World journal of microbiology & biotechnology最新文献

{"title":"Advancements in Escherichia coli secretion systems for enhanced recombinant protein production.","authors":"Sudarsana Reddy Lokireddy, Sridhar Rao Kunchala, Ramakrishna Vadde","doi":"10.1007/s11274-025-04302-0","DOIUrl":"10.1007/s11274-025-04302-0","url":null,"abstract":"<p><p>Escherichia coli is inarguably one of the most studied microorganisms across the spectrum of microbiology. It is very widely used in recombinant protein production owing to its rapid growth, ease of genetic manipulation, and relatively high protein yields. Despite all of its advantages, its inability to efficiently secrete proteins naturally remains a drawback leading to protein aggregation as inclusion bodies in the cytoplasm and consequent low overall protein yield. Therefore, many approaches to mitigate this weakness and enhance extracellular secretion to increase protein yield have been devised. This review explores the natural and engineered secretion systems in E. coli, highlighting their potential for enhanced protein secretion for non-glycosylated proteins. Natural one-step (e.g., Type I and III Secretion Systems) and two-step systems (e.g., Sec and Tat pathways) are detailed alongside recent advancements in genetic engineering, mutagenesis, and synthetic biology approaches aimed at improving protein yield, folding, and secretion efficiency. Emerging technologies, such as the ESETEC^® and BacSec^® platforms, promise scalable and cost-effective solutions for higher protein production. Challenges, including limited cellular capabilities and protein aggregation, are addressed through innovative strategies like cell wall modification, co-expression of chaperones, and medium optimization. This review emphasizes E. coli's adaptability to industrial applications, and the promising future of recombinant protein technologies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"90"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the microbial community dynamics and metabolic pathways of primary organic acids in Sichuan Baoning vinegar through metagenomics.","authors":"Aiping Liu, Jie Wu, Jianlong Li, Qin Li, Ning Zhao, Kaidi Hu, Shuliang Liu, Giuseppe Blaiotta, Jiang Zhou","doi":"10.1007/s11274-025-04306-w","DOIUrl":"10.1007/s11274-025-04306-w","url":null,"abstract":"<p><p>Sichuan Baoning vinegar, a renowned traditional vinegar in China, exhibits a higher lactic acid content compared to acetic acid. The microbiota plays a crucial role in shaping the unique flavor of vinegar, but the species-level succession of key microorganisms and metabolic pathways of major organic acids in this vinegar are still unclear. This study utilized metagenomic sequencing to elucidate microbial succession during fermentation and the functional roles of the microbial community, as well as explore the metabolic network of lactic acid and acetic acid. Our findings revealed that bacteria dominated the fermentation process, with Acetilactobacillus jinshanensis, Lactobacillus amylovorus, and Limosilactobacillus sp. emerging as the top three species. Notably, Acetilactobacillus jinshanensis, Limosilactobacillus sp., Lactobacillus amylovorus, and Limosilactobacillus pontis were key players in lactic acid production, while acetic acid synthesis might be primarily driven by Lactobacillus amylovorus, Limosilactobacillus sp., Lactobacillus acetotolerans, and Acetobacter pasteurianus. This study enhances our understanding of the key microorganisms and organic acids metabolism in vinegar, shedding light on the fermentation mechanism of cereal vinegar.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"91"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of pre-treatments for cost effective production of bioethanol from floral wastes.","authors":"Shalini Rachel, M Shailaja Raj, Maria Shajan","doi":"10.1007/s11274-025-04305-x","DOIUrl":"10.1007/s11274-025-04305-x","url":null,"abstract":"<p><p>The present study has focused on validating pre-treatment methods for cost effective production of bioethanol from discarded and otherwise waste flowers which are renewable, abundantly available and eco-friendly. Floral waste was collected from various dumpsites and banquet halls and subjected to physical, chemical and biological pre-treatments. Biological pre-treatment by enzymatic hydrolysis using crude cellulase enzyme (5%) yielded 39.4 ± 0.03 g/L of alcohol which is 24.20 and 31.60% more than the alcohol obtained by physical pre-treatment (thermal hydrolysis) and chemical pre-treatment (1% KOH), therefore simultaneous saccharification and fermentation was optimised. A maximum of 396 ± 6.48 g/L bioethanol was obtained after 96 h of fermentation with the isolated yeast, Pichia kudriavzevii CY 902 at pH 5.5 and 37 °C. The minimum ethanol selling price (MESP) of bioethanol produced