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Effects of Intrauterine Isoproterenol Administration on Ovarian Follicular Development in Cows. 子宫内异丙肾上腺素对奶牛卵泡发育的影响。
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70198
Vefa Tohumcu, Mehmet Cengiz, A Hayirli, K Altinkaynak, Emre Arslanbas, Alper Yasin Ciplak, S Aydın, Omercan Alat
{"title":"Effects of Intrauterine Isoproterenol Administration on Ovarian Follicular Development in Cows.","authors":"Vefa Tohumcu, Mehmet Cengiz, A Hayirli, K Altinkaynak, Emre Arslanbas, Alper Yasin Ciplak, S Aydın, Omercan Alat","doi":"10.1002/vms3.70198","DOIUrl":"https://doi.org/10.1002/vms3.70198","url":null,"abstract":"<p><strong>Background: </strong>Isoproterenol (ISO) is a nonselective beta-adrenergic receptor agonist known for its vasodilatory effects. This experiment aims to investigate whether intrauterine ISO administration could alter vascular indices and follicular development in postpartum Holstein cows.</p><p><strong>Objectives: </strong>The objectives are to evaluate the effects of intrauterine ISO administration on vascular changes and its impact on follicular development compared to placebo groups.</p><p><strong>Study design: </strong>This randomized controlled study was conducted on 36 Holstein cows selected based on their health status, including only those free from reproductive, metabolic and infectious disorders.</p><p><strong>Methods: </strong>The cows (n = 36) were divided into two groups as control received distilled water alone (CON, n = 18) and experiment received 4 mg ISO in 40 mL distilled water (ISO, n = 18) and four subgroups as CON-I (n = 9), CON-II (n = 9), ISO-I (n = 9) and ISO-II (n = 9) according to days of intrauterine administration (I or II represents to 1 or 2 days after ovulation, respectively). Uterine and ovarian artery blood flows were assessed before and after administration by Doppler ultrasonography. Blood samples were collected both before and after administration (on Day 1 or 2) and on Days 3, 6 and 9 post-ovulation for hormonal analysis. Antral follicle count (AFC) was recorded on the blood sampling days. Data were analysed via mixed model ANOVA.</p><p><strong>Results: </strong>Intrauterine ISO administration significantly increased the pulse rate (PR) in the ovaries (89.4 vs. 65.5 bpm, p < 0.0001) and uterus (90.6 vs. 64.2 bpm, p < 0.0001). Early AFC (1-2.9 mm) decreased, whereas small AFC (3-4.9 mm) increased in the ISO groups. The weighted average antral follicle size (WAAFS) significantly increased in the ISO group but remained unchanged in the controls. Hormonal analysis revealed elevated levels of FSH (626 vs. 468 mIU/mL), AMH (61.3 vs. 46.4 ng/L), E2 (138 vs. 122 ng/L), P4 (15.3 vs. 10.6 ng/mL), IGF-1 (62.6 vs. 25.1 ng/mL) and IGFBP-3 (28.4 vs. 16.5 ng/mL) in the ISO groups (p < 0.0001).</p><p><strong>Conclusions: </strong>The findings indicate that intrauterine administration of ISO on Day 1 post-ovulation could be a promising 'adjunct technique' for future research focussed on minimizing dependence on exogenous hormones or improving the sensitivity of follicles to endogenous hormonal signals, thereby potentially enhancing oocyte yield.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70198"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection and Whole Genome Amplification of the 4d Type of Porcine Hepatitis E Virus in Eastern Tibet, China. 藏东地区猪戊型肝炎病毒4d型的检测及全基因组扩增
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70194
Yongzhi Lou, Jiaojiao Xin, Sizhu Suolang, Da Qiong, Zhuoma Dawa, Ga Gong
{"title":"Detection and Whole Genome Amplification of the 4d Type of Porcine Hepatitis E Virus in Eastern Tibet, China.","authors":"Yongzhi Lou, Jiaojiao Xin, Sizhu Suolang, Da Qiong, Zhuoma Dawa, Ga Gong","doi":"10.1002/vms3.70194","DOIUrl":"10.1002/vms3.70194","url":null,"abstract":"<p><p>Genomic and evolutionary analysis of epidemic porcine hepatitis E virus (HEV) in the Tibetan Plateau was performed. Faecal samples were collected from 216 Tibetan pigs and 78 Tibetan Yorkshire (Large White) and 53 tissue samples from Yorkshire from the Linzhi City slaughterhouse. Total RNA was extracted from faeces and fragments of HEV open reading frame 2 (ORF2) detected by reverse transcription and nested polymerase chain reaction (RT-nPCR) and cloned. Twenty-three samples (23/347; 6.63%) were positive for the virus, including 6.94% (15/216) Tibetan pig and 6.11% (8/131) Yorkshire