Trends in biotechnology最新文献

Emerging supramolecular and living materials in oral medicine. 口腔医学中新兴的超分子和活性材料。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-07 DOI: 10.1016/j.tibtech.2025.03.006

Nicholas G Fischer, Tsung-Yi Lin, Yuanhui Xiang, Ting Sang, Zhou Ye

引用次数: 0

Advancing wheat breeding using rye: a key contribution to wheat breeding history. 利用黑麦推进小麦育种：对小麦育种史的重大贡献。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-07 DOI: 10.1016/j.tibtech.2025.03.008

Guohao Han, Hanwen Yan, Lihui Li, Diaoguo An

引用次数: 0

Long-offset paired nicking-based efficient and precise strategy for in vivo targeted insertion. 基于长偏移配对缺口的高效精准体内靶向插入策略。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-07 DOI: 10.1016/j.tibtech.2025.02.020

Yafang Lu, Jialu Wang, Yilun Xu, Mengli Xu, Borui Li, Zhan Fan, Jinxin Liu, Xinlin Li, Zhenzhen Cai, Yuanzhe Zheng, Wenjing Wang, Jie Yang, Zhihong Zhang, Zheng Liu

{"title":"Long-offset paired nicking-based efficient and precise strategy for in vivo targeted insertion.","authors":"Yafang Lu, Jialu Wang, Yilun Xu, Mengli Xu, Borui Li, Zhan Fan, Jinxin Liu, Xinlin Li, Zhenzhen Cai, Yuanzhe Zheng, Wenjing Wang, Jie Yang, Zhihong Zhang, Zheng Liu","doi":"10.1016/j.tibtech.2025.02.020","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.02.020","url":null,"abstract":"Clustered regularly interspaced short palindromic repeat (CRISPR)-based targeted insertion of DNA fragments holds great promise for gene therapy. However, designing highly efficient and precise integration of large DNA segments in somatic cells while avoiding unpredictable products remains challenging. Here, we devised a novel long-offset paired nicking target integration (LOTI) strategy, which enhances the capacity of Cas9 nickase (Cas9n) in targeted gene integration in somatic cells, yielding higher knock-in (KI) efficiency compared with classical nickase-based approaches. The underlying repair mechanism involves the DNA repair proteins Rad51 and Rad52, and Ligase I/III. Moreover, we achieved efficient KI of at least 1.5-kb gene fragments in hepatocytes and recovery 55% FIX activity in a hemophilia B mouse model using only one-dose plasmid DNA delivery. Compared with the Cas9-based strategy, LOTI reduces off-target activity and restricts the formulation of unwanted insertions and deletions (indels) at the target site. Thus, LOTI provides a precise and efficient strategy for gene integration in somatic cells in vivo.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering advanced in vitro models of endothelial dysfunction. 内皮功能障碍的体外模型的工程进展。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-04 DOI: 10.1016/j.tibtech.2025.03.004

Jasneil Singh, Alexander M Ruhoff, Deepu Ashok, Steven G Wise, Anna Waterhouse

引用次数: 0

Improving plant C-to-G base editors with a cold-adapted glycosylase and TadA-8e variants. 利用冷适应糖基酶和TadA-8e变体改进植物C-to-G碱基编辑器。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-04 DOI: 10.1016/j.tibtech.2025.03.001

Yingli Jiang, Zhi Xiao, Zhaopeng Luo, Suhuai Zhou, Chaoyun Tong, Shan Jin, Xiaoshuang Liu, Ruiying Qin, Rongfang Xu, Lang Pan, Juan Li, Pengcheng Wei

{"title":"Improving plant C-to-G base editors with a cold-adapted glycosylase and TadA-8e variants.","authors":"Yingli Jiang, Zhi Xiao, Zhaopeng Luo, Suhuai Zhou, Chaoyun Tong, Shan Jin, Xiaoshuang Liu, Ruiying Qin, Rongfang Xu, Lang Pan, Juan Li, Pengcheng Wei","doi":"10.1016/j.tibtech.2025.03.001","DOIUrl":"https://doi.org/10.1016/j.tibtech.2025.03.001","url":null,"abstract":"Plant cytosine (C)-to-guanine (G) base editors (CGBEs) have been established but suffer from limited editing efficiencies and low outcome purities. This study engineered a cod uracil DNA glycosylase (cod UNG, coUNG) from the cold-adapted fish Gadus morhua for plant CGBE, demonstrating 1.71- to 2.54-fold increases in C-to-G editing efficiency compared with the CGBE using human UNG (hUNG). Further engineering took advantage of TadA-8e-derived cytidine deaminases (TadA-CDs). These variants induced C substitutions with efficiencies ranging from 26.28% to 30.82% in rice cells, whereas adenine (A) conversion was negligible. By integrating coUNG and TadA-CDc elements with SpCas9 nickase, the resulting CDc-CGBEco achieved pure C-to-G editing without byproducts in up to 52.08% of transgenic lines. Whole-genome sequencing (WGS) analysis revealed no significant off-target effects of the CDc-BEs in rice. In addition, CDc-CGBEco enabled precise C-to-G editing in soybean and tobacco. These engineered CGBEs enhanced editing efficiency, purity, and specificity, suggesting their broad potential for applications in scientific research and crop breeding.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomically recoded organisms: redefining and safeguarding biological systems. 基因组编码生物体：重新定义和保护生物系统。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-03 DOI: 10.1016/j.tibtech.2025.03.014

