Tissue Engineering. Part B, Reviews最新文献

{"title":"Bioactive Glass in Dentistry: A Bibliometric Analysis of Trends, Impact, and Future Directions.","authors":"Tayana Takeshova, Madina Kurmanalina, Aruzhan Aitmukhanbetova, Nadiar M Mussin, Nader Tanideh, Amin Tamadon","doi":"10.1089/ten.teb.2025.0016","DOIUrl":"https://doi.org/10.1089/ten.teb.2025.0016","url":null,"abstract":"<p><p>This bibliometric analysis examined research on bioactive glass in dentistry from 2015 to 2024, identifying key trends, its impact on dental applications, and future research directions. Data were collected from Web of Science and Scopus in April 2025, focusing on studies published between 2015 and 2024 using the keywords \"Dentistry\" AND \"Bioactive Glass.\" A total of 2114 studies from 706 sources were analyzed, involving 7471 authors with an average of 5.78 coauthors per article. The analysis used Web of Science and Scopus, which provide comprehensive access to peer-reviewed literature in dentistry and materials science. Prominent journals included <i>Dental Materials</i>, <i>Ceramics International</i>, <i>Materials</i>, <i>Journal of the Mechanical Behavior of Biomedical Materials</i>, and <i>Journal of Dentistry</i>. There was a notable increase in publications, with 52 articles in 2024. The average number of citations per document was 15.61, and the average document age was 4.72 years. Collaborative research, especially among Saudi Arabia, Egypt, China, the United States, and Brazil, was a significant trend. Leading institutions included the Egyptian Knowledge Bank, University of London, and King Abdulaziz University, reflecting substantial contributions from the Middle East, Europe, and Asia. Core research topics focused on bioactive glass, mechanical properties, nanoparticles, bioactivity, and hydroxyapatite. The study highlights a growing global interest in bioactive glass, particularly in relation to dentin hypersensitivity, remineralization, and tissue regeneration. The continued rise in publication volume and expansion of international collaborations underscore the vitality of this field. Emerging directions such as bone regeneration, antibacterial applications, and advancements in the mechanical performance of bioactive materials are likely to shape the trajectory of future research. Impact Statement This bibliometric analysis highlights the growing significance of bioactive glass in dentistry, particularly in the context of remineralization, tissue regeneration, and antimicrobial protection. The increasing volume of research, highlighted by a surge in publications and international collaborations, reflects the expanding interest in bioactive glass. Prominent research areas include remineralization, hydroxyapatite applications, and mechanical properties of bioactive materials, with implications for bone regeneration and innovative dental treatments. By identifying trends and leading contributors, this study provides a foundation for future research aimed at enhancing the clinical applications and material science of bioactive glass in dentistry.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding Intervertebral Disc Cell Populations: Challenges in Isolation and Phenotype Definition.","authors":"Joana R Ferreira, Maria Leonor Moura, Sofia Pilão, Ana Luisa Castro, Morena Fiordalisi, Catarina Leite Pereira, Joana Caldeira, Raquel M Gonçalves","doi":"10.1089/ten.teb.2024.0350","DOIUrl":"https://doi.org/10.1089/ten.teb.2024.0350","url":null,"abstract":"<p><p>Degenerative disc disease (DDD) is the main cause of back pain, one of the most impactful musculoskeletal disorders. In the last decades, significant advances have emerged in the field of intervertebral disc (IVD) biology. Despite cumulative knowledge on cell heterogeneity, there is a lack of understanding about the molecular signature of different IVD cell populations and their potential as therapeutic targets. This is not only due to progress in single-cell analysis being recent, but also to difficulties and lack of standardization in the isolation of distinct IVD cell populations based on the phenotypic markers used. Herein, we review the literature on IVD resident cells and the main challenges faced in the source, isolation, and culture of IVD cell subsets that are yet to be overcome. The profiling of IVD subpopulations and their cellular dynamics with development, aging, and disease progression will certainly advance the knowledge on IVD homeostasis and associated diseases, while contributing to the development of game-changing, personalized targeted therapies.