Trends in biotechnology最新文献_第6页

A combinatorial construct library enables an expanded expression range of secreted therapeutic proteins by probiotic yeast. 组合构建库使益生菌酵母菌分泌的治疗性蛋白的表达范围扩大。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-11 DOI: 10.1016/j.tibtech.2026.08.012

Miranda J Wallace, Christina Kratschmer, Brandon Brazil, Ilham Matiker, Esse M Evbuomwan, Jerome Prusa, Jasmine Najari, Rehan Mehta, Ana Romani, Sophia Zhang, Jie Ning, Ryan A Hagenson, James Liao, Bin Wang, Beth A Helmink, Gautam Dantas

{"title":"A combinatorial construct library enables an expanded expression range of secreted therapeutic proteins by probiotic yeast.","authors":"Miranda J Wallace, Christina Kratschmer, Brandon Brazil, Ilham Matiker, Esse M Evbuomwan, Jerome Prusa, Jasmine Najari, Rehan Mehta, Ana Romani, Sophia Zhang, Jie Ning, Ryan A Hagenson, James Liao, Bin Wang, Beth A Helmink, Gautam Dantas","doi":"10.1016/j.tibtech.2026.08.012","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.012","url":null,"abstract":"Orally administered engineered probiotics, including Saccharomyces cerevisiae var. boulardii (Sb), are of emerging interest as protein therapeutic delivery platforms to treat gastrointestinal diseases. Tools to readily optimize protein output are required to optimize the therapeutic index of Sb-produced therapies. In this study, a 125-plex Sb secretion construct library was developed consisting of all possible combinations of five promoters, five secretion signals, and five terminators, which enabled a greater than 1800-fold range in Sb expression of a Gaussia luciferase (GLuc) reporter. Secretion signal and promoter identities had significant effects on secretion output. This library further enabled a 28-fold improvement of binding activity of Sb-secreted haPD-1, an established anti-tumor immunotherapeutic, and improved haPD-1 detection in mouse stool samples following oral gavage of Sb_haPD-1. Sb secretion trends of both GLuc and haPD-1 in vitro mirrored payload expression in vivo. This protein secretion library toolkit will serve as a valuable resource to rapidly optimize protein therapeutic output from engineered Sb.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148945789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering biosensors to enhance monoterpene indole alkaloid production in yeast. 工程生物传感器提高单萜吲哚生物碱在酵母中的生产。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-09 DOI: 10.1016/j.tibtech.2026.08.011

Maxence Holtz, Simon D'Oelsnitz, Cecília Castellví Domingo, Niklas G Madsen, Mars Yu Hong, Jonathan Asmund Arnesen, Aafke C A van Aalst, Samantha de Haan, Christopher K Weingarten, Ditte H Welner, Pamela A Silver, Yan Jessie Zhang, Michael K Jensen, Carlos G Acevedo-Rocha

{"title":"Engineering biosensors to enhance monoterpene indole alkaloid production in yeast.","authors":"Maxence Holtz, Simon D'Oelsnitz, Cecília Castellví Domingo, Niklas G Madsen, Mars Yu Hong, Jonathan Asmund Arnesen, Aafke C A van Aalst, Samantha de Haan, Christopher K Weingarten, Ditte H Welner, Pamela A Silver, Yan Jessie Zhang, Michael K Jensen, Carlos G Acevedo-Rocha","doi":"10.1016/j.tibtech.2026.08.011","DOIUrl":"10.1016/j.tibtech.2026.08.011","url":null,"abstract":"Monoterpene indole alkaloids (MIAs) are medicinally valuable plant natural products, but their scarcity and structural complexity hinder conventional production. Microbial cell factories offer a sustainable alternative, although optimization of their long biosynthetic pathways is constrained by costly, low-throughput analytics. In this article, we evolved the promiscuous bacterial transcription factor RamR to detect five MIAs, obtaining sensitive and selective biosensor variants with EC50 values below 10 μM. We functionalized RamR in yeast and used it to screen a 188-gene overexpression library for improved strictosidine (STR) production, the common precursor for all MIAs. Biosensor fluorescence strongly correlated with HPLC measurements (R2 = 0.932), enabling identification of engineering targets that, when combined, increased STR titers more than threefold to over 220 mg/l. This RamR-based biosensing platform enables cost-effective screening and makes large biological design spaces experimentally accessible, accelerating strain and enzyme engineering for MIAs and other complex natural products.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrified future with microbial electrochemical technologies: how to realize the dream? 微生物电化学技术的电气化未来：如何实现梦想？

