World journal of microbiology & biotechnology最新文献

{"title":"Systematic review and meta-analysis of the potential effects of endophytic bacteria Klebsiella on plant growth promotion and biocontrol of pathogens.","authors":"Odyone Nascimento da Silva Bandeira, Renato da Silva Bandeira, Cláudia Regina Batista de Souza","doi":"10.1007/s11274-025-04300-2","DOIUrl":"10.1007/s11274-025-04300-2","url":null,"abstract":"<p><p>Klebsiella is a bacterial genus widely recognized in the medical field but with underexplored potential in agriculture. This study employed a systematic review and meta-analysis to investigate scientific articles on plant growth promotion effects associated with endophytic bacteria Klebsiella species. A total of 39 relevant studies, published between 2012 and 2024, were identified based on strict inclusion and exclusion criteria. The analysis revealed that Klebsiella sp., K. pneumoniae, and K. variicola are cosmopolitan species that have functional versatility in phytohormone production, nutrient solubilization, and pathogen control in agricultural systems in both tropical and temperate zones. The data showed a significant correlation between the use of Klebsiella sp. and plant growth, highlighting the positive impact of these species in controlling aggressive pathogens. These findings underscore the potential of Klebsiella as a biotechnological tool for sustainable agricultural practices, enhancing plant growth and reducing the reliance on chemical inputs. The study further emphasizes the need for future research to deepen genomic characterization and expand the agricultural applications of these bacteria.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"89"},"PeriodicalIF":4.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting disease resistance in Solanum melongena L. (eggplant) against Alternaria solani: the synergistic effect of biocontrol Acinetobacter sp. and indole-3-acetic acid (IAA).","authors":"Mohammad Danish, Mohammad Shahid, Zaryab Shafi, Mohammad Tarique Zeyad, Mohammad Abul Farah, Khalid Mashay Al-Anazi, Lukman Ahamad","doi":"10.1007/s11274-025-04282-1","DOIUrl":"10.1007/s11274-025-04282-1","url":null,"abstract":"<p><p>Alternaria solani causes early blight disease in eggplants, threatening production and leading to significant economic losses. Fungicides are used to control fungal diseases, but their overuse raises resistance concerns. Finding novel, eco-friendly biocontrol agents is therefore a solution for the future. The coordination between antagonistic bacterial agents and plant growth hormones in defense responses against fungal pathogens are crucial. This study assessed biocontrol potential of Acinetobacter sp. SCR-11 (Accession no. OR751536.1) and indole-3-acetic acid (IAA; 100 µM), singly and in combination, against A. solani in eggplants. Strain SCR-11 produced hydrogen cyanide (HCN; 5.7 µg mL⁻¹), siderophore i.e. salicylic acid (14.7 µg mL⁻¹), 2,3-dihydroxybenzoic acid (23.1 µg mL⁻¹) and various extracellular lytic enzymes. Strain SCR-11 exhibited antagonistic activity by strongly inhibiting (82%) A. solani. Acinetobacter sp. inoculation and IAA treatment enhanced growth, biomass, and leaf pigments of A. solani-diseased eggplants, with effectiveness in order: SCR-11 + IAA > SCR-11 > IAA >. The combined treatments (SCR-11 + IAA) most effectively increased total soluble protein (62.5%), carbohydrate (60%), total soluble sugar (81%), and phenol (74%) in A. solani-infected eggplant. Biocontrol agent and IAA application significantly (p ≤ 0.05) reduced proline and malondialdehyde (MDA) levels, alleviating oxidative stress in A. solani-diseased eggplant. The SCR-11 + IAA treatment significantly reduced the percent disease index (71%) and increased protection (69%) in diseased eggplant. The Acinetobacter sp. and IAA coordination enhanced disease resistance in A. solani-infected eggplants by boosting defense enzyme activities (SOD, POD, PAL, and β-1, 3 glucanase), significantly protecting plants from pathogen attack. At harvest, soil populations of A. solani decreased, while SCR-11 populations increased significantly. Acinetobacter sp. and IAA work synergistically through pathogen suppression, plant growth promotion, and induction of plant defense responses. Thus, applying antagonistic PGPR strain with exogenous IAA enhances eggplant resistance to A. solani, providing an environmentally friendly agricultural solution.