Tissue engineering. Part C, Methods最新文献

{"title":"Clinical Prediction Models in Elderly Acute Coronary Syndrome: A Bibliometric Analysis of Research Hotspots.","authors":"Yu Wan, Lu Xiao, Xiang Zhan","doi":"10.1177/19373384261494750","DOIUrl":"https://doi.org/10.1177/19373384261494750","url":null,"abstract":"<p><p>Rapid global aging has intensified the cardiovascular disease burden, making clinical prediction models (CPMs) crucial for risk stratification and management of elderly patients with acute coronary syndrome (ACS), yet the knowledge structure of this field remains insufficiently characterized. Literature published up to November 14, 2025, was retrieved from the Web of Science Core Collection, and Bibliometrix, VOSviewer, and CiteSpace were used to analyze annual publication trends, core contributors, collaboration networks, cocited references, and keyword clusters. A total of 3,899 articles were included. From 2007 to 2025, annual publications increased from 98 to 281, with an average annual growth rate of 6.03%, peaking at 303 articles in 2022. The United States was the most productive country (1,419 articles), Harvard Medical School the leading institution (130 articles), and Krumholz HM the most prolific author (52 articles). Research hotspots concentrated on mortality and prognostic risk prediction, models associated with clinical interventions (particularly those related to percutaneous coronary intervention), and machine learning applications; since 2018, the research frontier has evolved toward intelligent technologies. Research on CPMs in elderly ACS thus shows stable and continuous growth, with a shift from traditional statistical methods toward intelligent and individualized approaches. Future priorities include externally validated models incorporating geriatric-specific variables, multicenter validation in real-world settings, and standardized comparisons of modeling approaches. The mapped evidence also informs regenerative cardiology, in which validated prediction models are needed to select elderly patients for emerging tissue engineering and regenerative interventions. Bibliometric mapping characterizes publication patterns and knowledge structures but cannot by itself establish model quality or clinical benefit.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"19373384261494750"},"PeriodicalIF":2.0,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Bibliometric Atlas of Vascular Stent Research (2015-2025): from Passive Stents to Bioactive Intelligence.","authors":"Qingqiang Cheng, Yuhang Wei, Wenjing Zhou, Enrong Chen, Yuyang Chen, Qing Liu, Tiantang Fan, Fengzhen Liu","doi":"10.1177/19373384261494726","DOIUrl":"https://doi.org/10.1177/19373384261494726","url":null,"abstract":"<p><p>Vascular stents, serving as a cornerstone in the interventional treatment of cardiovascular diseases, had undergone rapid technological evolution and accumulation of clinical evidence. To systematically deconstruct the global research landscape, collaborative networks, and knowledge development pathways, we conducted a bibliometric and visual analysis of 5322 relevant articles (2015-2025) from the Web of Science Core Collection using VOSviewer and CiteSpace. The results showed that the annual publication growth had entered a plateau phase characterized by deepening integration, with the paradigm transitioning from discrete technological iteration toward system integration. Besides, keyword clustering identified four major research pillars including precision decision-making in revascularization, material innovation, personalized antiplatelet therapy, and evaluation based on clinical evidence. Notably, the intersection of materials science and biology (e.g., biodegradable polymers, immunomodulatory coatings) was the key driver of cutting-edge advancements. We further pointed out that future breakthroughs would depend on the deep integration of biodegradable smart materials, artificial intelligence-empowered precision intervention, and dynamic management based on real-world data. Therefore, we believe that the constructed knowledge graph and the proposed paradigm transition framework clarify the developmental trajectory and provide an evidence-based roadmap for future innovation.