Tissue engineering. Part C, Methods最新文献

{"title":"Development of a Complex Human <i>In Vitro</i> Model of Endochondral Ossification.","authors":"Encheng Ji, Amaia Garmendia Urdalleta, Janneke Witte-Bouma, Gert-Jan Kremers, Nunzia Di Maggio, Andrea Banfi, Eric Farrell, Andrea Lolli","doi":"10.1177/19373341251378152","DOIUrl":"https://doi.org/10.1177/19373341251378152","url":null,"abstract":"<p><p>During development and regeneration, bone is formed by endochondral ossification (EO) through the remodeling of a cartilage template. This complex process involves multiple cell types and interactions that cannot currently be modeled <i>in vitro</i>. This study aimed to develop a novel tissue-engineered human <i>in vitro</i> model of certain aspects of the early stages of EO by integrating cartilage which undergoes mineralization, self-assembled vascular networks, and osteoclasts into a single system. We first studied the dynamics of osteoclastogenesis and vascularization in an <i>in vivo</i> model of stromal cell-mediated EO, to inform our <i>in vitro</i> system. Next, we aimed to develop a fully human cell-based three-dimensional model of EO by combining pediatric bone marrow stromal cells differentiating into chondrocytes, osteoclasts derived from human CD14+ monocytes, and human umbilical vein endothelial cells and adipose-derived stromal cells as vessel-forming cells. We investigated how mineralizing cartilage affects osteoclast and vessel formation <i>in vitro</i> through separate cartilage-osteoclasts and cartilage-vessels cocultures. Finally, we combined these elements and established a complex <i>in vitro</i> model that supports the functionality of all these cell types and recapitulates chondrogenesis, cartilage mineralization, vessel formation and osteoclastogenesis. This integrated approach reaches unprecedented complexity and will enable new tissue engineering strategies to model skeletal diseases or cancer metastasis to the bone.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocomposite Hydrogels for Wound Management: A Bibliometric Review of Research Trends and Developments.","authors":"Shuilan Bao, Ting Ma, Yali Yang, Jia Zhang, Yiren Wang, Li Yao, Ping Zhou, Yun Zhou, Yunfei Li","doi":"10.1177/19373341251372883","DOIUrl":"10.1177/19373341251372883","url":null,"abstract":"<p><p>The unique advantages and broad applicability of nanocomposite hydrogels in wound care have become an indispensable driving force for innovative therapeutic strategies. However, comprehensive reviews of their latest research progress, application trends, and strategies remain insufficient. To address this, the present study employs bibliometric methods to systematically analyze the literature on nanocomposite hydrogels in wound care, covering various dimensions, including publication years, major contributing countries and institutions, core authors, publication distribution, and keyword co-occurrence networks. The analysis reveals a significant upward trend in academic attention to this field, with a steady increase in publications. Subsequently, we delve into four research hotspots, including the intelligent responsiveness of nanocomposite hydrogels, their adjustable drug release performance, their ability to promote cell proliferation and differentiation, and their innovative integration with stem cell therapy. Then, we explore the application features of nanocomposite hydrogels in wound healing, focusing on their roles in anti-inflammatory and infection control, promoting cell proliferation and angiogenesis, and providing moisturization and mechanical support. Finally, we discuss the challenges and emerging development trends in wound care using nanocomposite hydrogels, including deep integration with sensor technology, advancements toward artificial intelligence and multifunctionality, and optimization of biosafety. This study provides valuable insights and new perspectives for the future development of nanocomposite hydrogels in wound care.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 9","pages":"309-333"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Resolution Cone-Beam Tomography for Assessing Angiogenesis in Jawbone Regeneration Models.","authors":"Sibylle Vital, Gaël Sylvain, Benjamin Salmon, Claire Bardet, Catherine Chaussain, Mostafa EzEldeen, Reinhilde Jacobs, Francesca Mangione","doi":"10.1177/19373341251378381","DOIUrl":"10.1177/19373341251378381","url":null,"abstract":"<p><p>Orofacial bone tissue engineering addresses bone loss caused by trauma, malformations, or tumors, enabling restoration and implant rehabilitation. Angiogenesis plays a crucial role in osteogenesis by ensuring nutrient and oxygen transport essential for bone regeneration. Preclinical large animal models are vital for translational research and require noninvasive, nondestructive methods aligned with 3Rs principles (Replacement, Reduction, and Refinement) to assess angiogenesis. This study proposes high-resolution cone-beam computed tomography subtraction angiography (HR-CBCT-SA) adapted for the orofacial region as an innovative method for monitoring angiogenesis during jawbone regeneration. Three Yucatan minipigs with a surgically created buccal wall jawbone defect per hemimandible were followed for 90 days by CBCT-SA to assess vascular remodeling. Morphometric parameters, including vessel number, node count, radius, and length, were analyzed and validated against histological morphometry. CBCT-SA revealed vascular dynamics during healing. By day 10, increased vessel and node counts along with reduced vessel radius and length indicated neoangiogenesis. At day 30, vessel maturation was aligned with transition of fibrous tissue to osteoid matrix deposition. By day 90, vascular metrics stabilized, reflecting bone remodeling phases characterized by replacement of lamellar and medullary bone replacement. Extrabony vascular networks underwent more pronounced changes than intrabony vessels, underscoring the leading role of periosteum in regeneration. Histology validated CBCT-SA findings, although resolution limitations prevented detection of vessels smaller than 500 µm. Nevertheless, CBCT-SA captured angiogenic changes over time and supported nondestructive monitoring without compromising tissue integrity. This study establishes HR-CBCT-SA as a reliable, nondestructive imaging technique for assessing vascular changes during jawbone regeneration in preclinical models. It demonstrates significant translational potential because of the clinically validated use of CBCT-angiography. Advances in artificial intelligence (AI)-driven image analysis are expected to enhance sensitivity and accuracy, improving vascular assessment. Moreover, this approach can be extended for investigating vascular-related oral pathologies (e.g., radiochemical osteonecrosis of the jaws), offering valuable tool to advance research in jawbone regeneration.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 9","pages":"334-341"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on Polyurethane Dipping Double-Layer Membrane Coating Method for Developing Polymer-Covered Stents.","authors":"So Yeun Choi, Jeong Chan Lee, Il Won Suh, Chan Hee Park, Cheol Sang Kim","doi":"10.1177/19373341251379757","DOIUrl":"https://doi.org/10.1177/19373341251379757","url":null,"abstract":"<p><p>A stent is a medical device that is inserted into a narrowed or blocked area to normalize the flow when blood or body fluids do not flow smoothly. Covered stents coated with various materials such as silicone and expanded polytetrafluoroethylene (e-PTFE) are mainly used. However, these materials have various disadvantages, such as difficulty adding drugs or applying them to blood vessels. Electrospinning technology offers advantages in multifunctionality, including drug release and biodegradability. However, when coating a stent with an electrospun membrane, there are unresolved problems such as delamination of the membrane during stent surgery due to the weak nature and contraction of the electrospun fiber. Therefore, we studied fabricating by combining the dipping method and the electrospinning method a covered stent composed of a double-layer membrane using polyurethane (PU). It was confirmed that the double-layer membrane developed in this study has high mechanical properties, excellent adhesion to the stent, and can significantly improve the mechanical properties of the stent. This method is expected to overcome the limitations of existing cover stents manufactured using the electrospinning method by increasing the adhesive strength between the stent wire and the membrane.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 9","pages":"342-349"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: The Role of Artificial Intelligence in Biomaterials Development: A Paradigm Shift.","authors":"John A Jansen","doi":"10.1177/19373384251369935","DOIUrl":"10.1177/19373384251369935","url":null,"abstract":"","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"281-282"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144837736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric Analysis of Nanomaterials for Spinal Cord Injury Repair.","authors":"Yali Yang, Jia Zhang, Shuilan Bao, Yiren Wang, Zhongjian Wen, Shouying Chen, Li Yao, Yanhua Chen, Ping Zhou, Yun Zhou","doi":"10.1177/19373341251368846","DOIUrl":"https://doi.org/10.1177/19373341251368846","url":null,"abstract":"<p><p>This bibliometric analysis, conducted on 735 publications from the Web of Science Core Collection database up to April 16, 2025, sheds light on the evolving landscape of nanomaterials in spinal cord injury (SCI) repair. Utilizing tools such as Bibliometrix, VOSviewer, and CiteSpace, the study