World journal of microbiology & biotechnology最新文献

{"title":"Advances in metabolic engineering and fermentation for 3-hydroxypropionic acid biosynthesis: a comprehensive review.","authors":"Harshvardhan Joshi, Jasmine Isar, Vidhya Rangaswamy, Anu Raghunathan","doi":"10.1007/s11274-025-04554-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04554-w","url":null,"abstract":"<p><p>The grand challenge in biobased Manufacturing Lies in achieving the sustainable, economically competitive conversion of renewable biomass into high-value Chemicals capable of replacing fossil-derived products. Among these, 3-hydroxypropionic acid (3-HP) has emerged as a top-tier target-an exceptionally versatile platform molecule. It finds applications in the synthesis of acrylic acid, 1,3-propanediol, and other derivatives, positioning it as a potential cornerstone for bio-based plastics. This review consolidates the latest breakthroughs in microbial 3-HP production, encompassing advanced strain engineering, pathway rewiring, cofactor optimization, metabolic modeling, and flux balance analysis. We critically examine strategies to overcome inherent metabolic and physiological constraints, including byproduct suppression, redox balancing, and tolerance engineering. Emerging approaches-such as dynamic regulation of metabolic flux, control of cell morphology and density, and integration of co-production pathways-are highlighted for their capacity to boost yields and process robustness. Additionally, we address the fermentation process innovations targeting enhanced productivity, substrate efficiency, minimal nutrient input, and industrially relevant titres. Collectively, these insights Chart a clear path toward the scalable, sustainable biomanufacturer of 3-HP.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"352"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ITS metabarcoding of filamentous fungi in traditional biodegradation of Carapa Guianensis seeds from the Brazilian Amazon.","authors":"Mariana Barbieri, Luana Lessa R Santos, Ana C Bahia, Susana Frases","doi":"10.1007/s11274-025-04581-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04581-7","url":null,"abstract":"<p><p>The relationship between microbial communities and traditional processing techniques represents a crucial intersection of cultural heritage and biological diversity. To provide the first detailed molecular characterization of this process for andiroba (Carapa guianensis) seeds, this study employed ITS1 and ITS2 metabarcoding with two primary objectives: (1) to characterize and compare the fungal communities across three distinct management systems (agroforestry, consortium, and native forest) in the Brazilian Amazon, and (2) to identify core fungal taxa and their ecological interactions. Our analysis characterized a diverse mycobiome dominated by the phylum Ascomycota, with the generaArthrinium, Aspergillus, Penicillium, andTalaromyces being most prevalent. In our dataset, ITS1 recovered more ASVs (215) than ITS2 (117), and we observed the unexpected finding of lower fungal diversity in the agroforestry system compared to the consortium system. These findings bridge traditional ecological knowledge with modern biotechnology, validating an ancestral practice while uncovering a rich fungal consortium with significant potential for future industrial applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"348"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal potential of hydrothermal liquefaction wastewater in plant protection.","authors":"Emre Demirer Durak","doi":"10.1007/s11274-025-04573-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04573-7","url":null,"abstract":"<p><p>This study investigates the antifungal potential of hydrothermal liquefaction (HTL) wastewater against Verticillium dahliae and its effects on the growth of pepper plants (Capsicum annuum L.). The HTL process generates wastewater containing various antimicrobial compounds, which can offer a sustainable alternative for plant protection. In this research, the biological control agent Trichoderma virens and HTL wastewater were applied both individually and in combination to assess their impact on plant growth and pathogen suppression. The results demonstrated that specific HTL wastewater treatments significantly enhanced root and shoot growth, biomass, and chlorophyll content in pepper plants. Plant growth observed in pathogen-free conditions may be related to the stimulation of plant physiology by biologically active components contained in wastewater, indicating a biostimulatory effect. Notably, the 4th wastewater mixture (wm) exhibited the highest efficacy, promoting plant development and mitigating the negative effects of V. dahliae. The combination of T. virens and wastewater also showed synergistic effects, reducing disease severity by up to 64% and improving plant biomass and structural parameters. Statistical analysis revealed significant differences among treatments, highlighting the potential of HTL wastewater as a natural and sustainable strategy for managing soil-borne pathogens. These findings suggest that integrating HTL wastewater with biocontrol agents could offer a promising approach to sustainable agriculture.