samples. No tissue samples tested positive for the virus. Cloned sequences were uploaded to GenBank (accession numbers: OR392679-OR392685, OR355817-OR355824 and OR909495-OR909502) and a phylogenetic tree constructed. The entire viral genome was amplified using primers for the 5-month-old Tibetan pig sequence which confirmed that the strain belonged to HEV type 4, subtype d (GenBank accession number: OQ981960) and showed 93.30% homology with Sichuan Tibetan pig sequence, MK410044. Bayesian tree analysis showed that the earliest divergence was in 1999 and evidence of homologous recombination was found. Genomic and evolutionary analysis of HEV in the Tibetan Plateau is presented. The importance of continuous surveillance and genomic analysis of HEV is highlighted, especially in regions like the Tibetan Plateau where new strains may emerge. The findings contribute to our understanding of HEV's genetic diversity, evolutionary history and potential risks to animal and human health.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70194"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11745153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence of Cystic Echinococcosis in Sheep and Goats of Ethiopia: A Systematic Review and Meta-Analysis. 埃塞俄比亚绵羊和山羊囊性包虫病患病率:系统回顾和荟萃分析。
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70189
Wakgari Oljira Fayisa, Edilu Jorga Sarba, Bizunesh Mideksa Borena, Endrias Zewdu Gebremedhin, Getachew Kebebew Tola
{"title":"Prevalence of Cystic Echinococcosis in Sheep and Goats of Ethiopia: A Systematic Review and Meta-Analysis.","authors":"Wakgari Oljira Fayisa, Edilu Jorga Sarba, Bizunesh Mideksa Borena, Endrias Zewdu Gebremedhin, Getachew Kebebew Tola","doi":"10.1002/vms3.70189","DOIUrl":"10.1002/vms3.70189","url":null,"abstract":"<p><p>Cystic echinococcosis (CE) is a cestode infection of significant public health and economic importance. A systematic review and meta-analysis was performed to estimate the pooled prevalence of small ruminant CE and associated risk factors in Ethiopia. The published literature written in English from 01 January 2000 to 30 December 2019 was searched from electronic databases and eligible reports that fulfilled inclusion and exclusion criteria. A total of 1140 records were identified, of which 23 reports met the eligibility criteria. A random-effects meta-analysis model was used to pool the prevalence estimations, and a subgroup meta-analysis was utilized to compare the pooled prevalence of CE among the species, age group and sex of the studied animals. The pooled estimated small ruminant CE prevalence was 20.5% (95% confidence interval [CI]: 18.7, 22.3) in Ethiopia, and there was significant heterogeneity among the studies (p < 0.05). The pooled prevalence was higher for sheep, 24.3% (18.5, 30.1), compared to goats, 18.5% (14.5, 22.6), and in adults than young animals. The results of the present analysis suggested a high prevalence of CE at the national level. CE has a significant economic impact due to organ condemnation and production losses and poses a serious public health risk. Effective control requires public awareness, improved slaughterhouse practices, stray dog population management and integrated surveillance and control programmes involving veterinary, public health and environmental sectors. Such synthesis reports are believed to be useful in influencing policymakers towards designing effective prevention and control policies and strategies.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70189"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Elizabethan Collar and Wound Protection Corset on Pain and Discomfort Levels in Cats Undergoing Ovariohysterectomy. 伊丽莎白颈圈和伤口保护胸衣对卵巢子宫切除术猫疼痛和不适程度的影响。
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70180
Ayhan Isil, Latif Emrah Yanmaz
