World journal of microbiology & biotechnology最新文献

{"title":"Global landscape of Vibrio parahaemolyticus research: a bibliometric analysis.","authors":"Nor Hafizah Zakaria, Noorul Darlina Edlin Abd Rahim, Nur Fathiah Rosilan, Yeong Yik Sung, Khor Waiho, Sarahani Harun, Rabiatul Adawiah Zainal Abidin, Nor Afiqah-Aleng","doi":"10.1007/s11274-025-04262-5","DOIUrl":"10.1007/s11274-025-04262-5","url":null,"abstract":"<p><p>Vibrio parahaemolyticus poses a notable threat to marine ecosystems and can cause infections and disease outbreaks in seafood species, which can affect humans upon consumption. The global impacts of such infections and outbreaks on human and animal health led to a growing number of studies from various countries discussing the prevention, control, treatment, and overall implications of V. parahaemolyticus. Hence, this study aims to retrieve relevant studies on V. parahaemolyticus using a bibliometric analysis to understand current research status, trends, and hotspots regarding this bacteria. Relevant literature was searched across the Scopus database, and the data were subsequently analyzed using Biblioshiny software. In addition, a manual examination was conducted to identify the hosts of V. parahaemolyticus and diseases caused by the bacteria. Overall, 7,096 records were obtained from Scopus from 1963 to 2023. A bibliometric analysis identified 17,220 authors, with China emerging as the global leader. The analysis also highlighted significant keywords such as \"Vibrio parahaemolyticus,\" \"Litopenaeus vannamei,\" and \"innate immunity,\" suggesting a focus on the impact of V. parahaemolyticus on L. vannamei, specifically emphasizing the shrimp's innate immune responses. Host-disease interaction network also uncovered 53 interactions between hosts and diseases involving L. vannamei or Penaeus vannamei as the primary host, with acute hepatopancreas necrosis disease (AHPND) emerging as the most prevalent among them. This study can enhance our understanding of infections caused by V. parahaemolyticus and contribute to the development of effective strategies for their prevention and management.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"45"},"PeriodicalIF":4.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Colonization of Serendipita indica enhances resistance against Phoma arachidicola in Arachis hypogaea L.","authors":"Chen Wang, Taswar Ahsan, Ao Ding, Di Han, Jie Gao, Chun-Hao Liang, Si-Tong Du, Yi Wei, Yu-Qian Huang, Shi-Hong Zhang","doi":"10.1007/s11274-025-04263-4","DOIUrl":"10.1007/s11274-025-04263-4","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"46"},"PeriodicalIF":4.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable biodegradation of malachite green dye by novel non-pathogenic Pseudomonas aeruginosa ED24.","authors":"Akanksha Pandey, Sachin Kumar, Navneet Bithel, Sandeep Kumar, M Amin Mir","doi":"10.1007/s11274-025-04251-8","DOIUrl":"10.1007/s11274-025-04251-8","url":null,"abstract":"<p><p>Sustainable management of textile industrial wastewater is one of the severe challenges in the current regime. It has been reported that each year huge amount of textile industry discharge especially the dye released into the environment without pre-treatment that adversely affect the human health and plant productivity. In the present study, different bacterial isolates had been isolated from the industrial effluents and investigated for their bioremediation potential against the malachite green (MG) dye, a major pollutant of textile industries. The biochemical and molecular characterization of the bacterial strain showed the resemblance of most potent strain ED24 as Pseudomonas aeruginosa, which showed effective bioremediation potential against the MG dye. During response surface analysis (RSM), best MG degradation conditions have been observed at pH 7.0, 37 °C, 48 h, and 200 mg/L dye concentration, with highest degradation efficiency of 96.56 ± 0.8622 percent. Subsequently, supplementing various carbon and nitrogen sources increases MG decolorization by 1 to 2%, with beef extract (97.23%), sodium nitrate (97.46%), and maltose (98.67%). FT-IR results revealed the disappearance of distinct peaks, namely, 3328.275 cm^-1, 2102.842 cm^-1, 1101.140 cm^-1, and 559.04 cm^-1 from MG, and the formation of major intermediate compounds like leucomalachite green, benzoic acid, diacetamide, benzeneacetic acid, hexyl ester, ethyl 4-acetoxy butanoate, butanoic acid, and 2-methyl in GC-MS analysis of degraded dye sample confirms the biodegradation by bacterial strain ED24. The phytotoxicity studies on mung bean seeds confirmed MG dye toxicity reduction up to 67.53%, 54.16%, and 67.53% in biomass accumulation, root, and shoot lengths, respectively. Also, the microbial toxicity of MG was completely reduced on soil microflora Bacillus flexus, Stenotrophomonas maltophilia, Escherichia coli, Staphylococcus aureus, and Alternaria spp. The dual mitigation, both in microbial and plant systems, indicates the strong remediation potential of P. aeruginosa ED24 to break down MG dye ecologically sustainably.