World journal of microbiology & biotechnology最新文献_第6页

Genomic features of plant growth-promoting Ochrobactrum intermedium GH3, an endophyte associated with flatsedge (Cyperus sp.) native to Hochiminh City, Vietnam. 越南胡志明市原产于平莎草（Cyperus sp.）的促生长内生菌Ochrobactrum intermedium GH3的基因组特征

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-28 DOI: 10.1007/s11274-025-04502-8

Phuc Hoang Bui, Duy-Phuong Pham, Thi-Viet Nguyen, Thuy-Hien Nguyen, Nhat-Tan Le-Nguyen, Tam Dinh Le Vo, Trung-Dung Huynh-Ngoc, Truc-Thanh Tran, Thu Huynh

{"title":"Genomic features of plant growth-promoting Ochrobactrum intermedium GH3, an endophyte associated with flatsedge (Cyperus sp.) native to Hochiminh City, Vietnam.","authors":"Phuc Hoang Bui, Duy-Phuong Pham, Thi-Viet Nguyen, Thuy-Hien Nguyen, Nhat-Tan Le-Nguyen, Tam Dinh Le Vo, Trung-Dung Huynh-Ngoc, Truc-Thanh Tran, Thu Huynh","doi":"10.1007/s11274-025-04502-8","DOIUrl":"10.1007/s11274-025-04502-8","url":null,"abstract":"The study investigated the plant growth-promoting (PGP) characteristics of culturable endophytic bacteria associated with a rhizomatous perennial flatsedge (Cyperus sp.) native to Hochiminh City, Vietnam. Among the six bacteria isolated, strain GH3 demonstrated the highest potency in solubilizing phosphate and producing ammonia and IAA. Genomic analysis identified strain GH3 as Ochrobactrum intermedium, showing 97.87% ANI and 86.85% alignment to GTDB reference databases. Functional annotation revealed GH3's capabilities in secondary metabolism, iron acquisition, and osmotic stress tolerance. It exhibited potential phosphate solubilization (51.33 mg/L), ammonium (2.66 mg/L), siderophore (23.50%), and IAA (12.56 mg/L) production under stress conditions. The application of strain GH3 influenced growth in rice and tomato. In rice, SL were enhanced in DT8 (27%) and ST25 (54%). Leaf emergence was stable in rice DT8 and tomato, but increased by 28.2% in rice ST25. LRN was increased in rice DT8 (34.9%) and tomato (67.2%), with no change in rice ST25. PRL extended moderately in tomato (16.9%) and rice ST25 (15.2%), but not in rice DT8, while LRL decreased in rice DT8 (-10.9%) and ST25 (-31.4%). Moreover, improvement in biomass parameters emphasized GH3's potential in biomass enhancement with species-specific effects. This study highlights the promising PGP potential of endophytic O. intermedium GH3, which could aid sustainable agriculture amid climate change. It is the first investigation into O. intermedium isolated from Cyperus species, and the first report of O. intermedium in Vietnam, offering valuable contributions to our understanding of microbial interactions within Vietnam's unique flora.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"281"},"PeriodicalIF":4.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of irradiation insecticidal technology on microbiome, physicochemical properties of strong-flavor Baijiu Daqu. 辐照杀虫技术对烈性白酒大曲微生物群及理化性质的影响。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-28 DOI: 10.1007/s11274-025-04493-6

Yu Wang, Jiahao Xue, Yue Qiu, Xiaopeng Zhang, Dan Wang, Xiaoming Chen, Peng Gao, Liquan Du, Fei Xie, Xiaojun Xia, Qiang Liu, Yu Zeng

