Trends in biotechnology最新文献_第5页

Translational benchmarking of 3D in vitro models of traumatic brain injury. 外伤性脑损伤三维体外模型的翻译标杆研究。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-22 DOI: 10.1016/j.tibtech.2026.09.014

Peter Hsi, Michael L Lovett, Vishal Tandon, David L Kaplan

引用次数: 0

Asking pragmatic and RAD questions about organoid intelligence. 问一些关于类器官智能的实用的RAD问题。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-22 DOI: 10.1016/j.tibtech.2026.09.004

Andrew J Barnhart

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Why cell agriculture struggles with meat and opportunities for non-food tissues. 为什么细胞农业与肉类和非食物组织的机会斗争。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-17 DOI: 10.1016/j.tibtech.2026.09.001

Soon Mo Choi, Sun Mi Zo, Ankur Sood, Do Hyun Lee, Sung Soo Han

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Liquid biopsy in glioblastoma: emerging technologies and translational opportunities. 胶质母细胞瘤液体活检：新兴技术和转化机会。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-16 DOI: 10.1016/j.tibtech.2026.08.007

Olawumi Giwa, Yonit Leykind, Itamar Altman, Tali Siegal, Milana Frenkel-Morgenstern

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Cross-target and cell-preferential CRISPR-Cas9 inhibition with carbohydrate-tagged oligonucleotides. 碳水化合物标记的寡核苷酸对CRISPR-Cas9的交叉靶标和细胞优先抑制。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-15 DOI: 10.1016/j.tibtech.2026.08.014

Bingzhi Li, Shulin Wang, Min Cao, Shuyao Ma, Xin Wang, Guang Yang, Jing Wu, Xing Zhang

{"title":"Cross-target and cell-preferential CRISPR-Cas9 inhibition with carbohydrate-tagged oligonucleotides.","authors":"Bingzhi Li, Shulin Wang, Min Cao, Shuyao Ma, Xin Wang, Guang Yang, Jing Wu, Xing Zhang","doi":"10.1016/j.tibtech.2026.08.014","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.014","url":null,"abstract":"Precise control of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) activity is important for limiting off-target effects and chromosomal rearrangements. Existing inhibitors, including anti-CRISPR proteins and spacer-targeting oligonucleotides, can be constrained by immunogenicity, target-sequence dependence, or delivery challenges. Here, we developed single-stranded DNA oligonucleotides that target conserved regions of the Streptococcus pyogenes Cas9 single-guide RNA (sgRNA) scaffold rather than the variable spacer. Screening identified single-stranded DNA 7 (ssDNA7), which targets stem-loop-1 and the adjacent linker and inhibits Cas9 activity across multiple tested spacer sequences and genomic loci without redesigning the inhibitor. Carbohydrate conjugation improved oligonucleotide stability and preferentially enhanced inhibitory activity in selected liver-derived or cancer cell models. Amplicon sequencing confirmed inhibition of endogenous editing in multiple cell models and normal liver-derived organoids, and supported cell-preferential inhibition in matched cell comparisons. These findings establish sgRNA-scaffold targeting as a strategy for cross-target inhibition of Cas9 and show that carbohydrate conjugation can tune its cellular activity.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148978105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme evolution and metabolic engineering for 5-aminolevulinic acid production. 5-氨基乙酰丙酸生产的酶进化和代谢工程。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-15 DOI: 10.1016/j.tibtech.2026.08.015

Sefli Sri Wahyu Effendi, Yu-Chieh Lin, I-Son Ng

引用次数: 0

DipTRANS: an improved method for in planta transformation and genome engineering in Nicotiana benthamiana. DipTRANS：一种改良的烟草植物转化和基因组工程方法。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-12 DOI: 10.1016/j.tibtech.2026.08.013

Fazhan Qi, Weiwei Chen, Jon P Cody, Hao Cheng, Jiayi Gao, James C Chamness, Yumeng Jiang, Fang Wei, Daniel F Voytas, Gongyao Shi

