World journal of microbiology & biotechnology最新文献

{"title":"Cold-adapted characteristics and gene knockout of alkyl hydroperoxide reductase subunit C in Antarctic Psychrobacter sp. ANT206.","authors":"Yanhua Hou, Jiarui Qiao, Shumiao Hou, Yatong Wang, Quanfu Wang","doi":"10.1007/s11274-024-04158-w","DOIUrl":"10.1007/s11274-024-04158-w","url":null,"abstract":"<p><p>Alkyl hydroperoxide reductase subunit C (AhpC) contributes to the cellular defense against reactive oxygen species. However, it remains understudied in psychrophiles. Amino acid comparison demonstrated that AhpC from Psychrobacter sp. ANT206 (ANT206) (PsAhpC) revealed fewer numbers of Lys and more numbers of Gly, which might have favored higher flexibility at low temperature. The recombinant PsAhpC (rPsAhpC) was most active at 25 °C and retained 35% of its residual activity at 0 °C, indicating that it was a cold-adapted enzyme. Additionally, rPsAhpC demonstrated significant salt tolerance, sustaining its activity in the presence of 4.0 M NaCl. Molecular dynamics simulations indicated that PsAhpC had comparatively loose conformation, which facilitated reactions at low temperatures. Subsequently, an ahpc knockout mutant was constructed, and the growth rate of the knockout mutant significantly decreased, suggesting that ahpc might be crucial for the growth of ANT206 at low temperatures. The findings provide a robust foundation for further investigation into the structural features and catalytic characterization of cold-adapted AhpC. The structural characteristics of PsAhpC and its cold tolerance and salt tolerance may be applied to stress resistance breeding of various organisms.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"359"},"PeriodicalIF":4.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the eco-friendly herbicidal potential of microbial metabolites from Bacillus altitudinis.","authors":"Xiu-Hua Ma, Shuo Shen, Wei Li, Jian Wang","doi":"10.1007/s11274-024-04154-0","DOIUrl":"10.1007/s11274-024-04154-0","url":null,"abstract":"<p><p>Microbial herbicides play a vital role in agricultural preservation, amid growing concerns over the ecological impact from extensive development and use of chemical herbicides. Utilizing beneficial microbial metabolites to combat weeds has become a significant focus of research. This study focused on isolating herbicidal active compounds from Bacillus altitudinis D30202 through activity-guided methods. First, the n-butanol extract (n-BE) of B. altitudinis D30202 underwent fractionation using macroporous adsorption resin D101 and Sephadex LH-20, identifying Fr. F as the most potent segment against wild oats (Avena fatua L.). Ultra-performance liquid chromatography - quadrupole time-of-flight mass spectrometry (UPLC - QTOF-MS) identified nine compounds in the active fraction Fr. F. Subsequently, three subfractions (Fr.F-1 to Fr.F-3) were derived from Fr.F via semi-preparative liquid chromatography, resulting in methyl indole-3-acetate (MeIAA) purification. MeIAA, functioning as an auxin analog, exhibited effects of indole-3-acetic acid (IAA) on wild oats' growth, with a root length median inhibitory concentration of 81.06 µg/ml. Furthermore, we assessed MeIAA's herbicidal impact on five weed species across diverse families and genera, providing a first-time analysis of MeIAA's mechanism on wild oats. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed structural damage to leaves and roots post-MeIAA treatment. MeIAA treatment increased superoxide anion and hydrogen peroxide levels in wild oat roots, alongside with elevated peroxidase (POD) and superoxide dismutase (SOD) activity, chlorophyll-degrading enzymes (Chlase, MDACase), malondialdehyde (MDA) content, and relative conductivity in leaves. Conversely, it decreased catalase (CAT) activity and chlorophyll content. Therefore, this study provides a new material source and theoretical foundation for ecologically sustainable agricultural weed control.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"356"},"PeriodicalIF":4.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights and functional analysis of isocitrate dehydrogenase in two gram-negative pathogenic bacteria.","authors":"Wei Xiong, Rui Su, Xueyang Han, Mengxiao Zhu, Hongyiru Tang, Shiping Huang, Peng Wang, Guoping Zhu","doi":"10.1007/s11274-024-04169-7","DOIUrl":"10.1007/s11274-024-04169-7","url":null,"abstract":"<p><p>Klebsiella pneumoniae and Legionella pneumophila are common Gram-negative bacteria that can cause lung infections. The multidrug resistance of K. pneumoniae presents a significant challenge for treatment. This study focuses on isocitrate dehydrogenase (IDH), a key enzyme in the oxidative metabolic pathway of these two bacteria. KpIDH and LpIDH were successfully overexpressed and purified, and their biochemical characteristics were thoroughly investigated. The study revealed that KpIDH and LpIDH are homodimeric enzymes with molecular weights of approximately 70 kDa. They are completely dependent on the coenzyme NADP⁺ and are inactive