World journal of microbiology & biotechnology最新文献

{"title":"CRISPR/Cas9-mediated genome editing in Ganoderma lucidum: recent advances and biotechnological opportunities.","authors":"Dongmei Lv, Yan Xu, Zi-Xu Wang, Qi-Lin Zhang, Jin-Ping Yan, Jun-Wei Xu","doi":"10.1007/s11274-025-04458-9","DOIUrl":"https://doi.org/10.1007/s11274-025-04458-9","url":null,"abstract":"<p><p>Ganoderma lucidum is a well-known traditional medicinal mushroom that has attracted considerable attention due to its potential as a promising cell factory for producing high-value bioactive compounds. However, conventional methods for the genetic manipulation of G. lucidum are often time-consuming and labor-intensive, hindering research into the biosynthesis and regulatory mechanisms of its valuable natural products. In recent years, the clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9) system has emerged as a powerful genome editing tool, extensively utilized in life sciences research due to its high efficiency and user-friendliness. This review provides a structured overview of advancements in CRISPR/Cas9-mediated genome editing technology in G. lucidum. We first discuss the development and optimization of the CRISPR system, focusing on the various expression strategies for Cas9 and guide RNA established in G. lucidum. Furthermore, we highlight the application of this system for targeted gene deletion, insertion, and replacement in genome editing, as well as its use in the functional analysis of genes in G. lucidum. In addition, we discuss the limitations and challenges associated with employing CRISPR/Cas9 tools in G. lucidum and provide an outlook on the future development of the CRISPR/Cas9 system and its applications in this organism.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"223"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial populations under fluoride stress: a metagenomic exploration from Indian soil.","authors":"Krishnendu Pramanik, Arup Sen, Subrata Dutta, Gouranga Sundar Mandal, Bappa Paramanik, Arpita Das, Nitin Chatterjee, Ankit Kumar Ghorai, Md Nasim Ali","doi":"10.1007/s11274-025-04408-5","DOIUrl":"https://doi.org/10.1007/s11274-025-04408-5","url":null,"abstract":"<p><p>Fluoride exposure, even at a low concentration, significantly impairs crop growth and productivity by inhibiting metabolic enzymes and disrupting photosynthesis. Addressing this challenge, microbial de-fluoridation emerges as a vital strategy to improve soil health, enhance crop growth, and ensure agricultural sustainability. This study analyzed topsoil samples (0-0.2 m depth) from rice fields in three blocks of Purulia district, West Bengal-Arsha, Jhalda-I, and Joypur. Fluoride content in the samples ranged from 58.76 ± 0.76 mg/kg to 282.9 ± 4.9 mg/kg (total) and 1.57 ± 0.02 mg/kg to 2.97 ± 0.03 mg/kg (available). The metagenomic analysis of the collected soil samples revealed diverse microbial communities comprising archaea, bacteria, fungi, and viruses, with Actinobacteria (phylum), Hyphomicrobiales (order), and Nocardioidaceae (family) being the dominant prokaryotes. Arsha soil with comparatively low fluoride contamination exhibited the highest microbial diversity (11,891 taxa), followed by Joypur (11,528 taxa) and Jhalda-I (11,358 taxa), with Arsha showing nearly double the unique microbial taxa compared to the other locations. Clusters of orthologous groups of proteins functional analysis identified 60,898 genes in Arsha, 63,403 genes in Jhalda-I, and 73,334 genes in Joypur, while Kyoto encyclopedia of genes and genomes analysis revealed 9,385, 9,104, and 10,633 genes, respectively. Key genes associated with fluoride metabolism-inorganic pyrophosphatase, divalent metal cation transporter mntH, and putative fluoride ion transporter crcB-were abundant across all sites, highlighting the influence of fluoride on microbial community structure. This study provides the first comprehensive report on soil microbial communities in fluoride-rich areas, highlighting the potential of native fluoride-tolerant microbes to mitigate fluoride toxicity in agricultural soils and offer sustainable, microbe-based solutions to fluoride contamination.