D. tertiolecta, both BADH-RNAi D. tertiolecta had fewer cell numbers and relatively lower glycine betaine content under high salinity. The findings suggest that both DtBADH1 and DtBADH2 play a crucial role in betaine synthesis, indicating their potential involvement in salt tolerance mechanisms at the molecular level. Additionally, these results highlight D. tertiolecta as a promising candidate for identifying salt stress-responsive genes, which could be utilized for engineering algae or crops to enhance their ability to withstand salinity stress.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"8"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effects of berberine on fungal growth, biofilm formation, virulence, and drug resistance as an antifungal drug and adjuvant with prospects for future applications.","authors":"Junping Ding, Zhong Yan, Liang Peng, Jing Li, Fuzhou Yang, Dongming Zheng","doi":"10.1007/s11274-024-04223-4","DOIUrl":"10.1007/s11274-024-04223-4","url":null,"abstract":"<p><p>Berberine (BBR), an isoquinoline alkaloid found in medicinal plants such as Coptidis rhizoma, Berberis sp., and Hydrastis canadensis, is a distinctive compound known for its dual ability to exhibit broad-spectrum antifungal activity while offering beneficial effects to the host. These attributes make it a highly valuable candidate for antifungal therapy and as an antibiotic adjuvant. This review provides a comprehensive evaluation of BBR's antifungal properties, focusing on its in vitro and in vivo activity, underlying mechanisms, and its influence on fungal pathogenicity, including virulence, biofilm formation, and resistance. Additionally, the antifungal potential of BBR extracts, derivatives, and nanoformulations is examined in detail. BBR demonstrates fungicidal effects through multiple mechanisms. It targets critical fungal components such as mitochondria, cell membranes, and cell walls, while also inhibiting enzymatic activity and transcription processes. Furthermore, it suppresses the expression of virulence factors, effectively diminishing fungal pathogenicity. Beyond its direct antifungal activity, BBR exerts beneficial effects on the host by modulating gut microbiota, thereby bolstering host defenses against fungal infections and reducing potential adverse effects. BBR's interaction with conventional antifungal drugs presents a unique complexity, particularly in the context of resistance mechanisms. When used in combination therapies, conventional antifungal drugs enhance the intracellular accumulation of BBR, thereby amplifying its antifungal potency as the primary active agent. These synergistic effects position BBR as a promising candidate for combination strategies, especially in addressing drug-resistant fungal infections and persistent biofilms. As antifungal resistance and biofilm-associated infections continue to rise, the multifaceted properties of BBR and its advanced formulations highlight their significant therapeutic potential. However, the scarcity of robust in vivo and clinical studies limits a full understanding of its efficacy and safety profile. To bridge this gap, future investigations should prioritize well-designed in vivo and clinical trials to thoroughly evaluate the therapeutic effectiveness and safety of BBR in diverse clinical settings. This approach could pave the way for its broader application in combating fungal infections.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"5"},"PeriodicalIF":4.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Total carbohydrate consumption through co-fermentation of agroindustrial waste: use of wild-type bacterial isolates specialized in the conversion of C-5 sugars to high levels of lactic acid with concomitant metabolization of toxic compounds.","authors":"Sheila Montipó, Elisa Bellan Menegussi, Marli Camassola, Ola Wallberg, Mats Galbe","doi":"10.1007/s11274-024-04216-3","DOIUrl":"10.1007/s11274-024-04216-3","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"406"},"PeriodicalIF":4.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term responses of identified soil beneficial-bacteria to the insecticide fipronil: toxicological impacts.","authors":"Mohammad Shahid, Udai B Singh, Mohammad Abul Farah, Khalid Mashay Al-Anazi","doi":"10.1007/s11274-024-04203-8","DOIUrl":"10.1007/s11274-024-04203-8","url":null,"abstract":"<p><p>Pesticides including insecticides are often applied to prevent distortion posed by plant insect pests. However, the application of these chemicals detrimentally affected the non-target organisms including soil biota. Fipronil (FIP), a broad-spectrum insecticide, is extensively used to control pests across the globe. The frequent usage calls for attention regarding risk assessment of undesirable effects on non-target microorganisms. Here, laboratory-based experiments were conducted to assess the effect of FIP on plant-beneficial bacteria (PBB); Rhizobium leguminosarum (Acc. No. PQ578652), Azotobacter salinestris (Acc. No. PQ578649) and Serratia marcescens (Acc. No. PQ578651). PBB synthesized growth regulating substances were negatively affected by increasing fipronil concentrations. For instance, at 100 µg FIPmL^-1, a decrease in indole-3-acetic acid (IAA) synthesis by bacterial strains followed the order: A. salinestris (95.6%) S. marcescens (91.6%) > R. leguminosarum (87%). Also, exposure of bacteria cells to FIP hindered the growth and morphology of PBB observed as distortion, cracking, and aberrant structure under scanning electron microscopy (SEM). Moreover, FIP-treated and propidium iodide (PI)-stained bacterial cells displayed an insecticide dose-dependent increase in cellular permeability as