Tissue engineering. Part C, Methods最新文献

{"title":"Development of a Complex Human <i>In Vitro</i> Model of Endochondral Ossification.","authors":"Encheng Ji, Amaia Garmendia Urdalleta, Janneke Witte-Bouma, Gert-Jan Kremers, Nunzia Di Maggio, Andrea Banfi, Eric Farrell, Andrea Lolli","doi":"10.1177/19373341251378152","DOIUrl":"10.1177/19373341251378152","url":null,"abstract":"<p><p>During development and regeneration, bone is formed by endochondral ossification (EO) through the remodeling of a cartilage template. This complex process involves multiple cell types and interactions that cannot currently be modeled <i>in vitro</i>. This study aimed to develop a novel tissue-engineered human <i>in vitro</i> model of certain aspects of the early stages of EO by integrating cartilage which undergoes mineralization, self-assembled vascular networks, and osteoclasts into a single system. We first studied the dynamics of osteoclastogenesis and vascularization in an <i>in vivo</i> model of stromal cell-mediated EO, to inform our <i>in vitro</i> system. Next, we aimed to develop a fully human cell-based three-dimensional model of EO by combining pediatric bone marrow stromal cells differentiating into chondrocytes, osteoclasts derived from human CD14+ monocytes, and human umbilical vein endothelial cells and adipose-derived stromal cells as vessel-forming cells. We investigated how mineralizing cartilage affects osteoclast and vessel formation <i>in vitro</i> through separate cartilage-osteoclasts and cartilage-vessels cocultures. Finally, we combined these elements and established a complex <i>in vitro</i> model that supports the functionality of all these cell types and recapitulates chondrogenesis, cartilage mineralization, vessel formation and osteoclastogenesis. This integrated approach reaches unprecedented complexity and will enable new tissue engineering strategies to model skeletal diseases or cancer metastasis to the bone.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"105-120"},"PeriodicalIF":2.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Stiffness Hydrogel-Based Glioblastoma <i>In Vitro</i> Model to Observe Cell Behavior at Interfaces.","authors":"Allison Faber, Shabnam Nejat, James Baker, Silviya P Zustiak","doi":"10.1177/19373384261438217","DOIUrl":"10.1177/19373384261438217","url":null,"abstract":"<p><p>There is renewed interest in three-dimensional <i>in vitro</i> bioengineered models that replicate key aspects of the <i>in vivo</i> environment for the study of cellular behavior, with one key aspect being cell interactions with matrix interfaces. Here, we developed a dual-stiffness hydrogel-encapsulated glioblastoma (GBM) spheroid model to investigate GBM spreading along a stiffness interface. GBM is an aggressive brain cancer with a patient prognosis of 12-18 months, which is known to spread to distant brain regions by following stiffness interfaces. Our model consisted of a soft, 5% w/v, polyethylene glycol (PEG) hydrogel to mimic the native brain tissue and a stiff, 10% w/v, PEG hydrogel to replicate the stiffer GBM microenvironment. To ensure spheroids fall along the boundary, we adjusted the gelation time of the gel by varying the pH of the gel precursor solution. Encapsulated spheroids were assessed for infiltration and viability for up to 7 days. Spheroids exhibited high viability in all hydrogels. Spheroids showed a higher infiltration index in the soft hydrogel, and migration across the stiffness interface occurred only from the soft to the stiff hydrogel in the dual-stiffness gels. The developed model has a simple, robust design for studying GBM behavior <i>in vitro</i>, a high degree of imageability, requires no specialized equipment to prepare, and is compatible with a multiwell plate format for easy handling and analysis.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"138-150"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue on Cancer and Regenerative Medicine.","authors":"Stephanie K Seidlits, Jeroen J J P van den Beucken","doi":"10.1177/19373384261438773","DOIUrl":"10.1177/19373384261438773","url":null,"abstract":"","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"103-104"},"PeriodicalIF":2.