World journal of microbiology & biotechnology最新文献

{"title":"Functional divergence of bacterial communities in root zones of Suaeda salsa in an aged petroleum-polluted area of the Yellow River Delta.","authors":"Chang Zhou, Wenfang Wen, Lei Ji, Fanyong Song, Yingna Xing, Qi Li, Tianyuan Li, Xiaowen Fu","doi":"10.1007/s11274-026-05297-y","DOIUrl":"10.1007/s11274-026-05297-y","url":null,"abstract":"<p><p>Petroleum hydrocarbon pollutants pose a significant threat to the ecological security of oilfields. In these contaminated habitats, Suaeda salsa is a dominant halophyte of the coastal wetlands and oilfields, which has shown potential in remediation of petroleum polluted saline soils. However, the role of plant-associated microbial communities in pollutant attenuation remains poorly understood, particularly regarding niche-specific responses within the root zone. In this study, bacterial community structures associated with the halophyte Suaeda salsa in China's Yellow River Delta were investigated across three ecological niches comprising the endophytic tissues, rhizosphere soils, and bulk soils. A total of 54 samples from 6 sites were collected and analyzed using high-throughput absolute quantification sequencing and physicochemical analyses. Results demonstrated that petroleum hydrocarbons were the primary environmental drivers shaping bacterial community structure across all niches. Comparisons among ecological niches indicated that the rhizosphere effect further promoted the selection and enrichment of specific bacterial taxa, especially the genus Sphingomonas. Contrasting ecological strategies between niches were clearly resolved by co-occurrence network analysis, which showed highly connected networks in rhizosphere communities and modular structures in endophytic ones. Functional predictions further indicated niche-specific metabolic specialization, with endophytic communities enhancing xenobiotic degradation pathways and rhizosphere communities upregulating motility-related functions. These findings reveal the niche-dependent assembly and functional specialization of root-zone microbiomes under petroleum stress, thereby advancing our understanding of plant-microbe adaptive strategies in contaminated ecosystems.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in medium chain fatty acids biosynthesis from organic waste: current state, challenges and future prospects.","authors":"Yunhui Pu, Yang Luo, Dan Xu, Jialing Tang, Qingyuan Wang, Abdelfatah Abomohra","doi":"10.1007/s11274-026-05291-4","DOIUrl":"10.1007/s11274-026-05291-4","url":null,"abstract":"<p><p>Microbial conversion of organic waste into medium chain fatty acids (MCFAs) as the promising strategy to simultaneously achieve waste treatment and resource recovery has attracted increasing attentions in recent years. This review extensively summarized the organics conversion process, key influencing factors and emerging innovative strategies to enhance the MCFAs production. Moreover, the economic feasibility, challenges and research perspectives were also discussed. During the MCFAs production from organic waste, microorganisms involved in hydrolysis, acidogenesis and chain elongation processes coexist and collaborate to realize substrate conversion. Although competitive pathways such as methanogenesis, excessive ethanol oxidation and acrylate pathway disperse the carbon flow, adding chemical inhibitors, adjusting operation parameters and selecting suitable substrates can effectively suppress these competitive processes and promote the MCFA yield. Substrate properties, operational conditions and microbial community are critical factors influencing MCFAs biosynthesis. In addition, strategies such as exogenous additives, co-fermentation, electro-fermentation and microbial strengthening are feasible methods to boost MCFAs production. Furthermore, techno-economic assessment confirmed that MCFAs biosynthesis from organic waste is an economically feasible approach, but low MCFA yield, system instability and restriction of system scale-up are the main challenges for industrial application. This review provided useful information to carbon recovery from organic wastes and technique development.