Trends in biotechnology最新文献_第10页

Creating a glycolysis-minimized central metabolism for efficient biosynthesis and cell growth in yeast. 在酵母中创造糖酵解最小化的中心代谢，以实现高效的生物合成和细胞生长。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-17 DOI: 10.1016/j.tibtech.2026.07.020

Xin Ni, Haoyu Wang, Mengyao Zhang, Ning Gao, Jiaoqi Gao, Fan Yang, Yongjin J Zhou

{"title":"Creating a glycolysis-minimized central metabolism for efficient biosynthesis and cell growth in yeast.","authors":"Xin Ni, Haoyu Wang, Mengyao Zhang, Ning Gao, Jiaoqi Gao, Fan Yang, Yongjin J Zhou","doi":"10.1016/j.tibtech.2026.07.020","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.07.020","url":null,"abstract":"Long-term natural evolution has selected glycolysis as the major metabolic mode to generate energy and cellular building blocks for rapid cell growth in eukaryotes such as yeast; however, glycolysis lacks a sufficient NADPH supply to drive the biosynthesis of reduced chemicals. Industrially feasible cell factories often require the most energy-economical pathway for the high-level production of target products. In this study, we successfully established a pentose phosphate pathway-dominant, glycolysis-minimized central metabolic mode in yeast, which significantly improved the cellular energy status compared with glycolysis and provided a balanced supply of ATP and NADPH. More importantly, we discovered a global carbon metabolism regulator, which drives metabolic flux toward glycolysis for energy generation, and whose disruption relieved the tight regulation of metabolic flux distribution and significantly enhanced the cellular energy status, which in turn improved the production of the energy-intensive molecules free fatty acids (FFAs) by 63%. The final engineered strain produced FFAs at a titer of 41.7 g/l, the highest titer reported for yeast fermentation. Our work provides valuable insights into the metabolic regulatory mechanisms of microorganisms.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From liquid precursors to adhesive platforms: coacervates as dynamic interface biomaterials for biotechnology. 从液体前体到黏附平台：凝聚体作为生物技术的动态界面生物材料。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-17 DOI: 10.1016/j.tibtech.2026.07.028

Margarida M A Sacramento, Luís Henrique Reichembach, João M M Rodrigues, João F Mano

引用次数: 0

On-target and off-target activities of CRISPR therapeutics across scales. 跨尺度CRISPR疗法的靶标和脱靶活性。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-17 DOI: 10.1016/j.tibtech.2026.07.015

Merna Magdy, Justin R Gibson, Abishek Dhungana, Ashley Herring-Nicholas, Renee N Cottle, Eric A Josephs

{"title":"On-target and off-target activities of CRISPR therapeutics across scales.","authors":"Merna Magdy, Justin R Gibson, Abishek Dhungana, Ashley Herring-Nicholas, Renee N Cottle, Eric A Josephs","doi":"10.1016/j.tibtech.2026.07.015","DOIUrl":"10.1016/j.tibtech.2026.07.015","url":null,"abstract":"Recent FDA-approved gene-editing therapies illustrate not only the transformative potential of biotechnologies using CRISPR (clustered regularly interspaced short palindromic repeats)-derived ribonucleoproteins in treating a broad range of diseases but also the spectrum of possible molecular variations CRISPR therapeutics can adopt. These include exagamglogene autotemcel, an ex vivo therapy for hemoglobinopathies using CRISPR nuclease Cas9, and kayjayguran abengcemeran, an in vivo therapy using a protospacer adjacent motif-altered base-editing Cas9 variant for an ultra-rare metabolic disorder. Together, these therapies underscore how far CRISPR has advanced beyond its original use as a tool in biological/biomedical research. In this opinion article, we argue that as CRISPR biotechnologies advance beyond the relative simplicity of in vitro applications, our understanding must also evolve to address the challenges of optimizing 'on-target' and 'off-target' mutational activities across the diverse contexts in which they occur.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13509143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation of cell-based therapies with enhanced cell viability and therapeutic efficacy. 细胞冷冻保存疗法提高细胞活力和治疗效果。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-17 DOI: 10.1016/j.tibtech.2026.07.019

