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Sensing the future of cell therapy manufacturing. 感知细胞治疗制造的未来。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-03 DOI: 10.1016/j.tibtech.2026.08.010
Masoud Khazaei, Muamer Dervisevic, Nicolas H Voelcker
{"title":"Sensing the future of cell therapy manufacturing.","authors":"Masoud Khazaei, Muamer Dervisevic, Nicolas H Voelcker","doi":"10.1016/j.tibtech.2026.08.010","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.010","url":null,"abstract":"<p><p>Cell therapies are increasingly manufactured in closed, automated culture systems, yet decisions about cell quality, safety, and release still rely largely on sparsely executed, destructive, and offline assays. Over the past 5 years, significant progress has been made in developing biosensors capable of real-time measurement of key biochemical and physicochemical indicators including metabolites, ionic species, and protein-associated stress indicators within cell culture environments. However, most systems remain disconnected from manufacturing workflows and fail to support actionable process control. This opinion article discusses the next phase of innovation and the need to reposition biosensors as process analytical technology, shifting the field from descriptive monitoring toward integrated, decision-driven control of cell therapy manufacturing.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beyond statutory compliance: toward comprehensive genomic governance. 超越法规遵从：迈向全面的基因组治理。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-03 DOI: 10.1016/j.tibtech.2026.08.009
Guillermo Aquino-Jarquin
{"title":"Beyond statutory compliance: toward comprehensive genomic governance.","authors":"Guillermo Aquino-Jarquin","doi":"10.1016/j.tibtech.2026.08.009","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.009","url":null,"abstract":"<p><p>I welcome the discussion raised by Hernández-Huerta and colleagues. While legal provisions regulating the transfer of biological materials are significant, genomic governance goes beyond mere legal compliance. It encompasses transparency, accountability, benefit-sharing, and long-term stewardship. Recent international recommendations emphasize the need for comprehensive governance frameworks for national genomic initiatives.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advancing European standards and regulations for 3D bioprinting. 推进欧洲3D生物打印标准和法规。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-03 DOI: 10.1016/j.tibtech.2026.08.001
Ambra Maddalon, Giulia Leonetti, Sophie Zimmer
{"title":"Advancing European standards and regulations for 3D bioprinting.","authors":"Ambra Maddalon, Giulia Leonetti, Sophie Zimmer","doi":"10.1016/j.tibtech.2026.08.001","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.001","url":null,"abstract":"<p><p>3D bioprinting has the potential to provide nonanimal alternatives to preclinical testing and revolutionize current clinical and transplantation practices. Realizing this potential requires a concerted effort to develop and advance European standards and regulatory frameworks, ensuring a clear and safe pathway for the clinical integration of 3D bioprinting.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biotechnology-driven artificial diets for mass-rearing arthropod natural enemies. 大规模饲养节肢动物天敌的生物技术驱动人工饲料。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-01-22 DOI: 10.1016/j.tibtech.2025.12.010
Yong Wang, Wei-Cheng Yang, Patrick De Clercq, Norman C Leppla, Adeney de Freitas Bueno, Ricardo Ramirez-Romero, Nicolas Desneux, Lian-Sheng Zang
{"title":"Biotechnology-driven artificial diets for mass-rearing arthropod natural enemies.","authors":"Yong Wang, Wei-Cheng Yang, Patrick De Clercq, Norman C Leppla, Adeney de Freitas Bueno, Ricardo Ramirez-Romero, Nicolas Desneux, Lian-Sheng Zang","doi":"10.1016/j.tibtech.2025.12.010","DOIUrl":"10.1016/j.tibtech.2025.12.010","url":null,"abstract":"<p><p>Arthropod natural enemies are central to biological control programs, where they regulate pest populations while contributing to ecological stability and biodiversity conservation. Nonetheless, for many species, large-scale rearing of these arthropods is constrained by expensive, labor-intensive methods that still rely heavily on living hosts. Emerging biotechnological tools promise to transform rearing practices by supporting the design of accurate, affordable, and host-independent artificial diets for arthropod natural enemies. This review explores biotechnology-driven advances in nutrient profiling, low-cost production, and functional packaging and integrates them into a unified framework. Moreover, this review highlights how the integration of multidisciplinary approaches and biotechnological innovations can address critical challenges in artificial diet development to enable sustainable biocontrol pest management at practical scales.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2530-2544"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146041762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multifunctional biosensors redefining liquid biopsy: advancing simultaneous multianalyte detection. 多功能生物传感器重新定义液体活检：推进同步多分析物检测。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-02-14 DOI: 10.1016/j.tibtech.2025.12.026
