World journal of microbiology & biotechnology最新文献_第9页

Algal-bacterial bioremediation of cyanide-containing wastewater in a continuous stirred photobioreactor. 连续搅拌光生物反应器中含氰废水的藻-细菌生物修复。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-09 DOI: 10.1007/s11274-024-04230-5

Mona F AbdelMageed, Marwa T ElRakaiby

{"title":"Algal-bacterial bioremediation of cyanide-containing wastewater in a continuous stirred photobioreactor.","authors":"Mona F AbdelMageed, Marwa T ElRakaiby","doi":"10.1007/s11274-024-04230-5","DOIUrl":"10.1007/s11274-024-04230-5","url":null,"abstract":"This study reports the isolation and characterization of highly resistant bacterial and microalgal strains from an Egyptian wastewater treatment station to cyanide-containing compounds. The bacterial strain was identified as Bacillus licheniformis by 16S rRNA gene sequencing. The isolate removed up to 1 g L-1 potassium cyanide, 3 g L-1 benzonitrile, and 1 g L-1 sodium salicylate when incubated as 10% v/v in MSM at 30 ℃. However, it failed to degrade potassium thiocyanate at all tested concentrations. The microalgal isolate was identified by electron microscopy as a strain of Chlorella spp.. Algal toxicity was tested by incubating the microalgae as 6% v/v in MSM containing 2 g L- 1 NaHCO3 with increasing concentrations of the pollutants. Results showed that 0.05 g L-1 KCN, 1.5 g L-1 benzonitrile, 5 g L -1 KSCN, and 5 g L-1 sodium salicylate inhibited 93%, 96%, 75%, and 21% of algal growth, respectively. In a continuous stirred photobioreactor, the bacterial-microalgal microcosm detoxified synthetic wastewater containing 0.2 g L-1 KCN, 0.1 g L-1 benzonitrile, and 0.5 g L-1 sodium salicylate in 3.5 days of hydraulic retention time. System failure was recorded when the KCN concentration was increased to 0.25 g L-1. The effluent had no inhibitory effect on the germination of Lepidium sativum seeds in phytotoxicity testing. Temperature, pH, and chitosan effects were assessed on the algal/bacterial settleability. Statistical analysis showed no significant difference between the tested parameters. The microcosm represents a potential candidate for the treatment of industrial wastewater containing cyanide compounds.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"26"},"PeriodicalIF":4.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11711256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the dual role of biocolonization: a case study on the deterioration and preservation of sandstone monuments in Leshan Giant Buddha, China.

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-07 DOI: 10.1007/s11274-024-04237-y

Chengshuai Zhu, Bowen Wang, Zhengdong Liu, Yifei Guo, Linting Zheng, Bingjian Zhang, Yulan Hu

{"title":"Unveiling the dual role of biocolonization: a case study on the deterioration and preservation of sandstone monuments in Leshan Giant Buddha, China.","authors":"Chengshuai Zhu, Bowen Wang, Zhengdong Liu, Yifei Guo, Linting Zheng, Bingjian Zhang, Yulan Hu","doi":"10.1007/s11274-024-04237-y","DOIUrl":"https://doi.org/10.1007/s11274-024-04237-y","url":null,"abstract":"For large, open-air lithic cultural heritage, colonization is an inevitable process. This study examines the dual impact of colonization on the Leshan Giant Buddha's sandstone monuments, focusing on both biodeterioration and protection. Over three years, we conducted field surveys and monitored biocrusts (bryophytes, lichens, and biofilms) on these monuments, observing significant biodeterioration primarily due to mechanical exfoliation and acid corrosion. To assess the potential of biota in conserving sandstone monuments, we replicated biotic crust colonization on laboratory sandstone samples. In the laboratory, we observed biomineral formation, primarily calcium carbonate, on the rock specimen surfaces after two years. Comparative analysis with control samples showed that biocolonization notably changed the rock's hydrophobicity, surface hardness, and weight loss, indicating a protective effect. Further analyses using XRD, SEM-EDS, and transcriptomics identified carbonic anhydrase as a key enzyme in the biomineralization process. Our findings suggest that under controlled conditions, biocolonization can be steered towards bioprotection, offering new strategies for the in-situ preservation of open-air lithic cultural heritage.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"25"},"PeriodicalIF":4.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Algae- and bacteria-based biodegradation of phthalic acid esters towards the sustainable green solution. 以藻类和细菌为基础的邻苯二甲酸酯生物降解的可持续绿色解决方案。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-07 DOI: 10.1007/s11274-024-04243-0

