World journal of microbiology & biotechnology最新文献

{"title":"HOM2 and TPK1 are associated with Kluyveromyces marxianus growth under high-temperature and reduced-aeration conditions.","authors":"Huan Wang, Zhongke Sun, Zifu Ni, Yanli Qi, Xianyang Feng, Yini Shi, Hongkun Xiao, Chengwei Li","doi":"10.1007/s11274-026-05267-4","DOIUrl":"10.1007/s11274-026-05267-4","url":null,"abstract":"<p><p>Kluyveromyces marxianus (K. marxianus) holds significant application potential in biomanufacturing. However, in industrial processes, high temperature and reduced aeration often occur and severely constrain its fermentation performance. The molecular mechanisms underlying yeast responses to these stresses remain to be fully elucidated. In this study, transcriptomic analysis combined with Weighted Gene Co-expression Network Analysis (WGCNA) was performed to characterize the global gene expression profiles of K. marxianus under high-temperature or reduced-aeration conditions. Co-expression network and qPCR expression analysis identified two core hub genes, TPK1 encoding the catalytic subunit of cAMP-dependent protein kinase, and HOM2 encoding aspartate-semialdehyde dehydrogenase. The gene knockout experiments revealed that deletion of TPK1 or HOM2 lead to reduced maximum specific growth rate (µ_max), decreased cell wall integrity and exacerbated membrane damage. Conversely, overexpression of these genes significantly increased µ_max and enhanced stress tolerance. By genetic and chemical comparisons, this study identified TPK1 and HOM2 as candidate hub genes involved in yeast adaptation to high temperature and reduced aeration conditions, providing new insights into yeast's stress-responsive regulatory mechanisms and offering valuable key genetic targets for the engineering of robust industrial yeast chassis.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifaceted biological activities of linalool: antimicrobial, antibiofilm, cytotoxic, antiproliferative and caspase-3/7 activity.","authors":"Esra Bulut Atalay, Rukiye Aslan","doi":"10.1007/s11274-026-05286-1","DOIUrl":"10.1007/s11274-026-05286-1","url":null,"abstract":"<p><p>Linalool is a natural bioactive monoterpene with antimicrobial, antibiofilm, and anticancer potential. Its multifaceted biological activity was investigated using microbial and mammalian cell-based models, addressing the limited integrated characterization of these effects. In this study, the antimicrobial and antibiofilm efficacy of linalool was evaluated against selected pathogens, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, methicillin-resistant S.aureus (MRSA), Klebsiella pneumoniae, and Candida albicans. Its cytotoxic, antiproliferative, and morphological effects were evaluated in the C6 glioma cell line at concentrations of 25, 50, 100, 200, 400, and 800µM, together with assessment of caspase-3/7 activity as an apoptosis-related marker. Linalool exhibited antimicrobial activity against all tested microorganisms and significantly suppressed biofilm formation. Among the tested strains, S. aureus was the most susceptible pathogen, showing the lowest minimum inhibitory concentration (MIC) (414.8µM) and the highest biofilm inhibition (64.77%). MTT analysis showed that linalool exerted dose-dependent cytotoxicity in C6-glioma cells after 24 h, with an IC₅₀ of 563.3 µM (95%CI:370.3-871.7µM). Longitudinal trypan blue exclusion counting revealed that 400 µM linalool produced the strongest antiproliferative effect over 4 days, resulting in a 1.6-fold reduction in viable C6 cell numbers compared with the untreated control, accompanied by distinct morphological alterations. In addition, treatment with 400µM linalool increased executioner caspase-3/7 activity by up to 2.07-fold, which may be consistent with the involvement of apoptosis-related processes in linalool-induced cytotoxicity. These findings demonstrate the multifaceted biological activity of linalool, encompassing antimicrobial, antibiofilm, cytotoxic, and antiproliferative effects, with increased caspase-3/7 activity observed after linalool treatment, warranting further investigation of the underlying mechanisms.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkali-treated Phragmites karka as a sustainable support for immobilized Anoxybacteroides rupiense UE27 in laccase production and azo dye detoxification.","authors":"Muhammad Faisal, Uroosa Ejaz, Tahira Mohsin Ali, Mohammed Alorabi, Amal S Alswat, Muhammad Sohail","doi":"10.1007/s11274-026-05270-9","DOIUrl":"10.1007/s11274-026-05270-9","url":null,"abstract":"<p><p>Laccases are multi-copper oxidoreductases with broad industrial utility, however, their practical application is often limited by high production costs, low yields, and poor operational stability. Immobilizing laccase-producing bacterial cells on sustainable, cost-effective matrices, such as halophyte Phragmites karka offers a robust strategy to overcome these challenges by improving cellular stability and ensuring cell reusability for enzyme production. This study optimizes a novel immobilization system for Anoxybacteroides rupiense UE27 to achieve enhanced laccase titers and high-efficiency detoxification of