Tissue Engineering. Part B, Reviews最新文献

{"title":"Reconceptualizing Mesenchymal Stromal Cell Sheets: From Delivery Tool to Models of Morphogenesis.","authors":"Natalia A Alexandrushkina, Valentina S Glazieva, Maria A Vodopetova, Roman Yu Eremichev, Yu-Chen Hu, Pavel I Makarevich","doi":"10.1177/19373368261448752","DOIUrl":"https://doi.org/10.1177/19373368261448752","url":null,"abstract":"<p><p>Over the past three decades, cell sheet technology has evolved from its original scaffold-free approach developed for epithelial cells into a versatile platform applicable to mesenchymal stromal cells (MSC). Despite numerous experimental achievements and convincing preclinical outcomes, MSC-based cell sheets have yet to reach the stage of a clinically reproducible product. This gap reflects not so much the technological limitations of the method as an incomplete understanding of its biological nature. MSC-based cell sheets represent more than a vehicle for cell delivery; they are self-organizing systems governed by intrinsic biophysical and morphogenetic principles. Their structural maturation involves (1) cellular condensation, (2) extracellular matrix deposition, and (3) contractile remodeling-processes that mirror the early phases of granulation tissue formation. Viewing the cell sheet as an <i>in vitro</i> model of connective tissue regeneration opens new research avenues extending beyond its therapeutic applications. This review summarizes the key milestones in the development of MSC-derived cell sheet technology, identifies major challenges and conceptual inconsistencies, and discusses the potential of studying these constructs as autonomous biological systems. The integration of mechanobiology, spatial omics technologies, and tissue engineering approaches may help reconceptualize MSC-based cell sheets both as tools for translational therapy and as a fundamental model for studying self-organizing regenerative processes.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261448752"},"PeriodicalIF":4.6,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental Follicle Stem Cells and Biological Scaffolds in Dental Regenerative Medicine.","authors":"Lu Xuan, Xiaomei Liu, Jiahao Chen, Zhuomin Sha, Jiarui Yang, Yuanna Zheng, Hua Li, Xuepeng Chen","doi":"10.1177/19373368261419610","DOIUrl":"https://doi.org/10.1177/19373368261419610","url":null,"abstract":"<p><p>Dental follicle stem cells (DFSCs) originate from the dental follicle during tooth development and possess multilineage differentiation potential, contributing to periodontal tissue regeneration, bone repair, and immunomodulation. This review highlights the recent advances in the application of DFSCs and biological scaffolds for regenerative medicine, with a focus on oral and craniofacial tissue. DFSCs exhibit key advantages for regenerative therapies, including high accessibility, robust self-renewal capacity, and multipotent differentiation potential, enabling their differentiation into odontogenic (dentin- and enamel-forming), osteogenic, and fibroblastic lineages. We discuss the embryonic origin of DFSCS and their unique ability to maintain stable cellular properties in long-term <i>in vitro culture</i>. Importantly, DFSCs play a pivotal role in tooth morphogenesis, periodontal tissue formation, and craniofacial bone regeneration, making them promising for functional oral tissue restoration. A critical aspect of DFSC-based regeneration is the integration with bioactive scaffolds, which provide structural support, promote cell adhesion, proliferation, and differentiation, and facilitate vascularization. We analyze how scaffold properties, such as biodegradability, porosity, and permeability, influence DFSC behavior and therapeutic outcomes. Finally, we explore future challenges and opportunities in optimizing DFSC-scaffold interaction, emphasizing advancements in biomaterial design and emerging bioengineering technologies. Preliminary evidence suggests that integrating DFSCs with engineered scaffold systems may offer potential benefits for personalized regenerative therapies, though further validation is required before clinical translation. Such approaches could contribute to advancing tooth and craniofacial reconstruction strategies. This review consolidates existing insights and explores potential avenues for future research to support advancements in DFSC-based regenerative medicine.