Veterinary Medicine and Science最新文献

{"title":"Identification of SNPs Associated With mRNA Expression Differences of the Complement Receptor Type 1-Like Gene in Landrace Weaned Piglets.","authors":"Wei Yin, Jiachen Cheng, Qiongyu Li, Luyang Xu, Hongquan Li, Kuohai Fan, Zhenbiao Zhang, Na Sun, Panpan Sun, Huizhen Yang","doi":"10.1002/vms3.70980","DOIUrl":"10.1002/vms3.70980","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate the association between single nucleotide polymorphisms (SNPs) of porcine complement receptor 1-like (CR1-like) gene and its mRNA expression.</p><p><strong>Methods: </strong>SNP identification, qRT-PCR absolute quantification, linkage disequilibrium analysis and dual-luciferase reporter assay were performed using 392 forty-day-old Landrace weaned piglets.</p><p><strong>Results: </strong>28 SNPs were identified, 4 loci were significantly correlated with CR1-like expression (p < 0.05). The c.-1372GA mutation led to the loss of GATA-1 binding site, and significantly reduced promoter transcriptional activity (< P0.01).</p><p><strong>Conclusion: </strong>CR1-like gene polymorphisms regulate its expression via disrupting transcription factor binding, which provides a theoretical basis for porcine disease-resistant breeding.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e70980"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13238697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148043964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Vitex pseudo-negundo in a Bovine Mastitis Model Experimentally Induced by Staphylococcus aureus in Rats.","authors":"Hüseyin Koç, Arzu Fındık, Tolga Güvenç, Nevzat Batan, Seda Fandaklı, Murat Fındık","doi":"10.1002/vms3.71031","DOIUrl":"10.1002/vms3.71031","url":null,"abstract":"<p><strong>Background: </strong>Mastitis is one of the most prevalent and economically significant diseases in dairy cattle, causing to inflammation of the mammary gland and reduced milk yield.</p><p><strong>Objectives: </strong>This study investigated the therapeutic efficacy of Vitex pseudo-negundo (VPN) extracts in an experimentally induced rat mastitis model.</p><p><strong>Methods: </strong>Sixty female Wistar rats were randomly assigned into six groups of 10. Group I served as the control group. Mastitis was induced by intramammary injection of Staphylococcus aureus (SA) (1.5 × 10⁸ cfu/mL; 10 µL per gland) into the L4, L5, R4 and R5 mammary glands. Group III received intramammary gentamicin (0.25 mg/10 µL/day) for 5 days. Groups IV-VI received VPN extracts-water (0.2603 µg/kg/day), ethanol (0.0803 µg/kg/day) and petroleum ether (0.1042 µg/kg/day), administered at 10 µL per gland for the same duration. Rats were clinically monitored three times daily from the onset of clinical signs until euthanasia.</p><p><strong>Result: </strong>SA infection significantly increased mammary gland weight, total oxidative status (TOS) and bacterial load and induced tissue pathology, while decreasing total antioxidant status (TAS). C-reactive protein levels remained unchanged. VPN significantly improved mammary gland weight, TOS, TAS, bacterial load and histological parameters (p < 0.001). Mammary gland weight, oxidative stress, bacterial load and histopathological lesion severity all decreased markedly following treatment. Histological examination showed near-normal restoration of mammary gland architecture in all treatment groups.</p><p><strong>Conclusion: </strong>Preparations of VPN, particularly the water extract, demonstrated strong potential for restoring altered microbiological, biochemical and histological parameters in this rat mastitis model.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71031"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multispecies Systematic and Critical Review of Intranasal Administration in Veterinary Anaesthesia and Emergency Care: Promising Evidence and Overlooked Challenges.","authors":"Majid Jafarbeglou","doi":"10.1002/vms3.71041","DOIUrl":"10.1002/vms3.71041","url":null,"abstract":"<p><p>Intranasal (IN) drug delivery has increasingly considered as an easy, practical and non-invasive alternative to parenteral administration in veterinary medicine, offering rapid systemic and potential nose-to-brain effects. The first part of this review systematically collected and synthesized published evidence on IN administration across animal species, while the second part critically analysed the anatomical, pharmacological and technical factors that determine its success and limitations. Part I consisted a total of 110 eligible studies published between 1991 and 2025, encompassing dogs, cats, rabbits, pigs, ruminants, birds and reptiles. IN delivery has been investigated for a range of purposes and produced clinically meaningful sedation, analgesia