Plant growth-promoting Burkholderia Sp. ZF6 enhances Brassica juncea survival in Cd/Zn contaminated soils via heavy metal tolerance genes.

IF 4.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Soo Yeon Lee, Kyung-Suk Cho
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Abstract

In this study, we focused on Burkholderia sp. ZF6, a promising plant growth-promoting bacterium (PGPB) known for its robust heavy metal tolerance, enduring 5 mM cadmium (Cd) and 50 mM zinc (Zn), and carrying the tolerance genes cadA and zntA. ZF6 showed outstanding plant growth-promoting (PGP) abilities, including nitrogen fixation (OD630 = 0.331 ± 0.012), over 50% DPPH radical scavenging activity, high levels of indole-3-acetic acid (IAA) production, phosphatase activity, and siderophore production. For the experimental trials, ZF6 was used to inoculate Indian mustard (Brassica juncea) seeds in multi-metal-contaminated soil (smelter soil), resulting in a 1.4-fold increase in seed germination. Subsequent pot experiments with soil co-contaminated with 100 mg Cd·kg- 1 and 2,000 mg Zn·kg- 1 demonstrated a 1.5- and 1.6-fold increase in shoot and root lengths, respectively, a 2.1-fold boost in vigor index, and a 1.1- to 1.2-fold rise in plant biomass. Over the experiment period, the relative abundance of cadA and zntA genes increased by 1.6- and 18.4-fold, respectively. These findings demonstrate the potential of Burkholderia sp. ZF6 as a biofertilizer that enhances heavy metal tolerance and promotes Indian mustard growth in highly contaminated soil while improving overall soil PGP activity.

植物促生菌Burkholderia Sp. ZF6通过重金属耐受基因提高芥菜在Cd/Zn污染土壤中的生存能力。
在这项研究中,我们重点研究了Burkholderia sp. ZF6,这是一种有前景的植物生长促进细菌(PGPB),以其强大的重金属耐受性而闻名,耐受5毫米镉(Cd)和50毫米锌(Zn),并携带耐受基因cadA和zntA。ZF6具有良好的植物生长促进能力,包括固氮(OD630 = 0.331±0.012),超过50%的DPPH自由基清除能力,高水平的吲哚-3-乙酸(IAA)生成,磷酸酶活性和铁载体生成。在试验试验中,用ZF6在多金属污染土壤(冶炼厂土壤)中接种芥菜(Brassica juncea)种子,使种子萌发率提高1.4倍。在随后的盆栽试验中,土壤同时受100 mg Cd·kg- 1和2000 mg Zn·kg- 1污染,茎长和根长分别增加1.5倍和1.6倍,活力指数增加2.1倍,生物量增加1.1至1.2倍。在试验期间,cadA和zntA基因的相对丰度分别增加了1.6倍和18.4倍。这些研究结果表明,伯克霍尔德菌ZF6作为一种生物肥料在高污染土壤中具有提高重金属耐受性和促进芥菜生长的潜力,同时提高土壤整体PGP活性。
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来源期刊
World journal of microbiology & biotechnology
World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.30
自引率
2.40%
发文量
257
审稿时长
2.5 months
期刊介绍: World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.
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