World journal of microbiology & biotechnology最新文献

{"title":"Unveiling the potential of Loigolactobacillus coryniformis BCH-4 to ameliorate drought stress in Zea mays L.","authors":"Anam Tariq, Mahwish Salman, Muhammad Arslan Ashraf, Muhammad Rizwan Javed, Abdul Tawab","doi":"10.1007/s11274-025-04267-0","DOIUrl":"10.1007/s11274-025-04267-0","url":null,"abstract":"<p><p>The lactic acid bacterial (LAB) species have proven multifaceted roles in sustainable agriculture due to their biologically safe nature, making them eco-friendly. However, their plant growth-improving mechanisms in stressed and non-stressed conditions are still under consideration. Thus, the current work has been planned to evaluate the drought tolerance potential and plant growth-promoting (PGP) traits of Loigolactobacillus coryniformis BCH-4 in Zea mays L. This bacterium was tolerant in the presence of 30% PEG. It also exhibited auxin synthesis, ammonia production, and phosphate solubilization potential. Based on these findings, a pot experiment was conducted with selected drought-tolerant maize seeds (Sawari Kashmir) and drought-sensitive seeds (Malka-2016), bioprimed with L. coryniformis under 2 water regimes; 100% field capacity (optimal watering condition) and 50% field capacity (a drought stress condition). Our results manifested an upsurge in maize growth, photosynthetic pigments, and nutrient uptake in bioprimed seeds under stressed conditions. Besides, biopriming considerably improved nitrate reductase levels alongside higher nitric oxide and hydrogen sulphide activity. This treatment also improved drought-induced oxidative stress tolerance in maize plants by promoting osmolytes and antioxidant activities. Further, the GC-MS analysis revealed various drought-tolerant metabolites, known to have plant growth-promoting potential i.e., 1-hexadecene, tetradecane, hexadecanoic acid, 15-methyl-, methyl ester, and hexadecane, produced by L. coryniformis. Remarkably, these metabolites synergistically improve maize growth and tolerate the drought. This study publicized an extensive variety of metabolites, produced by L. coryniformis liable for plant growth promotion and drought tolerance.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"49"},"PeriodicalIF":4.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative microbial activators for enhanced bioremediation of oil-contaminated soils: mechanistic insights.","authors":"Yating Deng, Wujuan Sun, Yongbin Li, Jun Wei, Ruirui Rao, Qiongyu Cao, Sichang Wang, Qunzheng Zhang, Congyu Ke","doi":"10.1007/s11274-025-04258-1","DOIUrl":"10.1007/s11274-025-04258-1","url":null,"abstract":"<p><p>This paper developed an efficient microbial activator formula and conducted an in-depth study on its efficacy and mechanism in promoting the degradation of petroleum hydrocarbons in oil-contaminated soil. A 60-day microbial remediation experiment conducted on oily soil revealed that the microbial activators significantly boosted the activities of dehydrogenase and catalase, subsequently speeding up the degradation of petroleum hydrocarbons in the soil. The overall degradation rate reached as high as 71.23%, with the most significant degradation effect observed in asphaltenes, achieving a degradation rate of 93.98%. This was followed by aromatic hydrocarbons (90.45%), saturated hydrocarbons (84.39%), and asphaltenes (65%). Compared to traditional microbial stimulation methods, this activator demonstrated significant superiority. Microbial diversity analysis reveals that microbial activators can effectively activate microbial activity in soil targeting refractory petroleum hydrocarbon components. By comparing the changes in microbial community structure before and after the addition of microbial activators, we found that the activators promoted an increase in the abundance of microorganisms belonging to the Bacillota, Pseudomonadota, and Bacteroidetes, which have petroleum hydrocarbon degradation functions, and facilitated the evolution of microbial community structure towards a direction more conducive to petroleum hydrocarbon degradation. KEGG metabolic pathway analysis revealed that the degradation pathways for alkanes, aromatic hydrocarbons, and PAHs are primarily present in these bacterial phylum. This research not only clarifies the degradation mechanism but also supports future bioremediation efforts.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"47"},"PeriodicalIF":4.