World journal of microbiology & biotechnology最新文献_第6页

PiPho85, a cyclin-dependent kinase of Piriformospora indica rescue colonized maize plants grown under salt stress. PiPho85是一种细胞周期蛋白依赖性激酶，可拯救盐胁迫下生长的玉米植株。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-09 DOI: 10.1007/s11274-025-04513-5

Arun Kumar Kashyap, Paras Kumar, Aayushi Bajaj, Rupesh Aggarwal, Pawan Kumar Sharma, Pooja Panwar, Shubhankar Pandey, Vikas Yadav, Ralf Oelmuller, Meenakshi Dua, Atul Kumar Johri

{"title":"PiPho85, a cyclin-dependent kinase of Piriformospora indica rescue colonized maize plants grown under salt stress.","authors":"Arun Kumar Kashyap, Paras Kumar, Aayushi Bajaj, Rupesh Aggarwal, Pawan Kumar Sharma, Pooja Panwar, Shubhankar Pandey, Vikas Yadav, Ralf Oelmuller, Meenakshi Dua, Atul Kumar Johri","doi":"10.1007/s11274-025-04513-5","DOIUrl":"10.1007/s11274-025-04513-5","url":null,"abstract":"We identified, isolated, and functionally characterized a cyclin-dependent kinase (CDK), PiPho85, from Piriformospora indica. The identified PiPho85 contains TY, PSTAIRE, protein kinase domain, and an ATP binding site which is highly conserved among the Pho85/CDK5 family protein specific for Saccharomyces cerevisiae. In a S. cerevisiae mutant strain (Δpho85), PiPho85 could regulate the acid phosphatase activity, restore the normal phenotype, confer salt- and drug-tolerance, and enable utilization of non-fermentable sugars. This suggests that PiPho85 complements the Pho85 function. The PiPho85 knock down (Kd) strain was generated by RNA interference (RNAi) and named as Kd-PiPho85-P. indica. The Kd strain was found susceptible to salt, drug and osmolarity stress and showed morphological abnormalities, abnormal-sized hyphae with large knot-like structure. This suggests that PiPho85 plays a regularity role in the physiology of P. indica. Further, Kd-PiPho85 -P. indica colonized plants were found to be susceptible to salt stress and showed less growth than that of wild type (WT) P. indica colonized plants. This suggests that PiPho85 plays a crucial role in conferring resistance to WT P. indica colonized plants during salt stress.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"322"},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The PGPB paradox: A critical review of field performance and practical constraints in agriculture. PGPB悖论：对农业领域表现和实际限制的批判性回顾。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-08 DOI: 10.1007/s11274-025-04552-y

Ismam Samonty, Md Zahidul Hasan, Md Rafiul Hossain, A G M Sofi Uddin Mahamud

{"title":"The PGPB paradox: A critical review of field performance and practical constraints in agriculture.","authors":"Ismam Samonty, Md Zahidul Hasan, Md Rafiul Hossain, A G M Sofi Uddin Mahamud","doi":"10.1007/s11274-025-04552-y","DOIUrl":"10.1007/s11274-025-04552-y","url":null,"abstract":"While PGPB have historically been applied in agriculture, their formal recognition in the last century has driven intensive research into their role as sustainable tools for improving crop yield and stress tolerance. As they are primarily sourced from wild or native environments, the widespread enthusiasm has led to heightened expectations surrounding their potential, often based on the assumption that biological solutions are inherently safer and more effective than synthetic inputs. However, despite their popularity, increasing reports of inconsistent or limited performance under real-world, field conditions have raised critical questions about their credibility as biofertilizers and biocontrol agents. This commentary critically evaluates the reasons behind the limited success of PGPB in field settings, based on the latest scientific evidence. Particular focus is placed on the microbial conflict in the rhizosphere, the inability of PGPB inoculants to adapt in complex environmental conditions, limitations in modern agricultural practices, formulation challenges, and regulatory gaps. The paper recommends the need for a paradigm shift in PGPB research and deployment strategies to better align laboratory successes with the challenges and outcomes seen in actual field conditions. Such an approach is vital for reducing over-reliance on underperforming technologies and promoting more resilient, context-specific solutions for sustainable agriculture, especially in under-resourced regions.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"321"},"PeriodicalIF":4.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent trends in microbial production of alkanes. 微生物生产烷烃的最新趋势。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-09-02 DOI: 10.1007/s11274-025-04536-y

