World journal of microbiology & biotechnology最新文献

{"title":"Biocontrol of plant pathogens by actinomycetes: a bibliometric analysis and systematic review.","authors":"Marcos Kovaleski, Talison Roberto Maurer, Matheus Banfi, Mateus Remor, Michel Restelatto, Rafael Rieder, Carolina Cardoso Deuner, Luciane Maria Colla","doi":"10.1007/s11274-025-04422-7","DOIUrl":"10.1007/s11274-025-04422-7","url":null,"abstract":"<p><p>Fungal disease control in agricultural crops represents one of the major challenges in modern agriculture. While synthetic fungicides are effective, their continuous use leads to pathogen resistance, environmental harm, and health risks to humans and animals. Within this context, the present study aims to review current knowledge regarding the use of actinomycetes as biological alternatives, focusing on the identification of molecules produced by these microorganisms that are responsible for biocontrol or biostimulant activity in plants. Actinomycetes are known for synthesizing bioactive compounds with antifungal, antibacterial, and plant growth-promoting properties. Among them, the genus Streptomyces stands out for its wide ecological distribution and key role in suppressing phytopathogens through the production of secondary metabolites such as antibiotics, lytic enzymes, siderophores, and resistance inducers. This review compiles findings from laboratory-based antibiosis assays, pathogen interaction studies, and the development of microbial-based inputs. The biotechnological potential of actinomycetes has led to the emergence of novel biocontrol agents that may replace conventional fungicides while also supporting bioremediation efforts, including pesticide degradation and the breakdown of toxic residues. These advances have been driven by cutting-edge biotechnological tools. Key findings highlight that actinomycetes, particularly Streptomyces spp., are effective against diverse phytopathogens, promote plant health, and contribute to environmental sustainability. In conclusion, the use of actinomycetes represents a viable and innovative alternative for sustainable disease management in agriculture.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"243"},"PeriodicalIF":4.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring host defense responses and elicitor-mediated resistance in cauliflower against black rot caused by Xanthomonas campestris pv. campestris.","authors":"Neelam Geat, Dinesh Singh, Devendra Singh, Hans Raj Mahla, Rajender Jatoth, Pedapudi Lokesh Babu","doi":"10.1007/s11274-025-04471-y","DOIUrl":"10.1007/s11274-025-04471-y","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"244"},"PeriodicalIF":4.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production, characterization, and antifungal action of a biosurfactant obtained from diazotrophic Paenibacillus sp.","authors":"Marcos André Moura Dias, Eduardo Luiz Rossini, Douglas de Britto, Pedro Martins Ribeiro Júnior, Paulo Ivan Fernandes-Júnior, Marcia Nitschke","doi":"10.1007/s11274-025-04448-x","DOIUrl":"10.1007/s11274-025-04448-x","url":null,"abstract":"<p><p>Biosurfactants (BS) are surface-active agents derived from microbes, garnering increasing industrial interest due to their environmentally friendly and sustainable nature. Although several bacterial isolates capable of producing biosurfactants have been reported, their production by diazotrophic strains remains underexplored. This study investigated the production, physicochemical properties, chemical composition and antifungal activity of BS produced by the diazotrophic bacteria Paenibacillus sp. ESA 664, isolated from the semi-arid region of Brazil. The results showed that the isolate was able to grow and produce BS in mineral media using glucose and ammonium chloride as carbon and nitrogen sources, respectively. The chemical structure of the surfactant was elucidated using liquid chromatography-mass spectrometry (LC-MS) and Fourier transform infrared spectroscopy (FTIR). Results revealed a lipopeptide structure similar to surfactin with the C15 isoform being the most abundant. The surfactin obtained showed surface tension of 24.4 mN/m, critical micellar concentration (CMC) of 3.88 mg/L, interfacial tension of 2.87 mN/m and emulsification index of 62.0%. In addition, the BS presented stability over a wide range of temperatures (-20 to121°C), pH (2 to 12), NaCl (1 to 20%), and sucrose (1 to 5%), characterizing the product as a highly effective surfactant with potential for application in various industrial fields. Moreover, the compound exhibited significant fungicidal activity against the phytopathogen Lasiodiplodia theobromae, suggesting its use in agriculture as a biocontrol agent. Future investigations should address the development of field-applicable formulations and the elucidation of its antifungal mode of action.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"239"},"PeriodicalIF":4.