World journal of microbiology & biotechnology最新文献_第5页

Glycolipopeptide biosurfactants from Serratia marcescens exhibit broad-spectrum antimicrobial activity for sustainable biocontrol of multidrug-resistant pathogens. 粘质沙雷氏菌的糖脂肽生物表面活性剂具有广谱抗菌活性，可用于耐多药病原菌的可持续生物防治。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-26 DOI: 10.1007/s11274-025-04565-7

Amir Mahgoub Awadelkareem, Arif Jamal Siddiqui, Mitesh Patel, Syed Amir Ashraf, Insaf Bahrini, Yazeed Albulaihed, Mohammad Saquib Ashraf, Shahida Banu Shamsuddeen, Mohd Adnan

{"title":"Glycolipopeptide biosurfactants from Serratia marcescens exhibit broad-spectrum antimicrobial activity for sustainable biocontrol of multidrug-resistant pathogens.","authors":"Amir Mahgoub Awadelkareem, Arif Jamal Siddiqui, Mitesh Patel, Syed Amir Ashraf, Insaf Bahrini, Yazeed Albulaihed, Mohammad Saquib Ashraf, Shahida Banu Shamsuddeen, Mohd Adnan","doi":"10.1007/s11274-025-04565-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04565-7","url":null,"abstract":"The rising prevalence of multidrug-resistant (MDR) pathogens across clinical, agricultural, and environmental settings underscores the urgent need for alternative antimicrobial agents. In this study, biosurfactants produced by Serratia marcescens were extracted, isolated, and characterized to assess their broad-spectrum antimicrobial potential. The S. marcescens-derived biosurfactant (SMBS) demonstrated strong surface activity, confirmed through oil spreading (19.4 mm), drop collapse, and a high emulsification index (%E24 of 74.8% with olive oil). Structural characterization using HPTLC, FTIR, and GC-MS suggested that SMBS is a complex glycolipopeptide comprising lipid, peptide, and carbohydrate moieties. Functionally, SMBS exhibited considerable antibacterial activity with inhibition zones of 12 mm, 11 mm, and 14 mm against Acinetobacter pittii, Proteus vulgaris, and Staphylococcus aureus, respectively. Antifungal assays revealed notable inhibition of Aspergillus niger (2 mm) Candida albicans (6 mm), Fusarium proliferatum (16 mm), and Penicillium purpurogenum (20 mm). While SMBS showed significant activity, it was comparatively less effective than streptomycin in most cases. These findings highlight the potential of SMBS as a sustainable, multifunctional biocontrol agent suitable for use in healthcare, agriculture, and environmental management. Its broad-spectrum efficacy, structural complexity, and biodegradability offer an attractive alternative to conventional antimicrobials. However, challenges such as large-scale purification, production cost, and the need for detailed toxicity and mechanism-of-action studies remain. Addressing these aspects will be essential for advancing SMBS toward clinical and commercial applications.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"330"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-galactanase: an effective tool for the degradation of plant β-ᴅ-galactan. β-半乳糖酶：降解植物β-半乳糖的有效工具。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-26 DOI: 10.1007/s11274-025-04535-z

Yanbo Hu, Yuzhu Shen, Yudi Song, Yue Yang, Yakun Shan, Ruining Zhang, Jun Zhao

{"title":"β-galactanase: an effective tool for the degradation of plant β-ᴅ-galactan.","authors":"Yanbo Hu, Yuzhu Shen, Yudi Song, Yue Yang, Yakun Shan, Ruining Zhang, Jun Zhao","doi":"10.1007/s11274-025-04535-z","DOIUrl":"https://doi.org/10.1007/s11274-025-04535-z","url":null,"abstract":"βGalactanases specifically hydrolyze the interglycosidic bonds of galactose residues in galactan and arabinogalactan, and they are widely distributed in both prokaryotes and eukaryotes. In this review, β-galactanases, including β-1,3-galactanase, β-1,4-galactanase, and β-1,6-galactanase, are summarized from both theoretical and applied perspectives. Studies have demonstrated that β-galactanases primarily belong to the glycoside hydrolase (GH) families 30, 43, and 53. The catalytic mechanisms of the GH30 and GH53 families follow a typical retention hydrolysis mechanism, while only members of the GH43 family adhere to the inverting hydrolysis mechanism. Due to structural differences among β-galactanases, their enzymatic properties and substrate specificities also exhibit significant variations. β-Galactanases have a wide range of applications. For example, β-1,4-galactanases are extensively used in the preparation of galacto-oligosaccharides (GOS) as a food additive and as a fingerprinting tool for medicinal plants. β-1,3-Galactanases and β-1,6-galactanases are less commonly used but are highly effective tools for the analysis of arabinogalactan-II (AG-II) pectin. Furthermore, to expand the application spectrum of β-galactanases, the preparation methods and basic enzymatic properties of these enzymes are summarized. The results demonstrate that β-galactanases have broad application prospects.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"336"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the catalytic efficiency and thermostability of NADH oxidase by a multi-strategy computational approach. 用多策略计算方法提高NADH氧化酶的催化效率和热稳定性。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-26 DOI: 10.1007/s11274-025-04553-x

