World journal of microbiology & biotechnology最新文献

{"title":"Enhanced L-serine production in Corynebacterium glutamicum via promoter engineering and Bayesian optimization.","authors":"Yujie Gao, Anqian Liu, Xiaomei Zhang, Wei Zhang, Xiaogang Wang, Guoqiang Xu, Xiaojuan Zhang, Hui Li, Jinsong Shi, Zhenghong Xu","doi":"10.1007/s11274-025-04401-y","DOIUrl":"10.1007/s11274-025-04401-y","url":null,"abstract":"<p><p>L-serine is a valuable compound in various industries, including medicine, food and cosmetics. Corynebacterium glutamicum is the preferred choice for the fermentation production of amino acids. In this study, to enhance the L-serine titer, we engineered a C. glutamicum strain capable of high production of L-serine by regulating the transcription level of the L-serine synthesis key enzymes (serA, serC and serB). Firstly, the native promoter strengths of serA, serC and serB in strain C. glutamicum A36 (with 31.1 g/L L-serine titer) were evaluated and compared with four known-strength promoters (Pkan, dap-e, A16-1 and dap-e11). The promoter strength ratios were P-serA: P-serC: P-serB = 101.70: 1: 7.69, ranking as P-serC < Pkan < P-serB < dap-e < P-serA < A16-1 < dap-e11. Then, we regulated and enhanced the L-serine synthesis pathway by promoter engineering through two steps. In the first step, the native promoters of serA, serC and serB were replaced with stronger promoters individually, leading to 9 recombinant strains. The highest L-serine titer of A36-serA^dap-e11 reached 35.83 g/L, showing a 17.21% increase compared to the strain A36. In the second step, the native promoters of serC and serB were replaced with stronger promoters in combination based on the strain A36-serA^dap-e11, resulting in 19 recombinant strains. Among the strains, the recombinant strain A36-serA^dap-e11-serC^dap-e11-serB^dap-e11 (renamed as ACB) generated the highest L-serine titer (40.79 g/L), showed a 33.43% increase compared to the parent strain A36. Subsequently, the L-serine titer of strain ACB reached 46.42 g/L through Bayesian optimization, which was 13.80% higher than before. In the 5 L fermentor, the L-serine titer reached 62.23 g/L. This represents the highest reported L-serine production in C. glutamicum through fermentation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"216"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring pigment-producing Streptomyces as an alternative source to synthetic pigments: diversity, biosynthesis, and biotechnological applications. A review.","authors":"Richard Asah-Asante, Lushi Tang, Xiaowei Gong, Shiqi Fan, Chenjun Yan, Joseph Obiri Asante, Qingwei Zeng","doi":"10.1007/s11274-025-04379-7","DOIUrl":"10.1007/s11274-025-04379-7","url":null,"abstract":"<p><p>The increasing health and environmental concerns associated with synthetic pigments have intensified the global search for natural, eco-friendly alternatives. Among microbial sources, Streptomyces, a genus within the class Actinomycetia, has emerged as a prolific source of bioactive pigments with wide-ranging industrial applications. The review provides a comprehensive synthesis of pigment-producing Streptomyces, focusing on their ecological diversity, biosynthetic pathways, and taxonomic relevance. This review has discussed key pigment classes, including melanin, prodiginine, quinone, and actinorhodin, with their bioactive properties, such as antioxidants, antimicrobials, and anticancer. The review further emphasizes recent advancements in synthetic biology, including clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas)-based gene editing, which has facilitated the activation of silent biosynthetic gene clusters (BGCs) that can enhance pigment yield. Additionally, this review discusses the optimization and fermentation protocols, industrial scalability, and the application of pigments in food, pharmaceutical, cosmetic, textile, and environmental sectors. Despite these advancements, critical research gaps persist, particularly in toxicological evaluation, pigment stability under industrial conditions, high-throughput screening of unexplored Streptomyces strains, and the integration of AI-based predictive tools for pathway optimization. Addressing these gaps is essential to unlocking the full potential of Streptomyces-derived pigments to replace harmful synthetic colorants.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"211"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial metabolism of discotic liquid core component-triphenylene; a future xenobiotic.","authors":"S V Nagarathna, T M Chandramouli Swamy, Pooja V Reddy, Anand S Nayak","doi":"10.1007/s11274-025-04450-3","DOIUrl":"10.1007/s11274-025-04450-3","url":null,"abstract":"<p><p>Triphenylene and its derivatives are gaining significant importance due to their role in the production of purely organic columnar discotic liquid crystals, which are used in organic light-emitting diode (OLED) screens and semiconductors. In the current study, Paenibacillus sp. PRNK-6 was evaluated for its ability to degrade the tetracyclic aromatic hydrocarbon, triphenylene. Paenibacillus sp. PRNK-6 is a promising PAH-degrading bacterium that utilizes triphenylene as its sole source of carbon and energy. Within 120 h of incubation, PRNK-6 degraded 89.75% of 100 mgL⁻¹ triphenylene. Metabolite analysis, enzyme assays, and metabolite feeding experiments indicated that triphenylene was completely metabolized without forming any dead-end products. Several triphenylene metabolites were identified, and a metabolic map of triphenylene was constructed based on the characterization of metabolites, and the specific activities of enzymes involved in its degradation.