Tissue Engineering. Part B, Reviews最新文献

{"title":"Research Progress in Tissue Engineering of Temporomandibular Joint Condylar Cartilage.","authors":"Jinjin Ma, Yan Feng, Xinxin Ni, Qing Yang, Jun Lin","doi":"10.1089/ten.teb.2025.0073","DOIUrl":"10.1089/ten.teb.2025.0073","url":null,"abstract":"<p><p>The temporomandibular joint (TMJ) comprises the mandibular condyle, the articular surface of the temporal bone, and the articular disc. The articular cartilage in the TMJ is classified as fibrocartilage, which has distinct zones: the fibrous, proliferative, mature, and hypertrophic zones. TMJ osteoarthritis (TMJOA) is a prevalent condition affecting the TMJ, with its pathogenesis involving multiple factors such as trauma, occlusal instability, joint overload, and others. Current treatment options encompass noninvasive, minimally invasive, and surgical interventions. However, no definitive cure has been found. Tissue engineering offers a novel approach to treating TMJOA by promoting cartilage repair and regeneration by constructing artificial cartilage grafts made from a combination of cells, bioactive factors (BFs), and biodegradable scaffolds. Among the scaffolds commonly used in research are hydrogels, nanoparticles, and three-dimensional-printed structures, with mesenchymal stem cells serving as the primary cell source. Additionally, exosomes and gene therapy have shown promise in TMJOA treatment. Despite significant progress, optimizing the integration of seed cells, BFs, and scaffold materials remains a critical focus for future research. This article provides an in-depth review of the latest advancements in TMJ condylar cartilage tissue engineering.Impact StatementThis review comprehensively overviews tissue engineering advancements for temporomandibular joint condylar cartilage regeneration. It highlights key progress in scaffolds, cell-based therapies, bioactive factors, and gene therapies. The review offers valuable insights for future research and potential clinical applications, contributing significantly to developing novel therapeutic strategies for temporomandibular joint osteoarthritis.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"296-312"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric Analysis and Trends of Organoid Technology in Tissue Engineering and Disease Modeling.","authors":"Die Hu, Lei Sun, Xuekun Xing","doi":"10.1177/19373368261469465","DOIUrl":"https://doi.org/10.1177/19373368261469465","url":null,"abstract":"<p><p>Organoid technology, as an innovative three-dimensional (3D) cell culture method, has undergone rapid advancement in the fields of tissue engineering and disease model construction in recent years. Using data retrieved from the PubMed database, this study employed bibliometric methods to systematically analyze 774 relevant publications from 2006 to 2026, focusing on research output, national and institutional distribution, collaboration networks, journal and author influence, and keyword evolution. The results show a significant increase in organoid research output, with China and the United States being the main contributing countries, and active international collaboration. Leading institutions, such as Harvard Medical School, occupy prominent positions in this field, with research covering multidisciplinary directions including cell culture, tissue scaffolds, microfluidic chips, and 3D bioprinting. Keyword analysis revealed core hotspots such as cell differentiation, tissue engineering methods, and multipotent stem cells, with 3D printing and induced pluripotent stem cells emerging as new research frontiers. The study indicates that organoid technology demonstrates substantial utility in simulating human tissue microenvironments, advancing precision medicine, and drug screening. Despite challenges related to vascularization, production standardization, and cost constraints, the development of high-throughput screening platforms and the enhancement of international collaboration systems will promote its clinical translation and sustainable development.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261469465"},"PeriodicalIF":4.3,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Bioprinting-Enabled Organoids for Engineering the Tumor Microenvironment.","authors":"Yehua Li, Jiale Shi, Qin Zhang, Hai Huang, Shen'ao Zhou","doi":"10.1177/19373368261469463","DOIUrl":"https://doi.org/10.1177/19373368261469463","url":null,"abstract":"<p><p>The tumor microenvironment (TME) is a complex and dynamic system composed of immune cells, stromal cells, and extracellular matrix (ECM), whose composition and spatial architecture critically influence tumor initiation, progression, invasion, metastasis, and response to immunotherapy. However, traditional research models such as two-dimensional cell coculture, patient-derived xenograft models, and conventional patient-derived organoid (PDO) systems remain limited in simulating the complexity and physiological relevance of the TME. Three-dimensional (3D) bioprinting technology, with its precise spatial control capabilities and advantages in multicellular integration, has emerged as a promising engineering tool for constructing TME-like models with controlled spatial architecture, tunable