Trends in biotechnology最新文献_第7页

Biomimetic chemical microhabitats enhance coral settlement. 仿生化学微生境促进珊瑚沉降。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-05-14 DOI: 10.1016/j.tibtech.2025.03.019

Samapti Kundu, Simone Potenti, Zachary A Quinlan, Helena Willard, Justin Chen, Timothy Noritake, Natalie Levy, Zahra Karimi, Hendrikje Jorissen, Joshua R Hancock, Crawford Drury, Linda Wegley Kelly, Luisa De Cola, Shaochen Chen, Daniel Wangpraseurt

{"title":"Biomimetic chemical microhabitats enhance coral settlement.","authors":"Samapti Kundu, Simone Potenti, Zachary A Quinlan, Helena Willard, Justin Chen, Timothy Noritake, Natalie Levy, Zahra Karimi, Hendrikje Jorissen, Joshua R Hancock, Crawford Drury, Linda Wegley Kelly, Luisa De Cola, Shaochen Chen, Daniel Wangpraseurt","doi":"10.1016/j.tibtech.2025.03.019","DOIUrl":"10.1016/j.tibtech.2025.03.019","url":null,"abstract":"Anthropogenic stressors pose substantial threats to the existence of coral reefs. Achieving successful coral recruitment stands as a bottleneck in reef restoration and hybrid reef engineering efforts. Here, we enhance coral settlement through the development of biomimetic microhabitats that replicate the chemical landscape of healthy reefs. We engineered a soft biomaterial, SNAP-X, comprising silica nanoparticles (NPs), biopolymers, and algal exometabolites, to enrich reef microhabitats with bioactive molecules from crustose coralline algae (CCA). Coral settlement was enhanced over 20-fold using SNAP-X-coated substrates compared with uncoated controls. SNAP-X is designed to release chemical signals slowly (>1 month) under natural seawater conditions, and can be rapidly applied to natural reef substrates via photopolymerization, facilitating the light-assisted 3D printing of microengineered habitats. We anticipate that these biomimetic chemical microhabitats will be widely used to augment coral settlement on degraded reefs and to support ecosystem processes on hybrid reefs.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2232-2250"},"PeriodicalIF":14.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing wheat breeding using rye: a key contribution to wheat breeding history. 利用黑麦推进小麦育种：对小麦育种史的重大贡献。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-04-07 DOI: 10.1016/j.tibtech.2025.03.008

Guohao Han, Hanwen Yan, Lihui Li, Diaoguo An

引用次数: 0

Non-viable embryos created with synthetic DNA. 用合成DNA制造的不能存活的胚胎。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-03-24 DOI: 10.1016/j.tibtech.2025.02.021

Adrian Villalba, Anna Smajdor, Iain Brassington, Daniela Cutas

引用次数: 0

Robotic micromotors transforming oral drug administration. 机器人微型马达改变口服药物管理。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-04-12 DOI: 10.1016/j.tibtech.2025.03.011

Amal Abbas, Rodolfo Mundaca-Uribe, Liangfang Zhang, Joseph Wang

引用次数: 0

Splice, exchange, extend: precision editing of native proteins in live cells. 剪接、交换、扩展：活细胞中天然蛋白的精确编辑。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-08-02 DOI: 10.1016/j.tibtech.2025.07.021

Kevin Schiefelbein, Yael David

引用次数: 0

Production of phosphorylated and functional α_s1-casein in Escherichia coli. 在大肠杆菌中产生磷酸化和功能性αs1酪蛋白。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-07-02 DOI: 10.1016/j.tibtech.2025.05.015

Suvasini Balasubramanian, Golnaz Mobasseri, Lei Shi, Carsten Jers, Julie Bonne Køhler, Adeline Boire, Claire Berton-Carabin, Ivan Mijakovic, Peter Ruhdal Jensen

{"title":"Production of phosphorylated and functional αs1-casein in Escherichia coli.","authors":"Suvasini Balasubramanian, Golnaz Mobasseri, Lei Shi, Carsten Jers, Julie Bonne Køhler, Adeline Boire, Claire Berton-Carabin, Ivan Mijakovic, Peter Ruhdal Jensen","doi":"10.1016/j.tibtech.2025.05.015","DOIUrl":"10.1016/j.tibtech.2025.05.015","url":null,"abstract":"While the demand for milk proteins is on the rise, sustainability concerns dictate a reduction in animal-based agriculture. Microbial cell factories can be a viable alternative, but their inability to phosphorylate recombinant caseins is a major bottleneck, since phosphorylation is needed for calcium binding and functionality. We propose a solution that involves engineering bacteria to co-express bacterial protein kinases, resulting in recombinant caseins with the native phosphorylation pattern and functional properties. We successfully phosphorylated αs1-casein in Escherichia coli using bacterial kinases, achieving phosphorylation at all native sites. To complement this approach, we developed an alternative phosphomimetic strategy by substituting serine residues with aspartate to mimic phosphorylation. Structural and functional characterization of all the phosphorylated/phosphomimetic recombinant αs1-caseins demonstrated properties comparable with those of bovine α-casein, including calcium-binding affinity, digestibility, and structural integrity. Notably, phosphomimetic αs1-casein may offer a simpler system, while phosphorylated casein more closely resembles bovine casein. This approach has significant implications for the development of alternative protein sources, addressing both sustainability and functional demands in the food industry.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2304-2322"},"PeriodicalIF":14.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vector-free intra-airway in vivo epigenetic editing. 无载体气道内体内表观遗传编辑。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-07-02 DOI: 10.1016/j.tibtech.2025.05.007