towards NAD⁺. KpIDH exhibits the highest catalytic activity at pH 8.0 in the presence of Mn²⁺ and at pH 7.8 in the presence of Mg²⁺. Its optimal catalytic performance is achieved with both ions at 55 °C. LpIDH exhibited its highest activity at pH 7.8 in the presence of Mn²⁺ and Mg²⁺, respectively, and exhibits optimal catalytic performance at 45 °C. Heat inactivation studies showed that KpIDH and LpIDH retained over 80% of their activity after being exposed to 45 °C for 20 min. Furthermore, we successfully altered the coenzyme specificity of KpIDH and LpIDH from NADP⁺ to NAD⁺ by replacing four key amino acid residues. This study provides a comprehensive biochemical characterization of two multidrug-resistant bacterial IDHs commonly found in hospital environments. It enhances our understanding of the characteristics of pathogenic bacteria and serves as a reference for developing new therapeutic strategies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"357"},"PeriodicalIF":4.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical and thermodynamic properties of purified rhodanese from A. welwitschiae LOT1 and the cyanide detoxification potential of the enzyme.","authors":"Olusola T Lawal, David M Sanni","doi":"10.1007/s11274-024-04164-y","DOIUrl":"10.1007/s11274-024-04164-y","url":null,"abstract":"<p><p>Rhodanese, the primary cyanide-detoxifying enzyme, plays a crucial role in mitigating the harmful effects of cyanide present in various industrial waste materials, such as battery manufacturing effluents. The bioremediation of cyanide-contaminated environments relies on efficient detoxification mechanisms, making rhodanese a valuable enzyme for biotechnological applications. This research aimed to investigate the biochemical properties of purified rhodanese produced by Aspergillus welwitschiae LOT1, a fungal strain with promising cyanide detoxification capabilities. The purified rhodanese was obtained through fermentation, precipitation, and chromatographic separations, resulting in a homogeneous band of approximately 58 kDa with a specific activity of 374 RU/mg, 28-fold purification, and 14% recovery. The enzyme exhibited optimal cyanide detoxification at pH 7 and 60 °C, with stability observed between 30 and 50 °C and pH 8-10. All metal ions examined except for Cu²⁺ enhanced the cyanide-degrading ability of rhodanese. Notably, the enzyme demonstrated a high substrate preference for Na₂S₂O₃ and followed a first-order kinetic model and free energy, ΔG of 61.3 kJ/mol, making it a promising candidate for biotechnological applications. Overall, this study provides valuable insights into the biochemical properties of rhodanese from A. welwitschiae LOT1, highlighting its potential for efficient cyanide detoxification and bioremediation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"355"},"PeriodicalIF":4.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus australimaris protect Gloriosa superba L. against Alternaria alternata infestation.","authors":"Pradeep Semwal, Shashank Kumar Mishra, Basudev Majhi, Abhilasha Mishra, Harshita Joshi, Sankalp Misra, Ankita Misra, Sharad Srivastava, Puneet Singh Chauhan","doi":"10.1007/s11274-024-04156-y","DOIUrl":"10.1007/s11274-024-04156-y","url":null,"abstract":"<p><p>Gloriosa superba L., a medicinally important plant, is often affected by leaf blight disease caused by Alternaria alternata, which compromises its productivity. This study explores the protective effects of Bacillus australimaris endophyte (NBRI GS34), demonstrating that its inoculation not only inhibits the disease but also promotes plant growth and increases the concentrations of bioactive metabolites. Co-culturing NBRI GS34 with A. alternata significantly boosts protease (30-50%) and chitinase (6-28%) activities, evidencing a synergistic interaction. Scanning electron microscopy and GC-MS analysis confirm NBRI GS34's antagonistic action and reveal antifungal compounds like undecanoic acid and benzene carboxylic acid in treatments. Greenhouse experiments show a 78% reduction in disease incidence with NBRI GS34 treatment, enhancing vegetative growth and upregulating defense-related genes. Additionally, HPLC analysis reveals increased gloriosine and colchicine concentrations by 52% and 33%, respectively. These findings suggest NBRI GS34 could serve as a sustainable fungicide alternative, enhancing the production of medically valuable compounds and highlighting its potential pharmaceutical applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"354"},"PeriodicalIF":4.