Tissue engineering. Part C, Methods最新文献

{"title":"Optimizing Decellularized Human Umbilical Vein as a Scaffold for Vascular Tissue Engineering.","authors":"Danang Himawan Limanto, Thomas Jatiman, Ni Kadek Sulistyaningsih, Abu Rizal Sudwikatmono Johan, Rozi Aditya Aryananda, Asra Al Fauzi, Pratiwi Soesilawati, Heri Suroto","doi":"10.1177/19373384261417176","DOIUrl":"10.1177/19373384261417176","url":null,"abstract":"<p><strong>Background: </strong>The demand for effective vascular grafts continues to increase with the increasing prevalence of cardiovascular disease. The decellularized human umbilical vein (HUV) offers a promising scaffold for cellular removal, extracellular matrix (ECM) preservation, and balanced biocompatibility.</p><p><strong>Objective: </strong>To evaluate the efficacy of a sodium dodecyl sulfate (SDS)-based decellularization protocol for HUV preparation intended for future stem cell seeding.</p><p><strong>Methods: </strong>HUVs were obtained from four full-term cesarean deliveries (37-39 weeks; total length 210 cm) and assigned to a control group or five SDS-treated groups (0.5% SDS for 6, 12, and 24 h; 1% SDS for 12 and 24 h). Following decellularization, scaffolds were freeze-dried, gamma-sterilized, and analyzed for DNA content, cell viability (MTT assay), collagen/elastin retention, and histological and ultrastructural changes.</p><p><strong>Results: </strong>The 0.5% SDS for 6 h group achieved optimal results, with significant cell clearance (1.82 ± 1.75 vs. 5.55 ± 2.49 cells/field; <i>p</i> = 0.009) and DNA reduction (59.6 ± 22.62 vs. 258.09 ± 107.63 ng/µL), while maintaining cell viability (87.05 ± 17.14% vs. 84.79 ± 14.3%; <i>p</i> = 0.781). Collagen (20.80 ± 5.26% vs. 27.34 ± 5.34%; <i>p</i> = 0.152) and elastin (21.13 ± 5.02% vs. 24.47 ± 8.01%; <i>p</i> = 0.437) retention were comparable to controls.</p><p><strong>Conclusions: </strong>A 0.5% SDS 6 h decellularization protocol effectively removed cellular components while preserving ECM integrity and biocompatibility, offering a balanced and feasible approach for preparing HUV scaffolds for vascular tissue engineering applications.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"13-23"},"PeriodicalIF":2.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Printing Colloidal Gels: Navigating the Printability Barrier.","authors":"Marissa R Perez, Vasiliki K Kolliopoulos, Sadhana A Chari, Udayabhanu Jammalamadaka, Antonios G Mikos","doi":"10.1177/19373384251406774","DOIUrl":"10.1177/19373384251406774","url":null,"abstract":"<p><p>Bioprinting continues to expand as a preferred fabrication technology as new biomaterials emerge. These biomaterials must be biocompatible, in addition to exhibiting suitable printing characteristics. Printability is usually gauged by metrics such as extrudability, fiber morphology, and shape fidelity, and optimized via design of experiments or machine learning methods. However, these optimization techniques often overlook dynamic variables such as time-dependent behavior and batch-to-batch variability that can significantly affect the quality of printed structures. In this study, we 3D-printed colloidal hydrogels to investigate the effects of printing parameters and elapsed time on overall hydrogel print quality.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 12","pages":"391-395"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory Biomaterials for Implant Osseointegration: Knowledge Mapping (2005-2025).","authors":"Yue Li, Geng Liu, Chunxia Chen","doi":"10.1177/19373384251406748","DOIUrl":"10.1177/19373384251406748","url":null,"abstract":"<p><p>This study used bibliometric analysis to comprehensively map the research landscape of immunomodulatory biomaterials enhancing implant osseointegration between 2005 and 2025. Publications retrieved from the Web of Science Core Collection were analyzed to identify temporal trends, contributing countries, institutions, journals, cocitation networks, and keyword evolution. A total of 419 articles were identified. The annual output and citations increased steadily, with China, the United States, and Australia as major contributors. The terms '3D printing', 'scaffolds', and 'macrophage polarization' emerged as recent hotspots, reflecting a shift from mechanistic exploration to clinical translation. Compared with prior reviews focusing mainly on mechanisms, this study also analyzed the depth of international collaboration, clinical orientation of journals, and bottlenecks in translational application. The field has evolved from theoretical construction (osteoimmunomodulation theory) to technological innovation (nanoengineering, dynamic response design). Future work should integrate intelligent responsive biomaterials with multiomics validation to accelerate the transition from passive repair to active regulation in bone regeneration.