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Single cell technologies for monitoring protein secretion heterogeneity. 监测蛋白质分泌异质性的单细胞技术。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-12 DOI: 10.1016/j.tibtech.2024.02.011
Fabian Stefan Franz Hartmann, Mélanie Grégoire, Francesco Renzi, Frank Delvigne
{"title":"Single cell technologies for monitoring protein secretion heterogeneity.","authors":"Fabian Stefan Franz Hartmann, Mélanie Grégoire, Francesco Renzi, Frank Delvigne","doi":"10.1016/j.tibtech.2024.02.011","DOIUrl":"10.1016/j.tibtech.2024.02.011","url":null,"abstract":"<p><p>Cell-to-cell heterogeneity presents challenges across various fields, from biomedicine to bioproduction, where precise cellular responses are vital. While single cell technologies have significantly enhanced our understanding of population heterogeneity, the predominant focus has been on monitoring intracellular compounds. Recognizing the added complexity introduced by the secretion system, in this review, we first provide a systematic overview of the distinct steps necessary for driving protein secretion. We discuss the various sources of noise acting from the synthesized preprotein to the secretory protein released based on a Gram-positive cellular system as a model. We next explore the applicability of single cell technologies for monitoring protein secretion throughout these functional stages. We also emphasize the importance of applying these single cell technologies for monitoring protein secretion during bioproduction.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140120744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perfusion fermentation sets a path to democratize biomanufacturing. 灌注发酵为生物制造民主化开辟了道路。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-30 DOI: 10.1016/j.tibtech.2024.08.003
Kerry R Love, Stacy E Martin, Devin G Morrison, Laura E Crowell
{"title":"Perfusion fermentation sets a path to democratize biomanufacturing.","authors":"Kerry R Love, Stacy E Martin, Devin G Morrison, Laura E Crowell","doi":"10.1016/j.tibtech.2024.08.003","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.08.003","url":null,"abstract":"","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent progress in one-pot enzymatic synthesis and regeneration of high-value cofactors. 一步酶法合成和再生高价值辅助因子的最新进展。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-29 DOI: 10.1016/j.tibtech.2024.08.006
I-Son Ng, Po-Hsiang Wang, Wan-Wen Ting, Jiun-Jang Juo
{"title":"Recent progress in one-pot enzymatic synthesis and regeneration of high-value cofactors.","authors":"I-Son Ng, Po-Hsiang Wang, Wan-Wen Ting, Jiun-Jang Juo","doi":"10.1016/j.tibtech.2024.08.006","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.08.006","url":null,"abstract":"<p><p>One-pot enzymatic synthesis is flourishing in synthetic chemistry, heralding a sustainable and green era. Recent advancements enable the creation of complex enzymatic prosthetic groups and regeneration of enzymatic cofactors such as S-adenosylmethionine. The next frontier is to develop the effective and innovative cofactors for essential micronutrients, metabolic modulators, and biomedicines.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cyanobacterial biofilms: from natural systems to applications. 蓝藻生物膜：从自然系统到应用。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-29 DOI: 10.1016/j.tibtech.2024.08.005
Mahir Bozan, Hannah Berreth, Pia Lindberg, Katja Bühler
{"title":"Cyanobacterial biofilms: from natural systems to applications.","authors":"Mahir Bozan, Hannah Berreth, Pia Lindberg, Katja Bühler","doi":"10.1016/j.tibtech.2024.08.005","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.08.005","url":null,"abstract":"<p><p>Cyanobacteria are the ancestors of oxygenic photosynthesis. Fueled by light and water, their ability to reduce CO<sub>2</sub> to sugar holds potential for carbon-neutral production processes. Due to challenges connected to cultivation and engineering issues, cyanobiotechnology has yet to be able to establish itself broadly in industry. In recent years, applying cyanobacterial biofilms as whole-cell biocatalysts instead of suspension cultures has emerged as a novel concept to counteract low cell densities and low reaction stability, critical challenges in cyanobacterial applications. This review explores the potential of cyanobacterial biofilms for biotechnology and bioremediation. It briefly introduces cyanobacteria as primary producers in natural structured microbial communities; describes various applications in biotechnology and bioremediation; and discusses innovations, challenges, and future trends in this exciting research field.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unlocking the potential of cyanobacteria: a high-throughput strategy for enhancing biocatalytic performance through genetic optimization. 释放蓝藻的潜力：通过基因优化提高生物催化性能的高通量策略。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-29 DOI: 10.1016/j.tibtech.2024.07.011