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261419610"},"PeriodicalIF":4.6,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147609828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dormancy in Metastatic Colorectal Cancer: Tissue Engineering Opportunities for <i>In Vitro</i> Modeling.","authors":"Sabrina N VandenHeuvel, Lucia L Nash, Shreya A Raghavan","doi":"10.1089/ten.teb.2025.0009","DOIUrl":"10.1089/ten.teb.2025.0009","url":null,"abstract":"<p><p>Colorectal cancer (CRC) recurs at a striking rate, specifically in patients with liver metastasis. Dormant CRC cells disseminated following initial primary tumor resection or treatment often resurface years later to form aggressive, therapy-resistant tumors that result in high patient mortality. Routine imaging-based screenings often fail to detect dormant cancer cell clusters, and there are no overt symptomatic presentations, making dormant CRC a major clinical challenge to diagnose and treat. Tissue engineering approaches are ideally suited to model dormant cancer cells and enable the discovery of therapeutic vulnerabilities or unique mechanistic dependencies of dormant CRC. Emerging evidence suggests that tissue-engineered approaches have been successfully used to model dormant breast and lung cancer. With CRC responsible for the second most cancer-related deaths worldwide and CRC patients commonly experiencing recurrence, it is essential to expand dormancy models to understand this phenomenon in the context of CRC. Most published <i>in vitro</i> models of CRC dormancy simplify the complex tumor microenvironment with two-dimensional culture systems to elucidate dormancy-driving mechanisms. Building on this foundation, future research should apply tissue engineering methods to this growing field to generate competent three-dimensional models and increase mechanistic knowledge. This review summarizes the current state of <i>in vitro</i> CRC dormancy models, highlighting the techniques utilized to give rise to dormant CRC cells: nutrient depletion, anticancer drugs, physical extracellular matrix interactions, and genetic manipulation. The metrics used to validate dormancy within each model are also consolidated to demonstrate the lack of established standards and the ambiguity around comparing studies that have been validated differently. The methods of these studies are organized in this review to increase comprehensibility and identify needs and opportunities for future bioengineered <i>in vitro</i> models to address dormancy-driven mortality in patients with CRC liver metastasis.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"126-142"},"PeriodicalIF":4.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Biomaterial Scaffolds for Eyelid Reconstruction: A Synthesis of Experimental Findings.","authors":"Jincheng Liu, Mange Zhang, Mengling Zhou, Qingyi Wang, Xin Jiang, Qin Huang","doi":"10.1089/ten.teb.2024.0364","DOIUrl":"10.1089/ten.teb.2024.0364","url":null,"abstract":"<p><p>This review synthesizes experimental findings on various biomaterial scaffolds used in eyelid reconstruction. It examines the structural properties, cellular responses, and functional outcomes of scaffolds such as chitosan, poly(propylene glycol fumarate)-2-hydroxyethyl methacrylate, poly(propylene glycol fumarate) - type I collagen (PPF-Col), decellularized matrix-polycaprolactone, branched polyethylene, collagen, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate, and poly(lactic-co-glycolic acid. These scaffolds exhibit diverse mechanical and biological properties, with some demonstrating good biocompatibility, tunable properties, and potential for tissue repair. However, there are limitations, including concerns about long-term functionality and a lack of comprehensive evaluations. This review highlights the need for multifunctional scaffolds that combine lid replacement and ocular surface function restoration, as well as the establishment of standardized research methods. The goal is to guide future innovation in the field and improve the quality of life for patients with eyelid defects.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"143-156"},"PeriodicalIF":4.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Regulation of Pericellular Matrix Synthesis During Articular Cartilage Tissue Engineering.","authors":"Marloes van Mourik, Florencia Abinzano, Keita Ito","doi":"10.1089/ten.teb.2024.0316","DOIUrl":"10.1089/ten.teb.2024.0316","url":null,"abstract":"<p><p>Articular cartilage, vital to the health and functioning of joints, remains challenging to regenerate. The pericellular matrix (PCM) is critical for transducing biophysical stimuli to the articular chondrocytes (ACs) that it envelops. Given the mechanobiological sensitivity of ACs, it is pivotal in maintaining the chondrogenic phenotype and the production of extracellular matrix (ECM) during articular cartilage tissue engineering. While the maintenance of the native PCM significantly improves the quality of neocartilage, current isolation methods are limited. A solution to this challenge is facilitating ACs to regenerate their PCM. However, the regulation of PCM synthesis remains poorly understood, hindering the development of effective tissue engineering strategies. This narrative review aims to provide a comprehensive analysis of the complex interplay between extracellular cues and intracellular pathways regulating PCM synthesis during articular cartilage tissue engineering. Our analysis reveals that mechanical cues, such as material stiffness and mechanical stimulation, are the primary regulators of PCM synthesis. Additionally, the use of scaffold-free techniques potentially affects the structuring of newly