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"85"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable microbial solutions for managing fungal threats in wheat: progress and future directions.","authors":"Prem Lal Kashyap, Sudheer Kumar, Annie Khanna, Poonam Jasrotia, Gyanendra Singh","doi":"10.1007/s11274-025-04286-x","DOIUrl":"10.1007/s11274-025-04286-x","url":null,"abstract":"<p><p>Biotrophic and necrotrophic fungi are responsible for causing a range of diseases in wheat, resulting in significant economic losses and a decline in quality. Effective management of these diseases generally involves a combination of resistance breeding, chemical treatments, and cultural practices. However, traditional breeding methods have made limited progress due to the slow pace of genetic improvements, the complexity of the wheat genome, and the quantitative nature of disease resistance traits, along with the constantly evolving virulence of pathogens. This situation has prompted research into more effective and eco-friendly alternatives, such as biological control. Recent studies have concentrated on using antagonistic microbes to decrease the reliance on chemical pesticides while enhancing wheat health and productivity. A comprehensive overview of current knowledge on wheat disease outbreaks is being developed, with a focus on advancements in biological control strategies. The review will first discuss the key fungal pathogens and their associated diseases, followed by a summary of biological control methods, particularly emphasizing potential microbial antagonists. Additionally, it will explore strategies to improve the efficacy of biocontrol agents, which are crucial for a holistic and sustainable approach to wheat disease management. Ultimately, the article will highlight the role of biological control in promoting more sustainable agricultural practices, particularly concerning wheat diseases, in alignment with the UN sustainable development goals.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"79"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Underlying the early signaling and gene expression of Pleurotus ostreatus mycelia during injury response.","authors":"Jiachun Zhu, Jiaqi Sun, Huiping Li, Ping Xu, Lin Ma, Qiang Wang, Keping Chen, Shaoxuan Qu","doi":"10.1007/s11274-025-04296-9","DOIUrl":"10.1007/s11274-025-04296-9","url":null,"abstract":"<p><p>Plants and animals have demonstrated conserved damage defense systems, but the injury response mechanisms in fungi remain largely unexplored. In this study, we investigate the early defensive mechanisms activated by mechanical damage in the basidiomycota Pleurotus ostreatus. Assays of defense enzymes and gene expression in P. ostreatus revealed the role of reactive oxygen species (ROS) and the activation of the mitogen-activated protein kinase (MAPK) signaling pathway in this process. The findings from the defense-related enzyme tests showed that damage enhanced NAD/NADPH-dependent ROS production and endogenous carbon monoxide expression. Additionally, qRT-PCR data provided further evidence for H₂O₂ generation, highlighting the involvement of MAPK signaling and several early defense components in the damage response of P. ostreatus. Notably, there were differences in the damage response among various pest-resistant strains, with several genes specifically expressed in the pest-resistant strain. Pretreatment of damaged mycelium with N, N'-dimethylthiourea (DMTU, a hydroxyl radical scavenger), diphenyleneiodonium chloride (DPI, a NADPH oxidase inhibitor), or BAPTA (a calcium chelator) significantly inhibited hyphal regeneration and growth. These findings also imply that Ca²⁺ was an another important signaling molecule in response to wounding. This study provides a more comprehensive holistic view on the damage response of P. ostreatus, and offers valuable references for future research into its biological traits and prospective uses.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"80"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in the cyanobacterial products and applications of phycocyanins.","authors":"Jinichi Aoki, Runa Koshikawa, Munehiko Asayama","doi":"10.1007/s11274-025-04297-8","DOIUrl":"10.1007/s11274-025-04297-8","url":null,"abstract":"<p><p>Recent developments in the research on cyanobacterial products have drawn increasing attention, especially in the production and application of phycocyanin, which has shown great potential in various fields. Cyanobacteria are photosynthetic prokaryotes that live on Earth and are the ancestors of plant chloroplasts. They have a compact genome size compared to other eukaryotic photosynthesizing microorganisms; some species are genetically engineered and have high growth potential in indoor culture, and some strainscan maintain high growth potential even in outdoor culture. Cyanobacteria are valuable because they can selectively and effectively produce and recover useful substances that are poorly produced by other microalgae, although this depends on the algal species. However, the social implementation of biorefineries using cyanobacteria involves issues such as setting up useful products in addition to the culture methods and strains to be used. This review aims to present research trends over the last 20 years on the production of useful substances such as biodegradable plastics, lipids, polysaccharides, and pigment proteins (phycocyanins) from cyanobacteria. Phycocyanin is mainly recovered and purified by filamentous cyanobacteria and has contributed to the research field, especially