Trends in biotechnology最新文献_第7页

Power-to-vitamins: producing folate (vitamin B₉) from renewable electric power and CO₂ with a microbial protein system. 从电力到维生素：利用微生物蛋白质系统从可再生电力和二氧化碳中生产叶酸（维生素 B9）。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-22 DOI: 10.1016/j.tibtech.2024.06.014

Lisa Marie Schmitz, Nicolai Kreitli, Lisa Obermaier, Nadine Weber, Michael Rychlik, Largus T Angenent

{"title":"Power-to-vitamins: producing folate (vitamin B9) from renewable electric power and CO2 with a microbial protein system.","authors":"Lisa Marie Schmitz, Nicolai Kreitli, Lisa Obermaier, Nadine Weber, Michael Rychlik, Largus T Angenent","doi":"10.1016/j.tibtech.2024.06.014","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.06.014","url":null,"abstract":"We recently proposed a two-stage Power-to-Protein technology to produce microbial protein from renewable electric power and CO2. Two stages were operated in series: Clostridium ljungdahlii in Stage A to reduce CO2 with H2 into acetate, and Saccharomyces cerevisiae in Stage B to utilize O2 and produce microbial protein from acetate. Renewable energy can be used to power water electrolysis to produce H2 and O2. A drawback of Stage A was the need for continuous vitamin supplementation. In this study, by using the more robust thermophilic acetogen Thermoanaerobacter kivui instead of C. ljungdahlii, vitamin supplementation was no longer needed. Additionally, S. cerevisiae produced folate when grown with acetate as a sole carbon source, achieving a total folate concentration of 6.7 mg per 100 g biomass with an average biomass concentration of 3 g l-1. The developed Power-to-Vitamin system enables folate production from renewable power and CO2 with zero or negative net-carbon emissions.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Requirements to bring a medical device to market. 将医疗设备推向市场的要求。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-22 DOI: 10.1016/j.tibtech.2024.07.016

Eric J Buenz, Victoria M Wallace, Suzanne Levy Friedman

引用次数: 0

Synthetic biology approaches and bioseparations in syngas fermentation. 合成气发酵中的合成生物学方法和生物分离。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-20 DOI: 10.1016/j.tibtech.2024.07.008

Naorem Bela Devi, Gopal Pugazhenthi, Kannan Pakshirajan

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Manipulating immune activity of macrophages: a materials and mechanics perspective. 操纵巨噬细胞的免疫活性：材料与力学视角。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-17 DOI: 10.1016/j.tibtech.2024.07.009

Abigail J Clevenger, Aakanksha Jha, Erika Moore, Shreya A Raghavan

引用次数: 0

Therapeutic applications of cell engineering using mRNA technology. 利用 mRNA 技术的细胞工程治疗应用。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-16 DOI: 10.1016/j.tibtech.2024.07.012

Yujia He, Angus P R Johnston, Colin W Pouton

引用次数: 0

Valorization of lignin from aqueous-based lignocellulosic biorefineries. 水基木质纤维素生物炼制过程中木质素的增值。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-10 DOI: 10.1016/j.tibtech.2024.07.004

Xueli Chen, Nathan Mosier, Michael Ladisch

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Microbial electrosynthesis from CO₂ reaches productivity of syngas and chain elongation fermentations. 二氧化碳的微生物电合成达到合成气和链延伸发酵的生产率。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-08 DOI: 10.1016/j.tibtech.2024.06.005

Oriol Cabau-Peinado, Marijn Winkelhorst, Rozanne Stroek, Roderick de Kat Angelino, Adrie J J Straathof, Kunal Masania, Jean Marc Daran, Ludovic Jourdin

