Trends in biotechnology最新文献_第5页

Microbial tools and regulatory innovations for climate-resilient winemaking. 气候适应型葡萄酒酿造的微生物工具和监管创新。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-18 DOI: 10.1016/j.tibtech.2025.09.001

Javier Vicente, Santiago Benito, Domingo Marquina, Antonio Santos

引用次数: 0

Nanobotic microbiomes against emerging vascular pathogens in agriculture. 纳米微生物组在农业中抗新出现的血管病原体。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-18 DOI: 10.1016/j.tibtech.2025.08.015

Ayesha Ahmed, Yongmei Li, Yueqiu He, Dake Zhao, Shahzad Munir, Rajender S Varma

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Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering. 序列协同进化引导异戊二烯合成酶工程的半自动化生物铸造工作流程。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-13 DOI: 10.1016/j.tibtech.2025.08.007

Georgii Emelianov, Dong-Uk Song, Aporva Kamath, Hyeongseop Kim, Geunyeong Lee, Ha-Neul Kim, Kil Koang Kwon, Bong Hyun Sung, Dae-Hee Lee, Nathan J Hillson, Haseong Kim, Sanguk Kim, Hyewon Lee, Seung-Goo Lee

{"title":"Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering.","authors":"Georgii Emelianov, Dong-Uk Song, Aporva Kamath, Hyeongseop Kim, Geunyeong Lee, Ha-Neul Kim, Kil Koang Kwon, Bong Hyun Sung, Dae-Hee Lee, Nathan J Hillson, Haseong Kim, Sanguk Kim, Hyewon Lee, Seung-Goo Lee","doi":"10.1016/j.tibtech.2025.08.007","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.08.007","url":null,"abstract":"Biofoundries serve as transformative platforms for accelerating the engineering of enzymes and microorganisms toward biomanufacturing. In this study, we developed scalable enzyme engineering workflows tailored for biofoundry applications, focusing on isoprene synthase (IspS) - a critical rate-limiting enzyme in the isoprene biosynthesis. By integrating computational mutation design based on sequence coevolution analysis and laboratory automation, we conducted three rounds of site-directed mutagenesis and screening. Approximately 100 genetic mutants were synthesized per round and these workflows can be easily scaled up to thousands without extensive optimization. Moreover, this approach enabled the rapid identification of IspS variants with up to 4.5-fold improvement in catalytic efficiency and simultaneously enhanced thermostability. Additionally, introducing the engineered IspS into Methylococcus capsulatus Bath improved methane-to-isoprene bioconversion, achieving a titer of 319.6 mg/l. These scalable workflows establish a robust framework for enzyme engineering within biofoundries. This provides a basis for the development of innovative biotechnological advancements.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo continuous evolution via phenotypic sorting to alleviate metabolic bottlenecks in β-alanine production. 通过表型分选在体内持续进化以缓解β-丙氨酸生产中的代谢瓶颈。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-11 DOI: 10.1016/j.tibtech.2025.08.008

Fuqiang Song, Heng Zhang, Ke Wang, Kun Qiu, Shike Liu, Xinran Yin, Changtai Zhang, Jingwen Zhou

{"title":"In vivo continuous evolution via phenotypic sorting to alleviate metabolic bottlenecks in β-alanine production.","authors":"Fuqiang Song, Heng Zhang, Ke Wang, Kun Qiu, Shike Liu, Xinran Yin, Changtai Zhang, Jingwen Zhou","doi":"10.1016/j.tibtech.2025.08.008","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.08.008","url":null,"abstract":"β-Alanine is an important platform chemical whose biosynthesis efficiency is limited by pathway bottlenecks and enzyme constraints. In this study, to overcome genetic interactions for β-alanine production, we systematically engineered Escherichia coli MG1655 through modular pathway optimization and combinatorial regulation. To overcome the limitations of L-aspartate-α-decarboxylase from Bacillus subtilis (PanDbsu), we developed an in vivo evolution platform combining base-editing systems with biosensor guidance, thus generating PanDbsu variants with enhanced activity. This system facilitated high-throughput screening and real-time monitoring of β-alanine production and accelerated mutant selection. Furthermore, site saturation and iterative mutations identified a beneficial PanDbsuT4E mutant, which enhanced specific β-alanine production in engineered strain MA31 by 62.45%. Structural and functional analysis revealed that PanDbsuT4E stabilized its quaternary structure via a Glu-Lys salt bridge. This work describes a scalable strategy for addressing pathway bottlenecks and highlighted the potential of integrating protein engineering with biosensor-guided evolution to optimize microbial cell factories.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable protection of multiple hosts against polyphagous pests using Plant Probiotic-Based Gene Silencing. 利用植物益生菌基因沉默保护多宿主免受多食性害虫侵害。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-10 DOI: 10.1016/j.tibtech.2025.08.004

Jing Chen, Rong Zhang, Xun Zhang, Chengjin Li, Jie Yang, Xiangbo Kong, Fu Liu, Jiaxing Fang, Sufang Zhang

