World journal of microbiology & biotechnology最新文献

{"title":"Genome-wide identification of the lysine deacetylases gene and its dynamic expression profile during adversity stress and infestation in Arthrinium phaeospermum.","authors":"Sijia Liu, Ziqi Ye, Jia Song, Yutong Liu, Shujiang Li","doi":"10.1007/s11274-025-04479-4","DOIUrl":"10.1007/s11274-025-04479-4","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"263"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microcella aerolata GA224 exhibits preventive potential against Streptococcus pneumoniae infection via the gut-lung axis.","authors":"Ningqianzi Tang, Yimin Pan, Zicheng Jin, Guoxia Zhang","doi":"10.1007/s11274-025-04478-5","DOIUrl":"10.1007/s11274-025-04478-5","url":null,"abstract":"<p><p>Antibiotics and vaccines have long been major key interventions against Streptococcus pneumoniae (Spn) infection. However, alternative therapies are urgently needed with the original therapies becoming suboptimal efficacy. A beneficial bacterium, Microcella aerolata strain GA224, with protective potential against Spn infection was isolated in previous study. Here, this protective effect was investigated at the bacterial, cellular and animal levels, exploring the mechanisms from the perspective of the gut-lung axis. Cellular and animal models of Spn infection were established and GA224 was administered for prevention or treatment. Spn adherence, inflammatory gene expression, histopathological features, gut microbial profiles and fecal metabolomic signatures were examined. In vitro, GA224 inhibited Spn growth with a bacteriostatic diameter of 16 mm and reduced adherence by 83.4% (P < 0.01) in prevention groups, while suppressing inflammatory gene expression (IL-1β, IL-6, TNF-α) by 40-50% (P < 0.001) in Spn-infected cells. In Spn-infected rats, GA224 intranasal administration improved body weight gain by 8.2% (vs. Tre group + 3.7%, P < 0.05) and reduced lung injury score by 25% (P < 0.01). In addition, GA224 administration alleviated dysbiosis of the gut microbiota, including declined abundance of Ruminiclostridium and Roseburia, increased abundance of Ruminococcus and restricted out-migration of infectious bacteria. In terms of the fecal metabolism, differential metabolites and disordered metabolic pathways were altered by GA224, including glycine, serine and threonine metabolism and pentose and glucuronate interconversion. Intestinal bacteria showed multiple correlations with fecal metabolites with strongest correlation founding between Ruminococcus and oxidized phospholipids. Notably, Anaerotaenia (r = -0.67 with LysoPC), Ruminiclostridium (positively correlated with lung coefficient, r = 0.62) and Ruminococcus (strongest correlation with oxidized phospholipids, r = 0.8) demonstrated microbiota-metabolite interactions potentially mediating gut-lung axis regulation.Finally, we demonstrate that M. aerolata GA224 provided a protective potential against Spn infection. In addition to the inhibition in adherence and inflammation, remodeling gut microbiota and improving metabolism via the gut-lung axis may be the critical avenue to this protective effect.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"259"},"PeriodicalIF":4.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Shiga toxin-producing Escherichia Coli O157 in Moroccan food and antimicrobial resistance profiles of the strains isolated.","authors":"Bouchra Ouarroud, Mohammed El Maadoudi, Lamyaa El Mamoun, Ayoub Kounnoun, Amina Barakat","doi":"10.1007/s11274-025-04477-6","DOIUrl":"10.1007/s11274-025-04477-6","url":null,"abstract":"<p><p>Foods contaminated with Shiga toxin-producing Escherichia coli (STEC) are implicated in serious outbreaks. The present study investigated the prevalence of stx1, stx2, eae, and rfbEO157 genes by real-time PCR in enrichment broths of ground beef, artisanal dairy products, lettuce, spinach, turkey, chicken and bivalve mollusks sold in northern Morocco, the isolation of STEC O157 from positive enrichment broths and determination of antimicrobial resistance profiles of the isolates. The screening results revealed that amongst 620 samples analyzed, 82 enrichment broths (13.23%) were stx + (stx1 or/and stx2) eae- rfbEO157-, 77 out of 620 enrichment broths (12.42%) were stx + eae + rfbEO157-, and 73 out of 620 enrichment broths (11.77%) were stx + eae + rfbEO157 + . The food matrix most contaminated was ground beef 33/140 (23.57%), followed by dairy 27/140 (19.29%), poultry 8/140 (5.71%), leafy vegetables 4/100 (4.00%) and then bivalve mollusks 1/100 (1.00%). 