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"19373384261494726"},"PeriodicalIF":2.0,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Letter:</i> Regenerative Medicine as a New Research Culture: From Translation to Responsible Clinical Access.","authors":"Andreia Antunes, Alexandre Antunes","doi":"10.1177/19373384261494728","DOIUrl":"https://doi.org/10.1177/19373384261494728","url":null,"abstract":"","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"19373384261494728"},"PeriodicalIF":2.0,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing Primary Human Myogenic Spheroids and Single Cells for Skeletal Muscle Tissue Engineering.","authors":"Rebecca Wüst, Mendy Minne, Lieven Thorrez, Heidi Declercq","doi":"10.1177/19373384261494748","DOIUrl":"https://doi.org/10.1177/19373384261494748","url":null,"abstract":"<p><p>Skeletal muscle tissue engineering aims to create bioartificial muscle (BAM) constructs that recapitulate key structural and functional features of native skeletal muscle for applications including regenerative medicine, disease modeling, and drug screening. While most BAM models are generated using myoblasts, multicellular building blocks such as myogenic spheroids may better mimic native cell-cell interactions and microarchitectural organization. In this study, we compared the use of primary human myogenic spheroids with single cells for skeletal muscle tissue engineering. Myogenic spheroids composed of primary human skeletal myoblasts were generated in a high-throughput manner using nonadhesive micropatterned agarose chips. Single cell myoblasts and myogenic spheroids were subsequently embedded in fibrin hydrogels and cast into silicone molds between two metal pins serving as anchor points. Results showed that maturation stage of the spheroids prior to encapsulation significantly influenced BAM morphology after 7 days. Early maturation stage-spheroids promoted enhanced cellular outgrowth, myoblast fusion, and increased myotube diameter compared with more mature spheroids, while the spheroid size did not significantly affect construct formation. Direct comparison between spheroid-based and single-cell BAMs revealed distinct phenotypic differences. Spheroid-based constructs generated fewer but larger multinucleated myotubes with higher fusion indices, whereas single-cell constructs produced a greater number of smaller myotubes. Despite these differences in morphology, no significant changes were observed in the expression of myosin heavy chain isoforms, indicating comparable myogenic differentiation under the tested conditions. Together, these findings demonstrate that primary human myogenic spheroids represent an effective alternative building block for skeletal muscle tissue engineering and provide insight into how cell assembly strategies influence muscle tissue formation.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"19373384261494748"},"PeriodicalIF":2.0,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of CRISPR dCas9 Lentiviral Transduction in Nucleus Pulposus Cells for Use in Tissue Engineering.","authors":"Evan Davison Kotler, Sajjad Ashraf, J Paul Santerre, Rita A Kandel","doi":"10.1177/19373384261477635","DOIUrl":"10.1177/19373384261477635","url":null,"abstract":"<p><p>Efficient delivery of large gene-editing plasmids, such as the mCherry-CRISPR dCas9 system, into nucleus pulposus (NP) cells is a key step in generating sufficient cells for tissue-engineered intervertebral disc (IVD) constructs and other regenerative therapies for degenerative disc disease (DDD). However, the transfection and transduction of these environmentally sensitive cells remain challenging. This study aimed to identify the best protocol for delivery with minimal cytotoxicity and greatest efficiency. Transfection conditions in HEK293T cells were evaluated using Lipofectamine 3000, Lipofectamine Classic, and ViaFect at different reagent-to-DNA ratios and DNA amounts. Transfection efficiency was quantified by flow cytometry based on mCherry expression. Lentivirus was produced and concentrated by comparing PEG8000, a commercial Lenti-X concentrator, and ultracentrifugation. For NP cell transduction, polybrene and protamine sulfate were tested at multiple concentrations to maximize efficiency and viability. The optimized protocol was validated by delivering a CRISPR/dCas9 Synergistic Activation Mediator (SAM) system to activate endogenous FOXA2, and by seeding the resulting cells onto membranes to assess <i>in vitro</i> NP-like tissue formation. Lipofectamine 3000 at a 2:1 reagent-to-DNA ratio with 0.5 µg DNA per well yielded the highest transfection efficiency in HEK293T cells while minimizing cytotoxicity. Coprecipitation methods for lentiviral concentration, particularly the in-house PEG8000 concentrator, were better than ultracentrifugation. Protamine sulfate at 30 µg/mL yielded efficient NP cell transduction with higher viability than polybrene, as assessed by survival after antibiotic selection. Application of this protocol upregulated endogenous FOXA2 mRNA and protein expression, demonstrating functional efficacy. SAM-FOXA2 cells produced thicker tissue on membrane inserts than SAM controls, confirming compatibility of the protocol with downstream tissue engineering applications. Therefore, an optimized protocol balancing high transfection/transduction efficiency with minimized cytotoxicity was developed, supporting tissue-engineered IVD constructs and other CRISPR-based regenerative therapies for DDD.