Veterinary dermatology最新文献

{"title":"Using colorimetric in situ hybridisation method for FcaPV-2 to estimate postsurgical prognosis in feline Bowenoid in situ carcinoma.","authors":"Francesca Abramo, Maurizio Mazzei, Mario Forzan, Giorgia Giannetti, Francesco Albanese, Erica Melchiotti, Giordana Zanna, Marta Vascellari","doi":"10.1111/vde.13297","DOIUrl":"10.1111/vde.13297","url":null,"abstract":"<p><strong>Background: </strong>Feline Bowenoid in situ carcinoma (BISC) is frequently associated with Felis catus papillomavirus-2 (FcaPV-2). Although surgical excision of BISC is expected to be curative, recurrent lesions are reported and it is not known whether it is a consequence of incomplete surgery or residual viral load.</p><p><strong>Objectives: </strong>To combine colorimetric in situ hybridisation (CISH) and quantitative (q)PCR for the detection of viral DNA, and to correlate the clinical outcome of cats with BISC in which FcaPV-2 DNA is detected at surgical margins.</p><p><strong>Animals: </strong>Twenty-seven cats with a histopathological diagnosis of BISC.</p><p><strong>Materials and methods: </strong>Sections including core and margins of the lesions were used for histopathological evaluation, qPCR and CISH. After surgical removal of the lesion, clinical follow-up data were recorded for 6 months.</p><p><strong>Results: </strong>Six of 12 cases in which all four histological margins were evaluable were used to correlate the infection status at the margins with the follow-up data. Four showed margin positivity, of which half relapsed as expected and half cured; two cases were negative, of which one cured as expected while the other relapsed. Fifteen cases where only three, two or one of the histological margins were evaluable, were considered to adequately correlate the status of infection with the follow-up data if CISH was positive. Follow-up data were available for three with positive margins: one relapsed while the other two were cured.</p><p><strong>Conclusions and clinical relevance: </strong>Wide clinical surgical margins are always recommended for neoplastic conditions, yet there was no evidence that CISH margin examination would be beneficial in predicting recurrence in this viral-induced lesion.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"83-91"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of commercial next-generation sequencing assays to conventional culture methods for bacterial identification and antimicrobial susceptibility of samples obtained from clinical cases of canine superficial bacterial folliculitis.","authors":"Kimberly Smart, Jason B Pieper, Austin K Viall, James O Noxon, Darren J Berger","doi":"10.1111/vde.13299","DOIUrl":"10.1111/vde.13299","url":null,"abstract":"<p><strong>Background: </strong>Bacterial identification and antimicrobial susceptibility testing is an important step in timely therapeutic decisions for canine superficial bacterial folliculitis (SBF), commonly caused by Staphylococcus pseudintermedius. Next-generation sequencing (NGS) offers the appeal of potentially expedited results with complete detection of bacterial organisms and associated resistance genes compared to culture. Limited studies exist comparing the two methodologies for clinical samples.</p><p><strong>Hypothesis/objectives: </strong>To compare and contrast genotypic and phenotypic methods for bacterial identification and antimicrobial susceptibility from cases of canine SBF.</p><p><strong>Animals: </strong>Twenty-four client-owned dogs with lesions consistent with SBF were enrolled.</p><p><strong>Materials and methods: </strong>A sterile culturette swab was used to sample dogs with SBF lesions. The swab was rinsed in 0.9 mL of sterile phosphate-buffered saline and vortexed to create a homogenous solution. Two swabs for NGS laboratories (Labs) and one swab for culture (Culture Lab) were randomly sampled from this solution and submitted for bacterial identification and antimicrobial susceptibility.</p><p><strong>Results: </strong>No statistical difference regarding turnaround time for NGS Labs compared to Culture Lab was found. NGS Lab 1 identified more organisms than NGS Lab 2 and Culture Lab, which were both statistically significant. There was no statistical difference in detection frequency for Staphylococcus spp. among all laboratories. There was poor agreement for the presence of meticillin resistance and most antimicrobials among all laboratories.</p><p><strong>Conclusions and clinical relevance: </strong>Utilisation of NGS as a replacement for traditional culture when sampling canine SBF lesions is not supported at this time.