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"349"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of Mithi river water quality: pollution assessment, and lead bioremediation by indigenous bacteria.","authors":"Pooja Sevak, Bhupendra Pushkar, Shyamalava Mazumdar","doi":"10.1007/s11274-025-04589-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04589-z","url":null,"abstract":"<p><p>Environmental pollution is a biggest challenge faced by whole world at current time. The natural resources are victims of pollution and contamination of natural resources with heavy metals such as Pb. Pb is a highly toxic and frequent pollutant all around the world. In present study, Mithi River, a highly polluted urban water body was physico-chemically analyzed at four different sites along its course and their association was statistically analysed. Further, Pb bioremediating bacteria were isolated from Mithi river water and analysed for their Pb remediation potential. The findings revealed consistently low water quality across all sites, indicating high pollution levels, particularly in the lower stretch of river. Mithi River exhibited a substantial total heterotrophic bacteria (THB) (Taximan- 2.89 × 10⁹ and BKC- 2.13 × 10⁹) and a prevalence of lead-resistant bacteria (Taximan- 3.16 × 10⁸ and BKC 2.24 × 10⁷). A total 21 Pb resistant bacteria were isolated and gram negative coccobacilli were the most dominant. Notably, P. mirabilis demonstrated ability to bioremediate up to 100% of lead within 48 h. The SEM-EDX analysis illustrated biosorption of Pb on cell surface. Furthermore, P. mirabilis secretes significant amount of extracellular components in Pb concentration dependent manner. The study demonstrated that P. mirabilis exhibits tolerance to a wide range of environmental conditions, including variations in pH, temperature, salinity, and resistance to various toxic pollutants like heavy metals and organic contaminants. This broad tolerance spectrum to environmental conditions, combined with its strong bioremediation potential, highlights P. mirabilis as a promising candidate for lead bioremediation. Though, real-time application of bioremediation process is still lagging behind and needs immediate attention.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"350"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic and Non-Enzymatic decolorization of synthetic dyes using spent mushroom substrate.","authors":"Wenjing Xiao, Ya Ran, Xinyu Guo, Yujun Dai, Zhilin Zhang, Jiashu Liu, Changchun Li","doi":"10.1007/s11274-025-04586-2","DOIUrl":"https://doi.org/10.1007/s11274-025-04586-2","url":null,"abstract":"<p><p>Conventional wastewater treatment technologies exhibit limited cost-effectiveness for dye removal. This study investigated the decolorization efficacy and adsorption behavior of spent mushroom substrate (SMS) and its aqueous extract (SMS-T) toward ten synthetic dyes, both before and after thermal treatment. SMS achieved total decolorization rates of 72.65-97.84% across the tested dyes. Adsorption by the SMS matrix accounted for only 0.68-36.46% of decolorization for Bromophenol Blue, Malachite Green, Methylene Blue, Rose Bengal, and Remazol Brilliant Blue R, substantially lower than the differential removal (8.25-71.63%) between SMS and SMS-T. This indicates the existence of a non-adsorptive decolorization mechanism mediated by water-insoluble SMS components. Comparative analysis of SMS-T before and after heat treatment revealed that thermally processed SMS-T maintained > 30% decolorization efficiency for Methylene Blue, Brilliant Blue G, and Crystal Violet. Notably, decolorization rates for Rose Bengal and Remazol Brilliant Blue R showed no significant difference post-treatment, demonstrating the presence of heat-stable non-enzymatic decolorization mechanisms in SMS-T. Isothermal adsorption studies indicated that the Langmuir model optimally described SMS adsorption for Congo Red, Aniline Blue, Malachite Green, and Remazol Brilliant Blue R. In contrast, the Freundlich model better fitted the other six dyes. Concurrently, SMS achieved effective decolorization and partial toxicity reduction when treating simulated textile effluent (STE). This work proposes two complementary mechanisms governing SMS-mediated decolorization: non-adsorptive decolorization by water-insoluble SMS fractions and heat-resilient non-enzymatic pathways in SMS-T. These findings establish SMS as a multifunctional bioremediation agent, providing an integrated physicochemical-biological strategy for treating complex dye-laden wastewater.