Microbial populations under fluoride stress: a metagenomic exploration from Indian soil.

IF 4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Krishnendu Pramanik, Arup Sen, Subrata Dutta, Gouranga Sundar Mandal, Bappa Paramanik, Arpita Das, Nitin Chatterjee, Ankit Kumar Ghorai, Md Nasim Ali
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引用次数: 0

Abstract

Fluoride exposure, even at a low concentration, significantly impairs crop growth and productivity by inhibiting metabolic enzymes and disrupting photosynthesis. Addressing this challenge, microbial de-fluoridation emerges as a vital strategy to improve soil health, enhance crop growth, and ensure agricultural sustainability. This study analyzed topsoil samples (0-0.2 m depth) from rice fields in three blocks of Purulia district, West Bengal-Arsha, Jhalda-I, and Joypur. Fluoride content in the samples ranged from 58.76 ± 0.76 mg/kg to 282.9 ± 4.9 mg/kg (total) and 1.57 ± 0.02 mg/kg to 2.97 ± 0.03 mg/kg (available). The metagenomic analysis of the collected soil samples revealed diverse microbial communities comprising archaea, bacteria, fungi, and viruses, with Actinobacteria (phylum), Hyphomicrobiales (order), and Nocardioidaceae (family) being the dominant prokaryotes. Arsha soil with comparatively low fluoride contamination exhibited the highest microbial diversity (11,891 taxa), followed by Joypur (11,528 taxa) and Jhalda-I (11,358 taxa), with Arsha showing nearly double the unique microbial taxa compared to the other locations. Clusters of orthologous groups of proteins functional analysis identified 60,898 genes in Arsha, 63,403 genes in Jhalda-I, and 73,334 genes in Joypur, while Kyoto encyclopedia of genes and genomes analysis revealed 9,385, 9,104, and 10,633 genes, respectively. Key genes associated with fluoride metabolism-inorganic pyrophosphatase, divalent metal cation transporter mntH, and putative fluoride ion transporter crcB-were abundant across all sites, highlighting the influence of fluoride on microbial community structure. This study provides the first comprehensive report on soil microbial communities in fluoride-rich areas, highlighting the potential of native fluoride-tolerant microbes to mitigate fluoride toxicity in agricultural soils and offer sustainable, microbe-based solutions to fluoride contamination.

氟胁迫下的微生物种群:来自印度土壤的宏基因组探索。
接触氟化物,即使浓度很低,也会抑制代谢酶和破坏光合作用,从而严重损害作物生长和生产力。为了应对这一挑战,微生物除氟成为改善土壤健康、促进作物生长和确保农业可持续性的一项重要战略。本研究分析了Purulia区、West Bengal-Arsha、Jhalda-I和Joypur三个街区稻田的表土样本(0-0.2 m深度)。样品中氟化物含量为58.76±0.76 mg/kg至282.9±4.9 mg/kg(总)和1.57±0.02 mg/kg至2.97±0.03 mg/kg(可获得)。对收集的土壤样品进行宏基因组分析,发现微生物群落多样,包括古细菌、细菌、真菌和病毒,其中放线菌门、菌丝菌目和诺卡菌科是主要的原核生物。氟污染较低的Arsha土壤微生物多样性最高(11,891个类群),其次是Joypur(11,528个类群)和Jhalda-I(11,358个类群),其中Arsha的微生物类群几乎是其他地区的两倍。同源蛋白群功能分析在Arsha中鉴定出60,898个基因,在Jhalda-I中鉴定出63,403个基因,在Joypur中鉴定出73,334个基因,而京都基因和基因组百科全书分析分别鉴定出9,385个,9,104个和10,633个基因。与氟代谢相关的关键基因——无机焦磷酸酶、二价金属阳离子转运体mntH和推定的氟离子转运体crcb——在所有位点都很丰富,这突出了氟对微生物群落结构的影响。这项研究提供了第一份关于富氟地区土壤微生物群落的综合报告,强调了天然耐氟微生物在减轻农业土壤氟毒性方面的潜力,并提供了可持续的、基于微生物的氟化物污染解决方案。
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来源期刊
World journal of microbiology & biotechnology
World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.30
自引率
2.40%
发文量
257
审稿时长
2.5 months
期刊介绍: World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.
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