World journal of microbiology & biotechnology最新文献

{"title":"The antibacterial activity, action mechanisms and prospects of Baicalein as an antibacterial adjuvant.","authors":"Liuqing Mu, Kaiyan Xie, Rong Chen, Jiang Cao, Yang Shu, Xuebin Huang","doi":"10.1007/s11274-025-04508-2","DOIUrl":"https://doi.org/10.1007/s11274-025-04508-2","url":null,"abstract":"<p><p>Given the rising issue of antimicrobial resistance and the slow progress in the development of novel antibiotics, the identification of antimicrobial agents from ethnopharmacological plants has become a promising strategy. Baicalein is a flavonoid compound derived from medicinal plants with a wide range of pharmacological activities. It exhibits broad-spectrum antimicrobial activity against various clinically relevant bacteria and fungi in a dose-dependent manner. Moreover, baicalein suppresses the development of antimicrobial resistance, enhances the efficacy of conventional antibiotics, and converts their bacteriostatic effects into bactericidal activity. This highlights its potential as both an antimicrobial agent and an antibiotic adjuvant. This review systematically summarizes the antimicrobial properties of baicalein, both as a monotherapy and in combination therapy, focusing on its effects on planktonic and biofilm cells, as well as its beneficial actions in vivo infections. Moreover, we provide an in-depth analysis of the potential molecular targets of baicalein, including the cell membrane and cell wall, core metabolic processes, resistance mechanisms, and quorum sensing systems. We also discuss the limitations of current research and outline future research directions, emphasizing the need for high-quality studies to comprehensively evaluate the clinical efficacy and safety of baicalein in infection treatment. This review aims to offer new insights and recommendations for its future clinical translation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"304"},"PeriodicalIF":4.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyroligneous acid as a multifunctional biostimulant enhances microalgal growth and soil beneficial metabolites for sustainable agriculture.","authors":"Sudharsanam Abinandan, Praveen Kuppan, Kadiyala Venkateswarlu, Kannappar Mukunthan, Mallavarapu Megharaj","doi":"10.1007/s11274-025-04514-4","DOIUrl":"10.1007/s11274-025-04514-4","url":null,"abstract":"<p><p>Microalgae are recognized as eco-functional inoculants that improve soil quality and stimulate plant productivity. This study explored the potential of pyroligneous acid (PA), a low-cost byproduct of biomass pyrolysis, as a biostimulant enhancing microalgal growth and metabolite production. We investigated the effects of PA on growth, and synthesis of indole-3-acetic acid (IAA) and extracellular polymeric substances (EPS) in two microalgal strains, Desmodesmus sp. MAS1 and Chlorella sp., when cultivated with PA in presence or absence of light. Preliminary studies showed that 0.01% (v/v) of PA enhanced growth of the microalgae, and its increased concentrations were inhibitory. Growth rate of Chlorella sp. with PA was 0.444 d^‒1 in a photobioreactor compared to 0.465 d^‒1 without PA, while the corresponding values for strain MAS1 were 0.372 d^‒1 and 0.334 d^‒1, respectively. Biomass production for Chlorella sp. and strain MAS1 when grown in presence of PA was 0.80 and 1.54 g L^‒1, respectively, while the biomass of cultures grown in the absence of PA was 1.13 and 1.20 g L^‒1, respectively. Chlorophyll content increased by 27% (P < 0.05) in Chlorella sp. and 17% (P < 0.05) in strain MAS1 in presence of PA. For Chlorella sp., IAA production in presence and absence of PA was 4.40 and 2.40 µg g^‒1, respectively, while it was 6.0 and 2.0 µg g^‒1, respectively, for strain MAS1. EPS yield increased by 2.0 mg g^‒1 with PA in Chlorella sp., and the increase was 8.0 mg g^‒1 in case of strain MAS1. Chlorella sp. exhibited higher chlorophyll content, enhanced IAA and EPS production but there was no increase in biomass. This suggests that PA can improve specific metabolic activities in both the microalgal strains, possibly contributing to soil health by promoting soil structure through increased EPS and IAA yield. Subsequent soil-based microcosm experiments confirmed that microalgae together with PA significantly increased soil EPS, IAA, dehydrogenase activity (DHA), and chlorophyll a content. The increase in DHA was 2.4-fold in soils that received PA and strain MAS1, while EPS production was 1.6 times higher. Chlorophyll a content was maximum in PA-amended soils inoculated with Chlorella sp., reaching a significant 6.5-fold increase that remained thereafter. These results highlight the dual role of PA in stimulating microalgal metabolic activity and enhancing soil microbial function, positioning it as a multifunctional biostimulant for sustainable agriculture.