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Engineering advanced in vitro models of endothelial dysfunction.
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-04 DOI: 10.1016/j.tibtech.2025.03.004
Jasneil Singh, Alexander M Ruhoff, Deepu Ashok, Steven G Wise, Anna Waterhouse
{"title":"Engineering advanced in vitro models of endothelial dysfunction.","authors":"Jasneil Singh, Alexander M Ruhoff, Deepu Ashok, Steven G Wise, Anna Waterhouse","doi":"10.1016/j.tibtech.2025.03.004","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.03.004","url":null,"abstract":"<p><p>Endothelial dysfunction is an important initiator of cardiovascular disease, the leading cause of death globally, and often manifests in arterial regions with disturbed blood flow. Experimental model advances have crucially helped unravel physiological mechanisms. While in vivo models provide a dynamic environment, they often fail to mimic human physiology precisely and face significant ethical barriers. Advanced in vitro models, including organs-on-chips and bioreactors, combine human cells and blood flow to accurately replicate endothelial dysfunction. Newer models have enhanced scalability and accuracy, with organs-on-chips commonly outperforming standard preclinical methods. Importantly, recent endothelial dysfunction discoveries leverage dynamic models to identify and evaluate clinically promising therapeutics. Here, we examine these developments and explore opportunities to develop next-generation in vitro models of endothelial dysfunction.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improving plant C-to-G base editors with a cold-adapted glycosylase and TadA-8e variants.
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-04 DOI: 10.1016/j.tibtech.2025.03.001
Yingli Jiang, Zhi Xiao, Zhaopeng Luo, Suhuai Zhou, Chaoyun Tong, Shan Jin, Xiaoshuang Liu, Ruiying Qin, Rongfang Xu, Lang Pan, Juan Li, Pengcheng Wei
{"title":"Improving plant C-to-G base editors with a cold-adapted glycosylase and TadA-8e variants.","authors":"Yingli Jiang, Zhi Xiao, Zhaopeng Luo, Suhuai Zhou, Chaoyun Tong, Shan Jin, Xiaoshuang Liu, Ruiying Qin, Rongfang Xu, Lang Pan, Juan Li, Pengcheng Wei","doi":"10.1016/j.tibtech.2025.03.001","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.03.001","url":null,"abstract":"<p><p>Plant cytosine (C)-to-guanine (G) base editors (CGBEs) have been established but suffer from limited editing efficiencies and low outcome purities. This study engineered a cod uracil DNA glycosylase (cod UNG, coUNG) from the cold-adapted fish Gadus morhua for plant CGBE, demonstrating 1.71- to 2.54-fold increases in C-to-G editing efficiency compared with the CGBE using human UNG (hUNG). Further engineering took advantage of TadA-8e-derived cytidine deaminases (TadA-CDs). These variants induced C substitutions with efficiencies ranging from 26.28% to 30.82% in rice cells, whereas adenine (A) conversion was negligible. By integrating coUNG and TadA-CDc elements with SpCas9 nickase, the resulting CDc-CGBEco achieved pure C-to-G editing without byproducts in up to 52.08% of transgenic lines. Whole-genome sequencing (WGS) analysis revealed no significant off-target effects of the CDc-BEs in rice. In addition, CDc-CGBEco enabled precise C-to-G editing in soybean and tobacco. These engineered CGBEs enhanced editing efficiency, purity, and specificity, suggesting their broad potential for applications in scientific research and crop breeding.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomically recoded organisms: redefining and safeguarding biological systems.
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-03 DOI: 10.1016/j.tibtech.2025.03.014
Miriam Amiram
{"title":"Genomically recoded organisms: redefining and safeguarding biological systems.","authors":"Miriam Amiram","doi":"10.1016/j.tibtech.2025.03.014","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.03.014","url":null,"abstract":"<p><p>In a recent report, Grome et al. describe a genomically recoded Escherichia coli strain with a 62-codon genome and a single stop codon. This and other genomically recoded organisms (GROs), engineered with modified genetic vocabularies, enable the creation of novel proteins and biomaterials, while ensuring the safety and viability of GRO-based biomanufacturing.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioprinting and synthetic biology approaches to engineer functional endocrine pancreatic constructs.
