Trends in biotechnology最新文献_第9页

Precision public engagement: charting a path forward for biotechnology. 精准的公众参与：为生物技术指明前进的道路。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-31 DOI: 10.1016/j.tibtech.2026.08.004

Anna C F Lewis, Naomi Scheinerman

引用次数: 0

NAMs without context of use slow EU adoption. 没有使用背景的域名减慢了欧盟的采用。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-27 DOI: 10.1016/j.tibtech.2026.08.005

Marco Straccia, Milena Mennecozzi

引用次数: 0

Promoter selection reduces phenotypic heterogeneity and improves bioproduction. 启动子选择减少了表型异质性，提高了生物产量。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-27 DOI: 10.1016/j.tibtech.2026.08.002

Davina Patel, Kiyan Shabestary, Jiabao Xu, Cinzia Klemm, Wei E Huang, Rodrigo Ledesma-Amaro

{"title":"Promoter selection reduces phenotypic heterogeneity and improves bioproduction.","authors":"Davina Patel, Kiyan Shabestary, Jiabao Xu, Cinzia Klemm, Wei E Huang, Rodrigo Ledesma-Amaro","doi":"10.1016/j.tibtech.2026.08.002","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.002","url":null,"abstract":"Cell-to-cell heterogeneity leads to the formation of subpopulations within an isogenic population, resulting in the coexistence of high and low producer cells. However, the impact of this phenotypic heterogeneity on bioproduction has been overlooked. Using flow cytometry to analyse commonly used yeast promoters, we found that many display macro-heterogeneity, generating multiple discrete subpopulations. Additionally, by regressing single-cell protein expression on single-cell RNA sequencing data, we explored the use of predictive models as a guide for selecting promoters with more homogeneous expression at desired expression levels. As a proof of concept, we engineered an improved lycopene pathway by substituting homogeneous promoters for macro-heterogeneous ones and implemented single-cell Raman spectroscopy to demonstrate up to a threefold increase in single-cell and bulk lycopene production. Overall, our results show that promoter heterogeneity can have a large impact on microbial bioprocesses and that the selection of homogeneous promoters can achieve more efficient production in a metabolically engineered pathway.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148832737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organ-on-chip bridging musculoskeletal disease modeling and precision therapeutic discovery. 器官芯片桥接肌肉骨骼疾病建模和精确治疗发现。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-27 DOI: 10.1016/j.tibtech.2026.08.003

Qi Gao, Max L Lee, Meagan Makarczyk, Simon K-H Chow, Bruce A Bunnell, Hang Lin, Stuart B Goodman

引用次数: 0

Bioinspired microvascular networks for stroke recovery, cerebral revascularization, and neuroprotection. 生物激发微血管网络用于中风恢复、脑血运重建和神经保护。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-26 DOI: 10.1016/j.tibtech.2026.07.035

Yuke Xie, Peng Lu, Xiaogang Tang, Cong Yao, Bingsong Gu, Xiaoli Qu, Xianhui Zhang, Yijing He, Yabo Zhang, Zhishuo Ren, Dichen Li, Jiankang He, Mao Mao, Jianhua Peng, Yong Jiang

{"title":"Bioinspired microvascular networks for stroke recovery, cerebral revascularization, and neuroprotection.","authors":"Yuke Xie, Peng Lu, Xiaogang Tang, Cong Yao, Bingsong Gu, Xiaoli Qu, Xianhui Zhang, Yijing He, Yabo Zhang, Zhishuo Ren, Dichen Li, Jiankang He, Mao Mao, Jianhua Peng, Yong Jiang","doi":"10.1016/j.tibtech.2026.07.035","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.07.035","url":null,"abstract":"Cerebral ischemic stroke remains a leading cause of disability, with limited options for restoring cerebral blood flow (CBF) and promoting recovery. This study introduces a leaf-mimetic microvascular network to promote poststroke revascularization. The network uses leaf-venation-inspired microchannel templates to direct cell-hydrogel self-assembly into microvascular networks. In a photothrombotic stroke model, the implanted network integrates with host vessels, enhances neovascularization, improves CBF, reduces infarct volume, improves neuronal survival, and promotes functional recovery. Transcriptomic and histological analyses suggest enrichment of potentially reparative neutrophils and reduced inflammation associated with neutrophil extracellular traps as components of the network-associated immune-vascular response. Blocking neutrophil recruitment attenuates network-mediated neovascularization, whereas local delivery of potentially reparative neutrophils partially restores vascular remodeling. These findings provide a preclinical proof of concept that bioinspired microvascular constructs can promote cerebral revascularization and neuroprotection after stroke, while identifying immune-vascular processes that warrant further mechanistic and translational validation.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148832729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erythrocytes as programmable intravascular interfaces for therapeutic delivery. 红细胞作为治疗递送的可编程血管内接口。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-19 DOI: 10.1016/j.tibtech.2026.07.034

