Veterinary Medicine and Science最新文献

{"title":"Granulosa Cell Tumour in a Spayed Cat With Ovarian Remnant Syndrome and Hyperestrogenaemia Without Estrus Signs.","authors":"Hitomi Shinoda, Kenji Mori, Hiroshi Mori, Kumiko Ishigaki, Kazushi Asano","doi":"10.1002/vms3.71037","DOIUrl":"10.1002/vms3.71037","url":null,"abstract":"<p><strong>Background: </strong>Granulosa cell tumour (GCT) is the most common primary ovarian neoplasm in cats and may arise from residual ovarian tissue following incomplete spay procedures.</p><p><strong>Objectives: </strong>To describe the surgical management of a GCT identified in the setting of Ovarian Remnant Syndrome (ORS) in a spayed cat with hyperestrogenaemia without estrus signs, and to assess the potential utility of serum estradiol as an adjunct for diagnosis and postoperative monitoring.</p><p><strong>Methods: </strong>A 6-year-old female domestic shorthair cat, reportedly spayed, presented with a 2-week history of weight gain and abdominal distension. Diagnostic imaging and cytology suggested a large neuroendocrine tumour occupying the abdominal cavity. Exploratory laparotomy was performed, and the abdominal mass along with one hepatic nodule was resected.</p><p><strong>Results: </strong>Histopathology confirmed GCT in the abdominal mass with hepatic metastasis. A preoperative serum sample collected at initial presentation and assayed after receipt of the histopathology report showed markedly increased estradiol. Although clinical signs improved after surgery, the cat re-presented 4 weeks later with abdominal distension and severe anaemia; imaging revealed progression of hepatic lesions, and the patient died 5 weeks after surgery.</p><p><strong>Conclusions: </strong>This case highlights that GCT can occur in the setting of ORS without estrus signs, despite marked hyperestrogenaemia. Ovarian remnants may undergo neoplastic transformation and cause life-threatening disease, even in the absence of estrus signs. Serum estradiol may be a valuable marker for diagnosis, treatment response, and recurrence monitoring in feline GCT.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71037"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13281160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148279020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of a Pregnancy-Associated Glycoprotein-Based Lateral Flow Assay for Early Pregnancy Detection in Goats.","authors":"Mehmet Can Kara, Ayşe Merve Köse","doi":"10.1002/vms3.71022","DOIUrl":"10.1002/vms3.71022","url":null,"abstract":"<p><strong>Background: </strong>The significant cross-reactivity between bovine, ovine, and caprine pregnancy-associated glycoproteins (PAGs) enables the adaptation of bovine-specific diagnostics for use in other ruminants; consequently, the Alertys OnFarm Pregnancy Test (AOFPT)-a blood-based lateral flow assay-provides a rapid and practical solution for pregnancy detection directly at the animal's head under field conditions.</p><p><strong>Objectives: </strong>This study evaluated to assess and validate the performance of AOFPT in goats at days 21 and 28 post-mating, by comparing the results with serum progesterone (P4) analysis and using transabdominal ultrasonography (TAUS) as the gold standard.</p><p><strong>Methods: </strong>The study involved 85 Kilis goats, five months post-partum. Estrus was synchronized using an 11-day progestagen device, d-cloprostenol, and PMSG. Whole blood and serum samples were collected on Days 21 and 28 post-mating. AOFPT was performed on-farm immediately after collection. For validation, serum progesterone concentrations were measured via electrochemiluminescence immunoassay, and TAUS was performed on Days 35 and 42, with Day 42 findings serving as the gold standard.</p><p><strong>Results: </strong>Results indicated that on Day 21, AOFPT sensitivity, specificity, positive predictive value, and negative predictive value were 79.4%, 93.8%, 98.2%, and 51.7%, respectively. By Day 28, these metrics reached 100%, 81.3%, 95.8%, and 100%. Statistical agreement between AOFPT and the reference method was K = 0.55 (82.14%) on Day 21 and K = 0.87 (96.43%) on Day 28 (p <0.001). Median ± interquartile range (IQR) of P4 concentrations were 5.95±2.60 ng/mL on day 21 and 6.92 ± 2.93 ng/mL on Day 28. AOFPT-identified pregnant goats exhibited significantly higher P4 levels (p < 0.001) than non-pregnant goats on Day 21 (5.98 ± 3.21 vs. 4.05 ± 5.46) and Day 28 (6.84 ± 3.06 vs. 0.75 ± 5.96).</p><p><strong>Conclusions: </strong>AOFPT demonstrated accuracy and reliability closely matching P4 measurements and the reference method. This test provides a practical tool for early on-farm pregnancy diagnosis in goats, potentially enhancing reproductive management and productivity in dairy goat farms and large herds.