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and microbial characterization of a forest litter-based bio-fertilizer produced in batch culture by fermentation under different initial oxygen concentrations.","authors":"Alejandra Gutierrez, Catherine Rébufa, Anne-Marie Farnet Da Silva, Sylvain Davidson, Lisa Foli, Yannick Combet-Blanc, Martine Martinez, Pierre Christen","doi":"10.1007/s11274-024-04155-z","DOIUrl":"10.1007/s11274-024-04155-z","url":null,"abstract":"<p><p>This work focused on the physico-chemical, biochemical and microbiological characterization of a new organic fertilizer based on fermented forest litter (FFL) mixed with agro-industrial by-products, on the culture realized in airtight glass bottle. Under strict anaerobiosis (0% initial oxygen concentration (IOC)), after a 16-day batch culture, the bottle-headspace analysis showed that the specific CO₂ production rate was low (0.014 mL/h.g dry matter) compared to those reached under aerobic conditions (e.g. 0.464 mL/h.g dm at 21% IOC). Moreover, the culture displayed a slight fermented fruity odour, mainly due to ethanol and ethyl acetate detected in the headspace (335 µL and 58.6 µL accumulated, respectively). The FFL organic matter degradation followed by infrared spectroscopy and catabolic potential and diversity characterized by BIOLOG^® EcoPlates were poor and pH dropped to 4.54. The microbiome's metabolism was oriented toward lactic fermentation with medium acidification, enrichment in lactic acid bacteria (LAB), depletion in fungi and absence of pathogens. By increasing IOC from 0 to 21%, the respirometric activity, and the catabolic potential and diversity increased. However, some enterobacteria were detected above 5% IOC. Ethanol and ethyl acetate decreased strongly with IOC, and aromatics and proteins contained in the solid matrix remained in the culture. This study showed the importance of oxygen on the final product. A 2% IOC was found to ensure an optimal balance between LAB development, preservation of functional catabolic diversity and bio-product free of microbial pathogens.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"353"},"PeriodicalIF":4.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA-metabarcoding of cyanobacteria and microalgae in chernozem soils of temperate continental climate of the forest-steppe zone of Eurasia under different degrees of agrotechnology intensification.","authors":"Vyacheslav Lukyanov, Lira Gaysina, Yurij Bukin, Prabhaharan Renganathan, Alexey Tupikin","doi":"10.1007/s11274-024-04133-5","DOIUrl":"10.1007/s11274-024-04133-5","url":null,"abstract":"<p><p>Chernozem soil is a valuable resource and contains a great diversity of microorganisms that play a global role in the process of soil formation, the species diversity of which has changed over the last five years under the influence of different agrotechnologies. For the first time, under the conditions of the Central Chernozem region, grain and fallow crop rotation, studies using the DNA-metabarcoding method were carried out to study the taxonomic structure of bacteria, fungi, cyanobacteria, and microalgae communities in the arable horizon of typical medium loamy chernozem under winter wheat cultivation. A comparative analysis of the composition of the genotypes showed significant differences in the presented level of mineral nutrition of the soil NPK (60) and NPK (100) compared with the control variant. After processing the 16S and 18S rRNA datasets, a similar trend of decreasing numbers of pro- and eukaryotic species was found from 6296 (control without MF) to 5310 with NPK (60) and to 4643 with NPK (100), respectively. The Chao1 index indicated that the expected diversity within the prokaryotic group was higher in the control without MF at 211, but decreased to 182 and 193 with NPK (60) and NPK (100) fertilizers, respectively. Analysis of the eukaryotic group revealed a 2.6- and 2.9-fold decrease in diversity by class and genus, respectively, depending on the nutritional levels in agrotechnologies, owing to the use of MF. In the prokaryotic community, Alphaproteobacteria microorganisms predominated at an amount of 14.20-14.46%, with Cyanophyceae accounting for 5.2-9.9%. The diversity of eukaryotes was smaller than the number of classes of prokaryotes; the main dominant were Zygnematophyceae 19.5-41%, Chlorophyceae occupied 10.4-15.8%. On the other hand, the doses of fertilizers used contributed to the emergence of dominant species adapted to high doses of mineral nutrients for plants.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"351"},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant growth promoting bacteria promote rice growth cultivated in two different sandy soils subjected distinct climates conditions.","authors":"Salvador Ferreira de Holanda, Emilio Berghahn, Luciano Kayser Vargas, Camille Eichelberger Granada","doi":"10.1007/s11274-024-04161-1","DOIUrl":"10.1007/s11274-024-04161-1","url":null,"abstract":"<p><p>Sandy soils contain around 70% sand in their composition, making them highly fragile and susceptible to land degradation. Practices such as no-tillage cultivation, the use of bioinoculants, and the application of organic amendments can restore the organic matter in these soils, ensuring sustainable production. In this context, this work aimed to study the microbiological aspects of two sandy soil areas (Brazilian Northeast and South) under contrasting climatic conditions (tropical and temperate). With this purpose, prokaryotic communities were