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"351"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A culture-independent 16 S rRNA gene-based profiling of the sugarcane phyllosphere bacterial community in Western Uttar Pradesh, India.","authors":"Aditya Pathak, Pankaj Kumar, Himanshu Kumar, Jitender Singh, Mahesh Kumar Bharti, Rekha Dixit, Kamal Khilari","doi":"10.1007/s11274-025-04541-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04541-1","url":null,"abstract":"<p><p>This study characterizes the sugarcane (Saccharum officinarum L.) phyllosphere bacterial community in Western Uttar Pradesh, India, using culture-independent16S rRNA gene sequencing. Samples from saline-affected Shamli (S-group) and agrochemical-exposed Hapur (H-group) revealed distinct taxonomic profiles dominated by Pseudomonadota (50.3%), Bacteroidota (22.4%),and Bacillota (12.8%),with Pantoea, Sphingomonas, and Priestia as key genera. Hapur exhibited higher alpha diversity (Shannon index: 4.46), while beta diversity showed clear geographical clustering (Bray-Curtis dissimilarity: 0.26-0.63). Contrary to expectations, Shamli's phyllosphere, despite soil salinity, was enriched with xenobiotic-degrading taxa (Acinetobacter, Pseudomonas), likely due to foliar agrochemical deposition via saline irrigation. Hapur's community, though exposed to agrochemicals, showed abundant plant-growth-promoting taxa (Streptomyces, Bacillus), potentially facilitated by organic amendments. Functional prediction highlighted stress-response pathways in Shamli (e.g., sulfoglycolysis, reductive TCA cycle) and growth-promoting pathways in Hapur (e.g., polyamine biosynthesis). The absence of the endophytic symbiont Gluconacetobacter suggests niche exclusion by dominant phyllosphere colonizers.These findings demonstrate how phyllosphere communities diverge from soil microbiomes in stress adaptation, providing insights for region-specific microbiome management in sugarcane cultivation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"328"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-oriented assessment of bovine tuberculosis in Tunisian cattle: IDR, PCR_Mpb70 and serological test prediction based on AI approaches.","authors":"Said Jaajaa, Wafa Bouglita, Oussema Khamessi, Ghada Mahjoub, Sana Dhaouadi, Ahmed Rjeb, Kais Ghedira, Hanen Smadhi, Ramzi Boubaker Elandoulsi","doi":"10.1007/s11274-025-04557-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04557-7","url":null,"abstract":"<p><p>Bovine tuberculosis (bTB), caused by Mycobacterium bovis (M. bovis), remains a major zoonotic and economically burdensome disease worldwide. In Tunisia, where bTB has remained present for many years, Efforts to eliminate the disease have been slowed down by limited diagnostic tools and a lack of resources. The tuberculin Intra Dermal Reaction test (IDR), also known as Tuberculin Skin Test (TST), is still the main tool for surveillance, but its low sensitivity and specificity show the need for better diagnostic methods. This study evaluated the diagnostic performance of IDR, PCR_Mpb70 and three serological rapid tests: Quickvet Ab^® (Ac1, detecting antibodies against a recombinant M.bovis antigen), Vetdiagnostix Ab^® (Ac2, detecting antibodies against a recombinant MPB70/MPB83 fusion protein), and Vetdiagnostix Ag^® (Monoclonal antibodies against Bovine IFN-γ detecting a native M. bovis antigen complex) in Tunisian cattle (n = 32). Based on AI, Bayesian Latent Class Model (BLCM) analysis, PCR_Mpb70 was identified as the most reliable reference standard due to its high sensitivity (Se) and perfect specificity (Sp) when assessed alongside other tests. Using PCR_Mpb70 as a proxy gold standard, supervised machine learning via the Random Forest algorithm was employed to assess the predictive performance of the individual and combined diagnostic tests. As a main result, a high bTB prevalence (46.37%) was confirmed in the tested animals, although prevalence estimates varied considerably depending on the diagnostic test used (average ± 9.76). The combinations IDR2/Ac2, IDR2/Ag, and IDR2/Ac2/Ag pair a second IntraDermal test (IDR2) with antibody tests for Ac2, Ag, or both, proved to be the most informative and complementary alternatives to PCR. These approaches provide practical and effective diagnostic options for field settings where access to molecular testing is limited or unavailable.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"339"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel genome exponential amplification reaction (GEAR) assay for rapid and ultrasensitive detection of Salmonella.","authors":"A A P Milton, K Srinivas, Zakir Hussain, D Bhargavi, A G Momin, G Bhuvana Priya, Heiborkie Shilla, Samir Das, S Ghatak, Girish P Shivanagowda","doi":"10.1007/s11274-025-04538-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04538-w","url":null,"abstract":"<p><p>Salmonella is a major contributor to foodborne disease outbreaks worldwide, making its surveillance crucial from both public well-being and food safety perspectives. Rapid and sensitive methods for monitoring Salmonella contamination in food stuffs are especially essential in low-resource settings to help avert outbreaks and enable timely product recalls. In the present work, a novel genome exponential amplification reaction (GEAR) based detection assay was developed for Salmonella by amplifying the invA