Taisong Shen, Yujiao Li, Hongling Shi, Baomin Luo, Zixing Dong, Yunchao Kan, Dandan Li, Lunguang Yao, Cunduo Tang

{"title":"Enhancing the catalytic efficiency and thermostability of NADH oxidase by a multi-strategy computational approach.","authors":"Taisong Shen, Yujiao Li, Hongling Shi, Baomin Luo, Zixing Dong, Yunchao Kan, Dandan Li, Lunguang Yao, Cunduo Tang","doi":"10.1007/s11274-025-04553-x","DOIUrl":"https://doi.org/10.1007/s11274-025-04553-x","url":null,"abstract":"NADH oxidase (NOX) plays a crucial role in diverse fields, including medicine, food processing, and environmental protection, by catalyzing the oxidation of NADH to NAD⁺. This reaction is essential for biosensor performance, metabolic regulation, anti-aging processes, and the degradation of industrial pollutants. In this study, a NOX (EfNOX) was obtained through gene cloning from Enterococcus faecium. However, its limited thermal stability and catalytic efficiency constrained its industrial applications. To address these limitations, rational design strategies were employed to generate a double mutant, N211M/Q293L. The mutant exhibited a half-life of 27 min at 50℃ and a specific activity of 24.9 U/mg, representing 2.08-fold and 1.87-fold improvements, respectively, over the wild-type EfNOX. These enhancements are expected to promote the applicability of EfNOX in high-temperature environments and its industrial application potential. Collectively, this work offers a practical and efficient strategy for enhancing NOX and other industrial enzymes.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"332"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-level production of Tauroursodeoxycholic acid from Taurochenodeoxycholic acid in engineered Bacillus subtilis via whole-cell biotransformation. 在工程枯草芽孢杆菌中通过全细胞生物转化，由牛磺酸去氧胆酸高水平生产牛磺酸去氧胆酸。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-26 DOI: 10.1007/s11274-025-04549-7

Lina Jin, Tengfei Niu, Rufeng Wang, Li Yang, Shujuan Zhao, Zhengtao Wang

{"title":"High-level production of Tauroursodeoxycholic acid from Taurochenodeoxycholic acid in engineered Bacillus subtilis via whole-cell biotransformation.","authors":"Lina Jin, Tengfei Niu, Rufeng Wang, Li Yang, Shujuan Zhao, Zhengtao Wang","doi":"10.1007/s11274-025-04549-7","DOIUrl":"https://doi.org/10.1007/s11274-025-04549-7","url":null,"abstract":"Bear bile powder, a valuable traditional Chinese medicinal material, is distinguished by its high content of tauroursodeoxycholic acid (TUDCA), typically present at a ratio of 0.82 to 1.76 relative to taurochenodeoxycholic acid (TCDCA). In this study, the generally recognized as safe (GRAS) bacterium Bacillus subtilis was engineered to create a two-step enzymatic pathway for TCDCA to TUDCA bioconversion, utilizing bidirectional redox enzymes 7α/7β-hydroxysteroid dehydrogenase. Ribosome binding site (RBS) optimization and glucose 1-dehydrogenase (GDH)-mediated NAD(P)H cofactor regeneration were implemented to increase TUDCA production. The engineered strains A2-2 and A2-2-5 produced 15.10 ± 0.36 g/L and 14.33 ± 1.24 g/L of TUDCA, with TUDCA/TCDCA ratios of 1.22 and 0.88, respectively, following 15 h of fermentation in a whole-cell biocatalytic system. T-7-KLCA intermediate was undetectable. This study provided a cost-effective and scalable approach for the industrial production of potential bear bile substitutes from readily available chicken bile powder using an engineered probiotic B. subtilis microbial cell factory.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"334"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen. 单核增生李斯特菌：从腐生菌到胞质毒性病原体。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-26 DOI: 10.1007/s11274-025-04545-x

