肉仔鸡肉及食用内脏中耐甲氧西林金黄色葡萄球菌(MRSA)肠毒素流行及耐药性研究

IF 1.7 3区 农林科学 Q2 VETERINARY SCIENCES
Mousavi Bideli Seyedeh Mozhgan, Rahimi Ebrahim, Seyed Majid Hashemi, Zia Jahromi Noosha
{"title":"肉仔鸡肉及食用内脏中耐甲氧西林金黄色葡萄球菌(MRSA)肠毒素流行及耐药性研究","authors":"Mousavi Bideli Seyedeh Mozhgan, Rahimi Ebrahim, Seyed Majid Hashemi, Zia Jahromi Noosha","doi":"10.1002/vms3.70413","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The responsible for staph infection is methicillin-resistant Staphylococcus aureus (MRSA) which has a long and difficult treatment process due to resistance to this type of antibiotic. This study is designed to investigate the distribution and frequency of antibiotic-resistant genes and MRSA enterotoxins isolated from the meat and edible viscera of broiler chickens, which are responsible for pathogenicity in humans.</p><p><strong>Materials and methods: </strong>A total of 523 meat and edible viscera of broiler chicken collected from farms in Shahrekord, Iran. The antibiogram test of 142 MRSA isolates was performed by Kirby-Bauer diffusion disc. Sensitivity or resistance of MRSA was tested on 13 different antibiotics. DNA extracted from MRSA was screened by PCR technique for the presence of antibiotic-resistant and enterotoxin genes.</p><p><strong>Results: </strong>Staphylococcus aureus (S. aureus) isolated with frequency of 51.05% (267 of 523). The presence of mecA gene in S. aureus was examined to detect the MRSA. The most antibiotic-resistance responsible genes and the pathogenic enterotoxin genes were identified. MRSA was identified by positive amplification of mecA in 53.18% (142 of 267) of S. aureus isolates. S. aureus antimicrobial resistance was most frequently noted against tetracycline (94.37%), ampicillin (88.73%) and penicillin (71.83%). Out of 51 examined isolates, 47 isolates exhibited the sea (92.15%), and 7 isolates exhibited the sej (13.72%).</p><p><strong>Conclusion: </strong>The results indicated high prevalence of MRSA in broilers, which is very worrying issue. In addition, in the present study, it was observed that due to the increase in the use of antibiotics in poultry farming, bacteria resistant to methicillin and other antibiotics have a high prevalence. Now, with the knowledge that the consumption of broiler chicken is very high all over the world and with the increasing trend of antibiotic consumption, this issue has become a concern at the global health level. The presence of antibiotic-resistance and enterotoxigenic genes in MRSA bacteria is a critical threat to human nutrition, making consuming contaminated meat and edible viscera of broiler chickens unsafe.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 4","pages":"e70413"},"PeriodicalIF":1.7000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231201/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigating the Prevalence of Enterotoxin and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Meat and Edible Viscera of Broiler Chickens.\",\"authors\":\"Mousavi Bideli Seyedeh Mozhgan, Rahimi Ebrahim, Seyed Majid Hashemi, Zia Jahromi Noosha\",\"doi\":\"10.1002/vms3.70413\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The responsible for staph infection is methicillin-resistant Staphylococcus aureus (MRSA) which has a long and difficult treatment process due to resistance to this type of antibiotic. This study is designed to investigate the distribution and frequency of antibiotic-resistant genes and MRSA enterotoxins isolated from the meat and edible viscera of broiler chickens, which are responsible for pathogenicity in humans.</p><p><strong>Materials and methods: </strong>A total of 523 meat and edible viscera of broiler chicken collected from farms in Shahrekord, Iran. The antibiogram test of 142 MRSA isolates was performed by Kirby-Bauer diffusion disc. Sensitivity or resistance of MRSA was tested on 13 different antibiotics. DNA extracted from MRSA was screened by PCR technique for the presence of antibiotic-resistant and enterotoxin genes.</p><p><strong>Results: </strong>Staphylococcus aureus (S. aureus) isolated with frequency of 51.05% (267 of 523). The presence of mecA gene in S. aureus was examined to detect the MRSA. The most antibiotic-resistance responsible genes and the pathogenic enterotoxin genes were identified. MRSA was identified by positive amplification of mecA in 53.18% (142 of 267) of S. aureus isolates. S. aureus antimicrobial resistance was most frequently noted against tetracycline (94.37%), ampicillin (88.73%) and penicillin (71.83%). Out of 51 examined isolates, 47 isolates exhibited the sea (92.15%), and 7 isolates exhibited the sej (13.72%).</p><p><strong>Conclusion: </strong>The results indicated high prevalence of MRSA in broilers, which is very worrying issue. In addition, in the present study, it was observed that due to the increase in the use of antibiotics in poultry farming, bacteria resistant to methicillin and other antibiotics have a high prevalence. Now, with the knowledge that the consumption of broiler chicken is very high all over the world and with the increasing trend of antibiotic consumption, this issue has become a concern at the global health level. The presence of antibiotic-resistance and enterotoxigenic genes in MRSA bacteria is a critical threat to human nutrition, making consuming contaminated meat and edible viscera of broiler chickens unsafe.</p>\",\"PeriodicalId\":23543,\"journal\":{\"name\":\"Veterinary Medicine and Science\",\"volume\":\"11 4\",\"pages\":\"e70413\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231201/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Medicine and Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1002/vms3.70413\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medicine and Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/vms3.70413","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