{"title":"DipTRANS: an improved method for in planta transformation and genome engineering in Nicotiana benthamiana.","authors":"Fazhan Qi, Weiwei Chen, Jon P Cody, Hao Cheng, Jiayi Gao, James C Chamness, Yumeng Jiang, Fang Wei, Daniel F Voytas, Gongyao Shi","doi":"10.1016/j.tibtech.2026.08.013","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.013","url":null,"abstract":"Plant transformation remains constrained by labor-intensive tissue culture. Our previous work showed that direct delivery of developmental regulators (DRs) can induce de novo meristems on plants, offering a promising transformation approach. In this resource article, we introduced DipTRANS (Direct in planta Transformation), an optimized, soil-based heritable transformation platform for Nicotiana benthamiana that bypasses sterile culture entirely. DipTRANS is built on DR-induced de novo meristem formation. After optimizing parameters, including regulator combinations, Agrobacterium strain, and infiltration density, DipTRANS yielded transformation efficiencies to 46.7%. Developmental abnormalities associated with regulator expression are resolved through cutting-based propagation and virus-induced transgene excision, enabling recovery of fertile, transgenic progeny. Furthermore, DipTRANS supports tissue culture-free, transgene-free iterative genome modification via virus-induced genome editing. Overall, DipTRANS enables the generation of transgenic plants within 30 days and engineered progeny within 90 days. This methodology provides a rapid, versatile platform and a blueprint for extending direct in planta transformation to other plant species.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148956897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A combinatorial construct library enables an expanded expression range of secreted therapeutic proteins by probiotic yeast. 组合构建库使益生菌酵母菌分泌的治疗性蛋白的表达范围扩大。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-11 DOI: 10.1016/j.tibtech.2026.08.012

Miranda J Wallace, Christina Kratschmer, Brandon Brazil, Ilham Matiker, Esse M Evbuomwan, Jerome Prusa, Jasmine Najari, Rehan Mehta, Ana Romani, Sophia Zhang, Jie Ning, Ryan A Hagenson, James Liao, Bin Wang, Beth A Helmink, Gautam Dantas

{"title":"A combinatorial construct library enables an expanded expression range of secreted therapeutic proteins by probiotic yeast.","authors":"Miranda J Wallace, Christina Kratschmer, Brandon Brazil, Ilham Matiker, Esse M Evbuomwan, Jerome Prusa, Jasmine Najari, Rehan Mehta, Ana Romani, Sophia Zhang, Jie Ning, Ryan A Hagenson, James Liao, Bin Wang, Beth A Helmink, Gautam Dantas","doi":"10.1016/j.tibtech.2026.08.012","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.012","url":null,"abstract":"Orally administered engineered probiotics, including Saccharomyces cerevisiae var. boulardii (Sb), are of emerging interest as protein therapeutic delivery platforms to treat gastrointestinal diseases. Tools to readily optimize protein output are required to optimize the therapeutic index of Sb-produced therapies. In this study, a 125-plex Sb secretion construct library was developed consisting of all possible combinations of five promoters, five secretion signals, and five terminators, which enabled a greater than 1800-fold range in Sb expression of a Gaussia luciferase (GLuc) reporter. Secretion signal and promoter identities had significant effects on secretion output. This library further enabled a 28-fold improvement of binding activity of Sb-secreted haPD-1, an established anti-tumor immunotherapeutic, and improved haPD-1 detection in mouse stool samples following oral gavage of Sb_haPD-1. Sb secretion trends of both GLuc and haPD-1 in vitro mirrored payload expression in vivo. This protein secretion library toolkit will serve as a valuable resource to rapidly optimize protein therapeutic output from engineered Sb.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148945789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering biosensors to enhance monoterpene indole alkaloid production in yeast. 工程生物传感器提高单萜吲哚生物碱在酵母中的生产。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-09 DOI: 10.1016/j.tibtech.2026.08.011

