Veterinary Medicine and Science最新文献

Molecular and Seroprevalence of Mycoplasma gallisepticum in Turkeys in Sylhet District of Bangladesh.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70227

Jahid Hasan Tipu, Rijon Miah, Obaidul Islam, Md Mukidur Rahman, Lucky Talukdar, Rubel Miah, Md Safwan Hussain, Md Ashraful Islam, Md Irtija Ahsan, Ahsan Raquib, Monira Noor

{"title":"Molecular and Seroprevalence of Mycoplasma gallisepticum in Turkeys in Sylhet District of Bangladesh.","authors":"Jahid Hasan Tipu, Rijon Miah, Obaidul Islam, Md Mukidur Rahman, Lucky Talukdar, Rubel Miah, Md Safwan Hussain, Md Ashraful Islam, Md Irtija Ahsan, Ahsan Raquib, Monira Noor","doi":"10.1002/vms3.70227","DOIUrl":"10.1002/vms3.70227","url":null,"abstract":"Mycoplasma gallisepticum (MG) poses a significant threat to Bangladesh's poultry industry, causing substantial economic losses every year. This study aimed to determine the prevalence of MG infection in turkeys using serum plate agglutination (SPA), enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in Sylhet, Bangladesh from December 2019 to November 2020. In addition, we evaluated the diagnostic accuracy of these tests and identified potential risk factors associated with MG infection. A total of 250 blood samples and 250 tracheal swabs were collected from suspected turkeys across 25 farms from three sub-districts of Sylhet namely Sylhet Sadar, Golapganj and Beanibazar. Blood samples were tested with SPA and ELISA, while tracheal swabs were analysed by PCR targeting the 16S rRNA gene of MG. The overall prevalence of MG was 35.2%, 29.2% and 25.6% for SPA, ELISA and PCR respectively. Higher infection rates were observed in turkeys aged 0-4 months (SPA 57.1%, ELISA 52%, PCR 42.8%), during winter (SPA 43.1%, ELISA 37.8%, PCR 30%) and among female turkeys (SPA 54.5%, ELISA 49.5%, PCR 45.5%). Geographically, the Beanibazar had the highest prevalence (SPA 54.2%, ELISA 48.6%, PCR 41.4%), compared to the Sylhet Sadar and Golapganj sub-districts. Both SPA and ELISA tests showed 100% sensitivity, with specificity of 87.1% and 95.2%, respectively using PCR as a gold standard. Overall, these findings provide valuable insights for developing effective control measures for MG infections in the poultry industry of Bangladesh.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70227"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TNFRSF1A and NCF1 May Act as Hub Genes in Mastitis.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70278

Mohammad Soleiman Ekhtiyari, Maryam Yousefi, Farhad Samadian, Mostafa Ghaderi-Zefrehei, Saied Neysi, Javad Shirani Shamsabadi, Arash Javanmard, Hamed Shahriarpour, Bluma Lesch

{"title":"TNFRSF1A and NCF1 May Act as Hub Genes in Mastitis.","authors":"Mohammad Soleiman Ekhtiyari, Maryam Yousefi, Farhad Samadian, Mostafa Ghaderi-Zefrehei, Saied Neysi, Javad Shirani Shamsabadi, Arash Javanmard, Hamed Shahriarpour, Bluma Lesch","doi":"10.1002/vms3.70278","DOIUrl":"10.1002/vms3.70278","url":null,"abstract":"Background: Bovine mastitis (BM) is a major source of morbidity among cattle, imposing a significant burden on the dairy industry. Understanding the molecular mechanisms that confer susceptibility to bovine mastitis offers potential for therapeutic targeting, but these mechanisms remain complex and incompletely understood.Objectives: This study aims to identify key molecular players and regulatory networks involved in bovine mastitis by integrating multiple expression datasets and applying network analysis.Methods: We integrated data from three BM expression datasets and applied weighted gene co-expression network analysis (WGCNA) to identify specific modules and hub genes related to BM. Within the module that best correlated with mastitis, we constructed a network of mRNA-miRNA and protein-protein interactions.Results: Our analysis revealed that TNFRSF1A and NCF1 are implicated as hub genes in the host's immune response to BM. Additionally, we identified bta-mir-2881 as a potential regulator of both TNFRSF1A and NCF1. These genes are crucial in regulating the immune response and defence against bacterial infections, highlighting their importance in mastitis susceptibility.Conclusion: Identifying miRNAs like bta-mir-2881 that may target key genes involved in mastitis susceptibility can shed light on the molecular mechanisms underlying the disease. This provides new targets for BM prevention and treatment, potentially alleviating the significant burden of the disease on the dairy industry.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70278"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Calcium Carbonate Particle Size, Phytase and Midnight Feeding on Performance, Egg and Bone Quality and Blood Parameters in Laying Hens.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70248