and drug reversal, often comparable to intramuscular administration but generally characterized by slower onset and greater variability among species. Despite encouraging and favourable results, IN delivery was not without limitations. Its effectiveness can be strongly influenced by species-specific nasal anatomy and physiology, formulation characteristics and dosing volume. Defensive reactions, poor tolerability, sneezing, nasopharyngeal irritations, hypersalivation or swallowing of the drug are frequently reported. Future progress requires species-specific case selection guidelines and dosing standards, pharmacokinetic validation and developing safe concentrated formulations. Transparent reporting and balanced assessment of both benefits and drawbacks are essential to ensure the safe, effective and ethically responsible integration of IN administration into veterinary anaesthesia and critical care practice.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71041"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13278371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148279026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Variation and Health Risk Assessment of Aflatoxin M1 in Milk From the Central Region of Iran.","authors":"Pedram Hashemi, Mohammadhosein Movassaghghazani, Milica Zekovic","doi":"10.1002/vms3.71099","DOIUrl":"10.1002/vms3.71099","url":null,"abstract":"<p><strong>Background: </strong>Aflatoxin M1 (AFM1) contamination in milk is a major public health concern due to its carcinogenicity and resistance to processing.</p><p><strong>Objectives: </strong>This study assessed seasonal variation in AFM1 levels across raw, pasteurized and ultra-high-temperature (UHT) milk and quantified associated non-carcinogenic and carcinogenic risks.</p><p><strong>Methods: </strong>A total of 180 samples were collected over four seasons (October 2024-2025), and AFM1 concentrations were measured using HPLC-fluorescence detector (FLD). Distributional characteristics were described using medians and 95th percentile (P95) values, and prevalence estimates were reported with 95% confidence intervals. A health risk assessment was conducted using the estimated daily intake (EDI), hazard index (HI) and margin of exposure (MOE).</p><p><strong>Results and conclusions: </strong>AFM1 was detected in all samples (6.10-176.20 ng/kg). Overall, 23.33% (95% CI: 17.7%-30.1%) exceeded the Iranian limit (100 ng/kg), and 58.88% (95% CI: 51.6%-65.8%) surpassed the EU limit (50 ng/kg). Seasonal analysis showed substantially higher contamination during warm months, with raw milk in summer presenting the highest upper tail concentration (P95: 168.57 ng/kg). Risk assessment based on P95 exposure indicated medium non-carcinogenic risk (HI: 1-10) for children across all milk types and for adults consuming raw milk. Carcinogenic risk characterization showed MOE values below 10000 for children consuming raw and pasteurized milk, indicating inadequate safety margins. These findings demonstrate that reliance on mean AFM1 concentrations underestimates health risks, especially for children. The pronounced seasonal patterns highlight the need for strengthened monitoring and targeted control strategies, with priority given to raw milk during warm seasons to protect vulnerable paediatric populations in Iran.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71099"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13384337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148537129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of Multidrug-Resistant Salmonella spp. in Raw Meat Retailed in Wet Markets in Chattogram City, Bangladesh.","authors":"Mukta Das Gupta, Arup Sen, Abdul Ahad, Plabon Ketan Barua, Partha Paul, Uaye Mya, Snigdha Das, Ashutosh Das","doi":"10.1002/vms3.71071","DOIUrl":"10.1002/vms3.71071","url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial resistance (AMR) in foodborne pathogens has become a global public health issue. Salmonella is one of the most widespread foodborne pathogens worldwide, causing foodborne-related hospitalizations and deaths. Multidrug resistance (MDR) in Salmonella makes foodborne salmonellosis challenging to treat and control. Foodborne salmonellosis often follows the consumption of contaminated animal products, typically resulting from the use of infected animals in food production or contamination of carcasses or edible organs. Therefore, screening raw meats for MDR in Salmonella spp. can help identify the extent of exposure to antimicrobial resistance through food. This study aimed to determine the prevalence and molecular identification of the isolates, as well as to screen them for the presence of three antimicrobial resistance genes: blaTEM, sul1, and sul2.