{"title":"Effects of Elizabethan Collar and Wound Protection Corset on Pain and Discomfort Levels in Cats Undergoing Ovariohysterectomy.","authors":"Ayhan Isil, Latif Emrah Yanmaz","doi":"10.1002/vms3.70180","DOIUrl":"10.1002/vms3.70180","url":null,"abstract":"<p><strong>Objective: </strong>To compare the impacts of Elizabethan collar (EC) and wound protection corset (WPC) on pain and discomfort levels in cats following ovariohysterectomy.</p><p><strong>Study design: </strong>Prospective, randomized controlled clinical trial.</p><p><strong>Animals: </strong>Twenty-six healthy female cats.</p><p><strong>Methods: </strong>Animals were randomly assigned to two groups after midline celiotomy: One group wore an EC (n = 13), and the other wore a WPC (n = 13). Pain was evaluated using the Composite Measure Pain Scale-Feline (CMPS-F) and the Multidimensional Pain Scale (MCPS) at intervals of 6, 12, 24, 48, 72 and 96 h, with the requirement for rescue analgesia recorded. Discomfort was assessed through cortisol levels, behavioural observations and the frequency of misbehaviours.</p><p><strong>Results: </strong>No significant differences were observed between groups at all time intervals for CMPS-F and MCPS (p < 0.05). Rescue analgesic administration was similar between the groups, with 16 administrations in the WPC group and 17 in the EC group (p = 0.88). Cortisol levels also showed no significant difference between groups (WPC: 0.05 [0.05-0.3] vs. EC: 0.05 [0.05-0.8]; p = 0.09). Behavioural observations revealed significant differences in misbehaviours, with 10 out of 13 cats in the EC group attempting to remove their collars or exhibiting head shaking (p < 0.01), whereas no misbehaviours were noted in the WPC group.</p><p><strong>Conclusion: </strong>Both EC and WPC provided comparable pain management; however, the significant misbehaviours associated with EC use suggest increased discomfort level.</p><p><strong>Clinical significance: </strong>The WPC may offer a more comfortable recovery experience for cats following ovariohysterectomy, warranting further investigation.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70180"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11720731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Study of Duodenal Histology in Three Lamb Breeds Fed Two Different Rations. 饲喂两种不同口粮的3个羔羊品种十二指肠组织学比较研究。
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70145
Nurcan Delice, Tugrul Ertugrul, Serife Tutuncu, Mustafa Ugurlu, Akif Uysal
{"title":"Comparative Study of Duodenal Histology in Three Lamb Breeds Fed Two Different Rations.","authors":"Nurcan Delice, Tugrul Ertugrul, Serife Tutuncu, Mustafa Ugurlu, Akif Uysal","doi":"10.1002/vms3.70145","DOIUrl":"10.1002/vms3.70145","url":null,"abstract":"<p><strong>Background: </strong>Ration composition may significantly impact the nutrient absorption, duodenal parameters, intestinal health and feed efficiency of animals.</p><p><strong>Objectives: </strong>The objective of this study was to analyse the impact of concentrate- and forage-based diets on essential morphological parameters of the duodenum, including villus height, villus width, crypt depth and goblet cell density, in three different lamb breeds.</p><p><strong>Methods: </strong>Forty-five lambs, aged between 2.5 and 3 months and with an average weight of around 20 kg, were selected. The lambs were fattened on two different diets consisting of pelleted concentrate and alfalfa hay in predetermined ratios: 70% concentrate + 30% forage and 70% forage + 30% concentrate. When the lambs reached 40 kg, they were slaughtered, and duodenum tissue samples were collected for analysis.</p><p><strong>Results: </strong>Histological characteristics were assessed by evaluating duodenal tissues from lambs of three breeds-Akkaraman, Karayaka and Herik-across the two dietary groups within each breed, and the essential parameters of the villus were measured. Akkaraman lambs exhibited lower goblet cell count and villus height (VH) as compared to Karayaka and Herik lambs. Goblet cell count and VH were higher in concentrate-fed lambs, except for Karayaka lambs. However, goblet cell density was similar across Akkaraman, Karayaka and Herik lambs. Herik lambs had higher VH, villus width (VW) and villus surface area as compared to Akkaraman and Karayaka. Villus parameters were generally lower in concentrate-fed lambs, except for Herik lambs.</p><p><strong>Conclusion: </strong>Overall, the study suggests that altering the roughage-concentrate ratio did not significantly affect duodenal mucosa parameters. However, differences were observed across genotypes, particularly in Herik lambs, indicating potential associations between duodenal parameters and genotype.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70145"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11720738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of the Presence of Papillomavirus in Bovine Testicles and Determination of γH2AX and Cytochrome C in Testicular Tissues Determined to be Infected. 牛睾丸乳头瘤病毒存在的调查及检测感染睾丸组织中γ - h2ax和细胞色素C的测定。