in our study was enumerated to be 30.43 Rs/L which is 68.31% lesser than the market price of ethanol in India today, making our methodology for production of bioethanol from mixed floral wastes very competitive and cost effective to the existing methodologies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"92"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic review and meta-analysis of the potential effects of endophytic bacteria Klebsiella on plant growth promotion and biocontrol of pathogens.","authors":"Odyone Nascimento da Silva Bandeira, Renato da Silva Bandeira, Cláudia Regina Batista de Souza","doi":"10.1007/s11274-025-04300-2","DOIUrl":"10.1007/s11274-025-04300-2","url":null,"abstract":"<p><p>Klebsiella is a bacterial genus widely recognized in the medical field but with underexplored potential in agriculture. This study employed a systematic review and meta-analysis to investigate scientific articles on plant growth promotion effects associated with endophytic bacteria Klebsiella species. A total of 39 relevant studies, published between 2012 and 2024, were identified based on strict inclusion and exclusion criteria. The analysis revealed that Klebsiella sp., K. pneumoniae, and K. variicola are cosmopolitan species that have functional versatility in phytohormone production, nutrient solubilization, and pathogen control in agricultural systems in both tropical and temperate zones. The data showed a significant correlation between the use of Klebsiella sp. and plant growth, highlighting the positive impact of these species in controlling aggressive pathogens. These findings underscore the potential of Klebsiella as a biotechnological tool for sustainable agricultural practices, enhancing plant growth and reducing the reliance on chemical inputs. The study further emphasizes the need for future research to deepen genomic characterization and expand the agricultural applications of these bacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"89"},"PeriodicalIF":4.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting disease resistance in Solanum melongena L. (eggplant) against Alternaria solani: the synergistic effect of biocontrol Acinetobacter sp. and indole-3-acetic acid (IAA).","authors":"Mohammad Danish, Mohammad Shahid, Zaryab Shafi, Mohammad Tarique Zeyad, Mohammad Abul Farah, Khalid Mashay Al-Anazi, Lukman Ahamad","doi":"10.1007/s11274-025-04282-1","DOIUrl":"10.1007/s11274-025-04282-1","url":null,"abstract":"<p><p>Alternaria solani causes early blight disease in eggplants, threatening production and leading to significant economic losses. Fungicides are used to control fungal diseases, but their overuse raises resistance concerns. Finding novel, eco-friendly biocontrol agents is therefore a solution for the future. The coordination between antagonistic bacterial agents and plant growth hormones in defense responses against fungal pathogens are crucial. This study assessed biocontrol potential of Acinetobacter sp. SCR-11 (Accession no. OR751536.1) and indole-3-acetic acid (IAA; 100 µM), singly and in combination, against A. solani in eggplants. Strain SCR-11 produced hydrogen cyanide (HCN; 5.7 µg mL⁻¹), siderophore i.e. salicylic acid (14.7 µg mL⁻¹), 2,3-dihydroxybenzoic acid (23.1 µg mL⁻¹) and various extracellular lytic enzymes. Strain SCR-11 exhibited antagonistic activity by strongly inhibiting (82%) A. solani. Acinetobacter sp. inoculation and IAA treatment enhanced growth, biomass, and leaf pigments of A. solani-diseased eggplants, with effectiveness in order: SCR-11 + IAA > SCR-11 > IAA >. The combined treatments (SCR-11 + IAA) most effectively increased total soluble protein (62.5%), carbohydrate (60%), total soluble sugar (81%), and phenol (74%) in A. solani-infected eggplant. Biocontrol agent and IAA application significantly (p ≤ 0.05) reduced proline and malondialdehyde (MDA) levels, alleviating oxidative stress in A. solani-diseased eggplant. The SCR-11 + IAA treatment significantly reduced the percent disease index (71%) and increased protection (69%) in diseased eggplant. The Acinetobacter sp. and IAA coordination enhanced disease resistance in A. solani-infected eggplants by boosting defense enzyme activities (SOD, POD, PAL, and β-1, 3 glucanase), significantly protecting plants from pathogen attack. At harvest, soil populations of A. solani decreased, while SCR-11 populations increased significantly. Acinetobacter sp. and IAA work synergistically through pathogen suppression, plant growth promotion, and induction of plant defense responses. Thus, applying antagonistic PGPR strain with exogenous IAA enhances eggplant resistance to A. solani, providing an environmentally friendly agricultural solution.