Veterinary Medicine and Science最新文献

{"title":"FABP4 Mediates Inflammation to Regulate Endometrial Epithelial Cell Function During Early Gestation in Sheep.","authors":"Yan Chen, Chang Liu, Zhijiao Liu, Hengbin Yu, Xiayu Peng, Jing Wang, Guangdong Hu","doi":"10.1002/vms3.70193","DOIUrl":"10.1002/vms3.70193","url":null,"abstract":"<p><p>The objective of this experiment was to understand the impact of fatty acid binding protein 4 (FABP4)-mediated inflammation on sheep endometrial epithelial cells (sEECs). To model the hormonal environment of sheep during pregnancy, estradiol (E₂), progesterone (P₄) and interferon-tau (IFN-τ) were used. The sEECs were then stimulated with different concentrations of lipopolysaccharide (LPS) to determine suitable concentrations for inducing inflammation, while FABP4 expression was inhibited using a FABP4-specific inhibitor. The expression of genes related to inflammation, cell apoptosis, cell proliferation, endometrial tolerance, and embryo development were detected by real-time quantitative PCR and Western blot. The cell proliferation ability was detected by CCK-8, and the cell migration ability was tested by scratch assay. The results showed that LPS-induced inflammation inhibited the expression levels of proliferation genes, migration ability genes and endometrial tolerance-related genes and enhanced the expression levels of apoptosis genes and gestational elongation-related genes and proteins in the sEECs. The use of FABP4 inhibitors reduced LPS-induced inflammation and alleviated the inhibitive effect of LPS on cellular function. The experiments showed that inhibiting the expression of the FABP4 gene reduced LPS-induced inflammation and alleviated the impact of LPS on the viability of sEECs.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70193"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disease Prevalence, Usage of Aquaculture Medicinal Products and Their Sustainable Alternatives in Freshwater Aquaculture of North-Central Bangladesh.","authors":"Md Nure Rasul, Tahmid Hossain, Sifat-Un-Nuri, Md Nurul Haider, Muhammad Tofazzal Hossain, Md Shaheed Reza","doi":"10.1002/vms3.70276","DOIUrl":"10.1002/vms3.70276","url":null,"abstract":"<p><strong>Background: </strong>The use and abuse of aquaculture medicinal products (AMPs) has increased throughout the globe to enhance fish production and maximize profits. This study aimed to investigate the status of disease prevalence and usage of AMPs in aquaculture operations in North-Central Bangladesh, a region famous for its significant carp and catfish production within the Asian subcontinent. Farmers in this area are recognized for their innovative approaches to aquaculture.</p><p><strong>Methods: </strong>Data were collected through pretested questionnaire interviews and participatory rural appraisal (PRA) tools.</p><p><strong>Results: </strong>Among the 90 surveyed grow-out farmers, the majority practiced mixed cultures of carps, Nile tilapia and catfish (primarily Pangasius) where diseases such as epizootic ulcerative syndrome (EUS), saprolegniasis, streptococcosis, tail and fin rot and bacillary necrosis were common. Despite the widespread use of AMPs, their efficacy is often limited, and their overuse can contribute to the development of antibiotic-resistant bacteria and environmental pollution. Additionally, the extra-label drug use (ELDU) has become an issue that results in controversial application of drugs by fish farmers. All together 142 AMPs of different groups were marketed by 24 companies, including antibiotics, disinfectants, nutritional supplements, probiotics, gas remover and salt. Among these, 3 groups of antibiotics with 11 active ingredients were identified, sold under 17 trade names in drug retail outlets. These antibiotics, originally intended for veterinary or poultry use, were often misapplied in aquaculture, raising concerns about ELDU practices. Due to the limited efficacy of many of these antibiotics, farmers adopted alternative approaches such as in-pond probiotics and fermented products. It was revealed that 12% (11 out of 90) of the farmers were using these alternatives that led to approximately 20% approved fish growth with a cost reduction up to 30% compared to the conventional culture cycles. Farmers also reported a reduction in disease outbreaks in ponds managed with these alternatives.