World journal of microbiology & biotechnology最新文献

{"title":"Novel cell factory for the production of 24-epi-ergosterol, an un-natural semi-synthetic precursor for the production of brassinolide in Yarrowia lipolytica.","authors":"Yuanying Wang, Shuxian Liu, Zeyu Sheng, Yun Feng, Yinmiao Wang, Yiqi Jiang, Li Zhu, Mianbin Wu, Lirong Yang, Jianping Lin","doi":"10.1007/s11274-025-04314-w","DOIUrl":"10.1007/s11274-025-04314-w","url":null,"abstract":"<p><p>Brassinolide (BL) is the most bioactive plant growth regulator among Brassinosteroids (BRs), belonging to the sixth class of plant hormones. However, its low natural abundance limits large-scale agricultural applications. An unnatural sterol, 24-epi-ergosterol, was proposed as a semi-synthetic precursor for economic production of BL. Here, we constructed a synthetic pathway for 24-epi-ergosterol in Yarrowia lipolytica, which has abundant acetyl-CoA content and a hydrophobic intracellular environment. Initially, we introduced a mutant plant-derived Δ²⁴⁽²⁸⁾ sterol reductase (Dwf1) into Y. lipolytica to enable 24-epi-ergosterol production. The production of 24-epi-ergosterol was subsequently enhanced by regulating sterol homeostasis, optimizing transcriptional regulators, and overexpressing key pathway genes. Next, the accumulation of 24-epi-ergosterol was further improved by increasing acetyl-CoA levels and adjusting lipid metabolism. Finally, the 24-epi-ergosterol production reached 1626.85 mg/L after optimizing the fermentation conditions and performing a fed-batch culture in a 2 L fermenter. This study represents the first successful de novo synthesis of 24-epi-ergosterol in Y. lipolytica, offering a novel approach for the industrial production of BL.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"98"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broad-spectrum antimicrobial properties of linalool: supporting its pharmacological use in chronic wound infections by pathogens within the ESKAPE group and polymicrobial biofilms.","authors":"Akshatha Rai, Yuvarajan Subramaniyan, Fida Fathima, Punchappady Devasya Rekha","doi":"10.1007/s11274-025-04317-7","DOIUrl":"10.1007/s11274-025-04317-7","url":null,"abstract":"<p><p>Chronic wound infections are caused by biofilm forming opportunistic pathogenic bacteria. The persistence of infection, co-infecting pathogens and prolonged use of antibiotics promote antibiotic resistance hampering healing process due to increased inflammation. Hence, we tested the broad range antibacterial activity of linalool, a bioactive monoterpene commonly present in many essential oils having anti-inflammatory and antimicrobial activities to target different opportunistic pathogens commonly found in the chronic wound. We included some of the common pathogens such as Acinetobacter baumannii, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus, to study the broad range antimicrobial efficacy of linalool. The in vitro effect of linalool on biofilm was quantified in pre-treatment, post-treatment, repetitive treatment, and polymicrobial biofilm scenarios. Time-kill and XTT (2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2 H-tetrazolium-5-carboxanilide) assays were performed to confirm the efficacy of linalool against wound infections, and these results were further validated using simulated wound exudates medium (WEM) which mimics the wound environment. The mechanism of bactericidal action was determined using assays for membrane integrity and oxidative stress. The results indicated the broad range antimicrobial activity of linalool with minimum inhibitory concentration (MIC) ranging from 2.5 to 5 µL/mL against E. coli, A. baumannii, E. faecalis, S. aureus, and K. pneumoniae, while for P. aeruginosa the MIC was 20 µL/mL. Linalool was most effective against E. coli, E. faecalis, K. pneumoniae, A. baumannii, and S. aureus, and could inhibit the growth and biofilm by more than 90% and 80%, respectively, at 5 µL/mL. The XTT assay confirmed the MIC results, showing a significant reduction in the metabolic activity of the pathogens (p < 0.001). In the simulated WEM similar response of the bacteria to linalool treatment was observed. At 5 to 20 µL/mL concentrations, linalool significantly inhibited the polymicrobial biofilm consisting of P. aeruginosa, A. baumannii, and S. aureus in two species combinations. The mechanism of bactericidal action was associated with the increased reactive oxygen species production and disruption in the membrane integrity leading to release of cellular content. The anti-inflammatory activity of linalool, assessed using the albumin denaturation method showed significant activity at the tested concentrations. In conclusion, the findings suggest the therapeutic potential of linalool in treating biofilm associated chronic wound infections due to its versatile broad spectrum activity.