Trends in biotechnology最新文献_第2页

CRISPR genome editing in plants without tissue culture. 无组织培养植物的CRISPR基因组编辑。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-02-16 DOI: 10.1016/j.tibtech.2025.12.017

Chao Li, Desheng Mei, Hongtao Cheng, Xiaoping Pan, Baohong Zhang

引用次数: 0

Engineering Bacillus subtilis as a sustainable platform for the production of functional bile acids. 工程枯草芽孢杆菌作为生产功能性胆汁酸的可持续平台。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-04-15 DOI: 10.1016/j.tibtech.2026.03.021

Yingchao Zang, Zhongpeng Guo, Rodrigo Ledesma-Amaro, Yu Xin, Yaokang Wu, Zhenghua Gu, Liang Zhang

{"title":"Engineering Bacillus subtilis as a sustainable platform for the production of functional bile acids.","authors":"Yingchao Zang, Zhongpeng Guo, Rodrigo Ledesma-Amaro, Yu Xin, Yaokang Wu, Zhenghua Gu, Liang Zhang","doi":"10.1016/j.tibtech.2026.03.021","DOIUrl":"10.1016/j.tibtech.2026.03.021","url":null,"abstract":"Bile acids and their derivatives are key regulators of host metabolism, immunity, and microbiome interactions, with growing therapeutic potential. Conventional production methods of bile acids relying on animal extraction or chemical synthesis are inefficient, costly, and environmentally unfriendly. Microbial synthesis offers a sustainable alternative but is limited by the absence of genetic tools for native anaerobic producers. Here, we reconstructed the bile acid 7α-dehydroxylation pathway in Bacillus subtilis, a safe and tractable host. We characterized the key Bai enzymes and performed comprehensive analyses of the bile acids generated through in vitro assays and whole-cell catalysis. Multiple intermediates and end products, including cholyl-CoA, 3-oxo-cholic acid, 3-oxo-4,5-6,7-didehydro-deoxycholic acid, 3-oxo-4,5-dehydro-deoxycholic acid, 3-oxo-deoxycholic acid, and deoxycholic acid, were identified. This work establishes a functional heterologous platform for bile acid biosynthesis, enabling sustainable production and future applications in therapeutics and microbiome engineering.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2679-2698"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadherin-engineered microspheres enable scalable biofabrication of multilineage liver organoids. 钙粘蛋白工程微球使多谱系类肝器官的可扩展生物制造成为可能。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-04-15 DOI: 10.1016/j.tibtech.2026.03.014

Zheng Qin, Lei Cao, Chang Liu, Yong An, Fangshuo Li, Xipeng Yang, Guoqiang Chen, Mengyuan Qian, Yan Zhang, Jun Yang

{"title":"Cadherin-engineered microspheres enable scalable biofabrication of multilineage liver organoids.","authors":"Zheng Qin, Lei Cao, Chang Liu, Yong An, Fangshuo Li, Xipeng Yang, Guoqiang Chen, Mengyuan Qian, Yan Zhang, Jun Yang","doi":"10.1016/j.tibtech.2026.03.014","DOIUrl":"10.1016/j.tibtech.2026.03.014","url":null,"abstract":"Reproducing the spatiotemporally coordinated emergence of vascular, biliary, and parenchymal compartments of the human liver remains a persistent bottleneck in organoid engineering. We introduce biodegradable poly(lactic-co-glycolic acid) microspheres that display epithelial (E) cadherin and vascular endothelial (VE) cadherin fusion proteins and deliver morphogens, mimicking key developmental signals that guide liver organogenesis. When combined with mesenchymal stem cells (MSCs), the microspheres supply adhesion cues that drive aggregate compaction. E-cadherin engagement induces Yes-associated protein activation, priming MSCs for lineage induction. Complementarily, VE-cadherin and vascular endothelial growth factor jointly establish an endothelial niche that delivers inductive cues for hepatic lineage divergence. The resulting organoids display compartmentalized architecture comprising hepatocytes, cholangiocytes, mesenchymal cells, and endothelial networks and respond to hepatotoxic compounds with clinically relevant phenotypes. Unlike induced pluripotent stem cell-based or multiple cell cocultures, this single-source MSC system achieves self-organization through cadherin-defined adhesion and timed morphogen delivery. These elements define a scalable, cadherin-defined biomaterial platform for liver organoid engineering, enabling translational use in drug evaluation and regenerative medicine.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2744-2770"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trehalose 6-phosphate as master switch for optimizing crop yield and resilience. 海藻糖6-磷酸作为优化作物产量和抗逆性的总开关。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-02-19 DOI: 10.1016/j.tibtech.2025.12.029

Muhammad Waqas, Chen Yaning, Yong-Ling Ruan, Zi-Ming Ren, Mark Stitt, Christoph-Martin Geilfus

引用次数: 0

Machine learning for designing low-risk microbial consortia pesticides. 设计低风险微生物群落杀虫剂的机器学习。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-03-17 DOI: 10.1016/j.tibtech.2025.12.027

Mónica Garcés-Ruiz, Belén Guijarro Díaz-Otero, Livio Antonielli, João Pedro Saraiva, Dimitrios Karpouzas, Stéphane Declerck

引用次数: 0

A universal light-controlled highly sensitive one-pot CRISPR/Cas12a diagnostic based on structure-engineered crRNA. 基于结构工程crRNA的通用光控高灵敏度单锅CRISPR/Cas12a诊断。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-04-11 DOI: 10.1016/j.tibtech.2026.03.018

