Trends in biotechnology最新文献_第4页

Extended pegRNAs enhance the editing capability of Prime editing. 扩展的 pegRNA 增强了 Prime 编辑的编辑能力。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-27 DOI: 10.1016/j.tibtech.2024.09.004

Kezhang He, Qiaomei Xue, Wei Zhou, Pengqi Wang, Xiaodan Hu, Tongtong Lin, Nan Chen, Bowen Wang, Tianhua Ma, Sheng Ding

{"title":"Extended pegRNAs enhance the editing capability of Prime editing.","authors":"Kezhang He, Qiaomei Xue, Wei Zhou, Pengqi Wang, Xiaodan Hu, Tongtong Lin, Nan Chen, Bowen Wang, Tianhua Ma, Sheng Ding","doi":"10.1016/j.tibtech.2024.09.004","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.09.004","url":null,"abstract":"Genome editing is highly valuable in biomedical research. Despite their versatility, current Prime editing (PE) techniques are limited to short sequence alterations [up to ~44 base pairs (bp)], and exhibit inconsistent or low efficiency across genomic loci, particularly when faced with poly-T sequences. To address these challenges, we developed an extended PE (exPE) technique that can potentially execute any precise genome editing. By harnessing RNA polymerase II (Pol II) promoters to transcribe extended PE guide RNAs (expegRNAs), exPE substantially improves editing efficiency and overcomes the challenges posed by poly-T sequences. Compared with conventional PE, exPE achieves up to a 14-fold increase in the efficiency of base conversions and short insertions, and, remarkably, up to a 259-fold improvement in regions with poly-T sequences. Uniquely, exPE enables seamless insertion of gene-sized DNA fragments into genomes, potentially correcting nearly 90% of human genetic variants, thereby broadening its applications in genetic research and therapy.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnology-enhanced genetic controls of the global pest Drosophila suzukii. 利用生物技术加强对全球害虫铃木果蝇的基因控制。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-25 DOI: 10.1016/j.tibtech.2024.09.005

Ying Yan, Hassan M M Ahmed, Ernst A Wimmer, Marc F Schetelig

引用次数: 0

Evaluating neuroprivacy concerns in human brain organoid research. 评估人脑类器官研究中的神经隐私问题。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-20 DOI: 10.1016/j.tibtech.2024.09.001

Masanori Kataoka, Shu Ishida, Chie Kobayashi, Tsung-Ling Lee, Tsutomu Sawai

引用次数: 0

Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration. 设计大规模的 hiPSC 衍生血管整合肌肉样晶格，以增强肌肉再生的体积。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-20 DOI: 10.1016/j.tibtech.2024.08.001

Myung Chul Lee, Yasamin A Jodat, Yori Endo, Alejandra Rodríguez-delaRosa, Ting Zhang, Mehran Karvar, Ziad Al Tanoury, Jacob Quint, Tom Kamperman, Kiavash Kiaee, Sofia Lara Ochoa, Kun Shi, Yike Huang, Montserrat Pineda Rosales, Adnan Arnaout, Hyeseon Lee, Jiseong Kim, Eder Luna Ceron, Isaac Garcia Reyes, Adriana C Panayi, Angel Flores Huidobro Martinez, Xichi Wang, Ki-Tae Kim, Jae-I Moon, Seung Gwa Park, Kangju Lee, Michelle A Calabrese, Shabir Hassan, Junmin Lee, Ali Tamayol, Luke Lee, Olivier Pourquié, Woo-Jin Kim, Indranil Sinha, Su Ryon Shin

{"title":"Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration.","authors":"Myung Chul Lee, Yasamin A Jodat, Yori Endo, Alejandra Rodríguez-delaRosa, Ting Zhang, Mehran Karvar, Ziad Al Tanoury, Jacob Quint, Tom Kamperman, Kiavash Kiaee, Sofia Lara Ochoa, Kun Shi, Yike Huang, Montserrat Pineda Rosales, Adnan Arnaout, Hyeseon Lee, Jiseong Kim, Eder Luna Ceron, Isaac Garcia Reyes, Adriana C Panayi, Angel Flores Huidobro Martinez, Xichi Wang, Ki-Tae Kim, Jae-I Moon, Seung Gwa Park, Kangju Lee, Michelle A Calabrese, Shabir Hassan, Junmin Lee, Ali Tamayol, Luke Lee, Olivier Pourquié, Woo-Jin Kim, Indranil Sinha, Su Ryon Shin","doi":"10.1016/j.tibtech.2024.08.001","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.08.001","url":null,"abstract":"Engineering biomimetic tissue implants with human induced pluripotent stem cells (hiPSCs) holds promise for repairing volumetric tissue loss. However, these implants face challenges in regenerative capability, survival, and geometric scalability at large-scale injury sites. Here, we present scalable vessel-integrated muscle-like lattices (VMLs), containing dense and aligned hiPSC-derived myofibers alongside passively perfusable vessel-like microchannels inside an endomysium-like supporting matrix using an embedded multimaterial bioprinting technology. The contractile and millimeter-long myofibers are created in mechanically tailored and nanofibrous extracellular matrix-based hydrogels. Incorporating vessel-like lattice enhances myofiber maturation in vitro and guides host vessel invasion in vivo, improving implant integration. Consequently, we demonstrate successful de novo muscle formation and muscle function restoration through a combinatorial effect between improved graft-host integration and its increased release of paracrine factors within volumetric muscle loss injury models. The proposed modular bioprinting technology enables scaling up to centimeter-sized prevascularized hiPSC-derived muscle tissues with custom geometries for next-generation muscle regenerative therapies.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bringing carbon to life via one-carbon metabolism. 通过一碳新陈代谢让碳焕发生机。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-20 DOI: 10.1016/j.tibtech.2024.08.014

