World journal of microbiology & biotechnology最新文献

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Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification. 用重组纤维生物水解酶(GH7)强化拉森氏菌分泌组,实现高效生物质糖化。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-07 DOI: 10.1007/s11274-025-04473-w
Yashika Raheja, Varinder Singh, Vivek Kumar Gaur, Gaurav Sharma, Adrian Tsang, Bhupinder Singh Chadha
{"title":"Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification.","authors":"Yashika Raheja, Varinder Singh, Vivek Kumar Gaur, Gaurav Sharma, Adrian Tsang, Bhupinder Singh Chadha","doi":"10.1007/s11274-025-04473-w","DOIUrl":"10.1007/s11274-025-04473-w","url":null,"abstract":"<p><p>GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 could fill this gap and how it stacks up against the industrial workhorse strain Trichoderma reesei Cel7A, we codon optimized the R. emersonii gene (Rem_GH7CBHI), expressed it in Pichia pastoris and purified the recombinant enzyme for structural and functional analysis. The 57 kDa protein retains the canonical GH7 βsandwich tunnel, but an AlaforTyr substitution leaves the channel more open than that of T. reesei, potentially easing substrate entry. Consistent with this architecture, Rem_GH7CBHI binds cellotriose tightly and exhibits a low Kₘ of 0.25 mM. Biochemical characterization revealed the optimal activity at pH 5.0, 60 °C and retaining about 60% activity after 1 h at 70 °C. Adding Rem_GH7CBHI together with an endogenous AA9 LPMO to the native R. emersonii secretome (M36) boosted saccharification of steamacidpretreated rice straw to levels comparable with commercial Cellic CTec3 at the same total protein loading. These results position Rem_GH7CBHI as a thermostable, highaffinity alternative to T. reesei Cel7A and a costeffective addition to tailored enzyme cocktails for highsolids lignocellulose biorefineries.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"253"},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of microbial community structures in rhizosphere soils of different plants in riverine wetland. 河流湿地不同植物根际土壤微生物群落结构比较
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-05 DOI: 10.1007/s11274-025-04423-6
Jiaying Zhao, Biao Liu, Yiran Hou, Liujie Zheng, Xixi Kong, Changrui Zhou, Junfeng Wu, Xiang Guo, Yimei Ying
{"title":"Comparison of microbial community structures in rhizosphere soils of different plants in riverine wetland.","authors":"Jiaying Zhao, Biao Liu, Yiran Hou, Liujie Zheng, Xixi Kong, Changrui Zhou, Junfeng Wu, Xiang Guo, Yimei Ying","doi":"10.1007/s11274-025-04423-6","DOIUrl":"10.1007/s11274-025-04423-6","url":null,"abstract":"<p><p>Bacterial communities play an important role in nutrient cycling in riverine wetland ecosystems, and the study of rhizosphere microbial community composition of different plants is of great scientific significance in revealing the relationship between microorganisms and plant interactions in riverine wetlands. In this study, samples were collected from four plant congeries dominated by Artemisia argyi (AH), Acorus calamus (CP), Miscanthus sacchariflorus (DC), and Paspalum distichum (QB), respectively. High-throughput sequencing technology was employed to investigate the rhizosphere microbial community structures associated with these distinct plant species. The results showed that soil water content could effectively regulate the functional diversity of soil microbial communities. Different plant congeries types significantly affected soil bacterial community diversity, and at the phylum level, both Proteobacteria and Acidobacteria were absolutely dominant groups, followed by Bacteroidetes. At the genus level, Flavobacterium, Sphingomonas, Gp6 and Gp4 were the dominant bacteria in the rhizosphere soil of plants along the river. Soil pH, water content, NO<sub>3</sub>-N, NO<sub>2</sub>-N and TOC were the main factors affecting the composition of microbial communities. This study broadens our understanding of the composition of rhizosphere microbial communities in the river buffer zone, and provide data support for river ecological environment protection.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"250"},"PeriodicalIF":4.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteomic analysis of Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 responding to hyper- and hyposalinity stress. 南极海冰酵母黏胶红酵母AN5对高、低盐度胁迫响应的蛋白质组学分析。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-05 DOI: 10.1007/s11274-025-04472-x
Guangfeng Kan, Yan Zhang, Hong Zhang, Kai Yu, Yanxiao Jiang, Jie Jiang, Lina Qiao, Cuijuan Shi