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70132
Ozhan Karatas, Mustafa Ozkaraca, Mustafa O Atasoy
{"title":"Investigation of the Presence of Papillomavirus in Bovine Testicles and Determination of γH2AX and Cytochrome C in Testicular Tissues Determined to be Infected.","authors":"Ozhan Karatas, Mustafa Ozkaraca, Mustafa O Atasoy","doi":"10.1002/vms3.70132","DOIUrl":"10.1002/vms3.70132","url":null,"abstract":"<p><p>This study aimed to detect the presence of bovine papillomavirus (BPV) in the testicular tissue of bulls over 1-year old by immunohistochemical, immunofluorescence and molecular assay targeting methods. In addition, γH2AX and cytochrome c expressions were evaluated by immunohistochemical and immunofluorescent methods in samples positive for BPV agent. In this study, 100 testicular specimens that did not show any macroscopic papilloma findings were collected. Testicular tissues were collected and examined using histologically and molecularly methods. In the present study, immunopositivity was detected in 6 (6%) samples with the IHC method and in 11 (11%) samples with the IF method. In addition, BPV positivity was detected in four (4%) samples by the PCR method and four (4%) samples determined to be BPV positive by PCR also showed immunopositivity in IHC and IF methods. γH2AX immunopositivity was seen in two of the BPV-positive samples by the IHC method, and γH2AX immunopositivity was observed in four of the samples determined to be positive for the BPV agent by the IF method. Cytochrome c immunopositivity was found in all the samples determined positive for the BPV agent. When the findings were evaluated, the presence of the BPV agent in the testicular tissues of cattle that did not have macroscopic papillomas was revealed for the first time. It was thought that the BPV agent caused double-stranded DNA breaks and increased cytochrome c expression.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70132"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11720725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Amino Acid Supplementation on the Growth Performance of Broiler Chicks Challenged With Coccidiosis: A Meta-Analysis. 补充氨基酸对球虫病肉鸡生长性能影响的meta分析
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70171
Fatemeh Izadi Yazdanabadi, Gholamali Moghaddam, Mehdi Abbasabadi, Mohsen Akbari, Bahram Satlikh Mohammadi, Parvin Khosravi, Hossein Sabzekar, Reza Farrokhi
{"title":"Effects of Amino Acid Supplementation on the Growth Performance of Broiler Chicks Challenged With Coccidiosis: A Meta-Analysis.","authors":"Fatemeh Izadi Yazdanabadi, Gholamali Moghaddam, Mehdi Abbasabadi, Mohsen Akbari, Bahram Satlikh Mohammadi, Parvin Khosravi, Hossein Sabzekar, Reza Farrokhi","doi":"10.1002/vms3.70171","DOIUrl":"10.1002/vms3.70171","url":null,"abstract":"<p><p>This meta-analysis aimed to evaluate the effects of amino acid supplementation on the growth performance of broiler chicks challenged with coccidiosis. Data were collected on authorship, publication year, study sample characteristics and outcomes of interest related to growth performance. Effect sizes were calculated for both overall effects and the individual effects of each amino acid. The effects of moderator factors, such as supplementation levels and duration, were also assessed, and bias was calculated. Works were obtained from PubMed, Elsevier, ScienceDirect, Wiley, Springer Link and Scopus databases, with the help of search engines like Google Scholar and ResearchGate, covering the period from 2013 to 2023. A random-effects model was used. Thirteen works were included in the current analysis, examining the effects of arginine (n = 4), methionine (n = 6), threonine (n = 3) and glutamine (n = 3). Amino acid supplementation [0.326, CI 95% (0.297-0.355); p = 0.000], arginine [0.430, CI 95% (0.293-0.568); p = 0.000] and threonine [0.793, CI 95% (0.193-1.38); p = 0.009] significantly increased body weight. Amino acid supplementation [0.052, CI 95% (0.022-0.081); p = 0.000] and arginine [0.317, CI 95% (0.049-0.585); p = 0.000] also significantly increased feed intake. Higher levels of amino acids (≥1.50%) and longer supplementation periods (≥15 days) significantly increased body weight and feed intake, while reducing the feed conversion ratio. Funnel plots, fail-safe N and Egger tests showed no evidence of bias. In conclusion, arginine supplementation has the potential to increase body weight and feed intake in poultry challenged with coccidiosis. Higher doses of amino acids and longer supplementation periods are recommended in such cases.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70171"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11662968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Complete Genome Characterization of Canine Adenovirus From Türkiye With Next-Generation Sequencing. 犬<s:1> rkiye腺病毒全基因组测序研究。