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147634306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-Cost, Rapid Fabrication of Customizable Polyethylene Glycol-Based Cell Culture Devices.","authors":"Emily L Pallack, Maxwell W Oulundsen, Yan Kolpakov, Hannah R Goldberg, Aneth J Fernandez, Noah D Teaney, Faith E Y Moran, Nisha R Iyer","doi":"10.1177/19373384261427525","DOIUrl":"10.1177/19373384261427525","url":null,"abstract":"<p><p>Biological research groups may face a high barrier to entry when constructing custom 3D cell culture devices to investigate multi-tissue interactions <i>in vitro</i>. Standard fabrication methods such as lithography, etching, or molding are expensive and require specialized equipment and expertise. To address this, we developed an accessible approach for producing polyethylene glycol (PEG)-based cell culture devices using stereolithography 3D printing with a polydimethylsiloxane intermediate mold. Both the intermediate molding steps and the sterilized final device show low cytotoxicity, and the final device swells to predictable dimensions and retains its shape for at least 10 days. We used this approach to develop a human pluripotent stem cell-derived neural spheroid outgrowth model that supports directed neurite extension over 14 days. Together, this method provides a highly customizable, affordable platform for rapid fabrication of PEG-based microphysiological systems for diverse tissue engineering applications.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"80-89"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147435645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated Seamless Poly(ε-Caprolactone) Electrospun Tubes for Critically-Sized Bone Defect Repair.","authors":"Kelly L O'Neill, Kylie E Williams, Auveen Hajarizadeh, Adam Rauff, Ievgenii Liashenko, Robert E Guldberg, Paul D Dalton","doi":"10.1177/19373368261431109","DOIUrl":"10.1177/19373368261431109","url":null,"abstract":"<p><p>In this study, we demonstrate an automated approach to efficiently and reproducibly manufacture perforated poly(ε-caprolactone) (PCL) solution electrospun tubular meshes designed for critically-sized bone defect repair. The workflow improves reproducibility and reduces fabrication time by 67% (8.7 vs. 2.7 h per 10 meshes). By directly electrospinning PCL onto a rotating cylindrical mandrel, seam-related discontinuities are eliminated, and subsequent use of an automated soldering iron system enables precise 1 mm perforations that promote vascular ingrowth during bone healing. Despite the decrease in mass of the new design compared with the original design (18.24 ± 1.5 mg for old vs. 11.48 ± 1.2 mg for new design), mechanical testing revealed similar resistance to lateral compression compared with semimanually assembled meshes. This is important to prevent collapse during surgical placement and injection of osteoinductive treatments. Further, eliminating surgical glue improves the manufacturing simplicity and scaffold reproducibility. Following implantation with bone morphogenic protein-2 loaded alginate, the new design performed similarly to the original: <i>in vivo</i> microcomputed tomography confirmed bone formation that significantly increased (<i>p</i> ≤ 0.05) over 8-weeks in an established rat femoral defect model. This study provides a novel production method of tubular scaffolds with variable dimensions and flexible perforation patterns and demonstrates improvements in fabrication efficiencies and reproducibility.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"71-79"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147460066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An <i>Ex Vivo</i> Mini-Tumor Culture Protocol for Evaluating Individualized Efficacy of Chemotherapy and Immunotherapy.","authors":"Yuanfeng Fu, Yuxia Zou, Zhilong Chen, Chenglong Zheng, Dongmeng Liu, Tingting Duan, Jinlong Huang, Xuejun Zhou, Jiaolin Bao, Xiaofeng Wang, Ren-Bo Ding","doi":"10.1177/19373384261431824","DOIUrl":"10.1177/19373384261431824","url":null,"abstract":"<p><p>Conventional drug screening models face a series of challenges in guiding individualized cancer treatment, including time-consuming processes, limited drug coverage, and insufficient accuracy in efficacy evaluation. This study aims to establish a convenient, rapid, and reliable drug screening protocol for evaluating individualized efficacy of chemotherapy and immunotherapy. We established an <i>ex vivo</i> mini-tumor culture platform by culturing tumor fragments in an air-liquid interface system, which was capable of sustaining tumor growth for at least 2 weeks and maintaining immune cell infiltration for over 1 week. Using this mini-tumor culture platform, we can evaluate the individualized therapeutic responses of different tumors to chemotherapy and immunotherapy, including gemcitabine, 5-fluorouracil, cisplatin, αPD-1 and αPD-L1. Furthermore, using this drug evaluation platform, we revealed distinct therapeutic responses to immunotherapy between immune-cold tumors and immune-hot tumors, and demonstrated the important role of the immunologic adjuvant resiquimod (R848) in enhancing immunotherapy efficacy. This mini-tumor culture protocol provides a feasible implementation approach for <i>ex vivo</i> personalized drug testing.