Vahid Salehi, Reza Vakili, Mahdi Elahi Torshizi

{"title":"Effects of Calcium Carbonate Particle Size, Phytase and Midnight Feeding on Performance, Egg and Bone Quality and Blood Parameters in Laying Hens.","authors":"Vahid Salehi, Reza Vakili, Mahdi Elahi Torshizi","doi":"10.1002/vms3.70248","DOIUrl":"10.1002/vms3.70248","url":null,"abstract":"Background: To achieve maximum production in laying hens, calcium (Ca) and phosphorus (P) are two important minerals in the diet. Nutritional solutions such as adding coarser Ca particles in the afternoon or considering the light hours during the night to feed the birds might improve the eggshell quality and reduce Ca mobilization from the bone.Objectives: This experiment aimed to study the effect of calcium carbonate particle size (CCps), phytase (Pht) and midnight feeding (MF) on the performance, egg and bone quality and blood parameters in laying hens from 60 to 70 weeks of age.Methods: A total of 720 Hy-line (W-36) laying hens were arranged in a factorial arrangement of 2 × 2 × 2 based on a completely randomized design with two types of CCps (100% fine and 75% coarse + 25% fine), two levels of Pht (0 and 300 FTU/kg) and two states of feeding (MF and without MF). The experimental period lasted 12 weeks from 60 to 70 weeks of age.Results: During the trial, the significant impact of CCps on the feed conversion ratio (FCR) and breaking strength (BS) of the tibia was obtained (p < 0.05). FCR, eggshell percentage and the percentage of tibia ash, Ca and P were affected by Pht supplementation (p < 0.05). MF affected egg weight (EW), eggshell percentage and serum concentration of Ca, P and alkaline phosphatase (ALP) (p < 0.05). The interaction effects of CCps and Pht were significant for Ca level in serum (p < 0.05).Conclusions: In general, the use of Pht in the diets improved the FCR and quality of eggshell and tibia, and diets containing Pht and coarser CCps increased blood Ca levels. Despite the improvement of egg quality by MF, serum parameters showed a significant decrease.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70248"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergence Retraction Nystagmus Secondary to Central Nervous System Disseminated Coccidioides Immitis Infection in a Dog.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70249

Lydia Lovato, Trevor Moore, Laura Stainback, Scott B Plummer, Kim E Knowles, Jaime Sage

{"title":"Convergence Retraction Nystagmus Secondary to Central Nervous System Disseminated Coccidioides Immitis Infection in a Dog.","authors":"Lydia Lovato, Trevor Moore, Laura Stainback, Scott B Plummer, Kim E Knowles, Jaime Sage","doi":"10.1002/vms3.70249","DOIUrl":"10.1002/vms3.70249","url":null,"abstract":"This report describes a Belgian Malinois dog residing in metropolitan Phoenix, Arizona that was presented to a veterinary neurology center with abnormal eye movements as his only clinical sign. On examination, clinical signs were isolated to convergence-retraction nystagmus. A brain MRI was performed which identified two well-demarcated, T2-hypointense and T1-isointense, avidly contrast enhancing mass lesions with moderate to severe perilesional T2 and FLAIR hyperintensity within the left frontal lobe and left dorsal midbrain. Imaging characteristics were most consistent with Coccidioides immitis fungal granulomas and serology was supportive of Coccidioidomycosis infection. The patient's clinical signs resolved quickly with antifungal and anti-inflammatory therapies, and brain granulomas were resolved or resolving on repeat imaging 10 months after initial diagnosis. To the authors' knowledge, this is the first report of convergence-retraction nystagmus secondary to an infectious etiology described in a canine patient.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70249"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucose Transport by Follicle-Stimulating Hormone Is Mediated Through the Akt/FOXO1 Pathway in Ovine Granulosa Cells.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70294