observed under a confocal laser microscope (CLSM). Colony counts (log₁₀ CFU mL^-1) and growth of A. salinestris was completely inhibited at 150 µg FIPmL^-1. The surface adhering ability (biofilm formation) of PBB was also disrupted/inhibited in a FIP dose-related manner. The respiration loss due to FIP was coupled with a reduction in population size. Fipronil at 150 µgmL^-1 decreased cellular respiration in A. salinestris (72%) S. marcescens (53%) and R. leguminosarum (85%). Additionally, biomarker enzymes; lactate dehydrogenase (LDH), lipid peroxidation (LPO), and oxidative stress (catalase; CAT) induced by FIP represented significant (p ≤ 0.05) toxicity towards PBB strains. Conclusively, fipronil suggests a toxic effect that emphasizes their careful monitoring in soils before application and their optimum addition in the soil-plant system. It is high time to prepare both target-specific and slow-released agrochemical formulation for crop protection with concurrent safeguarding of soils.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"403"},"PeriodicalIF":4.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological properties of Bacillus amylolyquefaciens B65 isolated from an artisanal tannery.","authors":"Inés María Virgili Alemán, Gabriela Petroselli, Rosa Erra-Balsells, Mirta Daz, Marcela Carina Audisio","doi":"10.1007/s11274-024-04215-4","DOIUrl":"10.1007/s11274-024-04215-4","url":null,"abstract":"<p><p>Leather industry is traditionally characterized by the use of large amounts of chemical agents, some of which are toxic to human health and the environment. However, during the last years, many efforts have been made with the aim of successfully implement enzymes as agents for different leather production stages. The lipopeptides produced by the Bacillus spp. genus have excellent surfactants and antibacterial properties and may collaborate in the soaking stage of leather processing as well as in leather preservation. Moreover, Bacillus sp. proteases and lipopeptides can be co-produced in one culture medium, saving the production costs. In the present work, a screening of enzymatic activities was performed on 11 strains of the Bacillus sp. genus that have been isolated from samples of an artisan tannery from Salta, Argentina. In particular, the ability of B. amyloliquefaciens B65 to degrade α-type (nails, hair, wool) and β-type (feathers) keratin was demonstrated by scanning electron microscopy (SEM). The co-production of proteases, keratinases, glycosidases, and lipopeptides of this strain was conducted at 37 °C in mineral media supplemented with chicken feathers. In these nutrient-deficient media, the strain secreted amylases, pectinases, proteases, keratinases, and collagenases. A MALDI-TOF study also revealed that the strains secreted homologues of kurstakins, iturins, surfactins, and fengycines lipopeptides families. Therefore, B. amyloliquefaciens B65 presents great industrial potential applications, not only for tanneries but also for other industries such as pharmaceuticals, food, textiles, and detergents, among others.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"405"},"PeriodicalIF":4.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iminoglutamic succinic acid: assessment of the degree of biodegradation and toxicity of half-decomposition products.","authors":"Polina Aleksandrovna Kuryntseva, Nataliya Andreevna Pronovich, Yulia Olegovna Bukarinova, Darya Lvovna Khlebova, Alina Rinatovna Kamalova, Aliia Nailevna Khamieva, Marsel Maratovich Khamiev, Polina Yurevna Galitskaya, Svetlana Yurevna Selivanovskaya","doi":"10.1007/s11274-024-04207-4","DOIUrl":"10.1007/s11274-024-04207-4","url":null,"abstract":"<p><p>Chelating agents used in agriculture and land reclamation practices must exhibit the capacity to firmly bind metal ions while also rapidly decomposing into non-toxic compounds due to their introduction into the environment in substantial quantities. It is therefore crucial to identify an alternative to EDTA, a prevalent chelating agent known for its low biodegradability and relatively high toxicity. This study focuses on assessing the degree of biodegradation of iminoglutamic succinic acid (IGSA) and the toxicity of its half-decomposition products. For this purpose, two bacterial and fungal isolates capable of decomposing IGSA were isolated and characterized, identified as Ralstonia pickettii and Fusarium foetens, respectively. The results of a 28-day experiment demonstrated that Ralstonia pickettii was capable of decomposing IGSA by 18.0% according to the manometric test and by 24.5% based on the analysis of decomposition products via HPLC. In comparison, Fusarium foetens exhibited a more effective biodegradation capacity, with rates of 20.3% and 32.5%, respectively. The half-decomposition products of IGSA were characterized by low ecotoxicity levels (LID10) concerning Paramecium caudatum (11.2-13.2 g/l) and Ceriodaphnia affinis (3.6-8.9 g/l). However, a stimulating effect was observed on microalgae, with growth stimulation ranging from 63.65-96.60%.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"404"},"PeriodicalIF":4.