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 12","pages":"415-431"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evolution and Frontiers of Piezoelectric Materials for Bone and Cartilage Regeneration Research.","authors":"Jingying Liu, Yijing Han, Cheng Hu, Yunbing Wang, Chengqi He","doi":"10.1177/19373384251405475","DOIUrl":"10.1177/19373384251405475","url":null,"abstract":"<p><p>Bone and cartilage injuries are highly prevalent and arise from diverse pathogenic mechanisms, placing a substantial burden on patients' health, quality of life, and on families and society. Piezoelectric materials, inspired by tissue engineering concepts and the intrinsic piezoelectricity of human tissues, can harness the physiological electrical microenvironment to enhance tissue regeneration. To better understand the development of this field, we employed data from the Web of Science Core Citation (WoSCC) database as the core and primary focus for conducting bibliometric research and applied tools including Bibliometrix, Origin, Python, CiteSpace, and VOSviewer. A total of 388 publications from 46 countries were identified, with China, the United States, and Iran being the leading contributors. Fangwei Qi had the highest publication output, while C. Ribeiro had the highest cocitation frequency. The most productive institutions were Shanghai Jiao Tong University, the Fourth Military Medical University, and the University of Chinese Academy of Sciences. <i>ACS Applied Materials & Interfaces</i> published the largest number of articles. The most frequent keywords included \"bone regeneration,\" \"osteogenic differentiation,\" \"piezoelectric,\" \"scaffolds,\" and \"hydroxyapatite.\" Furthermore, we employed Scopus as a validation database to cross-verify the publication trends and keyword hotspots derived from WoSCC, with the results demonstrating a high degree of consistency. These findings reveal that research on the role of piezoelectric materials in bone and cartilage regeneration is expanding rapidly, highlighting the current hotspots and emerging trends and providing valuable insights to guide future studies in this area.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 12","pages":"396-414"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium Phosphate Cements: From the 1980s to the 2020s.","authors":"Andreza S Andrada, Daniel A Maria, Sidney N da Silva, Luis A L Dos Santos, John A Jansen","doi":"10.1177/19373384251406766","DOIUrl":"10.1177/19373384251406766","url":null,"abstract":"<p><p>Over the past four decades, calcium phosphate cements (CPCs) have emerged as promising materials for bone repair due to their biocompatibility, osteoconductivity, and <i>in situ</i> hardening properties. Developed from a combination of calcium phosphate (CaP)-based powder and a liquid phase, CPCs undergo a chemical reaction when mixed, forming a crystalline solid structure at body temperature. This hardening process is characterized by its mildly exothermic reaction, offering significant advantages compared with cements like polymethylmethacrylate, commonly used in orthopedic surgeries. Over more than four decades of research, various modifications have been introduced to the physical, mechanical, and biological properties of CPCs, making them adaptable to a wide range of clinical applications, from craniofacial surgery to bone tissue engineering and drug delivery systems. Despite all the advancements, the widespread clinical use of CPCs still faces significant challenges, such as limitations in mechanical strength, degradation rate, and osteoinductive properties. This article provides a comprehensive historical and technical overview of CPC development from their initial discovery in 1980s to current innovations in formulation, physicochemical, mechanical, and biological properties. It highlights crucial milestones in each decade, covering the evolution of these cements and presenting the challenges that remain until today.