Noura Sh A Hagaggi, Usama M Abdul-Raouf

{"title":"Recent trends in microbial production of alkanes.","authors":"Noura Sh A Hagaggi, Usama M Abdul-Raouf","doi":"10.1007/s11274-025-04536-y","DOIUrl":"10.1007/s11274-025-04536-y","url":null,"abstract":"Alkanes are saturated hydrocarbons characterized by their stable chemical properties and multiple uses in the energy sector. Although alkanes are found in nature in various sources like natural gas and petroleum, microbial alkane production has become a promising way to develop sustainable biofuels. Alkanes are produced by microorganisms such as bacteria, fungi, yeast, and algae via particular metabolic pathways. Although progress has been achieved, challenges remain, including low conversion efficiencies and excessive costs of biofuel production compared to conventional fossil fuels, along with the need to enhance microbial resistance to harsh production conditions. By understanding current progress and future challenges, researchers and bioengineers can develop modern technologies and methods to improve the efficiency and effectiveness of microbial alkane production. This review provides a detailed look at the recent developments in microbial biosynthesis of alkanes, highlighting the key metabolic pathways and genetic techniques used to scale up production. It also discusses the challenges and obstacles facing the production of microbial alkanes. The review emphasizes the role of microbial alkane production in promoting a sustainable energy future. This review may enhance awareness of the importance of developing renewable biofuel sources, which supports global efforts to combat climate change and achieve sustainable development.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"320"},"PeriodicalIF":4.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Escherichia coli biotransformation of biliverdin to synthesize bilirubin. 重组大肠杆菌生物转化胆绿素合成胆红素。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-26 DOI: 10.1007/s11274-025-04527-z

Yue Zhang, Haoni Luan, Wenhan Qiu, Wei Xu, Wei Feng, Fei Wang, Peng Song

引用次数: 0

Bacterial allies in chromium hyperaccumulation: native rhizobacterial dynamics of profusely growing Dactyloctenium aegyptium in highly tainted tannery sludge. 铬超积累中的细菌盟友：在高度污染的制革厂污泥中大量生长的埃及Dactyloctenium的原生根瘤菌动力学。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-26 DOI: 10.1007/s11274-025-04528-y

Pratishtha Sharma, Mohd Zobair Iqbal, Ram Chandra

{"title":"Bacterial allies in chromium hyperaccumulation: native rhizobacterial dynamics of profusely growing Dactyloctenium aegyptium in highly tainted tannery sludge.","authors":"Pratishtha Sharma, Mohd Zobair Iqbal, Ram Chandra","doi":"10.1007/s11274-025-04528-y","DOIUrl":"10.1007/s11274-025-04528-y","url":null,"abstract":"Tannery sludge has highly toxic heavy metals like chromium (Cr), posing environmental and health risks. This research investigates the potential of Dactyloctenium aegyptium (L.) Willd. and the associated rhizobacterial communities for bacterial-assisted phytoremediation of tannery sludge, having 6403.16 ± 0.71 mg/kg Cr. The analyses of culturable bacterial communities resulted in the exploration of two highly potent plant growth-promoting strains (CRB2 and CRB5), out of the six culturable strains obtained. SEM imaging depicted robust bacterial colonization on the plant root surface, confirming active plant-microbe interaction. D. aegyptium showed significant Cr accumulation (4936 ± 1.34 mg/kg) within the tissues, followed by substantial translocation to shoots and leaves (TF = 1.01). With a BCF of 1.66 for Cr, D. aegyptium bespeaks evident hyperaccumulation potential. TEM imaging revealed the granular metal deposition in the plant tissues. Post-plant growth, the sludge exhibited an 80.3% reduction in Cr concentration, alongside enhanced physicochemical properties (reduced pH, increased organic matter, reduced metal content). Furthermore, metagenomics analyses showed that the growth of D. aegyptium drastically changed rhizobacterial communities, decreasing species richness and increasing functional pathways associated with stress responses and metal tolerance. Important genes (copA, czcA, nirA), enzymes (dioxygenases, trimethylamine-N-oxide reductase), and proteins (CsgE, DsbG), essential for the nitrogen cycle, chromium detoxification, and plant-microbe associations, were found to be involved in metabolic pathways. The study amalgamates morphophysiological and advanced metagenomic approaches to put forth an understanding of species-specific plant-microbe interactions for the development of scalable and sustainable remediation and engineering of rhizospheric microbiomes for eco-restoration of heavy metal-polluted industrial sites.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"314"},"PeriodicalIF":4.2,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis of gamma rays-induced melanin-based bismuth oxide nanoparticles for evaluation of the antibacterial and anti-virulence activities against extra-intestinal pathogenic bacteria. 绿色合成伽马射线诱导的黑色素基氧化铋纳米颗粒，用于评估其对肠外致病菌的抗菌和抗毒活性。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-26 DOI: 10.1007/s11274-025-04533-1

