基于蛋白质的分子印迹:明胶纳米陷阱用于炎症细胞模型中白细胞介素-6的隔离。

IF 14.3 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Trends in biotechnology Pub Date : 2025-05-01 Epub Date: 2025-03-06 DOI:10.1016/j.tibtech.2025.02.002
Alessandra Maria Bossi, Sofia Casella, Chiara Stranieri, Alice Marinangeli, Alessio Bucciarelli, Anna Maria Fratta Pasini, Devid Maniglio
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引用次数: 0

摘要

尽管蛋白质衍生的生物材料具有生物相容性和安全的生物可降解性等诸多优点,但它们在分子印迹技术中的作用目前被低估了。明胶是一种生物聚合物,它可以很容易地用悬垂双键进行修饰,从而进行聚合,使其适合于组织工程和生物制造。在这项研究中,我们使用明胶甲基丙烯酰(GelMA)作为构建块,结合分子印迹技术,创建了一类原始的生物启发纳米陷阱,专门能够隔离促炎细胞因子白细胞介素-6 (IL-6)。研究了纳米捕集器在溶液中的稳定性、生物相容性和生物降解性。纳米陷阱对IL-6具有选择性和特异性,显示出纳米摩尔亲和力,并且在体外炎症细胞模型上测试时,具有剂量-反应关系的隔离IL-6。总之,我们的研究表明,蛋白质化学驱动的分子印迹可以更广泛地用于设计生物相容性的功能纳米材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protein-based molecular imprinting: gelatin nanotraps for interleukin-6 sequestration in inflammation cell models.

Protein-derived biomaterials are currently underrated as building blocks in molecular imprinting, even though they offer several benefits, such as biocompatibility and safe biodegradability. Gelatin is a biopolymer that can be easily modified with pendant double bonds for polymerization, making it suitable for tissue engineering and biofabrication. In this study, we used gelatin methacryloyl (GelMA) as a building block combined with molecular imprinting technology to create an original class of bioinspired nanotraps specifically capable of sequestering the proinflammatory cytokine interleukin-6 (IL-6). The stability in solution, biocompatibility, and biodegradability of the nanotraps were assessed. The nanotraps were selective and specific for IL-6, showing nanomolar affinity and, when tested in vitro on an inflammation cell model, sequestered IL-6 with a dose-response relationship. Overall, our study shows that protein chemistry-driven molecular imprinting could become more widely used to devise biocompatible functional nanomaterials.

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来源期刊
Trends in biotechnology
Trends in biotechnology 工程技术-生物工程与应用微生物
CiteScore
28.60
自引率
1.20%
发文量
198
审稿时长
1 months
期刊介绍: Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems. The major themes that TIBTECH is interested in include: Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering) Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology) Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics) Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery) Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).
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