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 12","pages":"432-461"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Anti-Inflammatory Effect of Bupivacaine-Meloxicam Therapy in a Rabbit Model of Arthrofibrosis.","authors":"Kareme D Alder, Mason F Carstens, Cole E Bothun, Oliver B Dilger, Ashley N Payne, Roman Thaler, Mark E Morrey, Joaquin Sanchez-Sotelo, Daniel J Berry, Amel Dudakovic, Matthew P Abdel","doi":"10.1177/19373384251397946","DOIUrl":"10.1177/19373384251397946","url":null,"abstract":"<p><p>Arthrofibrosis is a common complication following total knee arthroplasty and is the result of a dysregulated immuno-inflammatory cascade, which culminates in excessive scar deposition by myofibroblasts within the knee joint. Pharmacotherapies for arthrofibrosis are limited, with treatment reliant on manipulation under anesthesia or lysis of adhesions with or without component revision. Bupivacaine (a local anesthetic) and meloxicam (a nonsteroidal anti-inflammatory drug) combination therapy may exert antifibrotic properties by limiting inflammatory cell recruitment and the subsequent release of profibrotic cytokines. The purpose of this study was to evaluate the effects of this combination therapy in a rabbit model of arthrofibrosis by evaluating live and postsacrifice knee biomechanics and gene expression of posterior knee capsule tissue. Forty New Zealand white rabbits were equally divided into two experimental groups and prospectively studied to assess knee passive extension angles (PEA), terminal posterior capsular stiffness, and the inflammatory milieu of the posterior knee joint capsule. Experimental limbs with and without combination therapy showed similar live PEAs and terminal posterior capsular stiffness, with no significant differences between the groups. Gene expression analysis, however, revealed significant reduction in inflammatory genes but not profibrotic genes. In summary, bupivacaine and meloxicam combination therapy did not exert antifibrotic effects in a rabbit model of arthrofibrosis but significantly reduced the expression of inflammatory markers in the local environment of the posterior capsule.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 11","pages":"384-390"},"PeriodicalIF":2.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13094741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145514056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Calcium Phosphate Ceramics: The Living Bridge Between Biology and Biomaterials.","authors":"John A Jansen","doi":"10.1177/19373384251397945","DOIUrl":"https://doi.org/10.1177/19373384251397945","url":null,"abstract":"","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 11","pages":"381-383"},"PeriodicalIF":2.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145514139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterial-Derived Multimodal Cell-Regulatory Signals for Tissue Regeneration.","authors":"Xiumei Wang","doi":"10.1177/19373384251390661","DOIUrl":"10.1177/19373384251390661","url":null,"abstract":"<p><p>In tissue engineering and regenerative medicine, biomaterials have transitioned from passive structural supports to dynamic platforms capable of actively modulating regenerative microenvironments. Their tunable physical and chemical properties, combined with the capacity for controlled release of bioactive signals or extrinsic stimuli, allow precise regulation of stem, immune, and tissue-specific cell behaviors. However, single-modality of signal remains insufficient to recapitulate the multifactorial and spatiotemporally coordinated processes underlying complex tissue regeneration. From a biomaterial perspective, we proposed biomaterial-based multimodal tissue engineering strategy, focusing on the synergy of multimodal cell-regulatory signals for enhanced tissue regeneration. These multifunctional biomaterials serve as advanced artificial regenerative niches, capable of delivering coordinated multimodal signals to precisely guide cellular behavior and tissue formation. Inspired by bionic design principles, decoding the compositional, structural, mechanical, and biological parameters of the native extracellular matrix-and elucidating their regulatory effects and molecular mechanisms on cellular activities-has informed the development of multifunctional biomaterials for tissue regeneration. Key material properties-spanning mechanical, topological, biochemical, and dynamic characteristics-can be strategically engineered to function as distinct yet complementary regulatory signals in this multimodal approach.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 10","pages":"351-354"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145368847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Three-Dimensional Preclinical Model for Investigating Cartilage Regeneration, Incorporating Physiological and Pathological Mechanical Loading.","authors":"Mingjing Zhu, Jianfeng Jin, Hadi Seddiqi, Astrid D Bakker","doi":"10.1177/19373384251390654","DOIUrl":"10.1177/19373384251390654","url":null,"abstract":"<p><p>The development and implementation of novel treatments for temporomandibular joint disorders (TMDs) are limited by the lack of preclinical models that closely mimic <i>in vivo</i> conditions. Specifically, models