Shahidul Ahmed Khan, Yeon Soo Han

{"title":"Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen.","authors":"Shahidul Ahmed Khan, Yeon Soo Han","doi":"10.1007/s11274-025-04545-x","DOIUrl":"https://doi.org/10.1007/s11274-025-04545-x","url":null,"abstract":"Listeria monocytogenes is a facultative intracellular bacterium that commonly resides in soil as a saprophyte but is capable of transitioning into a pathogen when ingested by invertebrate hosts, including humans and various animals. Listeria monocytogenes is a well-recognized foodborne pathogen responsible for diverse clinical manifestations, from mild self-limiting gastroenteritis to severe invasive diseases, such as septicemia, meningitis, encephalitis, and pregnancy-associated complications, such as spontaneous abortion. Pathogenesis is governed by sophisticated regulatory networks that orchestrate gene expression and coordinate virulence. This review provides an in-depth analysis of the microbiological traits of L. monocytogenes as a foodborne pathogen, emphasizing the virulence mechanisms of this bacterium in both vertebrate and invertebrate hosts. Key virulence factors include internalin-mediated host cell adhesion and invasion, flagella-driven motility, the positive regulatory factor A (PrfA)-regulated transcriptional control of major virulence genes, the listeriolysin O-induced disruption of phagosomal membranes, the phospholipase-mediated evasion of host defense mechanisms, actin polymerization-dependent intracellular and intercellular spread, sigma factor-driven stress responses, biofilm formation and the development of resistance to antimicrobial agents. Strategies for microbiological control of virulence are also discussed.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"344"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient fermentative production of SAM through multidimensional engineering of Saccharomyces cerevisiae. 通过酿酒酵母的多维工程高效发酵生产SAM。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-26 DOI: 10.1007/s11274-025-04319-5

Yuanshan Wang, Liangzhuang Tan, Meijing Li, Lang Wang, Zuoyu Huang, Puhong Yi, Chunyue Weng, Zhongce Hu, Zhiqiang Liu, Yuguo Zheng

{"title":"Efficient fermentative production of SAM through multidimensional engineering of Saccharomyces cerevisiae.","authors":"Yuanshan Wang, Liangzhuang Tan, Meijing Li, Lang Wang, Zuoyu Huang, Puhong Yi, Chunyue Weng, Zhongce Hu, Zhiqiang Liu, Yuguo Zheng","doi":"10.1007/s11274-025-04319-5","DOIUrl":"https://doi.org/10.1007/s11274-025-04319-5","url":null,"abstract":"S-Adenosyl-L-methionine (SAM), an important biochemical substance, is experiencing increased demand due to its versatile applications in the medical field. Although Saccharomyces cerevisiae has been adapted as a promising platform for SAM production, the issue of SAM productivity is still very interesting. In this study, a systematic approach that consists of genome shuffling, genetic engineering, and fermentation process optimization strategies was established. Firstly, haploids of mutant T11-1 and P15-33 were prepared and treated with ultraviolet irradiation (UV) and atmospheric and room temperature plasma (ARTP) compound mutagenesis, which aids to increase SAM production to 1.25, 1.34 g/L, respectively. Genome shuffling was performed with cycloheximide-resistant protoplast of mutant T5-8-1 and hygromycin B-resistant protoplasts of mutant P4-2-4. The resulting fusant TRP60, with a SAM titer of 1.36 g/L, was subjected to transcriptome analysis. Subsequently, hemoglobin from vitreoscilla has been introduced into fusant TRP60 to increase ATP supply and then strengthening the synthesis pathway of SAM, which significantly elevated SAM titer to 2.06 g/L. Finally, the SAM titer of fusant TRP60-vgb-SAM2 reached 14.22 g/L in a 5 L bioreactor with an optimized fed-batch fermentation. Therefore, fusant TRP60-vgb-SAM2 may serve as a candidate for industrial production of SAM.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"345"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial variation and composition of bacterial communities in a cave system from Northern Mexico. 墨西哥北部洞穴系统中细菌群落的空间变化和组成。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-15 DOI: 10.1007/s11274-025-04540-2