Maxence Holtz, Simon D'Oelsnitz, Cecília Castellví Domingo, Niklas G Madsen, Mars Yu Hong, Jonathan Asmund Arnesen, Aafke C A van Aalst, Samantha de Haan, Christopher K Weingarten, Ditte H Welner, Pamela A Silver, Yan Jessie Zhang, Michael K Jensen, Carlos G Acevedo-Rocha

{"title":"Engineering biosensors to enhance monoterpene indole alkaloid production in yeast.","authors":"Maxence Holtz, Simon D'Oelsnitz, Cecília Castellví Domingo, Niklas G Madsen, Mars Yu Hong, Jonathan Asmund Arnesen, Aafke C A van Aalst, Samantha de Haan, Christopher K Weingarten, Ditte H Welner, Pamela A Silver, Yan Jessie Zhang, Michael K Jensen, Carlos G Acevedo-Rocha","doi":"10.1016/j.tibtech.2026.08.011","DOIUrl":"10.1016/j.tibtech.2026.08.011","url":null,"abstract":"Monoterpene indole alkaloids (MIAs) are medicinally valuable plant natural products, but their scarcity and structural complexity hinder conventional production. Microbial cell factories offer a sustainable alternative, although optimization of their long biosynthetic pathways is constrained by costly, low-throughput analytics. In this article, we evolved the promiscuous bacterial transcription factor RamR to detect five MIAs, obtaining sensitive and selective biosensor variants with EC50 values below 10 μM. We functionalized RamR in yeast and used it to screen a 188-gene overexpression library for improved strictosidine (STR) production, the common precursor for all MIAs. Biosensor fluorescence strongly correlated with HPLC measurements (R2 = 0.932), enabling identification of engineering targets that, when combined, increased STR titers more than threefold to over 220 mg/l. This RamR-based biosensing platform enables cost-effective screening and makes large biological design spaces experimentally accessible, accelerating strain and enzyme engineering for MIAs and other complex natural products.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrified future with microbial electrochemical technologies: how to realize the dream? 微生物电化学技术的电气化未来：如何实现梦想？

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-09 DOI: 10.1016/j.tibtech.2026.07.031

Sanne M de Smit, Largus Lars T Angenent, Federico Aulenta, Abraham Esteve-Nunez, Albert Guisasola, Falk Harnisch, Elizabeth S Heidrich, Ludovic Jourdin, Marika Kokko, Rikke Linssen, Deepak Pant, Catarina M Paquete, Jo Philips, Sebastià Puig, Korneel Rabaey, Miriam A Rosenbaum, Uwe Schröder, Tom Sleutels, César I Torres, Marianna Villano, Annemiek Ter Heijne

{"title":"An electrified future with microbial electrochemical technologies: how to realize the dream?","authors":"Sanne M de Smit, Largus Lars T Angenent, Federico Aulenta, Abraham Esteve-Nunez, Albert Guisasola, Falk Harnisch, Elizabeth S Heidrich, Ludovic Jourdin, Marika Kokko, Rikke Linssen, Deepak Pant, Catarina M Paquete, Jo Philips, Sebastià Puig, Korneel Rabaey, Miriam A Rosenbaum, Uwe Schröder, Tom Sleutels, César I Torres, Marianna Villano, Annemiek Ter Heijne","doi":"10.1016/j.tibtech.2026.07.031","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.07.031","url":null,"abstract":"Microbial electrochemical technologies (METs) are emerging with innovative applications in various fields by harnessing the combined action of electrodes and microbes. What if METs could be the key to circularity in wastewater treatment? What if METs could be employed to convert electricity and CO2, or waste, into valuable organics? What if new bioinspired materials could be produced through METs? What if METs could be used as smart bio-based sensors? We provide our vision for the future of METs in four sectors: water treatment, industrial biotechnology, electronics, and biosensors. We showcase why and how METs could advance or replace state-of-the-art technologies and discuss the challenges that must be overcome to make METs part of an electrified future powered by microbes.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0