World journal of microbiology & biotechnology最新文献

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Control of diarrhea and intestinal damage caused by enteropathogenic Escherichia coli using infant formula fermented with Lacticaseibacillus paracasei BMK2005: in vitro and in vivo evidences. 用副干酪乳杆菌BMK2005发酵婴儿配方奶粉控制肠致病性大肠杆菌引起的腹泻和肠道损伤:体外和体内证据
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04295-w
Kamel Bendjeddou, Amel Ait Meddour, Ahmed Adjebli, Roza Ourtirane, Djamila Sadoun, Djamel Drider
{"title":"Control of diarrhea and intestinal damage caused by enteropathogenic Escherichia coli using infant formula fermented with Lacticaseibacillus paracasei BMK2005: in vitro and in vivo evidences.","authors":"Kamel Bendjeddou, Amel Ait Meddour, Ahmed Adjebli, Roza Ourtirane, Djamila Sadoun, Djamel Drider","doi":"10.1007/s11274-025-04295-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04295-w","url":null,"abstract":"<p><p>Lacticaseibacillus paracasei BMK2005 (L. paracasei BMK2005) was isolated from the feces of a 1-year-old healthy Algerian infant. This strain, identified by 16S rDNA sequencing and API system, has demonstrated a remarkable antibacterial activity against enteropathogenic Escherichia coli in vitro and in vivo. In addition, this strain was shown to survive in a variety of environmental conditions that mimic the gastrointestinal tract conditions such as different pH values, bile salts and digestive enzymes. Moreover, L. paracasei BMK2005 was γ-hemolytic and sensitive to various antibiotics, supporting a safe characteristic for its application. Next, the anti-EPEC activity was then evaluated in an infant formula composed of 50% whey and 50% skim milk. A significant reduction in EPEC counts was observed after 24 h of incubation. These inhibitory properties were confirmed in an animal model using ten holoxenic rabbits made diarrheic by EPEC ingestion. L. paracasei BMK2005 was able to reduce the number of EPEC in the feces of diarrheic animals treated with the milk formula fermented by this strain. To support this study, histopathologic sections of the intestines of treated and untreated rabbits revealed clear injury in the case of animals infected with EPEC, an effect characterized by damaged villosities, while normal villosities were observed in samples from control or treated animals, delineating the protective effect of L. paracasei BMK2005.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"209"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unveiling the probiotic potential of Enterococcus spp.: Mechanisms and roles in animal and human health, A comprehensive review. 揭示肠球菌的益生菌潜力:机制及其在动物和人类健康中的作用,综述。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04389-5
Donia Mechoub, Nacima Meguenni, Yacine Titouche, Ramzi Boubaker Elandoulsi, Sana Dhaouadi
{"title":"Unveiling the probiotic potential of Enterococcus spp.: Mechanisms and roles in animal and human health, A comprehensive review.","authors":"Donia Mechoub, Nacima Meguenni, Yacine Titouche, Ramzi Boubaker Elandoulsi, Sana Dhaouadi","doi":"10.1007/s11274-025-04389-5","DOIUrl":"https://doi.org/10.1007/s11274-025-04389-5","url":null,"abstract":"<p><p>Enterococci, a subset of lactic acid bacteria, are ubiquitous in a variety of ecosystems, including the gastrointestinal tracts of mammalian and avian species. Due to their extreme adaptability, enterococci thrive in exceptionally unfavorable conditions characterized by high salinity levels, exposure to bile salts, a wide range of pH variations and high-temperature thresholds. Investigation into enterococcal strains as potential probiotics has encompassed the fields of human health, animal husbandry and companion animal welfare. In the context of human applications, the objectives are related to increasing immune responses, reducing cholesterol levels and providing anti-inflammatory and antioxidant attributes. Similarly, the livestock sector has recognized the potential of enterococci to stimulate growth, inhibit pathogen proliferation and amplify immune reactivity. Pet health interventions using enterococci aim to balance the microbiota, boost immune function and improve the animal's general well-being. The mechanisms of probiotic effects mediated by enterococci are manifold, including the production of enterocins, improvement of cholesterol levels, immunomodulation, antioxidant activities, attenuation of inflammatory cascades and the support of liver functions. While enterococcal probiotics hold great promises for optimizing human and animal health, safety considerations must be taken into account. Given the pathogenic character of enterococci and their capacity to carry antibiotic resistance determinants, rigorous evaluation of safety profiles is an imperative prelude to their judicious use as probiotics.