Chemical stability and in vitro antimicrobial efficacy of diluted silver sulfadiazine powder and cream over a six-month period.

IF 1.9 3区 农林科学 Q3 DERMATOLOGY
Veterinary dermatology Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI:10.1111/vde.13289
Christopher M Lopresto, Brian S Palmeiro, Stephen D Cole, Xin Xu, Jaclyn Dietrich, Darko Stefanovski
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引用次数: 0

Abstract

Background: Silver sulfadiazine (SSD) is commonly formulated into otic preparations to treat otitis externa, although evidence of stability and antimicrobial efficacy with long-term storage is lacking.

Objectives: To evaluate the effect of storage time on chemical stability and in vitro antimicrobial activity of SSD diluted in sterile water, including two 1% suspensions using SSD pharmaceutical-grade powder stored at room temperature (RT) in plastic or sterile glass bottles, and a 1:9 dilution using prescription SSD 1% cream stored at RT in a sterile glass bottle.

Materials and methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) assessed chemical stability. Trimethoprim/sulfamethoxazole-susceptible and trimethoprim/sulfamethoxazole-resistant strains of Staphylococcus pseudintermedius (SP), meticillin-resistant (MR) SP, S. schleiferi (SS), MRSS, Pseudomonas aeruginosa, Proteus mirabilis and Escherichia coli evaluated by 24 h time-kill analysis assessed in vitro antimicrobial efficacy. Each assessment was performed at zero, one, three and six months of storage.

Results: LC-MS/MS showed no significant change in concentration over time for any suspension. When adjusted for time and species/strain, all SSD suspensions showed significant reductions in colony forming units (cfu)/mL at 24 h (p < 0.001). Including all suspensions, a bactericidal effect (minimum 3-log cfu/mL reduction at 24 h) occurred against 94% of total isolates, with failure against 33 of 552 isolates (6%). Bactericidal failure was more likely with the cream-based suspension (p < 0.05) and at six months (p < 0.01).

Conclusions and clinical relevance: Powder-based and cream-based SSD/sterile water suspensions showed no significant change in concentration and demonstrated in vitro antimicrobial activity for six months. Bactericidal failure was more likely with the cream-based suspension and after six months of storage.

稀释的磺胺嘧啶银粉和乳膏在六个月内的化学稳定性和体外抗菌效果。
背景:磺胺嘧啶银(SSD)通常配制成治疗外耳道炎的耳用制剂,但缺乏长期储存稳定性和抗菌效果的证据:目的:评估储存时间对稀释在无菌水中的 SSD 的化学稳定性和体外抗菌活性的影响,包括两种 1%的悬浮液(使用在室温(RT)下储存在塑料瓶或无菌玻璃瓶中的 SSD 药用级粉末),以及一种 1:9 的稀释液(使用在室温(RT)下储存在无菌玻璃瓶中的处方 SSD 1%乳膏):液相色谱-串联质谱法(LC-MS/MS)评估化学稳定性。通过 24 小时时间杀灭分析,对假中间葡萄球菌(SP)、耐甲氧西林(MR)SP、施莱佛氏葡萄球菌(SS)、MRSS、铜绿假单胞菌、奇异变形杆菌和大肠埃希菌的三甲氧苄青霉素/磺胺甲噁唑敏感菌株和三甲氧苄青霉素/磺胺甲噁唑耐药菌株进行体外抗菌效力评估。每项评估分别在储存零、一、三和六个月时进行:结果:LC-MS/MS 显示,任何悬浮液的浓度均未随时间发生显著变化。经时间和物种/菌株调整后,所有 SSD 悬浮液在 24 小时后菌落形成单位(cfu)/毫升均显著减少(p 结论与临床相关性):粉状和膏状 SSD/无菌水悬浮液的浓度没有明显变化,体外抗菌活性持续了六个月。膏状悬浮液在储存六个月后更容易出现杀菌失败。
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来源期刊
Veterinary dermatology
Veterinary dermatology 农林科学-兽医学
CiteScore
3.20
自引率
21.40%
发文量
92
审稿时长
12-24 weeks
期刊介绍: Veterinary Dermatology is a bi-monthly, peer-reviewed, international journal which publishes papers on all aspects of the skin of mammals, birds, reptiles, amphibians and fish. Scientific research papers, clinical case reports and reviews covering the following aspects of dermatology will be considered for publication: -Skin structure (anatomy, histology, ultrastructure) -Skin function (physiology, biochemistry, pharmacology, immunology, genetics) -Skin microbiology and parasitology -Dermatopathology -Pathogenesis, diagnosis and treatment of skin diseases -New disease entities
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