World journal of microbiology & biotechnology最新文献

{"title":"Genetically engineering central carbon and nitrogen metabolism in Bacillus paralicheniformis for high γ-PGA production via glutamate-independent pathway.","authors":"Jishuai Qi, Rui Wang, Zirui Zhan, Jianghan Wang, Heli Shi, Xiuyun Zhao, Jun Tan, Gaofu Qi","doi":"10.1007/s11274-025-04467-8","DOIUrl":"10.1007/s11274-025-04467-8","url":null,"abstract":"<p><p>Poly-γ-glutamic acid (γ-PGA) is a biopolymer with great significance and broad applications. However, its fermentation in Bacillus strains usually requires costly glutamate supplementation. To reduce production costs, we engineered B. paralicheniformis to efficiently produce γ-PGA in a glutamate-independent manner by enhancing de novo glutamate biosynthesis via rocG-mediated pathways and alleviating carbon/nitrogen catabolite repression. In this study, we deleted three key regulatory genes: ccpA (catabolite control protein A), cggR (central glycolytic genes repressor), and tnrA (master regulator of nitrogen assimilation) to relieve repression on the TCA cycle, glycolysis, and γ-PGA biosynthesis pathways, thereby significantly increasing γ-PGA yield and productivity in B. paralicheniformis. In batch fermentation experiments, strains ΔccpA and ΔtnrA achieved γ-PGA yields of 63.86 g/L and 61.38 g/L, with productivities of 19.50 g/(L·h) and 6.93 g/(L·h) during the rapid increase period of γ-PGA production, respectively. To our knowledge, the ΔccpA strain achieved the highest γ-PGA yield and productivity among glutamate-independent producers. Both ΔccpA and ΔtnrA strains demonstrated superior efficiency in converting inexpensive NaNO₃ into high-value γ-PGA compared to the parental strain. Collectively, relieving suppression of carbon metabolism (the TCA cycle and glycolysis) and nitrogen metabolism (nitrate reduction) via genetic engineering holds significant potential for further enhancing the ability to biosynthesize γ-PGA in a glutamate-independent manner. The findings highlight the effectiveness of targeted genetic modifications in improving industrial bioprocesses for cost-efficient γ-PGA production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"236"},"PeriodicalIF":4.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunization of mice and goats with Corynebacterium pseudotuberculosis-derived rPTS, rRBN and rCP40 recombinant proteins.","authors":"Ioná Brito de Jesus, Daniela Droppa-Almeida, Caroline Ferreira, Roberto José Meyer Nascimento, Ricardo Wagner Portela, Mara Thais de Oliveira Silva, Mirna Samara Dié Alves, Guilherme Senna Dos Santos, Vasco Azevedo, Francine Ferreira Padilha, Sibele Borsuk","doi":"10.1007/s11274-025-04411-w","DOIUrl":"10.1007/s11274-025-04411-w","url":null,"abstract":"<p><p>Caseous lymphadenitis (CLA) is a chronic disease that affects sheep and goats worldwide and causes significant economic losses, and the best strategy to reduce clinical cases is vaccination. This study evaluated the immune response and protection rates of the Corynebacterium pseudotuberculosis-derived ascorbate transporter subunit (rPTS) and Ribonuclease protein (rRBN) recombinant proteins in mice and goats. Groups of eight Swiss mice each were inoculated with rPTS + Al(OH)₃ (G1), rPTS + Saponin (G2), rRBN + Al(OH)₃ (G3), rRBN + Saponin (G4), rPTS + rRBN + rCP40 + Al(OH)₃ (G5), rPTS + rRBN + rCP40 + Saponin (G6), Control Al(OH)₃ (G7) and Control Saponin (G8), respectively. The mice received two vaccine doses and were challenged with a virulent C. pseudotuberculosis strain. In addition, five groups of eight goats each were inoculated with sterile saline (G1), rPTS (G2), rRBN (G3), rCP40 (G4), and rPTS + rRBN + CP40 (G5), associated with saponin. ELISAs was used to detect specific total IgG in mice and goats, and the goat specific IFN-γ production was quantified. All immunized mice presented a peak of specific total IgG on day 60, and a higher survival rate (75%) was achieved in animals immunized with a pool of the recombinant proteins associated with saponin. Also, goats immunized with the same formulation produced significant levels of specific antibodies from day 15 post immunization and were able to significantly produce specific IFN-γ at 90 days post-immunization. Our findings suggest that the formulation containing the association of the proteins rPTS, rRBN and rCP40 associated to saponin adjuvant resulted in the best protection against the challenge in mice and was able to elicit an IFN-γ-characterized Th1 immune response in goats, and can be considered as a promising vaccine formulation for CLA.