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"90-98"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147435717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evolving Landscape of Colorectal Organoids in Tissue Engineering: Research Trends and Hotspots.","authors":"Huiping Gan, Pan Huang, Jia Wang, Lei Gao, Tao Li","doi":"10.1177/19373384261417822","DOIUrl":"10.1177/19373384261417822","url":null,"abstract":"<p><p>Colorectal organoids, which accurately replicate the structure and function of the human colorectal epithelium, have become a valuable platform in a broad spectrum of fundamental biological research and clinical applications. This study employs bibliometric analysis to develop a knowledge domain map specifically focusing on colorectal organoid research. Articles were sourced from the Web of Science Core Collection, and CiteSpace 6.3.R1 was utilized to analyze the literature, including outputs, journals, countries, institutions, authors, cocited authors, references, co-occurring terms, and burst terms. Subsequently, we examined prevailing research themes and focal points and identified potential future research directions within this domain. Between 2010 and 2025, a total of 719 articles related to colorectal organoid research were published. Among these, the journal <i>Nature Communications</i> published the highest number of papers. The United States and Utrecht University were identified as the most prolific country and institution, respectively. Hans Clevers emerged as the most prolific author, while Toshiro Sato had the highest number of cocitations, indicating that both are ideal candidates for academic collaboration. The research focus on colorectal organoids has evolved from basic biological characteristics to disease modeling and clinical applications, and further towards an in-depth exploration of functional mechanisms and precision medicines. The terms \"patient-derived organoids\", \"disease modeling\", \"epithelial barrier\", and \"personalized medicine\" have garnered significant attention between 2020 and 2025, highlighting them as promising areas for future research. Research on colorectal organoids has achieved substantial progress, positioning itself as a vital interdisciplinary field that integrates fundamental biology with clinical medicine. Future studies should focus on optimizing organoid culture methodologies, exploring functional mechanisms, and expanding clinical applications-especially in disease modeling and personalized medicine.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"43-55"},"PeriodicalIF":2.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Perfusion Imaging of Microcirculatory Networks Within Axially Vascularized Artificial Tissue.","authors":"Christoph Koepple, Lukas Pollmann, Nicola Sariye Pollmann, Ulrich Kneser, Dirk J Schaefer, Norbert Gretz, Volker J Schmidt","doi":"10.1177/19373384261422709","DOIUrl":"10.1177/19373384261422709","url":null,"abstract":"<p><p>Evaluating the complex, three-dimensional (3D) architecture of <i>de novo</i> angiogenesis in artificially engineered tissue remains a significant challenge, as conventional methods like 2D histology and microimaging techniques are limited. For axial vascularization techniques, a reproducible method for complete visualization of the microcirculatory system is needed. We present an integrated workflow for high-resolution 3D visualization of neovascularization within arteriovenous (AV) loop-based tissue constructs in a rat model. An intravascular perfusion with a cationic near-infrared fluorescent dye, MHI148-polyethylenimine, was used to 3D label the patent vasculature. Following perfusion-fixation and explantation, the construct was rendered optically transparent using an ethyl cinnamate-based clearing protocol. The fluorescent signal was then imaged using confocal and light-sheet fluorescence microscopy at 7 and 28 days postimplantation. Our workflow successfully achieved high-contrast, 3D visualization of the microvascular network, allowing for whole-mount and segmental analysis of the vascular tree. At day 7, imaging delineated solely the AV loop axis while by day 28, a dense and complex, interconnected capillary plexus from the central axis demonstrated a progressive neovascularization. Downstream processing compatibility was confirmed through successful rehydration and 3D nuclear counterstaining. This workflow offers a powerful and reproducible method for detailed structural assessment of microvascular networks in large engineered constructs, overcoming key limitations of existing techniques.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"56-65"},"PeriodicalIF":2.