Trends in biotechnology最新文献_第2页

Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow. 连接器支持金门组装和酵母重组的集成，以简化生物铸造工作流程中的多基因途径构建。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2026-01-14 DOI: 10.1016/j.tibtech.2025.12.004

Min-Jun Seong, Haneul Kim, Hyewon Lee, Haseong Kim, Seung-Goo Lee, Min Ju Lee, Ji-Su Jun, Sujin Hong, Tae Hyun Kim, Seong Keun Kim, Jonghyeok Shin, Dae-Hee Lee

{"title":"Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow.","authors":"Min-Jun Seong, Haneul Kim, Hyewon Lee, Haseong Kim, Seung-Goo Lee, Min Ju Lee, Ji-Su Jun, Sujin Hong, Tae Hyun Kim, Seong Keun Kim, Jonghyeok Shin, Dae-Hee Lee","doi":"10.1016/j.tibtech.2025.12.004","DOIUrl":"10.1016/j.tibtech.2025.12.004","url":null,"abstract":"Rewiring the metabolic flux for efficient microbial conversion requires robust, scalable gene assembly. However, conventional gene assembly approaches are labor-intensive, highly experience-dependent, and require extensive expertise to ensure reproducibility and efficiency. Even with advanced automation platforms such as biofoundries, assembling gene arrays with multiple transcriptional units (TUs) remains challenging. In this study, we present Efficient Modular Gene Assembly (EffiModular), an integrated in vitro and in vivo gene assembly platform compatible with automated workflows. EffiModular enables the assembly of up to eight TUs with 80% efficiency in a single transformation. Integrated into a biofoundry workflow, it enabled the construction of 120 distinct yeast strains with varying levels of expression of the β-carotene biosynthesis genes within 3 days. Compared with conventional approaches, it significantly reduces procedural complexity, minimizes reliance on operator expertise, and accelerates workflow timelines. These features establish EffiModular as a next-generation gene assembly platform for scalable, reproducible gene assembly in biofoundry-based genetic engineering.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"1478-1497"},"PeriodicalIF":14.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145985147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae-made biopharmaceuticals and their potential role in the One Health approach. 微藻制造的生物制药及其在“同一个健康”方案中的潜在作用。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2025-09-25 DOI: 10.1016/j.tibtech.2025.08.018

Ileana García-Silva, Sornkanok Vimolmangkang, Sergio Rosales-Mendoza

引用次数: 0

A practical guide to spatial transcriptomics: lessons from over 1000 samples. 空间转录组学实用指南：来自1000多个样本的经验教训。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2025-09-19 DOI: 10.1016/j.tibtech.2025.08.020

Daniela Grases, Eduard Porta-Pardo

引用次数: 0

Engineered coatings containing cyclic peptides from cyanobacteria delay the development of a stable macrofouling community. 含有来自蓝藻的环肽的工程涂层延缓了稳定的大污染群落的发展。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2026-01-13 DOI: 10.1016/j.tibtech.2025.11.018

Catarina Gonçalves, Sandra Pereira, Isabel B Oliveira, Marco Preto, Tiago Ribeiro, João Morais, Luciana C Gomes, Maria João Romeu, Miguel Semedo, Filipe J Mergulhão, Vitor Vasconcelos, Elisabete R Silva, Joana R Almeida