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"234"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quorum quenching of phenolic compounds from Leptolyngbya spp. MACC 32 via downregulation of the periplasmic receptor LuxP in Vibrio harveyi: Hinting a putative mechanism.","authors":"Madiyan Saranya, Byeol Ryu, Jose Seena, Bhaskaran Silpa, Ameer Ahna, C Benjamin Naman, Evgenia Glukhov, T P Sajeevan, I S Bright Singh, William H Gerwick, Joseph Valsamma","doi":"10.1007/s11274-025-04378-8","DOIUrl":"10.1007/s11274-025-04378-8","url":null,"abstract":"<p><p>Vibrio harveyi causes vibriosis, leading to high mortality and economic loss in global aquaculture. Quorum sensing (QS) driven biofilm formation makes them more resistant to various control measures. This study examined QS inhibition (QSI) of V. harveyi LB3 using marine cyanobacterial natural products. Qualitative and quantitative (88%) assays confirmed the quorum quenching (QQ) efficiency of the intracellular component of Leptolyngbya spp. MACC 32. The active fraction (E3) of the extract showed less than 50% toxicity to PmLyo-Sf9 cells and brine shrimp at 1 mg mL^-1. At MIC (0.06 mg mL^-1), E3 reduced biofilm biomass by 40-45% and metabolic activity by 10-20% without affecting bacterial growth. V. harveyi LB3 treated with E3 showed less gelatinase (64.75%), hemolytic activity (17.55%), motility (8-16%) and reduced EPS formation (45.5%), indicating its anti-virulence properties. HPLC, ¹H NMR, and GC-MS analyses confirmed that E3 contains 4-hydroxybenzoic acid (4-HBA) and 4-hydroxybenzaldehyde (4-HBAL). Synthetic controls validated the QQ of 4-HBA and 4-HBAL. Molecular docking studies hinted at the putative interaction of 4-HBA and 4-HBAL with QS proteins (LuxR, LuxP, LuxM, LuxN, LuxQ, LuxU, LuxO and LuxS) of V. harveyi. Gene expression studies showed strong LuxR, LuxP, LuxQ, and ToxR downregulation (61-97%), differential upregulation of LuxO, LuxU, aphA, qrr1-qrr5 (9-18%), and LuxS, LuxM, LuxN, Fur (2-4%). Although not confirmed with mutant strains, this study provides strong evidence that phenolic compounds from Leptolyngbya spp. MACC 32 inhibited V. harveyi LB3 QS by blocking AI-2 binding to LuxP, supporting their role in shrimp health management through the control of vibriosis.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"233"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in microbial production and consumption of nitrous oxide in agricultural soils.","authors":"Ruonan Xiong, Nan Gao, Weiqiang Huang, Xiaoyue Zhang, Weishou Shen","doi":"10.1007/s11274-025-04464-x","DOIUrl":"10.1007/s11274-025-04464-x","url":null,"abstract":"<p><p>Agricultural soil is a significant source of nitrous oxide (N₂O), a long-lived greenhouse gas. Several microbial processes in the nitrogen cycle generate N₂O but the only known sink for N₂O in the biosphere is the reduction of N₂O to N₂ catalyzed by N₂O reductase (NosZ). In this review, we summarized the latest knowledge on (i) key microbial pathways regulating N₂O production and consumption processes in agricultural soils, including nitrification and denitrification and (ii) emerging strategies for microbial-mediated mitigation of N₂O emissions from agricultural soils, including the use of nitrification and denitrification inhibitors, and the direct use of microorganisms to enhance NosZ activity. We focused on the screening and application strategies for microorganisms that can mitigate N₂O emissions. We summarized two ecological mechanisms of microbial inoculation mitigation of N₂O emissions from agricultural soils. One mechanism involved employing N₂O-reducing microorganisms containing nosZ gene to directly mitigate N₂O emissions from agricultural soils. The other mechanism utilized plant growth-promoting rhizobacteria to alter the community composition, abundance and activity of the N₂O-producing or -reducing microorganisms and indirectly mitigate N₂O emissions from agricultural soils. Additionally, we discussed the potential challenges affecting microbial inoculation technology, and explored its application prospects for reducing N₂O emissions from agricultural soils. By providing a comprehensive overview of these topics, we aimed to effectively design and apply microbial-mediated mitigation technologies to better manage and mitigate N₂O emissions from agricultural soils, ultimately contributing to global climate change mitigation efforts.