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"14-23"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11696477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total protein concentration and stability of Amb a 1 in glycerinated ragweed sublingual immunotherapy stored at room temperature and refrigerated cold temperature.","authors":"Tyler Charbonneau, Levi Lowder, Glenna Mauldin, Brennan McKinney, Rebecca Mount, Anthea Schick","doi":"10.1111/vde.13310","DOIUrl":"10.1111/vde.13310","url":null,"abstract":"<p><strong>Background: </strong>Few studies have investigated optimal storage conditions or expiration dates for sublingual immunotherapy (SLIT) formulations prepared from glycerinated allergen extracts.</p><p><strong>Hypothesis/objectives: </strong>The objective of this study was to compare concentrations of short ragweed major allergen (Amb a 1) and total protein in SLIT formulations stored at two different temperatures. It was hypothesised that protein concentrations would show greater decline over time in a formulation stored at room temperature (RT) than in one stored under refrigeration.</p><p><strong>Materials and methods: </strong>Two SLIT samples containing equal volumes of 20,000 PNU Amb a 1 extract were prepared and stored at refrigerated cold (CT) (2-8°C) or RT (20-24°C) for 140 days. Changes in total protein and major allergen concentration and composition were measured by Bradford assay, two-site enzyme-linked immunosorbent assay and SDS-PAGE. Presence of Amb a 1 was confirmed with Western immunoblot. Data were analysed using an analysis of covariance, with p < 0.05 considered significant.</p><p><strong>Results: </strong>SDS-PAGE showed compositional changes in a ~26-30 kDa protein band under RT and not CT storage. The Amb a 1 concentration of the RT SLIT sample declined significantly over time, compared to that of the CT SLIT sample (F_(1,8) = 47.69, p < 0.0001). There was no significant difference in total protein concentration over time between groups (F_(1,8) = 1.79, p = 0.22).</p><p><strong>Conclusions and clinical relevance: </strong>These results demonstrate that storage of glycerinated SLIT formulations in refrigerated CT preserved the highest concentration of the specific allergen Amb a 1, suggesting that SLIT formulations containing short ragweed should be stored under refrigeration.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"4-13"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hair fragility (trichorrhexis nodosa) in alopecic Pomeranian dogs.","authors":"Erin Brennan, Jonna Juhola, Ewan A Ferguson, Anette Loeffler, Rosario Cerundolo, Anke Hendricks, Ross Bond","doi":"10.1111/vde.13296","DOIUrl":"10.1111/vde.13296","url":null,"abstract":"<p><strong>Background: </strong>While alopecia associated with hair cycle arrest (HCA, Alopecia X) is well-recognised in Pomeranian dogs, the authors are unaware of reports of concurrent hair fragility.</p><p><strong>Hypothesis/objectives: </strong>Following the observation of frequent hair shaft abnormalities in alopecic Pomeranians, we hypothesised that hair fragility events would be more frequent in dogs with a phenotype of HCA when compared to dogs with normal coats.</p><p><strong>Animals: </strong>Eight alopecic Pomeranian dogs or crosses with a phenotype of HCA and 36 unaffected Pomeranians with owner-reported normal hair coats.</p><p><strong>Materials and methods: </strong>All affected dogs underwent dermatological examination and clinicopathological evaluations. Hair samples, plucked from affected areas or obtained by brushing from unaffected dogs, were examined microscopically for structural abnormalities. Hair fragility events were characterised as trichorrhexis nodosa (TN) or longitudinal splits and were expressed per 10 mg of hair. A reference interval was calculated from the number of hair fragility events in the samples from unaffected dogs.</p><p><strong>Results: </strong>The upper reference limit (with 90% confidence) from samples of 35 unaffected Pomeranians (one outlier excluded) was 9.75 hair fragility events per 10 mg of hair. The median (range) of fragility events in eight dogs with a phenotype of HCA was 66.0 (30.2-166.7) per 10 mg of hair.</p><p><strong>Conclusions and clinical relevance: </strong>Clinicians should routinely perform trichography in Pomeranians with HCA because abundant hair abnormalities, particularly TN, may contribute to poor hair coat quality. Further studies are required to establish the pathophysiology of and treatments for these fragility events and to determine their predictive value for HCA.