{"title":"Effect of irradiation insecticidal technology on microbiome, physicochemical properties of strong-flavor Baijiu Daqu.","authors":"Yu Wang, Jiahao Xue, Yue Qiu, Xiaopeng Zhang, Dan Wang, Xiaoming Chen, Peng Gao, Liquan Du, Fei Xie, Xiaojun Xia, Qiang Liu, Yu Zeng","doi":"10.1007/s11274-025-04493-6","DOIUrl":"10.1007/s11274-025-04493-6","url":null,"abstract":"The purpose of this study is to investigate the feasibility of utilizing high-energy electron beam irradiation for insecticidal purposes in Strong-flavor Baijiu Daqu (SBD) and to ascertain the irradiation threshold tolerable by SBD. According to the standard plate counting method, Illumina-MiSeq sequencing method and relevant industry testing standards, the effects of different irradiation doses (0-3.55 kGy) on the microbiome and Physicochemical properties of SBD were determined. Following irradiation, a reduction in the microbial population of SBD was observed, commensurate with the dose administered. However, subsequent to a 3-month storage period, SBD samples subjected to irradiation doses of 1.40 kGy or lower exhibited negligible disparities in physicochemical properties, microbial population, and microbial community structure when compared to non-irradiated counterparts. Conversely, irradiation doses exceeding 1.40 kGy resulted in marginal variations in physicochemical properties while notable discrepancies were noted in microbial abundance and community composition. Principal Coordinate Analysis (PCoA) revealed robust positive correlations between specific microbial taxa (e.g., Acetobacter and liquefaction, Saccharopolyspora and liquefaction, Streptomyces and saccharification, Thermoascus and acidity, Byssochlamys and moisture). Assessment of the efficacy of radiation doses ranging from 0 to 1.40 kGy in pest eradication indicated that a minimum irradiation dose of 0.27 kGy is imperative to prevent pest infestation during a storage duration of up to 120 days. In conclusion, this study proposes an optimal irradiation dosage range for insecticidal purposes in SBD, falling between 0.27 and 1.40 kGy. Additionally, it underscores the potential of high-energy electron beam irradiation as a viable method for quality assurance.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"279"},"PeriodicalIF":4.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-based prediction of nitrogen-fixing efficiency in Cowpea rhizobia from the Brazilian semiarid. 基于机器学习的巴西半干旱豇豆根瘤菌固氮效率预测。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-28 DOI: 10.1007/s11274-025-04491-8

Jardel da Silva Souza, Adriana Ferreira Martins, Flávio Pereira de Oliveira, Sandra Helena Unêda-Trevisoli

{"title":"Machine learning-based prediction of nitrogen-fixing efficiency in Cowpea rhizobia from the Brazilian semiarid.","authors":"Jardel da Silva Souza, Adriana Ferreira Martins, Flávio Pereira de Oliveira, Sandra Helena Unêda-Trevisoli","doi":"10.1007/s11274-025-04491-8","DOIUrl":"10.1007/s11274-025-04491-8","url":null,"abstract":"This study explores the potential of machine learning to predict nitrogen fixation efficiency in rhizobia strains associated with cowpea (Vigna unguiculata), aiming to optimize bioinoculant selection for sustainable agriculture. Eight native strains were isolated from soils in the Brejo Paraibano region (Brazil), characterized morphologically on Yeast Mannitol Agar YMA medium, and evaluated in greenhouse bioassays for nitrogen accumulation and Relative Index of Nitrogen Fixation Efficiency (IRF%). A Ridge Regression model was then developed using phenotypic colony traits as predictors to estimate Total Nitrogen and IRF%. The results demonstrated strong correlations between predicted and actual values (r = 0.95-0.96), suggesting that visible colony characteristics can serve as reliable proxies for strain efficiency. This approach has the potential to offer a cost-effective alternative to traditional greenhouse trials, with indications of reduced time and resource demands. However, these results are theoretical and require validation through larger datasets and field conditions before broad application in sustainable agriculture can be considered.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"274"},"PeriodicalIF":4.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biopriming with Endophytes improves plant resilience to develop climate-smart, futuristic agricultural crops. 利用内生菌进行生物灌溉可以提高植物的适应能力，从而开发气候智能型、未来的农作物。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-28 DOI: 10.1007/s11274-025-04485-6