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in microbial production of D-aspartate.","authors":"Daiki Imanishi, Le Vi Pham, Shouji Takahashi","doi":"10.1007/s11274-026-05296-z","DOIUrl":"https://doi.org/10.1007/s11274-026-05296-z","url":null,"abstract":"<p><p>D-Aspartate (D-Asp) is a bioactive compound involved in neuroendocrine regulation and reproductive function in animals, including humans, with tissue-protective effects reported in experimental models, and has potential applications as a raw material for pharmaceuticals and functional food ingredients. It is also used as a side-chain precursor for semisynthetic antibiotics. An established industrial process for D-Asp uses enzymatic conversion by immobilized microbial cells with chemically synthesized D,L-Asp as the starting material and is constrained by dependence on chemical feedstock synthesis. Sustainable alternatives have therefore attracted attention: whole-cell bioconversion of exogenously supplied L-Asp has been demonstrated, whereas de novo production from a renewable carbon source has not yet been reported. This mini-review briefly notes the physiological and industrial contexts that motivate microbial production, and then focuses on the functions, biosynthesis, metabolism, and transport of D-Asp in microorganisms. We further discuss the screening of high-D-Asp-producing microorganisms using a high-throughput colorimetric assay based on D-aspartate oxidase, together with the molecular and cultivation characteristics of the strains identified. These studies indicate that racemization mediated by aspartate racemase, precursor supply via L-asparaginase, competing metabolism associated with bifunctional aspartate aminotransferase, and pH control during cultivation influence extracellular D-Asp accumulation; the contribution of membrane transport remains unresolved. Finally, we highlight the identification of D-Asp exporters, metabolic engineering of production strains, and optimization of cultivation processes as key future priorities for establishing a sustainable and reproducible fermentation platform for D-Asp production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13631041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomics of nitrogen acquisition and assimilation gene-family copy-number architecture across chlorophytes.","authors":"Prabhaharan Renganathan","doi":"10.1007/s11274-026-05304-2","DOIUrl":"10.1007/s11274-026-05304-2","url":null,"abstract":"<p><p>Nitrogen acquisition and assimilation determine nitrogen availability for algal growth and metabolism; however, their gene-family organization across chlorophytes remains poorly resolved. We examined 10 gene families (NiR, NR, NRT, AMT, GS, GOGAT, GDH, ASN, CPS-L, and CPS-S) in 30 chlorophyte genome assemblies using family-specific sequence-based curation. The assemblies represented 26 biological species after taxonomic standardization and pseudoreplication control. One representative assembly per species defined the primary dataset (D1; n = 26), with BUSCO C ≥ 80% (D2; n = 22) and C ≥ 90% (D3; n = 18) subsets used for genome-completeness sensitivity analyses. A BUSCO-based species phylogeny was used to visualize copy-number patterns relative to relationships among sampled taxa. AMT and NRT showed the broadest copy-number distributions, whereas NiR and the CPS subunits were predominantly single-copy. After normalization for the unequal number of families in each module, nitrogen acquisition had a higher copy-number per family than intracellular assimilation in D1 (Wilcoxon W = 8, p = 4.93 × 10^- 5), and this difference remained significant in D2 and D3. Of 45 pairwise gene-family associations in D1, none remained significant after Benjamini-Hochberg correction; isolated FDR-supported associations in D2 and D3 were not consistent across datasets. These results show heterogeneous nitrogen gene-family architecture across chlorophytes and indicate that the principal descriptive copy-number patterns were stable to genome-completeness filtering. The curated dataset, reproducibility package, and phylogenetic visualization framework provide a basis for further functional and gene-family evolutionary analyses.