</p><p><strong>Methods: </strong>A total of 200 raw meat samples (beef, n = 50; goat, n = 50; buffalo, n = 50; and chicken, n = 50) were collected from five local wet markets in Chattogram city, Bangladesh. Salmonella spp. was phenotypically detected following ISO-6579-1 guidelines and biochemical tests. For molecular identification, polymerase chain reaction (PCR) was performed using Salmonella-specific paired primers (ST11 and ST15). The antimicrobial susceptibility profiles of PCR-positive Salmonella isolates were determined by the disk diffusion method. The isolates were also screened for three antimicrobial resistance genes: blaTEM, sul1, and sul2.</p><p><strong>Results: </strong>The overall prevalence of Salmonella spp. in raw meat was 13.5% (95% CI 9.03-19.03), with the highest detection rate in poultry meat. All 27 phenotypically positive isolates were confirmed as Salmonella in the molecular screening of PCR. All the Salmonella isolates were found to be MDR with the highest resistance to sulfamethoxazole-trimethoprim (100%), amoxicillin (92.6%), and ampicillin (92.6%). The most prevalent resistance genotype was blaTEM (81.5%), whereas 11.1% and 18.5% of the Salmonella isolates were of the sul1 and sul2 genotypes, respectively.</p><p><strong>Conclusion: </strong>The present study revealed that a substantial proportion of Salmonella isolates from raw meat showed resistance to multiple antibiotics, posing potential public health concerns in Chattogram city, Bangladesh. One Health interventions are necessary to mitigate the spread of MDR Salmonella.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71071"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13325551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148368967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Betaine Promotes Milk Protein Synthesis via Alleviating Oxidative Stress Caused by NEFA in Mammary Epithelial Cells of Dairy Cows.","authors":"Wenyan Yan, Xinyuan Sun, Huanjie Shi, Zhenwang Li, Wenhui Li, Xiaoxiao Gao, Jihong Dong","doi":"10.1002/vms3.71065","DOIUrl":"10.1002/vms3.71065","url":null,"abstract":"<p><strong>Background: </strong>Fatty liver is highly prevalent in dairy cows and poses a significant challenge to the dairy industry by reducing both milk yield and quality, thereby inflicting substantial economic losses. Although dietary betaine supplementation has been demonstrated to enhance milk protein content, the underlying molecular mechanisms remain to be fully elucidated.</p><p><strong>Objectives: </strong>This study aimed to elucidate the role of betaine in milk protein synthesis and the underlying mechanisms.</p><p><strong>Methods: </strong>Blood, liver and mammary gland samples were obtained from healthy and fatty liver-affected bovines. The serum NEFA levels, oxidation and antioxidant enzyme systems and milk protein synthesis-related proteins expression were determined. In vitro, the bovine mammary epithelial cells (BMECs) were pretreated with 25 mM betaine/10 mM N-acetyl-L-cysteine (NAC) and then stimulated with NEFA.</p><p><strong>Results: </strong>The results indicated an elevation in serum NEFA levels accompanied by enhanced oxidative stress (OS) and reduced protein levels related to milk protein synthesis (phosphorylated (p)-mechanistic target of rapamycin (mTOR)/mTOR, p-ribosomal protein S6 kinase 1 (S6K1)/S6K1, p-Janus kinase 2 (JAK2)/JAK2, p-signal transducer and activator of transcription 5 (STAT5)/STAT5 and β-casein) in dairy cows with fatty liver. In vitro, the results revealed betaine decreased the hydrogen peroxide (H₂O₂), malondialdehyde (MDA), and oxygen free radicals (OFR) contents, and lactate dehydrogenase (LDH) activity in the supernatant, but increased the glutathione peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD) and thioredoxin reductase (TrxR) activities; the reduced glutathione-to-oxidised glutathione (GSH/GSSG) ratio; and the total antioxidant capacity (T-AOC); and elevated protein levels related to milk protein synthesis in NEFA-treated BMECs.</p><p><strong>Conclusions: </strong>These findings indicate that betaine reduces NEFA-induced OS and enhances milk protein synthesis, suggesting its potential as a nutritional intervention for enhancing milk protein content in dairy cows with metabolic disorders.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71065"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148340359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D CT Anatomy and Morphology of the Appendicular Skeleton of European Pond Turtle (Emys orbicularis).","authors":"Omid Zehtabvar, Ali Reza Vajhi, Somaye Davudypoor, Roshanak Mokhtari, Kiana Farahani, Seyyed Hossein Modarres Tonekabony","doi":"10.1002/vms3.71019","DOIUrl":"10.1002/vms3.71019","url":null,"abstract":"<p><strong>Background: </strong>Turtles have special anatomical features due to their shell and the enclosure of their internal organs. One of the important differences between the anatomy of this group of reptiles and others is the skeletal structure. The European pond turtle (Emys orbicularis) is a species within the Emylidae family, inhabiting regions spanning Europe, Africa and Asia.