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"310-320"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Alignment of Structural Proteins in Tissue Constructs Measured by Polarized Raman Spectroscopy.","authors":"Maedeh Lotfi, Janny Piñeiro Llanes, Ghatu Subhash, Chelsey S Simmons, Malisa Sarntinoranont","doi":"10.1177/19373384261483930","DOIUrl":"10.1177/19373384261483930","url":null,"abstract":"<p><p>Structural protein alignment plays a vital role in determining the mechanical function and biological performance of engineered tissues. During tissue remodeling, alignment of fibrous components such as collagen and actin can evolve with cellular forces and geometric constraints. Previously, we developed a polarized Raman spectroscopy (PRS) technique that provides a nondestructive, label-free method to quantify protein alignment in tissues and hydrated constructs. Spectral data are analyzed using principal component analysis (PCA) to compute an amplitude alignment metric (AAM), reflecting molecular orientation. In this study, we employed PRS to quantify the spatiotemporal evolution of protein alignment in myoblast-seeded collagen constructs over a 5-day incubation period. Raman spectra were collected at seven polarization angles (0°-180°) across three defined regions-middle center (MC), middle edge (ME), and corner edge (CE)-on days 1, 2, and 5. PRS suggested a region-dependent temporal redistribution of alignment patterns, with early higher alignment at MC and later increased alignment at CE. On day 1, MC exhibited the highest alignment (AAM = 0.71 ± 0.38), while CE showed minimal organization (0.16 ± 0.23). By day 5, alignment was highest at CE (0.62 ± 0.28), while MC decreased (0.57 ± 0.36), indicating a spatial redistribution of matrix organization. There were significant main effects of region and region-by-time interaction, highlighting location- and time-dependent realignment. Within the CE region, alignment on days 2 and 5 was significantly higher than on day 1, consistent with delayed but sustained peripheral alignment. Qualitative immunofluorescence imaging provided complementary visualization of increasing fiber density and directional organization over time. Together, these findings show that PRS, with PCA-based spectral analysis, can detect early spatial and temporal changes in molecular anisotropy within myoblast-seeded collagen constructs. The observed region-dependent alignment changes are consistent with the combined influence of cellular contractility, boundary constraints, and matrix remodeling. Future studies with matched acellular controls and broader remodeling stages and construct conditions will help clarify matrix-associated contributions to PRS-derived alignment during engineered tissue maturation.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"333-341"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring a Novel Whole-Ovary Decellularization Technique for Developing Suitable Ovarian Bioscaffolds.","authors":"Jiaxin Jiang, Yangxin Zhang, Anita Touch, Suxiu Sun, You Yang, Xiaohong Li, Zhun Xiao","doi":"10.1177/19373384261484012","DOIUrl":"10.1177/19373384261484012","url":null,"abstract":"<p><p>Artificial ovary construction requires a biocompatible scaffold that mimics native extracellular matrix. This study developed a novel decellularization technique combining physical preconditioning (freeze-thaw cycle, multipoint needling, and 10% NaCl hypertonic solution) with shortened chemical detergent exposure (0.5% sodium dodecyl sulfate, 1% Triton X-100, and 2% sodium deoxycholate) for intact porcine ovaries. Compared to conventional chemical-only decellularization, the novel method better preserved glycosaminoglycan and collagen content, induced smaller changes in tissue elastic modulus, and supported higher granulosa cell fusion density <i>in vitro</i>. After subcutaneous implantation in mice, the novel scaffolds promoted greater cell infiltration and neovascularization, while triggering a lower M1-macrophage response. These results demonstrate that integrating physical steps enhances decellularization efficiency and biocompatibility, offering a promising strategy for generating functional ovarian bioscaffolds.