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"214"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nucleic acid conjugated gold nanoparticles for biosensing applications: a review. 核酸共轭金纳米颗粒在生物传感中的应用综述。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04454-z
Tejaswini P Patil, Anuja A Vibhute, Ajayan Vinu, Arpita Pandey-Tiwari
{"title":"Nucleic acid conjugated gold nanoparticles for biosensing applications: a review.","authors":"Tejaswini P Patil, Anuja A Vibhute, Ajayan Vinu, Arpita Pandey-Tiwari","doi":"10.1007/s11274-025-04454-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04454-z","url":null,"abstract":"<p><p>Gold nanoparticles (AuNPs) have gained widespread use in the development of biosensors for molecular diagnostics due to their ease of functionalization and distinctive optical properties, which are attributed to plasmonic effects. Functionalization strategies are widely applied to manipulate the properties of AuNPs which are then applied for various applications including drug delivery, biosensing, and bioimaging. Among the functionalised AuNPs, nucleic acid-conjugated gold nanoparticles (AuNPs-NA conjugate) have emerged as a powerful platform for biosensing applications due to their unique optical properties, biocompatibility, and ease of functionalization. This review provides an in-depth examination of the integration of nucleic acids with AuNPs for a wide range of biosensing technologies, including detection of pathogens and biomarkers. This review also discusses various methods of conjugating nucleic acids to gold nanoparticles, including covalent bonding, electrostatic interactions, and thiol-based linkages, while highlighting the key factors that influence the stability, specificity, and sensitivity of these conjugates. The paper further explores the optical, electrochemical, and fluorescent biosensing mechanisms enabled by AuNPs-NA conjugates. Finally, it provides a perspective on the future directions of AuNPs-NA conjugates in the development of rapid, cost-effective, and highly sensitive diagnostic tools for clinical and environmental monitoring.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"219"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biosurfactant type and introduction order impact the morphology of biological CaCO3 during the MICP process. 生物表面活性剂的种类和引入顺序影响生物CaCO3在MICP过程中的形态。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04445-0
Weida Wang, Mingtao Zhu, Changxiong Zou, Tingting Zhang, Jianhong Han, Zhaoyu Wang
{"title":"Biosurfactant type and introduction order impact the morphology of biological CaCO<sub>3</sub> during the MICP process.","authors":"Weida Wang, Mingtao Zhu, Changxiong Zou, Tingting Zhang, Jianhong Han, Zhaoyu Wang","doi":"10.1007/s11274-025-04445-0","DOIUrl":"https://doi.org/10.1007/s11274-025-04445-0","url":null,"abstract":"<p><p>In recent years, microbially induced carbonate precipitation (MICP) technology has attracted considerable attention; however, the morphology of biological calcium carbonate (Bio-CaCO<sub>3</sub>) produced in MICP exhibites variability, which impacts its application. This study employed rhamnolipids and sophorolipids to investigate the impact of biosurfactant type and introduction order on the morphology of Bio-CaCO<sub>3</sub> in the MICP process. The findings indicated that the biosurfactant type and introduction order influenced the morphology of Bio-CaCO<sub>3</sub> and the composition of dissolved organic matter (DOM) in the solution. In the absence of biosurfactants, the Bio-CaCO<sub>3</sub> was primarily composed of rhombic calcite and spherical vaterite. The pre-introduction of rhamnolipids was observed to promote the formation of fasciculated agglomerates of calcite, while the pre-introduction of sophorolipids was observed to promote the formation of spherical agglomerates of calcite. The post-introduction rhamnolipids or sophorolipids were observed to stabilize hollow, spherical vaterite. The findings of the study indicated that the biosurfactant type and introduction order influenced the types and amount of DOM in solution, which in turn affected the MICP process and the morphology of Bio-CaCO<sub>3</sub>. This study provides a theoretical foundation for the regulation of the morphology of Bio-CaCO<sub>3</sub> and the application of MICP technology.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"205"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deciphering soil nematode-bacteria-fungi community composition and functional dynamics in coffee agroecosystems under conventional and sustainable management practices in Costa Rica. 哥斯达黎加咖啡农业生态系统中传统和可持续管理措施下土壤线虫-细菌-真菌群落组成和功能动态分析
