Tissue Engineering. Part B, Reviews最新文献

{"title":"Synergistic Effects of Therapeutic Ultrasound and Biomaterials in Osteoarthritis.","authors":"Wenjie Hou, Xiaoxia Hao, Chunran Pan, Xingru Shang, Tao Xu","doi":"10.1177/19373368251398336","DOIUrl":"10.1177/19373368251398336","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a common degenerative joint disease characterized by progressive cartilage degradation, subchondral bone remodeling, and synovial inflammation. Current treatments cannot halt or reverse OA progression, necessitating the development of novel noninvasive therapies. Therapeutic ultrasound (US), particularly low-intensity pulsed US, has demonstrated efficacy in slowing OA progression. Therapeutic US generates significant thermal and nonthermal effects through noninvasive mechanical forces, exerting biological effects and regulating cell behavior. Therapeutic US has been explored for bone and cartilage repair and shows broad potential in tissue repair when combined with biomaterials. This review summarizes the enhanced or synergistic effects of US and biomaterials in OA. This study elucidated the molecular mechanisms underlying the effects of US on synovium, cartilage, subchondral bone, and mesenchymal stem cells. Notably, the combination of US with various biomaterials can modulate cellular behavior in OA through synergistic effects, including tissue regeneration, enhanced mechanical stimulation, drug delivery, and microenvironment regulation. For each cell type, we summarize the biological mechanisms underlying the therapeutic effects of US and biomaterials, demonstrating their potential to mitigate OA progression. Furthermore, this article explores the limitations and future research prospects of combining US and biomaterials as a therapeutic strategy. Overall, the integration of US and biomaterials holds significant promise as a novel treatment for OA, with potential applications in broader musculoskeletal tissue repair and regenerative medicine.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"369-389"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances Focusing on the Application of Various Ions in Tendon-Bone Healing.","authors":"Panpan Yu, Jiamin Guo, Guiying Nie, Yinling He, Tianhong Peng, Xi Chen, Liang Li, Zhu Dai, Wei Xie","doi":"10.1177/19373368251388823","DOIUrl":"10.1177/19373368251388823","url":null,"abstract":"<p><p>The tendon-bone interface (TBI) possesses a highly intricate structure, making complete restoration of its native structure postinjury particularly challenging, which often leads to suboptimal healing outcomes. Metal ions, such as calcium (Ca²⁺), magnesium (Mg²⁺), zinc (Zn²⁺), copper (Cu²⁺), cobalt (Co²⁺), strontium (Sr²⁺), iron (Fe^2+/Fe³⁺), and lithium (Li⁺), have attached significant attention in tissue regeneration research owing to the excellent roles in promoting angiogenesis, osteogenesis, and chondrogenesis. This review systematically elucidates a comprehensive overview of the current understanding of these bioactive ions' mechanisms and their applications in TBI repair. Additionally, the review highlights the importance of incorporating metal ions into biomaterial scaffolds to enhance simultaneous multitissue regeneration while addressing current therapeutic limitations in TBI management. Finally, the review outlines future research directions for optimizing ion-based biomaterial strategies to advance TBI treatment paradigms.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"359-368"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances of Cell Printing Technology in Organoid Engineering.","authors":"Yu-Han Ho, Yuanhong Liao, Lingni Liao, Tianjiao Mao, Yimin Guan, Ren Xu","doi":"10.1089/ten.teb.2025.0048","DOIUrl":"10.1089/ten.teb.2025.0048","url":null,"abstract":"<p><p>Organoid engineering is a rapidly expanding field that involves developing miniaturized, three-dimensional (3D) structures to mimic the architecture and function of real organs. It provides a powerful platform to investigate organ development, disease modeling, and personalized medicine. Recent advances in cell printing technology, also known as bioprinting, feature high-throughput potential, precise control, and enhanced reproducibility, enabling the deposition of living cells to generate complex, 3D biological structures. Cell printing with bioinks composed of cells and supportive biomaterials has been utilized to generate <i>in vitro</i> tissues and organs with intricate architectures and functionalities to investigate normal tissue morphogenesis and disease progression. The integration of cell printing technology and organoid engineering holds tremendous potential in biomedical research. Here, we summarize recent advances in cell printing technology in developing different organoid models, creating patient-specific tissue grafts, and utilizing these models and grafts in drug testing, as well as studying disease progression. Some of these bioprinted organoids have been utilized in clinical trials, highlighting the potential of cell printing technology in future applications in tissue and organ transplantation, as well as precision medicine.