Julia Jodlbauer, Matthias Schmal, Christian Waltl, Thomas Rohr, Astrid R Mach-Aigner, Marko D Mihovilovic, Florian Rudroff
{"title":"Unlocking the potential of cyanobacteria: a high-throughput strategy for enhancing biocatalytic performance through genetic optimization.","authors":"Julia Jodlbauer, Matthias Schmal, Christian Waltl, Thomas Rohr, Astrid R Mach-Aigner, Marko D Mihovilovic, Florian Rudroff","doi":"10.1016/j.tibtech.2024.07.011","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.07.011","url":null,"abstract":"<p><p>Cyanobacteria show promise as hosts for whole-cell biocatalysis. Their photoautotrophic metabolism can be leveraged for a sustainable production process. Despite advancements, performance still lags behind heterotrophic hosts. A key challenge is the limited ability to overexpress recombinant enzymes, which also hinders their biocatalytic efficiency. To address this, we generated large-scale expression libraries and developed a high-throughput method combining fluorescence-activated cell sorting (FACS) and deep sequencing in Synechocystis sp. PCC 6803 (Syn. 6803) to screen and optimize its genetic background. We apply this approach to enhance expression and biocatalyst performance for three enzymes: the ketoreductase LfSDR1M50, enoate reductase YqjM, and Baeyer-Villiger monooxygenase (BVMO) CHMO<sub>mut</sub>. Diverse genetic combinations yielded significant improvements: optimizing LfSDR1M50 expression showed a 17-fold increase to 39.2 U g<sub>cell dry weight (CDW)</sub><sup>-1</sup>. In vivo activity of Syn. YqjM was improved 16-fold to 58.7 U g<sub>CDW</sub><sup>-1</sup> and, for Syn. CHMO<sub>mut</sub>, a 1.5-fold increase to 7.3 U g<sub>CDW</sub><sup>-1</sup> was achieved by tailored genetic design. Thus, this strategy offers a pathway to optimize cyanobacteria as expression hosts, paving the way for broader applications in other cyanobacteria strains and larger libraries.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reducing education inequalities through cloud-enabled live-cell biotechnology. 通过云端活细胞生物技术减少教育不平等。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-28 DOI: 10.1016/j.tibtech.2024.07.015
Samira Vera-Choqqueccota, Baha Eddine Youcef Belmekki, Mohamed-Slim Alouini, Mircea Teodorescu, David Haussler, Mohammed A Mostajo-Radji
{"title":"Reducing education inequalities through cloud-enabled live-cell biotechnology.","authors":"Samira Vera-Choqqueccota, Baha Eddine Youcef Belmekki, Mohamed-Slim Alouini, Mircea Teodorescu, David Haussler, Mohammed A Mostajo-Radji","doi":"10.1016/j.tibtech.2024.07.015","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.07.015","url":null,"abstract":"<p><p>Biotechnology holds the potential to drive innovations across various fields from agriculture to medicine. However, despite numerous interventions, biotechnology education remains highly unequal worldwide. Historically, the high costs and potential exposure to hazardous materials have hindered biotechnology education. Integration of cloud technologies into classrooms has emerged as an alternative solution that is already enabling biotechnology experiments to reach thousands of students globally. We describe several innovations that collectively facilitate real-time experimentation in biotechnology education in remote locations. These advances enable remote access to scientific data and live experiments, promote collaborative research, and ensure educational inclusivity. We propose cloud-enabled live-cell biotechnology as a mechanism for reducing inequalities in biotechnology education and promoting sustainable development.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Healing wounds with plasma-activated hydrogel therapy. 利用血浆活化水凝胶疗法愈合伤口。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-28 DOI: 10.1016/j.tibtech.2024.07.013
Sumyea Sabrin, Sung-Ha Hong, Debabrata K Karmokar, Habibullah Habibullah, Robert Fitridge, Robert D Short, Endre J Szili
{"title":"Healing wounds with plasma-activated hydrogel therapy.","authors":"Sumyea Sabrin, Sung-Ha Hong, Debabrata K Karmokar, Habibullah Habibullah, Robert Fitridge, Robert D Short, Endre J Szili","doi":"10.1016/j.tibtech.2024.07.013","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.07.013","url":null,"abstract":"<p><p>Chronic wound infections are a silent pandemic in danger of becoming a global healthcare crisis. Innovations to control infections and improve healing are required. In the context of this challenge, researchers are exploiting plasma-activated hydrogel therapy (PAHT) for use either alone or in combination with other antimicrobial strategies. PAHT involves the cold atmospheric pressure plasma activation of hydrogels with reactive oxygen and nitrogen species to decontaminate infections and promote healing. This opinion article describes PAHT for wound treatment and provides an overview of current research and outstanding challenges in translating the technology for medical use. A 'blueprint' of an autonomous PAHT is presented in the final section that can move the management and treatment of wounds from the clinical setting to the community.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioengineering lipid-based synthetic cells for therapeutic protein delivery. 用于输送治疗性蛋白质的脂质合成细胞生物工程。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-28 DOI: 10.1016/j.tibtech.2024.08.004