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"306"},"PeriodicalIF":4.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cadmium removal and indole acetic acid production by ureolytic bacteria isolated from rhizosphere soils.","authors":"Carlos A Adarme-Duran, Elianna Castillo, Pedro F B Brandão","doi":"10.1007/s11274-025-04482-9","DOIUrl":"10.1007/s11274-025-04482-9","url":null,"abstract":"<p><p>This work reports the isolation of 54 ureolytic bacteria with microbiologically induced carbonate precipitation (MICP) activity from Theobroma cacao L. rhizosphere soils with Cd concentration ranging from 0.49 to 21.29 mg kg^- 1. Analysis of the 16 S rRNA gene showed the isolates belong to the genera Serratia, Pseudomonas, Comamonas, Klebsiella, Bacillus, Citrobacter, Flavobacterium, Delftia, and Stenotrophomonas. They showed ureolytic activity between 0.31 and 1.01 µmol NH₄⁺ mL^- 1 h^- 1 and produced the phytohormone indole acetic acid (IAA). Interestingly, Cd was observed to increase ureolytic activity and did not affect the IAA production of various isolates. Cadmium removal in solution ranged from 4.4 to 87.0% after 48 h. Strains Serratia sp. 89a, Klebsiella sp. 76 h, and Stenotrophomonas sp. 67w exhibited the best performance (> 80%). Cadmium removal through MICP by the genera Delftia, Flavobacterium, and Klebsiella is reported for the first time. Results suggest the isolated bacteria have the potential for Cd bioremediation and could be used to alleviate metal-induced stress through IAA production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"302"},"PeriodicalIF":4.2,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144800419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"S292L mutation in Rv1258c efflux pump drives pyrazinamide efflux and a novel inhibitor designed for co-therapy to improve MDR-TB treatment outcomes.","authors":"Garima Singh, Shriti Shreya, Saurabh Yadav, Yusuf Akhter","doi":"10.1007/s11274-025-04510-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04510-8","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"300"},"PeriodicalIF":4.2,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144795672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery and characterization of bioactive compounds from Limnophila aromatica: nevadensin and related flavonoids as potent antimicrobial agents.","authors":"Thanaporn Maswanna, Cherdsak Maneeruttanarungroj","doi":"10.1007/s11274-025-04523-3","DOIUrl":"https://doi.org/10.1007/s11274-025-04523-3","url":null,"abstract":"<p><p>Limnophila aromatica, a traditional medicinal plant, has been previously reported to possess notable antimicrobial properties. However, the specific bioactive constituents responsible for this activity remain largely unidentified. This study aimed to isolate, identify, and evaluate the antibacterial potential of compounds from a 100% ethanolic extract of L. aromatica. The crude ethanolic extract exhibited the highest antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) and was subsequently subjected to Diaion HP-20 column chromatography, followed by preparative HPLC. Seven major peaks were identified using UV-Vis spectra, LC-QTOF-MS, and NMR analyses. The antibacterial efficacy of these isolated compounds was assessed using disc diffusion and broth microdilution assays against Bacillus subtilis, S. aureus, MRSA, Escherichia coli, and Pseudomonas aeruginosa. The isolated compounds were identified as norethindrone acetate, isothymusin, nevadensin, gardenin B, 5,3'-dihydroxy-7,8,2'-trimethoxyisoflavone, jasmolin II, and oleanolic acid. Nevadensin, the predominant compound (79.55%), demonstrated potent bactericidal activity against B. subtilis, S. aureus, and MRSA. Jasmolin II and gardenin B also exhibited promising antibacterial effects. Although the disc diffusion assay was limited by compound diffusion, the broth microdilution method confirmed significant MIC values, ranging from 0.59 to 2.86 mg/ml while the MBC values ranged from 0.59 to 12.72 mg/ml. This study highlights nevadensin and other flavonoids from L. aromatica as potential candidates for the development of alternative antibacterial therapies, particularly against drug-resistant Gram-positive bacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"301"},"PeriodicalIF":4.2,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144795671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and application of an efficient diesel degrading bacterial consortium for oily wastewater bioremediation.","authors":"Ning Zhang, Yajun Li, Zhenzhen Jiang, Hanghai Zhou, Ming Zhou, Ruichang Zhang, Xing Ren, Chunfang