薰衣草精油、耳用凝胶和庆大霉素对消除铜绿假单胞菌产生的生物膜的体外疗效。

IF 1.9 3区 农林科学 Q3 DERMATOLOGY
Veterinary dermatology Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI:10.1111/vde.13294
Ana Mourão, Isa Serrano, Eva Cunha, Luís Tavares, Ana Lourenço, Manuela Oliveira
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引用次数: 0

摘要

背景:外耳道炎(OE)是犬最常诊断出的皮肤病之一,其病因是多因素的。在与犬外耳道炎相关的细菌病原体中,铜绿假单胞菌尤其令人担忧,因为它经常具有多重耐药性,并能形成与感染的慢性化和复发有关的生物膜:本研究的主要目的是评估和比较两种创新抗菌剂--含有合成抗菌肽的耳科凝胶和薰衣草精油--与庆大霉素(一种传统抗生素)的抗生物膜活性:测定了庆大霉素、耳科凝胶和薰衣草精油对从 OE 犬耳道中获得的 35 个临床分离物中的 12 个铜绿假单胞菌生物膜产生者的生物膜根除能力。此外,还在体外狗耵聍模型中评估了耳科凝胶对铜绿假单胞菌生物膜的抗菌活性:结果:薰衣草油在接触 30 分钟后显示出最佳效果,可消灭 58.3% 的分离株(12 株中的 7 株),庆大霉素在 24 小时后显示出完全消灭的效果(12 株中的 12 株)。研究还发现,合成耵聍耳屎中的甘油三酯具有抗伪菌活性,与耳科凝胶结合使用时,可完全清除铜绿假单胞菌:这项体外研究的结果表明,薰衣草精油和耳科凝胶可作为庆大霉素的替代品,有效治疗由铜绿假单胞菌生物膜产生者引起的犬OE。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro efficacy of lavender oil, otological gel and gentamicin to eradicate biofilm produced by Pseudomonas aeruginosa.

Background: Otitis externa (OE) is one of the most frequently diagnosed dermatological diseases in dogs, having a multifactorial aetiology. Among the bacterial agents associated with canine OE, Pseudomonas aeruginosa is of special concern owing to its frequent multidrug resistance profile and ability to form biofilms related to the infection's chronicity and recurrence.

Objectives: The main objective of this study was to evaluate and compare the antibiofilm activity of two innovative antimicrobials-an otological gel containing a synthetic antimicrobial peptide and Lavandula angustifolia essential oil-with gentamicin (a conventional antibiotic) using biofilm-producing P. aeruginosa isolates obtained from dogs with OE.

Materials and methods: The biofilm eradication capacity of gentamicin, otological gel and lavender oil was determined against a collection of 12 P. aeruginosa biofilm-producers among 35 clinical isolates obtained from the ear canals of dogs with OE. Also, the antimicrobial activity of the otological gel against P. aeruginosa biofilms was assessed in an in vitro model of dog cerumen.

Results: Lavender oil showed the best effectiveness after 30 min of contact, eradicating 58.3% (seven of 12) of the isolates, and gentamicin showed full eradication (12 of 12) after 24 h. The otological gel acted more slowly than the lavender oil; yet at 24 h, the antibiofilm capacity of both compounds was similar, with no significant difference between them. It also was found that triglycerides from synthetic cerumen earwax had antipseudomonal activity and, when combined with the otological gel, led to the full eradication of P. aeruginosa.

Conclusions and clinical relevance: The results of this in vitro study indicate that lavender oil and the otological gel may be effective topical treatments for canine OE promoted by P. aeruginosa biofilm-producers, as alternatives to gentamicin.

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来源期刊
Veterinary dermatology
Veterinary dermatology 农林科学-兽医学
CiteScore
3.20
自引率
21.40%
发文量
92
审稿时长
12-24 weeks
期刊介绍: Veterinary Dermatology is a bi-monthly, peer-reviewed, international journal which publishes papers on all aspects of the skin of mammals, birds, reptiles, amphibians and fish. Scientific research papers, clinical case reports and reviews covering the following aspects of dermatology will be considered for publication: -Skin structure (anatomy, histology, ultrastructure) -Skin function (physiology, biochemistry, pharmacology, immunology, genetics) -Skin microbiology and parasitology -Dermatopathology -Pathogenesis, diagnosis and treatment of skin diseases -New disease entities
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