gene. The inclusive and exclusive specificity of the test was verified to be 100% employing reference strains of Salmonella and other non-target pathogenic bacteria. The reaction sensitivity of the GEAR was determined to be 32.4 fg/µL or fg/reaction, making it 100 times more sensitive than conventional molecular techniques such as PCR and real-time PCR. The method sensitivity of the developed GEAR test was evaluated using experimentally contaminated pork samples, showing a detection limit of 212 CFU/g for culture independent direct detection and 2.12 CFU/g when combined with 6 h of enrichment. The assay was further validated on 140 real-world pork samples and was evaluated alongside real-time PCR and culture methods. It successfully detected five culture-positive samples identified by the ISO 6579:2002 isolation method, demonstrating 100% accuracy. This ultrasensitive, rapid assay, which does not require sophisticated instrumentation, presents a viable alternative to time-consuming microbiological methods and resource-intensive molecular techniques. To our knowledge, this is the first report of a GEAR assay developed and validated for the detection of Salmonella.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"340"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic responses of Saccharomyces cerevisiae and Hanseniaspora uvarum during separated co-fermentation.","authors":"Yajie Yu, Wanying Zhu, Desire Nzoyisaba, Xiuyan Zhang","doi":"10.1007/s11274-025-04574-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04574-6","url":null,"abstract":"<p><p>Hanseniaspora uvarum is a predominant yeast species in vineyards and some strains demonstrate oenological potential by enhancing the aroma complexity and reducing the content of volatile acid and ethanol in wine. H. uvarum kept in our lab could improve the flavor and quality of wine when co-fermented with Saccharomyces cerevisiae, but its growth was inhibited by S. cerevisiae. To investigate the interaction mechanisms of S. cerevisiae and H. uvarum, double-compartment fermentor with dialysis tube of 1000 kDa MWCO was selected for separated co-fermentation (SCF) in synthetic grape juice at 25℃ without agitation. Differentially expressed genes (DEGs) from S. cerevisiae and H. uvarum from SCF were analyzed using RNA-seq. H. uvarum exhibited a greater number of DEGs at both T1 (201) and T2 (444) compared to S. cerevisiae (T1: 158, T2: 294). Genes related to antioxidant and damage repair systems and glucose transportation and metabolism in S. cerevisiae were up-regulated at T1 and T2. Genes related to flocculation were up-regulated at T2. In contrast, H. uvarum from SCF down-regulated genes related to these pathways and up-regulated genes related to amino acid transportation and sulfate assimilation. These genes and pathway might potentially contribute to the interaction between S. cerevisiae and H. uvarum. Research results will provide a theoretical basis for yeast interaction mechanisms and research on other microbial interactions.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"335"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing triterpenoids production in Ganoderma multipileum submerged cultures using caffeine as a P450 inducer and limonene as a precursor.","authors":"Te-Wei MaYan, Yu-Huan Chang","doi":"10.1007/s11274-025-04556-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04556-8","url":null,"abstract":"<p><p>Ganoderma multipileum, a medicinal mushroom, produces valuable triterpenoids. This study investigates the effect of caffeine, a food additive that induces cytochrome P450 (P450), on the production of crude triterpenoids and biomass of G. multipileum-the experimental procedure involved adding caffeine and Limonene to the basal culture at different concentrations and times. When 0.4% caffeine was added on the 6th day, the triterpenoid content reached 53.72mg/g DW on the 15th day, which was 8.4 times that of the control. In addition, total polyphenol content also increased, reaching 1.96mg/g DW (74% higher than control), suggesting that caffeine exerts a broader metabolic influence beyond triterpenoid synthesis. This suggests that caffeine, acting as a P450 inducer, likely enhances the activity and abundance of triterpenoid synthase in G. multipileum, contributing to the observed increase in triterpenoid production. Continuing the experiment, when 1% (v/v) Limonene was added on the 6th day, the triterpenoid and total polyphenol contents reached 66.60mg/g DW and 3.72mg/g DW, respectively, on the 15th day, which were 10.4 times and 3.3 times that of the control. This finding indicates that when caffeine induces the production of more triterpene synthase, additional precursors are required, and thus, the addition of limonene enhances triterpenoid production. This study demonstrates that significant increases in triterpenoid production can be achieved using a food additive inducer without the need for a drug inducer.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"333"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}