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in engineering non-native microorganisms for poly(3-hydroxybutyrate) production.","authors":"Said Nawab, Muhammad Wajid Ullah, Syed Bilal Shah, Ya-Fei Zhang, Hareef Ahmed Keerio, Yang-Chun Yong","doi":"10.1007/s11274-025-04261-6","DOIUrl":"10.1007/s11274-025-04261-6","url":null,"abstract":"<p><p>Poly(3-hydroxybutyrate) (PHB) is a biodegradable polymer that belongs to a group of polymers called polyhydroxyalkanoates (PHAs). PHB can be synthesized from renewable resources, making it a promising alternative to petroleum-derived plastics. It is also considered non-toxic, biodegradable, and biocompatible, which makes it suitable for various applications in the medicine and biomedicine. Many microorganisms biosynthesize and accumulate PHB naturally. However, recent advancements in metabolic engineering and synthetic biology have allowed scientists to engineer non-native microorganisms to produce PHB. This review comprehensively summarizes all non-native microbial hosts used for PHB biosynthesis and discusses different metabolic engineering approaches used to enhance PHB production. These strategies include optimizing the biosynthesis pathway through cofactor engineering, metabolic pathway reconstruction, and cell morphology engineering. Moreover, the CRISPR/Cas9 approach is also used for manipulating the genome of non-host microorganisms to enable them produce PHB. Among non-native microbial hosts, Escherichia coli has been successfully used for industrial-scale PHB production. However, further genetic engineering approaches are needed to make non-native microbial hosts more suitable for large-scale PHB production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"48"},"PeriodicalIF":4.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global landscape of Vibrio parahaemolyticus research: a bibliometric analysis.","authors":"Nor Hafizah Zakaria, Noorul Darlina Edlin Abd Rahim, Nur Fathiah Rosilan, Yeong Yik Sung, Khor Waiho, Sarahani Harun, Rabiatul Adawiah Zainal Abidin, Nor Afiqah-Aleng","doi":"10.1007/s11274-025-04262-5","DOIUrl":"10.1007/s11274-025-04262-5","url":null,"abstract":"<p><p>Vibrio parahaemolyticus poses a notable threat to marine ecosystems and can cause infections and disease outbreaks in seafood species, which can affect humans upon consumption. The global impacts of such infections and outbreaks on human and animal health led to a growing number of studies from various countries discussing the prevention, control, treatment, and overall implications of V. parahaemolyticus. Hence, this study aims to retrieve relevant studies on V. parahaemolyticus using a bibliometric analysis to understand current research status, trends, and hotspots regarding this bacteria. Relevant literature was searched across the Scopus database, and the data were subsequently analyzed using Biblioshiny software. In addition, a manual examination was conducted to identify the hosts of V. parahaemolyticus and diseases caused by the bacteria. Overall, 7,096 records were obtained from Scopus from 1963 to 2023. A bibliometric analysis identified 17,220 authors, with China emerging as the global leader. The analysis also highlighted significant keywords such as \"Vibrio parahaemolyticus,\" \"Litopenaeus vannamei,\" and \"innate immunity,\" suggesting a focus on the impact of V. parahaemolyticus on L. vannamei, specifically emphasizing the shrimp's innate immune responses. Host-disease interaction network also uncovered 53 interactions between hosts and diseases involving L. vannamei or Penaeus vannamei as the primary host, with acute hepatopancreas necrosis disease (AHPND) emerging as the most prevalent among them. This study can enhance our understanding of infections caused by V. parahaemolyticus and contribute to the development of effective strategies for their prevention and management.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"45"},"PeriodicalIF":4.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Colonization of Serendipita indica enhances resistance against Phoma arachidicola in Arachis hypogaea L.","authors":"Chen Wang, Taswar Ahsan, Ao Ding, Di Han, Jie Gao, Chun-Hao Liang, Si-Tong Du, Yi Wei, Yu-Qian Huang, Shi-Hong Zhang","doi":"10.1007/s11274-025-04263-4","DOIUrl":"10.1007/s11274-025-04263-4","url":null,"abstract":"","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"46"},"PeriodicalIF":4.