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04407-6
José Andrés Rojas-Chacón, Fabián Echeverría-Beirute, José Pablo Jiménez-Madrigal, Ingrid Varela-Benavides, Valeria Faggioli, Dirk Berkelmann, Andrés Gatica-Arias
{"title":"Deciphering soil nematode-bacteria-fungi community composition and functional dynamics in coffee agroecosystems under conventional and sustainable management practices in Costa Rica.","authors":"José Andrés Rojas-Chacón, Fabián Echeverría-Beirute, José Pablo Jiménez-Madrigal, Ingrid Varela-Benavides, Valeria Faggioli, Dirk Berkelmann, Andrés Gatica-Arias","doi":"10.1007/s11274-025-04407-6","DOIUrl":"https://doi.org/10.1007/s11274-025-04407-6","url":null,"abstract":"<p><p>Understanding the interactions between soil bacteria, fungi, and nematodes in coffee agroecosystems is crucial for optimizing sustainable agriculture. This study investigated the composition and functional dynamics of these communities under conventional and sustainable management systems. Soil samples were collected from three major coffee-growing regions in Costa Rica, representing different agricultural regimes. Nematode community was analyzed using optical microscopy, while microbial communities were analyzed using high-throughput sequencing. In both cases, bioinformatic tools were used for functional prediction based on taxonomy.. Herbivorous nematodes dominated both systems, while bacterivores (Rhabditidae, Cephalobidae) and fungivores (Aphelenchoidae) were significantly more abundant in soils subject to sustainable practice (p < 0.05). Nematode maturity indices and food web diagnostics showed no significant differences between systems, even though metabolic footprints related to organic matter decomposition varied (p < 0.05). Bacterial communities were dominated by the phyla Proteobacteria, Acidobacteria, and Chloroflexi, while the fungal community was largely composed of Ascomycota (53.21% in both systems). The fungal genus Mortierella was particularly prevalent. Soil pH, along with Ca, Mg, K, and extractable acidity, influenced community composition. Functional profiles revealed higher gene abundances linked to nutrient and energy cycling in sustainable systems, particularly phosphorus and sulfur metabolism. Saprotroph-symbiotroph fungi were more common in sustainable soils, while pathotrophic fungi dominated conventional systems. This is the first comprehensive analysis of bacteria, fungi, and nematodes across different agricultural practices in coffee agroecosystems in Costa Rica.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"220"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Superficial incisional surgical site infections experimentally induced by Staphylococcus aureus in mice: the effect of Bdellovibrio bacteriovorus containing dressing. 金黄色葡萄球菌实验性诱导小鼠浅表切口手术部位感染:含乳杆菌弧菌敷料的效果。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04453-0
Gülseren Maraş Baydoğan, Özlem Ceyhan, Pınar Sağıroğlu, Mustafa Ermiş
{"title":"Superficial incisional surgical site infections experimentally induced by Staphylococcus aureus in mice: the effect of Bdellovibrio bacteriovorus containing dressing.","authors":"Gülseren Maraş Baydoğan, Özlem Ceyhan, Pınar Sağıroğlu, Mustafa Ermiş","doi":"10.1007/s11274-025-04453-0","DOIUrl":"10.1007/s11274-025-04453-0","url":null,"abstract":"<p><p>Bdellovibrio bacteriovorus is an agent that stands out with its predatory properties and has recently been used against pathogens that are frequently resistant to antibiotics. The study was conducted experimentally to determine the effect of dressing application containing Bdellovibrio bacteriovorus on superficial incisional surgical site infection caused by Staphylococcus aureus in mice. In the study, mice were divided