{"title":"Unveiling the novel role of several Flavobacteriaceae isolates: Cultivable bacteria associated with Porolithon onkodes induce Pocillopora damicornis larval settlement and metamorphosis.","authors":"Zhiliang Xiao, Lijuan Long, Fangfang Yang","doi":"10.1007/s11274-026-05327-9","DOIUrl":"10.1007/s11274-026-05327-9","url":null,"abstract":"<p><p>Crustose coralline algae (CCA) are pivotal for coral larval settlement and metamorphosis, yet the functional roles of their associated bacteria in this critical process remain underexplored. Here, we systematically isolated 554 cultivable bacteria from the dominant CCA species Porolithon onkodes and assessed their ability to induce larval settlement and metamorphosis of Pocillopora damicornis. The isolates were dominated by Pseudomonadota (50.72%) and Bacteroidota (32.85%), with 57.40% exhibiting < 98.60% 16S rRNA gene similarity to their closest type-strain matches, suggesting that Por. onkodes may harbor substantial numbers of potentially undescribed or poorly characterized bacteria. Notably, 33 isolates significantly induced larval settlement and metamorphosis, with rates exceeding 40.00% after 96 h, mainly including 22 Flavobacteriaceae and 9 Bacillaceae isolates, substantially expanding the known taxonomic range of bacteria involved in this process. Further investigation on Flavobacteriaceae isolate B1405 (a highly active inducer) revealed that its inductive activity was tightly dependent on growth phase and biofilm age: logarithmic- or stationary-phase cells induced > 38.00% settlement, while 3-day-old biofilms significantly enhanced the settlement rate to > 60.00%. These findings fill critical gaps in CCA-bacteria-coral interactions and highlight specific cultivable isolates within Flavobacteriaceae and Bacillaceae as potential microbial candidates for further investigation in coral settlement and restoration applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149148631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Organic-inorganic composite inoculant promotes carbon stabilization in acidic lateritic red soil by redirecting microbial catabolism toward anabolism.","authors":"Junyu Xiang, Qian Zhang, Zicong Xiong, Zhongning Liu, Juanjuan Yin, Congyao Shi, Binhong Qin, Weiwei Yang, Chaolan Zhang","doi":"10.1007/s11274-026-05318-w","DOIUrl":"10.1007/s11274-026-05318-w","url":null,"abstract":"<p><p>Acidification, nutrient deficiency, and poor carbon pool stability are key bottlenecks restricting the sustainable development of agriculture in lateritic red soils. In this study, we isolated strains GC05 (Paracoccus communis) and GC07 (Advenella kashmirensis). A composite microbial consortium was constructed and then combined with a biochar-montmorillonite-chicken manure carrier to produce an organic-inorganic composite microbial inoculant. After applying the inoculant to acidic lateritic red soil, we investigated its mechanisms in simultaneously ameliorating acidic lateritic red soil and driving soil organic carbon stabilization. After a 30-day incubation, the inoculant ameliorated the soil environment and enhanced both nutrient availability and carbon stability. Soil pH increased from 4.86 to 5.76, the ratio of recalcitrant carbon to total organic carbon increased from 0.72 to 0.86. The inoculant promoted the enrichment of carbon assimilation-dominant functional microbial taxa. Microbial carbon use efficiency increased from 0.02 to 0.41, and the metabolic focus shifted from enzymatic depolymerization and catabolism to biomass synthesis and anabolism. Structural equation modeling further suggested that microbial biomass exerted a dominant direct effect on the stable soil carbon pool, driving the conversion of labile organic carbon into recalcitrant organic carbon via biomass synthesis and microbial necromass accumulation, which constituted an early-stage microbial carbon pump. This study provides a theoretical foundation and practical strategy for the development of bioaugmented technologies with potential for long‑term carbon sequestration in acidic lateritic red soils.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paula Derksen Macruz, Giovanna Picoli Libel, Lucas Haruo Nakayama Pereira, Juliano Cesar da Silva, Sergio Antônio Barraca, Eduardo Radovanovic, Eduardo Jorge Pilau