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70163
Secil Sevinc Temizkan, Mehmet Cevat Temizkan
{"title":"Complete Genome Characterization of Canine Adenovirus From Türkiye With Next-Generation Sequencing.","authors":"Secil Sevinc Temizkan, Mehmet Cevat Temizkan","doi":"10.1002/vms3.70163","DOIUrl":"10.1002/vms3.70163","url":null,"abstract":"<p><strong>Background: </strong>Determining the complete genome sequence data of adenoviruses has recently become greatly important due to their use by scientists as vectors in cancer studies and other fields, including vaccine development. However, the GenBank database currently has few complete genome sequences of adenoviruses, which are known for their large genomes. To address this gap, we analysed next-generation sequencing data obtained from our previous study to provide the complete genome sequence of the canine adenovirus-2 strain.</p><p><strong>Methods: </strong>For the obtained canine adenovirus-2 strain (OQ596341), comparative genomics, recombination and phylogenetic analysis were conducted. This sequence was compared and phylogenetically analysed with the 20 complete genome sequences of canine adenovirus previously reported in GenBank worldwide, as well as partial E3 ORFA sequences obtained from Türkiye.</p><p><strong>Results: </strong>The nucleotide similarity rates of the sequence obtained from this study with other CAdV-2 whole genomes are over 99.04%. The gene alignment results reveal that the OQ596341 was found to be closely related to the AC000020 reference genome and LC557011. There are two recombination events related to the genome in this study. Comparisons with other complete genome sequences revealed several previously unseen mutations. These mutations include H34Y in the E1A gene; P55A in the E1B 55K gene; D13N and D202N in the IVa2 gene; K679R, V934I and K989N in the Pol gene; E205K in the pTP gene; T455A in the pIIIa gene; A310V in the V gene; G151R in the protease gene; E268K in the 100K gene; G66S and G141S in the 33K gene; T14A, E250K, D287N and I293T in the E3 ORFA gene; and L193F in the E434K gene. Moreover, a comparison with partial sequences obtained from Türkiye revealed the E250K mutation in the E3 ORFA gene, which we report for the first time in Türkiye.</p><p><strong>Conclusions: </strong>The complete CAdV-2 genome sequence obtained in the present study is the first sequence from Europe. Comparative analysis with other genomes revealed some unique mutations. This study is the first to report the E250K amino acid change in the E3 ORFA gene in Türkiye. We anticipate that this data can be used in future CAdV-2 vaccine development studies. Further studies are recommended to evaluate the impact of these mutations on viral tropism and other host interactions.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70163"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluating the In Vitro Anthelmintic Activity of Zinc Oxide Nanoparticles (ZnO-NPs) Against the Adult Stages of Fasciola hepatica. 氧化锌纳米颗粒(ZnO-NPs)对肝片形吸虫成虫的体外驱虫活性评价。
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70170
Mahmoud Abdelhamid, Dalia Fouad, Abdallah Alian, Asmaa A Nasr, H Abd-Allah, Mohamed Farouk, Barakat Shehata Abd Elmaleck, Athanasios Alexiou, Marios Papadakis, Gaber El-Saber Batiha, Fatma A S Anwar