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"85"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable microbial solutions for managing fungal threats in wheat: progress and future directions.","authors":"Prem Lal Kashyap, Sudheer Kumar, Annie Khanna, Poonam Jasrotia, Gyanendra Singh","doi":"10.1007/s11274-025-04286-x","DOIUrl":"10.1007/s11274-025-04286-x","url":null,"abstract":"<p><p>Biotrophic and necrotrophic fungi are responsible for causing a range of diseases in wheat, resulting in significant economic losses and a decline in quality. Effective management of these diseases generally involves a combination of resistance breeding, chemical treatments, and cultural practices. However, traditional breeding methods have made limited progress due to the slow pace of genetic improvements, the complexity of the wheat genome, and the quantitative nature of disease resistance traits, along with the constantly evolving virulence of pathogens. This situation has prompted research into more effective and eco-friendly alternatives, such as biological control. Recent studies have concentrated on using antagonistic microbes to decrease the reliance on chemical pesticides while enhancing wheat health and productivity. A comprehensive overview of current knowledge on wheat disease outbreaks is being developed, with a focus on advancements in biological control strategies. The review will first discuss the key fungal pathogens and their associated diseases, followed by a summary of biological control methods, particularly emphasizing potential microbial antagonists. Additionally, it will explore strategies to improve the efficacy of biocontrol agents, which are crucial for a holistic and sustainable approach to wheat disease management. Ultimately, the article will highlight the role of biological control in promoting more sustainable agricultural practices, particularly concerning wheat diseases, in alignment with the UN sustainable development goals.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"79"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Underlying the early signaling and gene expression of Pleurotus ostreatus mycelia during injury response.","authors":"Jiachun Zhu, Jiaqi Sun, Huiping Li, Ping Xu, Lin Ma, Qiang Wang, Keping Chen, Shaoxuan Qu","doi":"10.1007/s11274-025-04296-9","DOIUrl":"10.1007/s11274-025-04296-9","url":null,"abstract":"<p><p>Plants and animals have demonstrated conserved damage defense systems, but the injury response mechanisms in fungi remain largely unexplored. In this study, we investigate the early defensive mechanisms activated by mechanical damage in the basidiomycota Pleurotus ostreatus. Assays of defense enzymes and gene expression in P. ostreatus revealed the role of reactive oxygen species (ROS) and the activation of the mitogen-activated protein kinase (MAPK) signaling pathway in this process. The findings from the defense-related enzyme tests showed that damage enhanced NAD/NADPH-dependent ROS production and endogenous carbon monoxide expression. Additionally, qRT-PCR data provided further evidence for H₂O₂ generation, highlighting the involvement of MAPK signaling and several early defense components in the damage response of P. ostreatus. Notably, there were differences in the damage response among various pest-resistant strains, with several genes specifically expressed in the pest-resistant strain. Pretreatment of damaged mycelium with N, N'-dimethylthiourea (DMTU, a hydroxyl radical scavenger), diphenyleneiodonium chloride (DPI, a NADPH oxidase inhibitor), or BAPTA (a calcium chelator) significantly inhibited hyphal regeneration and growth. These findings also imply that Ca²⁺ was an another important signaling molecule in response to wounding. This study provides a more comprehensive holistic view on the damage response of P. ostreatus, and offers valuable references for future research into its biological traits and prospective uses.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"80"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in the cyanobacterial products and applications of phycocyanins.","authors":"Jinichi Aoki, Runa Koshikawa, Munehiko Asayama","doi":"10.1007/s11274-025-04297-8","DOIUrl":"10.1007/s11274-025-04297-8","url":null,"abstract":"<p><p>Recent developments in the research on cyanobacterial products have drawn increasing attention, especially in the production and application of phycocyanin, which has shown great potential in various fields. Cyanobacteria are photosynthetic prokaryotes that live on Earth and are the ancestors of plant chloroplasts. They have a compact genome size compared to other eukaryotic photosynthesizing microorganisms; some species are genetically engineered and have high growth potential in indoor culture, and some strainscan maintain high growth potential even in outdoor culture. Cyanobacteria are valuable because they can selectively and effectively produce and recover useful substances