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"235"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized solid lipid nanoparticles for co-delivery of gentamicin and doxycycline: a novel approach to combat intracellular brucella abortus infections.","authors":"Bandar Almutairy, Sitah Alharthi, Hasan Ebrahimi Shahmabadi, Seyed Ebrahim Alavi","doi":"10.1007/s11274-025-04427-2","DOIUrl":"10.1007/s11274-025-04427-2","url":null,"abstract":"<p><p>This study evaluates the potential of gentamicin (GEN) and doxycycline (DOX) co-loaded solid lipid nanoparticles (SLNs) for treating Brucella abortus infections. Optimized SLN formulations demonstrated mean particle sizes of 211.2 ± 10.8 nm, a polydispersity index of 0.42 ± 0.019, encapsulation efficiency of 95.3 ± 1.2%, and loading efficiency of 14.7 ± 0.1%. Drug release profiles revealed a biphasic release, achieving 91.1% and 73% cumulative release for GEN and DOX, respectively, over 72 h. In vitro studies on J774.A1 macrophages indicated significantly enhanced antibacterial activity and reduced cytotoxicity for SLN-GEN/DOX, achieving a minimum inhibitory concentration of 1.25 µg/mL and reducing intracellular bacterial load to 3.7 ± 0.02 Log₁₀ colony forming unit (CFU). In vivo, SLN-GEN/DOX-treated mice exhibited superior bacterial clearance, with spleen bacterial counts of 1.4 ± 0.03 Log₁₀ CFU compared to 2.4 ± 0.06 Log₁₀ CFU for the free drug combination. Additionally, reduced systemic toxicity was observed, with ALT and AST levels of 42.3 ± 1.2 U/mL and 64.1 ± 1.6 U/mL, respectively. These findings suggest that SLN-GEN/DOX offers a promising therapeutic approach for brucellosis by improving drug stability, bioavailability, and efficacy while minimizing systemic toxicity. Further studies should focus on clinical applications and mechanistic insights into SLN-mediated drug delivery.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"232"},"PeriodicalIF":4.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multifaceted approach of Rhizobium sp. PS1 for biodegradation of azo dye blue GSL and plant growth promotion by alleviating dye induced stress.","authors":"Mohini Pimpalse, Naga Rathna Supriya G","doi":"10.1007/s11274-025-04443-2","DOIUrl":"10.1007/s11274-025-04443-2","url":null,"abstract":"<p><p>Current research has focused on bacteria which have bioremediation ability of textile dye along with plant growth promoting activities. Based on decolorization potential done by plate assay and broth assay, one isolate has the highest decolorization efficiency (91.56%) against Blue GSL azo dye (100 ppm) and identified as Rhizobium sp. PS1 (OP303241). Based on UV-Vis spectral analysis, bacterial treated dye showed a gradual decrease peak compared to untreated. The efficacy of biodegradation was confirmed by analytical methods such as TLC, FTIR, and HPLC, which confirmed the reduction of the azo bond. Rhizobium sp. PS1 exhibited multiple promote plant growth traits such as the nitrogen-fixing ability, solubilize phosphate, produce IAA, siderophores and gibberellin. The phytotoxicity and microtoxicity of the dye degraded products showed reduced toxicity compared to the control dye. Rhizobium sp. PS1 revealed the advantageous effects of on Vigna radiata L. (GM-6) with increased germination rates, physical parameters, and chlorophyll content under dye stress. Analysis of the antioxidant enzyme demonstrated the ability of the strain to mitigate the dye-induced oxidative stress, while the significant reduction of dye concentration in the soil highlights the remediation effectiveness. Overall, this study illustrates the dual capability of Rhizobium sp. PS1 for azo dye degradation and plant growth promotion, offering an eco-friendly approach to improve productivity in dye-contaminated land.