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-03 DOI: 10.1016/j.tibtech.2025.03.005
Davide Ribezzi, Pere Català, Cataldo Pignatelli, Antonio Citro, Riccardo Levato
{"title":"Bioprinting and synthetic biology approaches to engineer functional endocrine pancreatic constructs.","authors":"Davide Ribezzi, Pere Català, Cataldo Pignatelli, Antonio Citro, Riccardo Levato","doi":"10.1016/j.tibtech.2025.03.005","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.03.005","url":null,"abstract":"<p><p>Diabetes is a complex disease affecting over 500 million people worldwide. Traditional approaches, such as insulin delivery, are mainstay treatments, but do not cure the disease. Recent advances in biofabrication and synthetic biology offer new hope for the development of tissue constructs recapitulating salient organ functions. Here, we discuss recent progress in bioprinting a functional endocrine pancreas, ranging from cell sources to main advances in biomaterials. We review innovative areas for the development of this field, with a particular focus on the convergence of synthetic biology and cell engineering with bioprinting, which opens new avenues for developing advanced in vitro models and regenerative, transplantable grafts, with the potential to provide independence from exogenous insulin administration.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions. 可编程嵌入式生物打印一步制造动脉模型与定制的收缩和代谢功能。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2025-01-07 DOI: 10.1016/j.tibtech.2024.11.019
Qi Li, Shuyuan Yu, Yuxuan Wang, Hui Zhao, Ziqi Gao, Huilong Du, Huayong Yang, Luqi Shen, Hongzhao Zhou
{"title":"Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions.","authors":"Qi Li, Shuyuan Yu, Yuxuan Wang, Hui Zhao, Ziqi Gao, Huilong Du, Huayong Yang, Luqi Shen, Hongzhao Zhou","doi":"10.1016/j.tibtech.2024.11.019","DOIUrl":"10.1016/j.tibtech.2024.11.019","url":null,"abstract":"<p><p>Replicating the contractile function of arterial tissues in vitro requires precise control of cell alignment within 3D structures, a challenge that existing bioprinting techniques struggle to meet. In this study, we introduce the voxel-based embedded construction for tailored orientational replication (VECTOR) method, a voxel-based approach that controls cellular orientation and collective behavior within bioprinted filaments. By fine-tuning voxel vector magnitude and using an omnidirectional printing trajectory, we achieve structural mimicry at both the macroscale and the cellular alignment level. This dual-scale approach enhances vascular smooth muscle cell (VSMC) function by regulating contractile and synthetic pathways. The VECTOR method facilitates the construction of 3D arterial structures that closely replicate natural coronary architectures, significantly improving contractility and metabolic function. Moreover, the resulting multilayered arterial models (AMs) exhibit precise responses to pharmacological stimuli, similar to native arteries. This work highlights the critical role of structural mimicry in tissue functionality and advances the replication of complex tissues in vitro.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"918-945"},"PeriodicalIF":14.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Programmable ultrasound-mediated swarms manipulation of bacteria-red blood cell microrobots for tumor-specific thrombosis and robust photothermal therapy. 可编程超声介导的细菌-红细胞微机器人群体操作，用于肿瘤特异性血栓形成和强大的光热治疗。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2024-12-20 DOI: 10.1016/j.tibtech.2024.11.018
Hui Ran, Ye Yang, Weijing Han, Ruijing Liang, Denghui Zhu, Bing Yuan, Cheng Xu, Dan Li, Jian Ren, Hong Pan, Lanlan Liu, Teng Ma, Aiqing Ma, Lintao Cai
{"title":"Programmable ultrasound-mediated swarms manipulation of bacteria-red blood cell microrobots for tumor-specific thrombosis and robust photothermal therapy.","authors":"Hui Ran, Ye Yang, Weijing Han, Ruijing Liang, Denghui Zhu, Bing Yuan, Cheng Xu, Dan Li, Jian Ren, Hong Pan, Lanlan Liu, Teng Ma, Aiqing Ma, Lintao Cai","doi":"10.1016/j.tibtech.2024.11.018","DOIUrl":"10.1016/j.tibtech.2024.11.018","url":null,"abstract":"<p><p>Despite the excellent advantages of biomicrorobots, such as autonomous navigation and targeting actuation, effective penetration and retention to deep lesion sites for effective therapy remains a longstanding challenge. Here, we present dual-engine cell microrobots, which we refer to as PR-robots, created by conjugating photosynthetic bacteria (PSB) with red blood cells (RBCs). The robots penetrate the tumor interior in swarms through combined hypoxic traction and ultrasound actuation (UA). The hypoxia-targeting ability of PSB induced PR-robot accumulation in the tumor region. Subsequently, programmable UA trapped the PR-robots to form bioswarms and traverse tissue obstacles, penetrating the tumor interior. The substantial influx of PR-robots into the tumor tissue promoted the formation of tumor-specific thrombus (TST). Finally, the PSB and TST synergistically improved the effect of photothermal therapy. Thus, these advantages of remote ultrasound control technology pave the way for various new therapies in practical biomedicine.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"868-892"},"PeriodicalIF":14.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oil-water interfaces of Pickering emulsions: microhabitats for living cell biocatalysis. 皮克林乳液的油水界面：活细胞生物催化的微生境。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2024-10-11 DOI: 10.1016/j.tibtech.2024.09.014