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"64-73"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11696478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of immunoglobulin E sensitisation to mites and pollens in 25,451 French dogs from 2016 to 2022.","authors":"Laurent Drouet, Silvia Bonati, Alicia Cozar, Nathalie Petit, Pascal Prélaud","doi":"10.1111/vde.13309","DOIUrl":"10.1111/vde.13309","url":null,"abstract":"<p><strong>Background: </strong>Canine atopic dermatitis (cAD) is a common skin disorder in dogs, wherein allergens are presumed to exert a significant influence. The prevalence of allergen sensitisation in dogs with suspected cAD remains inadequately investigated in France.</p><p><strong>Hypothesis/objectives: </strong>This study aimed to analyse allergen-specific immunoglobulin (Ig)E test results from French dogs with suspected cAD, exploring potential risk factors influencing sensitisation.</p><p><strong>Animals: </strong>The study included 25,451 client-owned dogs presented with clinical signs of cAD between January 2016 and December 2022. The study population consisted of 226 pure breeds and 915 cross-bred dogs of both sexes, with a mean age of 4.4 (±2.8) years.</p><p><strong>Materials and methods: </strong>Data collection involved the compilation of results from canine serological allergen-specific IgE ELISA tests, using a 23 allergen composite panel. The influence of breed, sex, age, geographical area and sampling season on IgE test outcomes subsequently was analysed.</p><p><strong>Results: </strong>Of the 25,451 analysed samples, 75.6% exhibited significant serum IgE concentrations in response to at least one of the tested allergens, with 67.9% testing positive for mites and 39.8% for pollens. Breed, sex, age, geographical area and sampling season were identified as significant risk factors influencing sensitisation patterns.</p><p><strong>Conclusions and clinical relevance: </strong>This study provides insights into the prevalence and risk factors associated with allergen sensitisation in dogs with suspected cAD. Understanding these factors is crucial for effective diagnosis and tailored management strategies in veterinary practice. The findings underscore the need for personalised approaches in addressing cAD, considering individual patient characteristics.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"52-63"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling of dorsal root ganglia in atopic and healthy dogs: A comparative RNA sequencing study with implications in cutaneous itch research.","authors":"Chie Tamamoto-Mochizuki, Santosh K Mishra","doi":"10.1111/vde.13324","DOIUrl":"https://doi.org/10.1111/vde.13324","url":null,"abstract":"<p><strong>Background: </strong>Itch is a common clinical sign in skin disorders. While the neural pathways of itch transmission from the skin to the brain are well understood in rodents, the same pathways in dogs remain unclear. The knowledge gap hinders the development of effective treatments for canine itch-related disorders.</p><p><strong>Hypothesis/objectives: </strong>This study aimed to investigate the differential gene expression in the dorsal root ganglia (DRGs) between healthy and atopic dogs to identify specific molecules potentially involved in itch signalling and neuroinflammation in canine atopic dermatitis (cAD).</p><p><strong>Animals: </strong>Two atopic and four healthy dogs.</p><p><strong>Materials and methods: </strong>DRGs were collected from atopic and healthy dogs to compare their transcriptional profiles using RNA sequencing.</p><p><strong>Results: </strong>Principal component and heatmap analyses revealed two distinct clusters separating atopic from healthy dogs. Consistent with this observation, we identified 627 (543 upregulated and 84 downregulated) differentially expressed genes (DEGs) in atopic compared with healthy dogs. We further narrowed down our genes of interest to common DEGs in each atopic dog, which revealed 159 (132 upregulated and 27 downregulated) DEGs. Among these genes, when we focused on itch signalling-associated molecules, P2RY12, IL-2RG, TLR1 and POSTN were significantly upregulated, while MRGPRD and LPAR3 were both significantly downregulated in atopic dogs compared with those in healthy dogs. Pathway analysis showed a significant upregulation of CREB signalling in neurons, myelination signalling and neuroinflammation signalling pathways in atopic dogs.</p><p><strong>Conclusions and clinical relevance: </strong>Our study suggested that dysregulation of neuroinflammatory pathways might play a role in the pathomechanism of cAD as in humans.