Tissue Engineering. Part B, Reviews最新文献

{"title":"Natural Plant-Derived Materials in Bone Tissue Engineering: Mapping Publication Status and Exploring Research Hotspots.","authors":"Xuefei Fu, Meng Zhang, Jiafeng Miao, Jiayi Li, Nan He, Hao Li, Lin Lu","doi":"10.1177/19373368261491566","DOIUrl":"https://doi.org/10.1177/19373368261491566","url":null,"abstract":"<p><p>Natural plant-derived materials are increasingly investigated for bone tissue engineering because they combine renewability, structural tunability, and compatibility with biomimetic scaffold design. This study mapped the publication status, intellectual base, and emerging themes of the field through a bibliometric analysis of Web of Science Core Collection records published from 1996 to 2026. The expanded search retrieved 2,198 records; after eligibility screening, 2,024 articles and reviews were retained. Annual output remained limited before 2011, increased after 2012, and accelerated after 2017, reaching 198 publications in 2025, while 152 publications had already been indexed for the partial year of 2026. After Taiwan was merged with China for country-level analyses, China was the largest publication center, followed by the United States, India, Iran, and South Korea. The University of Minho was the most productive institution, and International Journal of Biological Macromolecules was the leading publication source. Cocitation and keyword analyses indicate that the field is grounded in scaffold architecture, biomineralization, cellulose and nanocellulose, bacterial cellulose, natural polymer composites, and systems for bone regeneration and localized delivery. Recent bursts in antibacterial materials, three-dimensional printing, scaffolds, bone repair, and alginate point toward multifunctional, manufacturable, and clinically adaptable platforms. These findings provide an updated knowledge map and identify priorities for sustainable and translational bone-regeneration strategies.Impact StatementNatural plant-derived materials are increasingly positioned as sustainable building blocks for bone tissue engineering, yet the field remains fragmented across biomaterials, polymer science, nanotechnology, and regenerative medicine. This bibliometric study maps the global evolution, intellectual structure, and emerging directions of research on plant-derived materials for bone regeneration from 1996 to 2026. By identifying the central roles of cellulose-based materials, biomineralized composites, scaffold architecture, localized delivery, antibacterial design, and additive manufacturing, this work clarifies how the field is shifting from passive structural matrices toward multifunctional and clinically adaptable regenerative platforms. The findings provide researchers with an evidence-based knowledge map to guide material selection, scaffold design, interdisciplinary collaboration, and the translation of renewable bio-based materials into effective bone-repair strategies.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261491566"},"PeriodicalIF":4.3,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Strategies for Bioprinting Organized Musculoskeletal Tissues with Biomimetic Collagen Architecture.","authors":"Aliaa S Karam, Gabriela S Kronemberger, Daniel J Kelly","doi":"10.1177/19373368261486722","DOIUrl":"https://doi.org/10.1177/19373368261486722","url":null,"abstract":"<p><p>Collagen fiber organization is critical to the biomechanical function of musculoskeletal tissues. This has motivated increased interest in tissue engineering strategies aiming to recapitulate native collagen architecture in order to generate more biomimetic grafts. In particular, three-dimensional (3D) bioprinting has emerged as a powerful biofabrication platform capable of generating patient-specific constructs containing cells, supporting biomaterials, and diverse regulatory factors (e.g., genes, proteins, and small molecules). This paper will review how emerging bioprinting strategies can also be used to provide spatially defined external and/or internal biophysical cues to cells, thereby modulating their phenotype and the organization of the extracellular matrix they produce in culture. Furthermore, we will explore how postbioprinting interventions such as temporal enzymatic treatments and mechanical stimulation can be used to further promote cell and collagen alignment, matrix remodeling, and tissue maturation. Together, these approaches hold significant promise for the development of next-generation grafts for the treatment of diverse musculoskeletal injuries.