evaluated, and the plant growth-promoting potential of isolated bacteria was assessed by rice inoculation in sandy soil. Despite the high sand content in both soils, soil from the NE was related to the highest phosphorous, calcium, potassium, copper, sodium, zinc, magnesium, and manganese contents, organic matter percentage, and pH. The Shannon diversity index indicated that prokaryotic communities in NE were more diverse than in SU, and PCA revealed that microbial composition exhibited distinct patterns. The rice inoculation experiments were executed to verify if the bacterial isolates displayed a similar growth promotion potential when inoculated in sandy soil areas subjected to different climatic conditions. When all PGP characteristics evaluated were pooled in a PCA, a similar pattern was observed for SU and NE. Burkholderia sp. SU94 was related to highest PGP characteristics evaluated. Paraburkholderia sp. NE32 showed similar results to those of the non-inoculated control. This similar effect of rice growth in the Northeast and South of Brazil suggests that isolate SU94 adapts to different environmental conditions.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"352"},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AVR/I/SSAPDB: a comprehensive & specialised knowledgebase of antimicrobial peptides to combat VRSA, VISA, and VSSA.","authors":"Rajat Kumar Mondal, Debayan Karmakar, Oshin Pal, Sintu Kumar Samanta","doi":"10.1007/s11274-024-04162-0","DOIUrl":"10.1007/s11274-024-04162-0","url":null,"abstract":"<p><p>The rise of multi-drug resistant (MDR) bacteria, especially strains of Staphylococcus aureus like Vancomycin-resistant S. aureus (VRSA), Vancomycin-intermediate S. aureus (VISA), and Vancomycin-susceptible S. aureus (VSSA), poses a severe threat to global health. This situation underscores the urgent need for novel antimicrobial agents to combat these resistant strains effectively. Here, we are introducing the Anti-Vancomycin-Resistant/Intermediate/Susceptible Staphylococcus aureus Peptide Database (AVR/I/SSAPDB), a manually curated comprehensive and specialised knowledgebase dedicated to antimicrobial peptides (AMPs) that target VRSA, VISA, and VSSA with clinical and non-clinical significance. Our database sources data from PubMed, cataloging 491 experimentally validated AMPs with detailed annotations on peptides, activity, and cross-references to external databases like PubMed, UniProt, PDB, and DrugBank. AVR/I/SSAPDB offers a user-friendly interface with simple to advanced and list-based search capabilities, enabling researchers to explore AMPs against VRSA, VISA, and VSSA. We are hoping that this resource will be helpful to the scientific community in developing targeted peptide-based therapeutics, providing a crucial tool for combating VRSA, VISA, and VSSA, and addressing a major public health concern. AVR/I/SSAPDB is freely accessible via any web-browser at URL: https://bblserver.org.in/avrissa/ .</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"348"},"PeriodicalIF":4.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manipulating the nucleolar serine-rich protein Srp40p in Saccharomyces cerevisiae may improve isobutanol production.","authors":"Aili Zhang, Yunpeng Ding, Wenju Shao","doi":"10.1007/s11274-024-04150-4","DOIUrl":"10.1007/s11274-024-04150-4","url":null,"abstract":"<p><p>Isobutanol represents a promising second-generation biofuel. Saccharomyces cerevisiae can produce minor quantities of isobutanol as a byproduct. Increasing yeast tolerance to isobutanol is a crucial step toward achieving higher production levels. Previously, we discovered that expression of the srp40 gene could increase S. cerevisiae isobutanol tolerance. In this study, we explored the impact of overexpressing srp40 on isobutanol production. We used the CEN/ARS plasmid YCplac22-srp40 to overexpress srp40 in S. cerevisiae strain W303-1A. The resulting strain was named W303-1A-srp40. We subsequently performed metabolic engineering of isobutanol synthesis by overexpressing ILV2, ILV3 and ARO10 in W303-1 A-srp40. The resulting strain was named 303V2V3A10-22-srp40. Our findings revealed that, compared with the control strain, the 303V2V3A10-22-srp40 strain amplified isobutanol production by 50%. A transcriptome analysis revealed that upregulated genes associated with aminoacyl-tRNA biosynthesis or downregulated genes associated with phenylalanine, tyrosine, and tryptophan biosynthesis might yield increased isobutanol production in 303V2V3A10-22-srp40. Moreover, the decreases in the biosynthesis of amino acids and oxidative phosphorylation might play pivotal roles in the increased isobutanol tolerance of strain W303-1A-srp40. In summary, the overexpression of srp40 could increase isobutanol production and tolerance in S. cerevisiae. This study offers novel insights regarding strategies for increasing isobutanol production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 11","pages":"349"},"PeriodicalIF":4.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}