Pragya Tiwari, Muhammad Adil, Kyeung-Il Park

{"title":"Biopriming with Endophytes improves plant resilience to develop climate-smart, futuristic agricultural crops.","authors":"Pragya Tiwari, Muhammad Adil, Kyeung-Il Park","doi":"10.1007/s11274-025-04485-6","DOIUrl":"10.1007/s11274-025-04485-6","url":null,"abstract":"In the face of climate adversities, the challenges faced by agricultural practices, depleting natural resources, rising hunger, and malnutrition necessitate harnessing bio-based resources, guided by the Sustainable Development Goals (SDGs) and transformative actions. For the development of sustainable food systems and climate-resilient crops, seed biopriming with beneficial microbes is gaining increased recognition, attributed to their favorable impact on plant growth, nutrient acquisition, and defense against pathogens. 'Seed primeomics' is a key concept in seed priming, and emphasizes the retention of stress memories, transgenerational microbial interactions, and plant stress resilience, as demonstrated by Trichoderma harzianum modulation of epigenetics in Lycopersicum esculentum roots. With an expanding scope, next-generation breakthroughs in nano-enabled seed biopriming can potentially address concerns associated with the performance and efficacy of the bio-inoculants in field conditions, as evident by ZnO nanopriming in fragrant rice, resulting in micro-nutrient fortified crops. Furthermore, encapsulation of microbial inoculants with nano-based polymers promotes plant development, while the nanoencapsulation procedure safeguards microbial strains and promotes shelf-life and controlled release, addressing key limitations with commercial applications. While the prospects of seed biopriming are immense and define novel avenues in sustainable agricultural practices, further research is essential to bridge the knowledge gaps. Herein, the article provides insights into how endophyte-mediated seed biopriming, as a potent and cost-effective green technology, is defining new avenues in ecosystem restoration, plant stress tolerance and adaptation, and the creation of climate-resilient crops capable of tolerating fluctuating climates, marking a new era in climate-smart agriculture.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"272"},"PeriodicalIF":4.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Lactobacillus delbrueckii subsp. bulgaricus VI104 as a D-lactic acid cell factory through strategic pathway optimization for enhanced biosynthesis. 德尔布鲁氏乳杆菌亚种的代谢工程。保加利亚菌VI104作为d -乳酸细胞工厂，通过战略性途径优化促进生物合成。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-28 DOI: 10.1007/s11274-025-04489-2

Payal Mukherjee, Senthilkumar Sivaprakasam

{"title":"Metabolic engineering of Lactobacillus delbrueckii subsp. bulgaricus VI104 as a D-lactic acid cell factory through strategic pathway optimization for enhanced biosynthesis.","authors":"Payal Mukherjee, Senthilkumar Sivaprakasam","doi":"10.1007/s11274-025-04489-2","DOIUrl":"10.1007/s11274-025-04489-2","url":null,"abstract":"Lactobacillus delbrueckii subsp. bulgaricus is a homofermentative lactic acid bacterium with recognized utility in dairy fermentation and unique capability to produce optically pure D-lactic acid (DLA), a critical monomer for polylactic acid-based bioplastics. Despite this potential, its industrial deployment has been constrained by poor galactose metabolism, incomplete lactose assimilation, and lack of efficient genetic tools. Here, we report the first comprehensive metabolic engineering of L. bulgaricus VI104 to establish a high-performance DLA cell factory. A tailored molecular toolbox was developed, including high-efficiency electroporation (~ 104 CFU µg⁻1 DNA), the pLEM415 shuttle vector, and strong constitutive promoters (PldhL), enabling stable heterologous gene expression. Galactose utilization was restored through heterologous expression of galK and galT, while ATP homeostasis was rebalanced via pyk overexpression. Further enhancement of glycolytic flux was achieved by co-expressing pfk, pgk, and the major dldh isoform. The engineered strain LdbVI104_07 exhibited a 3.4-fold improvement in acid tolerance and produced 9.39 g L⁻1 DLA with 99.09% optical purity and a yield of 0.188 g g⁻1 under optimized bioreactor conditions (3% lactose, 0.15% w/v supplemented glutamine and phenylalanine, 40 °C, pH 6.5). Multi-layer validation via transcriptomics revealed > 2.5-fold upregulation of core metabolic genes and downregulation of the global repressor ccpA, confirming metabolic rewiring. This work not only establishes L. bulgaricus as a genetically tractable and industrially relevant chassis but also provides a versatile platform for precision strain engineering in lactic acid bacteria, with direct applications in sustainable biomanufacturing, therapeutics, and synthetic biology.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"273"},"PeriodicalIF":4.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced catalytic stability for L-Dopa synthesis through cross-linking of Al₂O₃ nanocrystals with Bacillus subtilis tyrosine hydroxylase. Al₂O₃纳米晶体与枯草芽孢杆菌酪氨酸羟化酶交联增强左旋多巴合成的催化稳定性。

IF 4.2 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-28 DOI: 10.1007/s11274-025-04492-7

Areesha Batool, Sikander Ali, Aneeba Rashid, Muhammad Usman Ahmad, Saba Sana, Luiza C Campos