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell cycle arrest mitigates the loss of ribonucleic acid content in Saccharomyces pastorianus.","authors":"Hao Chen, Huajian Zhu, Chengtuo Niu, Chunfeng Liu, Qi Li","doi":"10.1007/s11274-025-04469-6","DOIUrl":"10.1007/s11274-025-04469-6","url":null,"abstract":"<p><p>Numerous studies have developed various methods to increase RNA content in yeast cells. However, the reduction of RNA content during the late growth stage appears to be unavoidable. In this study, we examined the relationship between cell cycle progression and RNA content, the trend of G2 phase cells was consistent with the RNA content. To investigate the impact of G2 arrest on RNA synthesis, we evaluated the performance of G03H8 cells by overexpressing CHK1, CKI1, or CAJ1. Finally, the RNA production was increased through the high-density fermentation of engineered strain. The results demonstrated that all three genes mitigated the reduction of RNA content during the late growth stage, with CHK1 having a minimal adverse effect on biomass reduction. In H8-CHK1 cells, the proportion of cells in the G2 phase initially decreased slightly before increasing again. A similar effect was observed when CHK1 was overexpressed in G03-△PRS5, which exhibited a higher RNA content than G03H8. To address the growth reduction associated with G2 arrest, we achieved a dry cell weight of 45.79 g/L and the final RNA production of 4.94 g/L in H8-CHK1, which had increased by 37.9% compared to fed-batch fermentation of G03H8. This study provides valuable insights into the mechanisms of RNA synthesis and offers novel strategies for enhancing RNA production in yeast cells.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"238"},"PeriodicalIF":4.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic nanoparticle-based method for microorganism concentration in sterile body fluids: Validation and clinical applications.","authors":"Bilsen Tural, Erdal Ertaş, Nurullah Uzuner, Buşra Bektaş, Emre Tural, Mehmet Çavdar, Hakan Temiz, Erdal Özbek, Servet Tural","doi":"10.1007/s11274-025-04463-y","DOIUrl":"10.1007/s11274-025-04463-y","url":null,"abstract":"<p><p>Rapid and accurate detection of microorganisms in sterile body fluids, particularly cerebrospinal fluid (CSF), is crucial for effective diagnosis and treatment. Conventional methods, such as centrifugation, may result in low microbial recovery and false negatives, limiting diagnostic accuracy. An alternative, efficient, and accessible microbial concentration method is needed. This study evaluates a nanoparticle-based microbial concentration method to enhance pathogen recovery from CSF. The method was optimized for interaction time (1 min) and nanoparticle dosage (0.01 g/mL) using standard microbial strains, including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. Its clinical performance was assessed using 800 CSF samples, and microbial identification was confirmed via MALDI-TOF MS. The method significantly improved microbial recovery across 10^-2-10^-9 CFU/mL concentrations, achieving a detection limit as low as 2 CFU/mL. Clinical validation demonstrated 100% sensitivity and specificity, detecting 15 additional true-positive cases missed by centrifugation. While centrifugation fails to detect bacteria below 10^-7 CFU/mL, our method reliably detects even at 10^-9 CFU/mL, demonstrating superior microbial enrichment, especially in low-biomass samples. This method enhances diagnostic accuracy by reducing false negatives and expediting pathogen detection. Its resource-conscious, low-cost and equipment-free nature makes it particularly beneficial for resource-limited laboratories, offering a scalable alternative for microbial concentration in CSF diagnostics.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"240"},"PeriodicalIF":4.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a fungal biodeterioration index for lignocellulosic materials relevant to the food industry.","authors":"Matilde Anaya-Villalpanda, Diana M Bosch-Crespo, Sofia Borrego-Alonso, Erasmo Gámez-Espinosa","doi":"10.1007/s11274-025-04468-7","DOIUrl":"10.1007/s11274-025-04468-7","url":null,"abstract":"<p><p>Contaminated packaging materials may represent a potential risk in food processing environments, particularly when no prior treatment is applied. This study aimed to develop a quantitative method to classify the fungal biodeterioration of lignocellulosic materials commonly used in food-related applications. Six lignocellulosic supports were evaluated: coated cardboard, uncoated cardboard, corrugated cardboard, kraft paper, cupcake wrapper, and Waltman No. 5 filter paper (used as a positive control). The materials were inoculated with a spore suspension of Aspergillus ellipticus, a strain previously characterized by its high degradative activity. Fungal growth was assessed using a conventional agar-based method and a proposed technique involving mechanical resistance analysis with a texture analyzer. A new biodeterioration index (IBF) was introduced to quantify the reduction in physical resistance of each material due to fungal activity. The method allowed the classification of both the fungal strains degradative capacity and the susceptibility of each material. Additionally, an equivalence scale was established between fungal growth on filter paper and other materials. This quantitative approach enables rapid and reproducible evaluation of biodeterioration and may serve as a useful tool for quality control, food safety assurance, and the development of bio-based packaging materials in the food industry.