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Regulation of Pericellular Matrix Synthesis During Articular Cartilage Tissue Engineering.","authors":"Marloes van Mourik, Florencia Abinzano, Keita Ito","doi":"10.1089/ten.teb.2024.0316","DOIUrl":"https://doi.org/10.1089/ten.teb.2024.0316","url":null,"abstract":"<p><p>Articular cartilage, vital to the health and functioning of joints, remains challenging to regenerate. The pericellular matrix (PCM) is critical for transducing biophysical stimuli to the articular chondrocytes (ACs) that it envelops. Given the mechanobiological sensitivity of ACs, it is pivotal in maintaining the chondrogenic phenotype and the production of extracellular matrix (ECM) during articular cartilage tissue engineering. While the maintenance of the native PCM significantly improves the quality of neocartilage, current isolation methods are limited. A solution to this challenge is facilitating ACs to regenerate their PCM. However, the regulation of PCM synthesis remains poorly understood, hindering the development of effective tissue engineering strategies. This narrative review aims to provide a comprehensive analysis of the complex interplay between extracellular cues and intracellular pathways regulating PCM synthesis during articular cartilage tissue engineering. Our analysis reveals that mechanical cues, such as material stiffness and mechanical stimulation, are the primary regulators of PCM synthesis. Additionally, the use of scaffold-free techniques potentially affects the structuring of newly created PCM. Tuning these stimuli can significantly impact the quality of the formed PCM, ultimately influencing neocartilage quality. Furthermore, we highlight intracellular mechanisms involved in the transduction of these extracellular cues, including actin polymerization, yes-associated protein and transcriptional coactivator with PDZ-binding motif localization, and transforming growth factor beta-induced Smad signaling. Although the current literature suggests the involvement of these signaling pathways in regulating the synthesis of PCM components, we found that studies investigating these processes in ACs are lacking. Elucidating the relationships between extracellular stimuli, intracellular signaling, and the expression of PCM components could provide a framework for designing new cartilage tissue engineering approaches that facilitate proper PCM synthesis. Ultimately, this can improve ECM quality and advance articular cartilage regeneration.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tendon-Bone Healing: Synergistic Role of Platelets and Mesenchymal Stem Cells in Tissue Engineering.","authors":"Yajie Wu, Xu Wang, Daohong Zhao, Ruke Lin, Xinfu Zhang, Xibei Lin","doi":"10.1089/ten.teb.2024.0333","DOIUrl":"https://doi.org/10.1089/ten.teb.2024.0333","url":null,"abstract":"<p><p>Repair of the tendon-bone interface (TBI) remains a significant clinical challenge due to its complex biomechanical environment and hierarchical structure. Conventional surgical approaches often fail to fully reestablish native tissue architecture and function. In recent years, tissue engineering strategies have increasingly emphasized the application of mesenchymal stem cells (MSCs) and platelet-derived products to promote regeneration. MSCs possess multilineage differentiation potential and immunomodulatory capabilities, making them attractive candidates for TBI repair. Platelets, through their rich secretome, orchestrate essential regenerative processes such as cell recruitment, angiogenesis, and immune modulation. This review explores the molecular crosstalk between MSCs and platelets, critically examines current approaches utilizing platelet-rich plasma (PRP)-MSC combinations and platelet-derived exosome therapies and underscores the urgent need for standardization to optimize therapeutic outcomes in PRP-MSC-based regenerative strategies.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Recent Applications of Stem Cell-Derived Exosomes and Hydrogels in Neurological Disorders.","authors":"Nabil Ajwad, Muzaimi Mustapha, Zamzuri Idris, Si-Yuen Lee","doi":"10.1089/ten.teb.2024.0353","DOIUrl":"10.1089/ten.teb.2024.0353","url":null,"abstract":"<p><p>Neurological disorders such as Alzheimer's disease, Parkinson's disease, and stroke pose significant challenges for conventional therapy due to the complexities of the blood-brain barrier (BBB) and the restricted delivery of drugs to the central nervous system. Exosomes, a type of small extracellular vesicle secreted by nearly all cell types, hold substantial promise as delivery vehicles for therapeutic agents in treating these conditions. Notably, stem cell-secreted exosomes have emerged as particularly effective due to their regenerative potential and natural ability to cross the BBB. Similarly, hydrogels have gained recognition as versatile biomaterials capable of supporting sustained release and targeted delivery of