into 6 different groups, BB: B. bacteriovorus; NC: Negative Control; PC: Positive Control Methicillin Resistant S. aureus; MRSA + BB: Methicillin Resistant S. aureus + B. bacteriovorus dressing; MRSA + V: Methicillin Resistant S. aureus + Vancomycin; MRSA + BB + V: Methicillin Resistant S. aureus + B. bacteriovorus dressing + Vancomycin group. The treatment procedures were applied over a period of 3 days. Infection symptoms were monitored and recorded at the 24th, 48th, and 72nd hours. In the Staphylococcus aureus + Vancomycin group, all mice developed edema, redness, and fever at 24 h. At 48 h, all mice exhibited edema and redness, with 50% showing fever. At 72 h, 70% of the mice showed edema and redness, and 10% showed fever. In the Staphylococcus aureus + Bdellovibrio bacteriovorus + Vancomycin combined treatment group, all mice exhibited edema, redness, and fever at 24 h. At 48 h, only 20% of the mice showed redness. At 72 h, no edema, redness, fever, purulent discharge, or suture dehiscence was observed. Sepsis developed in 2 of 10 mice in the Staphylococcus aureus + Bdellovibrio bacteriovorus + Vancomycin group. The most effective treatment was in the Staphylococcus aureus + Bdellovibrio bacteriovorus + Vancomycin group. It was determined that sepsis findings were the least in the Staphylococcus aureus + Bdellovibrio bacteriovorus + Vancomycin group. B. bacteriovorus holds the potential to be an effective control agent in preventing or slowing resistance development.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"231"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteomic analysis reveals phage-driven metabolic shifts and biofilm disruption in methicillin-resistant Staphylococcus aureus (MRSA). 蛋白质组学分析揭示了耐甲氧西林金黄色葡萄球菌(MRSA)中噬菌体驱动的代谢变化和生物膜破坏。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04397-5
Khulood Hamid Dakheel, Raha Abdul Rahim, Jameel R Al-Obaidi, Nurhanani Razali, Vasantha Kumari Neela, Tan Geok Hun, Khatijah Yusoff
{"title":"Proteomic analysis reveals phage-driven metabolic shifts and biofilm disruption in methicillin-resistant Staphylococcus aureus (MRSA).","authors":"Khulood Hamid Dakheel, Raha Abdul Rahim, Jameel R Al-Obaidi, Nurhanani Razali, Vasantha Kumari Neela, Tan Geok Hun, Khatijah Yusoff","doi":"10.1007/s11274-025-04397-5","DOIUrl":"10.1007/s11274-025-04397-5","url":null,"abstract":"<p><p>Methicillin-resistant Staphylococcus aureus (MRSA) biofilms pose a severe risk to public health, showing resistance to standard antibiotics, which drives the need for novel antibacterial strategies. Bacteriophages have emerged as potential agents against biofilms, especially through their phage-encoded enzymes that disrupt the biofilm matrix, enhancing bacterial susceptibility. In this study, two bacteriophages, UPMK_1 and UPMK_2, were propagated on MRSA strains t127/4 and t223/20, respectively. Biofilms formed by these strains were treated with phages at specified concentrations, followed by protein extraction and analysis. Comparative proteomic profiling was performed using one-dimensional and two-dimensional SDS-PAGE, with protein identification facilitated by MALDI-TOF/TOF MS spectrometry, to observe biofilm degradation effects. Proteomic analysis revealed that phage treatment induced significant changes in biofilm protein expression, particularly with upregulated ribosome-recycling factors and elongation factors linked to enhanced protein synthesis, reflecting a reactivation of amino acid metabolism in the treated biofilms. This was marked by upregulated intracellular proteases like CIpL, which play a role in protein refolding and degradation, critical for phage progeny production and biofilm disruption. Phage treatment demonstrated notable effects on the metabolic and protein synthesis pathways within MRSA biofilms, suggesting that phages can redirect bacterial cellular processes to favour biofilm breakdown. This indicates the potential of bacteriophages as a viable adjunct to traditional antimicrobial approaches, particularly in combating antibiotic-resistant infections like MRSA. The study underscores the efficacy of bacteriophages as anti-biofilm agents, offering a promising strategy to weaken biofilms and combat antibiotic resistance through targeted disruption of bacterial metabolic pathways and biofilm integrity.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"230"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Algae-bacteria symbiotic biofilm system for low carbon nitrogen removal from municipal wastewater: A review. 藻类-细菌共生生物膜系统去除城市污水中低碳氮的研究进展。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04405-8
