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Engineering Salmonella as an immune-metabolic modulator of the tumor microenvironment. 工程沙门氏菌作为肿瘤微环境的免疫代谢调节剂。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-03-11 DOI: 10.1016/j.tibtech.2026.01.010
Kontham Kulangara Varsha, Mohammed Dwidar
{"title":"Engineering Salmonella as an immune-metabolic modulator of the tumor microenvironment.","authors":"Kontham Kulangara Varsha, Mohammed Dwidar","doi":"10.1016/j.tibtech.2026.01.010","DOIUrl":"10.1016/j.tibtech.2026.01.010","url":null,"abstract":"<p><p>Engineering bacteria as cancer therapeutics is gaining mounting attention due to the remarkable success in recent preclinical studies against several solid tumor models. Among the bacteria used, Salmonella stands out due to its inherent tumor localization ability, potential to activate the immune system, and ability to cause tumor regression, while having an easily editable genetic system. Here, we gather information on the mechanisms that enable Salmonella to stimulate the immune system and exert antitumor effects. We also address the genetic manipulation approaches adopted to reduce virulence, enhance tumor colonization, and equip Salmonella with the ability to deliver immunotherapeutic payloads, along with recent advances made in tumor metabolome rewiring. Finally, we propose the challenges associated with bacteria-based cancer therapy.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2941-2956"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13151891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147445173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A catabolic powerhouse for biorefineries: characterization and engineering Erwinia spp. strain LJJL01 to produce bioproducts. 生物炼制的分解代谢发电站：Erwinia spp.菌株LJJL01的表征和工程生产生物产品。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-04-19 DOI: 10.1016/j.tibtech.2026.03.022
Lakshika Dissanayake, Bhagya S Kolitha, Sandipty Kayastha, Abigail Sparks, Ryaan Ligon, Saptarshi Ghosh, Dushmantha Madushanka, Sandhya Jayasekara, Isabelle Hou, Elwood Sheflin, Jeffrey G Linger, Scott D Hamilton-Brehm, Lahiru N Jayakody
{"title":"A catabolic powerhouse for biorefineries: characterization and engineering Erwinia spp. strain LJJL01 to produce bioproducts.","authors":"Lakshika Dissanayake, Bhagya S Kolitha, Sandipty Kayastha, Abigail Sparks, Ryaan Ligon, Saptarshi Ghosh, Dushmantha Madushanka, Sandhya Jayasekara, Isabelle Hou, Elwood Sheflin, Jeffrey G Linger, Scott D Hamilton-Brehm, Lahiru N Jayakody","doi":"10.1016/j.tibtech.2026.03.022","DOIUrl":"10.1016/j.tibtech.2026.03.022","url":null,"abstract":"<p><p>Nonmodel microbial hosts can efficiently biotransform unconventional organic feedstocks into advanced bioproducts, leveraging their superior metabolic capabilities and resilience to process-relevant physicochemical conditions. In this study, we domesticated Erwinia spp. strain. LJJL01 (Er LJJL01) as a potent microbial chassis to advance the emerging biorefinery strategy for the valorization of lignin-rich biomass and plastic, thereby enabling the circular economy. The strain exhibits remarkable chemical tolerance and can biofunnel various substrates, including sugars, acids, polyols, and aromatics, in minimal salt media to produce native fine chemicals such as acetoin, 2,3-butanediol, and lactic acid. As a proof of concept, engineering this strain with advanced genetic tools enables the production of tailored high-value chemicals, such as cis,cis-muconate from plastic-derived terephthalate and polyhydroxybutyrate from lignocellulosic hydrolysate. We established Er LJJL01 as a potent microbial chassis for the green synthesis of bioproducts from unconventional organic feedstocks.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2983-3010"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Therapeutic microneedles for myocardial repair. 用于心肌修复的治疗性微针。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-04-24 DOI: 10.1016/j.tibtech.2025.12.024
Adile Yürük, Emre Ece, Mohammad Akrami-Hasan-Kohal, Fabian Werle, Sidi A Bencherif, Fatih Inci
{"title":"Therapeutic microneedles for myocardial repair.","authors":"Adile Yürük, Emre Ece, Mohammad Akrami-Hasan-Kohal, Fabian Werle, Sidi A Bencherif, Fatih Inci","doi":"10.1016/j.tibtech.2025.12.024","DOIUrl":"10.1016/j.tibtech.2025.12.024","url":null,"abstract":"<p><p>Local myocardial delivery remains an unmet need in cardiovascular disease, as systemic therapies are hindered by cardiac motion, heterogeneous perfusion, and post-infarction extracellular matrix remodeling. Preclinical microneedle (MN) systems enable direct, localized, and sustained epicardial delivery of drugs, proteins, growth factors, and cells while minimizing systemic exposure. Recent studies have demonstrated tangible benefits: galunisertib-loaded MNs reduced fibrosis from ~14% to ~7%, and vascular endothelial growth factor (VEGF)-based MNs increased vascular density from ~0.2 to ~0.5 vessels per field. Mechanically adaptive and biodegradable MN platforms maintain stable contact with the beating heart and support programmable release profiles. This review synthesizes the current preclinical landscape of cardiac MN technologies and outlines the engineering and translational considerations that will guide their advancement toward future clinical applications.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2891-2908"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