14 STEC O157 isolates were isolated from 4 positive samples (4/73; 5.5%) (multiple isolates were recovered from each positive sample). Antibiotic susceptibility testing showed that the STEC O157 isolates were resistant to at least 4 of the 20 antimicrobials tested. All isolates were resistant to amoxicillin/clavulanic acid, 11/14 (78.6%) were resistant to streptomycin, 10/14 (71.4%) were resistant to tobramycin, 9/14 (64.3%) were resistant to ertapenem and piperacillin, 8/14 (57.1%) were resistant to cefotaxime and 7/14 (50.0%) were resistant to ampicillin and ceftazidime. All isolates were sensitive to amikacin, ceftazidime/avibactam, gentamicin, imipenem, nitrofurantoin and trimethoprim/sulfamethoxazole. Results indicate food producers and sellers may need further training in good hygiene rules, highlighting the importance of implementing strict quality control to ensure food safety.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"258"},"PeriodicalIF":4.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of aflatoxin in dried figs.","authors":"Senem Öztürk Köse, Hacı Halil Biyik","doi":"10.1007/s11274-025-04466-9","DOIUrl":"10.1007/s11274-025-04466-9","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"257"},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity, metabolic properties, and biotechnological prospects of acid-sulfate soils.","authors":"Mavi Johanna Blanco-Diaz, Valéria Maia Oliveira","doi":"10.1007/s11274-025-04444-1","DOIUrl":"10.1007/s11274-025-04444-1","url":null,"abstract":"<p><p>Acid-sulphate soils (ASS) are worldwide naturally or anthropogenically formed soils that originate from the oxidation of pyrite in anoxic sulfide substrates. Classification of soils into ASS types is complex and usually depends on grain size, oxidation rates, pH values, acidification potential and typical microbial communities. Currently, there is not a consensual classification of ASS and definitions are not universally applied. The global use of ASS for crops and mining areas has become an environmental problem, raising concerns in the scientific community and prompting the study of microorganisms inhabiting this acidic environment and their potential for further use in bioremediation processes. Globally, ASS harbor a higher abundance of the bacterial phylum Pseudomonadota, especially members of the Beta and Gammaproteobacteria classes, followed by other less abundant phyla like Bacillota, Actinomycetota, Acidobacteriota, Bacteroidetes, Nitrospirota and Chloroflexota. Among the archaea reported in ASS are members of the genera Thermoproteus and Acidilobus and of the Thermoplasmata class. Bacteria and archaea found in the different types of ASS exhibit diverse metabolisms, including dissimilatory processes like iron and/or inorganic sulfur oxidation, iron or sulfate reduction, aluminum and iron reduction and methanogenesis, assimilatory processes like autotrophy, diazotrophy, and other metabolic properties such as phosphate solubilization and acidogenesis. This diversity offers a wide range of biotechnological applications, including bioremediation of crop areas and natural ecosystems affected by acidity and a vast field for basic and applied scientific research.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"256"},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monochromatic LeafAdaptNet (MLAN): an adaptive approach to spinach leaf disease detection using monochromatic imaging.","authors":"Meganathan Elumalai, Terrance Frederick Fernandez, R Kaviarasan, S Kannadhasan","doi":"10.1007/s11274-025-04442-3","DOIUrl":"10.1007/s11274-025-04442-3","url":null,"abstract":"<p><p>A country's economic growth heavily relies on agricultural productivity, specifically nutrition derived from vegetables and leafy greens. Spinach, abundant in iron, vitamins, and other essential nutrients, plays a vital role in maintaining the health of human tissues, cartilage, and