World journal of microbiology & biotechnology最新文献

{"title":"Biodegradation of phthalates DIBP, DMP, and DEP by Paenarthrobacter sp. strain PH1 - analysis of degradation, pathway, and its bioremediation potentiality in soil microcosm.","authors":"Moumita Mondal, Sujan Gayen, Subhankar Chatterjee","doi":"10.1007/s11274-025-04459-8","DOIUrl":"10.1007/s11274-025-04459-8","url":null,"abstract":"<p><p>Phthalic acid esters (PAEs), a class of anthropogenic hazardous chemicals, have become one of the lead toxic chemicals on the environmental contamination list. Their weak chemical bonding makes them easily assimilated into the environment, leading to serious environmental concerns. This study assessed the degradation of three low-molecular-weight phthalates, di-isobutyl phthalate (DIBP), diethyl phthalate (DEP), and dimethyl phthalate (DMP), in batch culture and artificial soil microcosms by a gram-positive strain Paenarthrobacter sp. PH1, isolated from a municipal waste dumping site in Chennai, Tamil Nadu, India. An intracellular esterase enzyme activity was found to be involved in phthalate degradation. Quantitative biodegradation analysis, conducted using high-performance liquid chromatography, depicted that the strain PH1 could degrade 1 g/L of DIBP, DEP, and DMP within 24 h, 48 h, and 60 h, respectively, in mineral media under batch shake culture conditions (28 °C, pH 7.0, rpm 180). When a mixture of three phthalates was used as a carbon source, all PAEs were completely degraded within 72 h. Besides that, artificial soil microcosm experiments exhibited that the strain PH1 could significantly degrade 63.38% and 66.89% of DIBP, 65.79% and 69.7% of DEP, and 64.91% and 69.07% of DMP within 12 days in sterile soil and unsterile soil, respectively. This is the first report on mixed phthalates degradation by any single strain in the soil microcosm. The findings highlight the potentiality of the strain PH1 towards the bioremediation of phthalate-contaminated soil, especially under mixed phthalate conditions, making it a potential candidate for multi-phthalate degradation in the soil environment.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"229"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-cultivation strategies for natural product discovery from actinomycetes: unlocking silent secondary metabolism with mycolic acid-containing bacteria.","authors":"Shumpei Asamizu","doi":"10.1007/s11274-025-04406-7","DOIUrl":"10.1007/s11274-025-04406-7","url":null,"abstract":"<p><p>Bacteria form consortia as integral components of diverse ecosystems, where they interact with various organisms. Within these communities, bacterial-bacterial communication plays a pivotal role by driving numerous specific interactions. A key aspect of this chemical communication is the production of secondary metabolites. Recent research demonstrates that interspecies interactions between microorganisms can serve as physiological triggers, activating silent biosynthetic gene clusters and leading to the synthesis of novel secondary metabolites by the interacting species. This review focuses on mixed cultivation strategies involving actinobacteria, with an emphasis on utilizing mycolic acid-containing bacteria such as Tsukamurella pulmonis as inducer organisms. It comprehensively examines recent advances striving to understand these bacterial interactions, specifically involving the ability of actinomycetes to recognize and respond to mycolic acid-containing bacteria to activate secondary metabolism. Furthermore, the genetic basis of secondary metabolism activation was explored and newly discovered secondary metabolites induced by actinobacteria-mycolic acid-containing bacteria co-culture were highlighted. Finally, the integration of combined-culture strategies with genetic engineering methods and the ecological relevance of actinobacteria-mycolic acid-containing bacteria interactions were discussed. These bacterial interactions provide an excellent model system for understanding the molecular mechanisms regulating secondary metabolism and could open new avenues for drug discovery.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"217"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses of microbial community to different depths in volcanic soils of Nvshan Volcano.","authors":"Jin Chen, Haining Wang, Ye Cui, Yiming Dai, Yueming Bao, Rui Qin, Qixiu Cheng, Xiaoyu Li","doi":"10.1007/s11274-025-04456-x","DOIUrl":"10.1007/s11274-025-04456-x","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"213"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production, downstream, stability, bioactivities, and applications studies of violacein and related compounds.","authors":"Simone Aparecida de Lima Scaramussa, Caroline Kie Ishimoto, Juliano Lemos Bicas","doi":"10.1007/s11274-025-04434-3","DOIUrl":"10.1007/s11274-025-04434-3","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"226"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical insight into gut microbial imbalance in Covid-19 and post vaccination heart attacks.","authors":"Mohamed Gamil Mehanna, Turky Omar Asar, Habib Changal Mudasir, Sherif Edris, Hadiah Bassam Al Mahdic, Ahmed F Bakry, Mustafa Zeyadi, Mirza Rafi Baig, Salma Naqvi, Fahad A Al-Abbasi, Omar A Al-Bar, Vikas Kumar, Firoz Anwar","doi":"10.1007/s11274-025-04431-6","DOIUrl":"10.1007/s11274-025-04431-6","url":null,"abstract":"<p><p>Gut dysbiosis (GD) influences myocardial infarction (MI), by promoting inflammation. Investigating compositional shifts in proinflammatory versus beneficial bacterial taxa may reveal novel biomarkers and therapeutic interventions. This study analysed stool samples from 30 patients who experienced myocardial infarction following COVID-19 infection or vaccination, and 20 healthy controls, using 16S rRNA sequencing to investigate gut microbial imbalances. OTUs were identified, and microbial differences were examined via PCA and PLS-DA to explore biomarkers and interventions. MI patients exhibited reduced Prevotella (17.19% vs. 30% in controls) and elevated Escherichia (16.11% vs.0. 