Min Zhang, Fei Chen

引用次数: 0

Engineering RNA-guided bridge recombinases for precise large-scale genome editing. 用于精确大规模基因组编辑的工程rna引导桥接重组酶。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-19 DOI: 10.1016/j.tibtech.2026.07.025

Rui Gao, Jingjing Wei, Chao Sun, Jinxing Liu, Zhenyu Li, Yunjia Li, Qiang Gao, Kecheng Yang, Caixia Gao

{"title":"Engineering RNA-guided bridge recombinases for precise large-scale genome editing.","authors":"Rui Gao, Jingjing Wei, Chao Sun, Jinxing Liu, Zhenyu Li, Yunjia Li, Qiang Gao, Kecheng Yang, Caixia Gao","doi":"10.1016/j.tibtech.2026.07.025","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.07.025","url":null,"abstract":"Precise manipulation of large DNA fragments in eukaryotic genomes remains limited by the low efficiency and delivery constraints of current multicomponent editing systems. In this study, we engineered an RNA-guided bridge recombinase system through rational mutagenesis and AI-assisted directed evolution, enabling programmable chromosomal rearrangements in both plant and mammalian cells and achieving up to a 29.8-fold increase in activity. In plants, the optimized system mediated precise deletions, insertions, and inversions from 1.8- to 315-kb DNA fragments, with stable editing efficiencies of up to 23.9% in regenerated rice plants. We further generated herbicide-resistant rice through a 315-kb chromosomal inversion that rewired endogenous promoter activity. In mammalian cells, the compact ISCro4 recombinase system was delivered using a single adeno-associated virus vector, thereby supporting efficient genome editing. Together, these results establish bridge RNA-guided recombinases as a versatile platform for programmable chromosome-scale genome engineering, with broad potential for precision breeding and gene therapy.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building a biotechnology company? Check this Indication Checklist. 建立一家生物技术公司？检查这一指示清单。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-19 DOI: 10.1016/j.tibtech.2026.07.033

William N Hait

引用次数: 0

Programmable biofunctionalization of bacterial cellulose via a dynamic co-culture platform. 通过动态共培养平台实现细菌纤维素的可编程生物功能化。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-19 DOI: 10.1016/j.tibtech.2026.07.032

Fei Liu, Yanyi Wang, Bin Cui, Yanwen Feng, Jun Yue, Chao Zhong

{"title":"Programmable biofunctionalization of bacterial cellulose via a dynamic co-culture platform.","authors":"Fei Liu, Yanyi Wang, Bin Cui, Yanwen Feng, Jun Yue, Chao Zhong","doi":"10.1016/j.tibtech.2026.07.032","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.07.032","url":null,"abstract":"Programmable biofunctionalization of bacterial cellulose (BC) is promising for constructing engineered living materials, but current microbial co-culture approaches struggle to combine coherent matrix formation, dynamic cultivation, and efficient matrix-associated protein functionalization. In this study, we report a polydopamine (PDA)-mediated, surface-immobilized dynamic co-culture platform that pairs Komagataeibacter rhaeticus for BC production with engineered Pichia pastoris for recombinant protein secretion. PDA-mediated co-immobilization preserves conformal BC growth under agitation while maintaining the functional partner near the forming matrix. Cellulose-binding-domain fusion further retains secreted protein cargoes within the BC network, enabling localized functionalization. The template-guided strategy supports geometry control, construct-size scaling, and incorporation of multiple engineered yeast populations. Modular genetic payloads enable pollutant degradation and enzymatic cascade-based analyte detection. As an application-relevant demonstration, lysostaphin-functionalized BC hydrogels effectively controlled Staphylococcus aureus infection and accelerated wound closure in a diabetic murine model. This work establishes a functionally extensible framework for bioactive BC-based living materials.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Material agency: operationalizing the living/nonliving interface in engineered materials. 材料代理：工程材料中生物/非生物界面的操作化。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-08-18 DOI: 10.1016/j.tibtech.2026.07.036

Andrea Belluati

引用次数: 0