Zhang, Xuefeng Wei","doi":"10.1007/s11274-025-04520-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04520-6","url":null,"abstract":"<p><p>The treatment of oil-contaminated wastewater represents a significant environmental challenge. In this study, five highly efficient diesel-degrading bacterial strains were successfully isolated from oily wastewater, which was designated as Pseudomonas sp. ZC1, Vibrio sp. ZL2, Acinetobacter sp. ZY3, Citrobacter sp. GO5, and Enterobacter cloacae GM6. To construct an efficient bacterial consortium based on these five strains, the optimized strain combination of 26 different consortia composed of two, three, four, and five bacterial strains was conducted. The results suggested that a consortium comprising three strains (ZL2, ZY3, and GM6) showed the highest diesel degradation efficiency of 89.66% on day 3, while other strain combinations exhibited lower degradation rates and tended to require more time to achieve comparable efficiency. The orthogonal experiments further determined the optimal inoculation ratios of 1.0%, 0.5%, and 1.5% for strains ZL2, ZY3, and GM6 improved diesel degradation efficiency to 93.65% by day 3. The application of this consortium in the oily wastewater bioremediation confirmed its degrading capacity for n-alkanes (C8-C40) and polycyclic aromatic hydrocarbons (PAHs). This study highlights the excellent performance of the engineered bacterial consortium in the bioremediation of petroleum-contaminated wastewater, demonstrating its potential for scaling up to pilot and full-scale applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"299"},"PeriodicalIF":4.2,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144790195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifidobacteria in antibiotic-associated dysbiosis: restoring balance in the gut microbiome.","authors":"Rajashree Jena, Namita Ashish Singh, Nafees Ahmed, Prasanta Kumar Choudhury","doi":"10.1007/s11274-025-04517-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04517-1","url":null,"abstract":"<p><p>Antibiotic-associated dysbiosis disrupts the gut's microbial balance, leading to reduced diversity, overgrowth of antibiotic-resistant strains, and compromised gut homeostasis. This can result in inflammation, increased intestinal permeability, impaired immunity, and heightened susceptibility to infections. In this context, probiotics have been highlighted as a promising remedy in alleviating this antibiotic-induced gut microbiome aberrations with subsequent decrease of the detrimental effects. Bifidobacteria, a prominent bacterial group with promising probiotic attributes, have shown effectiveness in restoring the gut microbiome by strong adherence to the colon's mucosal lining and enhancing the immune response through increased anti-inflammatory cytokines. They also play a crucial role as key producers of acetic acid, which supports butyric acid-producing bacteria essential for colonocyte health during dysbiosis. The synergistic use of bifidobacteria with other probiotic species or prebiotic substrates has further enhanced their survival, colonization capacity, and overall impact on gut microbial restoration. Advanced metagenomic analyses have begun to reveal strain-specific functions, paving the way for personalized probiotic therapies tailored to an individual's unique microbiome profile. Despite encouraging progress, critical research gaps persist, particularly regarding strain-specific efficacy, formulation stability, long-term outcomes, underlying mechanisms, systemic interactions, and the distinct and specific role of bifidobacteria. Addressing these gaps through targeted clinical investigations is essential to fully harness their therapeutic potential and develop optimized strategies for restoring the microbial balance in the gut microbiome.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"297"},"PeriodicalIF":4.2,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144790194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promiscuity of lanthipeptide enzymes: new challenges and applications.","authors":"Carlos García-Ausencio, Fernando Guzmán-Chávez, Romina Rodríguez-Sanoja, Sergio Sánchez","doi":"10.1007/s11274-025-04505-5","DOIUrl":"10.1007/s11274-025-04505-5","url":null,"abstract":"<p><p>Lanthipeptides are a group of peptides synthesized by ribosomes that undergo post-translational modifications and have significant potential for medical and biotechnological applications. Various bacterial strains produce these peptides, and their synthesis involves the structural modification of precursor compounds through specialized enzymes present within a biosynthetic gene cluster (BGC) of the producing organisms. These enzymes are particularly notable for their capacity to modify non-cognate substrates, allowing for the installation of lanthionine rings on precursor peptides and enabling further modifications, such as methylation, reduction, and oxidation, to enhance the biological properties of specific peptides. The inherent flexibility of lanthipeptide enzymes-an important characteristic of this class of proteins-can be utilized to create peptides with improved bioactive and physicochemical properties. This review synthesizes recent advances in the application of promiscuous enzymes for the synthesis of bioactive peptides, emphasizing the diverse classes identified to date.