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71022"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13248982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148206517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Status of On-Farm Artificial Insemination Services and Effectiveness of Oestrus Synchronization in Dairy Cattle Under Small-Scale Dairy Farming System.","authors":"Temesgen Milkias, Chencha Chebo, Alemayehu Worku","doi":"10.1002/vms3.71035","DOIUrl":"10.1002/vms3.71035","url":null,"abstract":"<p><strong>Background: </strong>Hormonal synchronization of oestrous has been an available technology to improve the efficiency of field artificial insemination (AI) in the dairy sector.</p><p><strong>Objective: </strong>This study was aimed at investigating the breeding practices, status of AI and effectiveness of oestrus synchronization (ES) on small-scale dairy farming in selected districts of the Wolaita Zone.</p><p><strong>Methods: </strong>Cross-sectional survey and retrospective data on the oestrus synchronization were collected and analysed. Descriptive statistics, chi-square tests, and indexed ranking procedures were applied to summarize the study data.</p><p><strong>Results: </strong>The predominant mating system practiced by dairy farmers was AI (55.6%), and 73.5% of farmers apply controlled mating. The highest response rate to PGF₂α administration was obtained for cattle of Sodo town (89.5%), followed by Boloso Sore (78.8%). A 30%-45.2% pregnancy rate was obtained across the study districts. Crossbred cattle have shown significantly (p > 0.001) higher response (87.1%) and pregnancy rates (47.9%) than the local animals. Likewise, 9.6%-18.5% of the birth rate was obtained from this study. Farmers' overall perceptions and desire to continue using oestrus synchronization were improving as a result of their application experiences over time. Moreover, shortage of AI inputs and AI technicians, heat detection skills, and inconsistency of the AI service during festivals, holidays, and weekends were reported as the major challenges prevailing in the study districts.</p><p><strong>Conclusions: </strong>The study provided insights on improving input supply, accurate heat detection and intensified cattle management are necessities to enhance field AI services and increase pregnancy rates in synchronized animals.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71035"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-Port Totally Laparoscopic Colonopexy for the Treatment of Recurrent Rectal Prolapse in a Cat: A Case Report.","authors":"Maximiliano Adrián Cifuentes Laponio","doi":"10.1002/vms3.71011","DOIUrl":"10.1002/vms3.71011","url":null,"abstract":"<p><strong>Objective: </strong>To describe a modified totally laparoscopic colonopexy technique performed using only two ports for the treatment of recurrent rectal prolapse in a cat, aimed at minimizing surgical access and postoperative morbidity.</p><p><strong>Study design: </strong>Case report.</p><p><strong>Animals or sample population: </strong>One adult feral domestic shorthair cat.</p><p><strong>Methods: </strong>A 3.4-kg adult male cat, with recurrent rectal prolapse following failure of conservative management, underwent a totally laparoscopic colonopexy using a two-port configuration. The procedure was performed through two 5-mm ports without colonic exteriorization. Serosal preparation of the descending colon and the left abdominal wall was achieved using monopolar electrocautery, followed by intracorporeal fixation with a continuous barbed polydioxanone suture. Surgical time, intraoperative findings, and postoperative outcome were recorded. Follow-up included clinical evaluation and abdominal ultrasonography.</p><p><strong>Results: </strong>The procedure was completed without intraoperative complications, with a total surgical time of 43 min. Postoperative handling and medical management were limited due to the patient's aggressive behaviour. The cat resumed voluntary feeding within 6 h postoperatively and showed no signs of tenesmus or recurrence of rectal prolapse during short-term follow-up. Ultrasonographic evaluation at 30 days confirmed stable fibrous adhesion between the descending colon and the abdominal wall, with no evidence of recurrence or complications.</p><p><strong>Conclusions: </strong>A two-port totally laparoscopic colonopexy provided effective stabilization of the colon and successful resolution of recurrent rectal prolapse in this cat. This approach may represent a valuable minimally invasive alternative in selected feline patients, particularly those with behavioural limitations that restrict postoperative handling and medical management.