hair. However, extreme summer heat and plant diseases can significantly reduce spinach yields, making it less nutritious and harder to obtain. Implementing improved detection and classification of bacterial and fungal diseases affecting spinach leaves is crucial for minimizing pesticide use and enhancing agricultural output. A cutting-edge approach was introduced for identifying diseases in spinach leaves through deep learning object detection. To tackle these issues, the DenseNet-121-DO model served as the basis for developing the Custom Monochromatic LeafAdaptNet (MLAN). Spinach leaves were classified as Half-Spinach, Curry Leaves, Drumstick Leaves, and Lettuce, with the aid of Google-Colaboratory. This model displayed impressive results, achieving an accuracy of 99.10% and a mean Average Precision (mAP) of 98.16%. Such outcomes promote higher agricultural productivity and reduced pesticide costs by showcasing the system's effectiveness in accurately identifying and classifying spinach leaf diseases.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"255"},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic changes in Phycomyces blakesleeanus mycelia during selenite reduction and cellular localization of synthesized SeNPs.","authors":"Ivanka Rodić, Milan V Žižić, Jovana Lukičić, Marina Stanić, Alessandra Gianoncelli, Valentina Bonanni, Joanna Zakrzewska, Miroslav Ž Živić, Tijana Cvetić Antić","doi":"10.1007/s11274-025-04416-5","DOIUrl":"10.1007/s11274-025-04416-5","url":null,"abstract":"<p><p>This study considers the capacity of fungus Phycomyces blakesleeanus for removal of toxic selenite from the environment and metabolic response of the mycelium during Se transformation. The X-ray Fluorescence Microscopy (XFM) suggests two pathways of selenite transformation: extensive internalisation and intracellular transformation leading to volatilization, and synthesis of SeNPs at the cell surface, with the contribution of each pathway depending on selenite concentration and treatment duration. Glutathione plays an important role in the reduction process, as the glutathione pool is alternately removed from and restored to redox balance during Se treatment. Enzymes facilitate the maintenance of the cellular redox balance, which is reflected in an increase in the specific activities of glutathione reductase, glutathione peroxidase, glutathione S-transferase and catalase at different time points during the 48-h exposure of mycelia to 100 µM selenite. During the transition from the exponential to the stationary growth phase, a metabolic shift was documented, which can be seen in the change of the total glutathione content and glutathione redox status. This points out that the developmental stage of the mycelia plays an important role in the capacity for selenite reduction and mycelia survival in a selenium-enriched medium. This work is a step towards the use of selenite-contaminated media for Se extraction and re-utilisation and suggests that Phycomyces blakesleeanus might be a suitable organism for the effective re-utilisation of Se.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"254"},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the impact of Curvularia pathogens on medicinal and aromatic plants: insights into history, pathogenicity, and host-pathogen interactions.","authors":"Atul Kumar Srivastava, Mehran Khan, Xiangyang Li, Pooja Misra, Ashish, Akhil Kumar, Entaj Tarafder, Xin Xie, Raghvendra Pratap Singh","doi":"10.1007/s11274-025-04421-8","DOIUrl":"10.1007/s11274-025-04421-8","url":null,"abstract":"<p><p>The genus Curvularia comprises globally distributed phytopathogenic fungi that predominantly infect grasses, including various Medicinal and Aromatic Plants (MAPs). Despite its ecological and agricultural significance, studies on the morphology, phenotypic diversity, and infection mechanisms of Curvularia in MAPs remains scarce. Taxonomic understanding of this genus has also evolved over time, with repeated revisions and reclassifications. This review presents a chronicle key discovery, taxonomic updates, and systematic advancements in Curvularia research. Known to severely impact the Poaceae family, staple crops, and MAPs (Rauvolfia, Ocimum spp., Aloe vera, and