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial valorization of pomegranate peel pectin into pectic oligosaccharides with selective anticancer activities: in vitro and in silico evaluation.","authors":"Zahra Ghobadi, Afrouzossadat Hosseini-Abari, Hamed Amini-Rourani, Mohammad J Taherzadeh","doi":"10.1007/s11274-026-05298-x","DOIUrl":"10.1007/s11274-026-05298-x","url":null,"abstract":"<p><p>Pomegranate peel, a major by-product of processing, is rich in water-soluble pectin made of D-galacturonic acid units with α (1-4) linkage. Efficiently converting pomegranate peel into safe bioactive principles minimizes waste in the fruit industry and opens new avenues in functional foods. In this work, pomegranate pectin was enzymatically depolymerized with extracellular pectinases from Streptomyces hydrogenans YAM1 to generate pectic oligosaccharides (POS). Products were characterized by TLC and LC-ESI-MS, which indicated unsaturated mono-, di-, and trigalacturonic acids among the main species. Antioxidant activity was maximal at 5 mg mL⁻¹, as detected by the DPPH assay. The anticancer effects of the obtained POS were evaluated by assessing the viability of breast cancer (MCF-7), colorectal adenocarcinoma (Caco2), and neuroblastoma (SH-SY5Y) cells using MTT and flow cytometry assays. Cytotoxicity was also evaluated on normal cells (L929 and HUVEC). The results revealed that at 30 mg mL⁻¹, POS reduced the viability of MCF-7, Caco-2, and SH-SY5Y cancerous cells to < 20% while exhibiting no significant toxicity toward L929 and HUVEC normal cells. The observed efficacy against various cancer cells, without inducing cytotoxicity in healthy cells, distinguishes this compound from other anticancer agents. In the theoretical evaluation using the MM/PBSA method, the most suitable compounds were tetra-galacturonic acid and Δ4,5-unsaturated tri-galacturonate, whose binding free energies to the active site of the Gal-3 receptor were - 128.38 ± 21.51 kJ/mol and - 124.16 ± 23.16 kJ/mol, respectively. These findings support its potential as a unique nutritional compound for preventing and controlling cancer cell growth, advancing the development of next-generation functional pharmaceutical supplements.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring whole-genome analysis of a gamma-aminobutyric acid -producing marine Lactiplantibacillus plantarum: genomic insights into neuroactive and probiotic potential.","authors":"Eman H Zaghloul","doi":"10.1007/s11274-026-05275-4","DOIUrl":"10.1007/s11274-026-05275-4","url":null,"abstract":"<p><p>GABA-producing lactic acid bacteria are gaining attention as psychobiotics capable of influencing the microbiota-gut-brain axis, yet marine-derived strains remain poorly characterized. Here, the complete genomic characterization is reported for the marine strain Lactiplantibacillus plantarum EI6, focusing on its probiotic potential, GABA production, and safety. The 3.3 Mb genome (44.5% GC) encodes multiple probiotic-associated genes for acid/bile tolerance, adhesion, and stress response (including heat and cold-shock adaptation), antioxidant activity, and immunomodulation. Genome mining identified the glutamate decarboxylase system (gadB), and GABA production was experimentally confirmed by TLC and HPLC analysis, with a production level of 0.96 g/L. The genome also contains putative bacteriocin gene clusters (plantaricins and sactipeptides) and secondary metabolite BGCs (terpenes, RiPP-like, T3PKS). Safety analysis revealed no major virulence factors or antibiotic resistance genes, supporting a non-pathogenic profile. These findings position L. plantarum EI6 as a promising safe potential psychobiotic candidate for neuroactive and functional food applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quorum sensing molecules enhance cadmium detoxification and cellular removal in the marine diatom Phaeodactylum tricornutum.","authors":"Qingnan Yu, Siting Wang, Mengxuan Chang, Jiale Chen, Menglei Ye, Xinyu Pei, Yanping Wei, Chunhua Zhang, Ying Ge","doi":"10.1007/s11274-026-05294-1","DOIUrl":"10.1007/s11274-026-05294-1","url":null,"abstract":"<p><p>Acyl-homoserine lactones (AHLs) are ubiquitous