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71011"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13240579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148144965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determinants of Productivity and Sustainability in Small-Scale Dairy Industries: Evidence From the Salale Zone of Oromia, Ethiopia.","authors":"Girma Megersa Geleta","doi":"10.1002/vms3.71012","DOIUrl":"10.1002/vms3.71012","url":null,"abstract":"<p><p>The small-scale dairy industry plays a crucial role in improving rural livelihoods, nutrition and income generation in Ethiopia. However, its productivity and sustainability are constrained by socio-economic, institutional and climatic factors. This study identifies the key determinants influencing productivity and sustainability in the small-scale dairy sector of the Salale Zone, Oromia, Ethiopia. Primary data were collected from 250 smallholder dairy farmers using structured questionnaires. Ordered Logit regression model with marginal effects estimation was employed to assess factors affecting the likelihood of achieving higher dairy productivity under varying household, institutional and environmental conditions. The results indicate that age of household head (β = 0.012), education level (β = 0.040), land ownership (β = 0.490), access to social networks (β = 0.188), infrastructure (β = 0.150) and veterinary services (β = 0.160) significantly enhanced productivity. Conversely, larger household size (β = -0.038), low economic status (β = -0.275), market inefficiencies (β = -0.230), high feed prices (β = -0.290), milk adulteration (β = -0.270), price fluctuations (β = -0.340), seasonal feed shortages (β = -0.175) and climatic variability (β = -0.250) reduced performance. Marginal effects analysis showed that market inefficiencies, adulteration and price fluctuations decreased dairy success by 35%, 29% and 22%, respectively, while improved feed access, infrastructure, finance and training increased it by 31%, 27%, 24% and 18%. The findings highlight the combined influence of socio-economic, institutional and climatic factors on dairy productivity and provide empirical insights for enhancing efficiency, profitability and sustainability in Ethiopia's small-scale dairy industry.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71012"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13239679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148151501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Risk Factors of Q Fever in Smallholder Dairy Farms in Kenya.","authors":"Joseph Samuel Kimatu, Jackson Nyarongi Ombui, Timothy Muthui Wachira, Joseph Wasonga, Sylvia Cheptoo, Benson Rukwaro, Susan Migeni, Christine Mutisya, Getrude Nangekhe, Benedict Karani, Gideon Ndambuki, Erhan Yalcindag, Barend Mark de Clare Bronsvoort, Lina González Gordon, Elizabeth Anne Jessie Cook","doi":"10.1002/vms3.71036","DOIUrl":"10.1002/vms3.71036","url":null,"abstract":"<p><strong>Background: </strong>Q fever is a neglected zoonotic disease of global concern. In Kenya, it ranks among the top priority zoonoses, yet data on its prevalence and associated risk factors in smallholder dairy farms are limited.</p><p><strong>Objectives: </strong>To estimate the seroprevalence and molecular prevalence of Coxiella burnetii and identify risk factors for exposure in dairy cattle on smallholder farms in Kenya.</p><p><strong>Methods: </strong>A cross-sectional study was conducted on 448 smallholder dairy farms across Nandi County and nearby dairy-producing areas. Serum (n = 1829), vaginal swabs (n = 1783) and preputial swabs (n = 52) were collected. Animal and herd-level data on Q fever risk factors were recorded. An indirect IgG ELISA was used for antibody detection, whereas real-time polymerase chain reaction (PCR) (quantitative PCR [qPCR]) was performed on the swab samples.</p><p><strong>Results: </strong>The animal-level seroprevalence of C. burnetii was 8.7% (95% CI: 7.4-10.0), whereas herd-level seroprevalence was 29.0% (95% CI: 24.8-33.2). Molecular prevalence detected by qPCR was 1.5% (95% CI: 0.9-2.0). Cows with a history of abortion had increased odds of seropositivity (OR = 1.73, 95% CI: 1.14-2.61). Cattle on farms where dogs were kept-whether fed placentas or not-had lower odds of seropositivity compared to farms without dogs.</p><p><strong>Conclusion: </strong>This study confirms circulation of Q fever in smallholder dairy herds in Kenya. Investigating environmental persistence, farm management, interactions between susceptible hosts and sociocultural aspects of dog ownership in smallholder settings is essential to unravel the local transmission dynamics of Q fever. These findings highlight the need for coxiellosis control strategies, including improved biosecurity, surveillance and evaluation of vaccination approaches that have been successfully applied in other endemic settings.