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"203"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring coffee processing stages: Wet fermentation with and without Saccharomyces cerevisiae vs. Conventional process.","authors":"Ana Paula Pereira Bressani, Nádia Nara Batista, Débora Mara de Jesus Cassimiro, Simone da Fonseca Pires, Hélida Monteiro de Andrade, Disney Ribeiro Dias, Rosane Freitas Schwan","doi":"10.1007/s11274-025-04452-1","DOIUrl":"10.1007/s11274-025-04452-1","url":null,"abstract":"<p><p>The present study aimed to perform a wet coffee fermentation process using self-induced anaerobic fermentation (SIAF), with or without a starter culture, and a subsequent pulping stage to verify the influence on the beverage's quality. The ripe fruits were fermented in water using the SIAF method, with and without inoculating S. cerevisiae CCMA 0543, and were compared to a Conventional process (direct drying on suspended terraces). Samples were collected at different times of fermentation and drying for analysis of yeast population (qPCR), carbohydrates and organic acids (HPLC), protein profile (2-D SDS-PAGE), and volatile compounds (GC-MS). Sensory analysis was performed by certified tasters (Q-Graders). The treatment with S. cerevisiae (SC) demonstrated greater efficiency in consuming glucose and fructose, resulting in the lowest concentrations of these sugars at the end of fermentation (1.30 and 2.64 g/Kg, respectively). This treatment also showed increased lactic acid (8.76 g/Kg) and reduced acetic acid (6.40 g/Kg). The Conventional process presented a higher acetic acid content (10.12 g/Kg). Proteomic analysis revealed a unique protein expression profile in the SC treatment. After fermentation, a higher abundance of esters, aldehydes, alcohols, and acids was identified in the inoculated treatment. After roasting, furans, pyrazines, and other volatile compounds were predominant in this coffee. This treatment was the only one classified as specialty coffee (score 85.00). The inoculation of S. cerevisiae using the SIAF method proved effective in enhancing the quality of wet coffee, producing consistent and high-quality beverages that meet specialty coffee standards.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"212"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From cultivation challenges of Acidobacteriota to biotechnological promises - unveiling what is needed to fully harness their potential.","authors":"Osiel Silva Gonçalves, Alexia Suellen Fernandes, Thais Gonzaga Gontijo de Sousa, Mateus Ferreira Santana, Cristine Chaves Barreto","doi":"10.1007/s11274-025-04433-4","DOIUrl":"10.1007/s11274-025-04433-4","url":null,"abstract":"<p><p>Acidobacteriota are currently well-known for their ubiquity and metabolic versatility. Their environmental importance is the subject of many studies, but their biotechnological applications still need to be explored. Although they are considered hard-to-culture bacteria, many genomes are available, which reveals promising biotechnological potential. Despite these promising features, the application of Acidobacteriota in biotechnology remains underexplored. In this context, we review key findings regarding the potential applications of Acidobacteriota, emphasizing advancements derived from genomic, metagenomic, and culture-based studies. We specifically focus on their ability to produce enzymes and other bioactive molecules, their antimicrobial properties, and their potential applications in agriculture and bioremediation. Furthermore, we discuss strategies to overcome the challenges associated with culturing and manipulating these bacteria, such as heterologous expression and other emerging techniques related to -omics and computational approaches. These strategies could provide deeper insights into Acidobacteriota's metabolism, including their biosynthetic pathways and interactions within microbial consortia. Ultimately, this understanding could lead to broader applications of Acidobacteriota in environmental and industrial biotechnology.