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261486722"},"PeriodicalIF":4.3,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paradigm Shift Required to Achieve Long-Term Functional Cartilage Regeneration.","authors":"Zigang Ge, Xinyi Liu","doi":"10.1177/19373368261469777","DOIUrl":"https://doi.org/10.1177/19373368261469777","url":null,"abstract":"","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261469777"},"PeriodicalIF":4.3,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effects of Therapeutic Ultrasound and Biomaterials in Osteoarthritis.","authors":"Wenjie Hou, Xiaoxia Hao, Chunran Pan, Xingru Shang, Tao Xu","doi":"10.1177/19373368251398336","DOIUrl":"10.1177/19373368251398336","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a common degenerative joint disease characterized by progressive cartilage degradation, subchondral bone remodeling, and synovial inflammation. Current treatments cannot halt or reverse OA progression, necessitating the development of novel noninvasive therapies. Therapeutic ultrasound (US), particularly low-intensity pulsed US, has demonstrated efficacy in slowing OA progression. Therapeutic US generates significant thermal and nonthermal effects through noninvasive mechanical forces, exerting biological effects and regulating cell behavior. Therapeutic US has been explored for bone and cartilage repair and shows broad potential in tissue repair when combined with biomaterials. This review summarizes the enhanced or synergistic effects of US and biomaterials in OA. This study elucidated the molecular mechanisms underlying the effects of US on synovium, cartilage, subchondral bone, and mesenchymal stem cells. Notably, the combination of US with various biomaterials can modulate cellular behavior in OA through synergistic effects, including tissue regeneration, enhanced mechanical stimulation, drug delivery, and microenvironment regulation. For each cell type, we summarize the biological mechanisms underlying the therapeutic effects of US and biomaterials, demonstrating their potential to mitigate OA progression. Furthermore, this article explores the limitations and future research prospects of combining US and biomaterials as a therapeutic strategy. Overall, the integration of US and biomaterials holds significant promise as a novel treatment for OA, with potential applications in broader musculoskeletal tissue repair and regenerative medicine.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"369-389"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances Focusing on the Application of Various Ions in Tendon-Bone Healing.","authors":"Panpan Yu, Jiamin Guo, Guiying Nie, Yinling He, Tianhong Peng, Xi Chen, Liang Li, Zhu Dai, Wei Xie","doi":"10.1177/19373368251388823","DOIUrl":"10.1177/19373368251388823","url":null,"abstract":"<p><p>The tendon-bone interface (TBI) possesses a highly intricate structure, making complete restoration of its native structure postinjury particularly challenging, which often leads to suboptimal healing outcomes. Metal ions, such as calcium (Ca²⁺), magnesium (Mg²⁺), zinc (Zn²⁺), copper (Cu²⁺), cobalt (Co²⁺), strontium (Sr²⁺), iron (Fe^2+/Fe³⁺), and lithium (Li⁺), have attached significant attention in tissue regeneration research owing to the excellent roles in promoting angiogenesis, osteogenesis, and chondrogenesis. This review systematically elucidates a comprehensive overview of the current understanding of these bioactive ions' mechanisms and their applications in TBI repair. Additionally, the review highlights the importance of incorporating metal ions into biomaterial scaffolds to enhance simultaneous multitissue regeneration while addressing current therapeutic limitations in TBI management. Finally, the review outlines future research directions for optimizing ion-based biomaterial strategies to advance TBI treatment paradigms.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"359-368"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances of Cell Printing Technology in Organoid Engineering.","authors":"Yu-Han Ho, Yuanhong Liao, Lingni Liao, Tianjiao Mao, Yimin Guan, Ren Xu","doi":"10.1089/ten.teb.2025.0048","DOIUrl":"10.1089/ten.teb.2025.0048","url":null,"abstract":"<p><p>Organoid engineering is a rapidly expanding field that involves developing miniaturized, three-dimensional (3D) structures to mimic the architecture and function of real organs. It provides a powerful platform to investigate organ development, disease modeling, and personalized medicine. Recent advances in cell printing technology, also known as bioprinting, feature high-throughput potential, precise control, and enhanced reproducibility, enabling the deposition of living cells to generate complex, 3D biological structures. Cell printing with bioinks composed of cells and supportive biomaterials has been utilized to generate <i>in vitro</i> tissues and organs with intricate architectures and functionalities to investigate normal tissue morphogenesis and disease progression. The integration of cell printing technology and organoid engineering holds tremendous potential in biomedical research. Here, we summarize recent advances in cell printing technology in developing different organoid models, creating patient-specific tissue grafts, and utilizing these models and grafts in drug testing, as well as studying disease progression. Some of these bioprinted organoids have been utilized in clinical trials, highlighting the potential of cell printing technology in future applications in tissue and organ transplantation, as well as precision medicine.