Xin Xu, Ruotong Wang, Linlin Pei, Quanfeng Wang, Chunjie Liu

{"title":"Glucose Transport by Follicle-Stimulating Hormone Is Mediated Through the Akt/FOXO1 Pathway in Ovine Granulosa Cells.","authors":"Xin Xu, Ruotong Wang, Linlin Pei, Quanfeng Wang, Chunjie Liu","doi":"10.1002/vms3.70294","DOIUrl":"10.1002/vms3.70294","url":null,"abstract":"Glycolysis in granulosa cells (GCs) is the primary location of energy metabolism and its substrates in oocytes and is closely related to follicular development in mammals. The complex morphological structure and physiological functions of GCs are regulated by follicle-stimulating hormone (FSH), but little is known about how FSH regulates glycolysis in GCs, and its mechanism remains unclear. The purpose of this study is to investigate the mechanism by which FSH activates the Akt/FOXO1 pathway, thereby regulating glucose metabolism in ovine GCs. Granulosa cells were cultured in the presence of different concentrations of FSH and a CCK-8 assay was used to measure the proliferation of the treated cells. Next, qRT-PCR was performed to measure the transcription of the target genes, glucose transporter (GLUT). Western-blot analysis of the phospho-Akt and -FOXO1 levels induced by FSH through the glucose signalling pathway, in addition to the effects of these proteins on the expression levels of the downstream GLUT genes, was measured. Results showed that the addition of 10ng/mL FSH to the culture medium increased the viability of granulosa cells. Transcription of GLUT1 and 4 was significantly up-regulated in FSH-treated cells through activation of the Akt/FOXO1 phosphorylation pathway, thereby affecting glucose metabolism. This study contributes to the current understanding of the metabolic features of and the associated developmental pathways of ovine follicular GCs.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70294"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromeria congesta Alleviates LPS-Induced Inflammation, Apoptosis, Oxidative Stress and DNA Damage in Rat Heart and Kidneys.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70264

Muhammet Bahaeddin Dörtbudak, Muhammed Demircioğlu, Fuat Serkan Kapucuk

{"title":"Micromeria congesta Alleviates LPS-Induced Inflammation, Apoptosis, Oxidative Stress and DNA Damage in Rat Heart and Kidneys.","authors":"Muhammet Bahaeddin Dörtbudak, Muhammed Demircioğlu, Fuat Serkan Kapucuk","doi":"10.1002/vms3.70264","DOIUrl":"10.1002/vms3.70264","url":null,"abstract":"Life-threatening sepsis with high mortality and morbidity is an important cause of acute kidney injury and myocardial dysfunction. In this study, we investigated the protective effect of Micromeria congesta (MC) against kidney and heart damage caused by lipopolysaccharide (LPS) used as a sepsis model. Control, LPS, LPS + 25 mg/kg MC and LPS + 50 mg/kg MC groups were established from rats for the study. After the experiment, kidney and heart tissues obtained from the rats were stained with hematoxylin-eosin for histopathologic examination. Immunohistochemical staining was performed to determine inflammation, apoptosis, oxidative stress and DNA damage. IL-2 for inflammation, CASP-3 for apoptosis, HSP-27 for oxidative stress and 8-OHdG for DNA damage were used for immunopathologic examination. Histopathologic examination showed that the lesions in the kidney and heart tissues in the LPS group decreased with increasing doses of MC. Immunohistochemical examination showed that the expression of IL-2, CASP-3, HSP-27 and 8-OHdG was severe in the LPS group, but the severity of expression in these tissues decreased with increasing doses of MC. As a result of the study, it was histopathologically determined that MC reduced LPS-induced kidney and heart tissue damage. In addition, MC was found to protect against LPS by reducing LPS-induced inflammation, apoptosis, oxidative stress and DNA damage in kidney and heart tissue. In conclusion, it was seen that MC was effective in sepsis damage. However, it was concluded that MC could be an alternative in drug strategies developed for sepsis treatment with studies in vivo including more analyses.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70264"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multivariate Approach to Antimicrobial Residue Concentrations in Animal-Derived Products: An Analytical Review.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70297

Juan Carlos Angeles-Hernandez, David Alejandro Contreras Caro Del Castillo, Astrid Espinosa-Sánchez, Lizbeth E Robles-Jimenez, Navid Ghavipanje, Manuel Gonzalez-Ronquillo