Veghar Amirian, Mohammad Russel, Zetty Norhana Balia Yusof, Jit Ern Chen, Ali Movafeghi, Morteza Kosari-Nasab, Dayong Zhang, Ewa Szpyrka

{"title":"Algae- and bacteria-based biodegradation of phthalic acid esters towards the sustainable green solution.","authors":"Veghar Amirian, Mohammad Russel, Zetty Norhana Balia Yusof, Jit Ern Chen, Ali Movafeghi, Morteza Kosari-Nasab, Dayong Zhang, Ewa Szpyrka","doi":"10.1007/s11274-024-04243-0","DOIUrl":"10.1007/s11274-024-04243-0","url":null,"abstract":"Phthalic acid esters are widely used worldwide as plasticizers. The high consumption of phthalates in China makes it the world's largest plasticizer market. The lack of phthalic acid ester's chemical bonding with the polymer matrix facilitates their detachment from plastic products and subsequent release into the environment and causes serious threats to the health of living organisms. Thus, environmentally friendly and sustainable solutions for their removal are urgently needed. In this context, both natural and engineered bacterial and algal communities have played a crucial role in the degradation of various phthalic acid esters present in water and soil. When algae-bacteria co-culture is compared to a singular algae or bacteria system, this symbiotic system shows superior performance in the removal of dibutyl phthalates and diethyl phthalates from synthetic wastewater. This review provides an optimistic outlook for co-culture systems by in-depth examining single microorganisms, namely bacteria and algae, as well as algae-bacterial consortiums for phthalates degradation, which will draw attention to species co-existence for the removal of various pollutants from the environment. In addition, further development and research, particularly on the mechanisms, genes involved in the degradation of phthalic acid esters, and interactions between bacterial and algal species, will lead to the discovery of more adaptable species as well as the production of targeted species to address the environmental pollution crisis and provide a green, efficient, and sustainable approach to environmental protection. Discrepancies in knowledge and potential avenues for exploration will enhance the existing body of literature, enabling researchers to investigate this field more comprehensively.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 2","pages":"24"},"PeriodicalIF":4.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Bacillus licheniformis DW2 for ectoine production. 地衣芽孢杆菌DW2的代谢工程研究。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2025-01-02 DOI: 10.1007/s11274-024-04238-x

Xujie Li, Aoying Dong, Junru Yang, Jiang Zhu, Yangyang Zhan, Xin Ma, Dongbo Cai, Shouwen Chen

{"title":"Metabolic engineering of Bacillus licheniformis DW2 for ectoine production.","authors":"Xujie Li, Aoying Dong, Junru Yang, Jiang Zhu, Yangyang Zhan, Xin Ma, Dongbo Cai, Shouwen Chen","doi":"10.1007/s11274-024-04238-x","DOIUrl":"10.1007/s11274-024-04238-x","url":null,"abstract":"Ectoine is a high-value protective agent with extensive applications in the fields of fine chemicals and biopharmaceuticals, and it is naturally synthesized by Halomonas in extreme environment, however, the current production level cannot meet the growing market demand. In this study, we aimed to develop an efficient and environmentally friendly ectoine production process using Bacillus licheniformis as the host organism. Through introducing ectoine synthetase gene cluster ectABC from Halomonas elongate, as well as optimizing ectABCHs expression by promoter and 5'-UTR optimization, ectoine titer was increased to 0.55 g/L. Furthermore, subsequent introduction of exogenous phosphoenolpyruvate carboxylase PPCEC and down-regulated expression of phosphoenolpyruvate carboxykinase PCK optimized the carbon flux through C4 anaplerotic pathway, and further benefited ectoine synthesis. Furthermore, the carbon flux towards aspartic acid accumulation was increased through optimization of glyoxylate and TCA cycles, accompanied with introducing lysCT311ICg and asdCg, and blocking by-products pathways, ectoine titer produced by B. licheniformis ECT12 was 2.00 g/L. Moreover, NADPH supply was enhanced by overexpression of exogenous NADH kinase Pos5Sc, and ectoine transportation was improved by introducing compatible solute transporter ProP from Escherichia coli, and the resulting B. licheniformis ECT14 was able to produce 2.60 g/L ectoine. Last but not the least, the ectoine yield of 3.29 g/L was attained in a 5-L fermenter. Taken together, this study not only established B. licheniformis as a framework for sustainable production of ectoine, but also paved the way for achieving the industrial production of ectoine and aspartic acid derivatives in the future.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"23"},"PeriodicalIF":4.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological advances in plant growth-promoting rhizobacteria for sustainable agriculture. 促进植物生长的根瘤菌在可持续农业中的生物技术进展。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-30 DOI: 10.1007/s11274-024-04231-4