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"99"},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular profiling and bioinformatics approaches of biofilm formation in ionizing radiation-resistant Bacillus subtilis, isolated from geothermal spring in Ramsar, the North of Iran.","authors":"Dhuha Saeed Ali, Reza Vazifehmand, Maysoon Abulwahid Malik, Yaya Rukayadi, Son Radu, Mirsasan Mirpour, Mahmud Ab Rashid Nor-Khaizura","doi":"10.1007/s11274-025-04307-9","DOIUrl":"10.1007/s11274-025-04307-9","url":null,"abstract":"<p><p>Biofilm formation and its molecular signaling in bacteria resistant to ionizing radiation is not fully understood. This study aimed to investigate the genetic variations and gene expression of biofilm in an ionizing radiation-resistant Bacillus subtilis in Ramsar. Direct sequencing and quantitative PCR were applied to determine nucleotide variations and gene expression profiles of tapA-sipW-tasA, sinR, sinI, ccpA, epsA-O, spoOB, spoOA, slrA, slrR, ymcA and abrB genes. RNAsnp-RNAfold and Phyre2 and the Swiss Model webserver were used to analyze the structural mRNA and protein respectively. At the molecular level, the tapA-sipW-tasA operon was significantly overexpressed and the expression of ccpA and slrR was significantly downregulated. The thermodynamic and ensemble diversity ratio of the tapA (G>C) gene showed the largest changes in RNA secondary structure. In addition, the largest protein pocket belonged to tapA (148.6 A⁰³) compared to the normal structure (121.1 A⁰³). A non-radiation Bacillus subtilis was served as a control group. These results support the hypothesis that the induction of robust biofilm formation is through the (tapA) operon signal in ionizing radiation-resistant B. subtilis and that genetic variation in tapA (G>C) was the major gene associated with diversity in robust biofilm formation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"97"},"PeriodicalIF":4.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress-tolerant Bacillus strains for enhancing tomato growth and biocontrol of Fusarium oxysporum under saline conditions: functional and genomic characterization.","authors":"María F Valencia-Marin, Salvador Chávez-Avila, Edgardo Sepúlveda, Carmen S Delgado-Ramírez, Jenny J Meza-Contreras, Ma Del Carmen Orozco-Mosqueda, Sergio De Los Santos-Villalobos, Olubukola Oluranti Babalola, Rufina Hernández-Martinez, Gustavo Santoyo","doi":"10.1007/s11274-025-04308-8","DOIUrl":"10.1007/s11274-025-04308-8","url":null,"abstract":"<p><p>Soil salinity is a major limiting factor for agricultural crops, which increases their susceptibility to pathogenic attacks. This is particularly relevant for tomato (Solanum lycopersicum), a salt-sensitive crop. Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici, is a significant threat to tomato production in both greenhouse and field environments. This study evaluated the salinity tolerance, biocontrol, and plant growth-promoting properties of Bacillus velezensis AF12 and Bacillus halotolerans AF23, isolated from soil affected by underground fires and selected for their resistance to saline conditions (up to 1000 mM NaCl). In vitro assays confirmed that both strains produced siderophores, indole-3-acetic acid (IAA), and proteases and exhibited phosphate solubilization under saline stress (100-200 mM NaCl). AF23 exhibited synergistic interactions with AF12, and inoculation with either strain individually or in combination significantly improved the growth of the Bonny Best tomato cultivar under 200 mM saline stress, leading to increased shoot and root weight, enhanced chlorophyll content, and higher total biomass. The biocontrol potential of AF12 and AF23 was evaluated in tomato plants infected with F. oxysporum. Both strains, individually or combined, increased shoot and root weight, chlorophyll content, and total biomass under non-saline conditions, promoting growth and reducing infection rates under saline stress (100 mM NaCl). Genomic analysis revealed that both strains harbored genes related to salt stress tolerance, biocontrol, and plant growth promotion. In conclusion, Bacillus strains AF23 and AF12 demonstrated strong potential as bioinoculants for enhancing tomato growth and providing protection against F. oxysporum in saline-affected soils.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"96"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic microorganisms as an alternative source of thrombolytic compounds: a systematic review.","authors":"Thalya Natasha da Silva Santos, Sara Cadete da Silva, Yanara Alessandra Santana Moura, Marllyn Marques da Silva, Ana Lúcia Figueiredo Porto, Raquel Pedrosa Bezerra","doi":"10.1007/s11274-025-04303-z","DOIUrl":"10.1007/s11274-025-04303-z","url":null,"abstract":"<p><p>Current conventional thrombolytic drugs have some limitations, including a short half-life, several adverse effects, low fibrin specificity, and high cost. Therefore, new thrombolytic sources have been widely investigated worldwide. In this sense, this work aims to evaluate the state of the art of the thrombolytic potential of different bioactive compounds produced from