Veterinary dermatology最新文献

{"title":"Dissemination of T Lymphocytes and Potential Immune-Escape Mechanisms of Bovine Skin Squamous Cell Carcinoma Regarding MHC-1 and PD-L1 Expression.","authors":"Sabah M Sameen, Waseem Al-Jameel","doi":"10.1111/vde.70130","DOIUrl":"https://doi.org/10.1111/vde.70130","url":null,"abstract":"<p><strong>Background: </strong>Bovine skin squamous cell carcinoma (SCC) is a malignant neoplasm in cattle triggered by sunlight exposure. Immune escape drives tumour survival and progression. Previous findings in humans have emphasised the prognostic value of immune checkpoint marker (PDL-1), major histocompatibility complex (MHC-1) and cytotoxic CD8 T cells in the context of cancer immune escape.</p><p><strong>Objectives: </strong>We examined the distribution of CD8⁺ T cells and the possible immune-escape mechanisms of SCC in relation to PD-L1 and MHC-1 expression and correlated marker expression with tumour grade.</p><p><strong>Animals: </strong>Twenty samples of bovine skin SCC were collected between 2025 and 2026.</p><p><strong>Materials and methods: </strong>Histopathological evaluation, including grading, was performed on SCC samples. Immunohistochemical examination was performed for all three proteins in relation to histological grade, substantiated by the mRNA levels of MHC-1 and PD-L1.</p><p><strong>Results: </strong>We observed a significant relationship between PD-L1 expression and grade, increasing from 33% in well-differentiated SCC to 100% in moderately and poorly differentiated SCC. PD-L1 mRNA levels in well- and moderately differentiated SCC remained comparable, whereas poorly differentiated SCC exhibited a significant upregulation. MHC-1 showed a significant grade-dependent decline, decreasing from 83% in well differentiated to complete loss in poorly differentiated. This progressive protein loss was corroborated at the transcriptional level. High CD8 T-cell levels were observed in 83% of well-differentiated SCC, with complete absence in poorly differentiated SCC.</p><p><strong>Conclusions and clinical relevance: </strong>This study emphasises the importance of PD-L1, MHC-1 and CD8 T-cell biomarkers as prognostic indicators in SCC. High PD-L1, low MHC-1 and CD8 represent potential immune-escape mechanisms for bovine SCC.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Two Induction Protocols for Oclacitinib in Dogs.","authors":"Paulina J C Popp, Ralf S Mueller, Laura Widorn, Laura Udraite-Vovk, Ulrike Mueller, Tamara Weitzer","doi":"10.1111/vde.70127","DOIUrl":"https://doi.org/10.1111/vde.70127","url":null,"abstract":"<p><strong>Background: </strong>Oclacitinib is approved for the treatment of canine atopic dermatitis (AD) and pruritus. The conventional administration protocol includes twice-daily oral dosing for 14 days with a subsequent reduction to once-daily administration. This reduction sometimes leads to an increase in pruritus.</p><p><strong>Hypothesis/objectives: </strong>The aim of this study was to compare the changes in skin lesions and pruritus following a shortened induction versus the conventional protocol for oclacitinib.</p><p><strong>Animals: </strong>Forty-five client-owned dogs with AD were randomised into two groups.</p><p><strong>Material and methods: </strong>The conventional protocol group (CP) received oclacitinib tablets at 0.4-0.6 mg/kg twice-daily for 14 days, then once-daily for 7 days. In the shortened induction protocol group (SIP), oclacitinib was administered twice-daily for 6 days, then once-daily for 15 days. Pruritus was assessed daily by the owners using a pruritus Visual Analog Scale (PVAS). Lesions were scored with the Canine Atopic Dermatitis Lesion Index (CADLI) at Day (D) 0 and D21.</p><p><strong>Results: </strong>Data from 40 dogs were available. Mean CADLI scores decreased from 13.4 to 8 in the CP group (p = 0.12) and from 12.9 to 6.9 in the SIP group (p = 0.01), respectively. Mean pruritus scores decreased from 6.4 to 2.1 and from 5.7 to 1.9, respectively (p < 0.0001 each). No between-group differences were observed. An increase in pruritus following dose reduction was seen in two dogs (CP) and one dog (SIP).</p><p><strong>Conclusions and clinical relevance: </strong>The SIP was not significantly different to the CP with respect to improvement in lesion severity or pruritus.