{"title":"澳大利亚纯种马比赛后血浆中硫醇氧化白蛋白水平升高。","authors":"Christopher James, Erin M Lloyd, Peter G Arthur","doi":"10.1002/vms3.70487","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The extent to which muscle strain or tears are affecting race performance in horses is unknown because it is difficult to objectively identify muscle damage. One approach includes the use of physiological markers in blood. Recently, we linked the level of plasma thiol-oxidised albumin, an oxidative stress biomarker, to muscle damage in humans.</p><p><strong>Objectives: </strong>This aim of this work was to investigate whether the level of plasma thiol-oxidised albumin was elevated in the days following a thoroughbred horse race.</p><p><strong>Study design: </strong>'In vivo' experiments.</p><p><strong>Methods: </strong>Blood collection was performed by each trainer at their respective stable. Dried blood samples were collected for the level of thiol-oxidised albumin before and each day for 7 days post-race. Liquid blood samples were collected for analysis of the muscle enzymes creatine kinase (CK) and aspartate amino transferase (AST) before and on Day 2 and 5 post-race and were analysed by external pathology centre.</p><p><strong>Results: </strong>The level of thiol-oxidised albumin peaked at 2 days post-race, increasing by 3.9 ± 0.7% (p < 0.0001) and returned to pre-race levels by Day 5. Thiol-oxidised albumin also correlated with the activity of AST (R<sup>2</sup> = 0.2, p = 0.01). The time of recovery for thiol-oxidised albumin varied between individual horses, some recovered at Day 3 post-race whereas others extended beyond 7 days post-race.</p><p><strong>Main limitations: </strong>No histological or imaging diagnosis was conducted to confirm that horses with elevated levels of thiol-oxidised albumin also had definitive evidence of muscle damage. The activity of CK and AST was not measured daily, as was the case for the level of thiol-oxidised albumin.</p><p><strong>Conclusions: </strong>The level of thiol-oxidised albumin has the potential to be useful in managing recovery and return to training or competition in horse following a bout of damaging exercise, particularly given the ease of collecting small serial small blood samples.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"11 4","pages":"e70487"},"PeriodicalIF":1.7000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12249229/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Level of Thiol-Oxidised Plasma Albumin Is Elevated Following a Race in Australian Thoroughbred Horses.\",\"authors\":\"Christopher James, Erin M Lloyd, Peter G Arthur\",\"doi\":\"10.1002/vms3.70487\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The extent to which muscle strain or tears are affecting race performance in horses is unknown because it is difficult to objectively identify muscle damage. One approach includes the use of physiological markers in blood. Recently, we linked the level of plasma thiol-oxidised albumin, an oxidative stress biomarker, to muscle damage in humans.</p><p><strong>Objectives: </strong>This aim of this work was to investigate whether the level of plasma thiol-oxidised albumin was elevated in the days following a thoroughbred horse race.</p><p><strong>Study design: </strong>'In vivo' experiments.</p><p><strong>Methods: </strong>Blood collection was performed by each trainer at their respective stable. Dried blood samples were collected for the level of thiol-oxidised albumin before and each day for 7 days post-race. Liquid blood samples were collected for analysis of the muscle enzymes creatine kinase (CK) and aspartate amino transferase (AST) before and on Day 2 and 5 post-race and were analysed by external pathology centre.</p><p><strong>Results: </strong>The level of thiol-oxidised albumin peaked at 2 days post-race, increasing by 3.9 ± 0.7% (p < 0.0001) and returned to pre-race levels by Day 5. Thiol-oxidised albumin also correlated with the activity of AST (R<sup>2</sup> = 0.2, p = 0.01). The time of recovery for thiol-oxidised albumin varied between individual horses, some recovered at Day 3 post-race whereas others extended beyond 7 days post-race.