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-09 DOI: 10.1016/j.tibtech.2026.07.031

Sanne M de Smit, Largus Lars T Angenent, Federico Aulenta, Abraham Esteve-Nunez, Albert Guisasola, Falk Harnisch, Elizabeth S Heidrich, Ludovic Jourdin, Marika Kokko, Rikke Linssen, Deepak Pant, Catarina M Paquete, Jo Philips, Sebastià Puig, Korneel Rabaey, Miriam A Rosenbaum, Uwe Schröder, Tom Sleutels, César I Torres, Marianna Villano, Annemiek Ter Heijne

{"title":"An electrified future with microbial electrochemical technologies: how to realize the dream?","authors":"Sanne M de Smit, Largus Lars T Angenent, Federico Aulenta, Abraham Esteve-Nunez, Albert Guisasola, Falk Harnisch, Elizabeth S Heidrich, Ludovic Jourdin, Marika Kokko, Rikke Linssen, Deepak Pant, Catarina M Paquete, Jo Philips, Sebastià Puig, Korneel Rabaey, Miriam A Rosenbaum, Uwe Schröder, Tom Sleutels, César I Torres, Marianna Villano, Annemiek Ter Heijne","doi":"10.1016/j.tibtech.2026.07.031","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.07.031","url":null,"abstract":"Microbial electrochemical technologies (METs) are emerging with innovative applications in various fields by harnessing the combined action of electrodes and microbes. What if METs could be the key to circularity in wastewater treatment? What if METs could be employed to convert electricity and CO2, or waste, into valuable organics? What if new bioinspired materials could be produced through METs? What if METs could be used as smart bio-based sensors? We provide our vision for the future of METs in four sectors: water treatment, industrial biotechnology, electronics, and biosensors. We showcase why and how METs could advance or replace state-of-the-art technologies and discuss the challenges that must be overcome to make METs part of an electrified future powered by microbes.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A logic-gated synthetic circuit coupled with microenvironmental reprogramming overcomes immune exclusion in antigen-poor chondrosarcoma. 结合微环境重编程的逻辑门控合成电路克服了抗原贫乏软骨肉瘤的免疫排斥。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-07 DOI: 10.1016/j.tibtech.2026.08.008

Jooyeon Suh, Geunho Yook, Junhan Bae, Sehan Jeong, Somin Kim, June Hyuk Kim, Hyun Guy Kang, Ming-Ru Wu, Yi-Jun Kim, Jin-Hong Kim

{"title":"A logic-gated synthetic circuit coupled with microenvironmental reprogramming overcomes immune exclusion in antigen-poor chondrosarcoma.","authors":"Jooyeon Suh, Geunho Yook, Junhan Bae, Sehan Jeong, Somin Kim, June Hyuk Kim, Hyun Guy Kang, Ming-Ru Wu, Yi-Jun Kim, Jin-Hong Kim","doi":"10.1016/j.tibtech.2026.08.008","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.008","url":null,"abstract":"Chondrosarcoma remains refractory to immunotherapy due to a paucity of targetable antigens and a suppressive tumor microenvironment (TME). We developed a modular strategy integrating tumor-intrinsic genetic logic with rational TME reprogramming to overcome these barriers. Using a 57,715-element massively parallel reporter assay, we identified chondrosarcoma-selective synthetic promoters and engineered a Boolean AND-gate circuit that ensures high-fidelity expression of a surface T cell engager exclusively within malignant cells. To further address the immunosuppressive TME, we identified macrophage migration inhibitory factor (MIF) as a target for blockade. Single-cell RNA sequencing in human PBMC-reconstituted chondrosarcoma xenograft models revealed that the circuit initiates T cell activation, while MIF blockade prevents exhaustion by promoting proliferative T cells and M1-polarized macrophages. Local administration induced a systemic abscopal effect, regressing distant, untreated tumors. Collectively, these findings provide a programmable blueprint for converting immunologically cold, antigen-poor malignancies into systemically immune-responsive states.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148907299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensing the future of cell therapy manufacturing. 感知细胞治疗制造的未来。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-03 DOI: 10.1016/j.tibtech.2026.08.010

