Trends in biotechnology最新文献

Challenging plastic pollution with hydrocarbonoclastic lineages. 用碳氢化合物系挑战塑料污染。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-11-06 DOI: 10.1016/j.tibtech.2024.10.010

Yiqi Cao, Baiyu Zhang, Bing Chen

引用次数: 0

Advancing biopharmaceutical manufacturing: economic and sustainability assessment of end-to-end continuous production of monoclonal antibodies. 推进生物制药生产：单克隆抗体端到端连续生产的经济性和可持续性评估。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-11-06 DOI: 10.1016/j.tibtech.2024.10.007

Behnam Partopour, David Pollard

{"title":"Advancing biopharmaceutical manufacturing: economic and sustainability assessment of end-to-end continuous production of monoclonal antibodies.","authors":"Behnam Partopour, David Pollard","doi":"10.1016/j.tibtech.2024.10.007","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.10.007","url":null,"abstract":"Monoclonal antibodies (mAbs) have become essential therapeutics for treating various diseases. The robust, cost-effective, and sustainable production of mAbs is crucial due to their growing clinical and commercial demand. Advances in bioprocessing, such as improved cell lines, perfusion bioreactors, multicolumn chromatography, and automation, can significantly increase productivity, making treatments more accessible. Streamlining the production process also aligns with environmental sustainability by reducing waste and energy consumption. This study quantifies the economic and environmental impacts of incorporating recent advances into end-to-end continuous bioprocessing of mAbs. The results demonstrate that, compared with an optimized best-in-class fed-batch process (with 15 g/l titer and multicolumn chromatography), continuous manufacturing can reduce the total annual production costs, facility footprint, plastic waste, and CO2 emissions by up to 23%, 51%, 57%, and 54%, respectively, in a multiproduct facility producing clinical and commercial lots. Additionally, uncertainty analysis indicates that these gains are even more substantial under demand fluctuations.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chairside live biotherapeutic hydrogel for comprehensive periodontitis therapy. 用于牙周炎综合治疗的椅旁生物治疗水凝胶。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-11-05 DOI: 10.1016/j.tibtech.2024.10.001

Kaifeng Li, Boyi Li, Jiyun Li, Xiaoyi Wu, Yaning Zhao, Jian Yu, Jingmei Guo, Cui Huang

{"title":"Chairside live biotherapeutic hydrogel for comprehensive periodontitis therapy.","authors":"Kaifeng Li, Boyi Li, Jiyun Li, Xiaoyi Wu, Yaning Zhao, Jian Yu, Jingmei Guo, Cui Huang","doi":"10.1016/j.tibtech.2024.10.001","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.10.001","url":null,"abstract":"Periodontitis, characterized by microbial dysbiosis and immune dysregulation, destroys tooth-supporting tissues and negatively affects overall health. Current strategies face significant challenges in restoring damaged tissues while halting periodontitis progression. In this study, we introduce a live biotherapeutic product (LBP) in an engineered living hydrogel for comprehensive periodontitis therapy. A dental blue light-responsive hydrogel (LRG) was fabricated to deliver and confine live Lactobacillus rhamnosus GG (LGG) in periodontal pockets, endowing the LRG with sustained antibacterial and immunomodulatory effects. The LRG was engineered through peptide modification to also promote tissue regeneration. Both in vitro and in vivo evaluations confirmed the effectiveness of this integrated therapeutic strategy, which combines antibacterial, anti-inflammatory, and regenerative properties with an underlying immunomodulatory mechanism that involves suppressor of cytokine signaling (SOCS)3 upregulation and the Janus kinase/signal transducer and activator of transcription (JAK-STAT) pathway suppression in macrophages. Demonstrating a new paradigm, this proof of concept highlights the synergistic integration of live organisms and synthetic material engineering in a chairside treatment to address the multifaceted etiology of periodontitis.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging the versatile properties of bacterial spores in materials. 利用材料中细菌孢子的多功能特性。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-10-29 DOI: 10.1016/j.tibtech.2024.09.018

Likhitha Reddy Kummetha, Jeong-Joo Oh, Franka H van der Linden, Marie-Eve Aubin-Tam

引用次数: 0

A cell-free bacteriophage synthesis system for directed evolution. 用于定向进化的无细胞噬菌体合成系统。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-10-26 DOI: 10.1016/j.tibtech.2024.10.005

Bo Xu, Li-Hua Liu, Houliang Lin, Yang Zhang, Ying Huang, Qing He, Fan Wang, Yi-Rui Wu, Zhiqian Zhang, Ao Jiang

引用次数: 0

Emerging gene editing in industrial microbiology beyond CRISPR-Cas9. 超越 CRISPR-Cas9 的工业微生物学新兴基因编辑技术。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-10-26 DOI: 10.1016/j.tibtech.2024.09.012