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"298"},"PeriodicalIF":4.2,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144790196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold nanocluster-based biosensing for rapid detection of carbapenem-resistant organisms in bloodstream infections.","authors":"Rahul Harikumar Lathakumari, Leela Kakithakara Vajravelu, Poornima Baskar Vimala, Vishnu Priya Panneerselvam, Dakshina M Nair, Jayaprakash Thulukanam, Ashwin Kumar Narasimhan","doi":"10.1007/s11274-025-04490-9","DOIUrl":"https://doi.org/10.1007/s11274-025-04490-9","url":null,"abstract":"<p><p>Carbapenem resistance in Gram-negative bacteria poses a critical global health challenge, particularly in the context of bloodstream infections (BSIs) where treatment options are severely limited and diagnostic delays can be fatal. Existing methods for detecting carbapenem resistant organisms (CROs) often lack the speed, sensitivity, and specificity required for timely clinical intervention, and currently, no validated approach exists for direct detection from blood samples. In this study, we introduce a novel diagnostic strategy utilizing bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs), which exhibit a strong fluorescence emission and average size of 2.9 nm. This method enabled rapid detection of carbapenem resistance among 400 Gram-negative clinical isolates, with 97 confirmed resistant by both phenotypic and genotypic analyses. Real-time PCR revealed NDM and VIM as the most common resistance genes, followed by OXA-48, IMP, and KPC. The BSA-AuNC assay detected as few as 10 CFU/mL in cultured isolates within 1.5 h, achieving 100% positive and negative predictive values. Remarkably, when applied directly to centrifuged blood samples, the assay maintained a high sensitivity of 95.8% with a detection limit of 1000 CFU/mL in under 2 h, outperforming the Carba NP test, which showed only 85.56% sensitivity. These findings highlight the potential of BSA-AuNCs as a rapid, sensitive, and culture-independent tool for early identification of carbapenem-resistant organisms in BSIs, offering critical support for timely and effective clinical decision-making.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"294"},"PeriodicalIF":4.2,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress genomics of the toxigenic cyanobacteria: environmental and biotechnological perspectives.","authors":"Amit Srivastava, Eetika Chot, Vipin Gupta, Nirjara Singhvi, Pratyoosh Shukla","doi":"10.1007/s11274-025-04509-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04509-1","url":null,"abstract":"<p><p>The genomic investigation of toxigenic cyanobacteria reveals unique features of potential genes, proteins, and genomic regions associated with varied functions critical for their survival and stress tolerance. Cyanobacteria are prevalent photoautotrophic microorganisms forming harmful blooms in aquatic environments, with significant public health and ecological implications. Despite the availability of complete genome sequences, the stress genomics of these harmful cyanobacteria remains understudied. This review highlights the genomic \"arsenal\" of these resilient species, emphasizing their stress adaptation mechanisms and potential vulnerabilities. Understanding this molecular basis is essential for developing targeted strategies to mitigate their impact. The insights gained from the genomic analysis could be leveraged to express unexploited stress-related genes for enhanced stress tolerance in industrial applications. Additionally, the review underscores the importance of redirecting research focus towards the functional genomics of bloom-forming strains to uncover novel pathways and strategies for their selective eradication and to improve the productivity of beneficial cyanobacterial strains under fluctuating environmental conditions. Finally, this review is an effort towards creating an important genomic resource for such toxic cyanobacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"295"},"PeriodicalIF":4.2,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}