Masoud Khazaei, Muamer Dervisevic, Nicolas H Voelcker

引用次数: 0

Beyond statutory compliance: toward comprehensive genomic governance. 超越法规遵从：迈向全面的基因组治理。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-03 DOI: 10.1016/j.tibtech.2026.08.009

Guillermo Aquino-Jarquin

引用次数: 0

Advancing European standards and regulations for 3D bioprinting. 推进欧洲3D生物打印标准和法规。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-03 DOI: 10.1016/j.tibtech.2026.08.001

Ambra Maddalon, Giulia Leonetti, Sophie Zimmer

引用次数: 0

Biotechnology-driven artificial diets for mass-rearing arthropod natural enemies. 大规模饲养节肢动物天敌的生物技术驱动人工饲料。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-01-22 DOI: 10.1016/j.tibtech.2025.12.010

Yong Wang, Wei-Cheng Yang, Patrick De Clercq, Norman C Leppla, Adeney de Freitas Bueno, Ricardo Ramirez-Romero, Nicolas Desneux, Lian-Sheng Zang

引用次数: 0

Multifunctional biosensors redefining liquid biopsy: advancing simultaneous multianalyte detection. 多功能生物传感器重新定义液体活检：推进同步多分析物检测。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-02-14 DOI: 10.1016/j.tibtech.2025.12.026

Panagiota M Kalligosfyri, Antonella Miglione, Ada Raucci, Wanda Cimmino, Gabriella Iula, Alessandra Glovi, Sima Singh, Michelino De Laurentiis, Antonio Giordano, Stefano Cinti

{"title":"Multifunctional biosensors redefining liquid biopsy: advancing simultaneous multianalyte detection.","authors":"Panagiota M Kalligosfyri, Antonella Miglione, Ada Raucci, Wanda Cimmino, Gabriella Iula, Alessandra Glovi, Sima Singh, Michelino De Laurentiis, Antonio Giordano, Stefano Cinti","doi":"10.1016/j.tibtech.2025.12.026","DOIUrl":"10.1016/j.tibtech.2025.12.026","url":null,"abstract":"Liquid biopsy has revolutionized noninvasive disease diagnosis and monitoring by detecting circulating biomarkers such as tumor-derived DNA, miRNAs, proteins, exosomes, and circulating tumor cells in body fluids. Conventional single-analyte approaches fail to capture disease complexity, limiting diagnostic accuracy. Multifunctional biosensors enable simultaneous detection of multiple biomarkers, representing a paradigm shift in liquid biopsy. This review highlights recent advances in diverse detection strategies, including optical, electrochemical, plasmonic, and clustered regularly interspaced short palindromic repeats-based assays, alongside innovations in hybrid platforms, smart surface engineering, and nanotechnology. We also discuss integration with microfluidics, artificial intelligence, and chemometric analysis to enhance sensitivity, reduce signal interference, and improve scalability. Finally, challenges in clinical translation and standardization are highlighted, underscoring the transformative potential of multifunctional biosensors in precision medicine and early disease detection.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2577-2595"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146198116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermotolerant yeasts and their biotechnological applications. 耐热酵母及其生物技术应用。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-02-23 DOI: 10.1016/j.tibtech.2025.12.025

Roksolana Vasylyshyn, Justyna Ruchala, Kostyantyn Dmytruk, Andriy Sibirny

引用次数: 0