28%), Salmonella (2.58% vs. 0.08%), and Bacteroides (15.91% vs. 8.18%), indicating proinflammatory Microbiota. At phylum level, Firmicutes increased (45.80% vs. 40.87%), Proteobacteria rose (13.27% vs. 9.91%), and beneficial Actinobacteria declined (2.78% vs. 3.67%). PCA showed distinct clustering, confirmed by Partial Least Squares Discriminant Analysis, highlighting heightened inflammation-driving taxa. Although Faecalibacterium appeared variably, it was overall diminished in MI patients, likely fueling immune activation. However, Bifidobacterium (2.06% vs. 2.57%) and Lactobacillus (0.63% v. 0.99%) decreased, compromising intestinal barrier integrity. Females had higher Fusobacterium (1.85% vs. ~ 0%) and Streptococcus (2.28% vs. 0.16%), whereas males exhibited increased Desulfovibrio (1.60% vs. 0.08%) and Akkermansia (1.02% vs. 0.04%). Finally, Enterobacteriaceae surged from 0.41% to 10.04%, exacerbating inflammatory cascades and underscoring dysbiosis in MI pathophysiology. Findings highlights pivotal role of dysbiosis in MI, with elevated proinflammatory bacteria (Escherichia, Salmonella, Proteobacteria) and reduced beneficial SCFA-producing taxa (Bifidobacterium, Lactobacillus) worsening inflammation. Sex-specific microbial alterations, characterized by an enrichment of Fusobacterium and Streptococcus in females and elevated levels of Desulfovibrio and Akkermansia in males, underscore their potential utility as clinical biomarkers for risk stratification. Targeted microbiota therapies can enhance cardiac care outcomes.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"206"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative microbiomes reveal microbial signatures in coffee fermentation and flavor development across distinct Brazilian biomes.","authors":"Natan Wiele, Gilberto Vinícius de Melo Pereira, Alexander da Silva Vale, Vitória de Mello Sampaio, Ana Isabel Ribeiro-Barros, Agnes de Freitas Diniz de Souza, Diogo Vinícius Nogueira Dos Santos, Aristóteles Góes-Neto, Carlos Ricardo Soccol","doi":"10.1007/s11274-025-04376-w","DOIUrl":"10.1007/s11274-025-04376-w","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"207"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Control of diarrhea and intestinal damage caused by enteropathogenic Escherichia coli using infant formula fermented with Lacticaseibacillus paracasei BMK2005: in vitro and in vivo evidences.","authors":"Kamel Bendjeddou, Amel Ait Meddour, Ahmed Adjebli, Roza Ourtirane, Djamila Sadoun, Djamel Drider","doi":"10.1007/s11274-025-04295-w","DOIUrl":"10.1007/s11274-025-04295-w","url":null,"abstract":"<p><p>Lacticaseibacillus paracasei BMK2005 (L. paracasei BMK2005) was isolated from the feces of a 1-year-old healthy Algerian infant. This strain, identified by 16S rDNA sequencing and API system, has demonstrated a remarkable antibacterial activity against enteropathogenic Escherichia coli in vitro and in vivo. In addition, this strain was shown to survive in a variety of environmental conditions that mimic the gastrointestinal tract conditions such as different pH values, bile salts and digestive enzymes. Moreover, L. paracasei BMK2005 was γ-hemolytic and sensitive to various antibiotics, supporting a safe characteristic for its application. Next, the anti-EPEC activity was then evaluated in an infant formula composed of 50% whey and 50% skim milk. A significant reduction in EPEC counts was observed after 24 h of incubation. These inhibitory properties were confirmed in an animal model using ten holoxenic rabbits made diarrheic by EPEC ingestion. L. paracasei BMK2005 was able to reduce the number of EPEC in the feces of diarrheic animals treated with the milk formula fermented by this strain. To support this study, histopathologic sections of the intestines of treated and untreated rabbits revealed clear injury in the case of animals infected with EPEC, an effect characterized by damaged villosities, while normal villosities were observed in samples from control or treated animals, delineating the protective effect of L. paracasei BMK2005.