Veterinary Medicine and Science最新文献

{"title":"Effects of Dietary Rumen-Protected Choline on Stress Alleviation, Antioxidant Modulation, and Haematological, Immunological, and Offspring Performance in Transitioning Goats.","authors":"Mohammad Asadi, Mostafa Bokharaeian, Homa Mohammadi Fard","doi":"10.1002/vms3.70524","DOIUrl":"https://doi.org/10.1002/vms3.70524","url":null,"abstract":"<p><p>The transition period is a crucial time for dairy animals, marked by significant physiological changes. This study investigated the effects of supplementing rumen-protected choline (RPC) on oxidative stress, immune function, liver health and performance in periparturient Saanen goats and their offspring. Forty pregnant goats were used, with the research starting 5 weeks pre-partum and continuing until 5 weeks post-partum. The experimental treatments included the following: (1) control-no RPC supplement; (2) choline-treatment receiving 6 g/day of RPC. Blood samples were collected at parturition to analyse antioxidant status, haematology, liver enzymes, immunoglobulins and other parameters. The growth performance of kids was monitored until 30 days of age. RPC increased antioxidant capacity in dams, indicated by higher superoxide dismutase (SOD), catalase (CAT) and total antioxidant status (TAS) compared to control (p < 0.05). RPC also elevated immunoglobulin G and M (IgG and IgM) levels in goats and kids (p < 0.05), though tumour necrosis factor-alpha (TNF-α) and insulin-like growth factor 1 (IGF-1) remained unaffected. Haematological parameters, such as red blood cells (RBCs), haemoglobin (Hb) and haematocrit (HCT), were improved with RPC supplementation, whereas liver enzyme levels remained unchanged. RPC-supplemented kids had greater birth weights and body weights at 10, 20 and 30 days compared to control (p < 0.05). Additionally, average milk intake (AMI) remained constant between both groups (p > 0.05). Overall, RPC demonstrated partial yet promising effects on antioxidant status, immunity and performance in periparturient goats and their kids without affecting liver enzymes. Although some parameters within these domains were not significantly improved, the findings suggest that RPC may contribute to mitigating metabolic stress during the transition period. Further research is needed to clarify its long-term effects on lactation performance and reproductive efficiency.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70524"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12372611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-Related Involutory Changes in Bursa of Fabricius of Anas crecca: Histomorphometric and Ultrastructural Investigation.","authors":"Zeinab Abdel-Fattah, Ahlam Mostafa El-Bakry, Abdelaziz S A Abuelsaad, Ayman M Abdel-Aziz, Ezzat M Awad, Hossam Ebaid, Rasha E H Abo-Eleneen","doi":"10.1002/vms3.70555","DOIUrl":"https://doi.org/10.1002/vms3.70555","url":null,"abstract":"<p><p>Bursa of Fabricius (BF) is a primary lymphoid organ in birds, crucial for lymphocyte development and immune function. This study investigates age-related alterations in the histomorphology and ultrastructure of the bursal tissue in green-winged teal (Anas crecca) at 8^th and 24^th weeks of age. Twenty ducks were examined, and the sample tissues were collected post-euthanasia for histological and scanning electron microscopy (SEM) analyses. At the 8^th week, the histological sections exhibited normal lymphocyte distribution and structural organization, characterized by a well-developed muscular layer and plicae. In contrast, by the 24^th week, significant involution was observed, marked by reduced bursal weight, diminished muscular layer and increased fibrosis. Histological changes included necrosis and depletion of lymphocytes, alongside alterations in the follicular-associated epithelium. SEM revealed collagen fibre spreading and loss of microvilli, indicating advanced involution. This study highlights the developmental and involutionary stages of the organ in A. crecca, providing essential insights into the age-dependent changes in this critical immune organ. This is the first report detailing these histomorphometric and ultrastructural alterations in A. crecca, highlighting the age-dependent nature of these changes, besides contributing to our understanding of avian immunology.