created PCM. Tuning these stimuli can significantly impact the quality of the formed PCM, ultimately influencing neocartilage quality. Furthermore, we highlight intracellular mechanisms involved in the transduction of these extracellular cues, including actin polymerization, yes-associated protein and transcriptional coactivator with PDZ-binding motif localization, and transforming growth factor beta-induced Smad signaling. Although the current literature suggests the involvement of these signaling pathways in regulating the synthesis of PCM components, we found that studies investigating these processes in ACs are lacking. Elucidating the relationships between extracellular stimuli, intracellular signaling, and the expression of PCM components could provide a framework for designing new cartilage tissue engineering approaches that facilitate proper PCM synthesis. Ultimately, this can improve ECM quality and advance articular cartilage regeneration.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"157-170"},"PeriodicalIF":4.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Multicellular Neural Tissue Using Three-Dimensional Printing Technology: Cell Interaction.","authors":"Zhixiang Li, Tong Su, Yujie Yang, Huan Zhao","doi":"10.1089/ten.teb.2024.0323","DOIUrl":"10.1089/ten.teb.2024.0323","url":null,"abstract":"<p><p>The study of the human nervous system remains challenging due to its inherent complexity and difficulty in obtaining original samples. Three-dimensional (3D) bioprinting is a rapidly evolving technology in the field of tissue engineering that has made significant contributions to several disciplines, including neuroscience. In order to more accurately reflect the intricate multicellular milieu of the <i>in vivo</i> environment, an increasing number of studies have commenced experimentation with the coprinting of diverse cell types. This article provides an overview of technical details and the application of 3D bioprinting with multiple cell types in the field of neuroscience, focusing on the challenges of coprinting and the research conducted based on multicellular printing. This review discusses cell interactions in coprinting systems, stem cell applications, the construction of brain-like organoids, the establishment of disease models, and the potential for integrating 3D bioprinting with other 3D culture techniques.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"109-125"},"PeriodicalIF":4.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review on Pulmonary Toxicity of Silver, Zinc Oxide, Copper Oxide, and Alumina Nanoparticles.","authors":"Anjali Gupta, Divya Bajpai Tripathy, Nidhi Puri","doi":"10.1177/19373368261425114","DOIUrl":"https://doi.org/10.1177/19373368261425114","url":null,"abstract":"<p><p>Advancement in nanotechnology has rapidly led to the widespread applications of varied nanoparticles (NPs) in day-to-day life. Among them, metal and metal oxide-based NPs are significant due to their unique physicochemical properties and diversified applications. However, these properties that make them valuable can also pose unexpected, noxious threats to various organs in the human body. That is why a comprehensive consideration of NP toxicity is important for exploring their safer and effective use in varied biomedical applications. This review aims to compile current knowledge about the pulmonary toxicity of silver, zinc oxide, copper oxide, and aluminum oxide-based NPs, with a focus on the physicochemical properties affecting their pulmonary toxicity and its mechanisms. However, these studies employ high doses that are somewhat less relevant to human inhalation exposures, but with an understanding of these aspects, we can better navigate the challenges and concerns posed by metal-based NPs and work toward safer biomedical applications.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261425114"},"PeriodicalIF":4.6,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147487337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Thyroid Gland Regeneration: The Integrated Approach of Cell Biology and Bioengineering.","authors":"Tomasz Górnicki, Maria Grazia Soriano, Giuseppe Raffaele, Gabriele Maria Fortunato, Walis Jones, Antonella Cecchettini, Patrycja Szymczyk-Ziółkowska, Krzysztof Kaliszewski, Piotr Dzięgiel, Federico Vozzi, Carmelo Di Maria, Bartosz Kempisty, Giovanni Vozzi","doi":"10.1177/19373368261431206","DOIUrl":"https://doi.org/10.1177/19373368261431206","url":null,"abstract":"<p><p>The thyroid gland is an endocrine organ responsible for production of triiodothyronine and thyroxine, essential hormones that regulate human metabolism. A wide range of conditions can impair its function, leading to potential life-threatening consequences such as myxedema coma. The standard treatment for hypothyroidism is lifelong levothyroxine supplementation, which, despite being a significant therapeutic breakthrough, has notable limitations and does not fully restore quality of life for many patients. Biomimetic thyroid gland has emerged as a promising alternative treatment strategy for patients with hypothyroidism. Most research to date has focused on generating thyroid organoids from primary thyroid cells or stem cells. However, there is growing interest in other approaches, including the use of biomaterials, bioreactors, and 3D bioprinting as potential alternatives or supplementary technologies to the organoids. While <i>in vitro</i> and preclinical studies have shown encouraging results, clinical application of biomimetic thyroid gland requires further studies in several key areas, including long-term functional validation, studies on large animal models, immunological compatibility and scaffold biodegradation, and absence of standardized good manufacturing practice (GMP)-compliant production protocols.