</p><p><strong>Objective: </strong>This study has been designed to assess the anatomical features of the appendicular skeletal system in this species and draw comparisons with other turtle species.</p><p><strong>Methods: </strong>Computed tomography (CT) Scan Machine and Micro CT Scan Machine were used for this study. Gross anatomical studies were performed on prepared bones using the method of using insects.</p><p><strong>Results: </strong>The carpal region consists of 10 bones (5 proximal and 5 distal), whereas the tarsal region features a fused Astragalus and four distal bones, both following a 2.3.3.3.2 phalangeal formula. Significant sexual dimorphism is limited to the pelvic girdle, where males exhibit a more prominent pubic ridge and an ossified symphysis compared to the predominantly cartilaginous structure in females.</p><p><strong>Conclusions: </strong>The European pond turtle displays distinct anatomical characteristics compared to other turtle species, particularly aquatic ones. Notably, this turtle exhibits nails on all its manus digits, which are elongated and non-fin-like. In conclusion, the use of diagnostic imaging techniques, such as CT scans and micro-CT scans, in the study of turtle skeletons has proven to be highly beneficial. These techniques enable researchers to accurately determine the direction and position of bones, even in species with complex anatomical features like turtles.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71019"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13347142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148413038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference Intervals for Schirmer Tear Test and Intraocular Pressure in Healthy Hamdani Crossbred Sheep.","authors":"Kadir Sulu, Mustafa Baris Akgul, Ali Gulaydin, Maruf Yilmaz, Bahar Erden, Ali Osman Turgut","doi":"10.1002/vms3.71018","DOIUrl":"10.1002/vms3.71018","url":null,"abstract":"<p><strong>Background: </strong>Breed-specific reference intervals for the Schirmer tear test (STT) and intraocular pressure (IOP) in Hamdani crossbred sheep have not been established.</p><p><strong>Objectives: </strong>To determine reference intervals for STT and IOP in healthy Hamdani crossbred sheep.</p><p><strong>Methods: </strong>A total of 200 clinically healthy animals were stratified into four age groups: < 6 months, 6 months to 1 year, 1-2 years, and > 2 years. Each group comprised equal numbers of males (n = 25) and females (n = 25). Tear production was evaluated using STT-1, and IOP was measured with a rebound tonometer. Group-based mean values were calculated using representative values obtained by averaging measurements from both eyes.</p><p><strong>Results: </strong>Mean values and reference intervals for STT and IOP were established for each age group. No sex-related differences were observed in STT values. However, age-related differences were observed only in females, with higher STT values in hoggets than lambs (p < 0.01). For IOP, ewes had higher values than rams (p < 0.01). Age-related differences in IOP were observed only in males, with rams showing lower IOP values than suckling lambs and hoggets (p < 0.01).</p><p><strong>Conclusions: </strong>This study establishes reference intervals for STT and IOP in healthy Hamdani crossbred sheep and provides clinically relevant baseline data for ophthalmic assessment.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71018"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13584875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148158304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Mineral Water (Hora) Supplementation Improves Growth Performance and Carcass Characteristics of Sheep in Ethiopia.","authors":"Ashenafi Miresa Kenea, Taye Tolemariam, Belay Duguma, Ellen S Dierenfeld, Abebe Nigussie, Feyissa Begna, Sisay Bekele","doi":"10.1002/vms3.71008","DOIUrl":"10.1002/vms3.71008","url":null,"abstract":"<p><strong>Background: </strong>Natural mineral water (hora) is a mineral source widely available in Ethiopia. However, its specific impacts on sheep growth performance and carcass characteristics remain understudied.</p><p><strong>Objective: </strong>To evaluate the effect of hora supplementation on nutrient intake, growth performance and carcass characteristics of sheep.