in the food and beverage industry. Additionally, the production and functions of phycocyanin are summarized to provide a better understanding of these possibilities. Their potential applications as environmentally friendly materials are also described to further contribute to the research field and social implementation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"84"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in fluorescent labeling in assessing the probiotic adhesion capacity - A review.","authors":"Weiping Xu, Han Tan, Peiling Hu, Shijian Liu, Jiajia Song, Xiaoyong Chen, Huayi Suo","doi":"10.1007/s11274-024-04186-6","DOIUrl":"10.1007/s11274-024-04186-6","url":null,"abstract":"<p><p>Adhesion capacity of probiotics is closely related to their intestinal-protective effects. The conventional techniques used to evaluate probiotic adhesion capacity have limitations in terms of imaging resolution and quantitative analysis. Fluorescent labelling technology has shown immense potential in recent years owing to its high specificity and sensitivity for resolving probiotic adhesion mechanisms. Although there are still problems with the fluorescence signal intensity and hysteresis effect, this technology has significantly advanced the accurate detection and evaluation of probiotic adhesion capacity. This review examines the critical role of probiotic adhesion and its detection methods, with a special focus on the application of fluorescent-labeling technology. Our objective was to identify more accurate and efficient approaches for evaluating the adhesion capacity of probiotic bacteria while promoting in-depth research into the underlying mechanisms that govern probiotic adhesion.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"73"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amphibian skin bacteria contain a wide repertoire of genes linked to their antifungal capacities.","authors":"Francisco González-Serrano, Yordan J Romero-Contreras, Alberto H Orta, M Delia Basanta, Hugo Morales, Gabriela Sandoval García, Elena Bello-López, A S Escobedo-Muñoz, Víctor H Bustamante, Víctor Ávila-Akerberg, Miguel Ángel Cevallos, Mario Serrano, Eria A Rebollar","doi":"10.1007/s11274-025-04292-z","DOIUrl":"10.1007/s11274-025-04292-z","url":null,"abstract":"<p><p>Emerging diseases caused by fungi are a serious threat to wildlife biodiversity. The widespread fungal pathogen Batrachochytrium dendrobatidis (Bd) has caused dramatic amphibian population declines and species extinctions worldwide. While many amphibians have been negatively affected by Bd, some populations/species have persisted despite its presence. One factor contributing to amphibian protection against this fungus is the host-associated skin microbiome. In this study, we aimed to identify gene clusters associated with the antifungal activity of amphibian skin bacteria. Specifically, we explored skin bacteria isolated from species that have persisted in the wild despite the presence of Bd: the frogs Agalychnis callidryas, Craugastor fitzingeri, Dendropsophus ebraccatus, and the axolotl Ambystoma altamirani. Bacterial isolates were tested in vitro for their capacity to inhibit the growth of two fungal pathogens: Bd and the phytopathogen Botrytis cinerea (Bc). Genome mining of these bacterial isolates revealed a diverse repertoire of Biosynthetic Gene Clusters (BGCs) and chitin-degrading gene families (ChDGFs) whose composition and abundance differed among bacterial families. We found specific BGCs and ChDGFs that were associated with the capacity of bacteria to inhibit the growth of either Bd or Bc, suggesting that distinct fungi could be inhibited by different molecular mechanisms. By using similarity networks and machine learning, we identified BGCs encoding known antifungal compounds such as viscosin, fengycin, zwittermicin, as well as siderophores and a novel family of beta-lactones. Finally, we propose that the diversity of BGCs found in amphibian skin bacteria comprise a substantial genetic reservoir that could collectively explain the antifungal properties of the amphibian skin microbiome.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"78"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remodeling of bacterial nitrilase active pocket improves the capability to degrade glucosinolate-derived nitriles.","authors":"Ming-Yu Liu, Yu-Yue Wang, Han-Zhi Li, Xue-Mei Luo, Jia-Xun Feng, Shuai Zhao","doi":"10.1007/s11274-025-04294-x","DOIUrl":"10.1007/s11274-025-04294-x","url":null,"abstract":"<p><p>Nitriles exhibit acute cytotoxicity to human and animal cells. Nitrilase is a green biocatalyst that can directly convert nitrile into nontoxic carboxylic acids and ammonia. However, the nitrilases capable of degrading 3-butenenitrile and 4-pentenenitrile derived from glucosinolate present in rapeseed meals are still limited. This study expressed the nitrilase 2 gene from bacterium Paraburkholderia graminis (PgNIT2) in Escherichia coli. The purified recombinant