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"221"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carvacrol induces apoptosis in Aspergillus niger through ROS burst.","authors":"Qingchao Gao, Zhiruo Feng, Zuli Wang, Fangyuan Zhao, Jian Ju","doi":"10.1007/s11274-025-04437-0","DOIUrl":"https://doi.org/10.1007/s11274-025-04437-0","url":null,"abstract":"<p><p>Carvacrol, a phenolic monoterpenoid and major active constituent of plant essential oils, exhibits broad-spectrum antimicrobial activity with potential applications in food preservation and agricultural biocontrol. Previous studies have shown that one of the antifungal mechanisms of carvacrol may be the induction of reactive oxygen species (ROS) burst, but its specific action mechanism is still unclear. This study investigated the apoptosis mechanism of Aspergillus niger caused by ROS burst induced by carvacrol in the molecular level. Carvacrol induced ROS burst, leading to the accumulation of intracellular hydrogen peroxide (H₂O₂) concentration in A. niger which caused the cellular oxidative stress and lipid peroxidation, leading to an increase in the content of unsaturated fatty acids as well as a decrease in the content of glycerophospholipids in the cell membrane, and then disrupted the integrity of cell membranes. RT-qPCR was used to analyze the expression levels of different subunit genes of NADPH oxidase, and the result showed that NoxA, Cdc24, NoxD and NoxR were all upregulated to varying degrees. And the inhibitory effect of carvacrol on A. niger can be alleviated by adding an additional ROS scavenger. Meanwhile, NADPH oxidase inhibitors eliminated the ROS production induced by carvacrol. In summary, carvacrol can induce ROS burst in A. niger by regulating the gene expression levels of different subunits of NADPH oxidase, leading to membrane lipid peroxidation, irreversible damage to the cell membrane, the disruption of redox dynamic balance and finally cell death.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"225"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gut microbiota-bile acid axis: a crucial regulator of immune function and metabolic health.","authors":"Anuradha Tyagi, Vinay Kumar","doi":"10.1007/s11274-025-04395-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04395-7","url":null,"abstract":"<p><p>The gut microbiota and bile acid metabolism are intricately linked, playing a crucial role in immune regulation, metabolic processes, and overall health. The gut microbiome, consisting of diverse bacterial genera such as Bacteroides, Clostridium, Lactobacillus, Bifidobacterium, and Eubacterium, facilitates the conversion of primary bile acids into secondary bile acids through enzymatic modifications. Bile acids, synthesized in the liver and modified by gut microbiota, act as signaling molecules that regulate immune responses via bile acid receptors, including the farnesoid X receptor (FXR), G protein-coupled bile acid receptor 1 (GPBAR1), pregnane X receptor (PXR), vitamin D receptor (VDR), and sphingosine-1-phosphate receptor 2 (S1PR2). Dysbiosis-an imbalance in gut microbial composition-disrupts bile acid metabolism, leading to impaired activation of bile acid receptors and contributing to various diseases. These include inflammatory bowel disease, metabolic disorders such as obesity and type 2 diabetes, autoimmune diseases like multiple sclerosis, and liver conditions such as cholestasis and non-alcoholic fatty liver disease. Dysfunctional bile acid receptor signaling further promotes chronic inflammation, metabolic dysregulation, and disturbances in gut-liver-immune homeostasis. Emerging therapeutic strategies targeting bile acid receptors, restoring microbiota balance, and implementing dietary interventions offer promising avenues for disease prevention and management. This review explores the pivotal role of gut microbiota in modulating immune responses through bile acid receptors and highlights their therapeutic potential in improving treatment outcomes.