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable biodegradation of malachite green dye by novel non-pathogenic Pseudomonas aeruginosa ED24.","authors":"Akanksha Pandey, Sachin Kumar, Navneet Bithel, Sandeep Kumar, M Amin Mir","doi":"10.1007/s11274-025-04251-8","DOIUrl":"10.1007/s11274-025-04251-8","url":null,"abstract":"<p><p>Sustainable management of textile industrial wastewater is one of the severe challenges in the current regime. It has been reported that each year huge amount of textile industry discharge especially the dye released into the environment without pre-treatment that adversely affect the human health and plant productivity. In the present study, different bacterial isolates had been isolated from the industrial effluents and investigated for their bioremediation potential against the malachite green (MG) dye, a major pollutant of textile industries. The biochemical and molecular characterization of the bacterial strain showed the resemblance of most potent strain ED24 as Pseudomonas aeruginosa, which showed effective bioremediation potential against the MG dye. During response surface analysis (RSM), best MG degradation conditions have been observed at pH 7.0, 37 °C, 48 h, and 200 mg/L dye concentration, with highest degradation efficiency of 96.56 ± 0.8622 percent. Subsequently, supplementing various carbon and nitrogen sources increases MG decolorization by 1 to 2%, with beef extract (97.23%), sodium nitrate (97.46%), and maltose (98.67%). FT-IR results revealed the disappearance of distinct peaks, namely, 3328.275 cm^-1, 2102.842 cm^-1, 1101.140 cm^-1, and 559.04 cm^-1 from MG, and the formation of major intermediate compounds like leucomalachite green, benzoic acid, diacetamide, benzeneacetic acid, hexyl ester, ethyl 4-acetoxy butanoate, butanoic acid, and 2-methyl in GC-MS analysis of degraded dye sample confirms the biodegradation by bacterial strain ED24. The phytotoxicity studies on mung bean seeds confirmed MG dye toxicity reduction up to 67.53%, 54.16%, and 67.53% in biomass accumulation, root, and shoot lengths, respectively. Also, the microbial toxicity of MG was completely reduced on soil microflora Bacillus flexus, Stenotrophomonas maltophilia, Escherichia coli, Staphylococcus aureus, and Alternaria spp. The dual mitigation, both in microbial and plant systems, indicates the strong remediation potential of P. aeruginosa ED24 to break down MG dye ecologically sustainably.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"44"},"PeriodicalIF":4.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shifts of abundance and community composition of nitrifying microbes along the Changjiang Estuary to the East China Sea.","authors":"Yongkai Chang, Cheng Liu, Zongxiao Zhang, Dengzhou Gao","doi":"10.1007/s11274-025-04259-0","DOIUrl":"10.1007/s11274-025-04259-0","url":null,"abstract":"<p><p>Nitrification, the oxidation of ammonium to nitrate via nitrite, links nitrogen fixation and nitrogen loss processes, playing key roles in coastal nitrogen cycle. However, few studies have simultaneously examined both ammonia-oxidizing and nitrite-oxidizing microbes. This work investigated the abundance and community structure of ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB) using archaeal amoA gene, bacterial amoA gene, and NOB nxrB gene, respectively, through q-PCR and Sanger sequencing along the Changjiang Estuary salinity gradient. Results showed that ammonia oxidizers were dominated by AOB and had higher abundance than NOB. AOA had a higher diversity at high-salinity stations, and AOB diversity decreased along the estuarine salinity gradient. The communities of AOA differed among freshwater, estuarine mixing and seawater zones, indicating a narrow ecological niche. AOB compositions displayed a wide niche, changing from Nitrosomonas-like sequences dominated to Nitrosospira-like sequences dominated along the salinity gradient. The RDA showed that sand and nitrate contents had significant impacts on the AOA community compositions, while the AOB communities were governed by clay and nitrate contents. This research provides insight into the understanding of the niche of ammonia oxidizers in the estuarine zones.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"43"},"PeriodicalIF":4.