matrix properties, and programmable multicellular organization. This review examines the principles and capabilities of major 3D bioprinting technologies (extrusion-based, droplet-based, photopolymerization-based) and evaluates their applications in reconstructing key TME features such as matrix remodeling, vascularization, immune cell integration, and drug response modeling with organoids. Importantly, we distinguish between bioprinted organoid-based systems and bioprinted tumor cell models, highlighting their differences in biological fidelity and translational relevance. In addition, challenges including limited integration of PDO, constraints in multicellular coculture, and insufficient long-term functional stability continue to restrict the physiological relevance of these models. Overall, this review provides an engineering-oriented perspective on the current state of bioprinted TME models and outlines practical directions for developing more physiologically relevant platforms for cancer research, drug screening, and precision oncology.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261469463"},"PeriodicalIF":4.3,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional Integrated Hydrogels for Tissue Regeneration and Repair.","authors":"Ying Liu","doi":"10.1177/19373368261469767","DOIUrl":"https://doi.org/10.1177/19373368261469767","url":null,"abstract":"<p><p>Multifunctional integrated hydrogels have emerged as advanced biomaterials that integrate diverse functional properties, including bioactivity, mechanical adaptability, controlled drug release, and electrical/magnetic responsiveness, exhibiting substantial potential in tissue regeneration and repair. These hydrogels provide a versatile platform to mimic the complex microenvironment of native tissues, thereby facilitating cell proliferation, differentiation, and tissue remodeling. Despite rapid progress in their design and development, optimizing performance for specific tissues (e.g., bone, cartilage, skin, nerve, myocardium) remains challenging. Current research focuses on innovative design strategies and the incorporation of key functional modules to tailor hydrogels for targeted regenerative applications. Moreover, translating these materials from bench to bedside requires overcoming barriers related to biocompatibility, scalability, regulatory approval, and long-term functional stability. This review comprehensively summarizes recent advances in multifunctional integrated hydrogels, highlights their applications across different tissue regeneration scenarios, and critically evaluates translational challenges. By synthesizing cutting-edge findings, this work aims to guide the development of next-generation smart tissue engineering scaffolds and accelerate their clinical adoption, ultimately advancing regenerative medicine.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261469767"},"PeriodicalIF":4.3,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproducibility and Validation Challenges in AI-Driven Scaffold Design for Bone Regeneration.","authors":"Mohammadamin Damsaz, Farnaz Heidari Laybidi, Zohreh Moradipour, Hossein Sobhani, Niloufar Shavali, Mohammad Mahdi Rezaei Nayeh, Elham Gholami, Amirmohammad Dolatabadi, Amir Raee, Mohammad Hosseini Hooshiar","doi":"10.1177/19373368261467436","DOIUrl":"https://doi.org/10.1177/19373368261467436","url":null,"abstract":"<p><p>Artificial intelligence (AI) is increasingly applied to bioink formulation and bioprinting process control in tissue engineering (TE). Yet, translational progress remains constrained by small datasets, limited cross-platform validation, and weak links between computational predictions and biological outcomes. This review critically evaluates experimentally validated AI applications across scaffold-based bone regeneration, spanning materials design, fabrication control, and biological assessment. Physics-informed models are often more robust and generalizable than purely data-driven approaches, whereas transfer learning remains challenging due to variability in cellular responses and fabrication conditions. To address gaps in existing reporting standards, to address gaps in current reporting recommendations, we propose the AI-TE reporting framework, incorporating TE-specific requirements for biological validation, fabrication documentation, and translational readiness. Impact StatementDespite the rapid growth of AI-driven scaffold design for bone regeneration, reproducibility, and validation remain insufficiently addressed across current studies. This review identifies key methodological gaps and synthesizes practical validation and reporting priorities to improve reliability and translational relevance.