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"348-358"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Regulation of Tissue Remodeling Through Chitosan-Based Hydrogels in Wound Healing Dynamics.","authors":"Reyhaneh Molaei, Atefe Hosseinkhani, Mostafa Saberian","doi":"10.1089/ten.teb.2025.0078","DOIUrl":"10.1089/ten.teb.2025.0078","url":null,"abstract":"<p><p>Effective wound healing hinges on a precisely orchestrated tissue remodeling process that restores both structural integrity and functionality. This review delineates the molecular mechanisms by which chitosan-based hydrogels revolutionize wound repair. Derived from natural chitin, chitosan uniquely combines robust antimicrobial, hemostatic, and biodegradable properties with the capacity to modulate critical intracellular signaling cascades-including transforming growth factor-β, mitogen-activated protein kinase, and PI3K/AKT. These dynamic interactions drive fibroblast proliferation, stimulate the strategic transition from type III to type I collagen deposition, and finely tune extracellular matrix reorganization, thereby mitigating excessive fibrosis and minimizing scar formation. Notwithstanding its considerable therapeutic promise, clinical translation of chitosan-based hydrogels is tempered by challenges in mechanical stability and controlled degradation. We propose that advanced material engineering-encompassing precision cross-linking, nanoparticle integration, and synergistic stem cell-based strategies-could surmount these limitations. This comprehensive synthesis of current molecular insights sets the stage for next-generation regenerative biomaterials, positioning chitosan-based hydrogels as a paradigm-shifting platform for achieving superior healing outcomes in complex clinical scenarios.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"333-347"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond Diffraction Limits: Volumetric Photopolymerization as a Pathway for Medical-Grade Tissue Engineering.","authors":"Alexander Yu Pulver, Natalie A Pulver, Roman E Tokmachev, Lyubov N Antakova, Maria A Emelianova, Rimma A Poltavtseva","doi":"10.1177/19373368261491564","DOIUrl":"https://doi.org/10.1177/19373368261491564","url":null,"abstract":"<p><p>Regenerative medicine faces a systemic crisis from a fundamental technological mismatch. The goal is to engineer vascularized tissues, yet prevailing bioprinting paradigms are inadequate. Dominant additive techniques have a resolution limit (∼150-200 µm) and layer artifacts, far exceeding capillary diameters (5-7 µm) and native ECM scale. We argue that refining these methods is a dead end, ignoring the need for submicron (<1 µm) fidelity. This analysis critically evaluates volumetric alternatives for simultaneous 3D solidification. Within this impasse, we posit that pulsed holographic photopolymerization offers a viable theoretical pathway for instant fabrication of complex, submicron \"histionic scaffolds.\" A recent breakthrough introduced digital incoherent synthesis of holographic light fields, achieving millimeter-scale fabrication within 0.6 s at 11-19 µm resolution-the first practical implementation approaching medical-grade requirements. However, it still falls short of true submicron fidelity and lacks multimaterial capability. Acoustic holographic bioprinting, despite its potential, is fundamentally limited by acoustic diffraction, achieving only >100 µm resolution in biomaterials. Achieving the target medical resolution of 0.1-0.2 µm would require gigahertz frequencies, causing catastrophic signal attenuation and making the method unsuitable for volumetric tissue engineering. The emerging linear volumetric method of xolography represents another promising, though currently limited (∼5 µm), direction. The field is also crowded with other technologically complex but flawed trends-like endoscopic <i>in vivo</i> printing or advanced robotic extrusion-that simulate progress by repackaging methods with unsuitable resolution, diverting crucial resources. We further propose a novel two-stage paradigm using a prepolymerization \"morphogenetic matrix\" to solve the multimaterial problem. The primary impediment is no longer just physics but a critical deficit in funding for fundamental research, exacerbated by the dominance of short-term, simulacral projects. Therefore, advancing beyond this impasse requires a rigorous conceptual shift and a recommitment to foundational science.