</p><p><strong>Methods: </strong>Twenty Horro breed sheep with an initial body weight (IBW) of 17.27 ± 0.71 kg were assigned to five treatment groups in a randomized complete block design (RCBD), blocked on the basis of their IBW. The treatments included control group (CON) fed basal diet only, and four treatment groups (H-200, H-300, H-400, and H-500) receiving 200, 300, 400, and 500 mL, of hora per day respectively. All sheep were fed grass hay and water ad libitum. Throughout the feeding trial data on dry matter (DM) and nutrient intake, body weight changes and various carcass traits were measured.</p><p><strong>Results: </strong>Hora supplementation significantly improved (p < 0.001) daily DM and nutrient intake. The H-400 group exhibited the highest intake, with total DM intake reaching 0.99 kg/day (p < 0.001), whereas CON and H-300 showed the lowest. Final body weight, live weight gain, average daily gain, and feed conversion efficiency were significantly higher (p < 0.001) in the H-400 group. Polynomial contrasts revealed significant quadratic patterns (p < 0.05) for most intake and growth performance parameters. A significant linear trend (p < 0.001) was observed for empty body weight, hot carcass weight, and rib-eye area. Dressing and saleable percentages remained unaffected by hora supplementation (p > 0.05). Total edible offal components showed a significant quadratic pattern (p = 0.001), whereas total non-edible offal showed a significant linear trend (p = 0.002).</p><p><strong>Conclusions: </strong>Supplementation with 400 mL/day of hora is the optimal dose for significantly improving sheep growth performance, nutrient intake, and carcass characteristics. Overall, although hora provides clear benefits, its effects are dose-dependent, with potential diminishing returns beyond the 400 mL/day level.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71008"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13240198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148043942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Knowledge, Attitudes and Practices Regarding Brucellosis Among High-Risk Occupational Groups.","authors":"Md Abdur Nur Sakib, Tajul Islam Mamun, Sumayae Binta Khaiat, Prity Ranjan Talukdar, Mahfuzur Rahman, Raisa Islam, Shabbir Ahmed, Fahmida Yesmin Oishee, Milton Roy, Md Mukter Hossain","doi":"10.1002/vms3.71038","DOIUrl":"10.1002/vms3.71038","url":null,"abstract":"<p><strong>Background: </strong>Brucellosis is a neglected but significant zoonotic disease that poses serious public health and economic challenges, particularly in low- and middle-income countries.</p><p><strong>Objectives: </strong>This study aimed to assess the knowledge, attitudes and practices (KAP) related to brucellosis among high-risk occupational groups in Bangladesh, including dairy workers, livestock handlers and butchers.</p><p><strong>Methods: </strong>A cross-sectional study was conducted through face-to-face interviews using a structured, closed-ended questionnaire. A total of 435 participants from five districts were randomly selected. The questionnaire covered socio-demographic characteristics, knowledge of brucellosis, attitudes towards the disease and preventive practices. Descriptive statistics, univariable and multivariable logistic regression and correlation analyses were performed.</p><p><strong>Results: </strong>Overall, 186 (43%) of participants demonstrated good knowledge about brucellosis. The majority of respondents (96%) exhibited a positive attitude towards the disease, and 59% reported correct practices at their workplaces. Notably, 52.9% of participants were aware of brucellosis transmission routes, and 90.6% reported proper disposal of aborted animal materials. Dairy workers were significantly more knowledgeable (adjusted odds ratio [AOR] = 2.00; 95% confidence interval [CI]: 1.11-3.63; p = 0.021) and demonstrated better practices (AOR = 4.69, CI: 2.30-9.88, p < 0.001) compared to butchers. Participants who had received training were significantly more likely to have good knowledge (AOR: 2.01; 95% CI: 1.12-3.61; p = 0.019) and to practice correct behaviours (AOR: 4.42; 95% CI: 2.11-8.83; p < 0.001) than those without training. A statistically significant, very weak positive correlation was observed between knowledge and practice scores.</p><p><strong>Conclusions: </strong>This study highlights considerable gaps in knowledge and preventive practices among high-risk occupational groups in Bangladesh. Targeted educational interventions and comprehensive control programmes are urgently needed, especially for livestock handlers, butchers and individuals with lower educational levels, to reduce the risk of animal-to-human transmission and control the spread of brucellosis in the country.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71038"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}