{"title":"Microbial electrosynthesis from CO2 reaches productivity of syngas and chain elongation fermentations.","authors":"Oriol Cabau-Peinado, Marijn Winkelhorst, Rozanne Stroek, Roderick de Kat Angelino, Adrie J J Straathof, Kunal Masania, Jean Marc Daran, Ludovic Jourdin","doi":"10.1016/j.tibtech.2024.06.005","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.06.005","url":null,"abstract":"Carbon-based products are essential to society, yet producing them from fossil fuels is unsustainable. Microorganisms have the ability to take up electrons from solid electrodes and convert carbon dioxide (CO2) to valuable carbon-based chemicals. However, higher productivities and energy efficiencies are needed to reach a viability that can make the technology transformative. Here, we show how a biofilm-based microbial porous cathode in a directed flow-through electrochemical system can continuously reduce CO2 to even-chain C2-C6 carboxylic acids over 248 days. We demonstrate a threefold higher biofilm concentration, volumetric current density, and productivity compared with the state of the art. Most notably, the volumetric productivity (VP) resembles those achieved in laboratory-scale and industrial syngas (CO-H2-CO2) fermentation and chain elongation fermentation. This work highlights key design parameters for efficient electricity-driven microbial CO2 reduction. There is need and room to improve the rates of electrode colonization and microbe-specific kinetics to scale up the technology.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial messengers: nucleic acid delivery by bacteria. 微生物信使：细菌传递核酸。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-07 DOI: 10.1016/j.tibtech.2024.07.010

Alison Heggie, Teresa L M Thurston, Tom Ellis

引用次数: 0

Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis. 利用高效的基因组编辑工具改造顽劣的非模式工业微生物 Zymomonas mobilis。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-06 DOI: 10.1016/j.tibtech.2024.05.005

Geng Binan, Wu Yalun, Wu Xinyan, Yang Yongfu, Zhou Peng, Chen Yunhaon, Zhou Xuan, Liu Chenguang, Bai Fengwu, Xu Ping, He Qiaoning, Yang Shihui

{"title":"Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis.","authors":"Geng Binan, Wu Yalun, Wu Xinyan, Yang Yongfu, Zhou Peng, Chen Yunhaon, Zhou Xuan, Liu Chenguang, Bai Fengwu, Xu Ping, He Qiaoning, Yang Shihui","doi":"10.1016/j.tibtech.2024.05.005","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.05.005","url":null,"abstract":"Current biotechnology relies on a few well-studied model organisms, such as Escherichia coli and Saccharomyces cerevisiae, for which abundant information and efficient toolkits are available for genetic manipulation, but which lack industrially favorable characteristics. Non-model industrial microorganisms usually do not have effective and/or efficient genome-engineering toolkits, which hampers the development of microbial cell factories to meet the fast-growing bioeconomy. In this study, using the non-model ethanologenic bacterium Zymomonas mobilis as an example, we developed a workflow to mine and temper the elements of restriction-modification (R-M), CRISPR/Cas, toxin-antitoxin (T-A) systems, and native plasmids, which are hidden within industrial microorganisms themselves, as efficient genome-editing toolkits, and established a genome-wide iterative and continuous editing (GW-ICE) system for continuous genome editing with high efficiency. This research not only provides tools and pipelines for engineering the non-model polyploid industrial microorganism Z. mobilis efficiently, but also sets a paradigm to overcome biotechnological limitations in other genetically recalcitrant non-model industrial microorganisms.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production. 系统开发用于生产 5-氨基乙酰丙酸的高效细胞工厂。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-08-06 DOI: 10.1016/j.tibtech.2024.06.004

Houming Zhou, Chengyu Zhang, Zilong Li, Menglei Xia, Zhenghong Li, Zhengduo Wang, Gao-Yi Tan, Ying Luo, Lixin Zhang, Weishan Wang

{"title":"Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production.","authors":"Houming Zhou, Chengyu Zhang, Zilong Li, Menglei Xia, Zhenghong Li, Zhengduo Wang, Gao-Yi Tan, Ying Luo, Lixin Zhang, Weishan Wang","doi":"10.1016/j.tibtech.2024.06.004","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.06.004","url":null,"abstract":"The versatile applications of 5-aminolevulinic acid (5-ALA) across the fields of agriculture, livestock, and medicine necessitate a cost-efficient biomanufacturing process. In this study, we achieved the economic viability of biomanufacturing this compound through a systematic engineering framework. First, we obtained a 5-ALA synthase (ALAS) with superior performance by exploring its natural diversity with divergent evolution. Subsequently, using a genome-scale model, we identified and modified four key targets from distinct pathways in Escherichia coli, resulting in a final enhancement of 5-ALA titers up to 21.82 g/l in a 5-l bioreactor. Furthermore, recognizing that an imbalance of redox equivalents hindered further titer improvement, we developed a dynamic control system that effectively balances redox status and carbon flux. Ultimately, we collaboratively optimized the artificial redox homeostasis system at the transcription level with other cofactors at the feeding level, demonstrating the highest recorded performance to date with a titer of 63.39 g/l for the biomanufacturing of 5-ALA.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141903027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0