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"208"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating antimicrobial resistance: a closer look at disrupting Escherichia coli metabolism and motility with biogenic silver nanoparticles.","authors":"Moumita Sil, Shauryabrota Dalui, Abhishek Choudhury, Arunava Goswami, Arindam Bhattacharyya","doi":"10.1007/s11274-025-04428-1","DOIUrl":"10.1007/s11274-025-04428-1","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) poses a dire global threat, with bacterial resistance predicted to cause 10 million deaths annually by 2050 if left unaddressed. Among the leading contributors, Escherichia coli ranks as a critical priority pathogen, driving the need for innovative strategies to combat resistance. This study addresses the urgent demand by exploring the antibacterial efficacy and mode of action of biogenic silver nanoparticles (AgNPs) synthesized using Emblica officinalis. AgNPs, characterized as spherical (average size: 46.5 nm) with high stability (zeta potential: -39.02 mV) and photoluminescent properties, exhibited potent antimicrobial activity, with minimum inhibitory concentrations ranging from 9.76 to 19.53 ppm. RNA-seq analysis revealed substantial metabolic and regulatory shifts in E. coli upon AgNP exposure, including upregulation of arginine biosynthesis, metabolism, and transport genes (e.g., argC, argB, argA, artJ), which supported ATP production via the Arginine Deiminase (ADI) pathway and enhanced stress resilience through polyamine synthesis. Upregulation of carA showed the interconnectedness of arginine and pyrimidine biosynthesis under energy-depleted conditions. Simultaneously, downregulation of motility-related genes (flhDC, fliC) disrupted flagellar biosynthesis, rendering E. coli non-motile and more vulnerable to oxidative stress. This was linked to reduced cAMP-CRP complex activity and arginine-sodium competition at flagellar motors. Structural analyses confirmed a face-centered cubic crystalline structure and functionalization with biomolecules, enhancing biocompatibility. Cytotoxicity assays on MDA-MB-231 cells demonstrated dose-dependent reductions in cell viability, with manageable safety profiles. These findings highlight biogenic AgNPs as eco-friendly, effective antimicrobial agents exploiting bacterial metabolic disruption and impaired motility to counter resistance, offering promising solutions to mitigate the AMR crisis.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"222"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-mediated genome editing in Ganoderma lucidum: recent advances and biotechnological opportunities.","authors":"Dongmei Lv, Yan Xu, Zi-Xu Wang, Qi-Lin Zhang, Jin-Ping Yan, Jun-Wei Xu","doi":"10.1007/s11274-025-04458-9","DOIUrl":"10.1007/s11274-025-04458-9","url":null,"abstract":"<p><p>Ganoderma lucidum is a well-known traditional medicinal mushroom that has attracted considerable attention due to its potential as a promising cell factory for producing high-value bioactive compounds. However, conventional methods for the genetic manipulation of G. lucidum are often time-consuming and labor-intensive, hindering research into the biosynthesis and regulatory mechanisms of its valuable natural products. In recent years, the clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9) system has emerged as a powerful genome editing tool, extensively utilized in life sciences research due to its high efficiency and user-friendliness. This review provides a structured overview of advancements in CRISPR/Cas9-mediated genome editing technology in G. lucidum. We first discuss the development and optimization of the CRISPR system, focusing on the various expression strategies for Cas9 and guide RNA established in G. lucidum. Furthermore, we highlight the application of this system for targeted gene deletion, insertion, and replacement in genome editing, as well as its use in the functional analysis of genes in G. lucidum. In addition, we discuss the limitations and challenges associated with employing CRISPR/Cas9 tools in G. lucidum and provide an outlook on the future development of the CRISPR/Cas9 system and its applications in this organism.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"223"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial populations under fluoride stress: a metagenomic exploration from Indian soil.","authors":"Krishnendu Pramanik, Arup Sen, Subrata Dutta, Gouranga Sundar Mandal, Bappa Paramanik, Arpita Das, Nitin Chatterjee, Ankit Kumar Ghorai, Md Nasim Ali","doi":"10.1007/s11274-025-04408-5","DOIUrl":"10.1007/s11274-025-04408-5","url":null,"abstract":"<p><p>Fluoride exposure, even at a low concentration, significantly impairs crop growth and productivity by inhibiting metabolic enzymes and disrupting photosynthesis. Addressing this challenge, microbial de-fluoridation emerges as a vital strategy to improve soil health, enhance crop growth, and ensure agricultural sustainability. This study analyzed topsoil samples (0-0.2 m depth) from rice fields in three blocks of Purulia district, West Bengal-Arsha, Jhalda-I, and Joypur. Fluoride content in the samples ranged from 58.76 ± 0.76 mg/kg to 282.9 ± 4.9 mg/kg (total) and 1.57 ± 0.02 mg/kg to 2.97 ± 0.03 mg/kg (available). The metagenomic analysis of the collected soil samples revealed diverse microbial communities comprising archaea, bacteria, fungi, and viruses, with Actinobacteria (phylum), Hyphomicrobiales (order), and Nocardioidaceae (family) being the dominant prokaryotes. Arsha soil with comparatively low fluoride contamination exhibited the highest