Naohiro Yano, Mohankumar Ramar, David J Gregory, Alexey V Fedulov

{"title":"Vector-free intra-airway in vivo epigenetic editing.","authors":"Naohiro Yano, Mohankumar Ramar, David J Gregory, Alexey V Fedulov","doi":"10.1016/j.tibtech.2025.05.007","DOIUrl":"10.1016/j.tibtech.2025.05.007","url":null,"abstract":"Targeted, promoter-specific removal of DNA methylation marks is emerging as a promising strategy for experimental and therapeutic regulation of gene expression. Research to date has relied largely on the expression of transgene-encoded epigenetic editor constructs in target cells. While effective for in vitro demonstrations, alternative approaches are needed for greater translatability to humans. Here, we describe the design of recombinant, gene-targeted epigenetic editor proteins that are directly taken up by mouse lung cells following administration in vivo without transgenesis, vectors, or packaging tools. Proteins are targeted to their intended promoter using either dCas9 or artificial zinc finger domains, and thymine-DNA-glycosylase (TDG) and ten-eleven translocation proteins (Tet) catalytic domains mediate specific demethylation. Results demonstrate intranuclear arrival of epigenetic editors in vitro and in mice, local DNA demethylation, and resulting highly gene-specific derepression of the transcriptional response, which confers sensitivity to interferon (IFN) stimulation. Therefore, this study provides proof of principle for vector-free, targeted promoter demethylation in vivo.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2251-2275"},"PeriodicalIF":14.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12233164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HHV-6 and HHV-7 reactivation in allogeneic CAR-T cell therapy. HHV-6和HHV-7在异基因CAR-T细胞治疗中的再激活。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-04-22 DOI: 10.1016/j.tibtech.2025.03.017

Hiu Tung Chow, Wenjing Li, Bin Yang, Friedrich von Wintzingerode, Qi Chen

引用次数: 0

Inflammation-targeting and self-limited neutrophilic membrane-encapsulated teicoplanin for the treatment of infectious pneumonia. 炎症靶向和自限性中性粒细胞膜包膜替柯普兰治疗感染性肺炎。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-06-14 DOI: 10.1016/j.tibtech.2025.05.009

Huidan Lu, Kaixin Zhang, Wanying Zhang, Wenting Zhang, Jicheng Wu, Xinliang Ming, Yuqiao Huang, Feng Xu, Ben Wang

{"title":"Inflammation-targeting and self-limited neutrophilic membrane-encapsulated teicoplanin for the treatment of infectious pneumonia.","authors":"Huidan Lu, Kaixin Zhang, Wanying Zhang, Wenting Zhang, Jicheng Wu, Xinliang Ming, Yuqiao Huang, Feng Xu, Ben Wang","doi":"10.1016/j.tibtech.2025.05.009","DOIUrl":"10.1016/j.tibtech.2025.05.009","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) pneumonia has a high clinical incidence and is associated with a significant mortality risk. The existence of intracellular pathogens and the infection-induced swarming of neutrophils exacerbate the challenges in treating pneumonia. Here, we addressed these issues by developing a platform based on neutrophilic membrane-camouflaged teicoplanin (Teic@NEV) to kill bacteria in tissue and prevent inflammatory lung injury associated with MRSA pneumonia. Teic@NEV improved the efficiency of drug entry into cells, meaningfully increasing the intracellular drug concentration in infected cells and eliminating intracellular MRSA. Moreover, Teic@NEV enhanced the penetration of teicoplanin into the biofilm and improved antimicrobial and antibiofilm activities in vitro. Surprisingly, Teic@NEV delivered teicoplanin specifically to sites of inflammation and reduced lung injury by hindering neutrophil swarming in vivo. Thus, this platform represents an effective strategy to limit neutrophil swarming and kill intracellular pathogens in patients with MRSA pneumonia, demonstrating its significant potential for use in clinical practice.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":"2276-2303"},"PeriodicalIF":14.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges of alignment in synthetic cell research. 合成细胞研究中对齐的挑战。

IF 14.9 1区工程技术

Trends in biotechnology Pub Date : 2025-09-01 Epub Date: 2025-06-20 DOI: 10.1016/j.tibtech.2025.05.024

M G J L Habets, H A E Zwart, M H Smolka

引用次数: 0