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of Lactobacillus casei on Staphylococcus pseudintermedius isolates.","authors":"Danielle Cavalcanti de Carvalho Diniz, Marcio Garcia Ribeiro, Gabriele Silva Dias, Guilherme de Brito Viana, Adriano Sakari Okamoto, Luiz Henrique de Araújo Machado","doi":"10.1111/vde.13325","DOIUrl":"https://doi.org/10.1111/vde.13325","url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial resistance is increasing each year. For example, in 2019 it was directly responsible for an estimated >1 million deaths. Additionally, the development of new drugs is much slower, generating enormous concerns about responses to infection in the future health scenario. Therefore, probiotics have emerged as an alternative to antibiotics.</p><p><strong>Objectives: </strong>This study aimed to isolate and identify a Lactobacillus casei from healthy canine skin and investigate its antimicrobial effect on isolates of Staphylococcus pseudintermedius originating from dogs with pyoderma.</p><p><strong>Materials and methods: </strong>L. casei was isolated from skin samples collected with a sterile cotton swab from the inner pinnae of healthy dogs. It was then cultured, identified using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry, and tested against 30 different clinical isolates and one American Type Culture Collection strain of S. pseudintermedius using the spot-on-the-lawn technique. Its safety was assessed through a modified Kirby-Bauer disc diffusion susceptibility test.</p><p><strong>Results: </strong>L. casei inhibited the growth of all isolates of S. pseudintermedius. The mean value of the inhibition halo of all isolates was 11.3 mm. A significant positive correlation (Pearson's linear correlation = 0.444; p = 0.014) was noted between the inhibitory halos formed by L. casei on the S. pseudintermedius isolates and the halos produced by the tested antimicrobial discs on the same isolates. The L. casei strain demonstrated sensitivity to all tested antimicrobials.</p><p><strong>Conclusions and clinical relevance: </strong>The study indicates that using commensal bacteria from canine skin, specifically L. casei, to control bacterial infections caused by S. pseudintermedius can be a promising complementary or alternative therapy to antibiotics relevant to animal and human health.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical aspects of middle ear tympanokeratoma in dogs diagnosed through advanced imaging, otoendoscopy and histopathological evaluation.","authors":"Cristiane Bazaga Botelho, Rafaella Tortoriello, Sandra Nogueira Koch, Julio Israel Fernandes","doi":"10.1111/vde.13321","DOIUrl":"https://doi.org/10.1111/vde.13321","url":null,"abstract":"<p><strong>Background: </strong>Canine aural cholesteatoma (more appropriately named tympanokeratoma) is an epidermoid cyst whose aetiopathogenesis remains poorly recognised in veterinary medicine. There are a few reports published, possibly because it may be underdiagnosed.</p><p><strong>Objectives: </strong>To characterise the clinical aspects of dogs with tympanokeratoma, to describe the otoendoscopic, advanced imaging and histopathological findings of tympanokeratoma and to report the best approach to diagnose canine auricular tympanokeratoma in a retrospective study.</p><p><strong>Material and methods: </strong>Of 890 dogs with suspected tympanokeratoma and otitis media, 100 animals underwent advanced imaging and otoendoscopy at radiology and dermatology reference centres in Brazil.</p><p><strong>Results: </strong>Most affected dogs were male (71%) neutered (95%) with an average age of 6.8 years. Ninety-one of the 100 affected dogs were brachycephalic. Otitis externa (OE; 81%) was the main non-neurological manifestation observed. The main neurological clinical manifestations observed were: \"head tilt\" (66%), ataxia (31%) and nystagmus (25%). Advanced imaging findings could not propose a presumptive diagnosis of tympanokeratoma in 60 of 100 (60%) of the dogs. The absence of tympanic membrane and the presence of a dense pearly yellowish material resembling keratin in the tympanic bulla, after myringotomy, was the main otoendoscopic finding. The advanced imaging findings did not correlate with otoendoscopy and histopathological findings in more than half of the dogs.</p><p><strong>Conclusions and clinical relevance: </strong>Tympanokeratoma should be suspected in brachycephalic dogs with OE and peripheral vestibular syndrome, and samples of keratinous material from the middle ear associated with histopathological results may be the best approach for the diagnosis.