Amy H Lee, Khanh T M Tran, Alexander Hostetler, Kewen Lei, Jinbi Tian, Sevinj Mursalova, Alicia Lau, Gary W Liu, Charles Gorelick, Darrell J Irvine, Robert Langer, Ana Jaklenec

{"title":"Cryopreservation of cell-based therapies with enhanced cell viability and therapeutic efficacy.","authors":"Amy H Lee, Khanh T M Tran, Alexander Hostetler, Kewen Lei, Jinbi Tian, Sevinj Mursalova, Alicia Lau, Gary W Liu, Charles Gorelick, Darrell J Irvine, Robert Langer, Ana Jaklenec","doi":"10.1016/j.tibtech.2026.07.019","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.07.019","url":null,"abstract":"Biobanking has played an important role in advancing cell therapy by enabling long-term preservation of cell function, facilitating product transport, and allowing flexibility in infusion scheduling. However, conventional cryopreservation methods rely on high concentrations of dimethyl sulfoxide (DMSO) and serum, which can lead to toxicity, compromise cell viability, complicate post-thaw handling (such as DMSO removal), require additional quality testing, and ultimately reduce therapeutic efficacy. We present a cryopreservation strategy that uses electroporation to introduce protective sugars intracellularly, termed sugar augmented freezing by electroporation (SAFE). Applied across multiple cell types-including chimeric antigen receptor T cells and stem cells-SAFE improved post-thaw viability and expansion by 1.8-fold and enhanced therapeutic efficacy by two-fold compared with standard cryopreservation. Furthermore, SAFE preserved proliferation-associated proteins and metabolites more effectively. Post-thaw cells maintained, and often exceeded, the functional performance of conventionally preserved cells. This work highlights the importance of optimizing cryogenic parameters to achieve greater post-thaw consistency, scalability, and clinical impact on next-generation cell-based therapies.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ programming of unconventional T cells. 非常规T细胞的原位编程。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-14 DOI: 10.1016/j.tibtech.2026.07.027

Yan-Ruide Li, Haochen Nan, Youcheng Yang, Shubing Wang, Lili Yang

引用次数: 0

Empowering microbes with natural armor: encapsulation strategies for agri-food and biotech innovations. 赋予微生物天然盔甲：农业食品和生物技术创新的封装策略。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-14 DOI: 10.1016/j.tibtech.2026.07.030

Simona Cioffi, Michele Iafisco, Alessio Adamiano

引用次数: 0

Precision-fermented milk proteins as a model for industrial food biotechnology. 精密发酵乳蛋白作为工业食品生物技术的典范。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-13 DOI: 10.1016/j.tibtech.2026.07.026

Mathilde Pinon, Per Falholt, Thomas G Schmidt, José Arnau

引用次数: 0

Pilot-scale production of leucine from CO₂. 从二氧化碳中生产亮氨酸的中试规模。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-13 DOI: 10.1016/j.tibtech.2026.07.014

Christian Fink, Franziska Steger, Sebastian Schmidt, Elisa Hilts, François Unger, Aquilla Ruddyard, Maximilian Klein, Bart-Jan Akerboom, Thomas Stehrer-Polašek, Stefan Drießler, Shubham Gurav, Ross T Fennessy, Justin Smith, Günther Bochmann, Simon K-M R Rittmann

{"title":"Pilot-scale production of leucine from CO2.","authors":"Christian Fink, Franziska Steger, Sebastian Schmidt, Elisa Hilts, François Unger, Aquilla Ruddyard, Maximilian Klein, Bart-Jan Akerboom, Thomas Stehrer-Polašek, Stefan Drießler, Shubham Gurav, Ross T Fennessy, Justin Smith, Günther Bochmann, Simon K-M R Rittmann","doi":"10.1016/j.tibtech.2026.07.014","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.07.014","url":null,"abstract":"Beyond their nutritional value, amino acids are of relevance in medicine and, due to their chemical properties, they are indispensable for applications in many different realms, such as pharmaceutics, cosmetics, animal feed, food, and the beverage industry. In this research article, we report an archaeal cell factory for leucine production from CO2 that has been generated by rational design, random mutagenesis, and pathway engineering. The cell factory has been bioprocess-technologically examined and successfully scaled up with regard to productivity, product quality, and operational stability. In a 2-day fed-batch campaign, we produced 181 g of leucine from CO2 at the 150-L pilot-plant scale with a mean volumetric leucine productivity of 65 mg L-1 h-1. A thorough techno-economic analysis indicates that the rollout of leucine production from CO2 is nearly economically feasible on a global scale.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148763963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Bacillus thuringiensis ΔcdsR-based expression system for insecticidal proteins identified using AlphaFold3. 利用AlphaFold3鉴定苏云金芽孢杆菌ΔcdsR-based杀虫蛋白表达系统。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-12 DOI: 10.1016/j.tibtech.2026.07.023

