Trends in biotechnology最新文献_第9页

Bridging the path to multiplexed molecular diagnostics. 架起通往多路分子诊断的桥梁。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-22 DOI: 10.1016/j.tibtech.2025.07.032

Cansu Pinar Yenice, Ciara K O'Sullivan

引用次数: 0

Advanced mass spectrometry techniques for monitoring biopharmaceutical host cell proteins. 用于监测生物制药宿主细胞蛋白的先进质谱技术。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-22 DOI: 10.1016/j.tibtech.2025.07.026

Paresh Tank, Shruti Vora, Fatima D'Souza, Shashank Shivaji Kamble, Neelamegam Rameshkumar

引用次数: 0

Strengthening the competitiveness of EU in vitro biotechnologies. 加强欧盟体外生物技术的竞争力。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-20 DOI: 10.1016/j.tibtech.2025.07.019

Milena Mennecozzi, Monica Piergiovanni, Lucia Selfa Aspiroz, Maurice Whelan

引用次数: 0

Identifying local gradients in methane-producing biocathodes. 确定产甲烷生物阴极的局部梯度。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-15 DOI: 10.1016/j.tibtech.2025.07.022

Micaela Brandão Lavender, Jasper P Groot, Annemiek Ter Heijne, Sanne M de Smit

{"title":"Identifying local gradients in methane-producing biocathodes.","authors":"Micaela Brandão Lavender, Jasper P Groot, Annemiek Ter Heijne, Sanne M de Smit","doi":"10.1016/j.tibtech.2025.07.022","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.07.022","url":null,"abstract":"CH4-producing bioelectrochemical systems (BES) are a promising alternative to convert CO2 and electricity into CH4. However, not much is known about the local conditions and possible gradients at CH4-producing biocathodes, especially when using granular activated carbon (GAC) as the electrode material. Detecting local conditions at different depths and heights of this 3D material provides better insights on possibly existing limitations. Process conditions and reactor design can be changed to tackle limitations and improve rates and efficiencies. Here, H2, pH, and oxidation reduction potentials (ORP) were measured locally within the biocathode. First, H2 was detected locally at -0.63 Vcathode potential (versus Ag/AgCl), whereas no H2 was detected in the outlet gas, suggesting efficient biological use of H2 as an intermediate. Second, gradients in all three parameters were observed at different depths in the biocathode. Hence, to improve biological activity, it is critical to consider H2 as a mediator and pH dead zones.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144862500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial production of D-mannose and D-sedoheptulose with tunable ratios. 可调比例d -甘露糖和d -糖庚糖的微生物生产。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-15 DOI: 10.1016/j.tibtech.2025.07.017

Dileep Sai Kumar Palur, Bryant Luu, Jayce E Taylor, Mohan Singhal, John Didzbalis, Justin B Siegel, Shota Atsumi

{"title":"Microbial production of D-mannose and D-sedoheptulose with tunable ratios.","authors":"Dileep Sai Kumar Palur, Bryant Luu, Jayce E Taylor, Mohan Singhal, John Didzbalis, Justin B Siegel, Shota Atsumi","doi":"10.1016/j.tibtech.2025.07.017","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.07.017","url":null,"abstract":"Rare sugars are valuable for food and pharmaceutical applications. D-Mannose, a low-calorie sweetener, is traditionally produced via chemical extraction from plant biomass, which is unsustainable, while enzymatic methods suffer from low yields due to equilibrium limitations. Here, we demonstrate that Escherichia coli can naturally synthesize D-mannose from D-glucose through a phosphorylation-isomerization-dephosphorylation pathway. We enhanced D-mannose production by deleting competing pathways and overexpressing key biosynthetic genes. Unexpectedly, due to the promiscuous activity of the phosphatase HxpB, which dephosphorylates both D-mannose-6-phosphate (M6P) and D-sedoheptulose-7-phosphate (S7P), the engineered strain also produced D-sedoheptulose, a non-sweet rare sugar that inhibits C6 sugar consumption. Further metabolic engineering improved D-sedoheptulose production. These optimizations enabled the development of a co-production strain capable of producing both sugars with tunable ratios. By leveraging this unique sugar combination, our approach provides a sustainable route to rare sugar biosynthesis and opens new possibilities for functional food design and metabolic regulation.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144862501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computationally guided genome rewiring of Escherichia coli and its application for nanopolyethylene terephthalate (PET) biodegradation and upcycling. 计算引导大肠杆菌基因组重组及其在纳米聚对苯二甲酸乙二醇酯（PET）生物降解和升级回收中的应用。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-14 DOI: 10.1016/j.tibtech.2025.07.008