{"title":"Proteomic analysis of Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 responding to hyper- and hyposalinity stress.","authors":"Guangfeng Kan, Yan Zhang, Hong Zhang, Kai Yu, Yanxiao Jiang, Jie Jiang, Lina Qiao, Cuijuan Shi","doi":"10.1007/s11274-025-04472-x","DOIUrl":"10.1007/s11274-025-04472-x","url":null,"abstract":"<p><p>Antarctic sea-ice microorganism lives in polar sea-ice channels, in which the salinity can be three times that of seawater in winter and close to freshwater in summer. Here, we investigated the survival and adaptive strategies of Antarctic yeast Rhodotorula mucilaginosa AN5 treated individually with high (150‰) and low (0‰) salinity to simulate the natural environment. The results showed that salinity changes did not significantly affect the growth of yeast AN5. Scanning electron microscope (SEM) observation revealed that the cell size increased under both hyper- and hyposalinity compared to control, and more extracellular substances were observed, especially under hypersalinity stress. Biochemical factors determination indicated that after high salinity exposure, the increased antioxidant systems eliminated the induced ROS, while under low salinity stress, ROS and the antioxidant system were decreased. The proteomic analysis based on iTRAQ and PRM technology revealed that 86 proteins were up-regulated and 388 were down-regulated. Under high salinity stress, the most predominant enrichment pathway was nucleocytoplasmic transport. In addition, high salinity exposure inhibited fatty acid metabolism, the one-carbon pool by folate, and ubiquitin-mediated proteolysis. Under low salinity stress, the glyoxylate cycle pathway was induced, and aldolase was down-regulated, indicating that the energy demand in yeast survival was supplied by the catabolism of lipids. Meanwhile, low salinity also limited the synthesis of RNA, DNA, and protein by inhibiting the ribosome biogenesis and pyrimidine metabolism pathways. Generally, these results provide comprehensive insights into the molecular mechanisms underlying the adaptation of Antarctic sea-ice yeast R. mucilaginosa to high and low salt stress.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"249"},"PeriodicalIF":4.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing Pseudodesulfovibrio sp., a sulfate-reducing bacterium for the sustainable synthesis of cerium sulfide. 利用硫酸盐还原菌假脱硫弧菌可持续合成硫化铈。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-05 DOI: 10.1007/s11274-025-04462-z
Sonal Shete, Neelam Kapse, Prashant K Dhakephalkar
{"title":"Harnessing Pseudodesulfovibrio sp., a sulfate-reducing bacterium for the sustainable synthesis of cerium sulfide.","authors":"Sonal Shete, Neelam Kapse, Prashant K Dhakephalkar","doi":"10.1007/s11274-025-04462-z","DOIUrl":"10.1007/s11274-025-04462-z","url":null,"abstract":"<p><p>The present study investigates a novel sulfate-reducing bacterium, Pseudodesulfovibrio sp. MCM B-508, isolated from produced water, for its ability to produce cerium sulfide, an industrially valuable rare earth colorant. The study demonstrates a microbial route for pigment synthesis by cloning and expressing dissimilatory sulfite reductase (dsrAB) genes from this bacterium in Escherichia coli. The enzyme dissimilatory sulfite reductase shared a maximum of 94.09% homology with published dissimilatory sulfite reductase sequences from the GenBank database, highlighting its novelty. Using molecular engineering techniques, including chaperone co-expression with the pGro7 vector, the sulfate conversion efficiency was significantly enhanced to 71.23%, overcoming limitations in sulfate-reducing bacterial processes. The research successfully converted cerium sulfate to the desired gamma-form cerium sulfide (Ce<sub>2</sub>S<sub>3</sub>) through a recombinant E. coli strain, achieving a more efficient and eco-friendly production method compared to conventional chemical synthesis approaches. X-ray diffraction analysis confirmed the formation of the targeted pigment, with major peaks at 25.3 and 32.8° 2θ, demonstrating the potential for an industrially viable, sustainable pigment production process.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"251"},"PeriodicalIF":4.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-throughput screening identifies novel chemical scaffolds targeting Leishmania donovani parasites. 高通量筛选鉴定针对多诺瓦利什曼原虫寄生虫的新型化学支架。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-04 DOI: 10.1007/s11274-025-04441-4
Diksha Kumari, Vishwani Jamwal, Tashi Palmo, Anuj Kumar, Kuljit Singh