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"44"},"PeriodicalIF":4.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shifts of abundance and community composition of nitrifying microbes along the Changjiang Estuary to the East China Sea.","authors":"Yongkai Chang, Cheng Liu, Zongxiao Zhang, Dengzhou Gao","doi":"10.1007/s11274-025-04259-0","DOIUrl":"10.1007/s11274-025-04259-0","url":null,"abstract":"<p><p>Nitrification, the oxidation of ammonium to nitrate via nitrite, links nitrogen fixation and nitrogen loss processes, playing key roles in coastal nitrogen cycle. However, few studies have simultaneously examined both ammonia-oxidizing and nitrite-oxidizing microbes. This work investigated the abundance and community structure of ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB) using archaeal amoA gene, bacterial amoA gene, and NOB nxrB gene, respectively, through q-PCR and Sanger sequencing along the Changjiang Estuary salinity gradient. Results showed that ammonia oxidizers were dominated by AOB and had higher abundance than NOB. AOA had a higher diversity at high-salinity stations, and AOB diversity decreased along the estuarine salinity gradient. The communities of AOA differed among freshwater, estuarine mixing and seawater zones, indicating a narrow ecological niche. AOB compositions displayed a wide niche, changing from Nitrosomonas-like sequences dominated to Nitrosospira-like sequences dominated along the salinity gradient. The RDA showed that sand and nitrate contents had significant impacts on the AOA community compositions, while the AOB communities were governed by clay and nitrate contents. This research provides insight into the understanding of the niche of ammonia oxidizers in the estuarine zones.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"43"},"PeriodicalIF":4.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed bacterization with siderophore-producing bacteria: a strategy to enhance growth and alkaloid content in Catharanthus roseus.","authors":"Vyoma Mistry, Sapna Chandwani, Natarajan Amaresan, Deepti Kaushik, Ramar Krishnamurthy, Abhishek Sharma","doi":"10.1007/s11274-025-04257-2","DOIUrl":"10.1007/s11274-025-04257-2","url":null,"abstract":"<p><p>Catharanthus roseus is a medicinal plant widely known for producing monoterpenoid indole alkaloids (MIAs), including therapeutic compounds such as vinblastine and vincristine, which are crucial for cancer treatment. However, the naturally low concentration of these alkaloids in plant tissues poses a significant challenge for large-scale production. This study explores the application of siderophore-producing bacteria for seed bacterization of Catharanthus roseus to enhance the production of MIAs, including vindoline, catharanthine, and vinblastine. Utilizing High-Performance Liquid Chromatography (HPLC), we observed a significant increase in the concentration of these alkaloids in bacterized plants compared to controls. FTIR spectra of treated plants showed strong correlations with standard alkaloid mixtures, confirming higher alkaloid accumulation. Our findings demonstrate that bacterial siderophores play a vital role in optimizing iron uptake, which is crucial for secondary metabolite biosynthesis. This research highlights the potential of using microbial biotechnology to improve the yield of valuable pharmaceutical compounds in medicinal plants. Enhancing the biosynthetic pathways of MIAs offers a sustainable and efficient strategy for boosting the production of key therapeutic alkaloids in Catharanthus roseus, paving the way for advanced biotechnological applications in plant-based drug production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"42"},"PeriodicalIF":4.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications.","authors":"Rine Christopher Reuben, Carmen Torres","doi":"10.1007/s11274-024-04242-1","DOIUrl":"10.1007/s11274-024-04242-1","url":null,"abstract":"<p><p>Mammalian milk contains a variety of complex bioactive and nutritional components and microorganisms. These microorganisms have diverse compositions and functional roles that impact host health and disease pathophysiology, especially mastitis. The advent and use of high throughput omics technologies, including metagenomics, metatranscriptomics, metaproteomics, metametabolomics, as well as culturomics in milk microbiome studies suggest strong relationships between host phenotype and milk microbiome signatures in mastitis. While single omics studies have undoubtedly contributed to our current understanding of milk microbiome and mastitis, they often provide limited information, targeting only a single biological viewpoint which is insufficient to provide system-wide information necessary for elucidating the biological footprints and molecular mechanisms driving mastitis and milk microbiome dysbiosis. Therefore, integrating a multi-omics approach in milk microbiome research could generate new knowledge, improve the current understanding of the functional and structural signatures of the milk ecosystem, and provide insights for sustainable mastitis control and microbiome management.