Curcuma spp.) Curvularia employ diverse genes to disrupt host metabolic pathways, inducing physiological dysfunctions disease symptoms. Here, we consolidate global reports on Curvularia-associated diseases in MAPs, analysing host-pathogen interactions across geographical regions. Furthermore, we explore the disease cycle, biochemical infection strategies, and molecular pathogenesis to elucidate fungal virulence. A dedicated focus is placed on Curvularia-derived secondary metabolites and toxins, highlighting their role in host manipulation and pathogenicity. By integrating these insights, this review aims to address critical knowledge gaps and direct future research on Curvularia-MAPs interactions.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"252"},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification.","authors":"Yashika Raheja, Varinder Singh, Vivek Kumar Gaur, Gaurav Sharma, Adrian Tsang, Bhupinder Singh Chadha","doi":"10.1007/s11274-025-04473-w","DOIUrl":"10.1007/s11274-025-04473-w","url":null,"abstract":"<p><p>GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 could fill this gap and how it stacks up against the industrial workhorse strain Trichoderma reesei Cel7A, we codon optimized the R. emersonii gene (Rem_GH7CBHI), expressed it in Pichia pastoris and purified the recombinant enzyme for structural and functional analysis. The 57 kDa protein retains the canonical GH7 βsandwich tunnel, but an AlaforTyr substitution leaves the channel more open than that of T. reesei, potentially easing substrate entry. Consistent with this architecture, Rem_GH7CBHI binds cellotriose tightly and exhibits a low Kₘ of 0.25 mM. Biochemical characterization revealed the optimal activity at pH 5.0, 60 °C and retaining about 60% activity after 1 h at 70 °C. Adding Rem_GH7CBHI together with an endogenous AA9 LPMO to the native R. emersonii secretome (M36) boosted saccharification of steamacidpretreated rice straw to levels comparable with commercial Cellic CTec3 at the same total protein loading. These results position Rem_GH7CBHI as a thermostable, highaffinity alternative to T. reesei Cel7A and a costeffective addition to tailored enzyme cocktails for highsolids lignocellulose biorefineries.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"253"},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of microbial community structures in rhizosphere soils of different plants in riverine wetland.","authors":"Jiaying Zhao, Biao Liu, Yiran Hou, Liujie Zheng, Xixi Kong, Changrui Zhou, Junfeng Wu, Xiang Guo, Yimei Ying","doi":"10.1007/s11274-025-04423-6","DOIUrl":"10.1007/s11274-025-04423-6","url":null,"abstract":"<p><p>Bacterial communities play an important role in nutrient cycling in riverine wetland ecosystems, and the study of rhizosphere microbial community composition of different plants is of great scientific significance in revealing the relationship between microorganisms and plant interactions in riverine wetlands. In this study, samples were collected from four plant congeries dominated by Artemisia argyi (AH), Acorus calamus (CP), Miscanthus sacchariflorus (DC), and Paspalum distichum (QB), respectively. High-throughput sequencing technology was employed to investigate the rhizosphere microbial community structures associated with these distinct plant species. The results showed that soil water content could effectively regulate the functional diversity of soil microbial communities. Different plant congeries types significantly affected soil bacterial community diversity, and at the phylum level, both Proteobacteria and Acidobacteria were absolutely dominant groups, followed by Bacteroidetes. At the genus level, Flavobacterium, Sphingomonas, Gp6 and Gp4 were the dominant bacteria in the rhizosphere soil of plants along the river. Soil pH, water content, NO₃-N, NO₂-N and TOC were the main factors affecting the composition of microbial communities. This study broadens our understanding of the composition of rhizosphere microbial communities in the river buffer zone, and provide data support for river ecological environment protection.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"250"},"PeriodicalIF":4.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}