Microbial diversity analysis of municipal solid waste landfills soils of Delhi (NCR) and plastic dump sites of Uttar Pradesh region of India and their function prediction for plastic degrading enzymes.
IF 4.2 3区 生物学Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0
Abstract
Municipal solid waste landfills are rich in plastic waste, fostering a unique microbial ecosystem distinct from natural habitats. This study aimed to investigate the microbial diversity at four landfill and plastic dump sites using a 16S rDNA metataxanomics approach, and to predict their potential for plastic degradation. Environmental DNA was isolated and analyzed through Illumina MiSeq sequencing. The results revealed a dominant presence of Pseudomonadota (47%) across all sites, with key genera including Streptomyces, Galbibacter, Alcanivorax, and OM190. The most abundant species were unclassified Chloroflexi bacterium, Galbibacter marinus, Gracilimonas amylolytica, and Teredinibacter sp. Alpha diversity analysis showed the highest species richness in plastic dump site in Utrathia (Lucknow), followed by Ghazipur landfill Site (Delhi), with low evenness across the bacterial communities. Beta diversity analysis, using Bray-Curtis and PCA, indicated distinct microbial profiles for each site. The PICRUSt analysis identified 402 genes related to nine enzyme categories involved in plastic degradation. Pearson correlation network analysis of top 1% genera highlighted positive associations between genera like Galbibacter, Alcanivorax, Thioalkalimicrobium, Idiomarina and Pseudomonas with peroxygenase enzyme. This study underscores the microbial diversity and functional potential of landfill microbes in plastic degradation, contributing to our understanding of the plastisphere microbiome in landfill environments.
期刊介绍:
World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology.
Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions.
Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories:
· Virology
· Simple isolation of microbes from local sources
· Simple descriptions of an environment or reports on a procedure
· Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism
· Data reporting on host response to microbes
· Optimization of a procedure
· Description of the biological effects of not fully identified compounds or undefined extracts of natural origin
· Data on not fully purified enzymes or procedures in which they are applied
All articles published in the Journal are independently refereed.