that support three-dimensional (3D) cell culture and mechanical stimulation are needed, as both factors significantly influence cell behavior and, consequently, the success of TMDs treatments. We designed a novel 3D <i>in vitro</i> model using cells subjected to dynamic moderate loading (simulating chewing) or constant excessive loading (simulating clenching) applicable as an <i>in vivo</i>-mimetic system for TMDs research. In this study, cylindrical constructs (diameter: 6 mm, height: 3 mm), composed of 3% agarose containing mouse bone marrow mesenchymal stem cells (BMSCs), were cultured in chondrogenic medium. Cells in these constructs exhibited a 3.0-fold increase in <i>Sox9</i> RNA expression compared to cells inside agarose constructs cultured in proliferation medium and a 2.5-fold increase compared to micromass cultures (golden standard). After confirming the chondrogenic potential, we subjected the cell-agarose constructs to dynamic and constant mechanical loading using a custom-designed bioreactor. The constructs were divided into five groups: unloaded controls, dynamic loading (5% compression at 1 Hz), and constant loading at 10%, 20%, and 30% compression for 20 min per day. Finite element modeling analysis revealed that by increasing the strain compression level, the uniformity of displacement and von Mises stress distribution within the hydrogel will be reduced. Moreover, 30% constant loading compromised the structural integrity of the agarose constructs. Nitric oxide production by BMSCs was significantly elevated in response to 5% dynamic loading (2.8-fold), 20% constant loading (2.7-fold), and 30% constant loading (2.4-fold) after 20 min of stimulation compared to controls. Furthermore, 5% dynamic loading significantly upregulated <i>c-Fos</i> (2.0-fold) and <i>c-Jun</i> (1.5-fold) gene expression relative to controls, while 10% and 20% constant loading also significantly increased <i>c-Jun</i> expression (1.5-fold and 1.6-fold, respectively). Interestingly, 20% constant strain reduced the number of live cells compared with 10% constant loading. These findings demonstrate that a novel 3D preclinical model allowing to investigate cartilage regeneration under <i>in vivo</i>-like physiological and pathological mechanical stimuli has been established, providing a promising platform for future studies on chondroinductive agents and mechanical loading treatments for the TMDs.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":"31 10","pages":"367-379"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145368859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of Developed Mononuclear Cell Separator for Regenerative Therapy Is Comparable to Manual.","authors":"Orie Saino, Takahiro Shioyama, Kenichi Nomura, Yuko Ogawa, Hodaka Makino, Soichiro Adachi, Yukino Shinozaki, Hirotsugu Kubo, Tsubasa S Matsui, Kenji Kusumoto, Kenichi Morita, Akihiko Taguchi","doi":"10.1177/19373384251389349","DOIUrl":"10.1177/19373384251389349","url":null,"abstract":"<p><p>Regenerative therapy involving transplanted bone marrow mononuclear cells (BM-MNCs) and hematopoietic stem cells (HSCs) is markedly effective against many diseases. However, manual MNC separation requires skilled labor and cell-processing centers. Thus, efforts have been targeted toward fractionating MNCs using cell separators. A double-blind, placebo-controlled study of myocardial infarction using BM-MNCs separated by an existing device was conducted, and no therapeutic effects were found. The development of a cell separator to replace manual techniques would significantly contribute to the widespread application of BM-MNC therapy. Therefore, we developed a BM-MNC separation device that can reproduce manual separation. We changed the shape of the injection port on the centrifuge container and determined its circuit to improve the performance of HSC separation and remove degenerative red blood cells (RBCs). We assessed HSC recovery and degenerative RBC removal rates using fluorescence-activated cell sorting. Additionally, we evaluated the therapeutic effects of cells separated using our device in mouse models of stroke. The HSC recovery and degenerative RBC removal rates using the device were comparable to those obtained using manual separation. We also confirmed the therapeutic effects of BM-MNCs separated by the device in the models. The new automated device could replace manual cell separation and render cell-based therapy using BM-MNC feasible without laborious manual tasks at dedicated cell-processing centers.</p>","PeriodicalId":23154,"journal":{"name":"Tissue engineering. Part C, Methods","volume":" ","pages":"355-366"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}