Panagiota M Kalligosfyri, Antonella Miglione, Ada Raucci, Wanda Cimmino, Gabriella Iula, Alessandra Glovi, Sima Singh, Michelino De Laurentiis, Antonio Giordano, Stefano Cinti
{"title":"Multifunctional biosensors redefining liquid biopsy: advancing simultaneous multianalyte detection.","authors":"Panagiota M Kalligosfyri, Antonella Miglione, Ada Raucci, Wanda Cimmino, Gabriella Iula, Alessandra Glovi, Sima Singh, Michelino De Laurentiis, Antonio Giordano, Stefano Cinti","doi":"10.1016/j.tibtech.2025.12.026","DOIUrl":"10.1016/j.tibtech.2025.12.026","url":null,"abstract":"<p><p>Liquid biopsy has revolutionized noninvasive disease diagnosis and monitoring by detecting circulating biomarkers such as tumor-derived DNA, miRNAs, proteins, exosomes, and circulating tumor cells in body fluids. Conventional single-analyte approaches fail to capture disease complexity, limiting diagnostic accuracy. Multifunctional biosensors enable simultaneous detection of multiple biomarkers, representing a paradigm shift in liquid biopsy. This review highlights recent advances in diverse detection strategies, including optical, electrochemical, plasmonic, and clustered regularly interspaced short palindromic repeats-based assays, alongside innovations in hybrid platforms, smart surface engineering, and nanotechnology. We also discuss integration with microfluidics, artificial intelligence, and chemometric analysis to enhance sensitivity, reduce signal interference, and improve scalability. Finally, challenges in clinical translation and standardization are highlighted, underscoring the transformative potential of multifunctional biosensors in precision medicine and early disease detection.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2577-2595"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146198116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thermotolerant yeasts and their biotechnological applications. 耐热酵母及其生物技术应用。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-02-23 DOI: 10.1016/j.tibtech.2025.12.025
Roksolana Vasylyshyn, Justyna Ruchala, Kostyantyn Dmytruk, Andriy Sibirny
{"title":"Thermotolerant yeasts and their biotechnological applications.","authors":"Roksolana Vasylyshyn, Justyna Ruchala, Kostyantyn Dmytruk, Andriy Sibirny","doi":"10.1016/j.tibtech.2025.12.025","DOIUrl":"10.1016/j.tibtech.2025.12.025","url":null,"abstract":"<p><p>Most known yeasts are mesophilic organisms, with optimal growth at 28-30°C. However, a few species, notably Ogataea polymorpha and Kluyveromyces marxianus, can grow at temperatures around 50°C. Their thermotolerance is of both fundamental and applied interest, offering advantages for high-temperature bioprocesses. Notably, cultivation at elevated temperatures facilitates simultaneous saccharification and fermentation (SSF) of starchy and lignocellulosic substrates, reduces sterility requirements, lowers distillation costs, and enhances the rates of biochemical reactions. These features make thermotolerant yeasts attractive platforms for producing biofuels, proteins, and other valuable compounds. Despite their potential, the underlying mechanisms of thermotolerance in these species remain underexplored. Herein, we systematically review the molecular, cellular, and metabolic mechanisms underlying yeast thermotolerance and discuss their implications for high-temperature industrial biotechnology.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2563-2576"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recruiting orthogonal biological systems for engineering microorganisms. 为工程微生物招募正交生物系统。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-03-05 DOI: 10.1016/j.tibtech.2025.12.023
Kaifang Liu, Zhilan Zhang, Guangjie Liang, Cong Gao, Guipeng Hu, Haiqin Chen, Liming Liu, Jing Wu
