Advances of Cell Printing Technology in Organoid Engineering.

Abstract

Organoid engineering is a rapidly expanding field that involves developing miniaturized, three-dimensional (3D) structures to mimic the architecture and function of real organs. It provides a powerful platform to investigate organ development, disease modeling, and personalized medicine. Recent advances in cell printing technology, also known as bioprinting, feature high-throughput potential, precise control, and enhanced reproducibility, enabling the deposition of living cells to generate complex, 3D biological structures. Cell printing with bioinks composed of cells and supportive biomaterials has been utilized to generate in vitro tissues and organs with intricate architectures and functionalities to investigate normal tissue morphogenesis and disease progression. The integration of cell printing technology and organoid engineering holds tremendous potential in biomedical research. Here, we summarize recent advances in cell printing technology in developing different organoid models, creating patient-specific tissue grafts, and utilizing these models and grafts in drug testing, as well as studying disease progression. Some of these bioprinted organoids have been utilized in clinical trials, highlighting the potential of cell printing technology in future applications in tissue and organ transplantation, as well as precision medicine. Impact Statement This article summarizes recent advances in integrating cell printing technology with three-dimensional tissue culture to develop organoid models. It discusses the advantages and limitations of three bioprinting technologies used in cell and organoid printing. The review also highlights the significant potential of cell printing technology in organoid model development and its applications in biomedical research and drug screening.