nitrilase rPgNIT2 showed specific activities of 5.94 U/mg and 6.66 U/mg when using 3-butenenitrile and 4-pentenenitrile as substrates at the optimal pH 7.0 and temperature 45 °C. The substrate binding pocket of PgNIT2 comprising 12 amino acids was required for rPgNIT2 activity. Remarkably, the specific activity of mutant enzyme A190I increased by 68.3% and 57.3%, respectively, against 3-butenenitrile and 4-pentenenitrile compared with the wild-type enzyme. Furthermore, the A190I showed improved thermostability and hydrolysis ability towards 3-butenenitrile and 4-pentenenitrile compared with rPgNIT2 but shared the same optimal pH and temperature. These results provided theoretical guidance for engineering of bacterial nitrilases used for rapeseed meal detoxification.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"82"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The multifaceted roles of gut microbiota in insect physiology, metabolism, and environmental adaptation: implications for pest management strategies.","authors":"Kamran Haider, Dilawar Abbas, Jose Galian, Muhammad Adeel Ghafar, Kamil Kabir, Muhammad Ijaz, Mehboob Hussain, Khalid Ali Khan, Hamed A Ghramh, Abbas Raza","doi":"10.1007/s11274-025-04288-9","DOIUrl":"10.1007/s11274-025-04288-9","url":null,"abstract":"<p><p>Similar to many other organisms, insects like Drosophila melanogaster, Hypothenemus hampei, and Cockroaches harbor diverse bacterial communities in their gastrointestinal systems. These bacteria, along with other microorganisms like fungi and archaea, are essential to the physiology of their insect hosts, forming intricate symbiotic relationships. These gut-associated microorganisms contribute to various vital functions, including digestion, nutrient absorption, immune regulation, and behavioral modulation. Notably, gut microbiota facilitates the breakdown of complex plant materials, synthesizes essential vitamins and amino acids, and detoxifies harmful substances, including pesticides. Furthermore, these microorganisms are integral to modulating host immune responses and enhancing disease resistance. This review examines the multifaceted roles of gut microbiota in insect physiology, with particular emphasis on their contributions to digestion, detoxification, reproduction, and environmental adaptability. The potential applications of gut microbiota in integrated pest management (IPM) are also explored. Understanding the microbial dynamics within insect pest species opens new avenues for pest control, including developing microbial biocontrol agents, microbial modifications to reduce pesticide resistance, and implementing microbiome-based genetic strategies. In particular, manipulating gut microbiota presents a promising approach to pest management, offering a sustainable and eco-friendly alternative to conventional chemical pesticides.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"75"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylic acid-induced upregulation of AtAACT and AtMVD expression enhances triterpene biosynthesis in Athelia termitophila.","authors":"Fangcheng Hu, Yonggang Fang, Lianxi Xing","doi":"10.1007/s11274-025-04301-1","DOIUrl":"10.1007/s11274-025-04301-1","url":null,"abstract":"<p><p>Triterpenoids exhibit great potential in the food and pharmaceutical industries and are the predominant secondary metabolites of Athelia termitophila (TMB). AtAACT catalyzes the conversion of acetyl-CoA and acetoacetate in mevalonate biosynthesis, while AtMVD catalyzes the decarboxylation of mevalonate diphosphate, producing key precursors essential for triterpenoid synthesis. To augment the biosynthesis of TMB triterpenes, we cloned the AtAACT and AtMVD genes into plasmids, which were subsequently used to transform Escherichia coli. The resulting bacterial strains were used for sequencing and bioinformatic analyses to elucidate the encoded amino acid sequences. Furthermore, salicylic acid (SA) was employed as an elicitor to enhance triterpene biosynthesis in TMB. The SA treatment was initiated on the 6th day of incubation and maintained continuously across all time points (36, 48, and 60 h and others), achieving a maximal triterpene concentration of 41.83 ± 0.5 mg/100 mL, corresponding to a 26% increase compared to the uninduced group. Comparative transcriptomic analysis showed that the expression of AtAACT and AtMVD was significantly upregulated in the SA-treated group compared to the control. This upregulation underscores the crucial roles of these genes in facilitating triterpene biosynthesis in TMB. Furthermore, qPCR temporal profiling revealed that AtAACT achieved peak transcript levels at 36 h post-induction, whereas AtMVD peaked at 48 h. This study provides an effective strategy to enhance TMB triterpene content and offers new insights into the mechanisms of SA-treated triterpene biosynthesis.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 3","pages":"87"},"PeriodicalIF":4.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}