Cadmium removal and indole acetic acid production by ureolytic bacteria isolated from rhizosphere soils.

IF 4.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

World journal of microbiology & biotechnology Pub Date : 2025-08-08 DOI:10.1007/s11274-025-04482-9

Carlos A Adarme-Duran, Elianna Castillo, Pedro F B Brandão

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Abstract

This work reports the isolation of 54 ureolytic bacteria with microbiologically induced carbonate precipitation (MICP) activity from Theobroma cacao L. rhizosphere soils with Cd concentration ranging from 0.49 to 21.29 mg kg^- 1. Analysis of the 16 S rRNA gene showed the isolates belong to the genera Serratia, Pseudomonas, Comamonas, Klebsiella, Bacillus, Citrobacter, Flavobacterium, Delftia, and Stenotrophomonas. They showed ureolytic activity between 0.31 and 1.01 µmol NH₄⁺ mL^- 1 h^- 1 and produced the phytohormone indole acetic acid (IAA). Interestingly, Cd was observed to increase ureolytic activity and did not affect the IAA production of various isolates. Cadmium removal in solution ranged from 4.4 to 87.0% after 48 h. Strains Serratia sp. 89a, Klebsiella sp. 76 h, and Stenotrophomonas sp. 67w exhibited the best performance (> 80%). Cadmium removal through MICP by the genera Delftia, Flavobacterium, and Klebsiella is reported for the first time. Results suggest the isolated bacteria have the potential for Cd bioremediation and could be used to alleviate metal-induced stress through IAA production.

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根际土壤中分离的解脲菌除镉及产吲哚乙酸。

从Cd浓度为0.49 ~ 21.29 mg kg- 1的可可根际土壤中分离到54株具有微生物诱导碳酸盐沉淀（MICP）活性的解尿细菌。16s rRNA基因分析表明，分离株属沙雷氏菌属、假单胞菌属、单胞菌属、克雷伯氏菌属、芽孢杆菌属、柠檬酸杆菌属、黄杆菌属、Delftia属和窄养单胞菌属。它们在0.31 ~ 1.01µmol NH4+ mL- 1 h- 1之间具有溶尿活性，并产生植物激素吲哚乙酸（IAA）。有趣的是，观察到Cd增加了各种分离株的溶尿活性，而不影响IAA的产生。48 h后对镉的去除率为4.4 ~ 87.0%，其中沙雷氏菌89a、克雷伯氏菌76h和窄养单胞菌67w的去除率最高，达到80%。Delftia属、Flavobacterium属和Klebsiella属通过MICP去除镉是首次报道。结果表明，分离的细菌具有Cd生物修复的潜力，可以通过产生IAA来缓解金属诱导的应激。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.30

自引率

2.40%

发文量

257

审稿时长

2.5 months

期刊介绍： World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.