One-pot expression of an oligoclonal nanobody-based antivenom in growth-decoupled Escherichia coli. 寡克隆纳米体抗蛇毒血清在生长解耦大肠杆菌中的单锅表达。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-04-29 DOI: 10.1016/j.tibtech.2026.02.022
Patrick Stargardt, Anne Ljungars, David Scheich, Nico Lingg, Emil S Elmose, Shirin Ahmadi, Florian Simon, Alois Jungbauer, Juergen Mairhofer, Andreas H Laustsen
{"title":"One-pot expression of an oligoclonal nanobody-based antivenom in growth-decoupled Escherichia coli.","authors":"Patrick Stargardt, Anne Ljungars, David Scheich, Nico Lingg, Emil S Elmose, Shirin Ahmadi, Florian Simon, Alois Jungbauer, Juergen Mairhofer, Andreas H Laustsen","doi":"10.1016/j.tibtech.2026.02.022","DOIUrl":"10.1016/j.tibtech.2026.02.022","url":null,"abstract":"<p><p>Oligoclonal V<sub>H</sub>H mixtures targeting distinct epitopes offer therapeutic benefits in mitigating complex diseases, including cancer, infectious diseases, and snakebite envenoming. However, current production strategies rely on separate expression and purification of individual V<sub>H</sub>Hs, followed by downstream mixing, resulting in high costs. We present a cocultivation approach for producing an experimental recombinant antivenom using growth-decoupled Escherichia coli strains. Six different cell lines were generated by transforming the same host with different V<sub>H</sub>H genes. The cell lines displayed high-yield extracellular secretion in microtiter plate and 1-l bioreactor cultivations, and cocultivation at defined inoculation ratios yielded oligoclonal V<sub>H</sub>H mixtures approaching target compositions. This strategy enables one-pot manufacturing with minimal downstream processing and predictable composition. A techno-economic assessment based on obtained titers and two-step purification indicates reduced per-treatment costs compared with Chinese Hamster Ovary cells produced IgG-based antivenoms. Our findings offer an alternative to conventional oligoclonal V<sub>H</sub>H production for multivalent therapeutics requiring broad neutralization, including recombinant antivenoms.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"3126-3141"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Revolutionizing vision using corneal 3D printing. 使用角膜3D打印革新视力。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-03-18 DOI: 10.1016/j.tibtech.2026.01.019
Bowen Zhang, F A Dain Md Opo, Daniel Nahra, James Chodosh, Miguel Gonzalez-Andrades, Mohammad Mirazul Islam, Hirak K Patra
{"title":"Revolutionizing vision using corneal 3D printing.","authors":"Bowen Zhang, F A Dain Md Opo, Daniel Nahra, James Chodosh, Miguel Gonzalez-Andrades, Mohammad Mirazul Islam, Hirak K Patra","doi":"10.1016/j.tibtech.2026.01.019","DOIUrl":"10.1016/j.tibtech.2026.01.019","url":null,"abstract":"<p><p>Corneal diseases remain a leading cause of blindness, and corneal transplantation is the primary treatment for severe cases. However, the persistent shortage of donor corneas significantly limits this approach. Artificial corneas with engineered biomaterials designed to mimic the structure and function of native corneal tissue offer a promising alternative, and significant progress has been made, including FDA approval and clinical trials. Among emerging technologies, 3D printing enables personalized customization of implants tailored to patient needs, anatomy, and pathology. This method also enables precise control over the biomaterial composition and integration of living cells within the implant, enhancing post-transplantation regeneration. In this review, we examine the advancements in 3D-printed, proregenerative artificial corneas, focusing on innovative biofabrication and the challenges that remain in ensuring implant quality.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2849-2869"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13570457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147487226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human brain organoids and stakeholders' attitudes: evidence, gaps, and governance. 人脑类器官和利益相关者的态度：证据、差距和治理。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-03-25 DOI: 10.1016/j.tibtech.2026.01.001
Kiichi Inarimori, Masanori Kataoka, Koji Ota, Brian D Earp, Julian Savulescu, Tsutomu Sawai
{"title":"Human brain organoids and stakeholders' attitudes: evidence, gaps, and governance.","authors":"Kiichi Inarimori, Masanori Kataoka, Koji Ota, Brian D Earp, Julian Savulescu, Tsutomu Sawai","doi":"10.1016/j.tibtech.2026.01.001","DOIUrl":"10.1016/j.tibtech.2026.01.001","url":null,"abstract":"<p><p>We review 13 empirical studies examining attitudes toward human brain organoid (HBO) research. Stakeholders tend to emphasize practical concerns-worries about commercialization, reproductive cloning, informed consent, and uncertainty about consequences-rather than issues related to consciousness. Based on these findings, we identify three priority areas for future ethical and policy discussions.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2817-2820"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147520865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Volumetric 3D printing of microalgae-based engineered living materials. 基于微藻的工程生物材料的体积3D打印。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-09-08 DOI: 10.1016/j.tibtech.2026.08.016
Christoph A Spiegel, Eva Blasco