Liya Liang, Huiping Tan, Rongming Liu

引用次数: 0

Fabricating an advanced electrogenic chassis by activating microbial metabolism and fine-tuning extracellular electron transfer. 通过激活微生物新陈代谢和微调细胞外电子传递，制造先进的电生底盘。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-10-25 DOI: 10.1016/j.tibtech.2024.09.021

Yang-Yang Fan, Qiang Tang, Yang Li, Hong Sun, Meiying Xu, Han-Qing Yu

{"title":"Fabricating an advanced electrogenic chassis by activating microbial metabolism and fine-tuning extracellular electron transfer.","authors":"Yang-Yang Fan, Qiang Tang, Yang Li, Hong Sun, Meiying Xu, Han-Qing Yu","doi":"10.1016/j.tibtech.2024.09.021","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.09.021","url":null,"abstract":"Exploiting electrogenic microorganisms as unconventional chassis hosts offers potential solutions to global energy and environmental challenges. However, their limited electrogenic efficiency and metabolic versatility, due to genetic and metabolic constraints, hinder broader applications. Herein, we developed a multifaceted approach to fabricate an enhanced electrogenic chassis, starting with streamlining the genome by removing extrachromosomal genetic material. This reduction led to faster lactate consumption, higher intracellular NADH/NAD+ and ATP/ADP levels, and increased growth and biomass accumulation, as well as promoted electrogenic activity. Transcriptome profiling showed an overall activation of cellular metabolism. We further established a molecular toolkit with a vector vehicle incorporating native replication block and refined promoter components for precise gene expression control. This enabled engineered primary metabolism for greater environmental robustness and fine-tuned extracellular electron transfer (EET) for improved efficiency. The enhanced chassis demonstrated substantially improved pollutant biodegradation and radionuclide removal, establishing a new paradigm for utilizing electrogenic organisms as novel biotechnology chassis.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro human cell-based models: What can they do and what are their limitations? 体外人体细胞模型：它们能做什么，有哪些局限性？

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-10-25 DOI: 10.1016/j.tibtech.2024.08.015

Matthias P Lutolf, Milica Radisic, Jeffrey Beekman, Dan Dongeun Huh, Meritxell Huch, Margherita Yayoi Turco, Zeinab Niloofar Tahmasebi Birgani, Dong Gao, Rui Yao, Hang Lin, Takanori Takebe

引用次数: 0

Cellobiose lipids: applications, production, and downstream processing. 纤维生物糖脂：应用、生产和下游加工。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-10-25 DOI: 10.1016/j.tibtech.2024.09.011

André D Valkenburg, Mellisa Z Ncube, George M Teke, Eugéne van Rensburg, Robert W M Pott

引用次数: 0

Comprehensive screening of industrially relevant components at genome scale using a high-quality gene overexpression collection of Corynebacterium glutamicum. 利用谷氨酸棒杆菌的高质量基因过表达集，在基因组规模上全面筛选工业相关成分。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-10-24 DOI: 10.1016/j.tibtech.2024.09.020

Jiao Liu, Xiaojia Zhao, Haijiao Cheng, Yanmei Guo, Xiaomeng Ni, Lixian Wang, Guannan Sun, Xiao Wen, Jiuzhou Chen, Jin Wang, Jingjing An, Xuan Guo, Zhenkun Shi, Haoran Li, Ruoyu Wang, Muqiang Zhao, Xiaoping Liao, Yu Wang, Ping Zheng, Meng Wang, Jibin Sun

{"title":"Comprehensive screening of industrially relevant components at genome scale using a high-quality gene overexpression collection of Corynebacterium glutamicum.","authors":"Jiao Liu, Xiaojia Zhao, Haijiao Cheng, Yanmei Guo, Xiaomeng Ni, Lixian Wang, Guannan Sun, Xiao Wen, Jiuzhou Chen, Jin Wang, Jingjing An, Xuan Guo, Zhenkun Shi, Haoran Li, Ruoyu Wang, Muqiang Zhao, Xiaoping Liao, Yu Wang, Ping Zheng, Meng Wang, Jibin Sun","doi":"10.1016/j.tibtech.2024.09.020","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.09.020","url":null,"abstract":"Development of efficient microbial strains for biomanufacturing requires deep understanding of the biology and functional components responsible for the synthesis, transport, and tolerance of the target compounds. A high-quality controllable gene overexpression strain collection was constructed for the industrial workhorse Corynebacterium glutamicum covering 99.7% of its genes. The collection was then used for comprehensive screening of components relevant to biomanufacturing features. In total, 15 components endowing cells with improved hyperosmotic tolerance and l-lysine productivity were identified, including novel transcriptional factors and DNA repair proteins. Systematic interrogation of a subset of the collection revealed efficient and specific exporters functioning in both C. glutamicum and Escherichia coli. Application of the new exporters was showcased to construct a strain with the highest l-threonine production level reported for C. glutamicum (75.1 g/l and 1.5 g/l·h) thus far. The genome-scale gene overexpression collection will serve as a valuable resource for fundamental biological studies and for developing industrial microorganisms for producing amino acids and other biochemicals.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0