Sónia Siquenique, Shanny Ackerman, Avi Schroeder, Bruno Sarmento
{"title":"Bioengineering lipid-based synthetic cells for therapeutic protein delivery.","authors":"Sónia Siquenique, Shanny Ackerman, Avi Schroeder, Bruno Sarmento","doi":"10.1016/j.tibtech.2024.08.004","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.08.004","url":null,"abstract":"<p><p>Synthetic cells (SCs) offer a promising approach for therapeutic protein delivery, combining principles from synthetic biology and drug delivery. Engineered to mimic natural cells, SCs provide biocompatibility and versatility, with precise control over their architecture and composition. Protein production is essential in living cells, and SCs aim to replicate this process using compartmentalized cell-free protein synthesis systems within lipid bilayers. Lipid bilayers serve as favored membranes in SC design due to their similarity to the biological cell membrane. Moreover, engineering lipidic membranes enable tissue-specific targeting and immune evasion, while stimulus-responsive SCs allow for triggered protein production and release. This Review explores lipid-based SCs as platforms for therapeutic protein delivery, discussing their design principles, functional attributes, and translational challenges and potential.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harmonizing the symphony of chimeric antigen receptor T cell immunotherapy with the elegance of biomaterials. 将嵌合抗原受体 T 细胞免疫疗法的交响乐与生物材料的优雅相协调。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-23 DOI: 10.1016/j.tibtech.2024.07.017
Zhaozhao Chen, Yu Hu, Heng Mei
{"title":"Harmonizing the symphony of chimeric antigen receptor T cell immunotherapy with the elegance of biomaterials.","authors":"Zhaozhao Chen, Yu Hu, Heng Mei","doi":"10.1016/j.tibtech.2024.07.017","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.07.017","url":null,"abstract":"<p><p>Chimeric antigen receptor T cell (CAR-T) immunotherapy has become a heated field of cancer research, demonstrating revolutionary efficacy in refractory and relapsed hematologic malignancies. However, CAR-T therapy has still encountered tough challenges, including complicated and lengthy manufacturing procedures, mediocre targeted delivery, limited therapeutic effect against solid tumors and difficulties in real-time in vivo monitoring. To overcome these limitations, various versatile biomaterials have been used in the above aspects and have improved CAR-T therapy impressively. This review mainly summarizes the latest research progress of biomaterials promoting CAR-T therapy in manufacturing, enhancing targeted delivery and tumor infiltration, and dramatic in vivo tracking to provide new insights and inspiration for clinical treatment.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Programming mammalian cell behaviors by physical cues. 通过物理线索编程哺乳动物细胞行为
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2024-08-22 DOI: 10.1016/j.tibtech.2024.07.014
Jinbo Huang, Martin Fussenegger
{"title":"Programming mammalian cell behaviors by physical cues.","authors":"Jinbo Huang, Martin Fussenegger","doi":"10.1016/j.tibtech.2024.07.014","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.07.014","url":null,"abstract":"<p><p>In recent decades, the field of synthetic biology has witnessed remarkable progress, driving advances in both research and practical applications. One pivotal area of development involves the design of transgene switches capable of precisely regulating specified outputs and controlling cell behaviors in response to physical cues, which encompass light, magnetic fields, temperature, mechanical forces, ultrasound, and electricity. In this review, we delve into the cutting-edge progress made in the field of physically controlled protein expression in engineered mammalian cells, exploring the diverse genetic tools and synthetic strategies available for engineering targeting cells to sense these physical cues and generate the desired outputs accordingly. We discuss the precision and efficiency limitations inherent in these tools, while also highlighting their immense potential for therapeutic applications.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142047258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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