{"title":"Sustainable protection of multiple hosts against polyphagous pests using Plant Probiotic-Based Gene Silencing.","authors":"Jing Chen, Rong Zhang, Xun Zhang, Chengjin Li, Jie Yang, Xiangbo Kong, Fu Liu, Jiaxing Fang, Sufang Zhang","doi":"10.1016/j.tibtech.2025.08.004","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.08.004","url":null,"abstract":"RNAi is a gene-silencing mechanism mediated by double-stranded RNA (dsRNA) molecules and is a promising pest control technology. However, challenges, such as limited persistence or narrow plant protection spectra in current RNAi application strategies, hinder its effectiveness against polyphagous pests. Here, we report an approach using plant probiotics to express dsRNA, propelling sustainable protection of multiple host plants. A Bacillus strain isolated from host plants of the polyphagous pest Hyphantria cunea, was engineered to express dsRNA targeting the pest. The modified SH-F8 strain significantly reduced H. cunea pupation rates and increased mortality by disrupting both energy metabolism and cuticle formation. The engineered SH-F8 strain exhibited successful colonization in two host plant species of H. cunea under field conditions, with enhanced population densities appearing under high-temperature/high-humidity conditions. This approach, termed 'Plant Probiotic-Based Gene Silencing' (PPGS), may offer a sustainable solution for multi-plant protection against polyphagous pests.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethical and legal considerations of digital animal models: pioneering reduction and replacement. 数字动物模型的伦理和法律考虑：开创性的减少和替代。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-10 DOI: 10.1016/j.tibtech.2025.08.011

Federico Germani, Julian W März, Alessandro Luciani, Olivier Devuyst, Nikola Biller-Andorno

引用次数: 0

Food production from air: gas precision fermentation with hydrogen-oxidising bacteria. 从空气中生产食品：利用氧化氢细菌进行气体精密发酵。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-09 DOI: 10.1016/j.tibtech.2025.08.003

Margarita Bernal-Cabas, Karan Kumar, Owen Terpstra, Samira van den Bogaard, Aziz B Ammar, Susanna Mäkinen, Lukas Herwig, Marta de Almeida, Petri Tervasmäki, Lars M Blank, Tobias B Alter, Sonja Billerbeck

引用次数: 0

Metabolic and immunomodulatory control of type 2 diabetes via generating cellular itaconate reservoirs by inflammatory-targeting gene-therapy nanovesicles. 通过炎症靶向基因治疗纳米囊泡产生细胞衣康酸储库来控制2型糖尿病的代谢和免疫调节。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-08 DOI: 10.1016/j.tibtech.2025.07.025

An Lao, Weiqi Li, Yiting Sun, Yuting Cao, Yu Zhuang, Jianyong Wu, Dejian Li, Kaili Lin, Jing Mao, Jiaqiang Liu

{"title":"Metabolic and immunomodulatory control of type 2 diabetes via generating cellular itaconate reservoirs by inflammatory-targeting gene-therapy nanovesicles.","authors":"An Lao, Weiqi Li, Yiting Sun, Yuting Cao, Yu Zhuang, Jianyong Wu, Dejian Li, Kaili Lin, Jing Mao, Jiaqiang Liu","doi":"10.1016/j.tibtech.2025.07.025","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.07.025","url":null,"abstract":"Type 2 diabetes (T2D) is characterized by persistent and unresolved tissue inflammation caused by the infiltration and dysregulation of immune cells. Current therapeutics targeting inflammatory immune cells for T2D remain limited. In this study, we analyzed single cell RNA from metabolic organs in T2D, revealing increased macrophage accumulation and a pathogenic macrophage subpopulation defined as NOD-like receptor (NLR) family pyrin domain-containing 3 (NLRP3)+ inflammatory and metabolically activated macrophages. To target these inflammatory cells, we developed nanovesicles encapsulating mitochondrial metabolic enzyme-related gene segments [immune-responsive gene 1 (IRG1)-overexpression plasmids] with cell membrane decoration. The nanovesicles functioned as cellular itaconate producers that elegantly circumvented the drug utilization barriers of a classic NLRP3 inhibitor and, as a mitochondria-reprograming system, mitigated fatty acid (FA)-associated metabolic dysfunction. The nanovesicles reversed inflammation, restored metabolic functions, and ameliorated obesity. Therefore, the metabolic and immunomodulatory functions of nanovesicles may offer translational opportunities for the prevention and treatment of T2D.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flying seed-inspired sensors for remote environmental monitoring on Earth and beyond. 以种子为灵感的飞行传感器，用于地球内外的远程环境监测。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-06 DOI: 10.1016/j.tibtech.2025.08.005

Sagar Arya, Lukáš Spíchal, Radek Zbořil

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Engineering chimeric signaling proteins for microbial whole-cell biosensors: from design to deployment. 用于微生物全细胞生物传感器的工程嵌合信号蛋白：从设计到部署。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-02 DOI: 10.1016/j.tibtech.2025.08.002

Güniz Özer Bergman, Sonia Mecacci, Vitor A P Martins Dos Santos, Enrique Asin-Garcia

引用次数: 0