Leandris Argentel-Martínez, Ofelda Peñuelas-Rubio, Angélica Herrera-Sepúlveda, Jorge González-Aguilera, Surya Sudheer, Linu M Salim, Sunaina Lal, Chittethu Kunjan Pradeep, Aurelio Ortiz, Estibaliz Sansinenea, Sandamali Harshani Kumari Hathurusinghe, Jae-Ho Shin, Olubukola Oluranti Babalola, Ugur Azizoglu

{"title":"Biotechnological advances in plant growth-promoting rhizobacteria for sustainable agriculture.","authors":"Leandris Argentel-Martínez, Ofelda Peñuelas-Rubio, Angélica Herrera-Sepúlveda, Jorge González-Aguilera, Surya Sudheer, Linu M Salim, Sunaina Lal, Chittethu Kunjan Pradeep, Aurelio Ortiz, Estibaliz Sansinenea, Sandamali Harshani Kumari Hathurusinghe, Jae-Ho Shin, Olubukola Oluranti Babalola, Ugur Azizoglu","doi":"10.1007/s11274-024-04231-4","DOIUrl":"10.1007/s11274-024-04231-4","url":null,"abstract":"The rhizosphere, the soil zone surrounding plant roots, serves as a reservoir for numerous beneficial microorganisms that enhance plant productivity and crop yield, with substantial potential for application as biofertilizers. These microbes play critical roles in ecological processes such as nutrient recycling, organic matter decomposition, and mineralization. Plant growth-promoting rhizobacteria (PGPR) represent a promising tool for sustainable agriculture, enabling green management of crop health and growth, being eco-friendly alternatives to replace chemical fertilizers and pesticides. In this sense, biotechnological advancements respecting genomics and gene editing have been crucial to develop microbiome engineering which is pivotal in developing microbial consortia to improve crop production. Genome mining, which involves comprehensive analysis of the entire genome sequence data of PGPR, is crucial for identifying genes encoding valuable bacterial enzymes and metabolites. The CRISPR-Cas system, a cutting-edge genome-editing technology, has shown significant promise in beneficial microbial species. Advances in genetic engineering, particularly CRISPR-Cas, have markedly enhanced grain output, plant biomass, resistance to pests, and the sensory and nutritional quality of crops. There has been a great advance about the use of PGPR in important crops; however, there is a need to go further studying synthetic microbial communities, microbiome engineering, and gene editing approaches in field trials. This review focuses on future research directions involving several factors and topics around the use of PGPR putting special emphasis on biotechnological advances.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"21"},"PeriodicalIF":4.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The conserved protein DopA is required for growth, drug tolerance and virulence in Aspergillus fumigatus. 保守蛋白多巴是烟曲霉生长、耐药和毒力所必需的。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-30 DOI: 10.1007/s11274-024-04234-1

Qian Wei, Cong He, Xinyuan Peng, Bingyi An, Min Peng, Xiaoyu Wang, Chen Zhang, Ling Lu, Hong Sang, Qingtao Kong

{"title":"The conserved protein DopA is required for growth, drug tolerance and virulence in Aspergillus fumigatus.","authors":"Qian Wei, Cong He, Xinyuan Peng, Bingyi An, Min Peng, Xiaoyu Wang, Chen Zhang, Ling Lu, Hong Sang, Qingtao Kong","doi":"10.1007/s11274-024-04234-1","DOIUrl":"10.1007/s11274-024-04234-1","url":null,"abstract":"The majority of Aspergillus fumigatus reproduction occurs asexually, with large numbers of conidiophores producing small hydrophobic conidia dispersed aerially. When healthy hosts inhale conidia, the mucosal cilia and phagocytosis by the innate immune system can remove them. However, in immunocompromised hosts, the conidia are not removed, which allows them to germinate, forming mycelium that invades host tissues and causes disease. Previously we isolated a white A. fumigatus A1j strain incapable of producing conidia and screened several genes (including dopA) with significant expression differences and mutant loci in A1j. DopA homologous proteins in other species have been partially studied and are known to participate in various membrane transport-related cellular functions. Defects in these proteins in Saccharomyces cerevisiae, Caenorhabditis elegans, and Aspergillus nidulans result in defective cell morphology and abnormal growth. In this study, we observed reduced conidia production and abnormal development of spore-producing structures in the A. fumigatus dopA null strain, compared to parental strain, and demonstrated that dopA also modulates stress response and virulence of A. fumigatus.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"19"},"PeriodicalIF":4.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the impact of arsenic on symbiotic and free-living PGPB: plant growth promoting traits, bacterial compatibility and adhesion on soybean seed. 评估砷对共生和游离PGPB的影响：大豆种子的植物生长促进性状、细菌相容性和粘附性。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-30 DOI: 10.1007/s11274-024-04233-2

