Microbial diversity analysis of municipal solid waste landfills soils of Delhi (NCR) and plastic dump sites of Uttar Pradesh region of India and their function prediction for plastic degrading enzymes.

IF 4.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Viral Kumar Tiwari, Akanksha Vishwakarma, Digvijay Verma, Monica Sharma
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Abstract

Municipal solid waste landfills are rich in plastic waste, fostering a unique microbial ecosystem distinct from natural habitats. This study aimed to investigate the microbial diversity at four landfill and plastic dump sites using a 16S rDNA metataxanomics approach, and to predict their potential for plastic degradation. Environmental DNA was isolated and analyzed through Illumina MiSeq sequencing. The results revealed a dominant presence of Pseudomonadota (47%) across all sites, with key genera including Streptomyces, Galbibacter, Alcanivorax, and OM190. The most abundant species were unclassified Chloroflexi bacterium, Galbibacter marinus, Gracilimonas amylolytica, and Teredinibacter sp. Alpha diversity analysis showed the highest species richness in plastic dump site in Utrathia (Lucknow), followed by Ghazipur landfill Site (Delhi), with low evenness across the bacterial communities. Beta diversity analysis, using Bray-Curtis and PCA, indicated distinct microbial profiles for each site. The PICRUSt analysis identified 402 genes related to nine enzyme categories involved in plastic degradation. Pearson correlation network analysis of top 1% genera highlighted positive associations between genera like Galbibacter, Alcanivorax, Thioalkalimicrobium, Idiomarina and Pseudomonas with peroxygenase enzyme. This study underscores the microbial diversity and functional potential of landfill microbes in plastic degradation, contributing to our understanding of the plastisphere microbiome in landfill environments.

德里城市生活垃圾填埋场和印度北方邦塑料垃圾场土壤微生物多样性分析及其对塑料降解酶的功能预测
城市固体垃圾填埋场中含有丰富的塑料垃圾,形成了不同于自然栖息地的独特微生物生态系统。本研究旨在利用16S rDNA元类群学方法研究四个垃圾填埋场和塑料垃圾场的微生物多样性,并预测其降解塑料的潜力。分离环境DNA,通过Illumina MiSeq测序进行分析。结果显示假单胞菌属(47%)在所有位点均占主导地位,主要属包括链霉菌属、Galbibacter属、Alcanivorax属和OM190属。物种丰富度最高的是未分类的Chloroflexi细菌、Galbibacter marinus、Gracilimonas amylolytica和Teredinibacter sp. Alpha多样性分析显示,Utrathia (Lucknow)垃圾场的物种丰富度最高,其次是Ghazipur (Delhi)垃圾场,各细菌群落的均匀度较低。利用Bray-Curtis和PCA进行的Beta多样性分析表明,每个站点的微生物特征不同。PICRUSt分析鉴定出402个与塑料降解相关的9类酶相关的基因。Pearson相关网络分析显示,前1%属中Galbibacter、Alcanivorax、thiioalkali - icroum、Idiomarina和Pseudomonas等属与过氧酶呈正相关。本研究强调了垃圾填埋场微生物在塑料降解中的微生物多样性和功能潜力,有助于我们了解垃圾填埋场环境中的塑料圈微生物群。
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来源期刊
World journal of microbiology & biotechnology
World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.30
自引率
2.40%
发文量
257
审稿时长
2.5 months
期刊介绍: World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.
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