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"210"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of phthalates DIBP, DMP, and DEP by Paenarthrobacter sp. strain PH1 - analysis of degradation, pathway, and its bioremediation potentiality in soil microcosm.","authors":"Moumita Mondal, Sujan Gayen, Subhankar Chatterjee","doi":"10.1007/s11274-025-04459-8","DOIUrl":"10.1007/s11274-025-04459-8","url":null,"abstract":"<p><p>Phthalic acid esters (PAEs), a class of anthropogenic hazardous chemicals, have become one of the lead toxic chemicals on the environmental contamination list. Their weak chemical bonding makes them easily assimilated into the environment, leading to serious environmental concerns. This study assessed the degradation of three low-molecular-weight phthalates, di-isobutyl phthalate (DIBP), diethyl phthalate (DEP), and dimethyl phthalate (DMP), in batch culture and artificial soil microcosms by a gram-positive strain Paenarthrobacter sp. PH1, isolated from a municipal waste dumping site in Chennai, Tamil Nadu, India. An intracellular esterase enzyme activity was found to be involved in phthalate degradation. Quantitative biodegradation analysis, conducted using high-performance liquid chromatography, depicted that the strain PH1 could degrade 1 g/L of DIBP, DEP, and DMP within 24 h, 48 h, and 60 h, respectively, in mineral media under batch shake culture conditions (28 °C, pH 7.0, rpm 180). When a mixture of three phthalates was used as a carbon source, all PAEs were completely degraded within 72 h. Besides that, artificial soil microcosm experiments exhibited that the strain PH1 could significantly degrade 63.38% and 66.89% of DIBP, 65.79% and 69.7% of DEP, and 64.91% and 69.07% of DMP within 12 days in sterile soil and unsterile soil, respectively. This is the first report on mixed phthalates degradation by any single strain in the soil microcosm. The findings highlight the potentiality of the strain PH1 towards the bioremediation of phthalate-contaminated soil, especially under mixed phthalate conditions, making it a potential candidate for multi-phthalate degradation in the soil environment.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"229"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-cultivation strategies for natural product discovery from actinomycetes: unlocking silent secondary metabolism with mycolic acid-containing bacteria.","authors":"Shumpei Asamizu","doi":"10.1007/s11274-025-04406-7","DOIUrl":"10.1007/s11274-025-04406-7","url":null,"abstract":"<p><p>Bacteria form consortia as integral components of diverse ecosystems, where they interact with various organisms. Within these communities, bacterial-bacterial communication plays a pivotal role by driving numerous specific interactions. A key aspect of this chemical communication is the production of secondary metabolites. Recent research demonstrates that interspecies interactions between microorganisms can serve as physiological triggers, activating silent biosynthetic gene clusters and leading to the synthesis of novel secondary metabolites by the interacting species. This review focuses on mixed cultivation strategies involving actinobacteria, with an emphasis on utilizing mycolic acid-containing bacteria such as Tsukamurella pulmonis as inducer organisms. It comprehensively examines recent advances striving to understand these bacterial interactions, specifically involving the ability of actinomycetes to recognize and respond to mycolic acid-containing bacteria to activate secondary metabolism. Furthermore, the genetic basis of secondary metabolism activation was explored and newly discovered secondary metabolites induced by actinobacteria-mycolic acid-containing bacteria co-culture were highlighted. Finally, the integration of combined-culture strategies with genetic engineering methods and the ecological relevance of actinobacteria-mycolic acid-containing bacteria interactions were discussed. These bacterial interactions provide an excellent model system for understanding the molecular mechanisms regulating secondary metabolism and could open new avenues for drug discovery.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"217"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses of microbial community to different depths in volcanic soils of Nvshan Volcano.","authors":"Jin Chen, Haining Wang, Ye Cui, Yiming Dai, Yueming Bao, Rui Qin, Qixiu Cheng, Xiaoyu Li","doi":"10.1007/s11274-025-04456-x","DOIUrl":"10.1007/s11274-025-04456-x","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"213"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production, downstream, stability, bioactivities, and applications studies of violacein and related compounds.","authors":"Simone Aparecida de Lima Scaramussa, Caroline Kie Ishimoto, Juliano Lemos Bicas","doi":"10.1007/s11274-025-04434-3","DOIUrl":"10.1007/s11274-025-04434-3","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"226"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}