{"title":"Evaluating the In Vitro Anthelmintic Activity of Zinc Oxide Nanoparticles (ZnO-NPs) Against the Adult Stages of Fasciola hepatica.","authors":"Mahmoud Abdelhamid, Dalia Fouad, Abdallah Alian, Asmaa A Nasr, H Abd-Allah, Mohamed Farouk, Barakat Shehata Abd Elmaleck, Athanasios Alexiou, Marios Papadakis, Gaber El-Saber Batiha, Fatma A S Anwar","doi":"10.1002/vms3.70170","DOIUrl":"10.1002/vms3.70170","url":null,"abstract":"<p><strong>Background: </strong>Fascioliasis represents one of the most significant parasitic and foodborne zoonotic diseases in the world. Resistance to currently deployed human and veterinary flukicides is a growing health problem. Zinc oxide nanoparticles (ZnO-NPs) have developed enormous importance in nanomedicine. The current study aims to evaluate the flukicide activity of ZnO-NPs on the adult stages of Fasciola hepatica, in vitro assay.</p><p><strong>Methods: </strong>Mature flukes of F. hepatica were collected from the biliary tracts of cattle liver from the main slaughterhouses in Aswan Governorate, Egypt. The co-precipitation process was utilized to create ZnO-NPs. The adult active flukes of F. hepatica (n = 6) were transferred in Petri dishes (triplicates) containing different concentrations of ZnO-NPs (0.5, 0.10 and 0.12 mg/mL), with simultaneous maintenance of control in only PBS. The treated and control groups of flukes were maintained inside an incubator at 37°C ± 1°C. Mortality and paralysis of the flukes were observed. The adult flukes were picked up for scanning electron microscopy (SEM) examination from the control medium, and those exposed to the highest effective concentration of ZnO-NPs. ZnO-NPs showed dose-dependent flukicide efficacy against the adult flukes.</p><p><strong>Results: </strong>At 0.12 mg/mL concentration of ZnO-NPs, the mortality of flukes was attained early in 4.05 ± 0.033 h. The photomicrographs obtained by SEM of the flukes revealed that ZnO-NPs (0.12 mg/mL) had a potent effect on the integument surface and genital system of F. hepatica. Most of the tegument was damaged with complete erosion and loss of the spines and papillae. Furthermore, a severe rupture in the genital system causes the uterus to burst and releases eggs through the genital pore.</p><p><strong>Conclusions: </strong>Hence, it could be concluded that ZnO-NPs performed effective anthelmintic activity against the adult stages of F. hepatica, in vitro. The results of the present study recommend the use of ZnO-NPs as new preparations with anthelmintic activity.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70170"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Formula Product Injection on Hatching Parameters, Small Intestinal Development and Ileum Histology in Breeder Chicken Eggs. 配方产品注射对种鸡蛋孵化参数、小肠发育及回肠组织学的影响
IF 1.8 3区 农林科学
Veterinary Medicine and Science Pub Date : 2025-01-01 DOI: 10.1002/vms3.70153
Oğuzhan Eray, Gökhan Filik
{"title":"Effects of Formula Product Injection on Hatching Parameters, Small Intestinal Development and Ileum Histology in Breeder Chicken Eggs.","authors":"Oğuzhan Eray, Gökhan Filik","doi":"10.1002/vms3.70153","DOIUrl":"10.1002/vms3.70153","url":null,"abstract":"<p><strong>Background and objective: </strong>This study aimed to determine the effects of in ovo formula product injection on hatching parameters, chick quality, small intestinal development and ileum histology of breeder hen eggs.</p><p><strong>Methods: </strong>A total of 400 fertilised eggs were obtained from the Atak-S parent flock at 42 weeks of age for the experiment. The experiment was designed in two groups: a control group (C), in which no injection was performed, and the other group in which a solution containing formula products at concentrations of 1.25% (F1), 2.5% (F2) and 5% (F3) was injected into 0.5 mL/egg air sac. The hatching rate, embryonic mortality and discard chick rate were examined at the end of the trial.</p><p><strong>Results: </strong>The best result of the hatching rate was found in the Group F2, while there was no difference between the control and Group F1 regarding these parameters. The weight and length of the quality chicks were promoted in the Group F1. In Group F3, the injection of the formula product at a rate of 5% had a negative effect on parameters such as hatchability, embryonic mortality, chick length, Pasgar score and yolk sac weight compared to the other groups. Otherwise, in Group F2, there was a significant increase in villus height, crypt depth and lamina muscularis mucosa thickness compared to the other groups (p < 0.01).</p><p><strong>Conclusions: </strong>As a result, it has been concluded that the appropriate rates for the formula product application on chicks are 1.25% and 2.5%, considering the positive effects of the 1.25% and 2.5% rates and the negative effects of the 5% rate.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70153"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11720732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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