{"title":"Engineered coatings containing cyclic peptides from cyanobacteria delay the development of a stable macrofouling community.","authors":"Catarina Gonçalves, Sandra Pereira, Isabel B Oliveira, Marco Preto, Tiago Ribeiro, João Morais, Luciana C Gomes, Maria João Romeu, Miguel Semedo, Filipe J Mergulhão, Vitor Vasconcelos, Elisabete R Silva, Joana R Almeida","doi":"10.1016/j.tibtech.2025.11.018","DOIUrl":"10.1016/j.tibtech.2025.11.018","url":null,"abstract":"Biofouling-the adhesion of organisms and their byproducts to submerged surfaces-poses economic and environmental challenges, highlighting the need for sustainable antifouling solutions. This study reports a proof-of-concept investigation into the environmental compatibility and field validation of natural cyclic peptides portoamides A and B (Pam) as a bio-based antifouling alternative. Pam have demonstrated antifouling activity by inhibiting mussel larval settlement and disrupting biofilm formation. Herein, the antifouling performance of Pam-engineered coatings was evaluated through anti-settlement, anti-biofilm, as well as marine field tests. Lab-scale tests revealed that Pam-based coatings (0.7 wt%) effectively reduced biofilm thickness, surface coverage, and mussel larval settlement. Field trials showed that Pam-functionalized coating prototypes outperformed a commercial biocide in use (Econea®), delaying macrofouling community establishment and contributing to enhanced antifouling effectiveness. Overall, this work supports further development of antifouling engineered systems using Pam, representing a significant technological advance (from Technology Readiness Level 3 to 6) toward sustainable marine coating systems.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"1330-1353"},"PeriodicalIF":14.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145985249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pipeline and insulated dual-reporter system to predict and quantify strong terminators from RNA-seq datasets for metabolic engineering. 管道和绝缘双报告系统，用于预测和量化代谢工程RNA-seq数据集的强终止子。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2026-01-02 DOI: 10.1016/j.tibtech.2025.11.017

Yunhao Chen, Qiaoning He, Qiushi Ye, Jiapeng Li, Changzheng Li, Shihui Yang

{"title":"Pipeline and insulated dual-reporter system to predict and quantify strong terminators from RNA-seq datasets for metabolic engineering.","authors":"Yunhao Chen, Qiaoning He, Qiushi Ye, Jiapeng Li, Changzheng Li, Shihui Yang","doi":"10.1016/j.tibtech.2025.11.017","DOIUrl":"10.1016/j.tibtech.2025.11.017","url":null,"abstract":"The limited availability of genetic regulatory parts, especially terminators from non-model microorganisms, hampers precise gene regulation and the construction of efficient microbial cell factories. To address this problem, we developed a novel strategy for predicting and quantifying strong terminators genome-widely by integrating RNA-seq datasets with an insulated dual-reporter system. We validated 48, and 41 strong terminators in Zymomonas mobilis and Escherichia coli, respectively. Our results demonstrated notable cross-species compatibility of these terminators between the two bacteria and revealed that the length of the upstream sequence affects termination efficiency in Z. mobilis. We further applied selected strong terminators to enhance 2,3-butanediol production in Z. mobilis. This work thus establishes a pipeline for genome-wide prediction, quantitative assessment, and cross-species evaluation of bacterial terminators. It provides a valuable resource for expanding prokaryotic terminator libraries and engineering efficient microbial cell factories with refined transcriptional control.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"1452-1477"},"PeriodicalIF":14.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145896892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ engineering of synthetic yeast consortia for cross-species metabolic conversion of crude glycerol and byproducts into circular renewable bioenergy. 粗甘油及其副产物跨物种代谢转化为循环可再生生物能源的合成酵母菌群原位工程。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2026-01-09 DOI: 10.1016/j.tibtech.2025.12.002

Xiaoyu Shi, Joonhee Chang, Myeong-Eun Lee, Jun Won Oh, Dong-Hyeok Hwang, Byeong-Hyeon Cho, Sung Ok Han

