Tissue engineering. Part C, Methods最新文献_第2页

Rosalind Franklin Society Proudly Announces the 2024 Award Recipient for Tissue Engineering: Part C. 罗莎琳德·富兰克林协会自豪地宣布2024年组织工程奖获得者：C部分。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-04-01 DOI: 10.1089/ten.tec.2023.0289.rfs2024

Zhanpeng Xu

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Correction to: Xenogeneic Decellularized Scaffold: A Novel Platform for Ovary Regeneration doi: 10.1089/ten.tec.2016.0410. 异种脱细胞支架：卵巢再生的新平台doi: 10.1089/ten.tec.2016.0410。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-04-01 DOI: 10.1089/ten.tec.2016.0410.correx

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Editorial: The Need for Validation of Organoids in Regenerative Medicine Research. 社论：在再生医学研究中验证类器官的必要性。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-04-01 DOI: 10.1089/ten.tec.2025.15482

John A Jansen

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An Abbreviated Rabbit Knee Model of Joint Contracture. 兔膝关节挛缩模型的建立。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-04-01 DOI: 10.1089/ten.tec.2025.0044

Kareme D Alder, Mason F Carstens, Cole E Bothun, Oliver B Dilger, Ashley N Payne, Roman Thaler, Mark E Morrey, Joaquin Sanchez-Sotelo, Daniel J Berry, Amel Dudakovic, Matthew P Abdel

{"title":"An Abbreviated Rabbit Knee Model of Joint Contracture.","authors":"Kareme D Alder, Mason F Carstens, Cole E Bothun, Oliver B Dilger, Ashley N Payne, Roman Thaler, Mark E Morrey, Joaquin Sanchez-Sotelo, Daniel J Berry, Amel Dudakovic, Matthew P Abdel","doi":"10.1089/ten.tec.2025.0044","DOIUrl":"https://doi.org/10.1089/ten.tec.2025.0044","url":null,"abstract":"Experimental analyses of knee joint contractures have traditionally utilized a 6-month rabbit model as the gold standard. However, this model is time-intensive and costly. The purpose of this study was to develop an abbreviated rabbit model of knee contractures and compare it to the well-established longer model. Twenty female New Zealand White rabbits were divided into two equal groups and prospectively studied to assess knee passive extension angles (PEA), contracture angles (CA), and terminal posterior capsular stiffness. Experimental knees were immobilized for either 4 weeks (n = 10) with an 8-week remobilization period in the abbreviated model (i.e., 3 months) or for 8 weeks (n = 10) with a 16-week remobilization period in the standard model (i.e., 6 months). PEAs were assessed at remobilization and several time points using differing vertical forces. At sacrifice, terminal biomechanical data were collected to assess posterior capsular stiffness. Analysis of PEAs in live animals at each torque value and time point demonstrated increased PEAs and decreased CAs in the 3-month abbreviated model as compared to the 6-month standard model. At sacrifice, biomechanical analysis demonstrated that the posterior capsules of the 3-month experimental limbs were significantly more stiff than the contralateral limb (2.4 vs. 0.05 Ncm/°, p < 0.0001), but significantly less stiff compared to the 6-month experimental limbs (2.4 vs. 4.7 Ncm/°, p < 0.0001). Our study suggests that the 6-month standard rabbit knee model of arthrofibrosis should continue to be used in the laboratory assessment of arthrofibrosis. However, the abbreviated model may be beneficial under selected experimental conditions.","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 4","pages":"145-152"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Natural Killer Cell Membrane Isolation with Minimal Nuclear, Mitochondrial, and Cytosol Contamination. 自然杀伤细胞膜分离与最小的核，线粒体，和细胞质污染。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-04-01 DOI: 10.1089/ten.tec.2024.0362

Vaishali Chugh, Vijaya Krishna K, Vaibhav Patil, Abhay Pandit

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Comparative Insights into Bone Substitutes for Two-Stage Maxillary Sinus Floor Elevation: A Bayesian Network Approach. 两阶段上颌窦底抬高骨替代物的比较研究：贝叶斯网络方法。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-03-01 Epub Date: 2025-03-10 DOI: 10.1089/ten.tec.2025.0010