</p><p><strong>Conclusions: </strong>Promotion of sustainable and responsible practices is essential for the long-term growth of aquaculture industry. It is expected that the use of probiotics and fermented products can reduce the reliance on conventional AMPs, thereby contributing to enhanced food security and economic development in Bangladesh.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70276"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Willingness to Vaccinate Animals, Pay for Vaccination and Its Contributing Factors in Negele-Arsi, Shashemene and Hawassa, Ethiopia.","authors":"Alazar Mekonnen Teffera, Yoseph Cherinet Megerssa","doi":"10.1002/vms3.70301","DOIUrl":"10.1002/vms3.70301","url":null,"abstract":"<p><strong>Introduction: </strong>In veterinary medicine, vaccines help in combating various infectious diseases, thus maintaining the health and welfare of the animals. However, in Ethiopia, the use of vaccines has been hindered by multiple challenges, and the extent of willingness to vaccinate (WTV) has not been properly assessed.</p><p><strong>Methods: </strong>A cross-sectional study was carried out from December 2023 to February 2024 to assess the willingness of livestock owners to vaccinate and the factors affecting it in Negele-Arsi, Shashemene and Hawassa, Ethiopia. A total of 100 questionnaire samples (50 from Negele-Arsi, 30 from Shashemene and 20 from Hawassa) were collected from veterinary clinics in these areas.</p><p><strong>Results: </strong>Overall, 38% of the respondents reported WTV for their animals, 36% of the reported willingness to pay, whereas 75% of the respondents also chose drugs as a control method for diseases compared to vaccines. Age, educational level of livestock owners and the rate of importance of vaccines were also shown to have a significant association with WTV (p < 0.05). As challenges, livestock owners mentioned that inaccessibility, high price of vaccines and harm caused by vaccines as the major obstacles they face when vaccinating their animals.</p><p><strong>Conclusion: </strong>This study has demonstrated that the lack of awareness and knowledge about vaccines, coupled with challenges livestock owners are facing, are the obstacles hindering the vaccination of a vast number of livestock in the three areas. Therefore, it is necessary to address these problems properly to ensure the effective delivery of vaccines for the appropriate service of vaccines.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70301"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ebselen Alleviates Sepsis-Induced Acute Kidney Injury by Regulating Endoplasmic Reticulum Stress, Apoptosis, and Oxidative Stress.","authors":"İhsan Karaboğa, Hamza Malik Okuyan, Serdar Doğan, Şeyda Öznur Ayçiçek, Hüseyin Çakıroğlu","doi":"10.1002/vms3.70318","DOIUrl":"10.1002/vms3.70318","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is one of the most serious complications of sepsis, with substantial morbidity and mortality, and no effective treatment exists. Ebselen is of pharmacological significance in the treatment and prevention of a variety of human diseases, such as cancer and cardiovascular disorders. Nevertheless, the role of Ebselen in the pathogenesis of sepsis-induced AKI remains unknown. Therefore, we aimed to elucidate the impact of Ebselen, an active seleno-organic compound, on AKI induced by lipopolysaccharide (LPS) and the associated molecular mechanisms, including endoplasmic reticulum (ER) stress, apoptosis, and oxidative stress. We established the sepsis-induced AKI rat model by injecting 5 mg/kg of LPS intraperitoneally. The rats were given Ebselen (5 and 10 mg/kg, orally) before receiving the LPS injection. Ebselen treatment alleviated renal tubular injury and reduced the levels of blood urea nitrogen (BUN) and creatinine (CREA) in LPS-induced sepsis model. Immunohistochemical and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) analyses revealed that Ebselen reduced caspase-3 expressions and apoptotic cells triggered by LPS in kidney tissues. LPS-induced sepsis caused ER stress, and Ebselen treatment alleviated the ER stress by regulating eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) and GRP78 in kidney tissue, as well as activating transcription factor 4 (ATF4) and activating transcription factor 6 (ATF6) in serum. Ebselen decreased malondialdehyde (MDA) levels induced by LPS. Ebselen alleviated LPS-induced oxidative stress by modulating MDA and superoxide dismutase (SOD) levels in kidney tissues and SOD, glutathione peroxidase (GPx) and serum total antioxidant status (TAS) levels in serum. In conclusion, we report for the time that Ebselen might alleviate sepsis-induced AKI through the regulation of ER stress apoptosis and oxidative stress.