Romina Del Pilar Pramparo, Mariana Elisa Vezza, Ana Laura Wevar Oller, Melina Andrea Talano, Elizabeth Agostini

{"title":"Assessing the impact of arsenic on symbiotic and free-living PGPB: plant growth promoting traits, bacterial compatibility and adhesion on soybean seed.","authors":"Romina Del Pilar Pramparo, Mariana Elisa Vezza, Ana Laura Wevar Oller, Melina Andrea Talano, Elizabeth Agostini","doi":"10.1007/s11274-024-04233-2","DOIUrl":"10.1007/s11274-024-04233-2","url":null,"abstract":"Arsenic (As) contamination in agricultural groundwater and soil is a significant economic and health problem worldwide. It inhibits soybean (Glycine max (L.) Merr.) nodulation and biological nitrogen fixation in symbiosis with Bradyrhizobium japonicum E109 (E109), a commonly used rhizobial strain for commercial biofertiliser formulation in Argentina. In the context of sustainable and climate-smart agriculture promoted by FAO, co-inoculating legumes with As-tolerant plant growth-promoting bacteria (PGPB) is suggested as a superior alternative to single inoculation. This study aimed to evaluate the impact of As on plant growth-promoting (PGP) traits -siderophore and indole acetic acid production, phosphate solubilisation, diazotrophic activity and hydrolytic enzymes activity- in E109 and three other PGPB strains: Pseudomonas sp. AW4 (AW4), Bacillus pumilus SF5 (SF5) and Bacillus toyonensis SFC 500-1E (Bt). In addition, bacterial compatibility and adhesion on soybean seed were evaluated. Arsenic significantly reduced PGP traits of E109 even at low concentrations, AW4's traits remained unchanged, while those of SF5 and Bt traits were affected (positively or negatively) only at the highest concentrations tested (500 µM arsenate, 250 µM arsenite). All PGPB strains were compatible with E109 under both control and As-stress conditions. Soybean seed adhesion was reduced for E109, only under As stress. Findings suggest that the effect of As on PGP traits is highly strain-dependent and influenced by As concentration and speciation. AW4, SF5, and Bt strains show promise for co-inoculation with E109 in soybean cultivation.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"20"},"PeriodicalIF":4.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multilocus sequencing analysis of the rhizobial symbionts isolated from Acacia salicina (Lindl.) grown in different regions in Tunisia reveals putative novel Bradyrhizobium species. 对生长在突尼斯不同地区的金合欢（Acacia salicina）中分离的根瘤菌的多位点测序分析揭示了假定的新型慢生根瘤菌。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-30 DOI: 10.1007/s11274-024-04236-z

Mokhtar Rejili, Besma Bouznif, Mohamed Ali Benabderrahim, Mohamed Mars

{"title":"Multilocus sequencing analysis of the rhizobial symbionts isolated from Acacia salicina (Lindl.) grown in different regions in Tunisia reveals putative novel Bradyrhizobium species.","authors":"Mokhtar Rejili, Besma Bouznif, Mohamed Ali Benabderrahim, Mohamed Mars","doi":"10.1007/s11274-024-04236-z","DOIUrl":"10.1007/s11274-024-04236-z","url":null,"abstract":"In this study, we investigated various chromosomal and symbiotic markers in 40 bacterial strains that nodulate an invasive alien plant, Acacia salicina Lindl. in Tunisia. Our findings showed that the native rhizobia associated to A. salicina are grouped into eight distinct RAPD electrophoretic types (RETs) (genotypes). Sequence analyses of rrs gene and three housekeeping genes (recA, rpoB and glnII) assigned sixteen isolates to three putative new lineages within the genus Bradyrhizobium. Seven strains clustered with B. rifense CTAW71T with a 91% bootstrap support, five strains grouped with B. niftali CNPSo3448T with a very low bootstrap support (60%), and four strains formed a group phylogenetically related with B. shewense ERR11T and B. centrosematis A9T. Based on nodC phylogeny and cross inoculation tests, the 16 strains are clustered within symbiovar retamae (six strains) and cyanophyllae (ten strains). Moreover, we showed by the first time in this work that the type strains B. diversitatis CNPSo4019T and B. xenonodulans 14ABT, which nodulated soybean and A. dealbata respectively, belong to the symbiovar cyanophyllae according to the results of the nodC gene analysis.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"22"},"PeriodicalIF":4.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trans-2-hexenal reduces postharvest mango stem-end rot by oxidative damage to Neofusicoccum parvum cell membranes. 反式-2-己烯醛通过氧化损伤芒果幼膜来减少采后芒果茎端腐病。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-26 DOI: 10.1007/s11274-024-04235-0