Dannia Merari Manjarrez-Rascón, Zilia Yanira Muñoz-Ramirez, Javier Carrillo-Campos, Ismael Ortiz-Aguirre, Román González-Escobedo

{"title":"Spatial variation and composition of bacterial communities in a cave system from Northern Mexico.","authors":"Dannia Merari Manjarrez-Rascón, Zilia Yanira Muñoz-Ramirez, Javier Carrillo-Campos, Ismael Ortiz-Aguirre, Román González-Escobedo","doi":"10.1007/s11274-025-04540-2","DOIUrl":"10.1007/s11274-025-04540-2","url":null,"abstract":"Caves are oligotrophic, light-deprived ecosystems that host highly specialized and diverse microbial communities playing critical roles in biogeochemical cycling and ecological stability. In this study, we present the first comprehensive characterization of bacterial communities within the Nombre de Dios Caves in Chihuahua, northern Mexico. Using full-length 16S rRNA gene sequencing with PacBio HiFi technology, we analyzed bacterial diversity across five cave chambers. A total of 36 bacterial phyla, including 822 genera, were identified, with Bacteroidota, Pseudomonadota, and Bacillota being the most dominant. At the genus level, JC017, Staphylococcus, Crocosphaera, and Enterobacter were among the most abundant taxa. Alpha diversity analyses revealed significant differences in Shannon diversity and evenness, suggesting that local chamber-specific factors may influence bacterial richness and community balance. Similarly, beta diversity analyses showed clear compositional differences between chambers, particularly in the palm tree and bathroom chambers, the latter being notably isolated from tourist access. This spatial variation highlights the influence of environmental heterogeneity and human presence on microbial community structure. The microbial assemblages included ecologically important genera such as Crocosphaera and Nodosilinea, known for nitrogen fixation, exopolysaccharide production, and biofilm development. Clinically relevant genera, including Escherichia, Klebsiella, Staphylococcus, and Enterococcus, were also detected, suggesting the possible introduction of allochthonous microbes through anthropogenic activity. These findings underscore the ecological and public health significance of cave microbiomes and offer valuable insights for microbial monitoring and conservation strategies in fragile karst environments.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"326"},"PeriodicalIF":4.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing glycerol for secondary metabolite biosynthesis in microorganisms. 利用甘油进行微生物次生代谢物的生物合成。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-15 DOI: 10.1007/s11274-025-04537-x

Kouhei Kamasaka, Luiz Marcello, Lucília Domingues, Tomohisa Hasunuma

{"title":"Harnessing glycerol for secondary metabolite biosynthesis in microorganisms.","authors":"Kouhei Kamasaka, Luiz Marcello, Lucília Domingues, Tomohisa Hasunuma","doi":"10.1007/s11274-025-04537-x","DOIUrl":"10.1007/s11274-025-04537-x","url":null,"abstract":"The increase in glycerol availability as a byproduct of biodiesel production has attracted attention in its utilization as a carbon source for microbial fermentation. Compared with traditional carbon sources such as glucose, glycerol enhances the production of various secondary metabolites such as aromatic compounds, polyols, and lipids in microorganisms such as Komagataella phaffii and Yarrowia lipolytica. For instance, in K. phaffii, glycerol increased the titers of p-coumarate, naringenin, and resveratrol compared with glucose-based media, particularly when combined with specific amino acid supplementation. This review presents recent advancements in the production of secondary metabolites from glycerol. The metabolic pathways involved in glycerol utilization and secondary metabolite biosynthesis have been examined with insights into the regulation of these processes. The potential applications of glycerol-derived secondary metabolites in the pharmaceutical, food, and biofuel industries have been discussed. Finally, the challenges such as strain robustness, variability in crude glycerol quality, and downstream processing have been addressed with focus on recent innovations in metabolic engineering, redox tuning, and bioprocess control. Overall, this review highlights the potential of glycerol in the context of these advancements as a sustainable and cost-effective feedstock for the next-generation microbial biomanufacturing of high-value secondary metabolites.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"325"},"PeriodicalIF":4.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of microbial communities with metabolites and enzyme activities in different colors of high-temperature Daqu. 不同颜色高温大曲中微生物群落与代谢产物及酶活性的关系