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70555"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12366442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Doxorubicin on Cardiac Function in Dogs: Ejection Fraction Changes and Heart Failure Risk.","authors":"Gustavo Cavinato Herrera, Luiz Ricardo Soldi, Leandro Machado Oliveira, Luiz Renato Paranhos, Marcelo José Barbosa Silva","doi":"10.1002/vms3.70497","DOIUrl":"10.1002/vms3.70497","url":null,"abstract":"<p><p>Doxorubicin is an antitumor antibiotic. It is often used in veterinary medicine to treat and extend the lives of dogs with cancer. A cardiotoxic side effect can lead to heart failure and treatment discontinuation. This systematic review and meta-analysis aimed to evaluate the drug's cardiotoxic effects on the ejection fraction (EF) of dogs in doxorubicin protocols. The search was done in eight databases, with a total of 3587 articles screened, resulting in fifteen eligible articles included. A report on the included studies' methods and results was done. It also assessed the risk of bias. Thirteen articles demonstrated cardiac changes in echocardiography with different routes of administration (intravenous and intracoronary). The intracoronary route was more toxic, and in all six studies performed, there was heart failure. The intravenous route caused heart failure in six of the nine studies. A meta-analysis showed this drug worsened heart disease. It included four studies where it significantly lowered the EF. Overall, the intervention produced a mean reduction of 21.24% in EF. This review shows doxorubicin's impact on cardiac function. It reveals the need for careful monitoring of each patient.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70497"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12376308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and Antimicrobial Resistance Profiles of Staphylococcus spp. Strains Isolated From Healthy Stray Dogs.","authors":"Francesca Paola Nocera, Annunziata Romano, Sinem Arslan, Rossana Schena, Francesca Pizzano, Silvia Cappiello, Cristina Di Palma, Barbara Lamagna, Marina Pompameo, Luisa De Martino","doi":"10.1002/vms3.70599","DOIUrl":"10.1002/vms3.70599","url":null,"abstract":"<p><p>The potential role of stray dogs as reservoirs and disseminators of zoonotic antimicrobial-resistant pathogens to humans has long been underestimated. This study aimed to assess the occurrence of antimicrobial-resistant Staphylococcus spp. strains in the ear and nasal swabs collected from stray dogs housed at the Veterinary Hospital of Local Health Authority ASL Napoli 1 Centro. Out of 306 skin samples analysed, 256 bacterial strains were isolated using selective and differential media and identified through MALDI-TOF MS technology. The antimicrobial resistance profiles of the isolated strains were determined using the Kirby-Bauer disc diffusion method on Mueller-Hinton agar, testing susceptibility to 19 antimicrobials across 10 classes. Staphylococcus spp. was isolated in 46% of cases (119/256), with Staphylococcus pseudintermedius being the most prevalent species (50%; 59/119), followed by Staphylococcus simulans (17%; 20/119) and Staphylococcus aureus (14%; 17/119). The evaluation of the antimicrobial resistance profiles revealed a great circulation of methicillin-resistant strains in the stray dog population, as highlighted by the high levels of resistance recorded for penicillin (83.2%; 99/119), cefoxitin (72.3%; 86/119) and oxacillin (62.2%; 74/119). Notably, 85% (101/119) of the isolates were classified as multidrug-resistant, being resistant to three or more antimicrobial classes. These findings suggest that stray dogs may serve as important reservoirs of multidrug-resistant staphylococci, highlighting the need for ongoing surveillance and monitoring of antimicrobial resistance in these animals, especially in the context of the One Health approach that links human, animal and environmental health.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70599"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Study on the Efficacy of Ziziphus vulgaris L. and Camellia sinensis (L.) Kuntze Extracts for Controlling Varroa destructor (Acari: Varroidae) in Apis mellifera (Hym.: Apidae).","authors":"Salim Ojagh, Farnaz Malekifard, Mohammad Yakhchali","doi":"10.1002/vms3.70612","DOIUrl":"10.1002/vms3.70612","url":null,"abstract":"<p><strong>Background: </strong>Ziziphus vulgaris L. and Camellia sinensis (L.) Kuntze are medicinal plants widely used in various ethnomedical systems, particularly in Iranian Traditional Medicine, for the treatment of various diseases.