microalgae and cyanobacteria. Then, a systematic literature search was conducted using ScienceDirect, Medline (PubMed), Springer Link, Wiley Online Library, Scielo, MDPI, and BVS electronic databases to select original studies about thrombolytic agents obtained from microalgae and cyanobacteria. After the selection process, 20 studies met the inclusion criteria and were included in the final analysis. Most studies showed promising thrombolytic activity of polysaccharides or proteins produced by cyanobacteria and obtained through homogenization methods. Moreover, the majority of the studies used methods such as activated partial thromboplastin time, prothrombin time, thrombin time, or platelet aggregation tests as parameters to determine the thrombolytic activity. In conclusion, various bioactive compounds from microalgae and cyanobacteria showed high potential to act as alternative thrombolytic therapy, but some characteristics such as mechanism of action, cytotoxicity, immunogenicity and stability parameters need to be more exploited to make the application of these agents feasible in the future.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"95"},"PeriodicalIF":4.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a Na⁺-stimulated acidic hyaluronate lyase from Microbulbifer sp. ALW1 and the antioxidant activity of its hydrolysates.","authors":"Qianli Huang, Chunhua Zhu, Tao Hong, Hebin Li, Lijun Li, Mingjing Zheng, Zhipeng Li, Zedong Jiang, Hui Ni, Yanbing Zhu","doi":"10.1007/s11274-025-04315-9","DOIUrl":"10.1007/s11274-025-04315-9","url":null,"abstract":"<p><p>Hyaluronic acid (HA) is a natural polymer that can be degraded by hyaluronate lyase into oligomers with diverse biological activities. In this study, a novel hyaluronate lyase (named HCLase6) of polysaccharide lyase family 6 from Microbulbifer sp. ALW1 was cloned and characterized. Optimal temperature and pH for HCLase6 was determined to be 40 ℃ and 5.0, respectively. It displayed good stability at temperature up to 45 ℃ and in the pH range of 4.0-9.0. In addition, HCLase6 demonstrated good tolerance to detergents of Tween 20, Tween 80 and SDS, and was halophilic and halotolerant to Na⁺. Molecular dynamics simulations indicated that the presence of Na⁺ increased the flexibility of the loop region adjacent to the active pocket of HCLase6, altered the surface hydrophobicity and electrostatic potential, and strengthened the motion correlation between amino acid residues. Notably, the enzymatic products of HA oligosaccharides (O-HA) produced by HCLase6 showed significantly enhanced free radical scavenging activities and iron reducing power. They also exhibited the antioxidant activity in human keratinocytes cells after exposure to PM SRM 1648a. This study provides the knowledge of the enzymatic properties of HCLase6 and a reference for its industrial application.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"94"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient stress triggers sugar-mediated carotenoid production in algal-bacterial interactions.","authors":"Abdalah Makaranga, Pannaga Pavan Jutur","doi":"10.1007/s11274-025-04310-0","DOIUrl":"10.1007/s11274-025-04310-0","url":null,"abstract":"<p><p>This study examined the impact of co-culturing Chlorella saccharophila (UTEX247) with Exiguobacterium sp. strain AMK1 on carotenoid production under nitrate-depleted conditions and 3% CO₂ supplementation. The co-culture significantly enhanced the productivity of lutein (238.31 µg.L⁻¹d⁻¹), zeaxanthin (220.72 µg.L⁻¹d⁻¹), violaxanthin (185.42 µg.L⁻¹d⁻¹), and antheraxanthin (84.07 µg.L⁻¹d⁻¹). Compared to nitrate-repleted mono-cultures, these carotenoids increased by 3.54-fold, 4.81-fold, 12.28-fold, and 9.34-fold, respectively. The violaxanthin cycle, activated by CO₂ supplementation, resulted in higher zeaxanthin production, verified through HPLC analysis. Metabolic profiling highlighted a notable rise in sucrose, an algal-specific metabolite, in the co-culture, reflecting enhanced carbon metabolism and carotenoid synthesis. Conversely, trehalose levels were significantly higher in the bacterial mono-culture (297.77 µg.mL⁻¹) than in the co-culture (88.84 µg.mL⁻¹), showing a 1.68-fold reduction as confirmed by GC-MS/MS. This suggests trehalose as a stress marker, with its reduction indicating mutualistic interactions between algal and bacterial. Overall, the co-culture strategy emerges as a promising approach to activate unexpressed pathways, generate novel metabolites, and enhance yields of valuable carotenoids like lutein and zeaxanthin. This aligns with the principles of a circular bioeconomy, leveraging bacterial biofertilizers, valorizing CO₂, and minimizing chemical dependency, thus offering potential for biorefinery applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"93"},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in Escherichia coli secretion systems for enhanced recombinant protein production.","authors":"Sudarsana Reddy Lokireddy, Sridhar Rao