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed bacterization with siderophore-producing bacteria: a strategy to enhance growth and alkaloid content in Catharanthus roseus.","authors":"Vyoma Mistry, Sapna Chandwani, Natarajan Amaresan, Deepti Kaushik, Ramar Krishnamurthy, Abhishek Sharma","doi":"10.1007/s11274-025-04257-2","DOIUrl":"10.1007/s11274-025-04257-2","url":null,"abstract":"<p><p>Catharanthus roseus is a medicinal plant widely known for producing monoterpenoid indole alkaloids (MIAs), including therapeutic compounds such as vinblastine and vincristine, which are crucial for cancer treatment. However, the naturally low concentration of these alkaloids in plant tissues poses a significant challenge for large-scale production. This study explores the application of siderophore-producing bacteria for seed bacterization of Catharanthus roseus to enhance the production of MIAs, including vindoline, catharanthine, and vinblastine. Utilizing High-Performance Liquid Chromatography (HPLC), we observed a significant increase in the concentration of these alkaloids in bacterized plants compared to controls. FTIR spectra of treated plants showed strong correlations with standard alkaloid mixtures, confirming higher alkaloid accumulation. Our findings demonstrate that bacterial siderophores play a vital role in optimizing iron uptake, which is crucial for secondary metabolite biosynthesis. This research highlights the potential of using microbial biotechnology to improve the yield of valuable pharmaceutical compounds in medicinal plants. Enhancing the biosynthetic pathways of MIAs offers a sustainable and efficient strategy for boosting the production of key therapeutic alkaloids in Catharanthus roseus, paving the way for advanced biotechnological applications in plant-based drug production.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"42"},"PeriodicalIF":4.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications.","authors":"Rine Christopher Reuben, Carmen Torres","doi":"10.1007/s11274-024-04242-1","DOIUrl":"10.1007/s11274-024-04242-1","url":null,"abstract":"<p><p>Mammalian milk contains a variety of complex bioactive and nutritional components and microorganisms. These microorganisms have diverse compositions and functional roles that impact host health and disease pathophysiology, especially mastitis. The advent and use of high throughput omics technologies, including metagenomics, metatranscriptomics, metaproteomics, metametabolomics, as well as culturomics in milk microbiome studies suggest strong relationships between host phenotype and milk microbiome signatures in mastitis. While single omics studies have undoubtedly contributed to our current understanding of milk microbiome and mastitis, they often provide limited information, targeting only a single biological viewpoint which is insufficient to provide system-wide information necessary for elucidating the biological footprints and molecular mechanisms driving mastitis and milk microbiome dysbiosis. Therefore, integrating a multi-omics approach in milk microbiome research could generate new knowledge, improve the current understanding of the functional and structural signatures of the milk ecosystem, and provide insights for sustainable mastitis control and microbiome management.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"41"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioleaching of lanthanum from nickel metal hydride dry battery using siderophores produced by Pseudomonas sp.","authors":"Amany S Hegazy, Hoda M Soliman, Amr M Mowafy, Attiya H Mohamedin","doi":"10.1007/s11274-025-04250-9","DOIUrl":"10.1007/s11274-025-04250-9","url":null,"abstract":"<p><p>There is still much to be learned about the properties of siderophores and their applications. This study was designed to characterize and optimize the production of the siderophore produced by a marine bacterium Pseudomonas sp. strain ASA235 and then evaluate their use in bioleaching of rare earth elements (REEs) from spent Nickel-metal hydride (NiMH) batteries. The results of both Tetrazolium and Arnow's tests indicated that the test organism produces a mixed-type siderophore of pyoverdine family, a result that was confirmed by FT-IR and MALDI-TOFF analyses. Optimization of pH, temperature, incubation period, and iron concentration for siderophore production led to a noticeable shift from 44.5% up to 91% siderophore unit when the test bacterium was incubated at 28 °C and pH 7 after 72 h in the absence of iron. The purified siderophore showed the ability to bleach about 14.8% of lanthanum from the anode of the NiMH battery along with other elements, although in lower amounts. This data put siderophores in distinct focus for further prospective studies intending the bioleaching of such precious elements. The scaling up of this process and optimization would make a big difference in such a green bioleaching strategy, allowing us to recover such precious elements in an environmentally friendly way.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"39"},"PeriodicalIF":4.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}