{"title":"Electrospun PVA nanofibers improve the storage stability of Bacillus amyloliquefaciens fermentation broth.","authors":"Paula Derksen Macruz, Giovanna Picoli Libel, Lucas Haruo Nakayama Pereira, Juliano Cesar da Silva, Sergio Antônio Barraca, Eduardo Radovanovic, Eduardo Jorge Pilau","doi":"10.1007/s11274-026-05334-w","DOIUrl":"10.1007/s11274-026-05334-w","url":null,"abstract":"<p><p>The development of robust, stable microbial formulations remains a major challenge for agricultural use. We report an electrospinning-based formulation that encapsulates Bacillus amyloliquefaciens fermentation broth within poly(vinyl alcohol) (PVA) nanofibers to enhance storage stability and preserve microbial viability. Electrospun nanofiber mats were obtained from PVA formulations containing the fermentation broth, and their morphology, chemical profile, mechanical properties, and microbial viability were evaluated. Morphological, spectroscopic, mechanical, and microbiological analyses demonstrated successful incorporation of the fermentation broth into the PVA matrix and retention of viable Bacillus cells after electrospinning, with comparative estimates of viable cell recovery of 3.3 × 10<sup>7</sup> CFU cm<sup>- 2</sup> in the electrospun fibers and 8.3 × 10<sup>7</sup> CFU mL<sup>- 1</sup> in the polymeric spinning solution before electrospinning. Nanofibers stored under ambient conditions for up to one year still yielded viable cells. Collectively, these findings support the potential of electrospun PVA nanofibers as a formulation strategy for microbial stabilization and warrant further investigation toward the development of microbial formulations with improved storage characteristics.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuhang Pang, Yixin Song, Ying Wang, Jilian Wang, Mingyuan Li
{"title":"Synthetic microbial communities mediate maize growth and soil microecology under saline-alkali stress.","authors":"Yuhang Pang, Yixin Song, Ying Wang, Jilian Wang, Mingyuan Li","doi":"10.1007/s11274-026-05330-0","DOIUrl":"10.1007/s11274-026-05330-0","url":null,"abstract":"<p><p>Soil salinization results in soil degradation and a decline in productivity. Synthetic microbial communities (SynComs) perform more stably than single strains, providing a new approach for saline-alkali soil improvement. Two synthetic communities SynCom1 and SynCom2 were assembled from plant rhizosphere salt-tolerant isolates via antagonism and co-culture assays. Six treatments were established to test SynCom inoculation effects. A represented non-sterilized soil added with SynCom1; B represented non-sterilized soil added with SynCom2, C represented non-sterilized soil supplemented with LB medium as control; D represented sterilized soil added with SynCom1; E represented sterilized soil added with SynCom2; F represents sterilized soil supplemented with LB medium as control. This design was used to distinguish the respective effects of SynComs, indigenous soil microorganisms and fermentation medium. SynComs enhanced the growth of maize plants, and decrease soil pH and electrical conductivity. Specifically, SynCom1 increased the activities of soil urease, alkaline phosphatase, and dehydrogenase under unsterilized environment (P < 0.05). The absolute quantification of 16 S RNA revealed that both agents caused an increase of absolute bacterial abundance in sterilized soil, compared to unsterilized soil. Notably, SynCom2 led to an increase in the beneficial genera including Streptomyces and Micromonospora. In unsterilized soil, the co-occurrence network of SynCom1 exhibited the highest complexity. However, in sterilized soil, the network inoculated with SynCom2 showed greater stability than that with SynCom1. Functional prediction revealed that SynCom1 mainly participated in basal energy metabolism, while SynCom2 activated secondary metabolism in non-sterilized soils. In sterilized soils, the two communities were enriched in colonization adaptation and nutrient metabolism functions, respectively. Our study provides a practical basis for the improvement of saline-alkali soils using SynComs.