重组大肠杆菌生物转化胆绿素合成胆红素。

IF 4.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

World journal of microbiology & biotechnology Pub Date : 2025-08-26 DOI:10.1007/s11274-025-04527-z

Yue Zhang, Haoni Luan, Wenhan Qiu, Wei Xu, Wei Feng, Fei Wang, Peng Song

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引用次数: 0

摘要

胆红素是一种血红素衍生的代谢物，具有显著的生物活性，具有重要的制药应用潜力。本研究以大肠杆菌为基础构建了重组胆红素生物转化体系，通过异源表达褐家鼠一种新型胆红素还原酶（BvdR, XP_032760038.1）和巨斑拟虫一种葡萄糖脱氢酶（GDH），实现了胆红素的高效合成。在优化条件（520 mg/L胆绿素，pH 7.5, 37℃）下，系统的胆绿素转化率为99.2%，胆红素收率为89.9%。NADPH原位再生系统的集成显著降低了合成成本，为可扩展的胆红素生产提供了一种新的策略。

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Recombinant Escherichia coli biotransformation of biliverdin to synthesize bilirubin.

Bilirubin, a heme-derived metabolite with pronounced bioactivity, exhibits significant potential for pharmaceutical applications. This study developed a recombinant Escherichia coli-based biliverdin biotransformation system, enabling efficient bilirubin synthesis via heterologous expression of a novel biliverdin reductase (BvdR', XP_032760038.1) from Rattus norvegicus and a glucose dehydrogenase (GDH) from Priestia megaterium. Under optimized conditions (520 mg/L biliverdin, pH 7.5, 37 °C), the system achieved a biliverdin conversion rate of 99.2% and a bilirubin yield of 89.9%. The integration of an NADPH in situ regeneration system significantly reduced synthesis costs, providing a novel strategy for scalable bilirubin production.

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来源期刊

World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.30

自引率

2.40%

发文量

257

审稿时长

2.5 months

期刊介绍： World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.