Daniel Chikere Ali, Tao Pan, Qingping Wu, Zhilong Wang
{"title":"Oil-water interfaces of Pickering emulsions: microhabitats for living cell biocatalysis.","authors":"Daniel Chikere Ali, Tao Pan, Qingping Wu, Zhilong Wang","doi":"10.1016/j.tibtech.2024.09.014","DOIUrl":"10.1016/j.tibtech.2024.09.014","url":null,"abstract":"<p><p>Based on the size of bacterial cells and bacterial surface hydrophobicity, some bacteria meet the requirements of Pickering particles to stabilize Pickering emulsions. Here, we discuss the oil-water interfaces of bacteria-stabilized Pickering emulsions as microhabitats for microbial metabolism of oil-soluble chemicals. The correlation between living bacteria-stabilized Pickering emulsions and microhabitats of living bacteria at oil-water interfaces offers a new perspective to study bioprocess engineering at the mesoscale between the cell and reactor scales, which not only provides novel parameters to optimize the bioprocess engineering, but also unravels the paradox of some natural phenomena related to living cell biocatalysis.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"790-801"},"PeriodicalIF":14.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant-derived biomass-based hydrogels for biomedical applications. 用于生物医学应用的植物生物质水凝胶。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2024-10-08 DOI: 10.1016/j.tibtech.2024.09.010
Tushuai Li, Wenxue Sun, Da Qian, Peng Wang, Xingyu Liu, Chengsheng He, Tong Chang, Guangfu Liao, Jie Zhang
{"title":"Plant-derived biomass-based hydrogels for biomedical applications.","authors":"Tushuai Li, Wenxue Sun, Da Qian, Peng Wang, Xingyu Liu, Chengsheng He, Tong Chang, Guangfu Liao, Jie Zhang","doi":"10.1016/j.tibtech.2024.09.010","DOIUrl":"10.1016/j.tibtech.2024.09.010","url":null,"abstract":"<p><p>Hydrogels made of plant-derived biomass have gained popularity in biomedical applications because they are frequently affordable, readily available, and biocompatible. Finding the perfect plant-derived biomass-based hydrogels for biomedicine that can replicate essential characteristics of human tissues in regard to structure, function, and performance has proved to be difficult. In this review, we summarize some of the major contributions made to this topic, covering basic ideas and different biomass-based hydrogels made of cellulose, hemicellulose, and lignin. Also included is an in-depth discussion regarding the biosafety and toxicity assessments of biomass-based hydrogels. Finally, this review also highlights important scientific debates and major obstacles regarding biomass-based hydrogels for biomedical applications.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"802-811"},"PeriodicalIF":14.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Leveraging the versatile properties of bacterial spores in materials. 利用材料中细菌孢子的多功能特性。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2024-10-29 DOI: 10.1016/j.tibtech.2024.09.018
Likhitha Reddy Kummetha, Jeong-Joo Oh, Franka H van der Linden, Marie-Eve Aubin-Tam
{"title":"Leveraging the versatile properties of bacterial spores in materials.","authors":"Likhitha Reddy Kummetha, Jeong-Joo Oh, Franka H van der Linden, Marie-Eve Aubin-Tam","doi":"10.1016/j.tibtech.2024.09.018","DOIUrl":"10.1016/j.tibtech.2024.09.018","url":null,"abstract":"<p><p>Inspired by biological functions of living systems, researchers have engineered cells as independent functional materials or integrated them within a natural or synthetic matrix to create engineered living materials (ELMs). However, the 'livingness' of cells in such materials poses serious drawbacks, such as a short lifespan and the need for cold-chain logistics. Bacterial spores have emerged as a game changer to bypass these shortcomings as a result of their intrinsic dormancy and resistance against harsh conditions. Emerging synthetic biology tools tailored for engineering spores and better understanding of their physical properties have led to novel applications of spore-based materials. Here, we review recent advances in such materials and discuss future challenges for the development of time- and cost-efficient spore-based materials with high performance.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"812-825"},"PeriodicalIF":14.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid manufacturing of CAR-T therapy: strategies and impact. CAR-T 疗法的快速制造：战略与影响。
IF 14.3 1区 工程技术
Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2024-10-09 DOI: 10.1016/j.tibtech.2024.09.013
Sixun Chen, Dan Liu
{"title":"Rapid manufacturing of CAR-T therapy: strategies and impact.","authors":"Sixun Chen, Dan Liu","doi":"10.1016/j.tibtech.2024.09.013","DOIUrl":"10.1016/j.tibtech.2024.09.013","url":null,"abstract":"<p><p>The accessibility of autologous chimeric antigen receptor T cell (CAR-T) therapies is challenged by the complex processes and capacity constraints of manufacturing. Rapid manufacturing capable of shortening manufacturing timelines could transform the CAR-T field. Here, we outline approaches to rapid CAR-T manufacturing, highlighting its impact on various stakeholders in the landscape.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"745-748"},"PeriodicalIF":14.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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