Sedrati Tahar, Touati Abdelaziz, Tennah Safia, Menoueri M Nabil, Seydina M Diene
{"title":"阿尔及利亚原料乳中分离的阴沟肠杆菌ST48株粘菌素耐药性检测及鉴定","authors":"Sedrati Tahar, Touati Abdelaziz, Tennah Safia, Menoueri M Nabil, Seydina M Diene","doi":"10.1002/vms3.70501","DOIUrl":null,"url":null,"abstract":"<p><p>An Enterobacter cloacae ST48 isolate exhibiting a colistin-heteroresistant phenotype was obtained from raw cow milk in Algeria. Two subpopulations were detected, both carrying the bla<sub>TEM-1</sub> gene. While one subpopulation (E. cloacae TS34-a) was highly susceptible to colistin (MIC 0.06 µg/mL), the other (E. cloacae TS34-b) was resistant (MIC 8 µg/mL). Population analysis profiling (PAP) confirmed heteroresistance. PCR amplification revealed the absence of mcr genes in both subpopulations. However, multiple non-synonymous mutations, including a stop-codon mutation in phoP gene, were found in the resistant subpopulation. This is the first report of colistin-heteroresistant E. cloacae isolated from raw milk, underscoring the importance of vigilance in the dairy industry and food safety.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 4","pages":"e70501"},"PeriodicalIF":1.7000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12244255/pdf/","citationCount":"0","resultStr":"{\"title\":\"Detection and Characterization of Colistin Heteroresistance in an Enterobacter cloacae ST48 Strain Isolated From Raw Milk in Algeria.\",\"authors\":\"Sedrati Tahar, Touati Abdelaziz, Tennah Safia, Menoueri M Nabil, Seydina M Diene\",\"doi\":\"10.1002/vms3.70501\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An Enterobacter cloacae ST48 isolate exhibiting a colistin-heteroresistant phenotype was obtained from raw cow milk in Algeria. Two subpopulations were detected, both carrying the bla<sub>TEM-1</sub> gene. While one subpopulation (E. cloacae TS34-a) was highly susceptible to colistin (MIC 0.06 µg/mL), the other (E. cloacae TS34-b) was resistant (MIC 8 µg/mL). Population analysis profiling (PAP) confirmed heteroresistance. PCR amplification revealed the absence of mcr genes in both subpopulations. However, multiple non-synonymous mutations, including a stop-codon mutation in phoP gene, were found in the resistant subpopulation. This is the first report of colistin-heteroresistant E. cloacae isolated from raw milk, underscoring the importance of vigilance in the dairy industry and food safety.</p>\",\"PeriodicalId\":23543,\"journal\":{\"name\":\"Veterinary Medicine and Science\",\"volume\":\"11 4\",\"pages\":\"e70501\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12244255/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Medicine and Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1002/vms3.70501\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medicine and Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/vms3.70501","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Detection and Characterization of Colistin Heteroresistance in an Enterobacter cloacae ST48 Strain Isolated From Raw Milk in Algeria.
An Enterobacter cloacae ST48 isolate exhibiting a colistin-heteroresistant phenotype was obtained from raw cow milk in Algeria. Two subpopulations were detected, both carrying the blaTEM-1 gene. While one subpopulation (E. cloacae TS34-a) was highly susceptible to colistin (MIC 0.06 µg/mL), the other (E. cloacae TS34-b) was resistant (MIC 8 µg/mL). Population analysis profiling (PAP) confirmed heteroresistance. PCR amplification revealed the absence of mcr genes in both subpopulations. However, multiple non-synonymous mutations, including a stop-codon mutation in phoP gene, were found in the resistant subpopulation. This is the first report of colistin-heteroresistant E. cloacae isolated from raw milk, underscoring the importance of vigilance in the dairy industry and food safety.
期刊介绍：
Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science.
Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.
We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell.
Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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