reveals a significant and exponential growth in literature within this field since 2020, marked by an impressive average annual increase of 13.16%. China has emerged as the global leader in research output, contributing 347 articles, with the United States closely following. Prominent institutions such as Jinzhou Medical University and Zhejiang University have played pivotal roles in advancing this domain. The research has predominantly centered around critical areas including nanoparticles, drug delivery systems, strategies for neural regeneration, and the modulation of inflammation. A notable shift in research focus has been observed in recent years, with keyword trends evolving from foundational cellular investigations toward more applied aspects such as regenerative medicine, the construction of supportive scaffolds, and crucial steps toward clinical translation. This highlights the inherent multidisciplinary potential of nanomaterials in addressing the complex challenges of SCI repair. Despite China's dominant publication volume, the analysis underscores a critical need to deepen fundamental research and foster stronger international collaborations. Looking ahead, future research endeavors should strategically prioritize the development of intelligent nanocarriers, cultivate robust interdisciplinary translational research initiatives, and establish standardized preclinical validation protocols. These targeted efforts are essential to accelerate the crucial transition of promising laboratory findings into effective clinical applications for patients suffering from SCIs.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 8","pages":"283-299"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the Effects of Cyclical Masticatory Forces in a 3D Oral Mucosal Model <i>in Vitro</i>.","authors":"Samantha Robins, Vehid Salih, Alastair Lomax, Sian Crow, Zoe Brookes, Andrew Foey, Simon A Whawell","doi":"10.1177/19373341251368861","DOIUrl":"10.1177/19373341251368861","url":null,"abstract":"<p><p>This study describes the development of a three-dimensional (3D) oral mucosal model (OMM) to investigate how oral tissues respond to masticatory forces. The OMMs replicated key features of human oral mucosa, such as stratified keratinocyte telomerase-immortalized gingival keratinocytes (TIGK) layers and fibroblast-populated collagen matrices. Cyclical mechanical forces (0-10 N) for 2 h applied to the model caused force-dependent changes in the histological structure, including thinning of the epithelium and collagen matrix and cell displacement at higher forces. Lactate dehydrogenase (LDH) cytotoxicity assays revealed that 10 N forces led to significant cell damage (about 50% cell death) in TIGK monolayers, whereas lower forces (1-5 N) caused minimal damage. OMMs showed reduced cell death (∼15% at 10 N), indicating better resilience presumably due to their 3D architecture. Additionally, force-dependent increases in the release of the proinflammatory cytokines IL-6 and IL-8 were observed, with lower responses in OMMs compared with monolayer cultures. This study demonstrates that OMMs can be used to model the effects of masticatory forces on the response of the oral mucosa in denture wearers and has been utilized to investigate the effects of a denture adhesive on the inflammatory response of the OMM to pressure.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"300-308"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Cytometry by Time-of-Flight Staining for Peripheral Blood and Bone Marrow Samples.","authors":"Yosuke Susuki, Issei Shinohara, Masatoshi Murayama, Mayu Morita, Chao Ma, Alexa K Pius, Qi Gao, Simon Kwoon-Ho Chow, Stuart B Goodman","doi":"10.1177/19373341251360986","DOIUrl":"https://doi.org/10.1177/19373341251360986","url":null,"abstract":"<p><p>Cytometry by time-of-flight (CyTOF) enables comprehensive immune profiling for translational research. However, challenges such as signal variability, nonspecific binding, and antibody incompatibility can compromise data quality. This study presents an optimized CyTOF staining protocol for human peripheral blood mononuclear cells and bone marrow aspiration concentrate samples, addressing these challenges by refining antibody conjugation with polymer X8, saponin use, and fixation protocols. Preliminary data indicate improved staining for key markers (CD14, CD16, and CD19), enhancing signal consistency and clarity. These findings advance the utility of CyTOF in orthopaedic research and immune profiling for diseases such as osteonecrosis of the femoral head.