carcinogenic synthetic dyes. Analysis of six factors for immobilization and laccase production through Plackett-Burman design (PBD) showed 16.45% (w/w) immobilization efficiency and 23 IU L^-1 laccase. Agitation, number of matrix pieces, incubation, inoculum, and temperature were found to be significant and further optimized using a Central Composite design (CCD). Under optimized conditions, although the immobilization was 13.2%, the laccase titers increased to 37 IU L^-1 in the third repeated cycle. The optimized immobilization system removed 100% and 99% of 100 ppm malachite green and congo red within 12 h of incubation, respectively. The experiment on the growth of Saccharomyces cerevisiae in presence of treated malachite green and congo red demonstrated the successful detoxification of dye solution. The mechanical analysis showed that alkaline treatment significantly increased the compression force from 46.35 N to 54.26 N and fracture strength from 983.48 kPa to 1148.29 kPa in comparison to the untreated matrix. Scanning electron microscopy further confirmed bacterial immobilization on the matrix surface. These results demonstrate the potential of Phragmites karka stem matrices as a cost-effective support matrix for bacterial laccase production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of free and immobilized Scenedesmus obliquus cells in desalination of brackish water.","authors":"Rania Omar, Mahmoud S M Abdel Wahed, Sherif H M Hassan, Ibraheem B M Ibraheem, Khaled N M Elsayed","doi":"10.1007/s11274-026-05234-z","DOIUrl":"10.1007/s11274-026-05234-z","url":null,"abstract":"<p><p>Sustainable desalination has increased interest in microalgal biotreatment, yet immobilized microalgae performance under high salinity remains poorly characterized. This study compared free-cell suspensions and agar-immobilized Scenedesmus obliquus for NaCl removal from synthetic brackish water (10-30 g/L NaCl) over 12 days. Free-cell treatments achieved 0.66-2.92 g/L removal (3.61-11.72%). In contrast, the algae-agar bead system showed superior performance and temporal consistency, at 30 g/L NaCl, the 1:5 bead-to-solution ratio achieved the highest TDS-based removal of 4.51 g/L (18.36%), 1.54-fold higher than the 15-mL free-cell treatment (2.92 g/L). Blank agar beads showed no removal, confirming agar as an inert scaffold. While TDS trends were consistent, the 15-mL free-cell treatment achieved a slightly higher EC reduction (11.79%) than the 1:5 beads (11.00%) at 30 g/L. FTIR and SEM/EDX analyses revealed marked surface damage and salt encrustation in free cells, with Na signals increasing by 5.30% points and C signals decreasing by 6.25% points. These findings support biosorption and surface-associated salt deposition rather than intracellular sequestration. The apparent adsorption isotherms models indicated heterogeneous uptake in free cells (Freundlich, R² ≥ 0.999), while immobilized cells (1:5) showed moderate agreement with the Langmuir model (R² = 0.853), tentatively suggesting monolayer adsorption. Overall, immobilization improved TDS removal and consistency, though free cells remained competitive in EC reduction at high salinities. This study highlights the potential of immobilized systems for robust biodesalination in challenging saline environments.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZymoR: Bridging plant pathology and automated bioinformatics for fungicide resistance profiling in Zymoseptoria tritici.","authors":"Mateusz Maździarz, Katarzyna Bilska, Katarzyna Krawczyk, Patryk Wiśniewski, Paulina Staniszewska, Tomasz Kulik","doi":"10.1007/s11274-026-05287-0","DOIUrl":"10.1007/s11274-026-05287-0","url":null,"abstract":"<p><p>Zymoseptoria tritici, the causal agent of Septoria tritici blotch (STB), is one of the most destructive fungal pathogens of wheat worldwide. The extensive use of fungicides has driven the emergence of diverse resistance mechanisms involving multiple target-site genes, as well as non-target-site resistance. High-throughput sequencing (HTS) technologies now enable large-scale detection of resistance-associated genetic variation, yet routine implementation of HTS-based monitoring remains constrained by the lack of standardized analytical workflows and tools that translate sequence data into biologically meaningful resistance information. Here, we present ZymoR, a dedicated bioinformatics platform for automated molecular surveillance of fungicide resistance in Z. tritici. ZymoR integrates sequence quality assessment, mutation detection and CYP51 haplotype classification within a single user-friendly workflow. The platform incorporates a curated and expandable database of resistance-associated variants across the principal fungicide target genes, enabling standardized annotation of known resistance markers while facilitating the identification of previously undescribed variants. Unlike conventional variant-calling pipelines, ZymoR links detected genetic variation to standardized nomenclature and resistance-associated metadata, substantially reducing the bioinformatic expertise required for data interpretation. The ZymoR package was made available as an open-source tool via GitHub at https://github.com/Mordziarz/ZymoR .