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"41"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioleaching of lanthanum from nickel metal hydride dry battery using siderophores produced by Pseudomonas sp.","authors":"Amany S Hegazy, Hoda M Soliman, Amr M Mowafy, Attiya H Mohamedin","doi":"10.1007/s11274-025-04250-9","DOIUrl":"10.1007/s11274-025-04250-9","url":null,"abstract":"<p><p>There is still much to be learned about the properties of siderophores and their applications. This study was designed to characterize and optimize the production of the siderophore produced by a marine bacterium Pseudomonas sp. strain ASA235 and then evaluate their use in bioleaching of rare earth elements (REEs) from spent Nickel-metal hydride (NiMH) batteries. The results of both Tetrazolium and Arnow's tests indicated that the test organism produces a mixed-type siderophore of pyoverdine family, a result that was confirmed by FT-IR and MALDI-TOFF analyses. Optimization of pH, temperature, incubation period, and iron concentration for siderophore production led to a noticeable shift from 44.5% up to 91% siderophore unit when the test bacterium was incubated at 28 °C and pH 7 after 72 h in the absence of iron. The purified siderophore showed the ability to bleach about 14.8% of lanthanum from the anode of the NiMH battery along with other elements, although in lower amounts. This data put siderophores in distinct focus for further prospective studies intending the bioleaching of such precious elements. The scaling up of this process and optimization would make a big difference in such a green bioleaching strategy, allowing us to recover such precious elements in an environmentally friendly way.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"39"},"PeriodicalIF":4.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the endomicrobiome of Podophyllum hexandrum to reveal the endophytic bacterial-association of in-planta podophyllotoxin biosynthesis.","authors":"Manju Kumari, Anish Tamang, Mohit Swarnkar, Pawan Kumar, Dinesh Kumar, Ashish Rambau Warghat, Vipin Hallan, Shiv Shanker Pandey","doi":"10.1007/s11274-024-04245-y","DOIUrl":"10.1007/s11274-024-04245-y","url":null,"abstract":"<p><p>Understanding the change in plant-associated microbial diversity and secondary metabolite biosynthesis in medicinal plants due to their cultivation in non-natural habitat (NNH) is important to maintain their therapeutic importance. Here, the bacterial endomicrobiome of Podophyllum hexandrum plants of natural habitat (NH; Kardang and Triloknath locations) and NNH (Palampur location) was identified and its association with the biosynthesis of podophyllotoxin (PTOX) was revealed. Rhizomes (source of PTOX) of plants of NH had highest endophytic bacterial diversity compared to NNH-plants. Presence of plant-location and tissue-specific distinct and common taxa were also identified. Acinetobacter, Ralstonia and Pseudomonas were identified as core taxa, present in plants of both NH and NNH. Predictive functional analysis of endophytic communities revealed abundant presence of genes encoding initial enzymes of PTOX biosynthesis and plant growth promotion in the rhizomes and roots of Kardang locations. Higher accumulations of secondary metabolites such as PTOX (2.78 and 2.11 folds in Kardang and Triloknath rhizomes, respectively; 1.48 and 1.71 fold in Kardang and Triloknath roots, respectively), Picropodophyllotoxin (3.08 fold in Kardang rhizomes), Quercetin (1.65 fold in Kardang and 1.32 fold in Triloknath rhizomes; 3.07-fold in Kardang and 1.60 fold in Triloknath roots) and Kaempferol (1.66 and 1.24-fold in Kardang and Triloknath rhizomes, respectively; 2.91 and 1.94-fold in Kardang and Triloknath roots, respectively) were also found in NH compared to NNH. This study provides novel insight into the change in the endomicrobiome of NH and NNH-plants and their correlation to secondary metabolites biosynthesis, and that must be considered for cultivation practices.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"38"},"PeriodicalIF":4.