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71036"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13312989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148346280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastroscopic Evaluation of Gastric Mucosa in Horses Treated With Flunixin Meglumine or High-Dose Firocoxib: A Descriptive Pilot Study.","authors":"Renato Abrantes de Oliveira, Alexandre da Silva Correa, Francisco Décio de Oliveira Monteiro, Pedro Paulo Maia Teixeira","doi":"10.1002/vms3.71061","DOIUrl":"10.1002/vms3.71061","url":null,"abstract":"<p><strong>Background: </strong>Equine Gastric Ulcer Syndrome (EGUS) is common in horses, and nonsteroidal anti-inflammatory drugs (NSAIDs) are a known risk factor for gastrointestinal ulceration. Although COX-2 selective NSAIDs such as firocoxib theoretically offer a better safety profile, endoscopic evidence for their mucosal safety at high off-label doses is lacking.</p><p><strong>Objectives: </strong>To compare the effects of label-dose flunixin meglumine and high-dose firocoxib on the gastric mucosa of a small selected group of horses using serial gastroscopy and histopathology in a descriptive pilot study.</p><p><strong>Methods: </strong>Twenty mixed-breed horses were allocated non-randomly to control (n = 10), flunixin meglumine (1.1 mg/kg IM, n = 5), or firocoxib (1.0 mg/kg PO, n = 5) groups, treated once daily for 5 days. Gastroscopic evaluation and histopathological analysis were performed before (Day 1) and after treatment (Day 6).</p><p><strong>Results: </strong>No glandular disease was observed. Low prevalence of pre-existing squamous lesions was found in all groups without progression after treatment. Histopathological scores revealed baseline incomparability, with marked cellular infiltrate present only in the flunixin group at Day 1 (60%). Within-group analyses showed no significant changes between time points (p > 0.05). The use of NSAIDs effectively resolved lameness.</p><p><strong>Conclusions: </strong>In this small descriptive pilot study, limited by non-randomised allocation and small sample size, the study found no detectable short-term gastric mucosal injury associated with high-dose firocoxib within the limits of this study. Due to the non-randomised design, no causal conclusions can be drawn. These hypothesis-generating findings require confirmation in adequately powered randomised trials.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71061"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13344725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood Protozoan Infection Disrupts Ovarian Follicular Development in Dairy Cattle: A Mechanistic Link to Reproductive Failure.","authors":"Most Mousumi Akter, Md Sadequl Islam, Md Ashraful Islam, Md Mahmudul Hasan, Rakibul Islam, Md Mominul Islam, Md Gausur Rahman, Md Shafiqul Islam, Anup Kumar Talukder, Md Bazlar Rashid","doi":"10.1002/vms3.71115","DOIUrl":"10.1002/vms3.71115","url":null,"abstract":"<p><strong>Background: </strong>Haemoprotozoan infections are a pervasive threat to livestock health in tropical regions, yet their specific impacts on reproductive function remain unidentified.</p><p><strong>Objective: </strong>This study tested the hypothesis that blood protozoan infection induces anaemia and disrupts ovarian follicular dynamics, thereby contributing to infertility.</p><p><strong>Method: </strong>Forty cows (n = 40) with reproductive complaints were examined, and blood samples were analysed for protozoan infection and haemoglobin (Hb) concentration. Ten cyclic cows without any reproductive problems were served as controls. Ex vivo assessments were performed on reproductive tracts (n = 30) collected from an abattoir, followed by histopathological examination of ovarian tissues. A complementary rabbit (n = 10) model was conducted to assess haematological and iron metabolic changes across the oestrous cycle.</p><p><strong>Result: </strong>Among 30 symptomatic cows, 100% tested positive for blood protozoan infection, with distribution rates of 53.3% (8/15) in anoestrus, 40.0% (4/10) in repeat breeding and 20.0% (1/5) in conception failure. Infected cows exhibited significantly lower Hb levels (6.6 g%) compared to non-infected controls (10.6 g%). Cows with Hb ≤6.5 g% exhibited rudimentary ovaries, small to medium follicles and follicular cysts, whereas those with Hb ≥7 g% showed normal ovarian morphology. Histopathology confirmed cystic degeneration, fibrosis and aberrant follicular structures in infected cows. The rabbit model showed a significant decline in Hb and disruptions in serum iron levels during the anoestrus phase, reinforcing the link between iron metabolism and reproductive cyclicity.