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70318"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spondylosis in Horses: Clinical Features, Diagnostic Imaging Findings, Treatment and Outcome in 13 Horses.","authors":"Claudia de Secondi, Federica Cantatore, Marco Marcatili, Marianna Biggi, Jonathan Withers, Donatella de Zani, Davide Zani","doi":"10.1002/vms3.70196","DOIUrl":"10.1002/vms3.70196","url":null,"abstract":"<p><strong>Background: </strong>Back pain is a debilitating condition hampering horses' athletic careers. Thoracic spondylosis (TS), a known cause of back pain, leads to osteophytes formation across intervertebral joints. In horses, TS is poorly reported, with anecdotal signs and response to treatment.</p><p><strong>Objectives: </strong>To report clinical presentation, diagnostic imaging findings, treatment and outcome in horses with TS.</p><p><strong>Methods: </strong>The clinical records of horses diagnosed with TS between 2010 and 2023 were reviewed. Signalment, clinical and imaging findings, treatment, and outcome were analysed. Thoracic spondylosis was graded from 1 to 5. Grades, concurrent pathologies, treatment, and outcome were assessed. The median TS grade and number of lesions and outcome were compared using the Mann-Whitney test.</p><p><strong>Results: </strong>Thirteen horses met inclusion criteria, eight of which performed a discipline involving jumping. All horses exhibited signs consistent with back pain. Thoracic spondylosis sites varied from 1 to 6 (mean 2) with a total of 32 lesions. The most affected site was T13-T14. Five horses had concurrent dorsal spinous processes impingement and three were lame. Treatment included physiotherapy, tiludronate, anti-inflammatory and extracorporeal shockwave therapy. Long-term follow-up (>12 months) was available for 11 horses: the outcome was poor in seven horses, good in one and excellent in three. No statistically significant association was found between TS grade (p = 0.4), number of lesions (p = 0.2) and outcome categories.</p><p><strong>Conclusions: </strong>Although rare, TS can cause back pain. The outcome is generally poor, but some horses may continue athletic activity despite severe lesions.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 2","pages":"e70196"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples.","authors":"Md Sadequl Islam, Md Ahsan Habib, Nasrin Sultana Tonu, Md Samiul Haque, Md Mostafizer Rahman","doi":"10.1002/vms3.70200","DOIUrl":"10.1002/vms3.70200","url":null,"abstract":"<p><strong>Background: </strong>Brucellosis is a zoonotic disease caused by Brucella spp., affecting various animals and humans, leading to significant economic and public health impacts. Traditional diagnostic methods, mainly serological, often fail to detect seronegative carriers, which continue to spread the infection.</p><p><strong>Objective: </strong>This review aims to highlight advancements in molecular diagnostics that address these limitations.</p><p><strong>Methods: </strong>A systematic search of PubMed, Web of Science and Scopus was conducted, focusing on studies using seronegative, PCR, qPCR and biosensor-based techniques. Data extraction and meta-analyses were performed, evaluating pooled detection rates and heterogeneity.</p><p><strong>Results: </strong>Through analysis of existing studies, we review key molecular techniques, including PCR, LAMP and biosensor-based assays, which offer high sensitivity and specificity by detecting bacterial DNA directly, thus overcoming the challenges of antibody-based tests. Meta-analysis of detection rates across different studies showed significant variability, with rates ranging from 0.96% to 100%, highlighting differences in sample types, animal species and regions. The pooled detection proportion from random-effects models was 35.08%, indicating that many seronegative animals still carry Brucella spp. A forest plot analysis further confirmed heterogeneity in detection, underlining the importance of using molecular diagnostics alongside serological tests to identify hidden carriers.</p><p><strong>Conclusion: </strong>Innovations like nanoparticle-enhanced biosensors and CRISPR-Cas systems show promise for rapid, on-site diagnostics. The findings suggest that integrating molecular methods with traditional serology can improve surveillance and disease management. Future research should focus on developing portable, field-ready diagnostic devices and standardised protocols, along with exploring novel biomarkers to detect latent infections. A collaborative One Health approach, involving veterinary, public health and environmental sectors, is essential for comprehensive disease control and eradication efforts.