{"title":"Recruiting orthogonal biological systems for engineering microorganisms.","authors":"Kaifang Liu, Zhilan Zhang, Guangjie Liang, Cong Gao, Guipeng Hu, Haiqin Chen, Liming Liu, Jing Wu","doi":"10.1016/j.tibtech.2025.12.023","DOIUrl":"10.1016/j.tibtech.2025.12.023","url":null,"abstract":"<p><p>Microbial synthetic biology focuses on the application of rational engineering strategies to reprogram microbial cells, thereby providing them with novel functions to meet different requirements. However, this engineering process inherently disrupts natural metabolism, leading to increased complexity and unpredictability within the metabolic system. To address these challenges, a series of orthogonal strategies has been developed and implemented in the construction of orthogonal genetic systems, metabolic pathways, energy systems, and regulatory systems. This review summarizes recent advances and applications of orthogonal strategies in microbial synthetic biology. Finally, future research directions in orthogonal microbial synthetic biology are explored, aiming to provide new insights for future studies.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2629-2645"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147366608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrostatic remodeling of TadA8e* eliminates ABE8e genome-wide off-target effects. TadA8e*的静电重塑消除了ABE8e全基因组的脱靶效应。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-04-10 DOI: 10.1016/j.tibtech.2026.03.019
Long Xie, Mengxue Ma, Baishui He, Danrong Jiao, Erwei Zuo
{"title":"Electrostatic remodeling of TadA8e* eliminates ABE8e genome-wide off-target effects.","authors":"Long Xie, Mengxue Ma, Baishui He, Danrong Jiao, Erwei Zuo","doi":"10.1016/j.tibtech.2026.03.019","DOIUrl":"10.1016/j.tibtech.2026.03.019","url":null,"abstract":"<p><p>Although gene editing offers durable therapeutic potential for genetic disorders such as hypercholesterolemia, widely used adenine base editors (ABEs) face translational obstacles due to potential off-target effects inherent in gene-editing systems. ABE8e has strong clinical potential owing to its high efficiency and rapid editing kinetics, but its genome-wide off-target effects remain poorly characterized. Here, we uncovered substantial genome-wide off-target edits of ABE8e using the GOTI (Genome-wide Off-target analysis by Two-cell embryo Injection) assay, including in oncogenes and tumor suppressors, highlighting significant risks for clinical translation. We therefore engineered a next-generation precision base editor, erABE, through electrostatic remodeling of the TadA8e deaminase. erABE reduces ABE8e's genome-wide off-target activity by ~36.5-fold to background levels while retaining an efficiency indistinguishable from ABE8e. In mice, a single low-dose delivery of erABE packaged in lipid nanoparticles achieved robust Pcsk9 knockdown, resulting in a sustained, ~90% reduction in plasma PCSK9 protein and ~60% reduction in low-density lipoprotein cholesterol for at least 6 months. erABE also potently suppresses PCSK9 expression in human liver organoids. These results highlight the safety and durable therapeutic effects of erABE, supporting its translation as a clinical genome-editing platform.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2722-2743"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147677038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
When gas-grown biomass becomes a food material. 当气体生长的生物质成为一种食物材料。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-08-03 DOI: 10.1016/j.tibtech.2026.07.024
Peter Battle, Ying Chen
{"title":"When gas-grown biomass becomes a food material.","authors":"Peter Battle, Ying Chen","doi":"10.1016/j.tibtech.2026.07.024","DOIUrl":"10.1016/j.tibtech.2026.07.024","url":null,"abstract":"<p><p>Hydrogen fermentation can generate protein-rich microbial biomass without agricultural carbon feedstocks, but cultivation alone does not create a food ingredient. Woern and colleagues establish a targeted route for protein recovery from Cupriavidus necator. Their study positions downstream processing as a bridge from gas-grown cells toward functional, safe, and scalable food ingredients.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2498-2500"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148670605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epigenetic editing for next-generation climate-smart crops. 下一代气候智能型作物的表观遗传编辑。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-09-01 DOI: 10.1016/j.tibtech.2026.08.006
Xianyun Yu, Jisen Zhang, Qiutao Xu
{"title":"Epigenetic editing for next-generation climate-smart crops.","authors":"Xianyun Yu, Jisen Zhang, Qiutao Xu","doi":"10.1016/j.tibtech.2026.08.006","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.08.006","url":null,"abstract":"<p><p>Epigenetic editing enables programmable and potentially reversible regulation of chromatin states without altering DNA sequences. By establishing a framework of programmable chromatin engineering, epigenetic editing provides new opportunities for designing climate-resilient crops and advancing next-generation precision breeding.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148876046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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