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the antibacterial efficacy of bee venom against multidrug-resistant pathogenic bacteria: Escherichia coli, Salmonella typhimurium, and Enterococcus faecalis.","authors":"Emad H El-Bilawy, Islam Mamdouh, Said Behiry, Islam I Teiba","doi":"10.1007/s11274-024-04248-9","DOIUrl":"10.1007/s11274-024-04248-9","url":null,"abstract":"<p><p>Bee venom (BV) represents a promising natural alternative to conventional antibiotics, particularly significant given its broad-spectrum antimicrobial activity and potential to address the growing challenge of antimicrobial resistance. The prevalence of antimicrobial-resistant microorganisms (AMR) is a global burden that affects human health and the economies of different countries. As a result, several scientific communities around the world are searching for safe alternatives to antibiotics. In this context, the present study represents a comprehensive investigation to evaluate the antibacterial effect of bee venom (BV) against Escherichia coli ATCC8739, Salmonella typhimurium ATCC14028, and Enterococcus faecalis ATCC25923. One mg of BV was extracted using 1 mL of DMSO to obtain a 1000 µg/mL solution. The chemical profile of the BV extract was determined using GC-MS, which revealed the presence of bioactive molecules with antimicrobial properties, such as astaxanthin, hycanthone, and fucoxanthin. The BV extract was tested against bacterial strains using different concentrations to obtain the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The results obtained revealed a high antibacterial activity of BV against the three strains with the highest MIC/MBC values of 12.5/25 µgml^- 1 against S. typhimurium. The antibacterial activity of the BV extract was compared to five conventional antibiotics using the disc diffusion method. The results showed a high antibacterial activity of the BV extract compared to different antibiotics with the largest inhibition zone obtained against E. faecalis at a value of 15 ± 0.22 mm compared to 9 ± 0.13 for azithromycin. The mode of action of BV, examined using scanning electron microscopy, proved a high effect of BV on the permeability of the bacterial plasma membrane. This study demonstrates bee venom's promising potential as a natural and eco-friendly antimicrobial agent, with activity against multiple bacterial strains, suggesting it may serve as an alternative to conventional antibiotics. The findings highlight the potential applications of BV in medical, agricultural, and veterinary fields, offering a sustainable solution to combat antimicrobial resistance. However, further studies are needed to fully assess its broad-spectrum antibacterial potential.Clinical trial number Not applicable.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"40"},"PeriodicalIF":4.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metabolomic analysis of Haematococcus pluvialis during hyperaccumulation of astaxanthin under the high salinity and nitrogen deficiency conditions.","authors":"Yong Dou, Liuyang Cheng, Yiwen Wang, Guihai Yu, Wenli Zhou","doi":"10.1007/s11274-025-04254-5","DOIUrl":"10.1007/s11274-025-04254-5","url":null,"abstract":"<p><p>Revealing the differences of metabolite profiles of H. pluvialis during hyperaccumulation of astaxanthin under the high salinity and nitrogen deficiency conditions was the key issues of the present study. To investigate the optimum NaCl and NaNO₃ concentration and the corresponding metabolic characteristic related to the astaxanthin accumulation in H. pluvialis, a batch culture experiment was conducted. The results indicated that 7.5 g·L^- 1 and 0 g·L^- 1 (nitrogen deficiency) were the optimum NaCl and NaNO₃ levels for the astaxanthin accumulation respectively, under which the highest astaxanthin contents reached up to 7.51mg·L^- 1 and 5.60mg·L^- 1. A total of 132 metabolites were identified using LC-MS/MS technique, among which 30 differential metabolites with statistical significance were highlighted. Subsequently, 18 and 10 differential metabolic pathways in the high salinity (HS) and nitrogen-deficient (ND) treatments were extracted and annotated respectively. The values of Fv/Fm, Yield and NPQ were all at the highest level in the ND group during the experiment. The levels of the metabolites in the ND group were almost lower than those both in the control (CK) and HS group, while which in the HS group were substantially at the higher or close levels compared to the CK group. Finally, 7 metabolic markers related to the astaxanthin accumulation were highlighted in the HS and ND group respectively. L-Proline, L-Aspartate, Uridine 5'-monophosphate (UMP), Succinate, L-2-Hydroxygluterate, L-Valine and Inosine 5'-monophosphate (IMP) were identified as the metabolic markers in the HS group, whose fold change were 0.85, 4.14, 0.31, 0.66, 3.10, 1.32 and 0.30. Otherwise, the metabolic markers were Glyceric acid, Thymine, sn-Glycerol 3-phosphate, Glycine, Allantoic acid, L-Valine and IMP in the ND group, with the fold change 0.23, 2.11, 0.38, 0.41, 0.50 and 2.96 respectively. The results provided the comparative metabolomic view of astaxanthin accumulation in H. pluvialis under the different cultivation conditions, moreover showed a novel insights into the astaxanthin production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"37"},"PeriodicalIF":4.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}