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70200"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Breeding for Short-Tailedness in Sheep on Parameters of Reproduction and Lamb Development.","authors":"Hannah Hümmelchen, Henrik Wagner, Kerstin Brügemann, Sven König, Axel Wehrend","doi":"10.1002/vms3.70138","DOIUrl":"10.1002/vms3.70138","url":null,"abstract":"<p><strong>Background: </strong>Sheep's tail docking is a widespread practice, which is banned or critically discussed in some countries to improve animal welfare.</p><p><strong>Objective: </strong>The aim was to determine the influence of breeding for short-tailedness (ST) or long-tailedness (LT) in sheep on the development of reproduction parameters and lamb performance.</p><p><strong>Method: </strong>One hundred forty-nine ewes were mated with four rams according to tail length. Pregnancy and pregnancy loss rates were calculated. During pregnancy, the progesterone levels (P4) of the ewes were measured. The weight and length of the 254 lambs were recorded up to 14 weeks of life. Litter size, placenta weight, sex, stillbirths, vitality, morbidity and mortality of the lambs were also documented.</p><p><strong>Results: </strong>No significant differences were found for P4 and placental weight for the two mating groups (ST and LT). Although the pregnancy rate for ST was slightly lower (75.71%) than for LT (87.34%), there was only a low significant difference (p = 0.07). The sex distribution was 61 (48.80%) male and 64 (51.20%) female lambs in the ST group and 67 (51.94%) male and 62 (48.06%) female lambs in the LT group. The twinning rate was not significantly different (ST 75.20%; LT 75.97%), and no significant difference was found in the average body length and vitality of the lambs. However, LT lambs showed better weight gains that were marginally significant (p = 0.09).</p><p><strong>Conclusion: </strong>This study found no evidence that reproductive parameters or lamb performance were affected by selective breeding based on tail length.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70138"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portulaca oleracea Extract Ameliorates Testosterone Propionate-Induced Benign Prostatic Hyperplasia in Male Sprague-Dawley Rats.","authors":"Young-Ju Lim, Hye Rim Kim, Seul Bi Lee, Sang Back Kim, Dong-Hee Kim, Jae-Hyun So, Kyung-Ku Kang, Soo-Eun Sung, Joo-Hee Choi, Minkyoung Sung, Yeon-Ji Lee, Wook-Tae Park, Gun Woo Lee, Seul-Ki Kim, Min-Soo Seo","doi":"10.1002/vms3.70184","DOIUrl":"10.1002/vms3.70184","url":null,"abstract":"<p><p>Benign prostatic hyperplasia (BPH) is a distressing health problem that can cause serious complications in aging men. Androgens are implicated in the causation of BPH. Portulaca oleracea (PO) is a natural product with diverse pharmacological effects. The objective of this study was to investigate the effect of PO in a rat model of testosterone propionate (TP)-induced BPH and explore the underlying mechanisms. Thirty-five Sprague-Dawley (SD) rats were divided into the following equal groups (n = 7): normal control (NC) group, TP (3 mg/kg) group, finasteride (10 mg/kg) group, 25 and 50 mg/kg PO groups. At the end of the experiment, the body weights (BWs) of the rats were measured before they were euthanized to the establishment obtain serum and prostate weight (PW). TP-induced levels of androgen-related proteins in the prostate were also investigated. In the TP group, prostate size, BW, serum DHT level, prostate epithelial cell thickness and androgen-related protein level were higher than those in the NC group (p < 0.001). PO reversed TP-induced BPH in a dose-dependent manner (p < 0.01) and its effect was similar to that of finasteride. A similar effect of PO on the androgen-related protein level was also observed. We successfully established a TP-induced BPH rat model. This is the first study to demonstrate that inhibition of androgen-related proteins using PO can alleviate BPH.