therapeutics. The combination of the regenerative properties of stem cell-derived exosomes (SC-Exos) with the structural and functional benefits of hydrogels offers a promising approach for enhancing neurogenesis, modulating neuroinflammation, and facilitating tissue repair. This review explores the origin, structure, and modifications of exosomes as well as the synthesis and incorporation methods of hydrogels in the therapeutic context for debilitating neurological disorders. It highlights recent advancements in using SC-Exos and hydrogels for therapeutic delivery, addressing both current challenges and future applications. Improving our understanding of hydrogels loaded with SC-Exos for cargo transportation and neural tissue regeneration may pave the way for novel therapeutic strategies.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Platelet-Rich Plasma-Based Scaffolds in Soft and Hard Tissue Regeneration.","authors":"Niloofar Khandan-Nasab, Behdad Torkamanzadeh, Behnam Abbasi, Taraneh Mohajeri, Reza Kazemi Oskuee, Amirhossein Sahebkar","doi":"10.1089/ten.teb.2024.0285","DOIUrl":"https://doi.org/10.1089/ten.teb.2024.0285","url":null,"abstract":"<p><p>Platelet-rich plasma (PRP) is a blood product with higher platelet concentrations than whole blood, offering controlled delivery of growth factors (GFs) for regenerative medicine. PRP plays pivotal roles in tissue restoration mechanisms, including angiogenesis, fibroblast proliferation, and extracellular matrix development, making it applicable across various regenerative medicine treatments. Despite promising results in different tissue injuries, challenges such as short half-life and rapid deactivation by proteases persist. To address these challenges, biomaterial-based delivery scaffolds, such as sponges or hydrogels, have been investigated. Current studies exhibit that PRP-loaded scaffolds fix these issues due to the sustained release of GFs. In this regard, given the widespread application of PRP in clinical studies, the use of PRP-loaded scaffolds has drawn significant consideration in tissue engineering (TE). Therefore, this review briefly introduces PRP as a rich origin of GFs, its classification, and preparation methods and discusses PRP applications in regenerative medicine. This study also emphasizes and reviews the latest research on the using scaffolds for PRP delivery in diverse fields of TE, including skin, bone, and cartilage repair.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electric Field-Induced Effects in Eukaryotic Cells: Current Progress and Limitations.","authors":"Daniil A Bystrov, Daria D Volegova, Sofia A Korsakova, Alla B Salmina, Stanislav O Yurchenko","doi":"10.1089/ten.teb.2025.0022","DOIUrl":"https://doi.org/10.1089/ten.teb.2025.0022","url":null,"abstract":"<p><p>Electric fields (EFs) offer a powerful tool for manipulating cells and modulating their behavior, holding significant promise for regenerative medicine and cell biology. We provide a comprehensive overview of the effects of different types of EF on eukaryotic cells with the special focus on physical mechanisms and signaling pathways involved. Direct current EF induces electrophoresis and electroosmosis, influencing cell migration, proliferation, and differentiation. Alternating current EF, through dielectric polarization and dielectrophoresis, enables cell manipulation, trapping, and sorting. Pulsed EF, particularly high-intensity, short-duration pulses, induces reversible and irreversible electroporation, facilitating drug and gene delivery. The review covers some technological aspects of EF generation, emphasizing the importance of experimental setups, and integration with microfluidic platforms for high-throughput analysis and precise manipulations. Furthermore, the synergistic potential of combining EFs with optical tweezers is highlighted, enabling fine-tuned control of cell positioning, intercellular interactions, and measurement of biophysical properties. Finally, the review addresses limitations of EF application, such as field heterogeneity and potential side effects, and outlines the directions for future studies, including developing the minimally invasive delivery methods.