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149013016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful Treatment of Onchocerca lupi Auricular Infestation Causing Severe Otitis Externa in a Dog.","authors":"Franco Martini, Manuela Schnyder, David Schmid, Nina Fischer","doi":"10.1111/vde.70125","DOIUrl":"https://doi.org/10.1111/vde.70125","url":null,"abstract":"<p><p>We report the first successfully treated case of auricular Onchocerca lupi infection in a dog in Switzerland, causing severe unilateral otitis externa. Diagnosis was confirmed by molecular analysis after nematode extraction. Combined therapy led to complete remission within 6 weeks, highlighting O. lupi as a differential for chronic otitis with mass formation.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Field Safety of the Second-Generation, Highly Selective JAK1 Inhibitor Atinvicitinib for the Treatment of Canine Atopic Dermatitis: A Multicentre, Blinded, Randomised, Placebo-Controlled Clinical Trial in Client-Owned Dogs.","authors":"Faris Jirjis, Timothy J Kowalski, Robin C Lynn, Lindsey Overbey, Qi Cao, Matthew Stock, Inka Kuhlmann","doi":"10.1111/vde.70106","DOIUrl":"https://doi.org/10.1111/vde.70106","url":null,"abstract":"<p><strong>Background: </strong>Janus kinase (JAK) inhibitors alleviate pruritus and clinical signs of canine atopic dermatitis (cAD).</p><p><strong>Objective: </strong>To confirm the optimal dosing regimen of atinvicitinib, a second-generation highly selective inhibitor of JAK1 in dogs.</p><p><strong>Animals: </strong>Sixty-one client-owned dogs, ≥ 12 months and ≥ 2 kg, with cAD.</p><p><strong>Materials and methods: </strong>In a randomised, masked, placebo-controlled clinical trial, dogs received atinvicitinib, with food, at 0.8-1.2 mg/kg, once daily for 28 days (q.d. group, n = 21) or 0.4-0.6 mg/kg twice daily for 14 days followed by once daily dosing for 14 days (b.i.d.-q.d. group, n = 21); or placebo b.i.d.-q.d. (control group, n = 19). Owners assessed pruritus (Day [D]0-7, D14, D28) using a Visual Analog Scale (PVAS); veterinary surgeons assessed skin lesions (D0, D28) using the cAD Extent and Severity Index, 4th iteration (CADESI-04). On D0, D14 and D28, blood and urine samples were collected. Treatment success was defined as a ≥ 50% reduction from baseline PVAS or CADESI-04 on D28. Proportions of dogs with ≥ 2 PVAS reduction, and reduction in PVAS and/or CADESI-04 from baseline, also were assessed.</p><p><strong>Results: </strong>Treatment success was 87.5% for the q.d. group (p = 0.011) and 73.3% for the b.i.d.-q.d. group (p = 0.033) (23.1% control group). There was ≥ 2 decrease in PVAS in 80.0% of q.d. dogs. (16.7% controls, p = 0.042). Compared to controls, atinvicitinib-treated dogs had a significantly greater reduction in PVAS (q.d., p = 0.003, b.i.d.-q.d., p = 0.001) and CADESI-04 (q.d. group, p = 0.046). Mean results of clinical pathological values remained within reference ranges. Treatment was well-tolerated.</p><p><strong>Conclusions and clinical relevance: </strong>Once-daily atinvicitinib was safe, well-tolerated and effective at rapidly reducing pruritus and resolving cAD-associated skin lesions.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148991416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to Letter to Editor by Hakala et al.","authors":"Rodrigo Plazola-Ortiz, Roger Collins, Sophie Forster, Darren Berger, Stephen King","doi":"10.1111/vde.70129","DOIUrl":"https://doi.org/10.1111/vde.70129","url":null,"abstract":"","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148991445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor: 'Fibroepithelial Polyps of the Ear Canal in Dogs: A Retrospective Analysis of 17 Cases'.","authors":"Carolina Mendes, Michela De Lucia","doi":"10.1111/vde.70124","DOIUrl":"https://doi.org/10.1111/vde.70124","url":null,"abstract":"","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148971443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiation-Induced Alopecia in Dogs With Nasal Tumours: Coat Type Influences Development and Onset.","authors":"Yuki Nemoto, Kirara Aoki, Aoi Yoshida, Hirona Ueno, Munekazu Nakaichi","doi":"10.1111/vde.70126","DOIUrl":"https://doi.org/10.1111/vde.70126","url":null,"abstract":"<p><strong>Background: </strong>Radiation-induced alopecia (RIA) is a common adverse effect of radiation therapy, yet factors influencing its development in dogs remain unclear.