Zhiyuan Liang, Ying Zhao, Hongbing Ji, Zifu Li
{"title":"Algae-bacteria symbiotic biofilm system for low carbon nitrogen removal from municipal wastewater: A review.","authors":"Zhiyuan Liang, Ying Zhao, Hongbing Ji, Zifu Li","doi":"10.1007/s11274-025-04405-8","DOIUrl":"https://doi.org/10.1007/s11274-025-04405-8","url":null,"abstract":"<p><p>The treatment of municipal wastewater has become a significant challenge due to its intricate composition and low carbon-to-nitrogen ratio. In order to meet the discharge standards, a large amount of energy is consumed. In this context, the incorporation of microalgae into the conventional activated sludge process has become a promising strategy for low-carbon denitrification. This study aims to integrate research on algal-bacterial symbiotic systems with biofilm technology to enhance energy-efficient nitrogen removal in municipal wastewater treatment. Through comprehensive analysis, this paper elucidates (1) the developmental dynamics of algal-bacterial symbioses, (2) the process of combining algal-bacterial symbiotic systems with biofilm systems, (3) the fundamentals and operational determinants of algal-bacterial symbiotic membrane systems, and (4) the potential applications in sustainable water treatment. The proposed hybrid system demonstrates significant potential for carbon-neutral wastewater treatment through synergistic pollutant degradation, offering an innovative approach to address critical challenges in environmental sustainability and water resource management.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"218"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pseudomonas gessardii suppresses Xanthomonas vesicatoria through 4-Dodecylbenzene sulfonic acid interference with virulence proteins. 格氏假单胞菌通过4-十二烷基苯磺酸干扰毒力蛋白抑制发疱性黄单胞菌。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04451-2
Riddha Dey, Seema Devi, Jay Kishor Prasad, Jatin Jangra, Rajnish Kumar, Richa Raghuwanshi
{"title":"Pseudomonas gessardii suppresses Xanthomonas vesicatoria through 4-Dodecylbenzene sulfonic acid interference with virulence proteins.","authors":"Riddha Dey, Seema Devi, Jay Kishor Prasad, Jatin Jangra, Rajnish Kumar, Richa Raghuwanshi","doi":"10.1007/s11274-025-04451-2","DOIUrl":"https://doi.org/10.1007/s11274-025-04451-2","url":null,"abstract":"<p><p>Endophytic bacteria reside asymptomatically inside host plant tissues and have the potential to protect the host plant from phytopathogens and enhance plant immunity. Exploration of the biocontrol mechanism of these beneficial bacteria can assure the development of eco-friendly pesticides for the effective management of phytopathogens. In our study, the isolated endophytic bacteria examined for its antimicrobial potential was identified as Pseudomonas gessardii strain RRBHU-1 based on 16 S rRNA gene sequence analysis. The crude extract containing the secondary metabolites from the P. gessardii strain RRBHU-1 demonstrated antagonistic activity against Xanthomonas vesicatoria, the causal organism of bacterial leaf spot disease in tomato plants. X. vesicatoria when treated with the RRBHU-1 extract showed disruption of the cell membrane, significant ROS production, and substantial antibiofilm activity. The extract separated through TLC and RP-HPLC and characterized using FT-IR, NMR, and HRAMS, and was identified as 4-Dodecylbenzene sulfonic acid (4-DBSA). This study is the first report on the presence of 4-DBSA in the Pseudomonas genera. In silico studies revealed stable interactions and high binding energies of 4-DBSA and the proteins directly involved in Xanthomonas virulence. This study suggests the possible utilization of P. gessardii strain RRBHU-1 as biopesticides against X. vesicatoria, encompassing sustainable agricultural practices.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"204"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering synthetic microbial communities to restructure the phytobiome for plant health and productivity. 工程合成微生物群落重组植物群系,促进植物健康和生产力。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-06-25 DOI: 10.1007/s11274-025-04460-1
Anwesha Sharma, Popy Bora
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