</p><p><strong>Main limitations: </strong>No histological or imaging diagnosis was conducted to confirm that horses with elevated levels of thiol-oxidised albumin also had definitive evidence of muscle damage. The activity of CK and AST was not measured daily, as was the case for the level of thiol-oxidised albumin.</p><p><strong>Conclusions: </strong>The level of thiol-oxidised albumin has the potential to be useful in managing recovery and return to training or competition in horse following a bout of damaging exercise, particularly given the ease of collecting small serial small blood samples.</p>\",\"PeriodicalId\":23543,\"journal\":{\"name\":\"Veterinary Medicine and Science\",\"volume\":\"11 4\",\"pages\":\"e70487\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12249229/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Medicine and Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1002/vms3.70487\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medicine and Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/vms3.70487","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
背景:肌肉拉伤或撕裂在多大程度上影响马的比赛表现是未知的,因为很难客观地识别肌肉损伤。一种方法包括使用血液中的生理标记物。最近,我们将血浆硫醇氧化白蛋白(一种氧化应激生物标志物)的水平与人类肌肉损伤联系起来。目的:这项工作的目的是研究血浆硫醇氧化白蛋白水平是否在纯种马比赛后的几天内升高。研究设计:“体内”实验。方法:各训练师在各自的马厩进行采血。在赛前和赛后7天每天采集干血样本检测巯基氧化白蛋白水平。于赛前、赛后第2天、第5天采集血液,检测肌酶肌酸激酶(CK)和天冬氨酸氨基转移酶(AST),并送交外部病理中心检测。结果:巯基氧化白蛋白水平在赛后2 d达到峰值,升高3.9±0.7% (p 2 = 0.2, p = 0.01)。巯基氧化白蛋白的恢复时间因马而异,有些马在赛后第3天恢复,而另一些马则在赛后7天以上恢复。主要局限性:没有进行组织学或影像学诊断来证实具有高水平硫醇氧化白蛋白的马也有明确的肌肉损伤证据。不测量CK和AST的活性,也不测量硫醇氧化白蛋白的水平。结论:硫醇氧化白蛋白的水平有可能对马在一次破坏性运动后的恢复和恢复训练或比赛中有用,特别是考虑到收集小系列小血液样本的便性。
The Level of Thiol-Oxidised Plasma Albumin Is Elevated Following a Race in Australian Thoroughbred Horses.
Background: The extent to which muscle strain or tears are affecting race performance in horses is unknown because it is difficult to objectively identify muscle damage. One approach includes the use of physiological markers in blood. Recently, we linked the level of plasma thiol-oxidised albumin, an oxidative stress biomarker, to muscle damage in humans.
Objectives: This aim of this work was to investigate whether the level of plasma thiol-oxidised albumin was elevated in the days following a thoroughbred horse race.
Study design: 'In vivo' experiments.
Methods: Blood collection was performed by each trainer at their respective stable. Dried blood samples were collected for the level of thiol-oxidised albumin before and each day for 7 days post-race. Liquid blood samples were collected for analysis of the muscle enzymes creatine kinase (CK) and aspartate amino transferase (AST) before and on Day 2 and 5 post-race and were analysed by external pathology centre.
Results: The level of thiol-oxidised albumin peaked at 2 days post-race, increasing by 3.9 ± 0.7% (p < 0.0001) and returned to pre-race levels by Day 5. Thiol-oxidised albumin also correlated with the activity of AST (R2 = 0.2, p = 0.01). The time of recovery for thiol-oxidised albumin varied between individual horses, some recovered at Day 3 post-race whereas others extended beyond 7 days post-race.
Main limitations: No histological or imaging diagnosis was conducted to confirm that horses with elevated levels of thiol-oxidised albumin also had definitive evidence of muscle damage. The activity of CK and AST was not measured daily, as was the case for the level of thiol-oxidised albumin.
Conclusions: The level of thiol-oxidised albumin has the potential to be useful in managing recovery and return to training or competition in horse following a bout of damaging exercise, particularly given the ease of collecting small serial small blood samples.
期刊介绍:
Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science.
Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.
We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell.
Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.