Amira Y Mahfouz, Nermine N Abed, Amira S Abd-El-Aziz, Rasha Mohammad Fathy

引用次数: 0

Updates on therapeutic targeting of diguanylate cyclase for addressing bacterial infections: A comprehensive review. 二胍酸环化酶治疗细菌感染的最新进展：一项综合综述。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-26 DOI: 10.1007/s11274-025-04512-6

Roshan Mukund Tawale, Rafwana Ibrahim, Jesil Mathew Aranjani

{"title":"Updates on therapeutic targeting of diguanylate cyclase for addressing bacterial infections: A comprehensive review.","authors":"Roshan Mukund Tawale, Rafwana Ibrahim, Jesil Mathew Aranjani","doi":"10.1007/s11274-025-04512-6","DOIUrl":"10.1007/s11274-025-04512-6","url":null,"abstract":"The current global health issue of antimicrobial resistance necessitates innovative strategies for treating bacterial infections. A promising novel therapeutic target is the multisubunit diguanylate cyclase (DGC), which synthesizes cyclic di-GMP (c-di-GMP) and is implicated in biofilm formation. This multisubunit enzyme regulates critical virulence-associated behaviors in bacteria, such as biofilm formation, motility, and virulence factor synthesis, which are critical for biopathogenicity. This review focuses on the structural and functional characterization of DGCs, their contributions to bacterial pathogenesis, and recent advances in therapies targeting these enzymes. We describe innovations in small-molecule (SM) and peptide-based therapeutics and novel drug delivery platforms that alter DGC activity. In addition, we discuss new findings regarding DGCs and combination therapies of DGC inhibitors with other antibiotics. Finally, we outline the problems and prospects of therapies targeted to DGCs in the clinic. Inhibitors of DGCs may benefit from recent advances in structural biology techniques and medicinal chemistry approaches, which present new drug development opportunities.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"317"},"PeriodicalIF":4.2,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of environmental microplastics (MPs) degradation via bacteria under stress conditions: key enzymes, pathways, and mechanisms. 环境微塑料（MPs）在逆境条件下通过细菌降解的增强：关键酶，途径和机制。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-26 DOI: 10.1007/s11274-025-04525-1

Kanika Sharma, Monika Sharma, Nandini Thakur, Habib Ullah, Sedky H A Hassan, Yuanzhang Zheng, Xiangkai Li, Mohamed Sakran, El-Sayed S Salama

