{"title":"Plant growth-promoting Burkholderia Sp. ZF6 enhances Brassica juncea survival in Cd/Zn contaminated soils via heavy metal tolerance genes.","authors":"Soo Yeon Lee, Kyung-Suk Cho","doi":"10.1007/s11274-025-04429-0","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, we focused on Burkholderia sp. ZF6, a promising plant growth-promoting bacterium (PGPB) known for its robust heavy metal tolerance, enduring 5 mM cadmium (Cd) and 50 mM zinc (Zn), and carrying the tolerance genes cadA and zntA. ZF6 showed outstanding plant growth-promoting (PGP) abilities, including nitrogen fixation (OD<sub>630</sub> = 0.331 ± 0.012), over 50% DPPH radical scavenging activity, high levels of indole-3-acetic acid (IAA) production, phosphatase activity, and siderophore production. For the experimental trials, ZF6 was used to inoculate Indian mustard (Brassica juncea) seeds in multi-metal-contaminated soil (smelter soil), resulting in a 1.4-fold increase in seed germination. Subsequent pot experiments with soil co-contaminated with 100 mg Cd·kg<sup>- 1</sup> and 2,000 mg Zn·kg<sup>- 1</sup> demonstrated a 1.5- and 1.6-fold increase in shoot and root lengths, respectively, a 2.1-fold boost in vigor index, and a 1.1- to 1.2-fold rise in plant biomass. Over the experiment period, the relative abundance of cadA and zntA genes increased by 1.6- and 18.4-fold, respectively. These findings demonstrate the potential of Burkholderia sp. ZF6 as a biofertilizer that enhances heavy metal tolerance and promotes Indian mustard growth in highly contaminated soil while improving overall soil PGP activity.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 6","pages":"191"},"PeriodicalIF":4.2000,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"World journal of microbiology & biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11274-025-04429-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, we focused on Burkholderia sp. ZF6, a promising plant growth-promoting bacterium (PGPB) known for its robust heavy metal tolerance, enduring 5 mM cadmium (Cd) and 50 mM zinc (Zn), and carrying the tolerance genes cadA and zntA. ZF6 showed outstanding plant growth-promoting (PGP) abilities, including nitrogen fixation (OD630 = 0.331 ± 0.012), over 50% DPPH radical scavenging activity, high levels of indole-3-acetic acid (IAA) production, phosphatase activity, and siderophore production. For the experimental trials, ZF6 was used to inoculate Indian mustard (Brassica juncea) seeds in multi-metal-contaminated soil (smelter soil), resulting in a 1.4-fold increase in seed germination. Subsequent pot experiments with soil co-contaminated with 100 mg Cd·kg- 1 and 2,000 mg Zn·kg- 1 demonstrated a 1.5- and 1.6-fold increase in shoot and root lengths, respectively, a 2.1-fold boost in vigor index, and a 1.1- to 1.2-fold rise in plant biomass. Over the experiment period, the relative abundance of cadA and zntA genes increased by 1.6- and 18.4-fold, respectively. These findings demonstrate the potential of Burkholderia sp. ZF6 as a biofertilizer that enhances heavy metal tolerance and promotes Indian mustard growth in highly contaminated soil while improving overall soil PGP activity.
期刊介绍:
World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology.
Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions.
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