that are poorly produced by other microalgae, although this depends on the algal species. However, the social implementation of biorefineries using cyanobacteria involves issues such as setting up useful products in addition to the culture methods and strains to be used. This review aims to present research trends over the last 20 years on the production of useful substances such as biodegradable plastics, lipids, polysaccharides, and pigment proteins (phycocyanins) from cyanobacteria. Phycocyanin is mainly recovered and purified by filamentous cyanobacteria and has contributed to the research field, especially in the food and beverage industry. Additionally, the production and functions of phycocyanin are summarized to provide a better understanding of these possibilities. Their potential applications as environmentally friendly materials are also described to further contribute to the research field and social implementation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"84"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in fluorescent labeling in assessing the probiotic adhesion capacity - A review.","authors":"Weiping Xu, Han Tan, Peiling Hu, Shijian Liu, Jiajia Song, Xiaoyong Chen, Huayi Suo","doi":"10.1007/s11274-024-04186-6","DOIUrl":"10.1007/s11274-024-04186-6","url":null,"abstract":"<p><p>Adhesion capacity of probiotics is closely related to their intestinal-protective effects. The conventional techniques used to evaluate probiotic adhesion capacity have limitations in terms of imaging resolution and quantitative analysis. Fluorescent labelling technology has shown immense potential in recent years owing to its high specificity and sensitivity for resolving probiotic adhesion mechanisms. Although there are still problems with the fluorescence signal intensity and hysteresis effect, this technology has significantly advanced the accurate detection and evaluation of probiotic adhesion capacity. This review examines the critical role of probiotic adhesion and its detection methods, with a special focus on the application of fluorescent-labeling technology. Our objective was to identify more accurate and efficient approaches for evaluating the adhesion capacity of probiotic bacteria while promoting in-depth research into the underlying mechanisms that govern probiotic adhesion.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"73"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amphibian skin bacteria contain a wide repertoire of genes linked to their antifungal capacities.","authors":"Francisco González-Serrano, Yordan J Romero-Contreras, Alberto H Orta, M Delia Basanta, Hugo Morales, Gabriela Sandoval García, Elena Bello-López, A S Escobedo-Muñoz, Víctor H Bustamante, Víctor Ávila-Akerberg, Miguel Ángel Cevallos, Mario Serrano, Eria A Rebollar","doi":"10.1007/s11274-025-04292-z","DOIUrl":"10.1007/s11274-025-04292-z","url":null,"abstract":"<p><p>Emerging diseases caused by fungi are a serious threat to wildlife biodiversity. The widespread fungal pathogen Batrachochytrium dendrobatidis (Bd) has caused dramatic amphibian population declines and species extinctions worldwide. While many amphibians have been negatively affected by Bd, some populations/species have persisted despite its presence. One factor contributing to amphibian protection against this fungus is the host-associated skin microbiome. In this study, we aimed to identify gene clusters associated with the antifungal activity of amphibian skin bacteria. Specifically, we explored skin bacteria isolated from species that have persisted in the wild despite the presence of Bd: the frogs Agalychnis callidryas, Craugastor fitzingeri, Dendropsophus ebraccatus, and the axolotl Ambystoma altamirani. Bacterial isolates were tested in vitro for their capacity to inhibit the growth of two fungal pathogens: Bd and the phytopathogen Botrytis cinerea (Bc). Genome mining of these bacterial isolates revealed a diverse repertoire of Biosynthetic Gene Clusters (BGCs) and chitin-degrading gene families (ChDGFs) whose composition and abundance differed among bacterial families. We found specific BGCs and ChDGFs that were associated with the capacity of bacteria to inhibit the growth of either Bd or Bc, suggesting that distinct fungi could be inhibited by different molecular mechanisms. By using similarity networks and machine learning, we identified BGCs encoding known antifungal compounds such as viscosin, fengycin, zwittermicin, as well as siderophores and a novel family of beta-lactones. Finally, we propose that the diversity of BGCs found in amphibian skin bacteria comprise a substantial genetic reservoir that could collectively explain the antifungal properties of the amphibian skin microbiome.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"78"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}