</p><p><strong>Objectives: </strong>While their biological activities are well-documented, their effectiveness against Varroa destructor, a significant ectoparasite of Apis mellifera (honeybees), remains unknown. As V. destructor poses a serious threat to honeybee populations globally, it is crucial for researchers to explore novel control methods.</p><p><strong>Methods: </strong>In this study, we evaluated the anti-Varroa activity of Z. vulgaris and C. sinensis at different concentrations (5, 10, 15, 20 and 25 µL/L air) and exposure times (5, 10, 15, 20 and 25 h).</p><p><strong>Results: </strong>The results showed that mite mortality increased in parallel with the increase in extract concentration and exposure time. Importantly, the plant extracts did not cause significant mortality in A. mellifera compared to the control group.</p><p><strong>Conclusions: </strong>In conclusion, our findings suggest that these plant extracts could be effective agents for managing V. destructor.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70612"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Amyloid A and Haptoglobin as Markers in Cats with Gingivitis-Preliminary Study.","authors":"Ioannis L Oikonomidis, Ioannis Kavarnos, Serafeim Papadimitriou, Jose Joaquin Ceron, Maria Kouki, Katerina K Adamama-Moraitou, Nectarios Soubasis","doi":"10.1002/vms3.70606","DOIUrl":"10.1002/vms3.70606","url":null,"abstract":"<p><strong>Background: </strong>Chronic gingivostomatitis has been associated with increases in α1-acid glycoprotein and serum haptoglobin (Hp) in cats. However, serum amyloid A (SAA) and Hp have not been previously evaluated in cats with uncomplicated gingivitis.</p><p><strong>Objectives: </strong>To compare SAA and Hp between cats with gingivitis and healthy cats, and to investigate the correlation between these two proteins and the severity of gingivitis.</p><p><strong>Methods: </strong>This was a prospective cross-sectional study. Adult, FIV- and FeLV-seronegative cats were included. The cats were allocated into two age- and sex-matched groups. The case group included cats with gingivitis, and the control group included clinically and clinicopathologically healthy cats. The severity of gingivitis was assessed by the Total Mouth Periodontal Score (TMPS)-G index. Serum samples were used to measure SAA and Hp using a previously validated turbidimetric immunoassay and haemoglobin-binding method, respectively. The R statistical language was used for the statistical analysis.</p><p><strong>Results: </strong>A total of 22 cats were included, 11 in each study group. The median (range) age of cats was 5.0 (3.0-11.0) years. The median Hp concentration was significantly higher (p = 0.001) in the case group (2.40 [0.72-4.44] g/L) compared with the control group (1.06 [0.50-1.42] g/L). A significant correlation was found between Hp and TMPS-G (rho = 0.636, p = 0.040). The SAA was below the detection limit (0.4 mg/L) in all samples of the control group and in 10/11 samples of the case group.</p><p><strong>Conclusions: </strong>Feline gingivitis is associated with increased Hp, suggesting the presence of an acute-phase reaction. Haptoglobin appears to be correlated with the severity of the disease.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70606"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rare Case of Systemic Cryptococcal Lymphadenopathy in a Persian Cat.","authors":"Chang-Hyeon Choi, Keon Kim, Chang-Yun Je, Kwang-Jun Lee, Woong-Bin Ro, Chang-Min Lee","doi":"10.1002/vms3.70564","DOIUrl":"10.1002/vms3.70564","url":null,"abstract":"<p><strong>Background: </strong>Cryptococcosis is the most common systemic fungal infection in cats, typically presenting with respiratory or neurological signs. However, cases without these hallmark symptoms are rare and often misdiagnosed. This case is noteworthy for its atypical presentation, where a cat developed systemic cryptococcosis without the typical respiratory or neurological involvement, making it particularly challenging to diagnose. In addition, the overlap of clinical signs with other diseases like lymphoma underscores the importance of considering cryptococcosis in differential diagnoses.