{"title":"Enhanced catalytic stability for L-Dopa synthesis through cross-linking of Al₂O₃ nanocrystals with Bacillus subtilis tyrosine hydroxylase.","authors":"Areesha Batool, Sikander Ali, Aneeba Rashid, Muhammad Usman Ahmad, Saba Sana, Luiza C Campos","doi":"10.1007/s11274-025-04492-7","DOIUrl":"10.1007/s11274-025-04492-7","url":null,"abstract":"This study focuses on enhancing L-Dopa synthesis by producing tyrosine hydroxylase from Bacillus subtilis SDSC-Env-i6 and its conjugation with aluminium oxide (Al₂O₃) nanoparticles. The free enzyme exhibited moderate activity, which was significantly improved through nanoparticle conjugation. Structural characterisation confirmed successful binding, with Al₂O₃ nanoparticles showing a crystalline nature and sizes ranging from 124 to 130 nm. Compared to the free enzyme, the Al₂O₃-conjugated tyrosine hydroxylase demonstrated markedly enhanced catalytic activity, reaching up to 15.3 ± 0.05 U/mL under optimised conditions. In contrast, maximum activities for the free enzyme were 5.5 ± 0.05 U/mL and 6.3 ± 0.11 U/mL in the presence of CuCl₂ and CaCl₂, respectively. The cross-linked enzyme also showed superior stability and efficiency across varying conditions. L-Dopa production was significantly higher with the conjugated enzyme, yielding between 0.531 and 1.105 mg/mL under optimised incubation conditions. These values were notably greater than those achieved using the free enzyme across different pH levels, volumes, and incubation times. Spectroscopic and microscopic analyses confirmed the integrity and functionality of the enzyme-nanoparticle complex. The observed improvements in both enzyme activity and L-Dopa yield were statistically significant (p ≤ 0.05), underscoring the potential of Al₂O₃ nanoparticle-conjugated tyrosine hydroxylase as an efficient and scalable biocatalyst for commercial L-Dopa production. Determination of kinetic parameters showed maximum Vmax/Km (1.25 × 106) was observed when immobilized tyrosinase activity was characterized with tyrosine as substrate, with significant Kcat value of 1.48 × 1018 s- 1.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"282"},"PeriodicalIF":4.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12304021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification and applications of glucose oxidase: optimization strategies and high-throughput screening technologies. 葡萄糖氧化酶的修饰与应用：优化策略与高通量筛选技术。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-12 DOI: 10.1007/s11274-025-04475-8

Zeyang Li, Yong Chen, Xihua Chen, Zhongjian Guo, Guoqiang Guan, Yong Feng, Huayou Chen

{"title":"Modification and applications of glucose oxidase: optimization strategies and high-throughput screening technologies.","authors":"Zeyang Li, Yong Chen, Xihua Chen, Zhongjian Guo, Guoqiang Guan, Yong Feng, Huayou Chen","doi":"10.1007/s11274-025-04475-8","DOIUrl":"10.1007/s11274-025-04475-8","url":null,"abstract":"Glucose oxidase (GOD), an oxidoreductase (EC 1.1.3.4), catalyzes the oxidation of β-D-glucose to gluconic acid using molecular oxygen as the electron acceptor, with concomitant generation of hydrogen peroxide. Owing to its versatile catalytic properties, GOD has garnered significant attention across diverse fields, including food and beverage manufacture, agriculture, biosensors and biotechnology. However, the inherent limitations of native enzymes, including susceptibility to inactivation under harsh conditions and insufficient catalytic efficiency, restrict their practical utility in advanced industry. This review systematically summarizes recent advances in molecular engineering strategies for GOD optimization, focusing on rational design and directed evolution approaches to improve its functional robustness and application adaptability in the bioeconomy. Furthermore, we highlight the prospective role of artificial intelligence (AI) and machine learning (ML) in addressing the classical activity-stability trade-off, enabling data-driven prediction of mutation hotspots and dynamic regulation of enzymatic properties. By integrating computational biology with experimental validation, this work proposes a theoretical framework and technical roadmap for developing \"tailored\" GOD variants that meet precise industrial requirements. The insights presented herein aim to bridge the gap between fundamental enzyme research and scalable biomanufacturing, fostering innovation in sustainable biotechnology.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"266"},"PeriodicalIF":4.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144620773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycelium-based products: multifunctional flexible mycomaterial, a textile alternative produced from fungi and lignocellulose waste. 基于菌丝体的产品：多功能柔性菌丝体材料，一种由真菌和木质纤维素废料生产的纺织品替代品。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-12 DOI: 10.1007/s11274-025-04470-z