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"321-332"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148875921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Letter:</i> Safety Testing of Tissue Regenerative Devices.","authors":"John A Jansen","doi":"10.1177/19373384261477632","DOIUrl":"10.1177/19373384261477632","url":null,"abstract":"","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"295-296"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148707756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularization of Cartilage Xenograft with Supercritical CO₂.","authors":"George S Corpuz, Xue Dong, Sophia Salingaros, Carlos Urrea de la Puerta, Nicholas A Vernice, Gillian M O'Connell, Hector F Salazar, Jini Jeon, David M Bednarski, Eric Eisenhut, Tony Eisenhut, Lawrence J Bonassar, Jason A Spector","doi":"10.1177/19373384261477631","DOIUrl":"10.1177/19373384261477631","url":null,"abstract":"<p><p>Decellularized cartilage xenograft is an emerging alternative to autograft or allograft techniques, though its application has been limited by the toxicity that conventional chemical decellularizing agents have on tissue structure and the environment. We describe a nontoxic, scalable, and environmentally sustainable ovine cartilage decellularization protocol utilizing supercritical CO₂(scCO₂). Five scCO₂ decellularization protocols were evaluated in various ovine cartilage dimensions, of which the multicycle scCO₂ method was selected for <i>in vivo</i> evaluation of ovine zested cartilage, ovine minced cartilage, and human minced cartilage. Cartilage groups were enclosed in porous polylactic acid (PLA) scaffolds and implanted subcutaneously on rat dorsa along with an empty scaffold control group. Rats were euthanized after 1, 3, and 6 months for volumetric, histological, and biomechanical analysis of implants. The efficiencies of scCO₂ decellularization was enhanced by multicycle process or additional reagents, as well as the smaller dimensions of processed cartilage, as confirmed by DNA quantification and nucleic staining. Preserved structural integrity was evidenced by intact cartilage structure, lacunae components, and collagenous content postprocessing on histology. Implantation in a murine model for 6 months demonstrated excellent neo-tissue ingrowth with minimal inflammatory response. Biomechanical testing revealed no significant differences in moduli or hydraulic permeability of minced and zested samples, before and after the decellularization treatment. The resulting stiffness after implantation mimicked the physical properties of other cartilaginous tissues. In conclusion, we demonstrate the effective decellularization of ovine cartilage graft with a scCO₂ protocol while maintaining native tissue architecture and biomechanical properties with a minimal immunogenic response <i>in vivo</i>. Further development of this approach promises to augment surgeon choice of materials used for cartilage reconstruction.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"297-309"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148723764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Method for Fabricating Long Decellularized Scaffolds from Skeletal Muscle.","authors":"Maira Z Cordelle, Giulia Biasi, Sarah J B Snelling, Pierre-Alexis Mouthuy","doi":"10.1177/19373384261463762","DOIUrl":"10.1177/19373384261463762","url":null,"abstract":"<p><p>Skeletal muscle tissue engineering (SMTE) is a rapidly evolving field with applications spanning regenerative medicine, disease modeling, drug screening, and biohybrid robotics. Effective SMTE requires scaffolds that reproduce the anisotropic architecture and mechanical properties of native muscle while supporting macroscale tissue formation. Decellularized tissues are strong candidates; however, existing approaches face key limitations. Whole-muscle decellularization requires complex perfusion systems and often fails to fully clear the tissue core. In contrast, minced-tissue processing completely disrupts native architecture and necessitates technically demanding reconstruction. As a result, producing long, continuous scaffolds needed to model or restore physiologically relevant muscle units remains challenging. Here, we present an intermediate strategy that enables the fabrication of long, aligned scaffolds from native muscle bundles. Bundles exceeding 10 cm in length were dissected to preserve native alignment and subjected to mild detergent-based decellularization, achieving efficient removal of cellular material while maintaining extracellular matrix structure and mechanics. The resulting scaffolds supported myogenic cell adhesion, proliferation, and differentiation, demonstrating their suitability for <i>in vitro</i> muscle tissue culture. This accessible approach provides a straightforward route to generate macroscale, structurally faithful skeletal muscle scaffolds, bridging the gap between whole-muscle and minced-tissue decellularization methods.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"277-290"},"PeriodicalIF":2.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148369859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}