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"237"},"PeriodicalIF":4.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically engineering central carbon and nitrogen metabolism in Bacillus paralicheniformis for high γ-PGA production via glutamate-independent pathway.","authors":"Jishuai Qi, Rui Wang, Zirui Zhan, Jianghan Wang, Heli Shi, Xiuyun Zhao, Jun Tan, Gaofu Qi","doi":"10.1007/s11274-025-04467-8","DOIUrl":"10.1007/s11274-025-04467-8","url":null,"abstract":"<p><p>Poly-γ-glutamic acid (γ-PGA) is a biopolymer with great significance and broad applications. However, its fermentation in Bacillus strains usually requires costly glutamate supplementation. To reduce production costs, we engineered B. paralicheniformis to efficiently produce γ-PGA in a glutamate-independent manner by enhancing de novo glutamate biosynthesis via rocG-mediated pathways and alleviating carbon/nitrogen catabolite repression. In this study, we deleted three key regulatory genes: ccpA (catabolite control protein A), cggR (central glycolytic genes repressor), and tnrA (master regulator of nitrogen assimilation) to relieve repression on the TCA cycle, glycolysis, and γ-PGA biosynthesis pathways, thereby significantly increasing γ-PGA yield and productivity in B. paralicheniformis. In batch fermentation experiments, strains ΔccpA and ΔtnrA achieved γ-PGA yields of 63.86 g/L and 61.38 g/L, with productivities of 19.50 g/(L·h) and 6.93 g/(L·h) during the rapid increase period of γ-PGA production, respectively. To our knowledge, the ΔccpA strain achieved the highest γ-PGA yield and productivity among glutamate-independent producers. Both ΔccpA and ΔtnrA strains demonstrated superior efficiency in converting inexpensive NaNO₃ into high-value γ-PGA compared to the parental strain. Collectively, relieving suppression of carbon metabolism (the TCA cycle and glycolysis) and nitrogen metabolism (nitrate reduction) via genetic engineering holds significant potential for further enhancing the ability to biosynthesize γ-PGA in a glutamate-independent manner. The findings highlight the effectiveness of targeted genetic modifications in improving industrial bioprocesses for cost-efficient γ-PGA production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"236"},"PeriodicalIF":4.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunization of mice and goats with Corynebacterium pseudotuberculosis-derived rPTS, rRBN and rCP40 recombinant proteins.","authors":"Ioná Brito de Jesus, Daniela Droppa-Almeida, Caroline Ferreira, Roberto José Meyer Nascimento, Ricardo Wagner Portela, Mara Thais de Oliveira Silva, Mirna Samara Dié Alves, Guilherme Senna Dos Santos, Vasco Azevedo, Francine Ferreira Padilha, Sibele Borsuk","doi":"10.1007/s11274-025-04411-w","DOIUrl":"10.1007/s11274-025-04411-w","url":null,"abstract":"<p><p>Caseous lymphadenitis (CLA) is a chronic disease that affects sheep and goats worldwide and causes significant economic losses, and the best strategy to reduce clinical cases is vaccination. This study evaluated the immune response and protection rates of the Corynebacterium pseudotuberculosis-derived ascorbate transporter subunit (rPTS) and Ribonuclease protein (rRBN) recombinant proteins in mice and goats. Groups of eight Swiss mice each were inoculated with rPTS + Al(OH)₃ (G1), rPTS + Saponin (G2), rRBN + Al(OH)₃ (G3), rRBN + Saponin (G4), rPTS + rRBN + rCP40 + Al(OH)₃ (G5), rPTS + rRBN + rCP40 + Saponin (G6), Control Al(OH)₃ (G7) and Control Saponin (G8), respectively. The mice received two vaccine doses and were challenged with a virulent C. pseudotuberculosis strain. In addition, five groups of eight goats each were inoculated with sterile saline (G1), rPTS (G2), rRBN (G3), rCP40 (G4), and rPTS + rRBN + CP40 (G5), associated with saponin. ELISAs was used to detect specific total IgG in mice and goats, and the goat specific IFN-γ production was quantified. All immunized mice presented a peak of specific total IgG on day 60, and a higher survival rate (75%) was achieved in animals immunized with a pool of the recombinant proteins associated with saponin. Also, goats immunized with the same formulation produced significant levels of specific antibodies from day 15 post immunization and were able to significantly produce specific IFN-γ at 90 days post-immunization. Our findings suggest that the formulation containing the association of the proteins rPTS, rRBN and rCP40 associated to saponin adjuvant resulted in the best protection against the challenge in mice and was able to elicit an IFN-γ-characterized Th1 immune response in goats, and can be considered as a promising vaccine formulation for CLA.