{"title":"Volumetric 3D printing of microalgae-based engineered living materials.","authors":"Christoph A Spiegel, Eva Blasco","doi":"10.1016/j.tibtech.2026.08.016","DOIUrl":"10.1016/j.tibtech.2026.08.016","url":null,"abstract":"<p><p>In their work, Windisch et al. demonstrate the successful adaptation of volumetric 3D printing to photosynthetically active green microalgae-based engineered living materials (ELMs), addressing key fabrication challenges associated with light scattering and absorption in cell-laden materials. Their work expands the fabrication toolbox for geometrically complex ELMs and highlights volumetric printing as a promising approach for developing functional photosynthetic living materials.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2824-2826"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148907273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toward adaptive cyborg tissues. 向适应性半机械人组织发展。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 DOI: 10.1016/j.tibtech.2026.09.009
Qiang Li, Jeantine Lunshof, Jia Liu
{"title":"Toward adaptive cyborg tissues.","authors":"Qiang Li, Jeantine Lunshof, Jia Liu","doi":"10.1016/j.tibtech.2026.09.009","DOIUrl":"https://doi.org/10.1016/j.tibtech.2026.09.009","url":null,"abstract":"<p><p>Organoids and engineered tissues have emerged as powerful models of human biology, yet their development and function are often characterized through endpoint measurements that provide only static snapshots of dynamic biological processes. Recent advances in stretchable mesh electronics, developmental biointegration, and organoid engineering have enabled the creation of cyborg tissues with embedded sensing, stimulation, and computational capabilities. This review highlights recent progress in developmental bioelectronic integration, multifunctional tissue mapping, biological state reconstruction, and artificial intelligence (AI)-enabled cyborg systems. We discuss how embedded bioelectronics can be combined with molecular and spatial profiling technologies to characterize tissue function, developmental trajectories, and biological states. These advances establish cyborg tissues as biologically integrated platforms for regenerative medicine, disease modeling, drug discovery, and adaptive biotechnology.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of titer, rate, yield, and scale on the cost and life-cycle emissions of biomanufacturing. 滴度、速率、产量和规模对生物制造成本和生命周期排放的影响。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-04-17 DOI: 10.1016/j.tibtech.2026.03.023
Nawa Raj Baral, Minliang Yang, Deepanwita Banerjee, Aindrila Mukhopadhyay, Blake A Simmons, Steven W Singer, Jay D Keasling, Corinne D Scown
{"title":"Influence of titer, rate, yield, and scale on the cost and life-cycle emissions of biomanufacturing.","authors":"Nawa Raj Baral, Minliang Yang, Deepanwita Banerjee, Aindrila Mukhopadhyay, Blake A Simmons, Steven W Singer, Jay D Keasling, Corinne D Scown","doi":"10.1016/j.tibtech.2026.03.023","DOIUrl":"10.1016/j.tibtech.2026.03.023","url":null,"abstract":"<p><p>Building a thriving bioeconomy requires microbial hosts that efficiently convert diverse carbon sources at high titer, rate, and yield (TRY). Yet, the impacts of TRY on biofuel production cost and carbon footprint across different stages of strain optimization remain unclear. This study presents a systematic analysis of the individual and combined impacts of TRY and scale for the production of multiple bio-based products, including medium-chain (C13-C15) methyl ketones, bisabolene, and ethanol, from lignocellulosic biomass hydrolysates. The results show nonlinear behavior in both cost and CO<sub>2</sub>-equivalent footprints as a function of TRY, revealing thresholds and inflection points that can inform research and set better goals for commercialization. Ultimately, selecting process-based biorefinery size, including the production cost and carbon mitigation credits, and achieving near-theoretical yield with a titer and rate of >130 g/l and >2 g/l/h, respectively, are essential to produce economically viable biofuels with low life-cycle emissions.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"3011-3033"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147718387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant-based models of neurodegenerative CAG repeat expansion diseases. 神经退行性CAG重复扩张病的植物模型。
IF 16.6 1区 工程技术
Trends in biotechnology Pub Date : 2026-10-01 Epub Date: 2026-03-14 DOI: 10.1016/j.tibtech.2026.01.003
Mythili Saravanan, Farooqahmed S Kittur, Jiahua Xie
{"title":"Plant-based models of neurodegenerative CAG repeat expansion diseases.","authors":"Mythili Saravanan, Farooqahmed S Kittur, Jiahua Xie","doi":"10.1016/j.tibtech.2026.01.003","DOIUrl":"10.1016/j.tibtech.2026.01.003","url":null,"abstract":"<p><p>Expansion of cytosine-adenine-guanine (CAG) trinucleotide repeats causes Huntington's disease and other neurodegenerative disorders, yet no disease-modifying treatments exist. Plant-based CAG models expressing mutant huntingtin protein provide novel insights into expanded CAG-induced toxicity, complementing traditional systems for studying neurodegeneration and therapeutic discovery, and may offer unique opportunities for advancing plant biology research.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2831-2834"},"PeriodicalIF":16.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12991418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147460410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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