Paula Vidal, Joan Giménez-Dejoz, Laura Fernandez-Lopez, Sonia Romero, Seyed Amirabbas Nazemi, Miguel Luengo, Jose L Gonzalez-Alfonso, Mireia Martinez-Sugrañes, Ana Robles-Martín, David Almendral, Sergi Roda, Pablo Pérez-García, Luzie Kruse, Karl-Erich Jaeger, Wolfgang R Streit, Francisco J Plou, Martin Floor, Patrick Shahgaldian, Rafael Bargiela, Víctor Guallar, Manuel Ferrer

{"title":"Computationally guided genome rewiring of Escherichia coli and its application for nanopolyethylene terephthalate (PET) biodegradation and upcycling.","authors":"Paula Vidal, Joan Giménez-Dejoz, Laura Fernandez-Lopez, Sonia Romero, Seyed Amirabbas Nazemi, Miguel Luengo, Jose L Gonzalez-Alfonso, Mireia Martinez-Sugrañes, Ana Robles-Martín, David Almendral, Sergi Roda, Pablo Pérez-García, Luzie Kruse, Karl-Erich Jaeger, Wolfgang R Streit, Francisco J Plou, Martin Floor, Patrick Shahgaldian, Rafael Bargiela, Víctor Guallar, Manuel Ferrer","doi":"10.1016/j.tibtech.2025.07.008","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.07.008","url":null,"abstract":"Numerous strategies for the biodegradation and upcycling of polyethylene terephthalate (PET) are under investigation. Here, we present a proof-of-concept study for reprogramming the Escherichia coli BL21(DE3) strain to degrade PET nanoparticles (nPET) without introducing foreign DNA and compromising native cellular fitness. In brief, native proteins selected in silico from the genome were repurposed to acquire artificial PETase activity without compromising their function and were subsequently replaced via CRISPR/Cas9 editing. A variant of the transport protein LsrB, selected for its ability to bind PET, was engineered to degrade PET powder (at 37-60°C). Building on LsrB periplasmic localization, we engineered a strain that degrades nPET at 37°C. The strain was further engineered to grow on nPET degradation products and produce valuable compounds. Our method, which is applicable across diverse genomes and microbial chassis, expands the potential of metabolic engineering to address plastic biodegradation and upcycling while reducing reliance on foreign DNA.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free screening of CRISPR-Cas activity by microfluidics-assisted in vitro compartmentalization. 利用微流体辅助体外区隔化技术对CRISPR-Cas活性进行无细胞筛选。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-14 DOI: 10.1016/j.tibtech.2025.07.007

Evgenios Bouzetos, Ketan Ashok Ganar, John van der Oost, Siddharth Deshpande

{"title":"Cell-free screening of CRISPR-Cas activity by microfluidics-assisted in vitro compartmentalization.","authors":"Evgenios Bouzetos, Ketan Ashok Ganar, John van der Oost, Siddharth Deshpande","doi":"10.1016/j.tibtech.2025.07.007","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.07.007","url":null,"abstract":"CRISPR-Cas systems are responsible for antiviral immunity of prokaryotic cells and have been repurposed as powerful genome-editing tools. Cell-free gene expression has been applied for the rapid characterization of CRISPR-Cas systems in microtiter plates. In vitro compartmentalization makes use of artificial microcompartments that individually act as bioreactors. Here, we performed cell-free reactions of CRISPR-Cas activity into microtiter plates, which we proceeded to encapsulate into double emulsion (DE) droplets generated by on-chip microfluidics. Emulsion droplets were screened for CRISPR-Cas activity based on relative fluorescence levels using a common cell sorter, and enrichment for the expected guide (g)RNA genotype was observed. Encapsulation of single gene copies per droplet is an important prerequisite for applying this technique to complex gene libraries. We show a proof-of-principle assay for efficient, compartmentalized gene amplification using magnetic microbeads. In conclusion, we demonstrate the feasibility of microfluidics-based, high-throughput, cell-free screening of CRISPR-Cas activity.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flux sampling and context-specific genome-scale metabolic models for biotechnological applications. 通量采样和环境特异性基因组尺度代谢模型用于生物技术应用。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-07 DOI: 10.1016/j.tibtech.2025.07.010

Devlin C Moyer, Justin Reimertz, Juan I Fuxman Bass, Daniel Segrè

引用次数: 0

Harnessing Streptomyces-plant interactions for agricultural natural product discovery. 利用链霉菌与植物的相互作用发现农业天然产品。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-07 DOI: 10.1016/j.tibtech.2025.07.018

Guozhong Du, Minghui Pan, Wensheng Xiang, Shanshan Li

引用次数: 0

Spatial omics for accelerating plant research and crop improvement. 加速植物研究和作物改良的空间组学。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-08-01 Epub Date: 2025-04-10 DOI: 10.1016/j.tibtech.2025.03.007

Rutwik Barmukh, Vanika Garg, Hao Liu, Annapurna Chitikineni, Liu Xin, Robert Henry, Rajeev K Varshney

引用次数: 0