{"title":"High-throughput screening identifies novel chemical scaffolds targeting Leishmania donovani parasites.","authors":"Diksha Kumari, Vishwani Jamwal, Tashi Palmo, Anuj Kumar, Kuljit Singh","doi":"10.1007/s11274-025-04441-4","DOIUrl":"10.1007/s11274-025-04441-4","url":null,"abstract":"<p><p>High-throughput screening (HTS) is one of the critical strategies that can accelerate the process of drug discovery by exploring small drug-like molecule libraries. HTS noticeably contributes to the identification of potential hits using various automation tools and biological assays. Leishmaniasis, a neglected tropical disease, is one of the most deadly protozoan parasitic infections causing significant global burden in certain poverty-stricken countries. Given the paucity of effective chemotherapeutic agents available for the treatment of this life-threatening infection, the discovery of novel leishmanicidal scaffolds through innovative strategies is pivotal to combating this parasitic disease. In the present study, we have employed the HTS strategy to scrutinize 10,000 drug-like molecules from the in-house ChemDiv library to identify potent hits against Leishmania donovani. Noticeably, 99 molecules showed > 80% inhibitory effect against the L. donovani parasites at 50 µM concentration, and among them, four molecules displayed a 50% inhibitory concentration (IC<sub>50</sub>) value < 10 µM along with a favorable selectivity index (> 10). Scanning electron microscopy analysis suggests that these potent hits cause considerable morphological alterations. The predicted ADME or pharmacokinetics study of these potent hit molecules indicates that all the hits have considerable drug-likeness properties and showed a low risk of adverse effects, along with a predicted high level of oral bioavailability. Thus, these promising hits identified by the HTS strategy can serve as an initial basis for consequent medicinal chemistry endeavors aimed at unraveling a new series of anti-leishmanial agents.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"247"},"PeriodicalIF":4.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biosynthesis of D-threitol from biomass-derived xylose by engineering Trichosporonoides oedocephalis. 生物质木糖合成d -苏糖醇的工程研究。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-04 DOI: 10.1007/s11274-025-04425-4
Jingyi Xu, Yue Tang, Yuqi Peng, Zhi Chen, Xin Ju, Liangzhi Li
{"title":"Biosynthesis of D-threitol from biomass-derived xylose by engineering Trichosporonoides oedocephalis.","authors":"Jingyi Xu, Yue Tang, Yuqi Peng, Zhi Chen, Xin Ju, Liangzhi Li","doi":"10.1007/s11274-025-04425-4","DOIUrl":"10.1007/s11274-025-04425-4","url":null,"abstract":"<p><p>Xylose is the major pentose and the second most abundant sugar in the lignocellulosic feedstock. Metabolic engineering approaches have been employed to enhance the utilization rate of xylose in yeast. We previously modified Trichosporonoides oedocephalis ATCC 16958 and produced sugar alcohols, including xylitol and erythritol. D-threitol is a rare sugar that cannot be obtained directly from xylose conversion through fermentation. In this study, T. oedocephalis gained xylose assimilation activity by introducing ribulose-5-phosphate isomerase B from Ochrobactrum sp. and xylitol dehydrogenase from the yeast Scheffersomyces stipitis CBS 6054 for D-threitol production. Optimization of the composition of the medium ultimately led to an increase in the yield of D-threitol to 5.18 g/L. In addition, this study also analyzed the kinetics of the fermentation process, including cell growth, D-threitol biosynthesis and xylose consumption. Cell growth conformed to the logistics curve, and D-threitol synthesis and substrate consumption conformed to the Luedeking-Piret curve showing a growth-partially correlated type. This study demonstrated the potential of engineered yeast to synthesize high value-added products from biomass-derived xylose.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"248"},"PeriodicalIF":4.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seed inhabiting bacterial endophyte Bacillus amyloliquefaciens (SS7) defends sorghum Sudan grass seedlings from a phytopathogen Rhizoctonia solani. 种子内生细菌解淀粉芽孢杆菌(SS7)保护高粱苏丹草幼苗免受植物病原体索拉根丝核菌的侵害。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-03 DOI: 10.1007/s11274-025-04455-y
Kanchan Kumar, Deepak Kumar, Gaurav Pal, Satish K Verma