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"215"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Karakum desert: a unique source of cultivable novel and rare actinomycetes with a remarkable biosynthetic potential.","authors":"Hayrettin Saygin, Nevzat Sahin, Michael Goodfellow","doi":"10.1007/s11274-025-04399-3","DOIUrl":"10.1007/s11274-025-04399-3","url":null,"abstract":"<p><p>A culture-based strategy was used to isolate and screen representative actinomycetes from six sampling sites in the Karakum Desert, Turkmenistan. A total of 459 actinomycete isolates were obtained using 16 selective media, and 270 representative strains were subjected to 16 S rRNA gene sequencing. Comparative 16 S rRNA gene sequence analyses on colour-group representatives led to their assignment to 17 genera with validly published names which included many isolates assigned to novel or putatively novel species including ones belonging to rare genera, such as Actinocorallia, Actinomadura, Jiangella and Nonomuraea. Mining of whole-genome sequences of 32 isolates which formed distinct lineages in phylogenomic trees revealed biosynthetic gene clusters predicted to encode for many novel specialized metabolites, notably antibiotics. The genomes of most of these isolates included genes associated with the promotion of plant growth while bioinformatic analyses of stress-related genes provided on insight into how filamentous actinomycetes have adapted to harsh environmental conditions in the Karakum Desert. This extensive bioprospecting campaign shows that the Karakum Desert is a unique source of novel, rare and gifted filamentous actinomycetes with the ability to synthesise new specialized metabolites needed to address key existential issues facing humankind, especially, the urgent need to find a new generation of therapeutic antibiotics to control multidrug-resistant microbial pathogens and compounds that protect and promote plant growth.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"202"},"PeriodicalIF":4.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of electrolyzed oxidized water (EO) and peroxyacetic acid on plasmid-mediated quinolone resistance genes of Pseudomonas aeruginosa.","authors":"Hanan R Ghanayem, Khalid Tolba, Mariam M G El-Shemy, Fawzia A El-Shenawy","doi":"10.1007/s11274-025-04384-w","DOIUrl":"10.1007/s11274-025-04384-w","url":null,"abstract":"<p><p>Pseudomonas aeruginosa displays major biofilm formation, food spoilage, and significant losses for the food industry. It extremely exhibits inherent resistance to various antibiotics, including quinolones, which can spread via plasmids. The present study investigates the prevalence of biofilm-forming P. aeruginosa in meat products and assesses its antibiotic resistance patterns. Evaluating the effectiveness of electrolyzed-oxidized water (EOW) and per-acetic acid (PAA) alone or in a combination against P. aeruginosa biofilm formed on stainless steel (SS) surfaces. Besides, the effect on quinolone-resistance (qnr) gene expression level using real-time PCR. P. aeruginosa was isolated from 24.6% of the total tested samples (37/150), with a significant variation observed regarding the contamination levels of the tested products. 36.3% of the isolates demonstrated robust biofilm production, with 82.3% displaying multi-drug resistance (MDR). Isolates revealed complete susceptibility to amikacin (100%) and gentamycin (82%). Quinolone resistance was observed in 23% and 17% of the isolates for ciprofloxacin and levofloxacin, respectively. 80% (4/5) among the confirmed isolates were enclosed the plasmid qnr genes. The genes pslA and gyrA were detected in 40% and 60%, respectively. EOW, particularly when combined with PAA, reveals strong antibacterial activity against P. aeruginosa and greatly decreases its count. Moreover, it can eliminate its biofilm within 20 min of exposure and significantly decrease the expression levels of quinolone-resistant genes. In conclusion, PAA and EOW are effective agents for biofilm control on SS surfaces, particularly when used in combined form, and could be more effective in combating resistant bacterial infections and controlling the spread of PMQR.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"199"},"PeriodicalIF":4.