{"title":"Multivariate Approach to Antimicrobial Residue Concentrations in Animal-Derived Products: An Analytical Review.","authors":"Juan Carlos Angeles-Hernandez, David Alejandro Contreras Caro Del Castillo, Astrid Espinosa-Sánchez, Lizbeth E Robles-Jimenez, Navid Ghavipanje, Manuel Gonzalez-Ronquillo","doi":"10.1002/vms3.70297","DOIUrl":"10.1002/vms3.70297","url":null,"abstract":"Background: Antimicrobial resistance (AMR) represents an alarming global public health concern exacerbated by livestock antibiotic misuse, affecting humans and the environment. However, the precise magnitude of antimicrobial residue concentrations in animal-derived products remains not well understood. This study aimed to quantify antimicrobial residues in animal products through an analytical literature review.Methods: This review covered the scientific articles from 1977 to 2020. The antimicrobials were classified according to the European Medicines Agency (EMA) guidelines into four categories. The final database comprised seven qualitative variables (antibiotic, antibiotic class, region, country, decade, EMA category, animal product and animal species) and one quantitative variable (residue concentration recorded as µg/kg). Due to the number of variables involved in the study, a multivariate analysis approach was used using a Factor Analysis of Mixed Data (FAMD) carried out in R.Results: The highest concentrations of antimicrobial residues were detected in fish samples, followed by egg. Notably, concentrations of ruminant-derived products were lower than to monogastric. β-Lactam was the most prevalent residue followed by aminoglycosides, sulphonamides and quinolones, respectively. Moreover, South America had the highest residues levels, followed by Asia and Europe.Conclusions: The multivariate analysis reveals a possible association between the EMA category, animal species, antimicrobial class and animal product. In conclusion, the concentration of antimicrobial residues in products of animal origin depends mainly on their origin (product, species and geographic region), showing the highest concentrations in products derived from fish and poultry.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70297"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of Milbemycin Oxime in Pekingese Dogs after Single Oral and Intravenous Administration.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70312

Ze-En Li, Ming-Hui Duan, Yan Dai, Yang-Guang Jin, Yue Liu, Yan-Ni Zhang, Xing-Ping Li, Fan Yang

{"title":"Pharmacokinetics of Milbemycin Oxime in Pekingese Dogs after Single Oral and Intravenous Administration.","authors":"Ze-En Li, Ming-Hui Duan, Yan Dai, Yang-Guang Jin, Yue Liu, Yan-Ni Zhang, Xing-Ping Li, Fan Yang","doi":"10.1002/vms3.70312","DOIUrl":"10.1002/vms3.70312","url":null,"abstract":"Objective: This study aimed to characterize the pharmacokinetic profiles of milbemycin oxime in Pekingese dogs following a single oral (PO) and intravenous (IV) dose. Six clinically healthy Pekingese dogs, with an average body weight (BW) of 4.75 kg, were included. Each dog received an IV injection of milbemycin oxime solution and PO doses of both milbemycin oxime tablets and nanoemulsion, all administered at 1 mg/kg BW.Methods: Blood samples (∼0.6 mL) were collected at various time points, and milbemycin oxime concentrations were measured using a validated high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection. Pharmacokinetic parameters were obtained through non-compartmental analysis (NCA) using WinNonLin software.Results: Oral administration of milbemycin oxime tablets resulted in a peak concentration (Cmax) of 0.33 ± 0.07 µg/mL at 2.47 ± 1.90 h, with a mean residence time (MRT) of 21.96 ± 14.43 h and an absolute bioavailability of 51.44% ± 21.76%. In contrast, the nanoemulsion achieved a significantly higher Cmax of 8.87 ± 1.88 µg/mL, with a much quicker time to peak concentration (Tmax) at 0.33 ± 0.13 h, an MRT of 21.74 ± 18.21 h, and an absolute bioavailability of 99.26% ± 12.14%. After IV administration, total clearance (Cl) and steady-state volume of distribution (VSS) were 0.13 ± 0.06 mL/kg/h and 2.36 ± 0.73 mL/kg, respectively.Conclusions: These findings demonstrate that the milbemycin oxime nanoemulsion is absorbed more rapidly and completely, with significantly higher bioavailability than the tablet form. This suggests that the nanoemulsion could effectively overcome the issues of poor diffusion and low bioavailability associated with tablet formulations, positioning it as a promising alternative to traditional milbemycin oxime tablets.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70312"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Immune-Related Responses of Several Slow-Growing Indigenous Chickens With a Fast-Growing Broiler in Iran.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70288

Donya Khazali, Alireza Ehsani, Ali Akbar Masoudi, Mohammad Amir Karimi Torshizi, Rasoul Vaez Torshizi, Peymaneh Davoodi, Alireza Valizadeh, Seyed Mohammad Ali Sheykh-Al-Eslami