bacteria-derived quorum-sensing molecules in marine environments and play important roles in regulating microbial physiological processes. However, their influence on metal detoxification and handling in microalgae remains poorly understood. Here, we investigated the effects of exogenous AHLs on cell growth, metabolic responses, and cadmium (Cd) removal in the marine diatom Phaeodactylum tricornutum under both Cd-free and Cd-contaminated conditions. It was demonstrated that AHLs slightly inhibited the growth of P. tricornutum and increased extracellular polymeric substances (EPS) production under Cd-free conditions. Under Cd exposure, AHLs significantly suppressed growth and enhanced oxidative stress responses, as indicated by increased activities of superoxide dismutase (SOD) and elevated levels of malondialdehyde (MDA) and glutathione (GSH). Besides, AHLs increased the Cd removal per cell of P. tricornutum, reaching 1.15-2.12 times that of the control. These results demonstrate that quorum sensing molecules can regulate detoxification-related metabolic responses and enhance cellular Cd handling in microalgae, providing new insights into microbe-mediated metal detoxification and highlighting the potential relevance of quorum sensing in microalgae-based biotechnological applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicle-like nanoparticles from Lactobacillus delbrueckii protect keratinocyte barrier function with involvement of TRPV4 signaling.","authors":"Chika Yamamoto, Satomi Uesugi, Daichi Ito, Rinka Kizaki, Nakako Katsuno, Juri Koizumi, Shigeo Takashima, Haruka Kashi, Hidetoshi Tachibana, Toshihide Tabata, Satoru Ogasahara, Hiroyuki Yamada, Ika Adhani Sholihah, Irmanida Batubara, Anggraini Barlian, Hiroshi Takemori","doi":"10.1007/s11274-026-05272-7","DOIUrl":"10.1007/s11274-026-05272-7","url":null,"abstract":"<p><p>Dry skin (xerosis) induced by low temperature and detergents may be alleviated by improving epidermal barrier function and reducing transepidermal water loss. Fermentation products from lactic acid bacteria can improve moisture retention, and extracellular vesicle-like nanoparticles (EV-LNPs) may contribute to this effect, although the mechanisms remain unclear. We investigated whether Lactobacillus delbrueckii-derived EV-LNPs protect keratinocyte barrier function and assessed the possible contributions of hydroxy fatty acids and transient receptor potential vanilloid 4 (TRPV4), a warmth-responsive Ca²⁺ channel. In PSVK1 human keratinocytes, low-temperature exposure reduced transepithelial electrical resistance (TEER) without affecting proliferation. EV-LNPs prepared from L. delbrueckii cultured in medium containing 10% low-fat milk restored TEER and increased zonula occludens-1 (ZO-1) and occludin expression. A lipophilic fraction concentrated 10-fold reproduced the TEER-enhancing effect of intact EV-LNPs. Mass spectrometry detected candidate 12-hydroxystearic acid and ricinoleic acid, which increased nuclear factor of activated T cells (NFAT) reporter activity and preserved junctional ZO-1 and occludin. Although hydroxy fatty acid levels in the active EV-LNP fraction were lower than the concentrations required for NFAT activation by the individual compounds, preincubation with EV-LNPs enhanced NFAT activation at lower 12-hydroxystearic acid concentrations. These findings suggest that EV-LNPs facilitate the activity of hydroxy fatty acids present at low abundance. Crotamiton attenuated NFAT reporter activation and barrier protection. Collectively, reporter, pharmacological, ectopic-expression, and lipid analyses support lipid-mediated TRPV4-associated signaling. L. delbrueckii-derived EV-LNPs and their lipophilic components may represent postbiotic factors for supporting keratinocyte barrier function under low-temperature and detergent stress.