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149148823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction: From metabolites to membrane vesicles: Unifying gut microbial signals in obesity, t2dm, and MASLD.","authors":"Barathan Muttiah, Gopinath Venkatraman, Puteri Shafinaz Abdul-Rahman","doi":"10.1007/s11274-026-05263-8","DOIUrl":"https://doi.org/10.1007/s11274-026-05263-8","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health.","authors":"Mateus Rodrigues Alves, Nicolly Soares Ferreira, Max Sulivan Fontes, Mariana Fabri Lima, Marcelo Henrique Otenio, Mariana Drummond Costa Ignacchiti, Juliana Alves Resende","doi":"10.1007/s11274-026-05319-9","DOIUrl":"10.1007/s11274-026-05319-9","url":null,"abstract":"<p><p>Antimicrobial-resistant bacteria in agricultural waste represent a critical One Health concern. Anaerobic digestion of livestock waste is widely promoted; however, the fate of antimicrobial resistance and biocide tolerance in full-scale systems remains unclear, constraining safe digestate reuse. This study assessed phenotypic and genotypic resistance in Escherichia coli-like enterobacteria isolated from effluent and biofertilizer in a full-scale dairy anaerobic digestion system. A total of 173 isolates were screened for antimicrobial resistance genes (ARGs), antimicrobial susceptibility, multidrug resistance (MDR), and biocide inhibition-zone responses. No diarrheagenic virulence genes were detected. At least one screened ARG was detected in 91.0% of effluent and 83.3% of biofertilizer isolates. qacE∆1 was the most frequent marker and occurred significantly more often in effluent (70.8%) than biofertilizer isolates (44.0%; q = 0.00291). Phenotypic resistance to at least one antimicrobial was observed in 27.0% and 34.5% of effluent and biofertilizer isolates, respectively, while MDR occurred in 2.2% and 9.5% (p = 0.0521). tet(a) carriage was strongly associated with tetracycline resistance (OR = 20.9; q < 0.001). H<sub>2</sub>O<sub>2</sub> inhibition zones were significantly smaller among biofertilizer isolates, whereas Lactic Acid (LA) yielded 6-mm measurements in all isolates and in the reference strain. These findings demonstrate that anaerobic digestion does not fully eliminate culturable resistant enterobacteria and underscore the need for integrated resistance monitoring in dairy-waste management.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13649801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring the use of Leucobacter komagatae as an eco-friendly bio-inoculant to enhance sugarcane (Saccharum officinarum) growth and yield under drought conditions.","authors":"Abhay Kumar Srivastava, Sapna Chandwani, Natarajan Amaresan, Sudhir Kumar Shukla, Raghvendra Tiwari, Priyanka Srivastava, Shyam Sundar Rao, Amit Srivastava, Vijai Prakash Jaiswal, Rajesh Kumar Tiwari","doi":"10.1007/s11274-026-05331-z","DOIUrl":"10.1007/s11274-026-05331-z","url":null,"abstract":"<p><p>Drought occurrence owing to climate change is a major constraint to attain sustainable agriculture, severely limiting crop growth and productivity. Sugarcane (Saccharum spp. hybrids), a key crop in tropical and subtropical regions, is particularly susceptible to drought stress because of its extended growth duration and high-water requirements. In this study, previously identified four plant growth promoting Leucobacter komagatae (ASBD2, ASBD15, ASBD19 and ASBD30) were evaluated under greenhouse conditions (pot culture study) over two consecutive years (2023-24 and 2024-25). Of these four isolates, L. komagatae ASBD19 showed the strongest performance for several physiological and drought-response attributes, whereas ASBD2 and ASBD15 were more effective for biomass accumulation and nutrient acquisition, respectively. The ASBD 19 strain exhibited significantly enhanced germination rate (70% and 