microbial diversity (11,891 taxa), followed by Joypur (11,528 taxa) and Jhalda-I (11,358 taxa), with Arsha showing nearly double the unique microbial taxa compared to the other locations. Clusters of orthologous groups of proteins functional analysis identified 60,898 genes in Arsha, 63,403 genes in Jhalda-I, and 73,334 genes in Joypur, while Kyoto encyclopedia of genes and genomes analysis revealed 9,385, 9,104, and 10,633 genes, respectively. Key genes associated with fluoride metabolism-inorganic pyrophosphatase, divalent metal cation transporter mntH, and putative fluoride ion transporter crcB-were abundant across all sites, highlighting the influence of fluoride on microbial community structure. This study provides the first comprehensive report on soil microbial communities in fluoride-rich areas, highlighting the potential of native fluoride-tolerant microbes to mitigate fluoride toxicity in agricultural soils and offer sustainable, microbe-based solutions to fluoride contamination.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"221"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carvacrol induces apoptosis in Aspergillus niger through ROS burst.","authors":"Qingchao Gao, Zhiruo Feng, Zuli Wang, Fangyuan Zhao, Jian Ju","doi":"10.1007/s11274-025-04437-0","DOIUrl":"10.1007/s11274-025-04437-0","url":null,"abstract":"<p><p>Carvacrol, a phenolic monoterpenoid and major active constituent of plant essential oils, exhibits broad-spectrum antimicrobial activity with potential applications in food preservation and agricultural biocontrol. Previous studies have shown that one of the antifungal mechanisms of carvacrol may be the induction of reactive oxygen species (ROS) burst, but its specific action mechanism is still unclear. This study investigated the apoptosis mechanism of Aspergillus niger caused by ROS burst induced by carvacrol in the molecular level. Carvacrol induced ROS burst, leading to the accumulation of intracellular hydrogen peroxide (H₂O₂) concentration in A. niger which caused the cellular oxidative stress and lipid peroxidation, leading to an increase in the content of unsaturated fatty acids as well as a decrease in the content of glycerophospholipids in the cell membrane, and then disrupted the integrity of cell membranes. RT-qPCR was used to analyze the expression levels of different subunit genes of NADPH oxidase, and the result showed that NoxA, Cdc24, NoxD and NoxR were all upregulated to varying degrees. And the inhibitory effect of carvacrol on A. niger can be alleviated by adding an additional ROS scavenger. Meanwhile, NADPH oxidase inhibitors eliminated the ROS production induced by carvacrol. In summary, carvacrol can induce ROS burst in A. niger by regulating the gene expression levels of different subunits of NADPH oxidase, leading to membrane lipid peroxidation, irreversible damage to the cell membrane, the disruption of redox dynamic balance and finally cell death.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"225"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gut microbiota-bile acid axis: a crucial regulator of immune function and metabolic health.","authors":"Anuradha Tyagi, Vinay Kumar","doi":"10.1007/s11274-025-04395-7","DOIUrl":"10.1007/s11274-025-04395-7","url":null,"abstract":"<p><p>The gut microbiota and bile acid metabolism are intricately linked, playing a crucial role in immune regulation, metabolic processes, and overall health. The gut microbiome, consisting of diverse bacterial genera such as Bacteroides, Clostridium, Lactobacillus, Bifidobacterium, and Eubacterium, facilitates the conversion of primary bile acids into secondary bile acids through enzymatic modifications. Bile acids, synthesized in the liver and modified by gut microbiota, act as signaling molecules that regulate immune responses via bile acid receptors, including the farnesoid X receptor (FXR), G protein-coupled bile acid receptor 1 (GPBAR1), pregnane X receptor (PXR), vitamin D receptor (VDR), and sphingosine-1-phosphate receptor 2 (S1PR2). Dysbiosis-an imbalance in gut microbial composition-disrupts bile acid metabolism, leading to impaired activation of bile acid receptors and contributing to various diseases. These include inflammatory bowel disease, metabolic disorders such as obesity and type 2 diabetes, autoimmune diseases like multiple sclerosis, and liver conditions such as cholestasis and non-alcoholic fatty liver disease. Dysfunctional bile acid receptor signaling further promotes chronic inflammation, metabolic dysregulation, and disturbances in gut-liver-immune homeostasis. Emerging therapeutic strategies targeting bile acid receptors, restoring microbiota balance, and implementing dietary interventions offer promising avenues for disease prevention and management. This review explores the pivotal role of gut microbiota in modulating immune responses through bile acid receptors and highlights their therapeutic potential in improving treatment outcomes.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 7","pages":"215"},"PeriodicalIF":4.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}