Miriam Amiram

引用次数: 0

Bioprinting and synthetic biology approaches to engineer functional endocrine pancreatic constructs. 生物打印和合成生物学方法工程功能内分泌胰腺结构。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-03 DOI: 10.1016/j.tibtech.2025.03.005

Davide Ribezzi, Pere Català, Cataldo Pignatelli, Antonio Citro, Riccardo Levato

引用次数: 0

Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions. 可编程嵌入式生物打印一步制造动脉模型与定制的收缩和代谢功能。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2025-01-07 DOI: 10.1016/j.tibtech.2024.11.019

Qi Li, Shuyuan Yu, Yuxuan Wang, Hui Zhao, Ziqi Gao, Huilong Du, Huayong Yang, Luqi Shen, Hongzhao Zhou

{"title":"Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions.","authors":"Qi Li, Shuyuan Yu, Yuxuan Wang, Hui Zhao, Ziqi Gao, Huilong Du, Huayong Yang, Luqi Shen, Hongzhao Zhou","doi":"10.1016/j.tibtech.2024.11.019","DOIUrl":"10.1016/j.tibtech.2024.11.019","url":null,"abstract":"Replicating the contractile function of arterial tissues in vitro requires precise control of cell alignment within 3D structures, a challenge that existing bioprinting techniques struggle to meet. In this study, we introduce the voxel-based embedded construction for tailored orientational replication (VECTOR) method, a voxel-based approach that controls cellular orientation and collective behavior within bioprinted filaments. By fine-tuning voxel vector magnitude and using an omnidirectional printing trajectory, we achieve structural mimicry at both the macroscale and the cellular alignment level. This dual-scale approach enhances vascular smooth muscle cell (VSMC) function by regulating contractile and synthetic pathways. The VECTOR method facilitates the construction of 3D arterial structures that closely replicate natural coronary architectures, significantly improving contractility and metabolic function. Moreover, the resulting multilayered arterial models (AMs) exhibit precise responses to pharmacological stimuli, similar to native arteries. This work highlights the critical role of structural mimicry in tissue functionality and advances the replication of complex tissues in vitro.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"918-945"},"PeriodicalIF":14.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Programmable ultrasound-mediated swarms manipulation of bacteria-red blood cell microrobots for tumor-specific thrombosis and robust photothermal therapy. 可编程超声介导的细菌-红细胞微机器人群体操作，用于肿瘤特异性血栓形成和强大的光热治疗。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2024-12-20 DOI: 10.1016/j.tibtech.2024.11.018

Hui Ran, Ye Yang, Weijing Han, Ruijing Liang, Denghui Zhu, Bing Yuan, Cheng Xu, Dan Li, Jian Ren, Hong Pan, Lanlan Liu, Teng Ma, Aiqing Ma, Lintao Cai

{"title":"Programmable ultrasound-mediated swarms manipulation of bacteria-red blood cell microrobots for tumor-specific thrombosis and robust photothermal therapy.","authors":"Hui Ran, Ye Yang, Weijing Han, Ruijing Liang, Denghui Zhu, Bing Yuan, Cheng Xu, Dan Li, Jian Ren, Hong Pan, Lanlan Liu, Teng Ma, Aiqing Ma, Lintao Cai","doi":"10.1016/j.tibtech.2024.11.018","DOIUrl":"10.1016/j.tibtech.2024.11.018","url":null,"abstract":"Despite the excellent advantages of biomicrorobots, such as autonomous navigation and targeting actuation, effective penetration and retention to deep lesion sites for effective therapy remains a longstanding challenge. Here, we present dual-engine cell microrobots, which we refer to as PR-robots, created by conjugating photosynthetic bacteria (PSB) with red blood cells (RBCs). The robots penetrate the tumor interior in swarms through combined hypoxic traction and ultrasound actuation (UA). The hypoxia-targeting ability of PSB induced PR-robot accumulation in the tumor region. Subsequently, programmable UA trapped the PR-robots to form bioswarms and traverse tissue obstacles, penetrating the tumor interior. The substantial influx of PR-robots into the tumor tissue promoted the formation of tumor-specific thrombus (TST). Finally, the PSB and TST synergistically improved the effect of photothermal therapy. Thus, these advantages of remote ultrasound control technology pave the way for various new therapies in practical biomedicine.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"868-892"},"PeriodicalIF":14.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oil-water interfaces of Pickering emulsions: microhabitats for living cell biocatalysis. 皮克林乳液的油水界面：活细胞生物催化的微生境。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2024-10-11 DOI: 10.1016/j.tibtech.2024.09.014

Daniel Chikere Ali, Tao Pan, Qingping Wu, Zhilong Wang

引用次数: 0