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261431206"},"PeriodicalIF":4.6,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147487376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breast Tissue Reconstruction Strategies: A Tissue Engineering Approach.","authors":"Alina Stoian, Çağdaş Duru, Faraz Chogan, Felor Biniazan, Brian Temple, Golnaz Karoubi, Siba Haykal","doi":"10.1177/19373341251372961","DOIUrl":"10.1177/19373341251372961","url":null,"abstract":"<p><p>Breast cancer remains the most commonly diagnosed malignancy among women worldwide. Standard treatment often involves mastectomy, followed by chemotherapy and/or radiation. Approximately 40% of patients undergo breast reconstruction to address the physical and psychological effects of tissue loss. Since the first autologous breast reconstruction described in 1887, both autologous and alloplastic techniques have evolved significantly to improve patient outcomes. However, current approaches are limited by issues such as the inability to restore biological breast function, suboptimal tissue integration, and concerns over long-term implant viability. Tissue engineering has emerged as a promising field capable of overcoming these limitations. Since the 1990s, advances in biomaterials, stem cell research, and regenerative strategies have enabled the development of vascularized, patient-specific constructs with potential applications in both structural and functional breast reconstruction. This review provides a comprehensive overview of the evolution of breast reconstruction techniques and the integration of tissue engineering into the field. Particular emphasis is placed on tissue engineering's role in enhancing breast cancer treatment and diagnosis while also exploring future directions toward functional restoration, including lactation.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373341251372961"},"PeriodicalIF":4.6,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogels for Pelvic Organ Prolapse: Animal Models, Hydrogel Properties, and Biomedical Applications.","authors":"Hongru Li, Mingbo Jiang, Zongyu Liu, Duoduo Fang, Limei Fan","doi":"10.1177/19373341251375073","DOIUrl":"10.1177/19373341251375073","url":null,"abstract":"<p><p>Pelvic organ prolapse (POP) is a common yet complex condition affecting women, characterized by the descent of pelvic organs due to weakened pelvic floor structures. While several treatment strategies exist, their efficacy is often limited, and complications such as surgical failure or recurrence can hinder long-term success. Hydrogels, due to their unique properties such as high-water content, biocompatibility, and flexibility, offer promising potential in the management of POP. This review summarizes various animal models of POP including abdominal wall weakness model, sustained pressure method (vaginal ball stretching), ovariectomy (OVX) model, and gene knockout model. This review further provides a comprehensive overview of the role of hydrogels in POP, highlighting their applications in tissue engineering, drug delivery, and as coatings or injectable materials for prolapsed organs. Furthermore, the challenges in their development were discussed, including material selection, degradability, mechanical properties, and long-term biocompatibility. The strategies to optimize hydrogel performance to better meet clinical needs, with an emphasis on personalization and multifunctionality, were outlined. In conclusion, while hydrogels offer significant promise, further research into their design, application methods, and clinical outcomes is crucial to fully realize their potential in the treatment of POP.Impact StatementThis review highlights the transformative potential of hydrogels in treating pelvic organ prolapse, a condition with limited long-term therapeutic success. By systematically analyzing animal models and exploring hydrogel applications in tissue repair and drug delivery, it identifies critical challenges and future directions. The insights offered lay the groundwork for personalized, multifunctional hydrogel systems, guiding future research and accelerating clinical translation.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373341251375073"},"PeriodicalIF":4.6,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}