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"348-358"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Regulation of Tissue Remodeling Through Chitosan-Based Hydrogels in Wound Healing Dynamics.","authors":"Reyhaneh Molaei, Atefe Hosseinkhani, Mostafa Saberian","doi":"10.1089/ten.teb.2025.0078","DOIUrl":"10.1089/ten.teb.2025.0078","url":null,"abstract":"<p><p>Effective wound healing hinges on a precisely orchestrated tissue remodeling process that restores both structural integrity and functionality. This review delineates the molecular mechanisms by which chitosan-based hydrogels revolutionize wound repair. Derived from natural chitin, chitosan uniquely combines robust antimicrobial, hemostatic, and biodegradable properties with the capacity to modulate critical intracellular signaling cascades-including transforming growth factor-β, mitogen-activated protein kinase, and PI3K/AKT. These dynamic interactions drive fibroblast proliferation, stimulate the strategic transition from type III to type I collagen deposition, and finely tune extracellular matrix reorganization, thereby mitigating excessive fibrosis and minimizing scar formation. Notwithstanding its considerable therapeutic promise, clinical translation of chitosan-based hydrogels is tempered by challenges in mechanical stability and controlled degradation. We propose that advanced material engineering-encompassing precision cross-linking, nanoparticle integration, and synergistic stem cell-based strategies-could surmount these limitations. This comprehensive synthesis of current molecular insights sets the stage for next-generation regenerative biomaterials, positioning chitosan-based hydrogels as a paradigm-shifting platform for achieving superior healing outcomes in complex clinical scenarios.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"333-347"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Bioengineering Strategies to Enhance Dental Stem Cell Therapeutic Effects in Oncology Treatments.","authors":"Ensiyeh Kordparijaei, Elaheh Ferdosi-Shahandashti, Zahra Harasani","doi":"10.1177/19373368261469473","DOIUrl":"https://doi.org/10.1177/19373368261469473","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) reside in various organs and play vital roles in maintaining tissue homeostasis and facilitating regeneration. These cells can be harvested from discarded tissues, expanded <i>in vitro</i>, and utilized as therapeutic agents for immune-related and inflammatory disorders. Their regenerative capabilities are largely mediated through interactions with immune cells. Dental stem cells (DSCs), isolated from multiple dental tissues, exhibit remarkable immunomodulatory properties and have demonstrated therapeutic efficacy in autoimmune and inflammatory diseases. However, the immunosuppressive function of MSCs/DSCs presents a unique challenge in oncology, where it can paradoxically support tumor progression. This review critically analyzes the immunoregulatory pathways involved in inflammatory and immune-related disorders, and directly links these mechanisms to the challenges within the tumor microenvironment. Furthermore, we discuss innovative bioengineering strategies to reprogram MSCs/DSCs or target their pathways for enhanced anticancer efficacy. We highlight the critical clinical limitations and the need for personalized approaches based on distinct DSC subpopulations. A deeper comprehension of this context-dependent functionality is essential for developing safe and effective MSC/DSC-based therapies.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261469473"},"PeriodicalIF":4.3,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electric Field-Induced Effects in Eukaryotic Cells: Current Progress and Limitations.","authors":"Daniil A Bystrov, Daria D Volegova, Sofia A Korsakova, Alla B Salmina, Stanislav O Yurchenko","doi":"10.1089/ten.teb.2025.0022","DOIUrl":"10.1089/ten.teb.2025.0022","url":null,"abstract":"<p><p>Electric fields (EFs) offer a powerful tool for manipulating cells and modulating their behavior, holding significant promise for regenerative medicine and cell biology. We provide a comprehensive overview of the effects of different types of EF on eukaryotic cells with the special focus on physical mechanisms and signaling pathways involved. Direct current EF induces electrophoresis and electroosmosis, influencing cell migration, proliferation, and differentiation. Alternating current EF, through dielectric polarization and dielectrophoresis, enables cell manipulation, trapping, and sorting. Pulsed EF, particularly high-intensity, short-duration pulses, induces reversible and irreversible electroporation, facilitating drug and gene delivery. The review covers some technological aspects of EF generation, emphasizing the importance of experimental setups, and integration with microfluidic platforms