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261491564"},"PeriodicalIF":4.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle Organoids and Organs-on-Chips: A 15-Year Global Bibliometric Perspective.","authors":"Zhongxu Lv, Yuhao Yang, Ziqi Zhou, Zhuojian Cai, Liangbin Zhou, Li Jiang, Yiting Lei, Zhong Alan Li, Jia Li, Xiaoshuai Wang","doi":"10.1177/19373368261486378","DOIUrl":"https://doi.org/10.1177/19373368261486378","url":null,"abstract":"<p><p>Muscle organoids and Organs-on-Chips (OoCs) have emerged as advanced strategies in regenerative medicine and preclinical investigation for muscular diseases. Here, we aim to conduct a comprehensive bibliometric analysis to reveal the global research trends and their evolution in these fields. We retrieved studies on muscle organoids and OoCs published between 2010 and 2025 were retrieved from the Web of Science Core Collection (WoSCC), Scopus, and PubMed databases. Quantitative mapping of publication trends, academic impact, and research hotspots was conducted using R-bibliometrix, VOSviewer, and CiteSpace. A total of 2,083 articles (cardiac, <i>n</i> = 990; smooth, <i>n</i> = 921; and skeletal, <i>n</i> = 261, subgroups are nonexclusive categories) were included. Global publications have expanded rapidly since 2016, achieving an average annual growth rate of 26.0% and peaking in 2025. The United States and China emerged as leading countries in both publication and citation numbers, and Harvard University took the lead among different institutions. Thematic analysis revealed a developmental trajectory evolving from fundamental material engineering (e.g., biosensors) toward functional validation (e.g., drug screening) and sophisticated microenvironment reconstruction (e.g., vascularization and barrier functions). Furthermore, multidimensional metrics suggested a synchronized pattern of academic impact and developmental focus across all three muscle types. This bibliometric analysis reveals a thematic evolution in research priorities, moving beyond generic microfluidic design to addressing systemic tissue engineering bottlenecks, specifically perfusable vascularization, neuromuscular innervation, and multilineage maturation. These findings offer a bibliometric overview that can inform future efforts toward platform standardization, high-throughput screening, and clinical translation for muscular diseases.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261486378"},"PeriodicalIF":4.3,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing Tumor-on-a-Chip Systems with 3D Printing Technology.","authors":"Yandong Xie, Yanwen Su, Yuyang Gu, Kaili Liu, Xiaoyu Guo, Chaoyin Jia, Xiao Li, Jiankang He, Dichen Li","doi":"10.1177/19373368261486383","DOIUrl":"https://doi.org/10.1177/19373368261486383","url":null,"abstract":"<p><p>Cancer remains a significant global health challenge, necessitating advanced <i>in vitro</i> models for understanding relevant mechanisms and developing effective treatment approaches. Considered as a new paradigm of <i>in vitro</i> models, tumor-on-a-chip technology has emerged with the potential to recapitulate tumor microenvironments. Accordingly, 3D printing technology has been increasingly utilized to construct tumor-on-a-chip models, offering notable capability of generating 3D physiological and pathological features. This article examines 3D printing technology-driven research progress on developing tumor-on-a-chip systems, featuring enhanced capability for representing pathophysiological complexity as well as supporting in-depth biomedical analysis. The technological foundations of 3D printing for generating tumor-on-a-chip systems are introduced, including various printable materials and diverse printing mechanisms. The typical biomimetic and supporting components generated via 3D printing are examined as key elements to improve the performance of tumor-on-a-chip systems. The applications of 3D printing-enabled tumor-on-a-chip systems are illustrated with studies of specific cancer types. Finally, the future outlook of tumor-on-a-chip systems with 3D printing technology regarding technological development and biomedical impact is discussed.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261486383"},"PeriodicalIF":4.3,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148991282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}