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261467436"},"PeriodicalIF":4.3,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148424345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanobiological Design of Bioinspired Polymeric Scaffolds for Bone Regeneration.","authors":"Shengyu Lyu, Jing Wang, Xiaoyan Ren, Xiaojun Li, Peihui Ding, Justine C Lee, Qi Zhou","doi":"10.1177/19373368261460324","DOIUrl":"https://doi.org/10.1177/19373368261460324","url":null,"abstract":"<p><p>Bone regeneration presents a significant clinical challenge due to the complex interplay between biological processes and the local mechanical environment. While polymeric scaffolds are widely utilized for their tunable physicochemical properties, traditional designs often fail to replicate the dynamic mechanical cues required for optimal tissue remodeling. This review critically examines the mechanobiological design of bioinspired polymeric scaffolds. We first categorize native bone mechanics and the role of mechanical stimuli-such as stiffness, fluid shear, and stability-in regulating the fracture healing cascade. We then bridge these biological principles with advanced fabrication strategies, analyzing how natural, synthetic, and composite polymers can be engineered to mimic the hierarchical stiffness and bioactivity of native bone. Furthermore, we discuss the role of computational modeling (e.g., Finite Element Analysis) in predicting scaffold performance and highlight emerging technologies, including 4D printing and piezoelectric scaffolds, which offer time-dependent and mechano-electrical adaptability. Finally, we address current barriers to clinical translation and propose future directions for mechanically adaptive systems that actively guide regeneration.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261460324"},"PeriodicalIF":4.3,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation Targets Mitochondrial Homeostasis for Diabetic Wound Healing.","authors":"Feng Lai, Yuxiu Mai, Zhaodan Wang, Chao Wang, Shulan Xu","doi":"10.1177/19373368261460337","DOIUrl":"https://doi.org/10.1177/19373368261460337","url":null,"abstract":"<p><p>Diabetes mellitus is a global public health problem, and impaired wound healing is a complication that significantly reduces patients' quality of life. Dysregulation of mitochondrial homeostasis is a key pathological feature contributing to impaired wound healing in diabetes. This dysregulation increases oxidative stress, resulting in impaired energy metabolism, endothelial dysfunction, and prolonged inflammatory responses. Photobiomodulation (PBM) is a noninvasive therapy that has been successfully used to promote diabetic wound healing by modulating mitochondrial homeostasis via multiple mechanisms. In this review, we have systematically summarized the following roles of PBM in restoring mitochondrial homeostasis to accelerate diabetic wound healing: improving mitochondrial dysfunction and oxidative stress through cytochrome C oxidase in the electron transport chain, thereby enhancing oxidative phosphorylation and adenosine triphosphate production; modifying mitochondrial dynamics by inhibiting the expression of dynamin-related protein 1 and promoting mitofusin-2 expression to restore mitochondrial morphology and function; reducing inflammation and promoting macrophage polarization from the M1 to M2 phenotype; activating signaling pathways (e.g., VEGF, PI3K/AKT/mTOR/GSK3-β, AMPK, RAS/MAPK, JAK/STAT, NF-κB, TGF-β/Smad) to enhance cell proliferation and angiogenesis and resolve inflammation. Beyond monotherapy, this review synthesizes the burgeoning field of PBM in combination with advanced therapeutic strategies, such as hydrogels, nanomaterials, small-molecule drugs, adipose-derived stem cells, and extracellular vesicles, highlighting their synergistic potential for enhanced efficacy. Finally, this review critically addresses the prevailing challenges in clinical translation, particularly the lack of standardized treatment parameters, and proposes future research directions. This comprehensive overview aims to solidify the scientific foundation of PBM and inspire the design of integrated, precision therapeutic strategies for diabetic wound management.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261460337"},"PeriodicalIF":4.3,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148340423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotechnology-Driven Noninvasive and Targeted Therapies for Anaplastic Thyroid Carcinoma.","authors":"Li Ma, Xuejiao Peng, Yilin Li, Gaowei Yan, Yiming Wang, Weiqin Chang","doi":"10.1177/19373368261460338","DOIUrl":"https://doi.org/10.1177/19373368261460338","url":null,"abstract":"<p><p>Anaplastic thyroid carcinoma (ATC) is a highly aggressive and treatment-resistant form of thyroid cancer, characterized by poor prognosis, rapid metastasis, and resistance to conventional therapies. Current treatment options, including surgery, chemotherapy, and radiation, are often ineffective in controlling the disease, underscoring the urgent need for novel therapeutic approaches. Nanotechnology has emerged as a promising strategy to overcome these challenges, offering innovative solutions for targeted drug delivery and noninvasive therapeutic modalities. This review explores the latest advancements in nanotechnology-driven strategies for treating ATC, with a particular focus on noninvasive therapies such as sonodynamic therapy and nano-pulse stimulation, as well as the development of nanomaterial-based drug delivery systems. These systems, including RNA-based nanocarriers, radionuclide-labeled nanoparticles, and nanoclay-based delivery systems, provide enhanced specificity and efficacy, overcoming the limitations of traditional treatments. Additionally, dual-modality theranostic systems, which combine diagnostic imaging and therapeutic payloads, hold great promise in improving early detection and real-time treatment monitoring. Despite the promising preclinical results, challenges such as tumor heterogeneity, drug resistance, and biocompatibility of nanomaterials remain in the clinical use. This review provides a comprehensive overview of the potential of nanotechnology to revolutionize the treatment of ATC, offering new hope for patients with this highly aggressive malignancy.