for high-throughput analysis and precise manipulations. Furthermore, the synergistic potential of combining EFs with optical tweezers is highlighted, enabling fine-tuned control of cell positioning, intercellular interactions, and measurement of biophysical properties. Finally, the review addresses limitations of EF application, such as field heterogeneity and potential side effects, and outlines the directions for future studies, including developing the minimally invasive delivery methods.Impact StatementThe application of electric fields (EFs) for cell manipulation and modulation of cellular functions and behavior is a promising task in regenerative medicine and cell biology. This article provides systemized and structured information about EF parameters, induced effects in cells <i>in vitro</i>, and involved signaling pathways and introduces novice biomedical engineers to research in this field.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"273-295"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Platelet-Rich Plasma-Based Scaffolds in Soft and Hard Tissue Regeneration.","authors":"Niloofar Khandan-Nasab, Behdad Torkamanzadeh, Behnam Abbasi, Taraneh Mohajeri, Reza Kazemi Oskuee, Amirhossein Sahebkar","doi":"10.1089/ten.teb.2024.0285","DOIUrl":"10.1089/ten.teb.2024.0285","url":null,"abstract":"<p><p>Platelet-rich plasma (PRP) is a blood product with higher platelet concentrations than whole blood, offering controlled delivery of growth factors (GFs) for regenerative medicine. PRP plays pivotal roles in tissue restoration mechanisms, including angiogenesis, fibroblast proliferation, and extracellular matrix development, making it applicable across various regenerative medicine treatments. Despite promising results in different tissue injuries, challenges such as short half-life and rapid deactivation by proteases persist. To address these challenges, biomaterial-based delivery scaffolds, such as sponges or hydrogels, have been investigated. Current studies exhibit that PRP-loaded scaffolds fix these issues due to the sustained release of GFs. In this regard, given the widespread application of PRP in clinical studies, the use of PRP-loaded scaffolds has drawn significant consideration in tissue engineering (TE). Therefore, this review briefly introduces PRP as a rich origin of GFs, its classification, and preparation methods and discusses PRP applications in regenerative medicine. This study also emphasizes and reviews the latest research on the using scaffolds for PRP delivery in diverse fields of TE, including skin, bone, and cartilage repair.Impact StatementPlatelet-rich plasma (PRP) is a blood product with high platelet concentrations, offering managed delivery of growth factors (GFs) for regenerative medicine. This review briefly introduces PRP as a rich source of GFs and discusses PRP-loaded scaffold applications in soft and hard tissue including skin, bone, and cartilage restoration. In the current study, the applications of PRP-loaded scaffold in soft and hard tissue regeneration (skin, bone, and cartilage) were discussed in detail.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"245-272"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing the Female Reproductive System Research with Additive Manufacturing.","authors":"Zhixin Du, Pengbei Fan, Liping Yang, Junlin Hou, Xiaodan Du, Yaohui Wang, Yujie Wang, Yulong Wang, Lingling Li","doi":"10.1177/19373341251359111","DOIUrl":"10.1177/19373341251359111","url":null,"abstract":"<p><p>The female reproductive system is highly complex, making it essential for applied research and translational medicine to accurately model its intricate physiological functions or develop strategies for restoring them. However, significant structural and functional differences between human and animal models, along with the limitations of static 2D cell culture technologies, underscore the need for more dynamic and sophisticated <i>in vitro</i> platforms, as well as <i>in vivo</i> therapies. These advancements are critical for deepening our understanding of reproductive biology and supporting clinical applications. Recent advancements in additive manufacturing technology have opened new frontiers in the study of the female reproductive system. By introducing diverse preclinical models and expanding the range of potential applications, this field has reached new heights, with the rapidly evolving research paradigm reshaping the scientific landscape. This review aims to summarize the growing body of evidence surrounding bioengineering strategies, platforms, and therapies in female reproductive medicine, with the goal of advancing our understanding of female reproductive biology and providing new avenues for fertility restoration. Specifically, we will examine the historical development, technological innovations, and scientific research related to the creation of 3D-engineered tissues for reconstructing the female reproductive system.Impact StatementThis review aims to summarize the growing body of evidence surrounding bioengineering strategies, platforms, and therapies in female reproductive medicine, with the goal of advancing our understanding of female reproductive biology and providing new avenues for fertility restoration. Specifically, the historical development, technological innovations, and scientific research related to the 3D-engineered tissues for reconstructing the female reproductive system were summarized. This review would help the audience, especially bioengineers who study the female reproductive system disease, as well as obstetricians and gynecologists, understand the possible application of additive manufacturing and acquire the strategies to engineer the female reproductive system <i>in vitro</i>.