Jiayi Chen

{"title":"Comparative Insights into Bone Substitutes for Two-Stage Maxillary Sinus Floor Elevation: A Bayesian Network Approach.","authors":"Jiayi Chen","doi":"10.1089/ten.tec.2025.0010","DOIUrl":"10.1089/ten.tec.2025.0010","url":null,"abstract":"To investigate the histomorphometric performance of two-stage maxillary sinus floor elevation (TMSFE) with various bone substitutes in the treatment of atrophic posterior maxilla. Four databases (PubMed, Embase, Web of Science, and The Cochrane Library) were searched from the beginning of database establishment to August 8, 2023. The included articles were limited to the English language. A systematic search was performed to identify randomized controlled trials assessing the histological performance of various biomaterials in TMSFE with a follow-up of 5-8 months. The main outcome was an area of new bone, and an additional outcome was residual graft material. Extracted data were analyzed by using a Bayesian approach (the Markov chain Monte Carlo) to establish ranks of various biomaterials in R language. Finally, the search identified 22 studies that reported 22 trials on bone area (17 kinds of biomaterials) and 12 studies on residual graft materials (12 kinds of biomaterials) after the exclusion of one study disconnected from the network plot. No local inconsistency could be found in studies regarding bone formation, while no closed loop was detected in residual graft material. The top 3 probabilities of biomaterials in terms of bone formation were Allograft + Xenograft (AG + X) (87.14%), X + Polymer (75.69%), and Autogenous Bone + Bioactive Glass (AB + BG) (71.44%). AG + X had the highest probability (87.14%) of being the most optimal treatment for bone formation. Biphasic calcium phosphate + Fibrin sealant (BCP + FS) was ranked as the slowest absorbing biomaterial (78.27%) in TMSFE. Within the limitations of the current network meta-analysis, AG + X may represent an optimal biomaterial for bone formation in TMSFE. The use of X in combination with other biomaterials demonstrates superior osteogenic effects in TMSFE. BCP + FS exhibited strong mechanical properties during a short-term observational period. The present findings suggest that AB is not the only feasible standard for bone grafts.","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"130-141"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Spontaneous Spheroids of hUC-MSCs Regulate Osteogenic Differentiation for Enhancing Osteogenesis. hUC-MSCs自发球体调节成骨分化促进成骨。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-03-01 Epub Date: 2025-03-10 DOI: 10.1089/ten.tec.2024.0297

Xiaotong Wei, Xianqi Li, Zhu Wen, Naoto Oguchi, Jing Yang, Hideaki Kagami, Yuji Kurihara

{"title":"Spontaneous Spheroids of hUC-MSCs Regulate Osteogenic Differentiation for Enhancing Osteogenesis.","authors":"Xiaotong Wei, Xianqi Li, Zhu Wen, Naoto Oguchi, Jing Yang, Hideaki Kagami, Yuji Kurihara","doi":"10.1089/ten.tec.2024.0297","DOIUrl":"10.1089/ten.tec.2024.0297","url":null,"abstract":"Stem cells play a critical role in the regeneration process by proliferating and differentiating to form new bone tissue. However, stem cells tend to lose their stemness and pluripotency during in vitro expansion, resulting in reduced bone regeneration capacity after osteogenic induction. Our aim is to enhance the osteogenic impact of human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) through spontaneous spheroid in vitro. The pluripotency and osteogenesis-related genes up-regulated in hUC-MSCs can be enhanced in spontaneous spheroids in vitro. For in vivo testing, spontaneous spheroids were transplanted into mice using beta-tricalcium phosphate as a scaffold. Transplant samples were stained using hematoxylin and eosin (HE), immunohistochemistry, and TRAP staining. The samples showed new bone formation, upregulated SP7 and OCN expression, and more vigorous bone metabolism in the Sph-OI group than the other groups. However, new bone formation was mainly immature bone. Overall, our findings demonstrate that hUC-MSC spheroids possess remarkable pluripotency, with the spontaneous spheroids formed following osteogenic induction exhibiting enhanced osteogenic differentiation potential and bone regeneration capacity. However, optimizing the osteogenic differentiation process and elucidating the underlying mechanisms of bone regeneration are critical scientific issues that urgently need to be addressed to enable its application in bone regeneration.","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"108-118"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Progress of Pyruvate Kinase M2 in Hepatocellular Carcinoma-Associated Signaling Pathway. 丙酮酸激酶M2在肝细胞癌相关信号通路中的研究进展。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-03-01 DOI: 10.1089/ten.tec.2024.0368

Qi Wan, Chunlian Zhao, Rui Zhao

{"title":"Progress of Pyruvate Kinase M2 in Hepatocellular Carcinoma-Associated Signaling Pathway.","authors":"Qi Wan, Chunlian Zhao, Rui Zhao","doi":"10.1089/ten.tec.2024.0368","DOIUrl":"10.1089/ten.tec.2024.0368","url":null,"abstract":"Hepatocellular carcinoma (HCC) is an aggressive liver tumor with a unique metabolic profile and a shift to glycolytic metabolism. This review discusses the contribution of pyruvate kinase M2 (PKM2) to HCC development and its potential as a target for therapy. We carried out a broad literature review on PKM2, focusing on its role in the glycolytic pathway and special interactions with key signaling pathways like Phosphoinositide 3-kinase/Protein kinase B/Mammalian target of rapamycin (PI3K/AKT/mTOR) and Mitogen-activated protein kinase (MAPK). PKM2 also performs a dual role in energy metabolism and signal transduction in HCC. PKM2 is paramount in the induction of HCC by regulating cellular metabolism and oncogenic signaling pathways. It promotes tumor growth, survival, and metastasis through interaction with the PI3K/AKT/mTOR and MAPK pathways. PKM2 is a key factor in HCC pathogenesis, with a dual impact on metabolism and signaling. Its properties may open the way for developing novel therapeutic interventions against HCC. Thus, PKM2 inhibition may offer further opportunities for tumor growth blockade, which could meaningfully improve patients' clinical outcomes.","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 3","pages":"101-107"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Mice Models for Peripheral Denervation to Enhance Vascular Regeneration. 外周去神经支配增强血管再生的小鼠模型。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-03-01 Epub Date: 2025-03-10 DOI: 10.1089/ten.tec.2025.0014

