Bottom-up development of lipid-based synthetic cells for practical applications.

Synthetic cells (SCs) can be engineered from the bottom up to recapitulate the functional properties of natural cells while performing specialized tasks such as drug delivery, biosensors, bioproduction, vaccine development, and even environmental remediation. Recent advances in synthetic biology, biomaterials, and microfluidics have enabled the development of increasingly sophisticated SCs. Transitioning from proof-of-concept demonstrations to practical applications requires a deep understanding of the design principles, materials, and fabrication techniques involved. This review provides a comprehensive overview of the current state of bottom-up SC technology and highlights the most promising approaches and applications. Challenges in the implementation of SCs and their prospects for future applications are also discussed.