{"title":"Enhancement of environmental microplastics (MPs) degradation via bacteria under stress conditions: key enzymes, pathways, and mechanisms.","authors":"Kanika Sharma, Monika Sharma, Nandini Thakur, Habib Ullah, Sedky H A Hassan, Yuanzhang Zheng, Xiangkai Li, Mohamed Sakran, El-Sayed S Salama","doi":"10.1007/s11274-025-04525-1","DOIUrl":"10.1007/s11274-025-04525-1","url":null,"abstract":"Microplastics (MPs) are an emerging pollutant that needs effective bioremediation strategies. Strategies, including microbial implementation, enzymes, and insect-mediated degradation, have been effectively deployed and reviewed for the biodegradation of MPs. Thus, this review focused on utilizing multiple stressors (biotic and abiotic) to enhance MPs biodegradation. MPs degradation mechanism, major enzymes involved, and stress-mediated bacterial responses are highlighted. The key routes for MPs biodegradation under various stress are covered. Furthermore, the applications of stresses on wastewater treatment plants (WWTPs) for real-world application are also considered. Thermus sp. is reported to remediate polystyrene (PS) by 43.7% at 40-80 °C stress, whereas pH stress showed enhanced low-density polyethylene (LDPE) biodegradation (9.9%) under B. krulwichiae. Salinity up to 3 M NaCl, when applied to Bacillus sp., showed 48 times higher protease content. Radiation UV-C on P. aeruginosa increased polyethylene/polystyrene (PE/PS) protease activity by 75.47%. The bacterial response to stress was reported to be mediated by enzyme upregulation, biofilm formation, and metabolic shifts. Targeted stress enhanced MPs biodegradation through specific bacterial adaptations and enzymatic activity. Particular stress requires a specific mechanism to accelerate bacterial MPs degradation. Future research should aim to explore the synergistic effects of combined stressors, conduct comprehensive ecological risk assessments, and implement large-scale field trials to ensure the sustainability and ecosystem compatibility of stress-mediated MPs bioremediation.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 9","pages":"318"},"PeriodicalIF":4.2,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal nano-factories: synthesis and characterization of silver nanoparticles from soil-borne fungus and their anti-microbial potential. 真菌纳米工厂：土壤真菌中银纳米颗粒的合成和表征及其抑菌潜力。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-26 DOI: 10.1007/s11274-025-04530-4

Insha Amin, Ruqeya Nazir, Mushtaq Ahmad Rather

引用次数: 0

Developing a highly efficient whole-cell biotransformation of 3,4-dihydroxyacetophenone into Apocynin by engineered Escherichia coli expressing caffeic acid O-methyltransferase. 利用表达咖啡酸o -甲基转移酶的工程大肠杆菌将3,4-二羟基苯乙酮高效全细胞转化为罗布麻素。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-08-14 DOI: 10.1007/s11274-025-04526-0

Wenyu Wang, Xiwei Yuan, Yalun Zhang, Yanqiu Tian, Dehong Xu

{"title":"Developing a highly efficient whole-cell biotransformation of 3,4-dihydroxyacetophenone into Apocynin by engineered Escherichia coli expressing caffeic acid O-methyltransferase.","authors":"Wenyu Wang, Xiwei Yuan, Yalun Zhang, Yanqiu Tian, Dehong Xu","doi":"10.1007/s11274-025-04526-0","DOIUrl":"10.1007/s11274-025-04526-0","url":null,"abstract":"Apocynin (Ap), a bioactive compound from the roots of Picrorhiza kurroa, faces challenges in production. This study developed a whole-cell biotransformation approach using engineered Escherichia coli expressing caffeic acid O-methyltransferase to convert 3,4-dihydroxyacetophenone into Ap. Caffeic acid O-methyltransferase from Medicago sativa (MsCOMT) showed the highest activity, yielding 90.75 mg·L⁻¹ and 97.04 mg·L⁻¹ Ap after 24 h and 48 h, while the mutant I319A enhanced titers to 198.32 mg·L⁻¹ and 228.37 mg·L⁻¹ by optimizing H269-D270 catalytic mechanisms. Besides, this study explored the impact of biotransformation conditions on the activity of MsCOMT and the yield of Ap. TB medium was found to be the most effective, with yields of 158.52 mg·L⁻¹ and 174.75 mg·L⁻¹ after 24 h and 48 h. The in vivo SAM regeneration system, less effective than in vitro SAM supplementation, still improved Ap yield when the genes mtn, luxS, and MsCOMT were arranged in a pseudo-operon configuration. Orthogonal experiments showed the importance order of transformation factors as: transformation temperature > induction temperature > substrate concentration > IPTG concentration, with optimal conditions being 35 °C transformation temperature, 15 °C induction temperature, 8 mmol·L⁻¹ substrate concentration, and 0.1 mmol·L⁻¹ IPTG concentration. Using mutant MsCOMTI319A under these optimal conditions, Ap yield increased steadily with transformation time, reaching a maximum of 544 mg·L⁻¹ after 72 h. This research successfully achieved the whole-cell biotransformation of 3,4-dihydroxyacetophenone into Ap for the first time, providing a foundation for further optimization of Ap biosynthesis.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"313"},"PeriodicalIF":4.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0