A Hydrogel Scaffold Incorporating Fennel Seed Extract Induces Osteogenic Differentiation in Mesenchymal Stem Cells.

IF 1.7 3区 农林科学 Q2 VETERINARY SCIENCES
Kosar Heidari, Tayebeh Mohammadi, Leila Soltani, Mehrdad PooyanMehr
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引用次数: 0

Abstract

Background: Tissue engineering utilizing hydrogels and scaffolds composed of organic and synthetic materials has emerged as a promising approach for bone repair. Chitosan, a biocompatible natural polymer, supports bone healing and possesses antibacterial and antioxidant properties. Fennel (Foeniculum vulgare), known for its traditional use in treating osteoporosis in females, exhibits phytoestrogenic properties.

Objectives: This study investigated the osteogenic differentiation of mesenchymal stem cells (MSCs) cultured within chitosan hydrogels incorporating fennel seed extract.

Methods: Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) were employed to characterize the chitosan films containing fennel extract. MSCs were isolated from ovine foetal bone marrow and subjected to various concentrations of fennel hydroalcoholic extract and hydrogels containing these extracts. Cell viability was assessed using the MTT assay. Following osteoblast differentiation, gene expression of osteogenic markers (ALP, Runx2 and COL1A1) was evaluated using real-time PCR, alongside assessments of calcium deposition, alkaline phosphatase activity and ALP expression. Alizarin red staining was performed to quantify mineralized matrix deposition.

Results: The MTT assay revealed that the 0.5% extract treatment group significantly enhanced MSC proliferation. Notably, MSCs cultured on the chitosan film containing 1% fennel extract demonstrated increased numbers of hydroxyapatite deposits, elevated ALP activity and calcium content and upregulated expression of osteogenic genes (ALP, Runx2 and COL1A1). Acridine orange/ethidium bromide staining confirmed the non-toxicity of the extract in all treatment groups.

Conclusion: These findings demonstrate that chitosan hydrogels incorporating fennel seed extract can effectively induce osteogenic differentiation of MSCs, highlighting their potential as a promising strategy for bone tissue regeneration.

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含茴香籽提取物的水凝胶支架诱导间充质干细胞成骨分化。
背景:利用有机材料和合成材料组成的水凝胶和支架的组织工程已经成为一种很有前途的骨修复方法。壳聚糖是一种生物相容性天然聚合物,支持骨愈合,具有抗菌和抗氧化特性。茴香(Foeniculum vulgare),以其治疗女性骨质疏松症的传统用途而闻名,具有植物雌激素特性。目的:研究茴香籽提取物壳聚糖水凝胶对间充质干细胞成骨分化的影响。方法:采用傅里叶变换红外光谱(FTIR)和扫描电镜(SEM)对茴香提取物壳聚糖膜进行表征。从绵羊胎儿骨髓中分离出间充质干细胞,并将其置于不同浓度的茴香水酒精提取物和含有这些提取物的水凝胶中。采用MTT法测定细胞活力。成骨细胞分化后,采用实时荧光定量PCR技术评估成骨标志物(ALP、Runx2和COL1A1)的基因表达,同时评估钙沉积、碱性磷酸酶活性和ALP表达。茜素红染色定量矿化基质沉积。结果:MTT实验显示,0.5%提取物处理组明显促进MSC增殖。值得注意的是,在含有1%茴香提取物的壳聚糖膜上培养的间充质干细胞显示出羟基磷灰石沉积物数量增加,ALP活性和钙含量升高,成骨基因(ALP, Runx2和COL1A1)表达上调。吖啶橙/溴化乙啶染色证实提取物在所有治疗组中均无毒性。结论:茴香籽提取物壳聚糖水凝胶可有效诱导骨髓间充质干细胞成骨分化,是一种有前景的骨组织再生策略。
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来源期刊
Veterinary Medicine and Science
Veterinary Medicine and Science Veterinary-General Veterinary
CiteScore
3.00
自引率
0.00%
发文量
296
期刊介绍: Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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