</p><p><strong>Conclusion: </strong>Our findings demonstrate that subclinical blood protozoan infection severely impairs ovarian health through anaemia-induced hypoxia and metabolic disturbances, establishing a direct link to infertility.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71115"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13387587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypertrophic Osteopathy Associated With Primary Pulmonary Histiocytic Sarcoma in a Dog.","authors":"Valentina Rinaldi, Francesca Ridolfi, Riccardo Finotello, Martina Rosto, Alessia Luciani, Andrea Boari, Paolo Emidio Crisi","doi":"10.1002/vms3.71021","DOIUrl":"10.1002/vms3.71021","url":null,"abstract":"<p><p>Hypertrophic osteopathy (HO) is a paraneoplastic or parainflammatory syndrome characterised by painful periosteal new bone formation and soft tissue swelling, most commonly associated with thoracic neoplasia. The pathogenesis of HO remains incompletely understood and may involve neurovascular mechanisms, altered pulmonary filtration of megakaryocytes and platelets and increased release of growth factors such as vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). This report describes the first documented case of HO associated with primary pulmonary histiocytic sarcoma (PHS) in a dog. A 7-year-old male Golden Retriever was referred for evaluation of diffuse pain, limb swelling and lameness. Diagnostic imaging revealed bilateral periosteal proliferation affecting the forelimbs and a pulmonary mass within the right caudal lung lobe. Histopathology and immunohistochemistry following lung lobectomy and lymphadenectomy confirmed a diagnosis of PHS with nodal metastasis. Surgical excision resulted in complete clinical resolution of pain despite persistent radiographic evidence of HO. Subsequent disease progression occurred despite multiple chemotherapy protocols, including lomustine, vinorelbine, and metronomic chemotherapy; however, the dog remained consistently pain-free throughout follow-up. Overall survival time was 450 days. This case expands the spectrum of neoplasms associated with HO and further supports the hypothesis that tumour location, rather than histotype, plays a central role in the development of this syndrome. Moreover, the sustained absence of pain despite persistent periosteal lesions and metastatic progression suggests that tumour burden and tumour microenvironment may significantly influence clinical manifestations of HO.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71021"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13261293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148227773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Antiparasitic Effects of Silver Nanoparticles on Sarcocystis Bradyzoites.","authors":"Nasrin Karimi, Mir-Hassan Moosavy, Nasser Hajipour, Hamed Behniafar","doi":"10.1002/vms3.71092","DOIUrl":"10.1002/vms3.71092","url":null,"abstract":"<p><strong>Background: </strong>Sarcocystis is a protozoan parasite of significant zoonotic and economic importance, primarily transmitted through the consumption of contaminated meat. Conventional antiparasitic drugs show limited efficacy and potential side effects, leading to growing interest in nanotechnology-based alternatives.</p><p><strong>Objective: </strong>This study evaluated the in vitro antiparasitic activity of silver nanoparticles (AgNPs) against Sarcocystis bradyzoites isolated from sheep.</p><p><strong>Methods: </strong>Macroscopic cysts were collected from infected sheep muscles at the Tabriz abattoir, and bradyzoites were purified and confirmed to be viable using trypan blue staining and a bioassay in cats. The parasites were exposed to AgNP concentrations of 100, 200, 400 and 800 µg/mL for 10, 30, 60 and 120 min, in triplicate. Untreated samples (phosphate-buffered saline [PBS]) and 5% NaCl (positive control) were included for comparison. Data were analysed using one-way ANOVA (p < 0.05).</p><p><strong>Results: </strong>The results demonstrated that a dose- and time-dependent increase in mortality, ranging from 20.11% ± 2.49% at 100 µg/mL after 10 min to 59.53% ± 8.52% at 800 µg/mL after 120 min. Although silver nanoparticles (AgNPs) did not achieve complete inactivation, their significant effect compared to controls (p < 0.05) indicates moderate in vitro antiparasitic activity.</p><p><strong>Conclusion: </strong>AgNPs exhibited measurable in vitro antiparasitic activity against Sarcocystis bradyzoites, suggesting their promise as an adjunct or alternative to traditional drugs. Further in vivo studies are recommended to assess safety, optimal dosing and practical applications in veterinary and food safety contexts.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71092"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13372013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}