{"title":"Comparison of Immune-Related Responses of Several Slow-Growing Indigenous Chickens With a Fast-Growing Broiler in Iran.","authors":"Donya Khazali, Alireza Ehsani, Ali Akbar Masoudi, Mohammad Amir Karimi Torshizi, Rasoul Vaez Torshizi, Peymaneh Davoodi, Alireza Valizadeh, Seyed Mohammad Ali Sheykh-Al-Eslami","doi":"10.1002/vms3.70288","DOIUrl":"10.1002/vms3.70288","url":null,"abstract":"Relationship between immunity and growth rate in chickens is complex and multifaceted. Chicken's immune system plays a crucial role in its overall health, well-being and ability to grow and develop properly. On the other hand, indigenous chickens (ICs) are slow growing but considered to have higher immunocompetence compared to fast-growing commercial broilers. Therefore, this study aimed to evaluate immune responses to sheep red blood cell (SRBC) and phytohaemagglutinin (PHA) in slow-growing chickens, including five different Iranian ecotype ICs and a fast-growing broiler of Ross 308 (R). A total of 600 chicks, 100 from each sex and strain, were raised under identical environmental and management conditions after hatching eggs from five distinct Iranian ICs from West Azerbaijan (W), Khorasan (K), Fars (F), Isfahan (I), Mazandaran (M) and a commercial Ross 308 were collected. To investigate humoral immune responses, two SRBC injections were administered at ages 53 and 60 days, and at 67 days, the haemagglutination method was used to assess immunoglobulin Y (IgY), immunoglobulin M (IgM) and total immunoglobulin (IgT). Additionally, a commercial kit and microplate reader were used to assess the amounts of total protein and albumin/globulin. Cellular immune responses were evaluated by administering a PHA injection. Mainly SRBC responses and plasma protein levels in ICs were higher than R. The PHA responses of R and W were higher than other ICs (p < 0.05). These significant differences may confirm that the immunocompetence of ICs are higher than that in the commercial lines.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70288"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Nuclear (Ef1α) and Mitochondrial (mt-CO1 and mt-Nad5) Markers for Molecular Characterization of Sheep Isolates of Echinococcus granulosus sensu lato.

IF 1.8 3区农林科学

Veterinary Medicine and Science Pub Date : 2025-03-01 DOI: 10.1002/vms3.70313

Seyma Gunyakti Kilinc, Harun Kaya Kesik, Figen Celik, Sami Simsek

{"title":"Comparative Analysis of Nuclear (Ef1α) and Mitochondrial (mt-CO1 and mt-Nad5) Markers for Molecular Characterization of Sheep Isolates of Echinococcus granulosus sensu lato.","authors":"Seyma Gunyakti Kilinc, Harun Kaya Kesik, Figen Celik, Sami Simsek","doi":"10.1002/vms3.70313","DOIUrl":"10.1002/vms3.70313","url":null,"abstract":"Background: Echinococcus granulosus is a zoonotic disease that is widespread worldwide.Objective: This study aimed to determine the genetic diversity of E. granulosus isolates in sheep. Partial mitochondrial and nuclear gene sequences were used to determine intraspecific variation.Methods: For this purpose, 41 sheep hydatid cyst isolates were collected from slaughterhouses in Elazig and Bingol provinces of Türkiye. Following genomic DNA isolation from the germinal membrane of the cysts, PCR amplification and subsequent DNA sequence analysis were performed using primers that amplify mitochondrial CO1, Nad5, and nuclear ef1α gene regions.Results: DNA sequence analyses of mt-CO1 (1603 bp) and mt-Nad5 (625 bp) gene regions showed that 39 out of 41 isolates were identified as G1 and two isolates as G3. When the phylogenies formed by the sequences belonging to different gene regions were evaluated; in the phylogenetic tree created with the mt-CO1 and Nad5 gene dataset, G1 and G3 isolates were in separate clades with the reference sequences, while in the phylogenetic tree created with the nuclear gene region ef1α dataset, the isolate belonging to the G3 genotype (ef1a.01) formed a sister clade with the G1 genotypes and the reference sequence. According to the haplotype network results obtained, 14 haplotypes, 15 haplotypes, and two haplotypes were determined for mt-CO1, mt-Nad5, and ef1α gene regions, respectively. Haplotype analysis of mt-CO1 and mt-Nad5 gene regions revealed high haplotype and low nucleotide diversity. Low nucleotide diversity was detected, and two haplotypes were determined as a result of haplotype analysis ef1α (1343 bp) gene.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70313"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0