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261460338"},"PeriodicalIF":4.3,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148340470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Restoration to Regeneration: A Review of 3D Printing Strategies in Digital Dentistry.","authors":"Ziya Lao, Qianwen Deng, Ting Lin, Lvhua Guo, Jingwen Wu, Wen Ren","doi":"10.1177/19373368261460323","DOIUrl":"https://doi.org/10.1177/19373368261460323","url":null,"abstract":"<p><p>Three-dimensional (3D) printing technology, or additive manufacturing, has fundamentally transformed medical paradigms by shifting dentistry from traditional subtractive manufacturing to an era of personalized, biological regeneration. This review provides a comprehensive overview of the primary 3D printing classifications currently utilized, including stereolithography, fused deposition modeling, selective laser sintering, and material jetting, analyzing their specific technical principles and material capabilities. We systematically categorize their applications across diverse dental disciplines, ranging from endodontics, periodontal disease management, and prosthodontics to orthodontics, maxillofacial reconstruction, and temporomandibular joint regeneration. Beyond the fabrication of anatomical models and surgical guides, this article critically evaluates the efficacy of these technologies in constructing bioactive scaffolds and cell-laden hydrogels designed to induce osteogenesis and angiogenesis. Special emphasis is placed on the paradigm shift from passive mechanical restoration to active biological regeneration, highlighting the potential of bioprinting in restoring physiological vitality to native tissues. Despite current barriers regarding the trade-off between mechanical durability and biological activity, as well as vascularization challenges, the convergence of artificial intelligence and 4D printing promises to establish 3D printing as a foundational standard for the next generation of regenerative dental therapeutics.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261460323"},"PeriodicalIF":4.3,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148340489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Pathogenesis of and Regenerative Strategies for Osteonecrosis of the Femoral Head.","authors":"Zhengang Ding, Liwei Fu, Jiang Wu, Qi Wang, Guangzhao Tian, Shengbo Huang, Yazhe Zheng, Jianhao Wu, Zhichao Zhang, Zhixing Zhang, Chuanyang Long, Xiang Sui, Shuyun Liu, Quanyi Guo, Baoshan Xu, Mingxue Chen","doi":"10.1177/19373368261460246","DOIUrl":"10.1177/19373368261460246","url":null,"abstract":"<p><p>Osteonecrosis of the femoral head (ONFH) is a progressive, multifactorial bone disease characterized by ischemia-induced osteocyte death, microenvironmental imbalance, and failed tissue regeneration. According to recent advances in pathophysiological understanding, vascular injury, oxidative stress, and inflammatory storms form a pathogenic cascade leading to osteogenic dysfunction, adipogenic lineage drift of mesenchymal stem cells (MSCs), and epigenetic alterations that exacerbate bone degeneration. Despite improvements in early detection, traditional interventions-including bisphosphonates, hyperbaric oxygen therapy, and surgical decompression-have limited efficacy, particularly in the mid-to-late stages. This review systematically synthesizes emerging regenerative approaches across three domains: (1) Cellular and molecular therapies: Autologous MSC transplantation, exosomes, and apoptotic extracellular vesicles restore osteogenesis, modulate immunity, and promote angiogenesis, while gene-editing technologies such as CRISPR/Cas9 enhance MSC functionality. (2) Nanobiomaterial synergy: Enzyme-mimetic nanozymes and multifunctional polymeric scaffolds improve lesion targeting, reactive oxygen species clearance, and microenvironmental regulation. (3) Advanced bioengineering: Organoid models and 3D-bioprinted living joint prostheses enable the integration of vascularization, mechanical support, and precise drug delivery, representing transformative strategies in personalized repair. Together, these innovations highlight a future paradigm shift from passive support to active, mechanism-targeted regeneration, offering new hope for structural and functional reconstruction in ONFH. Multidisciplinary integration-bridging materials science, stem cell biology, and digital medicine-will be essential for successfully developing functional cures.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261460246"},"PeriodicalIF":4.3,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148340414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}