Kunchala, Ramakrishna Vadde","doi":"10.1007/s11274-025-04302-0","DOIUrl":"10.1007/s11274-025-04302-0","url":null,"abstract":"<p><p>Escherichia coli is inarguably one of the most studied microorganisms across the spectrum of microbiology. It is very widely used in recombinant protein production owing to its rapid growth, ease of genetic manipulation, and relatively high protein yields. Despite all of its advantages, its inability to efficiently secrete proteins naturally remains a drawback leading to protein aggregation as inclusion bodies in the cytoplasm and consequent low overall protein yield. Therefore, many approaches to mitigate this weakness and enhance extracellular secretion to increase protein yield have been devised. This review explores the natural and engineered secretion systems in E. coli, highlighting their potential for enhanced protein secretion for non-glycosylated proteins. Natural one-step (e.g., Type I and III Secretion Systems) and two-step systems (e.g., Sec and Tat pathways) are detailed alongside recent advancements in genetic engineering, mutagenesis, and synthetic biology approaches aimed at improving protein yield, folding, and secretion efficiency. Emerging technologies, such as the ESETEC^® and BacSec^® platforms, promise scalable and cost-effective solutions for higher protein production. Challenges, including limited cellular capabilities and protein aggregation, are addressed through innovative strategies like cell wall modification, co-expression of chaperones, and medium optimization. This review emphasizes E. coli's adaptability to industrial applications, and the promising future of recombinant protein technologies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"90"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the microbial community dynamics and metabolic pathways of primary organic acids in Sichuan Baoning vinegar through metagenomics.","authors":"Aiping Liu, Jie Wu, Jianlong Li, Qin Li, Ning Zhao, Kaidi Hu, Shuliang Liu, Giuseppe Blaiotta, Jiang Zhou","doi":"10.1007/s11274-025-04306-w","DOIUrl":"10.1007/s11274-025-04306-w","url":null,"abstract":"<p><p>Sichuan Baoning vinegar, a renowned traditional vinegar in China, exhibits a higher lactic acid content compared to acetic acid. The microbiota plays a crucial role in shaping the unique flavor of vinegar, but the species-level succession of key microorganisms and metabolic pathways of major organic acids in this vinegar are still unclear. This study utilized metagenomic sequencing to elucidate microbial succession during fermentation and the functional roles of the microbial community, as well as explore the metabolic network of lactic acid and acetic acid. Our findings revealed that bacteria dominated the fermentation process, with Acetilactobacillus jinshanensis, Lactobacillus amylovorus, and Limosilactobacillus sp. emerging as the top three species. Notably, Acetilactobacillus jinshanensis, Limosilactobacillus sp., Lactobacillus amylovorus, and Limosilactobacillus pontis were key players in lactic acid production, while acetic acid synthesis might be primarily driven by Lactobacillus amylovorus, Limosilactobacillus sp., Lactobacillus acetotolerans, and Acetobacter pasteurianus. This study enhances our understanding of the key microorganisms and organic acids metabolism in vinegar, shedding light on the fermentation mechanism of cereal vinegar.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"91"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of pre-treatments for cost effective production of bioethanol from floral wastes.","authors":"Shalini Rachel, M Shailaja Raj, Maria Shajan","doi":"10.1007/s11274-025-04305-x","DOIUrl":"10.1007/s11274-025-04305-x","url":null,"abstract":"<p><p>The present study has focused on validating pre-treatment methods for cost effective production of bioethanol from discarded and otherwise waste flowers which are renewable, abundantly available and eco-friendly. Floral waste was collected from various dumpsites and banquet halls and subjected to physical, chemical and biological pre-treatments. Biological pre-treatment by enzymatic hydrolysis using crude cellulase enzyme (5%) yielded 39.4 ± 0.03 g/L of alcohol which is 24.20 and 31.60% more than the alcohol obtained by physical pre-treatment (thermal hydrolysis) and chemical pre-treatment (1% KOH), therefore simultaneous saccharification and fermentation was optimised. A maximum of 396 ± 6.48 g/L bioethanol was obtained after 96 h of fermentation with the isolated yeast, Pichia kudriavzevii CY 902 at pH 5.5 and 37 °C. The minimum ethanol selling price (MESP) of bioethanol produced in our study was enumerated to be 30.43 Rs/L which is 68.31% lesser than the market price of ethanol in India today, making our methodology for production of bioethanol from mixed floral wastes very competitive and cost effective to the existing methodologies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"92"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}