Bruna Pertile, Roselei Claudete Fontana, Renan Isoton, Rosmary Nichele Brandalise, Marli Camassola

引用次数: 0

Harnessing microbial nanobiotics: Lactobacillus extracellular vesicles as next-generation therapeutics across physiological systems. 利用微生物纳米制剂：乳杆菌胞外囊泡作为跨生理系统的下一代治疗药物。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-11 DOI: 10.1007/s11274-025-04481-w

Ke Peng, Lina Liu, Shujun Gao

{"title":"Harnessing microbial nanobiotics: Lactobacillus extracellular vesicles as next-generation therapeutics across physiological systems.","authors":"Ke Peng, Lina Liu, Shujun Gao","doi":"10.1007/s11274-025-04481-w","DOIUrl":"10.1007/s11274-025-04481-w","url":null,"abstract":"Lactobacillus-derived extracellular vesicles (LEVs) are emerging as pivotal mediators of interkingdom communication, carrying strain-specific bioactive cargo that influences diverse physiological systems, including the gut, nervous, respiratory, reproductive, and integumentary systems. These nanoscale structures demonstrate significant therapeutic potential, modulating host immunity, enhancing barrier integrity, combating pathogens, and contributing to tissue repair across numerous health contexts. However, realizing their full clinical and biotechnological promise faces substantial hurdles. Key challenges include variability in isolation and purification methods, inconsistencies in LEV yield and composition due to cultivation conditions and inherent strain differences, and the observation of context-dependent or even paradoxical effects in certain diseases. Furthermore, gaps persist in understanding their precise biogenesis, cargo sorting mechanisms, and long-term biodistribution. Future progress necessitates interdisciplinary efforts to standardize production and characterization, resolve mechanistic ambiguities, and develop robust bioengineering strategies for targeted delivery and enhanced functionality. Addressing these obstacles is crucial to harness LEVs effectively as versatile tools in precision medicine, microbiome engineering, and sustainable applications in health and industry.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"261"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brucellosis in the omics era: integrative perspectives on Brucella genomic architecture, host-pathogen interactions, and disease dynamics. 组学时代的布鲁氏菌病：对布鲁氏菌基因组结构、宿主-病原体相互作用和疾病动力学的综合观点。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-07-11 DOI: 10.1007/s11274-025-04484-7

Shashi Bhushan Kumar, Giridhar Goudar, Manne Munikumar, Shanvanth R Arnipalli, Puneeta Singh Yaduvanshi, Virendra V Panpatil

{"title":"Brucellosis in the omics era: integrative perspectives on Brucella genomic architecture, host-pathogen interactions, and disease dynamics.","authors":"Shashi Bhushan Kumar, Giridhar Goudar, Manne Munikumar, Shanvanth R Arnipalli, Puneeta Singh Yaduvanshi, Virendra V Panpatil","doi":"10.1007/s11274-025-04484-7","DOIUrl":"10.1007/s11274-025-04484-7","url":null,"abstract":"Brucellosis is a widespread and persistent zoonotic disease caused by Gram-negative Brucella species, posing significant threats to human health, livestock, and economic stability. This review provides a comprehensive insight into current knowledge on Brucella pathogenicity, genomic structure and therapeutic challenges. We highlight the host-specific pathogenic mechanism of predominated species as B. melitensis, B. abortus, B. suis and B. canis focusing on their bipartite genome organization, virulence factors and strategies for intracellular survival and immune evasion. Diagnostic approaches including serological assays, such as SAT, ELISA, and molecular techniques like PCR molecular techniques are critically evaluated, alongside current antibiotic regimens and their limitations due to resistance and relapse. We further discuss promising developments in vaccine research-such as live attenuated, subunit, and DNA-based platforms-as well as adjunctive therapies involving nanotechnology, herbal compounds, and novel antibiotics. Emphasis is placed on the integration of genomic insights with therapeutic innovation. We conclude by identifying key research priorities, including the need for improved diagnostics, resistance profiling, and next-generation vaccines. This review underscores the importance of a multidisciplinary approach to mitigate the global burden of brucellosis and advance translational research in prevention and treatment.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"264"},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0