</p><p><strong>Case presentation: </strong>A 7-year-old castrated male Persian cat was referred due to generalized cutaneous nodules, including a prominent 4-5 cm nodule on the neck, and generalized lymphadenopathy. Neurological examination revealed an absent menace response in the left eye, raising suspicion of vision loss. Blood tests indicated hyperglobulinemia and the presence of medium-to-large lymphoid cells in the peripheral blood. Diagnostic imaging showed systemic lymphadenopathy, retinal detachment and minimal ascites. Fine needle aspiration of the lymph nodes revealed yeast-like organisms, and culture confirmed Cryptococcus neoformans. Initially treated with itraconazole, the therapy was switched to fluconazole due to better CNS penetration. The lymph node enlargement improved within one week, but further follow-up was limited due to owner constraints.</p><p><strong>Conclusions: </strong>This case emphasizes the diagnostic challenges posed by atypical presentations of feline cryptococcosis, particularly in the absence of respiratory and neurological symptoms. It highlights the importance of considering systemic fungal infections in the differential diagnosis of lymphadenopathy. The overlap with diseases like lymphoma further underscores the need for comprehensive diagnostic workups, including cytology and culture, to ensure accurate diagnosis and effective treatment.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70564"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12402813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Epidemiology and Genetic Diversity of Orientia tsutsugamushi From Patients and Small Mammals in Xiangyun County, Yunnan Province, China.","authors":"Li Chen, Yi-Chen Kong, Jia-Wei Tian, Pei-Yu Han, Song Wu, Chen-Jie He, Ti-Lian Ren, Bo Wang, Lian Qin, Yun-Zhi Zhang","doi":"10.1002/vms3.70573","DOIUrl":"https://doi.org/10.1002/vms3.70573","url":null,"abstract":"<p><strong>Introduction: </strong>Scrub typhus, also known as Tsutsugamushi disease (TD), is an acute febrile illness that poses a significant public health threat to nearly one billion people in the Asia-Pacific region. The disease is caused by Orientia tsutsugamushi (OT) and is primarily transmitted through the bite of chigger mite larvae. Due to its nonspecific clinical manifestations, early diagnosis is challenging, often leading to misdiagnosis or untreated cases. Yunnan Province, located on China's border, reports the highest annual incidence of scrub typhus, highlighting the need to better understand OT infection in both human patients and small mammals in this region.</p><p><strong>Methods: </strong>In this study, clinical data on scrub typhus cases in Xiangyun County People's Hospital were retrospectively collected. Nested polymerase chain reaction (PCR) and semi-nested PCR were used to amplify the 56-kDa type-specific antigen (TSA) from samples collected from 64 patients and 93 small mammals, representing six genera and nine species, in Xiangyun County, Yunnan Province, China. Complete sequences of the 56-kDa TSA for each genotype were obtained through primer-walking and subjected to evolutionary and recombination analyses.</p><p><strong>Results: </strong>A total of 377 scrub typhus cases were enrolled at Xiangyun County Hospital from July 2015 to November 2020. The detection rates of OT in patients and small mammals were 28.13% (18/64) and 13.98% (13/93), respectively. Nine full-length 56-kDa TSA genes were identified, comprising four distinct genotypes: Karp-like (n = 3), Kato-like (n = 3), Gilliam (n = 2), and TA763 (n = 1). Patients infected with the Karp-like exhibited significantly more severe clinical symptoms, and the Karp-like genotype of patients was relatively common in Xiangyun County of Yunnan Province. The Gilliam-like and Karp-like genotypes were detected in the infected small mammals, and Apodemus chevrieri is a dominant species in cultivated fields. Recombination analysis revealed genetic overlap among strains of similar or different genotypes, suggesting that genetic diversity may contribute to the increased incidence of scrub typhus.</p><p><strong>Conclusion: </strong>At least four genotypes of OT were present among patients, and it was infected by OT that at least four species of  infected small mammals in cultivated areas and at least three species of infected small mammals in residential areas from Xiangyun County, Yunnan Province. The diversity of OT genotypes and infected small mammals, which causes frequent genetic recombination, is the main reason for the adaptive selection due to the genetic evolution of OT.