Samantha O'Keeffe, Lilly Garcia, Yi Chen, Richard C Law, Chong Liu, Junyoung O Park

引用次数: 0

Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia. 利用红树林土壤微生物组来选择聚对苯二甲酸乙二醇酯转化细菌联合体。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-20 DOI: 10.1016/j.tibtech.2024.08.013

Diego Javier Jiménez, Dayanne Chaparro, Felipe Sierra, Gordon F Custer, Golo Feuerriegel, Maria Chuvochina, Laura Diaz-Garcia, Lucas William Mendes, Yina Paola Ortega Santiago, Carolina Rubiano-Labrador, Felipe Salcedo Galan, Wolfgang R Streit, Francisco Dini-Andreote, Alejandro Reyes, Alexandre Soares Rosado

{"title":"Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia.","authors":"Diego Javier Jiménez, Dayanne Chaparro, Felipe Sierra, Gordon F Custer, Golo Feuerriegel, Maria Chuvochina, Laura Diaz-Garcia, Lucas William Mendes, Yina Paola Ortega Santiago, Carolina Rubiano-Labrador, Felipe Salcedo Galan, Wolfgang R Streit, Francisco Dini-Andreote, Alejandro Reyes, Alexandre Soares Rosado","doi":"10.1016/j.tibtech.2024.08.013","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.08.013","url":null,"abstract":"Mangroves are impacted by multiple environmental stressors, including sea level rise, erosion, and plastic pollution. Thus, mangrove soil may be an excellent source of as yet unknown plastic-transforming microorganisms. Here, we assess the impact of polyethylene terephthalate (PET) particles and seawater intrusion on the mangrove soil microbiome and report an enrichment culture experiment to artificially select PET-transforming microbial consortia. The analysis of metagenome-assembled genomes of two bacterial consortia revealed that PET catabolism can be performed by multiple taxa, of which particular species harbored putative novel PET-active hydrolases. A key member of these consortia (Mangrovimarina plasticivorans gen. nov., sp. nov.) was found to contain two genes encoding monohydroxyethyl terephthalate hydrolases. This study provides insights into the development of strategies for harnessing soil microbiomes, thereby advancing our understanding of the ecology and enzymology involved in microbial-mediated PET transformations in marine-associated systems.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial design of the genome: from sequences to the 3D structure of chromosomes. 基因组的人工设计：从序列到染色体的三维结构。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-18 DOI: 10.1016/j.tibtech.2024.08.012

Jun-Yi Wang, Ze-Xiong Xie, You-Zhi Cui, Bing-Zhi Li, Ying-Jin Yuan

引用次数: 0

Protein nanocarrier-enabled plant genetic engineering systems. 蛋白质纳米载体植物基因工程系统。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-13 DOI: 10.1016/j.tibtech.2024.08.009

Temoor Ahmed, Muhammad Noman, Yetong Qi, Jason C White, Xingjiang Qi

引用次数: 0

Semi-continuous biomanufacturing for maximizing the production of complex chemicals and fuels: a case study of amorpha-4,11-diene. 最大限度地生产复杂化学品和燃料的半连续生物制造技术：α-4,11-二烯案例研究。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-13 DOI: 10.1016/j.tibtech.2024.08.010

Carlos Castillo-Saldarriaga, Stephen Sarria, Christine N S Santos, Parayil K Ajikumar, Ralf Takors

{"title":"Semi-continuous biomanufacturing for maximizing the production of complex chemicals and fuels: a case study of amorpha-4,11-diene.","authors":"Carlos Castillo-Saldarriaga, Stephen Sarria, Christine N S Santos, Parayil K Ajikumar, Ralf Takors","doi":"10.1016/j.tibtech.2024.08.010","DOIUrl":"https://doi.org/10.1016/j.tibtech.2024.08.010","url":null,"abstract":"Biomanufacturing is emerging as a key technology for the sustainable production of chemicals, materials, and food ingredients using engineered microbes. However, despite billions of dollars of investment, few processes have been successfully commercialized due to a lack of attention on industrial-scale bioprocess design and innovation. In this study, we address this challenge through the development of a novel semi-continuous bioprocess for the production of the terpene amorpha-4,11-diene (AMD4,11) using engineered Escherichia coli. Using a hydrophilic membrane for product and biomass retention, we successfully decoupled production at low growth rates (~0.01 1/h) and improved reactor productivity up to 166 mg/lReactor h, threefold compared with traditional fed-batch fermentations. When cell recycling was implemented, we showed sustained production at the highest conversion yield and production rate for up to three cycles, demonstrating the robustness of both the strain and the process and highlighting the potential for new bioprocess strategies to improve the economic viability of industrial biomanufacturing.","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":null,"pages":null},"PeriodicalIF":14.3,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards more realistic cultivated meat by rethinking bioengineering approaches. 通过重新思考生物工程方法，实现更逼真的人工肉类。

IF 14.3 1区工程技术

Trends in biotechnology Pub Date : 2024-09-12 DOI: 10.1016/j.tibtech.2024.08.008

Marie Piantino, Quentin Muller, Chika Nakadozono, Asuka Yamada, Michiya Matsusaki

引用次数: 0