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial bacteria-based bioformulations as potential biocontrol and biocleaning solutions for stone heritage conservation.","authors":"Tamara Janakiev, Ivica Dimkić, Jovana Aleksić, Milica Ljaljević Grbić, Aleksandar Knežević, Janez Kosel, Črtomir Tavzes, Nikola Unković","doi":"10.1007/s11274-025-04446-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04446-z","url":null,"abstract":"<p><p>Indigenous bacterial community of the deteriorated Rožanec Mithraeum monument (Slovenia) was analyzed as a basis for development of a synergistic bacterial consortium with potential to be re-introduced for biocontrol and biocleaning treatment of infested limestone monument. Core community constituents, based on the 16S rRNA gene sequences metabarcoding analysis, were representatives of Proteobacteria, Actinobacteriota, and Cyanobacteria phyla. Bacteria of Bacillus and Paenibacillus genera dominated in the culturable bacterial community. The most extensive enzymatic potential, as a foundation of biocleaning process, was observed for Bacillus mycoides MIT8.7 and Paenibacillus amylolyticus/taichungensis/tylopili/tundrae MIT8.18. In general, representatives of Bacillus genus demonstrated excellent amylase and protease potential, with small lipase, cellulase, mannanase, and xylanase activity. The highest PGI% values, against 7 autochthonous biodeteriogenic fungi, were measured for Bacillus velezensis MIT7.8 and Pseudomonas chlororaphis subsp. aurantiaca MIT4.11 highlighting them as the most promising bacterial antagonist for the consortium. Compared to commercial biocides 0.3% Preventol RI80, 100% Keim, and 100% BFA, recommended for application in stone conservation, Streptomyces anulatus 1-3 TSA and Streptomyces sp. 11-11MM full cultures also proved efficient and lacked any corrosive effect as reflected in unaltered surface roughness parameters Rq and Ra, over the 3 months application period on laboratory models made of limestone obtained in the proximity of the Rožanec Mithraeum relief. Characterized bacterial candidates possess potential to be used for development of a bacterial consortium that can be utilized in situ as a natural, environment friendly, and safe alternative effective in the conservation of stone heritage via joint activity making an important step towards desired widespread exclusion of biocide application.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"200"},"PeriodicalIF":4.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice endophytic fungal community and its potential role on rice grains Cd accumulation.","authors":"Weijun Gong, Qiaohong Li, Yibin Lai, Dian Yang, Xiaogang Xu, Haishan Liao, Hongen Wang, Fenglian Cai, Haiyan Li","doi":"10.1007/s11274-025-04449-w","DOIUrl":"https://doi.org/10.1007/s11274-025-04449-w","url":null,"abstract":"<p><p>Rice is a cadmium (Cd) high-accumulator, therefore, the soils Cd contamination may cause food security problems. Endophytes played a crucial role on host plants' heavy metal accumulation. However, the endophytic fungal community of rice and its role on rice grains Cd accumulation is still unclear. In this study, the endophytic fungal community of rice at different growth stages from different Cd-contaminated sites (LC and HC, contains lower and higher concentration of Cd, respectively) were investigated by high-throughput sequencing method. In addition, the culturable fungal endophytes were isolated and Cd tolerance assessments were also conducted for some of the isolates. The results showed that the soils Cd concentration and rice tissue showed greater effect on the endophytic fungal community than that of the rice growth stage. The dominant endophytic fungi changed with the soils Cd concentration, and higher Cd concentration reduced the species diversity (Shannon index) and evenness (Evenness index) of endophytic fungi, especially in the roots. In addition, the correlation analysis of dominant endophytic fungi with environmental factors showed that Alternaria, Fusarium and Saccharomyces had a significant positive correlation with Cd concentration. While, the linear discriminant analysis effect size (LEfSe) analysis showed that Stellatospora, Westerdykella, Sarocladium, Spencerozyma and Penicillium were the biomarkers (the relative abundance significantly increased) in multiple tissues from higher Cd-contaminated site. The endophytic fungi from site HC possessed higher ration of Cd tolerant isolates, and the tolerant isolates belong to Sarocladium, Alternaria, Fusarium, etc. Notably, the co-occurrence networks showed that higher Cd concentration increased modularity and number of communities of rice endophytes, especially in the roots, while decreased the positive correlation among different endophytic groups (genus level) in different tissues. These results suggested that rice may respond to higher Cd stress through enriching Cd tolerant endophytic fungi. While, the endophytic fungal community affected rice Cd tolerance by improving its microbiome stability, diversifying microbial survival strategies and maintaining the ecological balance.