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of Glutamicibacter sp. ZY1 against Vibrio parahaemolyticus YDE17 pathogenicity in the clam Ruditapes philippinarum.","authors":"Yuanyuan Huang, Huili Hong, Tingting Li, Jian Zhang, Kai Liao, Jilin Xu, Weiwei Zhang","doi":"10.1007/s11274-026-05285-2","DOIUrl":"10.1007/s11274-026-05285-2","url":null,"abstract":"<p><p>The use of probiotics is preferable to the use of antibiotics for the control of vibriosis. In this study, artificial infection showed that Glutamicibacter sp. ZY1 significantly protected the Manila clam Ruditapes philippinarum from infection by Vibrio parahaemolyticus YDE17, with a relative percent survival of 82.16%. Glutamicibacter sp. ZY1 significantly reduced the total numbers of culturable Vibrio and V. parahaemolyticus in the infected clam R. philippinarum. Histopathological observation revealed that the pathological damage to the gills of individuals infected by V. parahaemolyticus YDE17 was alleviated by Glutamicibacter sp. ZY1. Furthermore, preinoculation with Glutamicibacter sp. ZY1 inhibited the biofilm formation of V. parahaemolyticus YDE17. Transcriptomic analysis revealed that V. parahaemolyticus YDE17 downregulated genes related to the immune response and peptide cross-linking in the clam R. philippinarum, and inhibited β-alanine metabolism and fatty acid degradation. Glutamicibacter sp. ZY1 activated the immune response, oxidative stress response, and heme/tetrapyrrole binding, and it upregulated pathways involved in arachidonic acid metabolism, arginine and proline metabolism, and the folate-mediated one-carbon pool. Glutamicibacter sp. ZY1 alleviated immune suppression and upregulated steroid hormone biosynthesis and linoleic acid metabolism. Taken together, Glutamicibacter sp. ZY1 shows promise as a probiotic candidate for protecting the clam R. philippinarum against Vibrio infection.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a bacterial mixed culture preventing bacterial storage diseases of root vegetables.","authors":"Anna Hoffmann, Weronika Zenelt, Krzysztof Krawczyk","doi":"10.1007/s11274-026-05279-0","DOIUrl":"10.1007/s11274-026-05279-0","url":null,"abstract":"<p><p>The storage of root vegetables is a critical aspect of agricultural production, significantly impacting both the quality and quantity of produce available for consumption. This study aimed to develop a bacterial consortium capable of preventing storage bacterial diseases in root vegetables. A total of 225 bacterial strains were isolated from various ecological niches, including crops, peri-root soils, coastal dune soils, sewage sludge, and symbiotic bacteria of common insect pests. These strains were characterized for their ability to grow at storage temperatures (4 °C) and produce cellulase, lipase, protease, and chitinase enzymes. From 225 isolates, 24 strains exhibited the highest enzymatic activities were selected for mutual inhibition assays, resulting in the selection of four strains (SP65, Dv004, Dv008, and Dv024a) that demonstrated high growth rates and enzymatic activities. The selected strains grew at 4 °C (OD₆₀₀ range: 0.061-0,190) and they exhibited the high intensity of production of the following enzymes measured as a halo diameter (cm) on bacterial medium: cellulase (from 1,4 to 1,8 cm), lipase (0,7) - 1,5), proteinase 1,2 - 1,6) and chitinase (0,3 - 0,4). These strains, identified as Stenotrophomonas maltophilia (SP65) and Serratia liquefaciens, were combined to form a bacterial consortium. The efficacy of this consortium was tested on potato (Solanum tuberosum L.), carrot (Daucus carota L.), celeriac (Apium graveolens L.), parsley (Petroselinum crispum Mill.), and onion (Allium cepa L.). The mixed culture was tested against bacterial soft rot pathogens including Pectobacterium carotovorum, Kosakonia cowanii, Enterobacter cloacae subsp. dissolvens and Burkholderia cepacia. For celeriac and parsley, the effect of inhibition of Pectobacterium carotovorum, Burkholderia cepacia, E. cloaceae subsp. dissolvens, Kosakonia cowanii, and the mixture of those pathogens were observed when pathogens were applied at concentration of 1,3 × 10⁸ CFU mL⁻¹. In case of onion the same effect of inhibition of growth of pathogens were observed only for P. carotovorum, and for B. cepacia at concentration of 1,3 × 10⁸ CFU mL⁻¹. The results demonstrated the potential of using a bacterial consortium as a biological control method to enhance the storage life and quality of root vegetables.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}