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating multidrug-resistant (MDR) Staphylococcus aureus infection using terpene and its derivative.","authors":"Nor Hawani Salikin, Lee Chee Keong, Wan-Atirah Azemin, Noraini Philip, Nurhaida Yusuf, Siti Aisyah Daud, Syarifah Ab Rashid","doi":"10.1007/s11274-024-04190-w","DOIUrl":"10.1007/s11274-024-04190-w","url":null,"abstract":"<p><p>Multidrug-resistant (MDR) Staphylococcus aureus represents a major global health issue resulting in a wide range of debilitating infections and fatalities. The slow progression of new antibiotics, limited choices for treatment, and scarcity of new drug approvals create immense obstacles in new drug line development. S. aureus poses a significant public health risk, due to the emergence of methicillin-resistant (MRSA) and vancomycin-resistant strains (VRSA), necessitating novel antibiotics for effective control management. Current studies are delving into the terpenes' potential as an antimicrobial agent, indicating positive prospects as promising substitutes or complementary to conventional antibiotics. Concurrent reactions of terpenes with conventional antibiotics create synergistic effects that significantly enhance antibiotic efficacy. Accumulated evidence has shown that while efflux pump (e.g., NorA, TetK, and MepA) is revealed as an essential defense of S. aureus against antibiotics, terpene and its derivative act as its potent inhibitor, suggesting the promising potential of terpenes in combating those infectious pathogens. Furthermore, pronounced cell membrane disruptive activity and antibiofilm properties by terpenes have been exerted, signifying their significance as promising prevention against microbial pathogenesis and antimicrobial resistance. This review provides an overview of the potential of terpenes and their derivatives in combating S. aureus infections, highlighting their potential mechanisms of action (MOA), synergistic effects with conventional antibiotics, and challenges in clinical translation. The unique properties of terpenes offer an opportunity for their use in developing an exceptional defense strategy against antibiotic-resistant S. aureus.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"402"},"PeriodicalIF":4.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A label-free DNAzyme-based colorimetric sensor for the detection of Leptospira interrogans.","authors":"Elangovan Saranya, Archana Vishwakarma, Kiran K Mandrekar, Kakithakara Vajravelu Leela, Mohandass Ramya","doi":"10.1007/s11274-024-04210-9","DOIUrl":"10.1007/s11274-024-04210-9","url":null,"abstract":"<p><p>Leptospirosis is a neglected zoonosis caused by a pathogenic spirochete Leptospira. Diagnosis of leptospirosis in the early stage is difficult and can be easily confused with other infections. The existing detection methods are considered chronophagous and labor-intensive. Leptospira survives in the kidney tubules of reservoir animals such as rodents and shed into the environment through their urine. In this study, we developed an Aptamer-DNAzyme-based biosensor for detecting pathogenic Leptospira in environmental water samples. The cell-specific aptamer with an extensive affinity binds to the cell surface proteins to detect the Leptospira interrogans. The DNAzyme that mimics as a peroxidase enzyme, acts as a transducing agent in the colorimetric reaction positively conditioned by the presence of L. interrogans. The Leptospira-specific aptamer coupled with DNAzyme is coated onto carbon nanotubes, to provide a cost-effective nanomaterial-based detection platform. L. interrogans contamination in the samples is detected with a color change of a peroxidase substrate, ABTS. The dissociation constant of the aptazyme was found to be 356.6 nM. The aptazyme system was able to detect up to 119 CFU/mL of L. interrogans exhibiting a high range of selectivity towards the pathogenic spirochete. This simple detection methodology makes the system promising for the environmental monitoring of L. interrogans.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"401"},"PeriodicalIF":4.0,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial degradation of polyethylene polymer: current paradigms, challenges, and future innovations.","authors":"Babbiker Mohammed Taher Gorish, Waha Ismail Yahia Abdelmula, Sivasamy Sethupathy, Mudasir A Dar, Mohd Shahnawaz, Daochen Zhu","doi":"10.1007/s11274-024-04211-8","DOIUrl":"10.1007/s11274-024-04211-8","url":null,"abstract":"<p><p>Polyethylene (PE) is the second most commonly used plastic worldwide, mainly used to produce single-use items such as bags and bottles. Its significant resistance to natural biodegradation results in the accumulation of PE in landfills, leading to various ecological and toxicological consequences. Despite extensive research on the microbial degradation of PE, achieving complete biodegradation remains a challenge. Comparing experimental outcomes is complicated by the diverse array of microbes involved in PE biodegradation, variations in culture conditions, and differences in assessment tools. This review discusses the critical hurdles in PE biodegradation experiments, including the chemical complexity of PE substrates and the challenges of isolating effective microbes and forming stable consortia. The review also delves into the difficulties in accurately assessing microbial metabolic activity and understanding the biochemical pathways involved in PE degradation. Furthermore, it addresses the pressing issues of metabolic byproducts, slow degradation rates, scalability concerns, and the challenges in measuring biodegradation levels effectively. In addition to outlining the technical challenges associated with PE experiments, this review offers recommendations for future research directions to enhance PE biodegradation outcomes. Overcoming these challenges and implementing the proposed future strategies will improve the reliability, comparability, and practicality of current PE biodegradation experiments, ultimately contributing to better comprehension and management of PE waste in the environment.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"399"},"PeriodicalIF":4.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}