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-12 DOI: 10.1007/s11274-025-04562-w

Peilin Que, Shangjie Yao, Jianfeng Hu, Feng Hu, Diqiang Wang, Wei Huang

{"title":"Associations of microbial communities with metabolites and enzyme activities in different colors of high-temperature Daqu.","authors":"Peilin Que, Shangjie Yao, Jianfeng Hu, Feng Hu, Diqiang Wang, Wei Huang","doi":"10.1007/s11274-025-04562-w","DOIUrl":"10.1007/s11274-025-04562-w","url":null,"abstract":"The distinctions among three types of high-temperature Daqu (yellow, white, and black Daqu) remain insufficiently characterized. In this study, we collected Daqu samples at different fermentation timepoints (days 8, 15, and 40) to analyze their enzyme activities, microbial diversity, and metabolomic profiles. Enzymatic analysis revealed that white Daqu exhibited significantly higher amylase and neutral protease activities compared with black Daqu and yellow Daqu. Through Illumina MiSeq sequencing, five dominant bacterial genera and six dominant fungal genera were identified in fermentation process. Microbial community analysis revealed that Bacillus and Scopulibacillus were the dominant bacterial genera in white Daqu, whereas Kroppenstedtia, Saccharopolyspora, Thermoascus, Saccharomyces, and Aspergillus dominated black Daqu. Non-targeted metabolomics indicated that carboxylic acids and derivatives, fatty acyls, organooxygenated compounds, and substituted benzene derivatives represented the majority of the differentially abundant metabolites. Moreover, black Daqu contained the highest number of differentially abundant metabolites compared to yellow and white Daqu. Correlation analysis demonstrated that Bacillus and Scopulibacillus showed significant positive correlations with neutral protease and amylase activities. Furthermore, Saccharopolyspora and Thermoascus exhibited strong positive correlations with metabolites involved in arginine and proline metabolism. This study provides valuable insights for optimizing traditional fermented food industry.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"324"},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protecting DNA and improving freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 through metabolism of hypoxanthine. 利用次黄嘌呤代谢保护植物乳杆菌LIP-1的DNA，提高其冻干存活率。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-12 DOI: 10.1007/s11274-025-04532-2

Xingkun Guo, Qiaoling Zhang, Rongze Ma, Ruoru Zhuang, Jing Li, Shuyi Jiao, Jingjing E, Junguo Wang, Rula Sa

{"title":"Protecting DNA and improving freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 through metabolism of hypoxanthine.","authors":"Xingkun Guo, Qiaoling Zhang, Rongze Ma, Ruoru Zhuang, Jing Li, Shuyi Jiao, Jingjing E, Junguo Wang, Rula Sa","doi":"10.1007/s11274-025-04532-2","DOIUrl":"10.1007/s11274-025-04532-2","url":null,"abstract":"Freeze drying is widely employed in the industrial production of probiotic powders. However, it often results in a marked reduction in strain viability due to exposure to low temperatures and dehydration, with DNA damage being a key contributor to cell death. Given the established link between purine metabolism and DNA repair, this study explored the effects of exogenous added hypoxanthine supplementation on freeze-drying survival and its underlying mechanisms, as well as its impact on the room-temperature storage stability of Lactiplantibacillus plantarum LIP-1. Our results showed that supplementation with 0.03 g/L hypoxanthine in MRS culture medium significantly improved both the freeze-drying survival rate and room-temperature stability of L. plantarum LIP-1 compared with the control group (P < 0.05). Mechanistically, the strain metabolized hypoxanthine to enhance the synthesis of inosine monophosphate (IMP) which in exerted feedback inhibition on the conversion of glutamine to IMP, leading to intracellular accumulation of its precursor substance, glutamate. Concurrently, hypoxanthine addition facilitated the establishment of a low-pH environment during bacterial growth, promoting the conversion of glutamate to γ-aminobutyric acid (GABA). This conversion irreversibly consumed intracellular H+, and given the alkaline nature of GABA, further elevated the intracellular pH, thereby mitigating DNA damage. This study presents the first evidence of hypoxanthine's beneficial role in improving both freeze-drying resistance and storage stability in Lactobacillus. These findings provide a novel strategy to optimize freeze-drying processes for producing highly viable probiotic powders.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"323"},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0