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"201"},"PeriodicalIF":4.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of lipase and extracellular polymeric substances by the lipid-degrading bacterium Burkholderia arboris strain JYK2 in response to different substrates.","authors":"Jiyu Huang, Mei-Fang Chien, Hernando P Bacosa, Chihiro Inoue","doi":"10.1007/s11274-025-04432-5","DOIUrl":"10.1007/s11274-025-04432-5","url":null,"abstract":"<p><p>Characterization of lipid-degrading microorganisms and understanding their metabolite production mechanisms are essential for enhancing biodegradation efficiency of lipid-rich wastewater. In this study, we isolated Burkholderia arboris strain JYK2, which demonstrated lipid degradation at 46.29 mg/(l • h) with lipase activity reaching 59.92 U/ml when lipid was provided as the sole carbon source. Experimental results revealed that strain JYK2 secretes lipase in media containing lipids and fatty acids but not in glycerol-containing media, a phenomenon likely attributable to quorum sensing mechanisms. Furthermore, extracellular polymeric substances (EPS) were produced in the presence of lipids and fatty acids. Compositional analysis showed that EPS primarily consisted of proteins (approximately 50%) and polysaccharides (approximately 25%). This protein-rich characteristic conferred high hydrophobicity to the EPS, contributing to its lipase adsorption capacity as verified in this study. Additionally, biosurfactant activity was detected in the EPS produced by JYK2. These functions collectively enhance substrate utilization by microorganisms and promote lipid biodegradation. However, minimal EPS production was observed at low fatty acid concentrations, suggesting that the EPS production mechanism cannot be solely attributed to quorum sensing. This study provides insights into the interactions between lipid-degrading bacteria, lipase production, and EPS functionality, which are crucial for optimizing biological treatment of lipid-rich wastewater.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"198"},"PeriodicalIF":4.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Control of aflatoxin in broiler feeds through dietary supplementation with Pichia kudriavzevii B-5P: effects on performance, serum biochemistry and histopathological change - a promosing strategy.","authors":"Lili Anggraini, Yetti Marlida, Harnentis, Yuliaty Shafan Nur, Laily Rinda Ardani","doi":"10.1007/s11274-025-04436-1","DOIUrl":"https://doi.org/10.1007/s11274-025-04436-1","url":null,"abstract":"<p><p>Aflatoxin is a mycotoxin that contaminates many grain feed ingredients, especially corn. Pichia kudriavzevii B-5P is a yeast strain with potential application in the mitigation of Aspergillus flavus and its associated aflatoxins. This research aims to optimize the dose of Pichia kudriavzevii B-5P as a toxin binder in corn containing aflatoxin in broiler chicken rations along with in vivo evaluation of its protection efficiency on performance, carcass characteristics, serum biochemistry and histopatholical alteration in the liver, kidneys and intestines. This research used a Completely Randomized Design (CRD), 4 treatments and 5 replications. Treatment consisted of T1: control (no aflatoxin); T2: 0,1% P. kudriavzevii B-5P; T3: 0,2% P. kudriavzevii B-5P; T4: 0,3% P. kudriavzevii B-5P. Performance (consumption, weight gain, feed conversion, live weight, and carcass), abdominal fat, percentage of organ weight (liver, kidney, and small intestine), and total protein, albumin, and globulin were not significantly different (p > 0.05) between the control group (without aflatoxin) and the group of chickens fed aflatoxin (191.95 ppb) + Pichia kudriavzevii B-5P. Histopathologically, the liver, kidneys, and small intestine showed mild damage that tended to improve with the administration of P. kudriavzevii B-5P, thereby not affecting organ function. The results of the study concluded that giving Pichia kudriavzevii B-5P as much as 0.1% in feed can overcome aflatoxin contamination with a level of 191.95 ppb in broiler chickens.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"195"},"PeriodicalIF":4.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}