Taylor K Brown, Caitlyn Dang, Aurea Del Carmen, Sara Alharbi, Calvin L Chao, Liqun Xiong, Nikita Wilson John, Aidan Smires, Karen J Ho, Bin Jiang

{"title":"Mice Models for Peripheral Denervation to Enhance Vascular Regeneration.","authors":"Taylor K Brown, Caitlyn Dang, Aurea Del Carmen, Sara Alharbi, Calvin L Chao, Liqun Xiong, Nikita Wilson John, Aidan Smires, Karen J Ho, Bin Jiang","doi":"10.1089/ten.tec.2025.0014","DOIUrl":"10.1089/ten.tec.2025.0014","url":null,"abstract":"Sympathetic innervation plays a critical role in regulating vascular function, yet its influence on vascular regeneration and reinnervation following ischemic injury remains poorly understood. This study develops and validates murine models of localized sympathetic denervation using 6-hydroxydopamine (6-OHDA) to enable study of the sympathetic nervous system's impact on vascular systems during tissue repair. Two methods of 6-OHDA administration were employed: a single topical application during open surgery and minimally invasive weekly subcutaneous injections. The topical application model achieved temporary denervation lasting 1 week without causing vascular damage, while the subcutaneous injection model provided sustained denervation for up to 4 weeks with minimal inflammation and no significant changes to vascular architecture. To investigate the effects of denervation in an ischemic context, these models were combined with a hindlimb ischemia model. Ischemia induced persistent denervation in both 6-OHDA-treated and control limbs, with limited sympathetic nerve regeneration observed over 4 weeks. Despite persistent denervation, microvascular density and perfusion recovery in ischemic muscles were comparable between denervated and control groups. This suggests that ischemia governs vascular regeneration independently of sympathetic input. These results demonstrate that localized 6-OHDA administration provides a versatile tool for achieving controlled sympathetic denervation in peripheral arteries. These models provide a novel platform for studying vascular regeneration and reinnervation under both normal and ischemic conditions, offering novel insights into the interactions between neural regulation and vascular repair processes. This work lays the foundation for future research into neural-vascular crosstalk and new possibilities for developing regenerative therapies targeting the autonomic regulation of vascular health.","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"119-129"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Use of HuEpiderm for Evaluating the Effectiveness of Biomaterials in Skin Repair. 利用hu表皮评价生物材料在皮肤修复中的有效性。

IF 2.7 4区医学

Tissue engineering. Part C, Methods Pub Date : 2025-02-01 Epub Date: 2025-01-31 DOI: 10.1089/ten.tec.2024.0298

Fanshan Qiu, Hang Zeng, Xiaomeng Su, Han Wang, Qianqian Han

{"title":"The Use of HuEpiderm for Evaluating the Effectiveness of Biomaterials in Skin Repair.","authors":"Fanshan Qiu, Hang Zeng, Xiaomeng Su, Han Wang, Qianqian Han","doi":"10.1089/ten.tec.2024.0298","DOIUrl":"10.1089/ten.tec.2024.0298","url":null,"abstract":"In vitro experiments, a crucial component of preclinical research, are widely used due to their accessibility and controlled conditions. However, traditional two-dimensional (2D) cell models are limited in their ability to simulate the complex interactions in organ systems. To address it, emerging technologies have shifted cell cultures from 2D to three-dimensional (3D), offering improved in vitro-in vivo correlation for traditional in vitro screening. Reconstructed human epidermis (RHE) is a 3D skin tissue model that closely mimics human skin in both structure and function. We established a sodium dodecyl sulfate (SDS)-induced epidermal injury model on RHE, and the result demonstrated that treating RHE with a 2.5 mg/mL SDS solution for 24 h could cause a significant epidermal damage. We also treated it with common clinical repair biomaterials, to screen the key indicator of SDS-induced 3D epidermal injury model, which includes several chemokines such as regulated upon activation normal T-cell expressed and secreted and interferon-γ-induced protein 10 that triggered inflammatory responses, and the important component protein of barrier structure-filaggrin and loricrin. In this study, we provide a platform for biomaterials evaluation that offers support and complementarities for in vitro experiments of skin repair.","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"65-73"},"PeriodicalIF":2.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0