66% at 60 days after planting (DAP)), tillering ( 8 and 9 tillers/pot at 180 days), plant height (118 cm and 127 cm at 270 days), photosynthetic activity at grand growth stage (PAR incident on leaf surface 1150.7 µmol m-<sup>2</sup> s-<sup>1</sup> ; transpiration rate 3.85 mol m-<sup>2</sup> s-<sup>1</sup>; photosynthetic rate 18.15 µmol m-<sup>2</sup> s-<sup>1</sup>; and stomatal conductance 0.93 mol m-<sup>2</sup> s-<sup>1</sup>), and increased cane length (165 cm and 168 cm) and cane weight (0.75 and 0.54 kg) at harvesting stage of the sugarcane compared to the control under drought stress. In conclusion, these findings suggest that the native L. komagatae strains have potential as sustainable bioinoculants for drought-prone sugarcane, with ASBD19 for maintaining physiological performance and drought adaptation, whereas ASBD2 and ASBD15 for improving nutrient acquisition and biomass production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Praisy Joy Bell I, Sadhana Sundararajan, Rajiniraja Muniyan
{"title":"Integrated in-silico and in-vitro evaluation of silybin as a potential quorum-sensing modulator against Acinetobacter baumannii and Staphylococcus aureus.","authors":"Praisy Joy Bell I, Sadhana Sundararajan, Rajiniraja Muniyan","doi":"10.1007/s11274-026-05325-x","DOIUrl":"10.1007/s11274-026-05325-x","url":null,"abstract":"<p><p>The emergence of multi-drug resistance in the class of ESKAPE pathogens such as Acinetobacter baumannii and Staphylococcus aureus has led to the search for anti-virulence mechanisms to overcome resistance posed by the existing antibiotics. Quorum sensing (QS), mediated by AbaI/AbaR circuits in A. baumannii and AgrA/AgrC circuits in S. aureus, regulates biofilm formation and virulence factor production in the two species, thereby serving as potential targets for phytocompound action. In the current study silybin, a flavonoid lignan from Silybum marianum, emerged as a potential QS modulator against both pathogens based on an integrated computational and experimental approach. Molecular docking studies predicted favourable interactions of silybin to the four regulatory proteins (-14.76 to -12.34 kcal/mol for AbaI and AbaR, -10.20 to -9.83 kcal/mol for AgrA and AgrC), binding functionally important amino acid residues in their active sites. Molecular dynamics simulations lasting 500 ns revealed that silybin binding decreased backbone and residue-level flexibility, increased compactness, reduced solvent-accessible surface area and restricted collective motion in comparison with their respective apo-proteins in all four targets. MM-GBSA calculations together with per-residue decomposition and contact analysis emphasized the role of van der Waals and electrostatic interactions contributed to the predicted binding energetics of the protein-ligand complexes. Silybin displayed in-vitro antibacterial activity, time-dependent inhibitory activity, and concentration-dependent antibiofilm activity up to 60% in A. baumannii and up to 70% in S. aureus. Downregulation was observed for genes involved in virulence in S. aureus, but not for abaI/abaR in A. baumannii. Overall, the findings support silybin as a potential QS- associated modulator with anti-biofilm activity against both pathogens. However, additional studies are required to establish QS-specific activity, selectivity, cytotoxicity, pharmacokinetic properties, and in-vivo efficacy.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sustainable polyhydroxybutyrate production and characterization from Chlorella vulgaris cultivated on waste-derived carbon sources.","authors":"Oyku Alp, Raziye Ozturk Urek","doi":"10.1007/s11274-026-05316-y","DOIUrl":"10.1007/s11274-026-05316-y","url":null,"abstract":"<p><p>Polyhydroxybutyrate (PHB) is a biopolymer produced by various microorganisms for carbon and energy storage and stands out in terms of sustainable bioplastic production. PHB is a biodegradable and sustainable substitute for petrochemical-based plastics frequently used nowadays. Interest in waste-based/byproduct carbon sources has recently increased due to the high production cost of microbial PHB, and byproducts and wastes are being evaluated within the scope of zero-waste projects. In this study, the metabolic changes of Chlorella vulgaris were examined in the existence of different substrates including wine vinegar (WV), vinegar wastewater (VW), crude glycerol (CG), and tomato paste wastewater (TPW), and the effects of these conditions on PHB production were evaluated. Changes in cell dry weight, optical density, pH, total reducing sugars, total -nitrogen, -carbohydrates, -proteins, -lipids, and PHB levels were monitored over time during cultivation. 