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioengineered Constructs as a Tissue Engineering-Based Therapy for Volumetric Muscle Loss.","authors":"Surendrasingh Y Sonaye, Prabaha Sikder","doi":"10.1089/ten.teb.2025.0017","DOIUrl":"https://doi.org/10.1089/ten.teb.2025.0017","url":null,"abstract":"<p><p>Severe skeletal muscle injuries involving substantial tissue loss can significantly impair muscle strength and functionality, reducing the quality of life for affected individuals. Such injuries, termed volumetric muscle loss, require extensive clinical intervention, as the body's innate healing mechanisms are insufficient to regenerate functional muscle. The current standard of care primarily involves autologous muscle tissue transfer, with some consideration of acellular synthetic constructs. However, both approaches have limited therapeutic efficacy, presenting challenges such as donor-site morbidity, infection risks, and suboptimal functional recovery. Over the past decade, skeletal muscle tissue engineering (SMTE) has emerged as a promising strategy for regenerating functional muscle through bioengineered constructs. Advanced biofabrication techniques, including bioprinting, have further enabled the development of synthetic constructs that closely mimic native muscle architecture. Given these advancements, a critical review of recent therapeutic strategies, their achievements, and limitations is necessary. This review examines the spectrum of bioengineered constructs developed from various biomaterials and evaluates their therapeutic potential. Special emphasis is placed on 3D bioprinting strategies and their role in creating physiologically relevant constructs for functional muscle restoration. In addition, the integration of machine learning in optimizing construct design, predicting cellular behavior, and enhancing tissue integration is discussed. The review indicates that despite significant progress in SMTE, key challenges remain, including replicating the complex structural organization of muscle tissue, minimizing fibrosis, and achieving vascularization and innervation to regenerate functional, strengthened muscle. Future research should address these barriers while prioritizing the development of translational, clinically relevant regenerative constructs. In addition, efforts should focus on advancing scalable, construct-based regenerative treatments that are readily available at the point of care and easily managed in surgical settings.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Multicellular Neural Tissue Using Three-Dimensional Printing Technology: Cell Interaction.","authors":"Zhixiang Li, Tong Su, Yujie Yang, Huan Zhao","doi":"10.1089/ten.teb.2024.0323","DOIUrl":"https://doi.org/10.1089/ten.teb.2024.0323","url":null,"abstract":"<p><p>The study of the human nervous system remains challenging due to its inherent complexity and difficulty in obtaining original samples. Three-dimensional (3D) bioprinting is a rapidly evolving technology in the field of tissue engineering that has made significant contributions to several disciplines, including neuroscience. In order to more accurately reflect the intricate multicellular milieu of the <i>in vivo</i> environment, an increasing number of studies have commenced experimentation with the coprinting of diverse cell types. This article provides an overview of technical details and the application of 3D bioprinting with multiple cell types in the field of neuroscience, focusing on the challenges of coprinting and the research conducted based on multicellular printing. This review discusses cell interactions in coprinting systems, stem cell applications, the construction of brain-like organoids, the establishment of disease models, and the potential for integrating 3D bioprinting with other 3D culture techniques.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Biomaterial Scaffolds for Eyelid Reconstruction: A Synthesis of Experimental Findings.","authors":"Jincheng Liu, Mange Zhang, Mengling Zhou, Qingyi Wang, Xin Jiang, Qin Huang","doi":"10.1089/ten.teb.2024.0364","DOIUrl":"https://doi.org/10.1089/ten.teb.2024.0364","url":null,"abstract":"<p><p>This review synthesizes experimental findings on various biomaterial scaffolds used in eyelid reconstruction. It examines the structural properties, cellular responses, and functional outcomes of scaffolds such as chitosan, poly(propylene glycol fumarate)-2-hydroxyethyl methacrylate, poly(propylene glycol fumarate) - type I collagen (PPF-Col), decellularized matrix-polycaprolactone, branched polyethylene, collagen, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate, and poly(lactic-co-glycolic acid. These scaffolds exhibit diverse mechanical and biological properties, with some demonstrating good biocompatibility, tunable properties, and potential for tissue repair. However, there are limitations, including concerns about long-term functionality and a lack of comprehensive evaluations. This review highlights the need for multifunctional scaffolds that combine lid replacement and ocular surface function restoration, as well as the establishment of standardized research methods. The goal is to guide future innovation in the field and improve the quality of life for patients with eyelid defects.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}