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"209"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the probiotic potential of Enterococcus spp.: Mechanisms and roles in animal and human health, A comprehensive review.","authors":"Donia Mechoub, Nacima Meguenni, Yacine Titouche, Ramzi Boubaker Elandoulsi, Sana Dhaouadi","doi":"10.1007/s11274-025-04389-5","DOIUrl":"10.1007/s11274-025-04389-5","url":null,"abstract":"<p><p>Enterococci, a subset of lactic acid bacteria, are ubiquitous in a variety of ecosystems, including the gastrointestinal tracts of mammalian and avian species. Due to their extreme adaptability, enterococci thrive in exceptionally unfavorable conditions characterized by high salinity levels, exposure to bile salts, a wide range of pH variations and high-temperature thresholds. Investigation into enterococcal strains as potential probiotics has encompassed the fields of human health, animal husbandry and companion animal welfare. In the context of human applications, the objectives are related to increasing immune responses, reducing cholesterol levels and providing anti-inflammatory and antioxidant attributes. Similarly, the livestock sector has recognized the potential of enterococci to stimulate growth, inhibit pathogen proliferation and amplify immune reactivity. Pet health interventions using enterococci aim to balance the microbiota, boost immune function and improve the animal's general well-being. The mechanisms of probiotic effects mediated by enterococci are manifold, including the production of enterocins, improvement of cholesterol levels, immunomodulation, antioxidant activities, attenuation of inflammatory cascades and the support of liver functions. While enterococcal probiotics hold great promises for optimizing human and animal health, safety considerations must be taken into account. Given the pathogenic character of enterococci and their capacity to carry antibiotic resistance determinants, rigorous evaluation of safety profiles is an imperative prelude to their judicious use as probiotics.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"214"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleic acid conjugated gold nanoparticles for biosensing applications: a review.","authors":"Tejaswini P Patil, Anuja A Vibhute, Ajayan Vinu, Arpita Pandey-Tiwari","doi":"10.1007/s11274-025-04454-z","DOIUrl":"10.1007/s11274-025-04454-z","url":null,"abstract":"<p><p>Gold nanoparticles (AuNPs) have gained widespread use in the development of biosensors for molecular diagnostics due to their ease of functionalization and distinctive optical properties, which are attributed to plasmonic effects. Functionalization strategies are widely applied to manipulate the properties of AuNPs which are then applied for various applications including drug delivery, biosensing, and bioimaging. Among the functionalised AuNPs, nucleic acid-conjugated gold nanoparticles (AuNPs-NA conjugate) have emerged as a powerful platform for biosensing applications due to their unique optical properties, biocompatibility, and ease of functionalization. This review provides an in-depth examination of the integration of nucleic acids with AuNPs for a wide range of biosensing technologies, including detection of pathogens and biomarkers. This review also discusses various methods of conjugating nucleic acids to gold nanoparticles, including covalent bonding, electrostatic interactions, and thiol-based linkages, while highlighting the key factors that influence the stability, specificity, and sensitivity of these conjugates. The paper further explores the optical, electrochemical, and fluorescent biosensing mechanisms enabled by AuNPs-NA conjugates. Finally, it provides a perspective on the future directions of AuNPs-NA conjugates in the development of rapid, cost-effective, and highly sensitive diagnostic tools for clinical and environmental monitoring.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"219"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosurfactant type and introduction order impact the morphology of biological CaCO₃ during the MICP process.","authors":"Weida Wang, Mingtao Zhu, Changxiong Zou, Tingting Zhang, Jianhong Han, Zhaoyu Wang","doi":"10.1007/s11274-025-04445-0","DOIUrl":"10.1007/s11274-025-04445-0","url":null,"abstract":"<p><p>In recent years, microbially induced carbonate precipitation (MICP) technology has attracted considerable attention; however, the morphology of biological calcium carbonate (Bio-CaCO₃) produced in MICP exhibites variability, which impacts its application. This study employed rhamnolipids and sophorolipids to investigate the impact of biosurfactant type and introduction order on the morphology of Bio-CaCO₃ in the MICP process. The findings indicated that the biosurfactant type and introduction order influenced the morphology of Bio-CaCO₃ and the composition of dissolved organic matter (DOM) in the solution. In the absence of biosurfactants, the Bio-CaCO₃ was primarily composed of rhombic calcite and spherical vaterite. The pre-introduction of rhamnolipids was observed to promote the formation of fasciculated agglomerates of calcite, while the pre-introduction of sophorolipids was observed to promote the formation of spherical agglomerates of calcite. The post-introduction rhamnolipids or sophorolipids were observed to stabilize hollow, spherical vaterite. The findings of the study indicated that the biosurfactant type and introduction order influenced the types and amount of DOM in solution, which in turn affected the MICP process and the morphology of Bio-CaCO₃. This study provides a theoretical foundation for the regulation of the morphology of Bio-CaCO₃ and the application of MICP technology.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"205"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}