Xiaoli Tan, Xiaobing Jiang, Xiumei Chen, Okwong Oketch Reymick, Chen Zhu, Nengguo Tao

{"title":"Trans-2-hexenal reduces postharvest mango stem-end rot by oxidative damage to Neofusicoccum parvum cell membranes.","authors":"Xiaoli Tan, Xiaobing Jiang, Xiumei Chen, Okwong Oketch Reymick, Chen Zhu, Nengguo Tao","doi":"10.1007/s11274-024-04235-0","DOIUrl":"10.1007/s11274-024-04235-0","url":null,"abstract":"Neofusicoccum parvum is one of the most hazardous pathogens causing mango fruit decay. The present study utilized trans-2-hexenal (TH), a typical antifungal component of plant essential oils (EOs), to control N. parvum both in vivo and in vitro, and attempted to explore the mechanisms involved. The findings showed that at concentrations greater than 0.4 µL/mL, TH exhibited exceptional antifungal activity against N. parvum in vitro. TH application led to the disruption of the structural integrity of both cell walls and cell membranes, with a particular impact on the latter, as evidenced by the dramatically increased propidium iodide level, as well as reduced total lipids and ergosterol content. Further DCFH-DA staining experiments showed that TH induced mycelial reactive oxygen species (ROS) accumulation, which exacerbated cell membrane lipid peroxidation. For easier application of TH, we fabricated aerogel-loaded TH (ALTH) materials, which demonstrated excellent antifungal activity in vitro. Infestation studies on fruits demonstrated that ALTH mitigated mango stem-end rot in a dose-dependent fashion, with a concentration of 40 µL/L showing efficacy comparable to the conventional fungicide prochloraz, while maintaining fruit quality. In light of these results, TH works by inducing ROS buildup and oxidative damage to the cell membrane of N. parvum, and is a particularly promising preservative for preventing postharvest infections in mangoes.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"17"},"PeriodicalIF":4.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of bacterial quorum sensing in plant growth promotion. 细菌群体感应在植物生长促进中的作用。

IF 4 3区生物学

World journal of microbiology & biotechnology Pub Date : 2024-12-26 DOI: 10.1007/s11274-024-04232-3

Aparna Anil Singh, Anil Kumar Singh

{"title":"Role of bacterial quorum sensing in plant growth promotion.","authors":"Aparna Anil Singh, Anil Kumar Singh","doi":"10.1007/s11274-024-04232-3","DOIUrl":"10.1007/s11274-024-04232-3","url":null,"abstract":"Quorum sensing (QS) also known as bacterial cell-cell communication or bacterial crosstalk is a phenomenon regulating various bacterial traits that can affect plant growth and defence. Similarities in the structure of root exudates and bacterial signalling molecules have tremendous implications governing the plant heath. The rhizosphere ecosystem being an excellent example of plant-microbe and microbe-microbe interactions harbours a variety of microorganisms exhibiting quorum sensing. Phytochemicals present in plant root exudates and QS signal molecules as well as volatile organic compounds (VOCs) produced by microorganisms work in coordination to establish intra- and inter-species communications. Interestingly, a number of plant growth promoting rhziobacterial (PGPR) activities like effective/enhanced root colonization, nutrient uptake, nodulation, nitrogen fixation, production of plant hormones, antimicrobial compounds and induction of plant defences can be attributed directly or indirectly to their quorum sensing and quenching abilities. Although not completely understood, root development, stress tolerance and defence against phytopathogens are some of the implications of such abilities which might prove beneficial for sustainable agriculture. Deciphering the mechanism of these interactions would be instrumental in improving crop health. Plant beneficial microorganisms employing QS and QS inhibition (QSI) strategies have been discussed in this review.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 1","pages":"18"},"PeriodicalIF":4.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0