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146158472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> MSC Characterization of Cryopreserved Adipose Stromal Cells Processed via Uvence Workflow.","authors":"Ahsan A Khan, Olivier D Amar, Sumila Rathnayaka M, Chloe Dumoulin, Jeremy Magalon, John Packer, Abbas Ishaq, Anne M Dickinson","doi":"10.1177/19373384251413859","DOIUrl":"10.1177/19373384251413859","url":null,"abstract":"<p><p>Adipose tissue is an abundant and clinically accessible source of stromal cells. Stromal vascular fraction (SVF) and nanofat have been widely investigated for their regenerative potential; however, commercial systems vary considerably in yield, viability, and regulatory oversight. Most devices report fresh results only, with limited validation following cryopreservation. Mesenchymal stromal cells derived from adipose tissue have also attracted attention due to their accessibility, immunomodulatory effects, and multipotent differentiation capacity. Uvence has developed a proprietary workflow for adipose tissue processing that integrates washing, cryopreservation, thawing, and emulsification within a Human Tissue Authority-regulated laboratory. The process includes Good Manufacturing Practices (GMP) Annex 1-aligned environmental monitoring and independent quality control (QC) testing. Critically, this workflow validates postthaw cell viability, addressing a gap in current SVF/nanofat approaches. Three cryopreserved donor samples demonstrated a mean postthaw viability of ∼91% (range 90.5-92%), consistently exceeding the International Federation for Adipose Therapeutics and Science (IFATS)/ International Society for Cell and Gene Therapy (ISCT) 70% threshold. Benchmarking against global systems showed Uvence postthaw viability to be equivalent to or higher than fresh outcomes reported for enzymatic platforms (Celution, 85-91%; InGeneron, 86%) and mechanical platforms (Lipocube, Tulip, ∼96%). Unlike competitor devices, Uvence has validated freeze-thaw performance, providing a stable and compliant platform. This study also presents <i>in vitro</i> culture and characterization of stromal cells expanded from Uvence nanofat-derived SVF samples, including flow cytometry, morphology, and trilineage differentiation. Flow cytometry confirmed high expression of CD73, CD90, and CD105, with minimal expression of CD34/CD45, consistent with the ISCT criteria. While these findings are limited to research characterization and do not constitute approval for therapeutic use, they demonstrate that the Uvence workflow delivers a quality-focused approach to adipose tissue processing.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"32 1","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146107332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Safety and Efficacy of a Novel Alginate-Based Esophageal Mucosal Wound Protective Gel.","authors":"Han Wang, Jiaqi Li, Minyi Yang, Xiaomeng Su, Fanshan Qiu, Wei Huang, Jianying Dai, Qianqian Han","doi":"10.1177/19373384251406084","DOIUrl":"10.1177/19373384251406084","url":null,"abstract":"<p><strong>Background & objective: </strong>Endoscopic Submucosal Dissection (ESD) effectively treats early gastric cancer, but postoperative complications limit its clinical use. Therefore, this study examines how esophageal mucosal wound protective gels improve wound healing and reduce post-ESD complications.</p><p><strong>Methods: </strong>The gels were characterized for physical properties and stability using rheological behavior, injectability, swelling capacity, and enzymatic degradation resistance. Biocompatibility was assessed via hemolysis testing, cytotoxicity assays, and oral mucosal irritation tests. Furthermore, wound repair potential was evaluated through cell proliferation, migration, and cell cycle analysis in Het-1A cells. Finally, in vivo recovery experiments were conducted to assess post-ESD wound healing efficacy.</p><p><strong>Results: </strong>The gels exhibited favorable physical properties, chemical stability, and biocompatibility. Specifically, they maintained stability in the digestive tract, underwent rapid gelation at 37°C, and promoted cell proliferation. Post-ESD evaluation further revealed improved mucosal healing with no significant bleeding events.</p><p><strong>Conclusion: </strong>The developed esophageal mucosal wound-protective gels fulfill the requirements for submucosal interventions and show promising potential for ESD wound repair via rapid in situ gelation. This platform could be adapted for various endoscopic procedures and provides new insights for digestive tract tissue engineering applications.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"32 1","pages":"24-34"},"PeriodicalIF":2.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146107375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}