</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13593640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149017441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosa damascena-derived exosome-like nanovesicles inhibit Salmonella enterica via membrane disruption, oxidative stress, and virulence suppression.","authors":"Subhashini Brahadeeswaran, Ramasamy Tamizhselvi","doi":"10.1007/s11274-026-05274-5","DOIUrl":"10.1007/s11274-026-05274-5","url":null,"abstract":"<p><p>Plant-derived exosome-like nanoparticles (P-DENs) have emerged as promising bio-nanocarriers with antimicrobial potential. In this study, we isolated and characterized exosome-like nanovesicles from Rosa damascena and evaluated their antibacterial efficacy against Salmonella enterica, a significant foodborne pathogen. Rosa damascena-derived exosomes-like nanovesicles (R-DENs) exhibited 8.9 × 10⁹ particles/mL in nanoparticle tracking analysis. Growth assays revealed significant inhibition of bacterial proliferation (63.52%). Propidium iodide (PI) staining in flow cytometry demonstrated concentration-dependent bacterial cell death (65.78%), while scanning electron microscopy (SEM) visualized distinct ultrastructural damage to the bacterial envelope, confirming membrane disruption as a primary antibacterial mechanism. Consistent with these effects, R-DENs markedly reduced biofilm formation (69.88%) and suppressed exopolysaccharide (EPS) production (69.10%). Elevated intracellular reactive oxygen species (ROS) levels (up to 65.37%) further indicated oxidative stress as a major antibacterial contributor. Moreover, R-DENs treatment impaired bacterial adhesion (77.88%) and invasion (67.37%) in L929 fibroblasts, correlating with attenuation of pathogenicity. Gene expression analysis confirmed significant downregulation of biofilm- and virulence-associated genes (fimA, csgD, bcsA, rsmA, and rpoS), linking the phenotypic changes to molecular interference with pathogenic pathways. Collectively, these findings demonstrate that R-DENs exert multifaceted antibacterial activity through membrane disruption, biofilm and EPS inhibition, ROS generation, and suppression of virulence traits, underscoring their potential as natural, biocompatible nanotherapeutics against drug-resistant bacterial infections.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a lactose-complemented antibiotic-free host-vector platform for stable enzyme expression in Limosilactobacillus fermentum 217 - 82.","authors":"Ruize Xu, Junlin Li, Fangyu Guo, Weiyan Wang, Chen Yang, Zhenshang Xu, Ting Wang","doi":"10.1007/s11274-026-05266-5","DOIUrl":"10.1007/s11274-026-05266-5","url":null,"abstract":"<p><p>Food-grade microbial bioprocessing requires genetically stable and antibiotic-free host-vector systems that are compatible with food-related applications. However, the use of Limosilactobacillus fermentum as a biomanufacturing chassis is limited by inefficient DNA delivery and the lack of strain-adapted food-grade selection strategies. In this study, a proof-of-concept, lactose-complemented antibiotic-free host-vector framework was established in L. fermentum 217 - 82. An efficient electrotransformation protocol was first established as an enabling step for host engineering, yielding a transformation efficiency of 7.50 × 10⁵ CFU/µg DNA. The genes lacM and lacLM were individually targeted to validate their involvement in lactose utilization, and subsequent gene inactivation experiments confirmed their utility as effective markers for metabolic complementation. Antibiotic-free shuttle vectors were constructed by combining lacZα-assisted plasmid screening in E. coli with host-specific lacM/lacLM-mediated lactose complementation in L. fermentum. The resulting recombinant strains maintained plasmid stability during serial passage and supported functional heterologous expression of cis-proline 4-hydroxylase. Functional validation using cis-proline 4-hydroxylase confirmed that the platform supported whole-cell L-proline-to-hydroxyproline bioconversion, with hydroxyproline production reaching 147.62 µg/mL. These findings demonstrate the feasibility of integrating lactose-dependent selection, stable antibiotic-free plasmid maintenance, functional heterologous enzyme expression, and whole-cell bioconversion within a strain-adapted host-vector framework in L. fermentum 217 - 82. Although broader applicability remains to be validated using additional heterologous proteins and process conditions, the established framework provides a methodological basis for the future development of more generally applicable antibiotic-free expression platforms in L. fermentum.