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"234"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quorum quenching of phenolic compounds from Leptolyngbya spp. MACC 32 via downregulation of the periplasmic receptor LuxP in Vibrio harveyi: Hinting a putative mechanism.","authors":"Madiyan Saranya, Byeol Ryu, Jose Seena, Bhaskaran Silpa, Ameer Ahna, C Benjamin Naman, Evgenia Glukhov, T P Sajeevan, I S Bright Singh, William H Gerwick, Joseph Valsamma","doi":"10.1007/s11274-025-04378-8","DOIUrl":"10.1007/s11274-025-04378-8","url":null,"abstract":"<p><p>Vibrio harveyi causes vibriosis, leading to high mortality and economic loss in global aquaculture. Quorum sensing (QS) driven biofilm formation makes them more resistant to various control measures. This study examined QS inhibition (QSI) of V. harveyi LB3 using marine cyanobacterial natural products. Qualitative and quantitative (88%) assays confirmed the quorum quenching (QQ) efficiency of the intracellular component of Leptolyngbya spp. MACC 32. The active fraction (E3) of the extract showed less than 50% toxicity to PmLyo-Sf9 cells and brine shrimp at 1 mg mL^-1. At MIC (0.06 mg mL^-1), E3 reduced biofilm biomass by 40-45% and metabolic activity by 10-20% without affecting bacterial growth. V. harveyi LB3 treated with E3 showed less gelatinase (64.75%), hemolytic activity (17.55%), motility (8-16%) and reduced EPS formation (45.5%), indicating its anti-virulence properties. HPLC, ¹H NMR, and GC-MS analyses confirmed that E3 contains 4-hydroxybenzoic acid (4-HBA) and 4-hydroxybenzaldehyde (4-HBAL). Synthetic controls validated the QQ of 4-HBA and 4-HBAL. Molecular docking studies hinted at the putative interaction of 4-HBA and 4-HBAL with QS proteins (LuxR, LuxP, LuxM, LuxN, LuxQ, LuxU, LuxO and LuxS) of V. harveyi. Gene expression studies showed strong LuxR, LuxP, LuxQ, and ToxR downregulation (61-97%), differential upregulation of LuxO, LuxU, aphA, qrr1-qrr5 (9-18%), and LuxS, LuxM, LuxN, Fur (2-4%). Although not confirmed with mutant strains, this study provides strong evidence that phenolic compounds from Leptolyngbya spp. MACC 32 inhibited V. harveyi LB3 QS by blocking AI-2 binding to LuxP, supporting their role in shrimp health management through the control of vibriosis.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"233"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in microbial production and consumption of nitrous oxide in agricultural soils.","authors":"Ruonan Xiong, Nan Gao, Weiqiang Huang, Xiaoyue Zhang, Weishou Shen","doi":"10.1007/s11274-025-04464-x","DOIUrl":"10.1007/s11274-025-04464-x","url":null,"abstract":"<p><p>Agricultural soil is a significant source of nitrous oxide (N₂O), a long-lived greenhouse gas. Several microbial processes in the nitrogen cycle generate N₂O but the only known sink for N₂O in the biosphere is the reduction of N₂O to N₂ catalyzed by N₂O reductase (NosZ). In this review, we summarized the latest knowledge on (i) key microbial pathways regulating N₂O production and consumption processes in agricultural soils, including nitrification and denitrification and (ii) emerging strategies for microbial-mediated mitigation of N₂O emissions from agricultural soils, including the use of nitrification and denitrification inhibitors, and the direct use of microorganisms to enhance NosZ activity. We focused on the screening and application strategies for microorganisms that can mitigate N₂O emissions. We summarized two ecological mechanisms of microbial inoculation mitigation of N₂O emissions from agricultural soils. One mechanism involved employing N₂O-reducing microorganisms containing nosZ gene to directly mitigate N₂O emissions from agricultural soils. The other mechanism utilized plant growth-promoting rhizobacteria to alter the community composition, abundance and activity of the N₂O-producing or -reducing microorganisms and indirectly mitigate N₂O emissions from agricultural soils. Additionally, we discussed the potential challenges affecting microbial inoculation technology, and explored its application prospects for reducing N₂O emissions from agricultural soils. By providing a comprehensive overview of these topics, we aimed to effectively design and apply microbial-mediated mitigation technologies to better manage and mitigate N₂O emissions from agricultural soils, ultimately contributing to global climate change mitigation efforts.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"235"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}