背景:葡萄球菌感染的原因是耐甲氧西林金黄色葡萄球菌(MRSA),由于对这类抗生素的耐药性,其治疗过程漫长而困难。本研究旨在调查从肉鸡肉和可食用内脏中分离的耐药基因和MRSA肠道毒素在人类中的致病性分布和频率。材料和方法:从伊朗Shahrekord农场采集的肉鸡肉和可食用内脏共523只。采用Kirby-Bauer扩散盘对142株MRSA进行抗生素谱试验。对13种不同的抗生素进行了MRSA的敏感性或耐药性测试。从MRSA中提取的DNA采用PCR技术筛选耐药基因和肠毒素基因。结果:金黄色葡萄球菌(S. aureus)分离率为51.05%(523例中有267例)。检测金黄色葡萄球菌中mecA基因的存在,以检测MRSA。鉴定出主要的耐药基因和致病性肠毒素基因。267株金黄色葡萄球菌中142株(53.18%)经mecA阳性扩增检出MRSA。金黄色葡萄球菌对四环素(94.37%)、氨苄西林(88.73%)和青霉素(71.83%)耐药最多。51株分离株中,有47株(92.15%)表现为海型,7株(13.72%)表现为sej型。结论:MRSA在肉鸡中流行率高,值得关注。此外,在本研究中观察到,由于家禽养殖中抗生素使用的增加,对甲氧西林和其他抗生素耐药的细菌患病率很高。现在,随着世界各地肉鸡消费量的增加和抗生素消费量的增加,这一问题已成为全球健康层面关注的问题。MRSA细菌中抗生素耐药和产肠毒素基因的存在对人类营养构成严重威胁,使食用受污染的肉鸡肉和可食用内脏变得不安全。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Investigating the Prevalence of Enterotoxin and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Meat and Edible Viscera of Broiler Chickens.

Investigating the Prevalence of Enterotoxin and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Meat and Edible Viscera of Broiler Chickens.

Investigating the Prevalence of Enterotoxin and Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Meat and Edible Viscera of Broiler Chickens.

Background: The responsible for staph infection is methicillin-resistant Staphylococcus aureus (MRSA) which has a long and difficult treatment process due to resistance to this type of antibiotic. This study is designed to investigate the distribution and frequency of antibiotic-resistant genes and MRSA enterotoxins isolated from the meat and edible viscera of broiler chickens, which are responsible for pathogenicity in humans.

Materials and methods: A total of 523 meat and edible viscera of broiler chicken collected from farms in Shahrekord, Iran. The antibiogram test of 142 MRSA isolates was performed by Kirby-Bauer diffusion disc. Sensitivity or resistance of MRSA was tested on 13 different antibiotics. DNA extracted from MRSA was screened by PCR technique for the presence of antibiotic-resistant and enterotoxin genes.

Results: Staphylococcus aureus (S. aureus) isolated with frequency of 51.05% (267 of 523). The presence of mecA gene in S. aureus was examined to detect the MRSA. The most antibiotic-resistance responsible genes and the pathogenic enterotoxin genes were identified. MRSA was identified by positive amplification of mecA in 53.18% (142 of 267) of S. aureus isolates. S. aureus antimicrobial resistance was most frequently noted against tetracycline (94.37%), ampicillin (88.73%) and penicillin (71.83%). Out of 51 examined isolates, 47 isolates exhibited the sea (92.15%), and 7 isolates exhibited the sej (13.72%).

Conclusion: The results indicated high prevalence of MRSA in broilers, which is very worrying issue. In addition, in the present study, it was observed that due to the increase in the use of antibiotics in poultry farming, bacteria resistant to methicillin and other antibiotics have a high prevalence. Now, with the knowledge that the consumption of broiler chicken is very high all over the world and with the increasing trend of antibiotic consumption, this issue has become a concern at the global health level. The presence of antibiotic-resistance and enterotoxigenic genes in MRSA bacteria is a critical threat to human nutrition, making consuming contaminated meat and edible viscera of broiler chickens unsafe.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinary Medicine and Science
Veterinary Medicine and Science Veterinary-General Veterinary
CiteScore
3.00
自引率
0.00%
发文量
296
期刊介绍: Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信