</p><p><strong>Objectives: </strong>To characterise acute RIA in dogs with nasal tumours and to evaluate associations with coat type and radiation dose.</p><p><strong>Animals: </strong>Thirty-eight client-owned dogs were treated with radiation therapy for nasal tumours.</p><p><strong>Materials and methods: </strong>Medical records were reviewed retrospectively. Dogs were classified by coat type (straight versus curly or wire-haired). Time to onset of alopecia was assessed as the interval from the first irradiation to the first detection of alopecia, and cumulative skin dose was calculated up to alopecia onset. Dogs developing acute RIA during radiation therapy were compared with those without RIA. Dogs developing alopecia only after completion of radiation therapy were excluded.</p><p><strong>Results: </strong>Seventeen of 34 dogs developed acute RIA during radiation therapy. Coat type was significantly associated with RIA development (p = 0.003), with all dogs with curly or wire-haired coats affected. These dogs developed RIA earlier than those with straight-haired coats (median 14 versus 20 days; p = 0.047). Within affected dogs, RIA developed first in regions receiving higher skin doses and subsequently progressed to regions receiving lower skin doses.</p><p><strong>Conclusions and clinical relevance: </strong>Coat type influences the development of acute RIA in dogs, with curly or wire-haired breeds showing earlier onset and greater extent of alopecia. Alopecia progression reflects regional skin dose distribution. These findings may assist clinicians in predicting the risk of RIA and informing owners about the potential development and timing of radiation-induced alopecia.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148921067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic Inflammation in Dogs With Atopic Dermatitis Compared to Healthy Dogs and Dogs With Pemphigus Foliaceus: A Study Using a Limited Cytokine Panel.","authors":"Ina Herrmann, Lisa B Mamo, Petra Bizikova","doi":"10.1111/vde.70118","DOIUrl":"https://doi.org/10.1111/vde.70118","url":null,"abstract":"<p><strong>Background: </strong>Atopic dermatitis (AD) is a common chronic, pruritic and inflammatory skin disease in both humans and dogs. In people, AD is increasingly recognised as a systemic inflammatory disease in which immune dysregulation drives chronic inflammation extending beyond the skin, particularly in moderate-to-severe disease. Whether a comparable systemic inflammation exists in canine AD, however, remains poorly characterised.</p><p><strong>Hypothesis/objectives: </strong>We hypothesised that serum cytokine concentrations would differ between dogs with varying AD severity and healthy controls. This study aimed to identify systemic inflammatory markers correlating with AD severity, by comparing cytokine profiles in dogs with mild AD and moderate-to-severe AD against healthy dogs (negative control) and dogs with active pemphigus foliaceus (PF) lesions (positive control).</p><p><strong>Animals: </strong>Forty client-owned dogs were enrolled, with 10 dogs in each of four groups: healthy controls, mild AD, moderate-to-severe AD and PF.</p><p><strong>Materials and methods: </strong>Serum concentrations of tumour necrosis factor (TNF)-α, TNF-β/lymphotoxin-α, interleukin (IL)-1β, IL-6, IL-12 and IL-1 receptor antagonist (IL-1RA) were measured using standardised immunoassays to evaluate systemic inflammation across groups.</p><p><strong>Results: </strong>Marked interindividual variability was observed across all groups. No statistically significant differences in serum concentrations of any of the six cytokines were detected between the healthy, mild AD, moderate-to-severe AD and PF groups.</p><p><strong>Conclusions and clinical relevance: </strong>In a limited cytokine panel, no evidence of differential systemic inflammation was detected across groups, including dogs with moderate-to-severe AD compared to healthy controls. These findings may reflect true lack of systemic inflammation in dogs or may be attributable to the small sample size, high interindividual variability or the limited scope of the cytokine panel evaluated.