1% (v/v) VW was found to be optimal carbon source/concentration. To optimize extraction, solvent-based and ultrasonic extraction methods were tested for different durations, and the highest yield of 23.280 ± 2.10% was determined after 15 days. The extracted PHB was characterized by FTIR, GC, NMR, TGA, DSC, and SEM analyses. Thus, the characteristic functional groups of PHB obtained from C. vulgaris have been confirmed. This study provides important data on directing carbon flow to PHB production metabolism in mixotrophic microalgal cultures using waste/byproduct-based carbon sources and demonstrates that the potential of C. vulgaris in bioplastic production can be revealed through metabolic and nutritional optimizations.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haris Maqbool, Nadeem Ullah, Iqra Fazil, Mohsin Khan, Muhammad Aaqil Khan, Hassan Javed Chaudhary, Junqiang Zheng, Iffat Naz
{"title":"Bacterial extracellular vesicles as emerging biostimulants in plant microbe signaling and stress tolerance.","authors":"Haris Maqbool, Nadeem Ullah, Iqra Fazil, Mohsin Khan, Muhammad Aaqil Khan, Hassan Javed Chaudhary, Junqiang Zheng, Iffat Naz","doi":"10.1007/s11274-026-05301-5","DOIUrl":"10.1007/s11274-026-05301-5","url":null,"abstract":"<p><p>Bacterial extracellular vesicles (BEVs) are nanosized membrane-bound nanoparticles naturally secreted by Gram-negative and Gram-positive bacteria. These vesicles mediate intercellular communication through delivery of diverse bioactive cargo, including proteins, lipids, nucleic acids, metabolites, and signaling molecules. Initially recognized as virulence determinants in pathogenic bacteria, BEVs are increasingly recognized as multifunctional platforms with potential applications in sustainable agriculture. This review examines BEV biogenesis, cargo composition, plant uptake, and emerging roles in plant growth promotion, stress adaptation, and disease resistance. Emphasis is placed on BEV-mediated modulation of plant immunity through pattern recognition receptors (PRRs), pattern-triggered immunity (PTI), and induced systemic resistance (ISR). Potential roles of vesicle-associated phytohormones, ACC deaminase, siderophores, extracellular enzymes, and regulatory RNAs in nutrient acquisition, phytohormone homeostasis, and stress responses are critically evaluated. Emerging evidence linking BEVs to drought, salinity, heat, heavy metal, and nutrient-deficiency responses is examined, with emphasis on antioxidant defense, osmotic adjustment, ion homeostasis, and stress-responsive signaling. The review also discusses engineered BEVs as potential biostimulants, nano-biofertilizers, biocontrol agents, and precision delivery systems, including CRISPR-based engineering, synthetic vesicles, AI-guided cargo optimization, and smart nanoformulations. Major challenges include scalable production, standardization, biosafety, regulation, and field validation. However, critical knowledge gaps remain regarding BEV perception, cellular uptake, cargo delivery, and the causal basis of plant responses, together with uncertainties surrounding biosafety, reproducibility, and performance under field conditions. Collectively, BEVs represent promising biological nanocarriers for climate-resilient and sustainable agriculture, but further mechanistic, methodological, and field-based research is required to establish their reliability and practical agricultural potential.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}