{"title":"Seed inhabiting bacterial endophyte Bacillus amyloliquefaciens (SS7) defends sorghum Sudan grass seedlings from a phytopathogen Rhizoctonia solani.","authors":"Kanchan Kumar, Deepak Kumar, Gaurav Pal, Satish K Verma","doi":"10.1007/s11274-025-04455-y","DOIUrl":"10.1007/s11274-025-04455-y","url":null,"abstract":"<p><p>Seeds naturally harbour bacterial endophytes that act as primary symbionts of plants. This study aimed to isolate and characterise seed inhabiting bacterial endophytes of sorghum sudan grass and evaluate the most active endophyte, Bacillus amyloliquefaciens-SS7 for protecting seedlings against Rhizoctonia solani infection. Seven bacterial endophytes (SS1-SS7) were isolated from surface sterilized sorghum sudan grass seeds and identified via 16 S rRNA sequencing. All isolates were screened for plant growth promoting and antifungal activities. B. amyloliquefaciens-SS7 exhibited the highest siderophore production and strong antifungal activity. Gene screening confirmed the presence of antifungal lipopeptide genes (Iturin A and Bacillomycin D) in B. amyloliquefaciens. Extracted lipopeptides from B. amyloliquefaciens strongly inhibited R. solani in disc diffusion assays. Bright field and fluorescence microscopy revealed that lipopeptides caused structural deformations and cell death in R. solani. Microcosm assays demonstrated significant protection of seedlings inoculated with B. amyloliquefaciens from Rhizoctonia infection. Histological studies confirmed overproduction of superoxide ions and cell death in seedlings infected with R. solani. Biochemical analysis showed increased levels of phenylalanine ammonia-lyase (PAL), superoxide dismutase (SOD), and peroxidase (POX) in treated seedlings compared controls. Infections caused by R. solani targeted the collar region of seedlings, causing damping-off in those lacking the endophyte B. amyloliquefaciens. This study concludes that sorghum sudan grass seeds contain bacteria like B. amyloliquefaciens-SS7, which are crucial in protecting seedlings from R. solani. This research is the first to report on the role of seed endophytic bacteria (SEB) in the protecting sorghum sudan grass against R. solani.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"246"},"PeriodicalIF":4.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and comprehensive genomic characterization of cellulolytic Bacillus species reveal unique CAZyme profiles for leaf litter degradation and compost production. 纤维素分解芽孢杆菌的鉴定和综合基因组特征揭示了凋落叶降解和堆肥生产的独特酶谱。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-03 DOI: 10.1007/s11274-025-04474-9
Arvind Kumar Singh, Subhojit Datta, Girindrani Dutta, Tinku Goswami, Pranit Mukherjee, Bijan Majumdar, Gouranga Kar
{"title":"Identification and comprehensive genomic characterization of cellulolytic Bacillus species reveal unique CAZyme profiles for leaf litter degradation and compost production.","authors":"Arvind Kumar Singh, Subhojit Datta, Girindrani Dutta, Tinku Goswami, Pranit Mukherjee, Bijan Majumdar, Gouranga Kar","doi":"10.1007/s11274-025-04474-9","DOIUrl":"10.1007/s11274-025-04474-9","url":null,"abstract":"<p><p>Leaf litter, abundant in lignocellulosic materials, is difficult to degrade, hindering efficient composting in agriculture. This study focused on isolating and identifying Bacillus species with strong cellulolytic activity to improve leaf litter decomposition under subtropical agro-climatic conditions. Three bacterial strains, B1S, B1M, and B1D were isolated from composted leaf litter and characterized through morphological, biochemical, and enzymatic analyses. B1S, identified as Bacillus stercoris, exhibited the highest total cellulase (0.818 ± 0.026 fpu/ml) and endoglucanase (25.674 ± 2.085 CMCase/mL) activities. B1D showed the highest β-glucosidase activity (3.561 ± 0.088 IU/mL). Optimal cellulase activity for B1S occurred at pH 5.0 and 37 °C. Inoculating compost piles with a mix of these strains significantly reduced composting time from 100 to 50 days, with a cellulose degradation rate of 45.18%. The study included whole genome sequencing of B1S (~ 4.2 Mb, 4184 coding sequences), revealing genes encoding key carbohydrate-active enzymes (CAZymes) such as glycoside hydrolases (GH5), carbohydrate-binding modules (CBM3), and auxiliary activity enzymes (AA), confirming its lignocellulolytic capacity. Taxonomic analysis using TYGS and genome-wide phylogeny validated its identity as Bacillus stercoris. Functional annotations through RAST, KEGG, and COG databases indicated enrichment in genes related to carbohydrate and amino acid metabolism, quorum sensing, and environmental adaptation. The presence of unique gene clusters and mobile genetic elements suggests genomic flexibility. These findings demonstrate the potential of Bacillus stercoris as a valuable microbial tool for sustainable composting and underscore the importance of genome-based approaches in microbial functional and taxonomic studies.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"245"},"PeriodicalIF":4.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sonoporation-enhanced the antibacterial efficacy of aminoglycosides against Gram-negative persisters. 超声波增强氨基糖苷对革兰氏阴性顽固性细菌的抑菌效果。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-02 DOI: 10.1007/s11274-025-04414-7
Hongyi Chen, Jingkai Wang, Fang Han, Chen Guo, Xinmiao Fu, Zhiqiang Zhang, Yizhi Song