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison Between Colorimetric and Subjective Evaluation of Intradermally Injected Histamine-Induced Erythema in Healthy Dogs.","authors":"Ana Petak, Elisa Samuel, Svetlina Aleksandrova, Evi I Sofou, Manolis K Chatzis, Manolis N Saridomichelakis","doi":"10.1111/vde.70123","DOIUrl":"https://doi.org/10.1111/vde.70123","url":null,"abstract":"<p><strong>Background: </strong>Currently used methods for the interpretation of intradermal allergen test results either ignore the erythema of the wheals (objective method) or evaluate it subjectively (subjective method, and global wheal score [GWS]).</p><p><strong>Objectives: </strong>To compare the efficacy of GWSs in differentiating between wheals induced by intradermal injection of a negative control and histamine at three different concentrations, using either the objective colorimetric measurement of erythema or the subjective evaluation of erythema as absent, weak, or strong.</p><p><strong>Animals: </strong>Seven clinically healthy dogs.</p><p><strong>Materials and methods: </strong>Duplicate intradermal injections of a negative control, histamine 0.0001%, histamine 0.001%, and histamine 0.01% were administered on the lateral thorax of the dogs. A colorimeter was used to measure erythema before and 15 min after the injection and, at the same time, three blinded investigators scored erythema subjectively.</p><p><strong>Results: </strong>The subtraction of the first colorimetric measurement of erythema obtained before intradermal injection from the first postinjection objective measurement (ΔEfirst) outperformed three other objective measurement methods and yielded GWSs capable of differentiation among the four solutions with efficiencies comparable to those of GWSs based on subjective erythema assessment. However, the stronger correlation between the later and wheal diameters may indicate bias in the subjective evaluation of erythema.</p><p><strong>Conclusions and clinical relevance: </strong>A single colorimetric measurement of erythema before and after intradermal injection of a negative control and histamine at three different concentrations, followed by calculation of their difference, can replace the subjective evaluation of erythema for the calculation of GWSs and avoid possible bias.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148867005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Performance of Immunoglobulin E Serology, Prick Test and Patch Test With Food Allergens in Dogs With Atopic Dermatitis and Adverse Food Reactions Using the Double-Blind Placebo-Controlled Food Challenge as Gold Standard.","authors":"Evi I Sofou, Svetlina Aleksandrova, Elisa Samuel, Manolis Chatzis, Manolis N Saridomichelakis","doi":"10.1111/vde.70119","DOIUrl":"https://doi.org/10.1111/vde.70119","url":null,"abstract":"<p><strong>Background: </strong>In dogs with adverse food reactions (AFRs), immunoglobulin (Ig)E serology, prick test and patch test with food allergens have shown variable diagnostic performance for the identification of the offending foods, when the latter were identified using open food challenges.</p><p><strong>Objectives: </strong>To evaluate the diagnostic performance of these tests in dogs with atopic dermatitis (AD) and AFRs, using the double-blind placebo-controlled food challenge (DBPCFC) as the gold standard.</p><p><strong>Animals: </strong>Twelve dogs with AD and AFRs underwent DBPCFCs with eight food items.</p><p><strong>Materials and methods: </strong>IgE serology, prick test and patch test with the same eight food allergens were performed after the diagnosis of AD and before the diagnostic investigation for AFRs and the DBPCFCs.</p><p><strong>Results: </strong>The sensitivity (3%-60.6%), specificity (54.8%-98.4%), positive predictive value (25%-66.7%) and negative predictive value (64.1%-72.3%) of the three tests and of their combination were unsatisfactory. Furthermore, their positive and negative likelihood ratio values were not in the clinically informative range, apart from the prick test having a weakly informative positive likelihood ratio value of 3.81.</p><p><strong>Conclusions and clinical relevance: </strong>Under the conditions of this study, the diagnostic performance of IgE serology, prick test and/or patch test with food allergens was poor using the DBPCFC as gold standard.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148850822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}