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70573"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12366444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in Rats.","authors":"Mürşide Ayşe Demirel, İpek Süntar, Ali Osman Çeribaşı, Kevser Taban, Gökhan Zengin","doi":"10.1002/vms3.70582","DOIUrl":"https://doi.org/10.1002/vms3.70582","url":null,"abstract":"<p><p>Pseudopregnancy is a luteal phase syndrome characterized by symptoms of late pregnancy and/or early postpartum periods. This phenomenon is observed in various species, including rats, rabbits and dogs, and arises due to hormonal alterations. Due to the significant side effects of current treatment regimens, there is a growing need for the development of alternative therapeutic strategies. In this context, various plants and natural compounds have been investigated for their potential to modulate hormonal balance, offering a promising avenue for the treatment of pseudopregnancy. Artemisia absinthium L., a medicinal plant traditionally utilized as an emmenagogue and abortifacient, is known for its regulatory effect on the dopaminergic system. The study aimed to assess whether A. absinthium treatment could restore oestrous cyclicity in pseudopregnant rats. Pseudopregnancy was induced through the administration of a pregnant mare's serum gonadotropin and human chorionic gonadotropin in female rats. The essential oil of A. absinthium was administered orally at doses of 12.5, 25 and 50 mg/kg once daily for 10 days, with bromocriptine as a reference treatment. The results indicated that the 25 mg/kg dose of A. absinthium essential oil exhibited beneficial effects in the pseudopregnancy model. Gas chromatography analysis revealed that the major components of the essential oil included cis-chrysanthenyl acetate (17.8%), sabinyl acetate (11.6%), terpinen-4-ol (6.2%), caryophyllene oxide (5.5%) and (E)-nuciferol (5.5%). These findings suggest that A. absinthium essential oil may present a promising therapeutic option for the management of pseudopregnancy.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70582"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12372613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Population Structure and Selection Signatures in Two Iraqi Fat-Tailed Sheep Breeds.","authors":"Mervan Bayraktar, Omer Shoshin, K A Saravanan, Tahreer M Al-Thuwaini, Gökhan Gökçe, Zeynab Hussein Fadhil, Melis Çelik Güney","doi":"10.1002/vms3.70584","DOIUrl":"https://doi.org/10.1002/vms3.70584","url":null,"abstract":"<p><p>This study aimed to elucidate the genetic architecture and selection signatures in two prominent Iraqi fat-tailed sheep breeds, Awassi and Hamdani. One hundred forty animals were genotyped using the Illumina Ovine SNP50K BeadChip, providing genome-wide coverage with approximately 53,714 SNP markers. To uncover candidate genomic regions under selection, we employed three complementary approaches: integrated haplotype score (iHS), runs of homozygosity (ROH) and Tajima's D analyses. The iHS analysis identified 69 candidate genes in Awassi sheep and 286 in Hamdani sheep, whereas ROH analysis revealed 190 and 277 candidate genes in the respective breeds. Tajima's D corroborated these findings with one gene in Awassi and four in Hamdani sheep. Population structure was rigorously assessed using ADMIXTURE, principal component analysis (PCA) and neighbour-joining phylogenetic tree reconstruction, collectively demonstrating a distinct genetic separation of the Awassi breed and a more admixed genetic profile for the Hamdani breed. Functional enrichment analysis of candidate genes implicated several biological processes and pathways, including immune response, hormone regulation and cellular signalling, underscoring their potential roles in adaptation and disease resistance. Overall, our findings provide novel insights into the genetic differentiation and adaptive evolution of Iraqi fat-tailed sheep, offering a valuable resource for future breeding and conservation programmes.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 5","pages":"e70584"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}