Jieyu Cui, Lulu Zhang, Jing Zhou, Ting Shi, Shangyi Wu, Tenghui Dai, Lijun Hao, Junjie Pan, Xiaodan Lai, Wentao Lu, Xingxu Huang, Zhanjun Li, Liangxue Lai, Xinjie Wang

{"title":"A universal light-controlled highly sensitive one-pot CRISPR/Cas12a diagnostic based on structure-engineered crRNA.","authors":"Jieyu Cui, Lulu Zhang, Jing Zhou, Ting Shi, Shangyi Wu, Tenghui Dai, Lijun Hao, Junjie Pan, Xiaodan Lai, Wentao Lu, Xingxu Huang, Zhanjun Li, Liangxue Lai, Xinjie Wang","doi":"10.1016/j.tibtech.2026.03.018","DOIUrl":"10.1016/j.tibtech.2026.03.018","url":null,"abstract":"Clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection has transformed molecular diagnostics through its speed and accuracy; however, one-pot formats are often limited by sensitivity and field suitability. Herein, we developed a universal light-controlled high-sensitivity one-pot CRISPR/Cas12a testing (ULTRAt) platform based on structure-engineered CRISPR RNA (crRNA) scaffolds. By incorporating photocaged 6-nitropiperonyloxymethyl groups into the crRNA stem-loop, Cas12a activity is transiently suppressed during isothermal amplification via structural modulation, enabling efficient target enrichment. Subsequent UV irradiation removes the protecting groups, restoring the native conformation and activating robust trans-cleavage. ULTRAt achieves a limit of detection of two copies of monkeypox virus per reaction with a 15-min time-to-result, representing a 100-fold sensitivity improvement over conventional assays. The platform further supports single-nucleotide polymorphism discrimination and human papillomavirus 16/18 genotyping. Analysis of 91 clinical samples demonstrates strong concordance between ULTRAt and reference qPCR and sequencing assays. Collectively, ULTRAt enables rapid, ultra-sensitive, and versatile one-pot detection, supporting near-patient diagnostics and genotyping.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2699-2721"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paenibacillus polymyxa cell factories for production of polymyxins. 多粘菌芽孢杆菌生产多粘菌素的细胞工厂。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-03-23 DOI: 10.1016/j.tibtech.2025.12.021

Cai-Yun Wu, Man-Man Xu, Jie Li, Tian-Qiong Shi

引用次数: 0

Advancements and challenges in CAR-NK cell therapy for cancer treatment. CAR-NK细胞治疗癌症的进展和挑战。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-02-16 DOI: 10.1016/j.tibtech.2025.12.020

Zhengshui Xu, Yu Li, Yundong Zhou, Changchun Ye, Junjie Kuang, Chengliang Yin, Danwen Zhao, Hui Guo, Narmatha Chellamani, Manimurugan Shanmuganathan, João Conde, Shiyuan Liu

引用次数: 0

Dynamic control of integrin signaling for cell manipulation via molecularly imprinted decoy receptor. 分子印迹诱饵受体对细胞操纵整合素信号的动态控制。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-04-10 DOI: 10.1016/j.tibtech.2026.03.015

Fan Ding, Jingjing Xu, Yue Ma, Zhaoyang Yao, Jia Gao, Kangni Shang, Karsten Haupt, Guoqing Pan

{"title":"Dynamic control of integrin signaling for cell manipulation via molecularly imprinted decoy receptor.","authors":"Fan Ding, Jingjing Xu, Yue Ma, Zhaoyang Yao, Jia Gao, Kangni Shang, Karsten Haupt, Guoqing Pan","doi":"10.1016/j.tibtech.2026.03.015","DOIUrl":"10.1016/j.tibtech.2026.03.015","url":null,"abstract":"Integrin plays a crucial role in human immune system activation and stem cell differentiation through interactions with the extracellular matrix (ECM). Reversible modulation of integrin signaling has illuminated the road toward broad-spectrum disease treatment and tissue regeneration. In this regard, an integrin-like decoy receptor, termed molecularly imprinted polymer (MIP), was synthesized via solid-phase imprinting to target the Arg-Gly-Asp (RGD) sequence, an integrin-binding motif abundantly present in the ECM. The MIP, with temperature-dependent RGD affinity, enabled dynamic intervention in integrin signaling, thereby modulating integrin-RGD-mediated immune response and cell differentiation. The MIP could, on demand, orchestrate macrophage phenotypes to display proinflammatory or anti-inflammatory traits, potentially beneficial at the early stage of tissue injury. Furthermore, stem cell fates could be controlled by MIP, showing potential to prevent hyperplasia caused by excessive differentiation. The proof-of-concept studies demonstrate the feasibility of MIP-based decoy receptors in the dynamic control of integrin signals and cell evolution, showing promise in tissue repair and related diseases.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2796-2811"},"PeriodicalIF":16.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic and enzyme engineering for steroid hormone biosynthesis. 类固醇激素生物合成的代谢与酶工程。

IF 16.6 1区工程技术

Trends in biotechnology Pub Date : 2026-09-01 Epub Date: 2026-01-23 DOI: 10.1016/j.tibtech.2025.12.012

Qihang Chen, Changtai Zhang, Song Liu, Weizhou Zeng, Jingwen Zhou

引用次数: 0