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond monoculture limits: a critical review of yeast co-cultures for xylitol production from lignocellulosic biomass : Yeast co-cultures for xylitol production.","authors":"W Monroy-Martínez, J C González-Hernández, M C Chávez-Parga","doi":"10.1007/s11274-026-05268-3","DOIUrl":"10.1007/s11274-026-05268-3","url":null,"abstract":"<p><p>The biotechnological production of xylitol from lignocellulosic biomass residues offers a sustainable alternative to traditional chemical synthesis; however, its industrial implementation is limited by the metabolic constraints of conventional microbial monocultures. This review examines the limitations of monocultures, highlighting how redox cofactor imbalances, glucose-induced catabolic repression, and the toxicity of furanic inhibitors and organic acids in crude hydrolysates create energetic and metabolic challenges that reduce yields and volumetric productivity. To overcome these biological limitations, it is necessary to shift toward co-culture engineering based on the principle of division of labor (DoL). Key synergistic mechanisms, including in situ detoxification, parallel utilization of pentose and hexose mixtures, and metabolite exchange (cross-feeding), which distribute metabolic functions among specialized strains, expanding metabolic diversity and optimizing carbon valorization, are analyzed. Implementing control strategies that maintain coordinated fluxes is critical to positioning consortia as a viable and profitable platform for xylitol production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation-dependent sugar regulation enhances biofilm-mediated stability and bioactivity of Bacillus thuringiensis formulations.","authors":"Lan Chen, Wang Ke, Hongxin Su, Zixuan Wang, Yufei Mao, Zhongzeng Su, Linke Zhang, Yan Guo, Xiaohui Hong, Hui Ye, Ruizhe Feng, Lingyi Luo, Fuyong Lin, Tianpei Huang","doi":"10.1007/s11274-026-05280-7","DOIUrl":"10.1007/s11274-026-05280-7","url":null,"abstract":"<p><p>Sugars are important carbon sources involved in microbial growth and functional regulation. However, how sugars with different structural characteristics regulate microbial pesticide performance in complex formulation systems remains unclear. In this study, Bacillus thuringiensis (Bt) formulations were used to systematically evaluate the effects of different sugars on functional traits. The results showed pronounced type-dependent regulatory effects. Sugars with distinct structural features modulated biofilm formation by affecting cell adhesion and extracellular polysaccharide production. An optimized sugar combination increased biofilm yield by approximately tenfold. Certain sugars further enhanced UV tolerance and insecticidal activity. For example, pectin increased the survival rate of suspension concentrates by 5.3-fold after 4 h of UV exposure, while glucose, glycerol, and maltose improved insecticidal activity by 2.72-3.91-fold. Further analysis indicated that sugar structural characteristics are critical determinants of their regulatory effects, influencing bacterial surface interactions and the microenvironment. This biofilm-centered mechanism showed consistency across different formulation types. Overall, this study provides new insights into the development of stable and efficient Bt-based biopesticides.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asynchronous recovery of canonical ammonia oxidizers and comammox drives post-drought N₂O emissions in contrasting soils.","authors":"Yuehang Zhu, Shuoshuo Wu, Jun Li, Baowei Hu, Guixin Chu, Rui Tao","doi":"10.1007/s11274-026-05260-x","DOIUrl":"10.1007/s11274-026-05260-x","url":null,"abstract":"<p><p>Intensifying drought regimes driven by global climate change pose a severe threat to soil nitrogen cycling stability. While the metabolic responses of microbes to stress are documented, the mechanisms linking the differential resilience of nitrifying guilds to post-drought nitrous oxide (N₂O) pulses remain elusive. Here, we investigated the recovery dynamics of ammonia-oxidizing archaea (AOA), bacteria (AOB), and comammox Nitrospira alongside temporal N₂O emission pulses in contrasting acidic and alkaline soils following common drought and extreme drought. Treatment effects and community structural divergence were evaluated using permutational multivariate analysis of variance (PERMANOVA). Post-drought response patterns differed among guilds: AOA and AOB exhibited marked population fluctuations, signaling a resilience-based recovery, whereas comammox Nitrospira demonstrated greater abundance stability, maintaining stable abundance in both soils throughout the 60-day incubation. Community reassembly was primarily governed by soil pH, which acted as a divergent environmental filter; specifically, acidic conditions favored the dominance of AOA and comammox, whereas alkaline conditions selectively promoted AOB proliferation. Critically, N₂O emissions correlated with recovery patterns among the specific nitrifier guilds. Random forest modeling demonstrated that comammox was the primary driver of emissions during the rapid \"early recovery\" phase of rewetting, while the delayed resurgence of AOA and AOB sustained N₂O production in the later stages, revealing the asynchronous recovery of these guilds modulated the temporal duration of nitrification-derived N₂O fluxes. This study highlights how the temporal niche differentiation of nitrifiers regulates the magnitude and duration of nitrogen losses following extreme drought events.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"42 10","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}