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70184"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of Parasitic Fauna in Semi-Scavenging Indigenous Chickens (Gallus gallus domesticus) in Bangladesh.","authors":"Kausar-A-Noor, Md Mehadi Hasan, Anisuzzaman, Mohammad Zahangir Alam, Mst Sawda Khatun, Anita Rani Dey","doi":"10.1002/vms3.70211","DOIUrl":"10.1002/vms3.70211","url":null,"abstract":"<p><strong>Background: </strong>Parasites are a major concern for profitable poultry production worldwide as they impede the health, welfare and production performance of poultry.</p><p><strong>Objectives: </strong>The present study was designed to detect the diversity of parasitic fauna and associated factors of gastrointestinal (GI) helminths and lice in indigenous chickens.</p><p><strong>Methods: </strong>A total of 310 indigenous chickens were collected from different villages at Gauripur and Mymensingh Sadar, Mymensingh, and Bangladesh, and various parasites were identified.</p><p><strong>Results: </strong>Out of 310 indigenous semi-scavenging chickens, 281 were infected with one or more species of helminths with an overall prevalence of 90.6%. The identified species of helminths were Ascaridia galli (60.6%), Heterakis gallinarum (29.0%) and Cheilospirura hamulosa (14.2%), Catatropis verrucosa (7.7%), Echinostoma revolutum (7.4%), Raillietina spp. (76.5%) and Hymenolepis spp. (5.8%). The prevalence of lice infestations was 74.2%, and identified species were Menopon gallinae (72.6%), Goniodes gigas (11.6%) and Lipeurus caponis (10.3%). Co-infections with helminths were 65.8% and with lice were 19.4% in chickens. Univariate analysis was performed to measure the association between predictor variables and parasitic infections by considering several biotic and abiotic variables, including age, sex, flock size, farming nature, use of anthelmintic/insecticides and socio-economic status of owners. No significant (p < 0.05) variation was found in helminth infections but large flock size (87.0%) and mixed farming nature (81.2%) were significantly associated with lice infestations in chickens.</p><p><strong>Conclusions: </strong>Awareness related to the management system of chickens rearing need to be increased for formulating control strategy against parasitic infections in indigenous chickens in Bangladesh.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70211"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovine Toxoplasmosis Sero-Status, Risk Factors Analysis, and Estimation of Haematological and Serum Biochemical Alterations.","authors":"Shadan Hassan Abdullah, Hiewa Othman Dyary, Omer Ismael Dana, Mohamad Hasanvand","doi":"10.1002/vms3.70220","DOIUrl":"10.1002/vms3.70220","url":null,"abstract":"<p><p>Toxoplasmosis is a significant food-borne protozoal disease in humans and animals. The study aimed to find out Toxoplasma seropositivity in sheep, estimate epidemiological risk factors and assess haemato-biochemical parameter changes. Blood samples were collected from 276 indigenous sheep in five districts surrounding Sulaymaniyah Province in the northern region of Iraq. Toxoplasmosis was determined by detecting anti-Toxoplasma antibodies by ELISA. The impact of various risk factors on seropositivity was evaluated. Haematological and serum biochemical changes from naturally infected sheep were analysed using commercial kits. The overall seroprevalence of Toxoplasma antibodies among the studied population was 41.67%. Sharazoor district had a higher prevalence rate of 46.15% than Bakrajo, with the lowest frequency rate of 38.18%. Ewes with an abortion history had a higher risk for seropositivity of about 47.4% (OR = 1.4, 95% CI: 0.9-1.6). Sampling season and herd size are other risk factors, with higher seropositivity at 42.5% (OR = 1.1, 95% CI: 0.8-1.4) during the dry season and among small flocks ≤ 100 animals/farm at 43.6% (OR = 1.1, 95% CI: 0.7-1.4). Toxoplasma prevalence was correlated with animals' ages; a higher seropositivity of 43.3% was found among older sheep (OR = 0.8, 95% CI: 0.6-1.2). Seropositive sheep had significantly higher total leukocyte, lymphocyte and granulocyte counts but significantly lower haemoglobin, haematocrit and total erythrocyte counts. The serum levels of total protein and albumin were significantly reduced, with increased blood urea nitrogen and creatinine concentrations. Also, AST enzyme activity represents a higher value in positive sheep with toxoplasmosis. Toxoplasma infection among the ovine population is common. Its impact on livestock productivity should not be neglected. A potential risk for humans through infected sheep could be a hazardous source for public health.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 1","pages":"e70220"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}