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"313-326"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Progress in Tissue Engineering of Temporomandibular Joint Condylar Cartilage.","authors":"Jinjin Ma, Yan Feng, Xinxin Ni, Qing Yang, Jun Lin","doi":"10.1089/ten.teb.2025.0073","DOIUrl":"10.1089/ten.teb.2025.0073","url":null,"abstract":"<p><p>The temporomandibular joint (TMJ) comprises the mandibular condyle, the articular surface of the temporal bone, and the articular disc. The articular cartilage in the TMJ is classified as fibrocartilage, which has distinct zones: the fibrous, proliferative, mature, and hypertrophic zones. TMJ osteoarthritis (TMJOA) is a prevalent condition affecting the TMJ, with its pathogenesis involving multiple factors such as trauma, occlusal instability, joint overload, and others. Current treatment options encompass noninvasive, minimally invasive, and surgical interventions. However, no definitive cure has been found. Tissue engineering offers a novel approach to treating TMJOA by promoting cartilage repair and regeneration by constructing artificial cartilage grafts made from a combination of cells, bioactive factors (BFs), and biodegradable scaffolds. Among the scaffolds commonly used in research are hydrogels, nanoparticles, and three-dimensional-printed structures, with mesenchymal stem cells serving as the primary cell source. Additionally, exosomes and gene therapy have shown promise in TMJOA treatment. Despite significant progress, optimizing the integration of seed cells, BFs, and scaffold materials remains a critical focus for future research. This article provides an in-depth review of the latest advancements in TMJ condylar cartilage tissue engineering.Impact StatementThis review comprehensively overviews tissue engineering advancements for temporomandibular joint condylar cartilage regeneration. It highlights key progress in scaffolds, cell-based therapies, bioactive factors, and gene therapies. The review offers valuable insights for future research and potential clinical applications, contributing significantly to developing novel therapeutic strategies for temporomandibular joint osteoarthritis.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"296-312"},"PeriodicalIF":4.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric Analysis and Trends of Organoid Technology in Tissue Engineering and Disease Modeling.","authors":"Die Hu, Lei Sun, Xuekun Xing","doi":"10.1177/19373368261469465","DOIUrl":"https://doi.org/10.1177/19373368261469465","url":null,"abstract":"<p><p>Organoid technology, as an innovative three-dimensional (3D) cell culture method, has undergone rapid advancement in the fields of tissue engineering and disease model construction in recent years. Using data retrieved from the PubMed database, this study employed bibliometric methods to systematically analyze 774 relevant publications from 2006 to 2026, focusing on research output, national and institutional distribution, collaboration networks, journal and author influence, and keyword evolution. The results show a significant increase in organoid research output, with China and the United States being the main contributing countries, and active international collaboration. Leading institutions, such as Harvard Medical School, occupy prominent positions in this field, with research covering multidisciplinary directions including cell culture, tissue scaffolds, microfluidic chips, and 3D bioprinting. Keyword analysis revealed core hotspots such as cell differentiation, tissue engineering methods, and multipotent stem cells, with 3D printing and induced pluripotent stem cells emerging as new research frontiers. The study indicates that organoid technology demonstrates substantial utility in simulating human tissue microenvironments, advancing precision medicine, and drug screening. Despite challenges related to vascularization, production standardization, and cost constraints, the development of high-throughput screening platforms and the enhancement of international collaboration systems will promote its clinical translation and sustainable development.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261469465"},"PeriodicalIF":4.3,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}