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 7","pages":"261-270"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine Placental Cotyledon-Derived Hydrogel: Methodology to Produce a Substrate for Tissue Engineering.","authors":"Raí André Querino Candelária, Igor S Cordeiro, Maria Angélica Miglino, Rodrigo S N Barreto","doi":"10.1177/19373341251360957","DOIUrl":"10.1177/19373341251360957","url":null,"abstract":"<p><p>Bioengineering aims to develop biomaterials that closely mimic the native extracellular matrix (ECM) to support tissue regeneration. This study presents a detailed protocol for producing hydrogels derived from decellularized bovine placental cotyledons. Bovine placentas at 4-5 months of gestation (<i>n</i> = 10) were subjected to vascular perfusion with increasing concentrations of sodium dodecyl sulfate (0.01-1%) and Triton X-100 (1%), which effectively removed cellular components. Decellularization efficacy was confirmed by histological (hematoxylin and eosin and 4',6-diamidino-2-phenylindole [DAPI] staining), molecular, and structural analyses, including residual genomic DNA quantification averaging 9.1 ng/mg of dry tissue. The ECM scaffolds were enzymatically digested using 0.1% (w/v) pepsin in 0.01 M HCl and reconstituted with sodium alginate at concentrations of 5%, 8%, 10%, and 12% (w/v). Crosslinking was achieved with 1% calcium chloride. Among the tested formulations, hydrogels containing 12% alginate demonstrated greater mechanical stability and preserved three-dimensional architecture, including interconnected porosity, as evidenced by scanning electron microscopy. Cytocompatibility was evaluated by culturing canine adipose-derived mesenchymal stem cells on both decellularized biomaterials and hydrogels. DAPI staining revealed nuclei after 7 and 25 days of culture, indicating cell presence and distribution throughout the constructs. These results indicate that bovine cotyledon-derived ECM hydrogels maintain structural and biochemical features favorable for cell interaction and may serve as adaptable platforms for tissue engineering, dermal repair, and three-dimensional cell culture.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"271-279"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T Cells Enhance Tissue Complexity and Function to Study Fibrosis in 3D Skin-Like Tissue Models.","authors":"Isha Singh, Madeline Morrisson, Sasha Shenk, Helen Jarnagin, Johanna Hauer, Ayesha Lobo, Lev Brown, Tamara Houck, Liora Altman-Sagan, Patricia A Pioli, Michael L Whitfield, Jonathan A Garlick","doi":"10.1177/19373341251360742","DOIUrl":"https://doi.org/10.1177/19373341251360742","url":null,"abstract":"<p><p>Fibrosis causes altered tissue structure and function in multiple organs due to a complex interplay between inflammatory cells, myofibroblasts, and extracellular matrix (ECM) components. While it is known that T cells play a role in tissue fibrosis, it remains unclear how they modulate cellular interactions to activate fibrogenesis. Since conventional monolayer cell cultures do not mimic the tissue complexity and cellular heterogeneity in the fibrotic tissue environment, there is a need to bridge the gap between monolayer cultures and <i>in vivo</i> animal studies of fibrosis by providing a more predictive 3D model for preclinical drug screening and mechanistic studies of fibrotic diseases. We have developed 3D skin-like tissues harboring blood-derived human T cells that offer a model to better understand the role these cells play in the pathogenesis of tissue fibrosis. In the current study, we constructed skin-like tissues harboring T cells, fibroblasts, macrophages, and keratinocytes and analyzed them using tissue analysis and single-cell RNA sequencing (scRNA-seq). Skin-like tissues constructed with fully autologous cells (donor-matched fibroblasts and T cells) or nonautologous cells (mismatched fibroblasts and T cells) derived from patients with scleroderma (SSc) demonstrated normal distribution of tissue markers of epithelial differentiation and proliferation. T cells in these tissues were viable and functional as seen by elevated IL-6 production by enzyme-linked immunosorbent assay, expression of alpha smooth muscle actin in fibroblasts, and scRNA-seq. We used scRNA-seq to identify five distinct T cell subpopulations: CD8 T cells (identified by KLRK1 and CD8A), proliferating CD4 T cells (identified by PCNA, MKI67, and CD4), activated CD4 T cells (identified by IL2RA, RORA, and CD4), naïve CD4 T cells (identified by CCR7 and CD4), and Th17 CD4 T cells (identified by KLRB1, RORA, IL2RA, and CD4). Fabrication of complex 3D tissues are an important step toward establishing tissue engineering approaches to study fibrosis in multiple diseases, including SSc, idiopathic pulmonary fibrosis, as well as liver and kidney fibrosis. Understanding the roles of T cells in the ECM environment and their interactions with fibroblasts will support the development of novel treatments to reverse fibrosis and restore normal tissue and organ function.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 7","pages":"248-260"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}