Shiqing Li, Xin Zhang, Tinglu Yan, Yabin Liu, Yuhan Chen, Zihan Zhang, Yuanhong Zhu, Yanrong Xu, Yang Luo, Yang Liu, Neil Crickmore, Dong Fan, Fuping Song

{"title":"A Bacillus thuringiensis ΔcdsR-based expression system for insecticidal proteins identified using AlphaFold3.","authors":"Shiqing Li, Xin Zhang, Tinglu Yan, Yabin Liu, Yuhan Chen, Zihan Zhang, Yuanhong Zhu, Yanrong Xu, Yang Luo, Yang Liu, Neil Crickmore, Dong Fan, Fuping Song","doi":"10.1016/j.tibtech.2026.07.023","DOIUrl":"10.1016/j.tibtech.2026.07.023","url":null,"abstract":"Environmentally friendly biopesticides, such as those based on the bacterium Bacillus thuringiensis (Bt), have an important role to play in Integrated Pest Management systems. An increasingly diverse range of pesticidal proteins is being discovered, led by advances in genome sequencing and the bioinformatic identification of candidate genes. In this work, we utilized an AlphaFold3-based structure-prediction approach to identify candidate genes and, in parallel, developed a Bt chassis strain that can be universally used to express new pesticidal proteins. This chassis strain is based on a cdsR deletion strain with a controllable cell death pathway that decouples toxin production from sporulation, enabling cytoplasmic hyperexpression via strong promoters and encapsulation of recombinant proteins. These characteristics provide superior efficacy, UV stability, and environmental safety compared with conventional Bt formulations. Several of the identified proteins displayed insecticidal activity when expressed in this strain, providing a versatile framework for next-generation biocontrol and sustainable pest-management innovation.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148725252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scalable 4D biofabrication process for the manufacture of scaffold-free bone-forming callus implants. 无支架骨形成愈伤组织植入物的可扩展4D生物制造工艺。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-12 DOI: 10.1016/j.tibtech.2026.07.021

Andreas Dimopoulos, Konstantinos Ioannidis, Nikolaos S Giakoumakis, Hanna Svitina, Charalampos Androulidakis, Dirk De Vos, Isaak Decoene, Ioannis Papantoniou

{"title":"Scalable 4D biofabrication process for the manufacture of scaffold-free bone-forming callus implants.","authors":"Andreas Dimopoulos, Konstantinos Ioannidis, Nikolaos S Giakoumakis, Hanna Svitina, Charalampos Androulidakis, Dirk De Vos, Isaak Decoene, Ioannis Papantoniou","doi":"10.1016/j.tibtech.2026.07.021","DOIUrl":"10.1016/j.tibtech.2026.07.021","url":null,"abstract":"Critical-size long bone defects remain a major clinical challenge, with treatments such as autografts or distraction osteogenesis causing donor-site morbidity, infection, or failure to restore complex bone architecture. Tissue-engineered implants that recapitulate native fracture healing provide a promising solution. However, scalability for dense cellular constructs is lacking. To address this, we bioprinted high-cell-density implants using rheologically competent sacrificial alginate bioinks. The constructs were supported by partially crosslinked alginate during bioprinting and chondrogenic differentiation, after which selective EDTA-mediated alginate dissolution generated scaffold-free implants. Quality characterization confirmed chondro-osteogenic signatures and extracellular matrix gene upregulation. Upon in vivo implantation in immunocompromised mice, implants underwent endochondral ossification, forming cortical and trabecular bone with bone marrow compartments. Integration with a suspension bioreactor enabled production of human-sized proof-of-concept implants. This work establishes a scalable 4D biofabrication process that integrates 3D bioprinting with dissolvable sacrificial alginate bioinks and results in scaffold-free, bone-forming callus implants.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148723777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0