Harnessing Pseudodesulfovibrio sp., a sulfate-reducing bacterium for the sustainable synthesis of cerium sulfide.

IF 4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sonal Shete, Neelam Kapse, Prashant K Dhakephalkar
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引用次数: 0

Abstract

The present study investigates a novel sulfate-reducing bacterium, Pseudodesulfovibrio sp. MCM B-508, isolated from produced water, for its ability to produce cerium sulfide, an industrially valuable rare earth colorant. The study demonstrates a microbial route for pigment synthesis by cloning and expressing dissimilatory sulfite reductase (dsrAB) genes from this bacterium in Escherichia coli. The enzyme dissimilatory sulfite reductase shared a maximum of 94.09% homology with published dissimilatory sulfite reductase sequences from the GenBank database, highlighting its novelty. Using molecular engineering techniques, including chaperone co-expression with the pGro7 vector, the sulfate conversion efficiency was significantly enhanced to 71.23%, overcoming limitations in sulfate-reducing bacterial processes. The research successfully converted cerium sulfate to the desired gamma-form cerium sulfide (Ce2S3) through a recombinant E. coli strain, achieving a more efficient and eco-friendly production method compared to conventional chemical synthesis approaches. X-ray diffraction analysis confirmed the formation of the targeted pigment, with major peaks at 25.3 and 32.8° 2θ, demonstrating the potential for an industrially viable, sustainable pigment production process.

利用硫酸盐还原菌假脱硫弧菌可持续合成硫化铈。
本研究研究了一种从采出水中分离出来的新型硫酸盐还原细菌,Pseudodesulfovibrio sp. MCM B-508,因为它具有产生硫化铈(一种工业上有价值的稀土着色剂)的能力。本研究通过克隆和表达该细菌的异同化亚硫酸盐还原酶(dsrAB)基因,在大肠杆菌中证实了色素合成的微生物途径。异化亚硫酸盐还原酶与GenBank数据库中已发表的异化亚硫酸盐还原酶序列同源性最高达94.09%,突出了其新颖性。利用分子工程技术,包括伴侣蛋白与pGro7载体共表达,硫酸盐转化效率显著提高到71.23%,克服了硫酸盐还原细菌过程的局限性。该研究通过重组大肠杆菌菌株成功地将硫酸铈转化为所需的γ -形式硫化铈(Ce2S3),与传统的化学合成方法相比,实现了更高效、更环保的生产方法。x射线衍射分析证实了目标颜料的形成,主要峰位于25.3和32.8°2θ,表明了工业上可行的、可持续的颜料生产工艺的潜力。
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来源期刊
World journal of microbiology & biotechnology
World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.30
自引率
2.40%
发文量
257
审稿时长
2.5 months
期刊介绍: World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.
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