{"title":"Sonoporation-enhanced the antibacterial efficacy of aminoglycosides against Gram-negative persisters.","authors":"Hongyi Chen, Jingkai Wang, Fang Han, Chen Guo, Xinmiao Fu, Zhiqiang Zhang, Yizhi Song","doi":"10.1007/s11274-025-04414-7","DOIUrl":"10.1007/s11274-025-04414-7","url":null,"abstract":"<p><p>Bacterial persisters pose a significant challenge to antibiotic therapy, often causing treatment failure and chronic infections. Improving antibiotic efficacy against persisters is thus clinically crucial. In this study, we developed a low-frequency and low-intensity ultrasound system (40 kHz, 0.2 W/cm<sup>2</sup>) to induce sonoporation in bacterial persisters. Combining ultrasound with aminoglycosides (AGs) reduced the persister ratio of Gram-negative bacteria (E. coli, P. aeruginosa, and A. baumannii) by approximately 5- to 15-fold, and notably, this approach achieves eradication below the detection threshold (< 2 CFU/mL) in MG1655 persisters. Scanning electron microscopy (SEM), N-phenyl-1-naphthylamine (NPN) staining, propidium iodide (PI) staining, ortho-nitrophenyl-β-galactoside (ONPG) assays, and inhibition zone test revealed that ultrasound primarily enhances AG uptake by increasing outer membrane permeability without significantly damaging the inner membrane, thereby potentiating antibiotic influx in Gram-negative bacteria. Based on experiments of E. coli BW25113 and its mechanosensitive (MS) channel knockout mutants, we propose that ultrasound combined with hypotonic shock enhances the permeability of MscK and YbiO channels. These findings highlight the potential of ultrasound-aminoglycoside combination therapy as a promising strategy against persistent bacterial infections, especially those caused by Gram-negative pathogens. Furthermore, this study offers new insights into bacterial persister characteristics and their susceptibility to physical and chemical interventions.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"241"},"PeriodicalIF":4.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The draft genome of 'Candidatus Phytoplasma palmae' strain LY-C2, the phytoplasma associated with coconut lethal yellowing disease, reveals insights into its biological characteristics. 与椰子致死性黄化病相关的植物原体“Candidatus Phytoplasma palmae”菌株LY-C2的基因组草图揭示了其生物学特性。
IF 4 3区 生物学
World journal of microbiology & biotechnology Pub Date : 2025-07-02 DOI: 10.1007/s11274-025-04418-3
Sara Elena Vila-Luna, Karla Gisel Carreón-Anguiano, Iván Córdova-Lara, Luis Sáenz-Carbonell, Carlos Oropeza-Salín, Blondy Canto-Canché
{"title":"The draft genome of 'Candidatus Phytoplasma palmae' strain LY-C2, the phytoplasma associated with coconut lethal yellowing disease, reveals insights into its biological characteristics.","authors":"Sara Elena Vila-Luna, Karla Gisel Carreón-Anguiano, Iván Córdova-Lara, Luis Sáenz-Carbonell, Carlos Oropeza-Salín, Blondy Canto-Canché","doi":"10.1007/s11274-025-04418-3","DOIUrl":"10.1007/s11274-025-04418-3","url":null,"abstract":"<p><p>Phytoplasmas are phloem-restricted plant pathogens that infect different plant species, causing severe symptoms in agricultural and ornamental crops. ´Candidatus Phytoplasma palmae' strain LY-C2 is associated with the coconut lethal yellowing disease, a devastating disease for coconut palm. Currently, many phytoplasma genomes have been reported in the literature. One of these corresponds to the draft genome of 'Ca. P. palmae', strain ACPD, which is associated with the Texas Phoenix Palm Decline phytoplasma and belongs to the 16SrIV-D subgroup. The genome of 'Ca. P. palmae', strain ACPD has been sequenced but not thoroughly analyzed. In this study, we present the draft genome of 'Ca. P. palmae'-LY-C2, which belongs to the 16SrIV-A subgroup. It consists of 49 scaffolds, totaling 434,453 bp in length, and contains 412 coding-genes, with 365 of them being protein-coding genes. Fifty-four effectors were predicted, 24 of which are unique to this phytoplasma and 11 share homology with effectors of the Texas Phoenix Palm Decline phytoplasma. Some effectors have protein motifs in common with those of other phytoplasmas, but five new protein motifs exclusive to 'Ca. P. palmae'-LY-C2 were identified. This study also highlights similarities and differences between 'Ca. P. palmae'-LY-C2 and other phytoplasmas, including 'Ca. P. palmae' ACPD, providing new insights into this pathogen. The availability of these draft genomes lay the groundwork for further research on the pathogenic mechanisms and evolution of 'Ca. P. palmae'.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"242"},"PeriodicalIF":4.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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