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunotherapy using pullulan-conjugated Der f 2 allergen in canine atopic dermatitis: An anti-inflammatory agent-sparing approach.","authors":"Masahiko Nagata, Jin Kozakai, Aya Yoshida, Kana Kameyama, Hiromi Oboso, Hisanori Muto, Shoji Ogino, Toshihiro Tsukui","doi":"10.1111/vde.13315","DOIUrl":"https://doi.org/10.1111/vde.13315","url":null,"abstract":"<p><strong>Background: </strong>Allergen immunotherapy is used as aetiological treatment for canine atopic dermatitis (cAD).</p><p><strong>Objective: </strong>To assess the anti-inflammatory agent-sparing effect over 1 year of immunotherapy using pullulan-conjugated recombinant Der f 2 (rDf2-P).</p><p><strong>Animals: </strong>Twenty-one privately owned dogs with cAD.</p><p><strong>Materials and methods: </strong>Dogs with mild clinical signs after ≥4 weeks of anti-inflammatory drug treatment received rDf2-P immunotherapy for 1 year. A monthly medication score (MS) was calculated, and clinical signs were assessed using Canine Atopic Dermatitis Extent and Severity Index (CADESI)-04, cAD Eczema Area and Severity for Shiba Inu and pruritus scores. Serum thymus and activation-regulated chemokine (TARC)/C-C Motif Chemokine Ligand-17 (CCL17) concentrations were assessed at the initial and final doses during the 6-week induction phase and every 3 months for 1 year thereafter in 16 cases.</p><p><strong>Results: </strong>The mean MS decreased significantly by 43.0% (p = 0.022), 60.9% (p = 0.003), 70.0% (p = 0.0004), 58.7% (p = 0.0004) and 49.3% (p = 0.029) at 2.5, 4.5, 7.5, 10.5 and 13.5 months, respectively. Drug-sparing effects, assessed with MS and adjusted by clinical scores were rated as excellent for 12 (57.1%) and 11 (52.4%) dogs, good for 3 (14.3%) and 1 (4.8%) dogs, fair for 0 and 3 (14.3%) dogs, and poor for 6 (28.6%) and 5 (23.8%) dogs at 4.5 and 13.5 months, respectively. The serum TARC/CCL17 concentrations were significantly lower in the samples with lower CADESI-04 scores (<17) than in those with higher CADESI-04 scores (>17) (p = 0.0002).</p><p><strong>Conclusions and clinical relevance: </strong>Der f 2-P immunotherapy can lead to a rapid reduction in anti-inflammatory drug use and serve as an effective proactive therapy for cAD.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin barrier, phenotypic and genotypic characterisation of autosomal recessive ichthyosis in TGM1-deficient Jack Russell Terriers and response to topical ceramide.","authors":"Elizabeth Mauldin, Charles Bradley, Margret Casal, Jason Meyer, Debra Crumrine, Sarah Kiener, Tosso Leeb, Peter M Elias","doi":"10.1111/vde.13285","DOIUrl":"10.1111/vde.13285","url":null,"abstract":"<p><strong>Background: </strong>Autosomal recessive ichthyosis leads to structural or biochemical changes that impair skin barrier function.</p><p><strong>Hypothesis/objectives: </strong>To assess (1) the phenotype and genotype in a litter of Jack Russell Terriers with autosomal recessive congenital ichthyosis (ARCI), and (2) the defective skin barrier and determine if a topical ceramide can modulate the barrier.</p><p><strong>Animals: </strong>A healthy dam and litter of Jack Russell Terrier puppies (healthy male, affected male and female), one affected adult Jack Russell Terrier and one unrelated healthy Jack Russell Terrier.</p><p><strong>Materials and methods: </strong>A severe cornification defect was identified via examination of affected puppies. As the phenotype worsened, the affected puppies received a topical application of ω-0-acylceramide for 10 days. Before humane euthanasia, the skin barrier was evaluated via transepidermal water loss (TEWL), corneometry and pH in affected dogs. Genomic testing was performed, and skin samples were analysed by light and electron microscopy.</p><p><strong>Results: </strong>Affected puppies were homozygous for the 1980 bp LINE-1 insertion in the TGM1 (transglutaminase 1) gene; the unaffected littermate and the dam were heterozygous carriers. ARCI puppies were underweight and had a severe hyperkeratotic phenotype that impaired mobility. TEWL was markedly higher in affected dogs. The cutaneous pH of affected puppies was higher than the normal littermate. Treatment of the skin with ω-0-acylceramide normalised the pH to match the littermate and decreased TEWL. Electron microscopy revealed marked attenuation of the cornified envelope.</p><p><strong>Conclusions and clinical relevance: </strong>Dogs with TGM1-deficient ARCI have an impaired skin barrier. Topical therapy can partially repair the barrier defect.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"617-625"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}