{"title":"In situ engineering of synthetic yeast consortia for cross-species metabolic conversion of crude glycerol and byproducts into circular renewable bioenergy.","authors":"Xiaoyu Shi, Joonhee Chang, Myeong-Eun Lee, Jun Won Oh, Dong-Hyeok Hwang, Byeong-Hyeon Cho, Sung Ok Han","doi":"10.1016/j.tibtech.2025.12.002","DOIUrl":"10.1016/j.tibtech.2025.12.002","url":null,"abstract":"The rapid growth of biodiesel production generates large amounts of crude glycerol, a low-value byproduct with environmental and economic challenges. Here, we present an engineered yeast coculture system combining Saccharomyces cerevisiae and Yarrowia lipolytica to convert crude glycerol into isopropanol, a liquid organic hydrogen carrier. The system integrates metabolic engineering, cell surface display pairing, immobilization, and continuous cultivation in fibrous bed bioreactors. In S. cerevisiae, glycerol use was improved by transporter optimization, pathway redirection, and flux shift from ethanol to isopropanol. In Y. lipolytica, ethanol from S. cerevisiae was redirected to isopropanol by acetyl-CoA reinforcement, malonyl-CoA diversion, and NADPH availability. Optimized pairing and inoculation ratios enhanced stability and yield. The consortia achieved complete glycerol utilization and three reuse cycles over 180 h. With pure glycerol, 28.34 g/l isopropanol was produced, while crude glycerol reached 86.06% of this yield. This strategy offers a scalable, modular route to convert biodiesel byproducts into bioenergy carriers.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"1354-1377"},"PeriodicalIF":14.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards scalable anammox: mechanistic insights and emerging strategies. 向可扩展厌氧氨氧化：机制的见解和新兴战略。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2025-09-18 DOI: 10.1016/j.tibtech.2025.08.013

Yongguang Li, Yonggang Li, Bing-Jie Ni, Yue-Zhong Li, Shou-Qing Ni

引用次数: 0

Rewiring holobiont systems with synthetic biology. 用合成生物学重新布线全息生物系统。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2025-10-17 DOI: 10.1016/j.tibtech.2025.09.017

Jasdeep S Ghataora, Tom Ellis

引用次数: 0

Systematic engineering of Micromonospora echinospora cell factory for gentamicin C1a overproduction. 庆大霉素C1a过量生产小单孢子虫细胞工厂的系统工程。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2026-01-19 DOI: 10.1016/j.tibtech.2025.12.006

Feng Xu, Hao Gao, Rong Ben, Kaihao Hu, Yuan Wang, Ali Mohsin, Yuanxin Guo, Xu Li, Haifeng Hang, Ju Chu, Xiwei Tian

{"title":"Systematic engineering of Micromonospora echinospora cell factory for gentamicin C1a overproduction.","authors":"Feng Xu, Hao Gao, Rong Ben, Kaihao Hu, Yuan Wang, Ali Mohsin, Yuanxin Guo, Xu Li, Haifeng Hang, Ju Chu, Xiwei Tian","doi":"10.1016/j.tibtech.2025.12.006","DOIUrl":"10.1016/j.tibtech.2025.12.006","url":null,"abstract":"The clinical importance of gentamicin C1a as a broad-spectrum aminoglycoside antibiotic underscores the need for efficient biomanufacturing strategies. In this study, we developed a systematic engineering framework to enhance gentamicin C1a production. First, a genome-scale metabolic model (iFX1172) was reconstructed to pinpoint critical bottlenecks in both regulatory and biosynthetic pathways. Guided by model predictions and experimental validation, we identified genC, metK, and BldD as synergistic targets. Coordinated overexpression of these genes increased gentamicin C1a titers to 198.1 mg/L, representing a 34.3% improvement over the parental strain, and also enhanced the titers of other aminoglycoside antibiotics by up to 1.6-fold, demonstrating the universality of the strategy. Metabolic flux analysis and targeted metabolomics revealed that redox homeostasis and ATP availability are pivotal for biosynthesis. Finally, process optimization in a fed-batch bioreactor using a Bayesian framework, coupled with in situ resin adsorption, yielded 964.1 mg/L gentamicin C1a with a yield of 24.1 mg/g glucose and a productivity of 6.7 mg/L/h.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"1397-1424"},"PeriodicalIF":14.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146012655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Flying seed-inspired sensors for remote environmental monitoring on Earth and beyond. 以种子为灵感的飞行传感器，用于地球内外的远程环境监测。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2026-05-01 Epub Date: 2025-09-06 DOI: 10.1016/j.tibtech.2025.08.005

Sagar Arya, Lukáš Spíchal, Radek Zbořil

引用次数: 0